665 results about "Basic research" patented technology

The present invention relates to a new method for reversible staining and functional isolation or characterization of cells, e.g. antigen-specific T cells. With this technique, the original functional status of cells can be substantially maintained after their identification and purification. Thus, this new method is of broad benefit for basic research and clinical applications.

The present invention provides methods and routines for developing and optimizing nucleic acid detection assays for use in basic research, clinical research, and for the development of clinical detection assays. In particular, the present invention provides methods for designing oligonucleotide primers to be used in multiplex amplification reactions. The present invention also provides methods to optimize multiplex amplification reactions. The present invention also provides methods for combined target and signal generation assays.

The present invention relates to a new method for reversible staining and functional isolation or characterization of cells, e.g. antigen-specific T cells. With this technique, the original functional status of cells can be substantially maintained after their identification and purification. Thus, this new method is of broad benefit for basic research and clinical applications.

The present invention relates to systems and methods for biomedical drug research, addressing major molecular, cell biological and biochemical information management issues within drug discovery and basic biomedical science. The invention allows scientists to enter biological, chemical, and / or molecular data into a central database, analyze the data entries according to entry attributes, and graphically view the results. A group of web-enabled researchers can enter, share and analyze molecular and cellular data and information from the resources using standardized vocabularies and ontologies. This application describes in detail components of the databasing system, including but not limited to annotation modules, reference managers, advanced search algorithms, ontology browsers, molecular network builders, and text processing scripts. Ultimately, the information gathered, viewed, and analyzed by this relational databasing system is relevant to research ranging from basic researchers to advanced research in applied technologies within pharmaceutical development and biotech fields.

The present invention relates to a reporter construct and method for identifying or enriching the cells, wherein a specific endogenous nucleotide sequence is cleaved by a specific nuclease or modified by such cleavage; a host cell comprising the reporter construct; and a system for monitoring a nuclease activity. The reporter system of the present invention is simple and non-invasive, and allows for an efficient enrichment of the gene-modified cells. Therefore, the present invention will promote the application of a nuclease in the field of gene therapy and genetic engineering as well as basic research.

The present invention provides methods and routines for developing and optimizing nucleic acid detection assays for use in basic research, clinical research, and for the development of clinical detection assays. In particular, the present invention provides methods for designing oligonucleotide primers to be used in multiplex amplification reactions. The present invention also provides methods to optimize multiplex amplification reactions. The present invention also provides methods for combined target and signal generation assays.

The invention relates to a mouse monoclonal antibody of anti-human carcino-embryonic antigen (CEA) resisting colorectal neoplasm activity, prepared by a biological technology. The method comprise a method for detecting CEA family protein by utilizing the antigen-combining capacity specificity of the mouse monoclonal antibody CC4 of anti-human CEA and a neoplasm treatment method by utilizing the colorectal neoplasm activity. The antibody can be used for specifically recognizing the human CEA at molecular, cellular and texture levels and recognizing a special antigen epiposition widely distributed in the CEA family protein. The CC4 is specifically combined with colorectal neoplasm texture at the texture level and presents stronger capabilities of restraining neoplasm growth, neoplasm metastasis, neoplasm invasion in an animal model and at the cellular level. The antibody and detection and treatment methods based on the antibody can become an effective neoplasm diagnosis and treatment tool in basal research or clinical application.

The invention provides a high-water pressure, high-stress and automatic-mining mine water disaster comprehensive analog system and a test method, belonging to the technical field of basic research of water prevention and treatment in a mine. The invention mainly relates to a mine water damage comprehensive analog system which is good in leakproofness, and can simulate the mining, reflect the mutual function between a rock mass at a top plate and a base plate and the water, and obtain the change regularity of solid and liquid physical mechanics parameters under the conditions of high water pressure and high vertical stress. By adopting a cylindrical closed test chamber and an automatic mining analog device in the chamber, the system allows the highest water pressure at 3MPa, applies the maximum vertical load at 3MPa, has the test diameter at 1.4m, and has the sample assembly height at 1.4m. The system comprises a model test chamber, a stable high-water pressure (flow) water supply and control device, a mining simulating and controlling device, a multiple interference flow observing device and an assembly observing subsystem of pressure, stress, temperature and PH value. The invention further establishes a test method for automatically simulating the mining of the dynamic sudden water inflow by the top plate and the base plate of the mine under the conditions of high water pressure and high stress and the real-time monitoring.

