662 results about "Basic research" patented technology

The present invention relates to a new method for reversible staining and functional isolation or characterization of cells, e.g. antigen-specific T cells. With this technique, the original functional status of cells can be substantially maintained after their identification and purification. Thus, this new method is of broad benefit for basic research and clinical applications.

The present invention provides methods and routines for developing and optimizing nucleic acid detection assays for use in basic research, clinical research, and for the development of clinical detection assays. In particular, the present invention provides methods for designing oligonucleotide primers to be used in multiplex amplification reactions. The present invention also provides methods to optimize multiplex amplification reactions. The present invention also provides methods for combined target and signal generation assays.

The present invention relates to systems and methods for biomedical drug research, addressing major molecular, cell biological and biochemical information management issues within drug discovery and basic biomedical science. The invention allows scientists to enter biological, chemical, and/or molecular data into a central database, analyze the data entries according to entry attributes, and graphically view the results. A group of web-enabled researchers can enter, share and analyze molecular and cellular data and information from the resources using standardized vocabularies and ontologies. This application describes in detail components of the databasing system, including but not limited to annotation modules, reference managers, advanced search algorithms, ontology browsers, molecular network builders, and text processing scripts. Ultimately, the information gathered, viewed, and analyzed by this relational databasing system is relevant to research ranging from basic researchers to advanced research in applied technologies within pharmaceutical development and biotech fields.

The invention relates to a mouse monoclonal antibody of anti-human carcino-embryonic antigen (CEA) resisting colorectal neoplasm activity, prepared by a biological technology. The method comprise a method for detecting CEA family protein by utilizing the antigen-combining capacity specificity of the mouse monoclonal antibody CC4 of anti-human CEA and a neoplasm treatment method by utilizing the colorectal neoplasm activity. The antibody can be used for specifically recognizing the human CEA at molecular, cellular and texture levels and recognizing a special antigen epiposition widely distributed in the CEA family protein. The CC4 is specifically combined with colorectal neoplasm texture at the texture level and presents stronger capabilities of restraining neoplasm growth, neoplasm metastasis, neoplasm invasion in an animal model and at the cellular level. The antibody and detection and treatment methods based on the antibody can become an effective neoplasm diagnosis and treatment tool in basal research or clinical application.

The invention discloses a laser capable of simultaneously outputting nanosecond pulses and pico-second pulses. The laser comprises a signal source, a signal isolation system and an amplifier. According to the invention, with an electric light control technology of combining an extra-cavity Pockels cell and intra-cavity Pockels cell, a regenerative amplification process is realized in case of signal light importing so as to obtain high-energy pico-second laser output, and cavity empty process is also realized in case of no signal light importing so as to obtain nanosecond pulse output; and thefree combination of the two processes can be realized according to practical application demand, and as a result, free combination of numbers of the nanosecond pulses and the pico-second pulses in a single laser is realized and is output in an alternative way. The laser provided by the invention has an important application in high-precision high-efficiency fine processing field, as well as basicresearch fields such as plasma excitation, excited state control, chemical reaction control, special radiation excitation and the like.

An encephalic electrode individualization locating method based on multimode medical image data fusion relates to the fields of medical instruments and medical image processing and neuroimaging. By means of multimode medical image data including magnetic resonance images, X-ray images and intranperative optical photos, the space position relation of an encephalic electrode and a brain tissue structure is comprehensively measured, a relation between the position of the encephalic electrode and a brain anatomical structure is established, and accurate and fast individualization locating of the encephalic electrode is achieved. The encephalic electrode individualization locating method based on multimode medical image data fusion can be used in the fields of cognitive neuroscience basic research, clinic neurosciences and the like, provide accurate brain space position information for an encephalic electroencephalogram signal, and enhance application value in the aspect of electrophysiological basis research.

The invention discloses a residential building indoor environment monitoring and health grade evaluation Internet of Things system, and belongs to the fields of the residential building indoor environment detection technology and the information communication technology. The residential building indoor environment monitoring and health grade evaluation Internet of Things system is characterized in that indoor various-function room environment parameters are acquired in real time by utilizing data acquisition modules, and influence weights and health risk grades of the various indoor environment parameters on human health are comprehensively analyzed; and an expert opinion on improving of a corresponding environment condition and an expert strategy for improving the corresponding environment condition are given. An Internet of Things real-time data monitoring system is built at the same time, data can be timely transmitted to a user display terminal and a remote central service system, and the data can be taken as basic research data and a decision basis of a relevant government department. The residential building indoor environment monitoring and health grade evaluation Internet of Things system is advantageous in that the problem that the fact that the indoor environment is represented by a single parameter is unscientific is solved, real-time monitoring of the residential environment indoor comprehensive evaluation parameters and user mobile terminal contrast result synchronous searching can be achieved at the same time, and an suggestion for effective improvement of the environment and a measure for effectively improving the environment are obtained; and the residential building indoor environment monitoring and health grade evaluation Internet of Things system has the advantages of the wide application aspect and the high popularizing rate.

