103 results about "Endometrium" patented technology

The invention provides an endometrial mesenchymal stem cell-derived exosome and application thereof in preparation of a medicine for treating diabetic wounds, and belongs to the technical field of biological medicines. The endometrial mesenchymal stem cell-derived exosome provided by the invention can effectively promote HUVEC tube forming ability, promote wound angiogenesis of diabetic model mice, increase collagen deposition and reduce wound area, finally achieves the purpose of promoting healing of diabetic wounds, and solves the problem of limited treatment effect of exosomes in the prior art. The endometrial mesenchymal stem cell-derived exosome provided by the invention can be used as a medicine for promoting angiogenesis and wound healing to be applied to diabetes wound treatment, and also provides a new strategy for treatment of other chronic wounds including burn.

The invention discloses an endometrium sampler, which relates to the technical field of medical instruments and is technically characterized by comprising a protective sleeve, a movable tube, a brush rod, a top bead, bristles and a handle, the protective sleeve and the movable pipe are both of a hollow tubular structure, and the movable pipe is arranged in the protective sleeve and can move in the axial direction of the protective sleeve. The brush rod is arranged in the movable pipe, one end of the brush rod is fixedly connected with the handle, and the other end of the brush rod is fixedly connected with the top bead. The section, close to the top bead, of the brush rod is a brush rod front end; the bristles are attached to the front end of the brush rod and incline towards the top bead, and the top bead is of a semi-surrounding cover-shaped structure. During sampling operation, the handle is rotated to drive the front end of the brush rod to rotate, so that the bristles cover an inverted pear-shaped uterine cavity area; after the bristles finish sampling, the movable pipe is controlled to move towards the top bead, so that the bristles are folded and protected by the movable pipe; the sampling efficiency, the sample integrity and the diagnosis accuracy can be improved.

The invention provides an immunohistochemical method for antibody joint detection and a kit thereof, and particularly relates to the technical field of medical detection.The method comprises the steps that fresh endometrial lymph node tissue is subjected to frozen section, and a continuous section A and a continuous section B are obtained; 10% neutral buffer formalin is used for fixation; blocking by using a peroxidase blocking agent; respectively and independently dropwise adding a CK19 antibody and an MUC1 antibody on the continuous slice A and the continuous slice B; dropwise adding an HRP direct standard signal enhancer; after the DAB develops color, TBS is terminated, hematoxylin is used for redyeing, dehydration is carried out through absolute ethyl alcohol, and sheet sealing is carried out; the immunohistochemical method kit comprises a CK19 antibody, an MUC1 antibody, an HRP direct standard signal enhancer, a peroxidase blocking agent, a DAB developing solution and a TBS cleaning solution. The kit is used for rapid diagnosis of endometrial lymph node micro metastasis, the whole process only needs 15 minutes, and antigen repair is not needed.

The invention is suitable for the technical field of bioengineering, and provides an endometrial organ model based on a micro-fluidic chip technology and a construction method thereof. According to the invention, an endometrial organ dynamic culture system based on a micro-fluidic chip technology is constructed, and the system can accurately simulate the in-vivo physiological microenvironment of endometrium, including key factors such as periodic mechanical stress, fluid shear force and intercellular interaction. Meanwhile, by comparing traditional 3D static culture with a micro-fluidic chip dynamic culture system, the remarkable advantages of dynamic culture in the aspects of organoid morphology, function and the like are defined, and the unique value of a micro-fluidic technology in construction of a highly-bionic endometrial organoid model is deeply reflected. An endometrial organ model constructed by the dynamic culture system can provide a reliable in-vitro platform closer to an in-vivo real state for pathogenesis research, drug screening, regenerative medicine research and the like of endometrial related diseases, and basic research and transformation application in related fields are assisted.