The invention discloses a laser capable of simultaneously outputting nanosecond pulses and pico-second pulses. The laser comprises a signal source, a signal isolation system and an amplifier. According to the invention, with an electric light control technology of combining an extra-cavity Pockels cell and intra-cavity Pockels cell, a regenerative amplification process is realized in case of signal light importing so as to obtain high-energy pico-second laser output, and cavity empty process is also realized in case of no signal light importing so as to obtain nanosecond pulse output; and thefree combination of the two processes can be realized according to practical application demand, and as a result, free combination of numbers of the nanosecond pulses and the pico-second pulses in a single laser is realized and is output in an alternative way. The laser provided by the invention has an important application in high-precision high-efficiency fine processing field, as well as basicresearch fields such as plasma excitation, excited state control, chemical reaction control, special radiation excitation and the like.

An encephalic electrode individualization locating method based on multimode medical image data fusion relates to the fields of medical instruments and medical image processing and neuroimaging. By means of multimode medical image data including magnetic resonance images, X-ray images and intranperative optical photos, the space position relation of an encephalic electrode and a brain tissue structure is comprehensively measured, a relation between the position of the encephalic electrode and a brain anatomical structure is established, and accurate and fast individualization locating of the encephalic electrode is achieved. The encephalic electrode individualization locating method based on multimode medical image data fusion can be used in the fields of cognitive neuroscience basic research, clinic neurosciences and the like, provide accurate brain space position information for an encephalic electroencephalogram signal, and enhance application value in the aspect of electrophysiological basis research.

The invention discloses a residential building indoor environment monitoring and health grade evaluation Internet of Things system, and belongs to the fields of the residential building indoor environment detection technology and the information communication technology. The residential building indoor environment monitoring and health grade evaluation Internet of Things system is characterized in that indoor various-function room environment parameters are acquired in real time by utilizing data acquisition modules, and influence weights and health risk grades of the various indoor environment parameters on human health are comprehensively analyzed; and an expert opinion on improving of a corresponding environment condition and an expert strategy for improving the corresponding environment condition are given. An Internet of Things real-time data monitoring system is built at the same time, data can be timely transmitted to a user display terminal and a remote central service system, and the data can be taken as basic research data and a decision basis of a relevant government department. The residential building indoor environment monitoring and health grade evaluation Internet of Things system is advantageous in that the problem that the fact that the indoor environment is represented by a single parameter is unscientific is solved, real-time monitoring of the residential environment indoor comprehensive evaluation parameters and user mobile terminal contrast result synchronous searching can be achieved at the same time, and an suggestion for effective improvement of the environment and a measure for effectively improving the environment are obtained; and the residential building indoor environment monitoring and health grade evaluation Internet of Things system has the advantages of the wide application aspect and the high popularizing rate.

The invention discloses a semi-physical real-time simulation platform for a wind generating set and belongs to the technical field of clean energy. The simulation platform is mainly composed of an upper computer monitoring interface, a wind generating set simulation system, a controller simulation system, a current transformer controller and a variable pitch system. An upper computer is respectively connected with the wind generating set simulation system and the controller simulation system through Ethernet. The simulation platform can quickly develop and verify a control algorithm, can simulate operation characteristics of a real wind generating set, enables a controller to carry out comprehensive loop test before true machine set test, saves test cost, improves operation safety, can control the output power of a fan, stabilizes power grid fluctuation and provides a basic research platform for optimal operation of a wind power plant.

The present invention provides methods and routines for developing and optimizing nucleic acid detection assays for use in basic research, clinical research, and for the development of clinical detection assays. In particular, the present invention provides methods for designing oligonucleotide primers to be used in multiplex amplification reactions. The present invention also provides methods to optimize multiplex amplification reactions.

