69 results about "Endometrium" patented technology

The invention discloses an endometrium sampler, which relates to the technical field of medical instruments and is technically characterized by comprising a protective sleeve, a movable tube, a brush rod, a top bead, bristles and a handle, the protective sleeve and the movable pipe are both of a hollow tubular structure, and the movable pipe is arranged in the protective sleeve and can move in the axial direction of the protective sleeve. The brush rod is arranged in the movable pipe, one end of the brush rod is fixedly connected with the handle, and the other end of the brush rod is fixedly connected with the top bead. The section, close to the top bead, of the brush rod is a brush rod front end; the bristles are attached to the front end of the brush rod and incline towards the top bead, and the top bead is of a semi-surrounding cover-shaped structure. During sampling operation, the handle is rotated to drive the front end of the brush rod to rotate, so that the bristles cover an inverted pear-shaped uterine cavity area; after the bristles finish sampling, the movable pipe is controlled to move towards the top bead, so that the bristles are folded and protected by the movable pipe; the sampling efficiency, the sample integrity and the diagnosis accuracy can be improved.

The invention provides an immunohistochemical method for antibody joint detection and a kit thereof, and particularly relates to the technical field of medical detection.The method comprises the steps that fresh endometrial lymph node tissue is subjected to frozen section, and a continuous section A and a continuous section B are obtained; 10% neutral buffer formalin is used for fixation; blocking by using a peroxidase blocking agent; respectively and independently dropwise adding a CK19 antibody and an MUC1 antibody on the continuous slice A and the continuous slice B; dropwise adding an HRP direct standard signal enhancer; after the DAB develops color, TBS is terminated, hematoxylin is used for redyeing, dehydration is carried out through absolute ethyl alcohol, and sheet sealing is carried out; the immunohistochemical method kit comprises a CK19 antibody, an MUC1 antibody, an HRP direct standard signal enhancer, a peroxidase blocking agent, a DAB developing solution and a TBS cleaning solution. The kit is used for rapid diagnosis of endometrial lymph node micro metastasis, the whole process only needs 15 minutes, and antigen repair is not needed.

The invention belongs to the field of medicines, and particularly provides application of mesenchymal stem cells in a medicine for treating thin endometrium and uterine cavity adhesion. The invention further provides a PDGF-BB monoclonal antibody, and the antibody can be specifically combined with PDGF-BB so as to inhibit corresponding protein activity. After the monoclonal antibody is used independently or in combination with stem cells, the effects of effectively promoting gland growth and reducing the fibrosis degree can be achieved, which shows that the monoclonal antibody can be used independently or in combination with the stem cells for treating thin endometrium and uterine cavity adhesion, and the application prospect is wide.

The invention relates to the technical field of modernization of traditional Chinese medicines, in particular to a nano-vesicle, a preparation method and application of the nano-vesicle in preparation of an endometrial injury repairing medicine. The preparation method comprises the following steps: taking a periplaneta americana ethanol extracting solution, and adding a buffer solution to prepare diluted homogenate; performing differential centrifugal treatment on the homogenate; filtering the supernatant obtained by the differential centrifugation treatment, and then centrifuging for 60-120 minutes by 100000g of the supernatant to obtain a precipitate; and re-suspending the precipitate to obtain the periplaneta americana ethanol extract source nano-vesicles. The periplaneta americana ethanol extract is used as a starting material to enrich the nano-vesicles for the first time, the combined process of differential centrifugation, membrane filtration and ultracentrifugation is adopted, the obtained nano-vesicles can be applied to the field of endometrial injury repair, especially treatment of gynecological diseases such as intrauterine adhesion and infertility with thin intima, and the blank in the technical field is filled.

The application discloses a detection method of peristalsis parameters, a detection device and a storage medium. The method comprises the following steps: emitting ultrasonic waves to an endometrium in at least two emission directions, and receiving echoes of the ultrasonic waves in the same direction as the emission direction to obtain at least two groups of echo signals, wherein each group of echo signals corresponds to each emission direction and comprises echo signals at at least two time points; calculating a peristalsis motion component corresponding to the endometrium according to each group of echo signals; and determining peristalsis parameters of the endometrium according to the peristalsis motion component. The detection method can improve the accuracy of the detected peristalsis parameters.