The invention relates to the field of biomarkers, and particularly provides a polycystic ovarian syndrome pregnancy outcome diagnosis marker and application thereof. According to the present invention, the endometrial targeting metabonomics research is adopted to screen the disease-related potential metabolic markers such as N-Acetylaspartic acid, Suberic acid and isoallolithocholic acid, and the three metabolites are subjected to quantitative detection so as to accurately predict the pregnancy outcome of the polycystic ovarian syndrome patient, such that the pregnancy outcome of the polycystic ovarian syndrome patient can be accurately predicted, and the prediction of the pregnancy outcome of the polycystic ovarian syndrome patient can be easily achieved, such that the disease-related potential metabolic markers such as N-Acetylaspartic acid, suberic acid and isoallolithocholic acid can be provided. A new path is provided for early intervention of the disease state to improve the pregnancy outcome, and a new drug target is provided for evaluation of the endometrial function of the polycystic ovarian syndrome.

The invention provides application of injectable bioactive hydrogel in treatment of recurrent abortion, and relates to the technical field of biomedicine, and the technical key points are as follows: aldehydic hyaluronic acid and chitosan are connected in parallel, the injectable bioactive hydrogel is developed, and the hydrogel is loaded with LIF, VEGF, IL-11 and valproic acid to enhance regeneration activity. According to the application, the effect is evaluated in vitro through a specific verification experiment. Results show that the hydrogel prepared by the application shows rapid gelation, good biocompatibility and factor loading capacity, and enhances the proliferation of human endometrial stromal cells and the vascularization capacity of human umbilical vein endothelial cells. In macrophages, the expression of a proinflammatory cytokine is down-regulated, and the expression of an anti-inflammatory cytokine IL-10 is up-regulated. In conclusion, the HA-CHO / chitosan hydrogel in the application supports endometrial regeneration by synergistically promoting proliferation, angiogenesis, immune regulation and receptivity.

The invention belongs to the field of medicines, and particularly provides application of mesenchymal stem cells in a medicine for treating thin endometrium and uterine cavity adhesion. The invention further provides a PDGF-BB monoclonal antibody, and the antibody can be specifically combined with PDGF-BB so as to inhibit corresponding protein activity. After the monoclonal antibody is used independently or in combination with stem cells, the effects of effectively promoting gland growth and reducing the fibrosis degree can be achieved, which shows that the monoclonal antibody can be used independently or in combination with the stem cells for treating thin endometrium and uterine cavity adhesion, and the application prospect is wide.

The invention relates to the technical field of modernization of traditional Chinese medicines, in particular to a nano-vesicle, a preparation method and application of the nano-vesicle in preparation of an endometrial injury repairing medicine. The preparation method comprises the following steps: taking a periplaneta americana ethanol extracting solution, and adding a buffer solution to prepare diluted homogenate; performing differential centrifugal treatment on the homogenate; filtering the supernatant obtained by the differential centrifugation treatment, and then centrifuging for 60-120 minutes by 100000g of the supernatant to obtain a precipitate; and re-suspending the precipitate to obtain the periplaneta americana ethanol extract source nano-vesicles. The periplaneta americana ethanol extract is used as a starting material to enrich the nano-vesicles for the first time, the combined process of differential centrifugation, membrane filtration and ultracentrifugation is adopted, the obtained nano-vesicles can be applied to the field of endometrial injury repair, especially treatment of gynecological diseases such as intrauterine adhesion and infertility with thin intima, and the blank in the technical field is filled.

The application discloses a detection method of peristalsis parameters, a detection device and a storage medium. The method comprises the following steps: emitting ultrasonic waves to an endometrium in at least two emission directions, and receiving echoes of the ultrasonic waves in the same direction as the emission direction to obtain at least two groups of echo signals, wherein each group of echo signals corresponds to each emission direction and comprises echo signals at at least two time points; calculating a peristalsis motion component corresponding to the endometrium according to each group of echo signals; and determining peristalsis parameters of the endometrium according to the peristalsis motion component. The detection method can improve the accuracy of the detected peristalsis parameters.