The invention aims at providing a novel full-thickness biological cornea used for transplantation. The full-thickness biological cornea takes an animal cornea acellular matrix as a carrier, and the acellular matrix comprises animal cornea matrix cells, epithelial cells and endothelial cells which are cultured and augmented in vitro. The cornea is characterized in that the xenogenic corneal acellular matrix prepared by a biochemical method can be used as a good carrier for in vitro constructing the biological cornea in the aspects of shape, structure and biological compatibility, the matrix cells, epithelial cells and endothelial cells of the cornea are planted respectively, and dynamically cultured in the simulated in vivo environment of a biological reactor, so that the full-thickness biological cornea with nearly normal tissue structure and characteristics can be constructed in vitro, and the biological cornea can be used to simulate the physiological cornea for fundamental research on physiology, pathology and pharmacology; moreover, the biological cornea can also be directly used as the donator for corneal transplantation.

The invention discloses a method for extracting high-purity humic acid from low-rank coal. In the invention, abundant low-quality peat, lignite, weathered coal and the like in china are used as raw materials; and processes such as repeated base-dissolving acidification, centrifugal separation, mixed acid treatment, semi-permeable membrane dialysis, freeze drying and the like are carried out to separate and obtain a high-purity humic acid product with a humic acid content not lower than 97%. Compared with the traditional base-dissolving acidification method and the existing high-purity humic acid preparation method, the method has the advantages of low processing cost, simplicity and convenience in technological operation, high product purity, low impurity element content and the like. The high-purity humic acid prepared by the method can be applied to cathode plate expanding agents of lead storage batteries, medical products and the like, and can be used as a chemical reagent for related basic researches of humic acid substances and an important raw material for synthesis of organic polymers.

The present invention discloses a method which adopts a single specific capture agent for quantitative detection of the analyte in the biological sample, which is named as the analyte labeling and inversion recapture method, ALIRA for short, which is an improved detection method which is based on the principles of the SALRA but adopts different capture means. The method comprises: capturing the analyte on the capture device, and forming capture agent-analyte complex; labeling the complex with a labeling agent; eluting the labeled analyte from the complex; recapturing the analyte through the combination of the second recapture agent on the detection device and the labeling molecules; and adding specific detection agent, compounding the specific detection agent and the analyte, and determining the concentration of the analyte according through detecting the signals which are generated by the report modules which are carried by the specific detection agent. The invention has the advantage that the adoption of a specific detection agent can sensitively and simply complete the quantitative detection of the analyte, and the invention has excellent application prospects in the application in the disease diagnosis, medical evaluation, new drug development, protein micro-array and the like, and in the field of basic research.

The invention generally relates to the field of immunochemistry including antibody therapy, diagnostics, and basic research and specifically relates to the area of selecting affinity molecules such as natural antibodies, including artificial antibodies, antibody mimics, and aptamers. The invention relates particularly to a method of selecting affinity molecules using a homogeneous noncompetitive assay in a high throughput process.

The identification of pre-defined mutations expected to be present in a minor fraction of a cell population is important for a variety of basic research and clinical applications. The exponential, analog nature of the polymerase chain reaction is transformed into a linear, digital signal suitable for this purpose. Single molecules can be isolated by dilution and individually amplified; each product is then separately analyzed for the presence of pre-defined mutations. The process provides a reliable and quantitative measure of the proportion of variant sequences within a DNA sample.

The present invention relates to degradation-stabilised, biocompatible collagen matrices which are distinguished in particular by the fact that they contain soluble collagen and peptide constituents, to processes for the preparation of such collagen matrices, which processes include in particular chemical crosslinking with an epoxy-functional crosslinking agent, and to the use of the collagen matrices according to the invention as a cosmetic or pharmaceutical agent, in particular for topical use, and as a wound treatment agent, as an implant or as a haemostatic agent in humans or animals, and as a scaffold for cell population in the biotechnology, basic research and tissue engineering field.