The invention discloses a semi-physical real-time simulation platform for a wind generating set and belongs to the technical field of clean energy. The simulation platform is mainly composed of an upper computer monitoring interface, a wind generating set simulation system, a controller simulation system, a current transformer controller and a variable pitch system. An upper computer is respectively connected with the wind generating set simulation system and the controller simulation system through Ethernet. The simulation platform can quickly develop and verify a control algorithm, can simulate operation characteristics of a real wind generating set, enables a controller to carry out comprehensive loop test before true machine set test, saves test cost, improves operation safety, can control the output power of a fan, stabilizes power grid fluctuation and provides a basic research platform for optimal operation of a wind power plant.

The invention discloses a method for extracting high-purity humic acid from low-rank coal. In the invention, abundant low-quality peat, lignite, weathered coal and the like in china are used as raw materials; and processes such as repeated base-dissolving acidification, centrifugal separation, mixed acid treatment, semi-permeable membrane dialysis, freeze drying and the like are carried out to separate and obtain a high-purity humic acid product with a humic acid content not lower than 97%. Compared with the traditional base-dissolving acidification method and the existing high-purity humic acid preparation method, the method has the advantages of low processing cost, simplicity and convenience in technological operation, high product purity, low impurity element content and the like. The high-purity humic acid prepared by the method can be applied to cathode plate expanding agents of lead storage batteries, medical products and the like, and can be used as a chemical reagent for related basic researches of humic acid substances and an important raw material for synthesis of organic polymers.

The invention discloses a deep seam strip mining and filling simulation test system and method, relates to the field of preventing and treating deep rock fissure evolution and water inrush, and solves the important problem that the prior fundamental research is relatively insufficient on rock fracturing law of deep coal seam mining on high confined water, fissure evolution characteristics and inrush prevention by filling; the test system comprises a bearing box, a test model and a filling system; the test model is positioned in the bearing box and comprises a rock stratum and a coal seam positioned in the rock stratum, the rock stratum is made by laying similar material, a water confining device is arranged around the rock stratum, a plurality of sensors and a plurality of plastic balls loaded with calcium oxide particles are buried in the rock stratum, and the coal seam is simulated by arranging a plurality of loaded air bags. The test system can simulate the mining of coal seams under different ratios of extraction to reservation and different mining thickness, and can also provide the study on stress and displacement change law of roof and floor after the mining of coal seams under ratios of extraction to reservation and different mining thickness and after filling of a gob.

The identification of pre-defined mutations expected to be present in a minor fraction of a cell population is important for a variety of basic research and clinical applications. The exponential, analog nature of the polymerase chain reaction is transformed into a linear, digital signal suitable for this purpose. Single molecules can be isolated by dilution and individually amplified; each product is then separately analyzed for the presence of pre-defined mutations. The process provides a reliable and quantitative measure of the proportion of variant sequences within a DNA sample.

The invention relates to a group of anti-human CD146 molecules which are developed by utilizing the biological technology and an established high-sensitive sandwich ELISA method for detecting the solubility CD146. The invention includes anti-human CD146 mouse monoclonal antibodies of AA1, AA2, AA3, AA4, AA5 and AA7, and the method of utilizing the combination of the antibodies and the sandwich ELISA specificity for detecting the solubility CD146. The group of antibodies can identify the human source CD146 on the molecular, the cellular and the tissue levels, which can be divided into two categories based on the identified different epitopes. The sandwich ELISA method which is combined by the antibody AA1 and another strain of anti-human CD146 mouse monoclonal antibody AA98 can detect the solubility CD146 at each milliliter nano-gram level. The antibodies and the detection means can become the effective detection or diagnosis tools and method in the basic research or the clinical application and provide good carriers for the targeted therapy of the CD146-related diseases at the same time.

This invention related to methods and assays for screening for, identifying, and predicting the severity and clinical manifestations of systemic lupus erythematosus (SLE). Specifically, this invention provides various biomarkers for the prediction of flares of the disease both in number and severity, as well as clinical manifestations of the disease, and methods of using these biomarkers to correctly subclassify patients with this disease, and prescribe appropriate treatment. The invention also provides for biomarkers of lupus disease activity, i.e., flares, as well as biomarkers for the prediction of future flares, and methods of using these biomarkers. The invention also provides, in these biomarkers, targets and methods for drug development and basic research for SLE.

