15 results about "Basic research" patented technology

This invention relates to a method for constructing a mouse model of autosomal dominant polycystic kidney disease and its applications, particularly to a method for preparing a non-human animal model carrying a nonsense mutation in the Pkd1 gene c.616_618GAG>TAG based on CRISPR / Cas9 gene editing technology. The method involves co-injecting mouse zygotes with gRNA targeting exon 5 of the Pkd1 gene, homologous recombinant donor oligonucleotides containing the c.616_618GAG>TAG mutation, and Cas9 nuclease. Mutant mice are obtained via embryo transfer. The mutation site is verified by PCR combined with sequencing, and a stable mutant line is established through breeding. This invention also includes the application of this model animal as a research tool for autosomal dominant polycystic kidney disease, particularly in the areas of pathogenesis analysis, drug screening, and therapeutic target validation. This model can stably simulate the typical clinical manifestations and pathological features of human autosomal dominant polycystic kidney disease, providing an important tool for basic research and translational medicine of this disease.

This invention relates to the fields of basic research in cognitive neuroscience, large-scale artificial intelligence model architecture, brain-computer interfaces, and educational cognitive assessment. Specifically, it relates to a unified four-dimensional discrete-time spatiotemporal modeling method for basic neural information elements of the brain (neural impulses, neuronal cluster firing, and sensory neural signals). This method is based on a homologous and unified underlying theoretical framework and is an engineering embodiment of a unified four-dimensional discrete-time spatiotemporal cognitive system. It shares a completely homologous, isomorphic, and unified mathematical framework with the inventor's previously submitted "A Token Tagging Method and Cognitive System for Input Data of a Large Language Model." The only difference is that the AI ​​patent processes computational information elements (Tokens), while this patent processes biological information elements (neural impulses, sensory signals, and neuronal firing events). Together, they constitute a unified underlying architecture covering biological cognition and artificial intelligence.

The application discloses an antibody capable of specifically recognizing acetylation of ribosome biogenesis factor 1 protein at the 1147th position, and a preparation method and application thereof. According to the protein sequence and modification type of ribosome biogenesis factor 1, two modified antigen polypeptides A and B are designed. It is detected that the antibody obtained by immunizing animals by using the two polypeptides has high specificity and affinity, and can accurately recognize the acetylation modification form of the 1147th position of BMS1, and avoid recognizing the unmodified form or other modification sites. The antibody is suitable for various immunological detection methods, has high repeatability, low background, has mass production potential, can be industrialized, and is not only used for evaluating the cisplatin resistance in esophageal cancer treatment, but also used for various scenes such as basic research, disease mechanism analysis, biomarker development, drug target screening, and has a wide application range.

This application discloses a 5K SNP liquid phase chip for *Armillaria galbana* and its application, relating to the field of biotechnology. The *Armillaria galbana* 5K SNP liquid phase chip includes probes for capturing a combination of *Armillaria galbana* SNP molecular markers; wherein the combination of *Armillaria galbana* SNP molecular markers includes 5510 SNP molecular markers, and the physical locations of the 5510 SNP molecular markers are based on *Armillaria galbana*. French Armillaria The site information, determined by genome sequence alignment, is shown in Table 1 of the specification. This invention provides the first SNP liquid-phase chip for Armillaria mellea, filling the gap in products applicable to molecular-assisted breeding and genome-wide selection breeding of Armillaria mellea, and providing an effective tool for accelerating basic research and new variety creation in Armillaria mellea.

The present application is suitable for the field of geographic information processing and spatial data mining technology, and provides a kind of geographic feature causal mining method considering topological neighborhood, comprising the following steps: obtaining the data of multiple types of geographic features in the target area, and pre-processing, obtaining the geographic feature set containing spatial position coordinates and category attributes;Based on the geographic feature set, the target area is divided into research units, and a plurality of basic research units are obtained, and based on the category attribute of the geographic feature, the spatial distribution of different category geographic features in each basic research unit is counted, and the distribution characteristic value is obtained.The quantitative identification of the causal action direction and the causal action intensity between geographic features can be realized;The structural connection between geographic features can be effectively described, the influence of pseudo correlation is reduced, and the accuracy and stability of causal relationship identification are improved.