The present application relates to the field of biological medicine, and discloses a method for repairing female reproductive tract based on marine active substances, comprising the following steps: S1, providing a composition containing marine active substances, wherein the marine active substances comprise sea cucumber saponins, oyster polypeptides and brown alga polysaccharides; S2, formulating the composition into a topical gel preparation; S3, applying the topical gel preparation to the female reproductive tract through vaginal or intrauterine administration to perform endometrial regeneration, scar reduction or inflammation microenvironment improvement, wherein the sea cucumber saponins are derived from Holothuria scabra; the oyster polypeptides are derived from Crassostrea gigas; and the brown alga polysaccharides are derived from Sargassum thunbergii and are sulfated polysaccharides. By combining sea cucumber saponins, oyster polypeptides and brown alga polysaccharides in a specific ratio (0.5-1.2%, 1-2.5%, 2-4%), the repairing effect of the female reproductive tract is significantly improved, which is superior to single-component gel or existing hyaluronic acid gel.

The present application relates to the technical field of pharmaceutical preparation, in particular to an ultrasonic imaging composite gel, a preparation method and application thereof. The present application provides an ultrasonic imaging composite gel, which comprises a gel matrix, an ultrasonic imaging material and a pharmaceutically acceptable solvent according to mass fraction; the concentration of the gel matrix in the ultrasonic imaging composite gel is 1-500 mg / mL; the concentration of the ultrasonic imaging material in the ultrasonic imaging composite gel is 1-500 mg / mL; the gel matrix comprises one or more of a temperature-sensitive gel matrix, an injectable gel matrix and an ion-responsive gel matrix; the ultrasonic imaging material comprises an ultrasonic phase transition imaging agent and a polymer material wrapping the ultrasonic phase transition imaging agent, and the ultrasonic imaging material is an ultrasonic imaging microbubble structure. The ultrasonic imaging composite gel can enhance ultrasonic imaging in the process of embryo transplantation and promote the adhesion and implantation of embryos and endometrium.

Methods for treating hormonally driven disorders by administering the PI3K / mT0R inhibitor gedatolisib in combination with hormone therapy are provided. Hormonally driven disorders include, for example, prostate cancer and endometrial disorders. The treatment regimens can also include additional therapies, such as chemotherapy, surgery and / or administration of an immune checkpoint inhibitor(s), a receptor tyrosine kinase inhibitor, a CDK 4 / 6 inhibitor or an epigenetic-targeted therapy.

This invention provides a method for constructing a human endometrial organoid aging model, belonging to the field of biological model technology. Based on human endometrial organoids, this invention utilizes hydrogen peroxide (H2O2) stimulation to induce a human endometrial organoid aging model. This human endometrial organoid aging model clearly exhibits multiple cellular aging phenotypes, including slowed organoid growth rate, decreased cell proliferation index (Ki67), enhanced SA-β-gal activity, upregulated expression of cell cycle inhibitors P16 and P21, and significantly upregulated gene expression levels of aging-associated secretory phenotype (SASP) components. It can provide an effective tool for screening anti-aging drugs for women, high-throughput and high-content screening, personalized treatment strategy development, and mechanistic research.

The invention belongs to the field of biological medicines, and relates to a novel application of a cranberry extract. The invention provides an application of a cranberry extract in preparation of a medicine for preventing and treating polycystic ovarian syndrome (PCOS), and further provides an application of the cranberry extract in preparation of a medicine for preventing and treating thin endometrium related to the polycystic ovarian syndrome. The cranberry extract which is safe in source and definite in component is utilized, an overall solution which is clear in mechanism, achieves multi-target cooperation and can relieve PCOS symptoms and promote endometrial repair is provided for the first time, and the cranberry extract has important clinical significance. The cranberry extract contains six specific anthocyanins, has good potential in the aspect of improving PCOS and related thin endometrium problems, and provides new possibility for a treatment strategy of the disease.