The invention relates to a group of anti-human CD146 molecules which are developed by utilizing the biological technology and an established high-sensitive sandwich ELISA method for detecting the solubility CD146. The invention includes anti-human CD146 mouse monoclonal antibodies of AA1, AA2, AA3, AA4, AA5 and AA7, and the method of utilizing the combination of the antibodies and the sandwich ELISA specificity for detecting the solubility CD146. The group of antibodies can identify the human source CD146 on the molecular, the cellular and the tissue levels, which can be divided into two categories based on the identified different epitopes. The sandwich ELISA method which is combined by the antibody AA1 and another strain of anti-human CD146 mouse monoclonal antibody AA98 can detect the solubility CD146 at each milliliter nano-gram level. The antibodies and the detection means can become the effective detection or diagnosis tools and method in the basic research or the clinical application and provide good carriers for the targeted therapy of the CD146-related diseases at the same time.

This invention related to methods and assays for screening for, identifying, and predicting the severity and clinical manifestations of systemic lupus erythematosus (SLE). Specifically, this invention provides various biomarkers for the prediction of flares of the disease both in number and severity, as well as clinical manifestations of the disease, and methods of using these biomarkers to correctly subclassify patients with this disease, and prescribe appropriate treatment. The invention also provides for biomarkers of lupus disease activity, i.e., flares, as well as biomarkers for the prediction of future flares, and methods of using these biomarkers. The invention also provides, in these biomarkers, targets and methods for drug development and basic research for SLE.

The invention discloses a serum-free complete medium for mesenchymal stem cell, consisting of a basal medium and an additive component, wherein the basal medium is DMEM / F12 (dulbecco modified eagle medium), and the additive component consists of recombinant human insulin, human serum albumin, human transferrin, cholesterol, sodium selenite, ferric nitrate and glucan. The complete medium is free from serum, so that the defects that the serum-containing medium is unstable in serum batches, high in cell toxicity, abundant in heterologous protein and the like can be greatly avoided, and a good tool can be provided for the basic research and the clinic treatment of the mesenchymal stem cell; and the invention further achieves the effect which is approximate or equal to the serum-containing complete medium and the other serum-free complete mediums in the aspect of cell proliferation, so that the long-term subcultring of the cell can be supported.

The invention relates to the automatic generating method of the molecular mechanic field's parameter, including the method to generate automatically the parameter of the molecular mechanic that is required in molecular simulation, and it can be used for basic research such as the materials science, life sciences, medication science, and chemical industry and so on. The main content of the invention are: searching the only molecular-model collection which can be used for generating the molecular field's parameter for the goal molecular; carrying quantum-mechanical calculation on the molecular-model collection to get the reference data that can be used for fitting the molecular mechanics field; fitting automatically a lot of nonlinear data; verifying automatically the fitting results; storing and managing the data and fitting results referred above with database. The invention can solve the bottle-neck problem that lacking perfect and precise force-field in the use of the molecular simulation technique abroad at present. The invention provides a new systematic method which can deduce molecular force field quickly, exactly and automatically.

The invention relates to a high simulation tissue engineering nerve repairing material NGCS and a preparing method thereof which is characterized in that the NGCS material comprises one, two or three raw materials of type I collagen, chitosan and gelatin. The NGCS material is made of the following raw materials by weight portions: 1-10 portions of type I collagen, 1 portion of chitosan and 1 portion of gelatin. The NGCS can be applied to both the basic research of the nerve injury repairing and the bridge repairing of clinical human spinal and peripheral nerve injuries or defects. The NGCS material is beneficial for the growth of nerve regenerated fiber and can be applied to the repairing of spinal and peripheral nerve injuries.

The invention provides a method and its kit for quantitatively detecting a specific analyte with a single capturing agent. The quantitative detection of a specific analyte with a single capturing agent comprises: firstly combining the tested analyte with a solid phase capturing agent, then labeling analyte which has been trapped by the capturing agent with a report molecule; secondly eluting the labeled analyte from the complex, recombining the tested analyte with a new solid phase capturing agent, and ascertaining the content of analyte by detecting the report molecule's label signal. The kit of the invention comprises a capturing device, a detecting device, a report molecule for labeling and an analysis substance eluate. The advantages of the invention are the need of one single capturing agent, the capability of detecting for many analytes which can't be tested at present, wide application, high sensibility and low noise. The invention can be applied to diagnosis, medical expertise, new medicine development, application of protein micro array and chip, and fundamental research.