The invention discloses a muscle tissue information extraction method, system and device and a medium, and relates to the technical field of image analysis. Comprising the steps of obtaining a muscular tissue slice image; key features of each pixel point in the slice image are extracted, and the probability value of each pixel point is calculated according to the key features; classifying the slice images according to a probability value calculation result to obtain the number of muscle cells and the total area of muscle tissues, and calculating the average area and the average diameter of each muscle cell; extracting the muscular fascia area in the slice image, subtracting the muscular fascia area from the total area of the muscular tissues and then subtracting the total area of the muscle cells to obtain the sum of the areas of the endometrium, and calculating the thickness of the endometrium of the muscle cells according to the area of the endometrium; integrating the number, the average area, the average diameter and the thickness of the endometrium of the muscle cells to complete information extraction of the muscle tissue. According to the method, the dependence on manual operation is reduced, and the information of the muscle cells can be rapidly and accurately extracted.

The present application relates to the field of biological medicine, and discloses a method for repairing female reproductive tract based on marine active substances, comprising the following steps: S1, providing a composition containing marine active substances, wherein the marine active substances comprise sea cucumber saponins, oyster polypeptides and brown alga polysaccharides; S2, formulating the composition into a topical gel preparation; S3, applying the topical gel preparation to the female reproductive tract through vaginal or intrauterine administration to perform endometrial regeneration, scar reduction or inflammation microenvironment improvement, wherein the sea cucumber saponins are derived from Holothuria scabra; the oyster polypeptides are derived from Crassostrea gigas; and the brown alga polysaccharides are derived from Sargassum thunbergii and are sulfated polysaccharides. By combining sea cucumber saponins, oyster polypeptides and brown alga polysaccharides in a specific ratio (0.5-1.2%, 1-2.5%, 2-4%), the repairing effect of the female reproductive tract is significantly improved, which is superior to single-component gel or existing hyaluronic acid gel.

The invention provides an antibacterial peptide RB3 and application thereof. The amino acid sequence of the antibacterial peptide RB3 is as shown in SEQ ID NO: 1. The antibacterial peptide RB3 has remarkable antibacterial activity on five endometritis pathogenic bacteria, namely, E. coli, K. pneumoniae, P. mirabilis, P. aeruginosa and E. faecium, can be used for inhibiting the growth and proliferation of the endometritis pathogenic bacteria and maintaining a normal flora structure and an immune microenvironment of endometrium, and can be used for preparing the antibacterial peptide RB3. The antibacterial peptide RB3 has the advantages that the antibacterial peptide RB3 has obvious antibacterial activity on the five endometritis pathogenic bacteria, namely, E. coli, K. pneumoniae, P. mirabilis, P. aeruginosa, P. mirabilis, P. aeruginosa and E. faecium; according to the present invention, the antibacterial peptide RB3 can be directly obtained through solid-phase synthesis, such that the effective prevention and treatment of endometritis can be achieved, the important significance on the prevention and the clinical treatment of endometritis can be provided, and the antibacterial peptide RB3 can be directly obtained through solid-phase synthesis so as to easily achieve the large-scale preparation and the clinical large-scale application of the antibacterial peptide RB3.

The present invention relates to a device suitable for introducing a fertilized egg or embryo into a maternal endometrium. The device includes an elongate body including a distal tip and a cavity extending through the distal tip and to an aperture of the distal tip. The cavity is configured to receive and expel fertilized eggs or embryos through the aperture. The plunger is configured to be slidably received within the cavity. The distal tip includes a piercing portion for piercing a maternal endometrium, and the aperture is concave.

The present application relates to the technical field of pharmaceutical preparation, in particular to an ultrasonic imaging composite gel, a preparation method and application thereof. The present application provides an ultrasonic imaging composite gel, which comprises a gel matrix, an ultrasonic imaging material and a pharmaceutically acceptable solvent according to mass fraction; the concentration of the gel matrix in the ultrasonic imaging composite gel is 1-500 mg / mL; the concentration of the ultrasonic imaging material in the ultrasonic imaging composite gel is 1-500 mg / mL; the gel matrix comprises one or more of a temperature-sensitive gel matrix, an injectable gel matrix and an ion-responsive gel matrix; the ultrasonic imaging material comprises an ultrasonic phase transition imaging agent and a polymer material wrapping the ultrasonic phase transition imaging agent, and the ultrasonic imaging material is an ultrasonic imaging microbubble structure. The ultrasonic imaging composite gel can enhance ultrasonic imaging in the process of embryo transplantation and promote the adhesion and implantation of embryos and endometrium.