The invention provides a construction method and application of an endometrial cancer CT image segmentation model. The construction method comprises the following steps: acquiring a plurality of endometrial CT images marked with canceration regions as a training data set; constructing an endometrial cancer CT image segmentation framework, wherein the endometrial cancer CT image segmentation framework is of a U-Net structure comprising a coding unit, a bottleneck layer and a decoding unit; and training the endometrial cancer CT image segmentation architecture by using the training data set to obtain an endometrial cancer CT image segmentation model. According to the scheme, multi-scale structure change information is explicitly captured by constructing a multi-direction coding structure response operator and generating a coding structure gradient field, and representation of thin and weak boundaries and small lesions is enhanced to improve the segmentation accuracy of the model.

The invention discloses an application of miR-146b-3p inhibitor in improving sheep embryo implantation abnormity caused by LPS (Lipopolysaccharide), and belongs to the technical field of biology, and the sequence of the miR-146b-3p inhibitor is shown as SEQ ID No.1. The invention further discloses a preparation method of the miR-146b-3p inhibitor. LPS can cause sheep endometrial epithelial cell inflammatory factor abnormity, adhesion molecule expression change, trophoblast sphere adhesion growth inhibition and miR-146b-3p expression increase, miR-146b-3p inhibits GYS2 gene expression in a targeted manner, endometrial glycogen synthesis is reduced, and embryo implantation is affected. The miR-146b-3p inhibitor can be used for rescuing GYS2 expression reduction caused by LPS (Lipopolysaccharide), relieving glycogen synthesis reduction and expression inhibition of an attachment key gene, promoting sheep embryo attachment and reducing the embryo loss rate. The invention further provides a reagent containing the inhibitor, a reagent for overexpressing the GYS2 gene, a method for promoting embryo implantation and related products, a new direction is provided for improving the reproductive performance of sheep, and the inhibitor has important value and significance for the sheep raising industry.

Provided herein is a targeting molecule, wherein the targeting molecule binds to a surface molecule on an endometrial endothelial cell (EEC) in which expression of the surface molecule is upregulated.

The invention relates to composite hydrogel for promoting endometrial regeneration and resisting fibrosis as well as a preparation method and application of the composite hydrogel. The composite hydrogel consists of a deciduous endometrial decellularized extracellular matrix pre-gel solution and anti-fibrosis drug loaded polymer sustained-release microspheres dispersed in the deciduous endometrial decellularized extracellular matrix pre-gel solution, the material can be gelated in situ at body temperature and has a shear thinning characteristic. According to the invention, dECM with'decidualization energization 'is combined with an anti-fibrosis drug sustained release system to synergistically solve the two problems of insufficient endometrial regeneration and excessive fibrosis, and experiments prove that the dECM can significantly promote functional endometrial regeneration and restore fertility, and has important application value in drugs or medical instruments for treating intrauterine adhesion.

The application discloses a medical instrument technical field, and relates to an embryo transfer device, which comprises a sleeve, an inner core and a syringe, the inner core is detachably connected with the syringe, the tail section of the inner core is provided with a circular arc structure at an opening, the thickness of the tube wall of the opening is gradually reduced from the opening to the tail section, and the inner wall of the tube wall of the opening is provided with a funnel structure; the syringe comprises a barrel, a pressurizing barrel is connected to the barrel, the inner diameter of the inner cavity of the barrel is smaller than the inner diameter of the inner cavity of the pressurizing barrel, a piston plate is arranged in the barrel, the piston plate is in sliding fit with the inner wall of the barrel, the pressurizing barrel is in communication with the barrel, a push plate is arranged in the pressurizing barrel, a push rod is connected to one side of the push plate, and the push rod penetrates through the pressurizing barrel. The special syringe is designed, the syringe and the inner core are used in cooperation, the embryo can be better controlled in suction and injection, the damage to the endometrium is reduced, the success rate and safety of embryo transfer are improved, and better treatment options are provided for infertile patients.