The invention provides a Period-divided and reason-divided prediction evaluation method of a natural fracture in a gas reservoir. The specific implementation scheme comprises a step of obtaining a fracture filling sample, a step of carrying out period-divided matched analysis on the sample, a step of carrying out a rock sound emission experiment to simulate and recovery the memory period of a rock fracture, a step of determining a cause type, a step of determining the formation period and reason type of a natural fracture and dividing the natural fracture system in the gas reservoir into a plurality of natural fracture sub systems with single periods and single reasons, a step of carrying out comprehensive superposition on the prediction result of each natural fracture sub system to form a prediction result, and a step of carrying out comparison analysis on the obtained prediction result and fracture development. By employing the above technical scheme, the multiple-period and multiple-reason complex natural fracture network system is divided into multiple natural fracture sub systems with single periods and single reasons to be a basic research object through period separation and reason type determination, and the precision of gas reservoir natural fracture prediction is improved.

Provided is a method for calculating dynamic characteristics of a contactor based on a piecewise linear kinetic equation, and belongs to the technical field of basic research design of general characteristics of contactors. The method comprises the steps that according to structural features of the contactor, segmentation is conducted on a contactor action process, plane collision and a continuouscollision force model with energy loss considered are used for being equivalent to a collision process, by means of the multi-body system kinematics based on a restrain method and a dynamic method, studying of the collision bounce situation of a contactor mechanical system under the action of external force is completed, and finally, dynamic characteristic calculation of the contactor with collision bounce considered is completed in combination of a coupling calculation method. The method has the advantages that the time-dependent situation of electromagnetic parameters such as the coil current and electromagnetic force in a working process of the contactor can be obtained, the electromagnetic dynamic characteristics of the contactor can be reflected more accurately, and errors are controlled within 5%; the bounce situation of an armature and a moving contact of the contactor can be reflected more really, and errors are controlled within 5%.

The invention discloses a simulation evaluation method for a configuration scheme of a safe automatic device of a transmitting-end power grid high-frequency cutting machine. The method comprises the following steps of: establishing a transmitting-end power grid frequency simulation data model comprising important components related with frequency control of a machine set side and a power grid side; setting basic research operating points of the electric power outgoing level of a transmitting-end electric network; supposing that the safe automatic device of a direct current latch-up linkage cutting machine is correctly operated, calculating two types of outgoing channel fault forms of the transmitting-end power grid for each basic research operating point one by one to obtain a simulation result of the transmitting-end electric network; supposing that the safe automatic device of the direct current latch-up linkage cutting machine fails to operate, calculating the two types of outgoing channel fault forms of the transmitting-end power grid for each basic research operating point one by one to obtain a simulation result of the transmitting-end electric network; and according to the simulation results obtained in the steps C and D, evaluating whether the effect of the configuration scheme of the safe automatic device of the high-frequency cutting machine is good. The method has the advantages of fast and accurate evaluation, comprehensive factor consideration and the like, is easy and convenient to operate, and is high in practical value.

The invention relates to the field of micromachining technology and tissue engineering technology, and particularly relates to a microfluidic chip and method for establishing an in-vitro co-culture model of three kinds of cells. The microfluidic chip is used for establishing a co-culture model of three kinds of cells; different cells are located in different areas through connected micro-channels in the microfluidic chip; and two kinds of cells can conduct cell communication through the connected micro-channels. Therefore, through the scheme, a new model capable of connecting astrocyte as a middle bridge in a neurovascular unit with the neuron and microvascular endothelial cell in a three-dimensional or plane space is established in vitro, a neurovascular network in the brain can be reproduced in vitro more scientifically and really, and a better platform is provided for clinical basic research on the central nervous system diseases and particularly the blood vessels of brain; and meanwhile, as an emerging biochip, the microfluidic chip is used for establishing the neurovascular in vitro, and thus the application range of the microfluidic chip is further widened.