Methods for treating hormonally driven disorders by administering the PI3K / mT0R inhibitor gedatolisib in combination with hormone therapy are provided. Hormonally driven disorders include, for example, prostate cancer and endometrial disorders. The treatment regimens can also include additional therapies, such as chemotherapy, surgery and / or administration of an immune checkpoint inhibitor(s), a receptor tyrosine kinase inhibitor, a CDK 4 / 6 inhibitor or an epigenetic-targeted therapy.

This invention provides a method for constructing a human endometrial organoid aging model, belonging to the field of biological model technology. Based on human endometrial organoids, this invention utilizes hydrogen peroxide (H2O2) stimulation to induce a human endometrial organoid aging model. This human endometrial organoid aging model clearly exhibits multiple cellular aging phenotypes, including slowed organoid growth rate, decreased cell proliferation index (Ki67), enhanced SA-β-gal activity, upregulated expression of cell cycle inhibitors P16 and P21, and significantly upregulated gene expression levels of aging-associated secretory phenotype (SASP) components. It can provide an effective tool for screening anti-aging drugs for women, high-throughput and high-content screening, personalized treatment strategy development, and mechanistic research.

The invention belongs to the field of biological medicines, and relates to a novel application of a cranberry extract. The invention provides an application of a cranberry extract in preparation of a medicine for preventing and treating polycystic ovarian syndrome (PCOS), and further provides an application of the cranberry extract in preparation of a medicine for preventing and treating thin endometrium related to the polycystic ovarian syndrome. The cranberry extract which is safe in source and definite in component is utilized, an overall solution which is clear in mechanism, achieves multi-target cooperation and can relieve PCOS symptoms and promote endometrial repair is provided for the first time, and the cranberry extract has important clinical significance. The cranberry extract contains six specific anthocyanins, has good potential in the aspect of improving PCOS and related thin endometrium problems, and provides new possibility for a treatment strategy of the disease.

The invention provides a construction method and application of an endometrial cancer CT image segmentation model. The construction method comprises the following steps: acquiring a plurality of endometrial CT images marked with canceration regions as a training data set; constructing an endometrial cancer CT image segmentation framework, wherein the endometrial cancer CT image segmentation framework is of a U-Net structure comprising a coding unit, a bottleneck layer and a decoding unit; and training the endometrial cancer CT image segmentation architecture by using the training data set to obtain an endometrial cancer CT image segmentation model. According to the scheme, multi-scale structure change information is explicitly captured by constructing a multi-direction coding structure response operator and generating a coding structure gradient field, and representation of thin and weak boundaries and small lesions is enhanced to improve the segmentation accuracy of the model.

The invention discloses an application of miR-146b-3p inhibitor in improving sheep embryo implantation abnormity caused by LPS (Lipopolysaccharide), and belongs to the technical field of biology, and the sequence of the miR-146b-3p inhibitor is shown as SEQ ID No.1. The invention further discloses a preparation method of the miR-146b-3p inhibitor. LPS can cause sheep endometrial epithelial cell inflammatory factor abnormity, adhesion molecule expression change, trophoblast sphere adhesion growth inhibition and miR-146b-3p expression increase, miR-146b-3p inhibits GYS2 gene expression in a targeted manner, endometrial glycogen synthesis is reduced, and embryo implantation is affected. The miR-146b-3p inhibitor can be used for rescuing GYS2 expression reduction caused by LPS (Lipopolysaccharide), relieving glycogen synthesis reduction and expression inhibition of an attachment key gene, promoting sheep embryo attachment and reducing the embryo loss rate. The invention further provides a reagent containing the inhibitor, a reagent for overexpressing the GYS2 gene, a method for promoting embryo implantation and related products, a new direction is provided for improving the reproductive performance of sheep, and the inhibitor has important value and significance for the sheep raising industry.