The invention relates to a construction method of a bionic endometrial organ, which comprises the following steps: mixing three primary cells to obtain a mixed cell precipitate, and adding the mixed cell precipitate into a bionic organ culture medium; precipitating and resuspending the mixed cells to obtain a cell suspension; and adding Matrigel, and transferring into a constant-temperature cell culture box for culturing, so as to obtain the bionic endometrial organ. The regeneration promoting effect of the bionic endometrial organ provided by the invention is obviously better than that of transplanted pure stromal cells and pure epithelial organ groups, and the endometrial organ of the bionic cells has a huge application value in the aspect of tissue regeneration. According to the construction method of the intrauterine adhesion disease model, a fibrosis inducing factor TGF beta 1 is added into a bionic endometrial organ, immunofluorescent staining of a fibrosis marker alpha SMA is carried out, and the intrauterine adhesion disease model is obtained. By reproducing the pathological change of the intrauterine adhesion inner membrane, the model can be used as an intrauterine adhesion pathological model for disease pathological research or drug screening research.

The invention provides application of endometrial mesenchymal stem cell exosomes in atherosclerosis, and belongs to the technical field of biological medicine. The invention provides a bioactive preparation which is derived from specific tissues, has good safety and definite treatment directivity, can effectively intervene in the progress of atherosclerotic plaques and relieve pathological changes of blood vessels, and the treatment convenience and patient tolerance are remarkably improved through a non-invasive administration mode of the bioactive preparation. A brand new solution with excellent application potential is provided for clinical management of atherosclerosis.

The utility model relates to the technical field of medical treatment, in particular to an endometrial pathological section rapid sealing device which comprises a base, and an L-shaped working table is arranged on the top of the base. By means of an assembly composed of the L-shaped working table, the first slice turning assembly, the second slice turning assembly, the conveying frame, the slice covering assembly and the quartz heating pipe, a set of automatic rapid slice sealing equipment is provided for endometrial pathological slices, a traditional manual slice sealing operation mode is replaced, the hands of personnel are effectively liberated in the slice sealing period, and the working efficiency is improved. According to the device, the labor force of medical staff is reduced, manual work is replaced with mechanical parts, continuous piece feeding, glue dripping, piece covering, drying and other work are carried out, slices can be rapidly sealed, glue dripping and piece covering can be continuously carried out on glass slides passing through the conveying frame in the piece sealing period, auxiliary heating and drying treatment can be carried out on the pieces after piece covering is completed, and the production efficiency is improved. And the drying efficiency of the resin adhesive is improved, and later-stage centralized collection and transfer of personnel are facilitated.

The utility model relates to a medical anti-adhesion film wrapped silicon rubber stent for preventing adhesion and draining after a uterine cavity operation. The medical anti-adhesion film wrapped silicon rubber stent consists of a triangular silicon rubber stent frame (2) with a mould pressing flash film (1), a wrapped medical anti-adhesion film (3), an anti-adhesion composite stent (4), an assembling pull wire (7) and a connecting silicon rubber drainage tube (8), the device is used for preventing endometrial adhesion and draining after the uterine cavity operation. The configuration and the flexible mechanical property of the anti-adhesion composite stent (4) are matched with the anatomical form of the uterine cavity, and a perfect physical barrier isolation effect is achieved. The surface of the medical anti-adhesion membrane (3) has good affinity with the inner membrane of an operative wound, so that the repair and regeneration of the inner membrane of the wound can be promoted. A surgeon can longitudinally lengthen the anti-adhesion composite stent (4) and transversely fold the anti-adhesion composite stent (4) into a slender strip shape by means of the soft plastic push rod (6) and the pull line (7), the anti-adhesion composite stent (4) is gently pushed into a uterine cavity, and the anti-adhesion composite stent (4) automatically retracts to restore a triangular structure after being released and is smoothly spread in the uterine cavity. And after treatment, the uterine cavity is gently pulled out.