This invention discloses a device and method for creating a mouse model of chronic pancreatitis by impact, belonging to the technical field of animal experimental equipment. The device includes a vibration generator, a vibration rod, and a vibration head, and can also be equipped with an outer shell, levers, and a buffer sleeve. The vibration generator drives the vibration rod to generate high-frequency reciprocating vibrations in the vibration head. The vibration head is a miniature rigid structure with various end shapes such as spherical, spoon-shaped, and forceps-shaped. The outer shell enables tissue dissection, pancreatic clamping, and axial extension and retraction of the vibration head. The buffer sleeve blocks vibration transmission. The modeling method involves pre-setting the device parameters, exposing the pancreas through levers after abdominal incision, allowing the vibration head to contact the target area and initiate high-frequency vibration to complete the modeling. This device achieves precise targeted stimulation of the mouse pancreas, with controllable damage intensity, avoiding collateral damage to surrounding organs. It is simple to operate, has high model repeatability, and can construct mouse models of chronic pancreatitis of different degrees, making it suitable for basic research on pancreatic diseases.

The application discloses a molecular marker primer for a narrow cod and application thereof, and relates to the technical field of molecular biology markers. The primer combination is composed of three pairs of primers, namely, GC6:TC14, GC8:GT15 and GC9:TG9, and the sequences of the primers are shown as SEQ ID NO. 1-6. The application develops, for the first time, the STR molecular marker primer combination specially used for sea area source identification of the narrow cod. Compared with the microsatellite marker used only for the population genetics basic research of the narrow cod in the prior art, the primer combination is optimized through multi-stage screening, can effectively distinguish the narrow cod samples from the Okhotsk Sea FAO 61 sea area and the Bering Sea FAO 67 sea area, and fills the technical blank in the sea area tracing field of the cross-border trade of the narrow cod.

This invention belongs to the technical field of basic uranium geological research, and specifically discloses a method for predicting and evaluating sandstone-type uranium deposits in environments with relatively strong tectonic structures. The method includes: step (1) collection and organization of regional geological data; step (2) analysis of basin tectonic geological background and basin tectonic types; step (3) study of typical deposits; step (4) map compilation and study of regional uranium mineralization regularities; and step (5) extraction of prediction elements and prospective prediction. This invention, through the collection and organization of geological data from a geological region, combined with limited regional geological work, fully analyzes the tectonic geological background and metallogenic geological conditions; through the study of typical deposits, it identifies the target layers and key ore-controlling elements for prospecting, summarizes regional uranium mineralization regularities, extracts prediction elements, predicts and delineates favorable mineralization zones, and provides a basis for strategic uranium ore selection and medium- to long-term uranium geological exploration deployment.

The application relates to the technical field of AI chips, and provides a terminal and an implementation method for hotspot analysis of basic scientific research institutions based on edge computing and light-weight AI. The terminal comprises a vector set acquisition system, a data packet generation system and a differential display system. The method comprises the following steps: a layered feature stripping of a light-weight AI model architecture is utilized to decompose scientific research literature content into multi-dimensional feature tuples such as semantic cores, methodology labels and data reference networks; a set of standardized and light-weight feature vectors are obtained; a preliminary hotspot label with an intensity identifier exceeding an adaptive threshold and core hotspot data associated with the preliminary hotspot label are marked out to generate a core hotspot data packet; a macro skeleton of a core hotspot atlas is presented on a terminal interface; differential highlighting is performed according to the intensity identifier in the core hotspot data packet; and deep detail information corresponding to a hotspot node is dynamically loaded and rendered. The application presents a macro hotspot trend and supports user interaction exploration to obtain deep information.

The present application relates to the technical field of insulation film, especially to the field of IPC G03F, more particularly to a preparation method of a photosensitive integrated circuit stacked insulation film. The present application is based on the actual market demand, and scientifically designs the formula of the stacked insulation film material by combining basic research with industrial development, so as to realize the manufacturing of the fine line laminated adhesive film with low thermal expansion coefficient, high frequency low dielectric, low loss, high bonding force. The material has good performance in insulation, mechanics, heat conduction and the like, and does not depend on the expensive precision drilling equipment imported, and these performances enable it to replace ABF in the electronic field such as microchip integrated insulation technology, and have wide application prospect.