Provided herein is a targeting molecule, wherein the targeting molecule binds to a surface molecule on an endometrial endothelial cell (EEC) in which expression of the surface molecule is upregulated.

The invention relates to composite hydrogel for promoting endometrial regeneration and resisting fibrosis as well as a preparation method and application of the composite hydrogel. The composite hydrogel consists of a deciduous endometrial decellularized extracellular matrix pre-gel solution and anti-fibrosis drug loaded polymer sustained-release microspheres dispersed in the deciduous endometrial decellularized extracellular matrix pre-gel solution, the material can be gelated in situ at body temperature and has a shear thinning characteristic. According to the invention, dECM with'decidualization energization 'is combined with an anti-fibrosis drug sustained release system to synergistically solve the two problems of insufficient endometrial regeneration and excessive fibrosis, and experiments prove that the dECM can significantly promote functional endometrial regeneration and restore fertility, and has important application value in drugs or medical instruments for treating intrauterine adhesion.

The application discloses a medical instrument technical field, and relates to an embryo transfer device, which comprises a sleeve, an inner core and a syringe, the inner core is detachably connected with the syringe, the tail section of the inner core is provided with a circular arc structure at an opening, the thickness of the tube wall of the opening is gradually reduced from the opening to the tail section, and the inner wall of the tube wall of the opening is provided with a funnel structure; the syringe comprises a barrel, a pressurizing barrel is connected to the barrel, the inner diameter of the inner cavity of the barrel is smaller than the inner diameter of the inner cavity of the pressurizing barrel, a piston plate is arranged in the barrel, the piston plate is in sliding fit with the inner wall of the barrel, the pressurizing barrel is in communication with the barrel, a push plate is arranged in the pressurizing barrel, a push rod is connected to one side of the push plate, and the push rod penetrates through the pressurizing barrel. The special syringe is designed, the syringe and the inner core are used in cooperation, the embryo can be better controlled in suction and injection, the damage to the endometrium is reduced, the success rate and safety of embryo transfer are improved, and better treatment options are provided for infertile patients.

The invention relates to a construction method of a bionic endometrial organ, which comprises the following steps: mixing three primary cells to obtain a mixed cell precipitate, and adding the mixed cell precipitate into a bionic organ culture medium; precipitating and resuspending the mixed cells to obtain a cell suspension; and adding Matrigel, and transferring into a constant-temperature cell culture box for culturing, so as to obtain the bionic endometrial organ. The regeneration promoting effect of the bionic endometrial organ provided by the invention is obviously better than that of transplanted pure stromal cells and pure epithelial organ groups, and the endometrial organ of the bionic cells has a huge application value in the aspect of tissue regeneration. According to the construction method of the intrauterine adhesion disease model, a fibrosis inducing factor TGF beta 1 is added into a bionic endometrial organ, immunofluorescent staining of a fibrosis marker alpha SMA is carried out, and the intrauterine adhesion disease model is obtained. By reproducing the pathological change of the intrauterine adhesion inner membrane, the model can be used as an intrauterine adhesion pathological model for disease pathological research or drug screening research.

The present invention discloses an epidermal growth factor - linker polypeptide - collagen, and the linker polypeptide has an amino acid sequence as shown in SEQ ID No.1. In the present invention, a linker polypeptide capable of connecting EGF and collagen is designed and synthesized. The N - terminal of the linker polypeptide mimics the EGF receptor structure and can bind to EGF, and the connection between the linker polypeptide and EGF is through non - covalent van der Waals forces, which will not destroy the structure of EGF; the C - terminal of the linker polypeptide is rich in lysine residues and can be covalently cross - linked to collagen through transglutaminase to form EGF - linker polypeptide - collagen. The protein solution with the above - mentioned EGF - linker polypeptide - collagen as the active ingredient not only has low cytotoxicity and high biological safety, but also can significantly prolong the action time of epidermal growth factor. In addition, it can form a stable repair microenvironment on the surface of the endometrium and significantly improve the repair effect of endometrial injury.