The application provides a method for constructing an endometrial carcinoma CT image segmentation model and application thereof, including the following steps: acquiring multiple endometrial CT images labeled with cancerous regions as a training data set; constructing an endometrial carcinoma CT image segmentation architecture, wherein the endometrial carcinoma CT image segmentation architecture is a U-Net structure including an encoding unit, a bottleneck layer and a decoding unit; and training the endometrial carcinoma CT image segmentation architecture with the training data set to obtain an endometrial carcinoma CT image segmentation model. The scheme explicitly captures multi-scale structural change information by constructing a multi-directional coding structure response operator and generating a coding structure gradient field, thereby strengthening the representation of thin and weak boundaries and small lesions to improve the segmentation accuracy of the model.

The invention belongs to the technical field of biological medicines, and discloses an endometrial organ and a construction method thereof. The invention provides a construction method of endometrial organs. An IM differential culture medium 1 containing L-alanyl-L-glutamine with specific concentration and CHIR 99021, an IM differential culture medium 2 containing L-alanyl-L-glutamine with specific concentration, bFGF and RA and an MD differential culture medium containing CHIR 99021 with specific concentration are adopted as a Miller tube cell induced differentiation culture system; the method can efficiently guide the differentiation of the human pluripotent stem cells to the Mullerian canal cells while maintaining the cell proliferation ability, the obtained Mullerian canal cells have excellent proliferation and differentiation ability, and an organoid with natural endometrial characteristics can be formed in an endometrial organoid induced differentiation culture system; the material can be used as an experimental material in research on development mechanism of endometrium, disease model establishment, drug screening and the like, and has a wide application prospect.

The application discloses a three-layer time sequence regulation type inflammation targeting composite repair system for repairing endometrial injury and a preparation method and application thereof. The system is a three-layer composite structure, which comprises, from outside to inside, (1) an outer ROS response hydrogel layer loaded with anti-inflammatory drugs, which can respond to early high expression of active oxygen to rapidly degrade the drugs, so that acute inflammation is inhibited; (2) a middle layer of targeted delivery and metabolic repair, which is a M1 macrophage membrane pseudo-loaded drug liposome (NMN@M-Lipo), which is exposed after the degradation of the outer hydrogel, actively targets the inflammation lesion through the M1 membrane, and slowly releases nicotinamide mononucleotide (NMN) to restore cell energy metabolism; and (3) an inner layer of cell recruitment and regeneration, which is a decellularized matrix scaffold loaded with SDF-1 alpha, which continuously releases SDF-1 alpha to recruit endogenous stem cells in the later repair stage, and promotes functional regeneration of the endometrium. The application utilizes the time sequence microenvironment changes of endometrial repair, and realizes the cascade regulation of 'anti-inflammation first, metabolic repair second, and regeneration promotion third'. Animal experiments show that the system can significantly restore the reproductive function of the injured endometrium and improve the number of offspring.

The invention provides injectable growth factor hydrogel as well as a preparation method and application thereof, and relates to the technical field of medical materials. The injectable growth factor hydrogel is developed by taking glycyrrhizic acid (GA) and dopamine modified hyaluronic acid (HA-DA) as a biological matrix material and taking baicalein A (OA) and a platelet-derived growth factor (PDGF-BB) as bioactive functional components. The invention aims to solve the technical problems of non-lasting growth factor release, difficult hydrogel injection, single endometrial repair effect and the like in the existing treatment method, can promote tissue repair and fertility recovery, and has a remarkable clinical application prospect.

The invention belongs to the field of medicines, and particularly provides application of mesenchymal stem cells in a medicine for treating thin endometrium and uterine cavity adhesion. The invention further provides a PDGF-BB monoclonal antibody, and the antibody can be specifically combined with PDGF-BB so as to inhibit corresponding protein activity. After the monoclonal antibody is used independently or in combination with stem cells, the effects of effectively promoting gland growth and reducing the fibrosis degree can be achieved, which shows that the monoclonal antibody can be used independently or in combination with the stem cells for treating thin endometrium and uterine cavity adhesion, and the application prospect is wide.