This invention provides an in vitro packaging method for ring virus pseudovirus particles, belonging to the field of molecular biology technology. The method involves eukaryotic expression of a ring virus capsid expression plasmid. Ring virus pseudovirus particles can serve as a safe and efficient novel viral vector for gene therapy and basic research. The technical method of this invention will promote basic research on ring viruses and related vector-based applications.

This invention relates to the field of biotechnology, and particularly to primers and methods for whole-genome sequencing of African swine fever virus (ASFV) based on next-generation sequencing platforms and targeted enrichment. This invention discloses a primer set and method for whole-genome sequencing of ASFV based on next-generation sequencing platforms and targeted enrichment, comprising 32 pairs of specific targeted amplification primers. These primers exhibit high specificity and sensitivity, fully leveraging the advantages of next-generation sequencing—high throughput, low cost, high accuracy, and mature technology—to accurately and completely obtain the entire ASFV genome sequence. This invention can obtain complete genetic information of ASFV, identify and diagnose infections of different ASFV genotypes, and, by analyzing the genetic differences among different strains, determine the geographical origin and transmission dynamics of circulating strains, tracing the evolution of recombinant strains. It provides resources for basic research on virus-host interactions and drug resistance, and offers a theoretical basis for developing long-term prevention and control strategies. It also promotes innovation in diagnostic methods.

This invention belongs to the field of analytical chemistry and biomedical detection, and discloses a method for detecting nucleotides in biological samples based on liquid chromatography-mass spectrometry (LC-MS). The method includes: pretreatment of the biological sample by homogenization with a weakly basic ammonium acetate buffer, protein precipitation with organic solvent, vacuum concentration, and reconstitution; separation using an alkali-resistant C18 column and a weakly basic mobile phase with gradient elution; and detection by multiple reaction monitoring (MRM) in negative ion mode using an electrospray ionization source. This invention can simultaneously detect more than 40 target substances, covering bases, nucleosides, deoxynucleosides, and their phosphorylated forms and modified metabolites, with a limit of quantitation at the ng / pg / mL level. It exhibits strong resistance to matrix interference and excellent chromatographic separation. This method is rapid, highly sensitive, and stable, suitable for high-throughput and precise detection of nucleotides in biological samples, providing a powerful tool for disease biomarker screening and related basic research.

This invention discloses a barium titanate-based piezoelectric ceramic with high temperature stability and its preparation method. The ceramic has the following characteristics: Ba 1‑x Ca x Ti 1‑y Zr y O3, 0.17≤x≤0.19, 0.11≤y≤0.13. The ceramic uses BaTiO3 as the basic research system. By replacing some barium ions with calcium ions and some titanium ions with zirconium ions, the phase transition temperature of barium titanate is adjusted, so that it maintains stable piezoelectric properties over a wider temperature range, i.e., it has high temperature stability. Furthermore, by doping with calcium and zirconium sources to change the morphology and distribution of electric domains, the piezoelectric coefficient of the ceramic is increased.

The application discloses a molecular marker primer for STR of Trachurus japonicus and application thereof, and relates to the technical field of molecular biology markers. The primer combination is composed of three pairs of primers, namely LS4:CA8, LS6:AC16 and LS8:AC16, and the sequences of the primers are shown as SEQ ID NO. 1-6. The application develops, for the first time, the primer combination of the STR molecular marker specially used for the identification of the sea source of Trachurus japonicus. Compared with the microsatellite marker used only for the basic research of the population genetics of Trachurus japonicus in the prior art, the primer combination of the application is optimized through multi-stage screening, can effectively distinguish the Trachurus japonicus samples from the Indian Ocean FAO 57.1 sea area and the Arabian Sea FAO 51.4 sea area, and fills the technical blank in the field of the sea source tracing of the cross-border trade of Trachurus japonicus.