The invention provides application of endometrial mesenchymal stem cell exosomes in atherosclerosis, and belongs to the technical field of biological medicine. The invention provides a bioactive preparation which is derived from specific tissues, has good safety and definite treatment directivity, can effectively intervene in the progress of atherosclerotic plaques and relieve pathological changes of blood vessels, and the treatment convenience and patient tolerance are remarkably improved through a non-invasive administration mode of the bioactive preparation. A brand new solution with excellent application potential is provided for clinical management of atherosclerosis.

The present invention discloses a culture medium for sheep endometrial organoids and a culture method thereof. The formulation of the culture medium includes a basal medium, cell growth factors, Wnt / β-catenin signaling pathway activators, BMP inhibitors, antioxidants and protectants, nutrients and cell culture additives, and auxiliary components. Culturing sheep endometrial organoids using the culture medium developed by the present invention can be used to optimize sheep breeding techniques, help study the improvement of the breeding efficiency and offspring quality of sheep breeds, and has potential economic value.

The utility model relates to the technical field of medical treatment, in particular to an endometrial pathological section rapid sealing device which comprises a base, and an L-shaped working table is arranged on the top of the base. By means of an assembly composed of the L-shaped working table, the first slice turning assembly, the second slice turning assembly, the conveying frame, the slice covering assembly and the quartz heating pipe, a set of automatic rapid slice sealing equipment is provided for endometrial pathological slices, a traditional manual slice sealing operation mode is replaced, the hands of personnel are effectively liberated in the slice sealing period, and the working efficiency is improved. According to the device, the labor force of medical staff is reduced, manual work is replaced with mechanical parts, continuous piece feeding, glue dripping, piece covering, drying and other work are carried out, slices can be rapidly sealed, glue dripping and piece covering can be continuously carried out on glass slides passing through the conveying frame in the piece sealing period, auxiliary heating and drying treatment can be carried out on the pieces after piece covering is completed, and the production efficiency is improved. And the drying efficiency of the resin adhesive is improved, and later-stage centralized collection and transfer of personnel are facilitated.

The invention belongs to the technical field of natural products and pharmaceutical industry, and provides a compound for improving endometrial aging. The compound is ganoderic acid C2 and is screened by the following method: inducing an endometrial organ to form an aging endometrial model by using D-galactose; contacting a compound to be detected with the senescence endometrial model, and detecting the proportion of beta-galactosidase and / or EdU positive cells; and screening a compound capable of remarkably reducing beta-galactosidase and / or remarkably improving the proportion of EdU positive cells, namely the compound for improving endometrial aging. The model constructed by the invention can fully simulate the physiological status and pathological change of the senescent endometrium, the screening reliability is high, the period is short, the ethical problem is avoided, and a reliable method is provided for subsequent screening of natural products.

The utility model relates to a medical anti-adhesion film wrapped silicon rubber stent for preventing adhesion and draining after a uterine cavity operation. The medical anti-adhesion film wrapped silicon rubber stent consists of a triangular silicon rubber stent frame (2) with a mould pressing flash film (1), a wrapped medical anti-adhesion film (3), an anti-adhesion composite stent (4), an assembling pull wire (7) and a connecting silicon rubber drainage tube (8), the device is used for preventing endometrial adhesion and draining after the uterine cavity operation. The configuration and the flexible mechanical property of the anti-adhesion composite stent (4) are matched with the anatomical form of the uterine cavity, and a perfect physical barrier isolation effect is achieved. The surface of the medical anti-adhesion membrane (3) has good affinity with the inner membrane of an operative wound, so that the repair and regeneration of the inner membrane of the wound can be promoted. A surgeon can longitudinally lengthen the anti-adhesion composite stent (4) and transversely fold the anti-adhesion composite stent (4) into a slender strip shape by means of the soft plastic push rod (6) and the pull line (7), the anti-adhesion composite stent (4) is gently pushed into a uterine cavity, and the anti-adhesion composite stent (4) automatically retracts to restore a triangular structure after being released and is smoothly spread in the uterine cavity. And after treatment, the uterine cavity is gently pulled out.