The invention relates to a capsule for treating gynecological diseases and a preparation method thereof. The capsule is prepared from polygonum cuspidatum, sargentgloryvine stem, coptis chinensis, rhizoma bletillae, radix gentianae, dragon's blood, cuttlebone, rheum officinale, aloe, catechu, astragalus membranaceus, chloramphenicol and purified water. The capsule is prepared through a powder preparation process, a conventional vaginal medicine filling method is adopted for administration, the capsule is dissolved by virtue of vaginal secretion to release medicine, and the medicine acts on the vagina, the cervix uteri and the endometrium and directly reaches a focus, so that the effects of diminishing inflammation, sterilizing, resisting viruses, diminishing swelling, removing stasis and repairing mucosa are achieved; the traditional Chinese medicine composition can be used for treating colpitis mycotica, infectious microbe vaginitis, cervical erosion, cervicitis, HPV infection and endometritis. The capsule is a proved recipe inherited by several generations, has a good curative effect, takes traditional Chinese medicines as main raw materials, is added with a small amount of western medicines, has the advantages of benefiting and eliminating disadvantages, has no side effect, no preservative and no irritation, has an effective rate of 99% and a cure rate of 98%, and is worthy of popularization.

The application provides an evaluation method of an endometrial immune microenvironment, which evaluates the health condition of the endometrium through the aggregation state of immune cells. Compared with the prior art, the application has the following advantages: the application provides an evaluation method of an endometrial immune microenvironment and application thereof, and through the statistics of the proportion of immune cell aggregation foci on the endometrium, the microenvironment of the endometrium can be efficiently and accurately evaluated, and thus the early judgment of endometrium-related diseases such as endometrial chronic inflammation and pregnancy outcome can be effectively improved.

The invention relates to a traditional Chinese medicine composition for improving hypomenorrhea and application thereof, and belongs to the technical field of biological medicine. The herba leonuri, the anisodamine and the Dingkundan are combined to form the composition, efficacy complementation can be achieved, it is guaranteed that the medicine takes effect quickly, the long-acting treatment effect is maintained, meanwhile, the side effect caused by long-term single medication is avoided, the treatment safety and tolerance are improved, the menstrual volume can be remarkably increased, endometrial blood circulation can be improved, and the curative effect is improved. The hypomenorrhea is effectively improved. The medicines in the composition are all approved compliant medicines, the source is clear, the quality is controllable, the administration scheme is simple and easy to implement, the composition is suitable for clinical popularization and application, and a novel safe, efficient and reproducible treatment method is provided for hypomenorrhea.

The current disclosure provides novel treatments for endometrial and ovarian hyperproliferative disorders, such as endometriosis, certain cancers, and benign hyperproliferation in female reproductive tissues. According, aspects of the disclosure relate to a method for treating endometrial or ovarian hyperproliferative disorders in a subject, the method comprising administration of a modulator of LMTK3 to the subject. The disclosure further describes nucleic acids encoding peptides of the disclosure and host cells and compositions comprising the nucleic acids or peptides of the disclosure.

The invention discloses a mare follicle development noninvasive real-time monitoring method and system based on multi-parameter fusion, and relates to the crossing field of animal breeding technology and medical imaging technology. The method comprises the following steps: transmitting ultrasonic waves to mare ovarian tissues through a monitoring assembly, receiving reflection echoes, generating two-dimensional / three-dimensional ultrasonic image data of ovarian follicles and endometrium, and collecting temperature data and light intensity time sequence data; the received ultrasonic image data, temperature data and light intensity time sequence data are preprocessed respectively, and follicle parameters, endometrial parameters, pelvic cavity temperature parameters and ovarian peripheral tissue blood flow related parameters are obtained; and constructing a multi-parameter fusion model, inputting the obtained follicle parameters, endometrial parameters, pelvic cavity temperature parameters and blood flow related parameters into the model, carrying out feature fusion by adopting a deep learning architecture, and outputting a mare follicle development stage evaluation result and an ovulation time prediction value. According to the invention, injury, infection and stress risks caused by invasive operation can be avoided, and long-term multi-time monitoring can be realized.