The invention discloses a serum-free complete medium for mesenchymal stem cell, consisting of a basal medium and an additive component, wherein the basal medium is DMEM/F12 (dulbecco modified eagle medium), and the additive component consists of recombinant human insulin, human serum albumin, human transferrin, cholesterol, sodium selenite, ferric nitrate and glucan. The complete medium is free from serum, so that the defects that the serum-containing medium is unstable in serum batches, high in cell toxicity, abundant in heterologous protein and the like can be greatly avoided, and a good tool can be provided for the basic research and the clinic treatment of the mesenchymal stem cell; and the invention further achieves the effect which is approximate or equal to the serum-containing complete medium and the other serum-free complete mediums in the aspect of cell proliferation, so that the long-term subcultring of the cell can be supported.

The invention relates to the automatic generating method of the molecular mechanic field's parameter, including the method to generate automatically the parameter of the molecular mechanic that is required in molecular simulation, and it can be used for basic research such as the materials science, life sciences, medication science, and chemical industry and so on. The main content of the invention are: searching the only molecular-model collection which can be used for generating the molecular field's parameter for the goal molecular; carrying quantum-mechanical calculation on the molecular-model collection to get the reference data that can be used for fitting the molecular mechanics field; fitting automatically a lot of nonlinear data; verifying automatically the fitting results; storing and managing the data and fitting results referred above with database. The invention can solve the bottle-neck problem that lacking perfect and precise force-field in the use of the molecular simulation technique abroad at present. The invention provides a new systematic method which can deduce molecular force field quickly, exactly and automatically.

The invention relates to a high simulation tissue engineering nerve repairing material NGCS and a preparing method thereof which is characterized in that the NGCS material comprises one, two or three raw materials of type I collagen, chitosan and gelatin. The NGCS material is made of the following raw materials by weight portions: 1-10 portions of type I collagen, 1 portion of chitosan and 1 portion of gelatin. The NGCS can be applied to both the basic research of the nerve injury repairing and the bridge repairing of clinical human spinal and peripheral nerve injuries or defects. The NGCS material is beneficial for the growth of nerve regenerated fiber and can be applied to the repairing of spinal and peripheral nerve injuries.

The invention provides a Period-divided and reason-divided prediction evaluation method of a natural fracture in a gas reservoir. The specific implementation scheme comprises a step of obtaining a fracture filling sample, a step of carrying out period-divided matched analysis on the sample, a step of carrying out a rock sound emission experiment to simulate and recovery the memory period of a rock fracture, a step of determining a cause type, a step of determining the formation period and reason type of a natural fracture and dividing the natural fracture system in the gas reservoir into a plurality of natural fracture sub systems with single periods and single reasons, a step of carrying out comprehensive superposition on the prediction result of each natural fracture sub system to form a prediction result, and a step of carrying out comparison analysis on the obtained prediction result and fracture development. By employing the above technical scheme, the multiple-period and multiple-reason complex natural fracture network system is divided into multiple natural fracture sub systems with single periods and single reasons to be a basic research object through period separation and reason type determination, and the precision of gas reservoir natural fracture prediction is improved.

Provided is a method for calculating dynamic characteristics of a contactor based on a piecewise linear kinetic equation, and belongs to the technical field of basic research design of general characteristics of contactors. The method comprises the steps that according to structural features of the contactor, segmentation is conducted on a contactor action process, plane collision and a continuouscollision force model with energy loss considered are used for being equivalent to a collision process, by means of the multi-body system kinematics based on a restrain method and a dynamic method, studying of the collision bounce situation of a contactor mechanical system under the action of external force is completed, and finally, dynamic characteristic calculation of the contactor with collision bounce considered is completed in combination of a coupling calculation method. The method has the advantages that the time-dependent situation of electromagnetic parameters such as the coil current and electromagnetic force in a working process of the contactor can be obtained, the electromagnetic dynamic characteristics of the contactor can be reflected more accurately, and errors are controlled within 5%; the bounce situation of an armature and a moving contact of the contactor can be reflected more really, and errors are controlled within 5%.

The invention discloses a simulation evaluation method for a configuration scheme of a safe automatic device of a transmitting-end power grid high-frequency cutting machine. The method comprises the following steps of: establishing a transmitting-end power grid frequency simulation data model comprising important components related with frequency control of a machine set side and a power grid side; setting basic research operating points of the electric power outgoing level of a transmitting-end electric network; supposing that the safe automatic device of a direct current latch-up linkage cutting machine is correctly operated, calculating two types of outgoing channel fault forms of the transmitting-end power grid for each basic research operating point one by one to obtain a simulation result of the transmitting-end electric network; supposing that the safe automatic device of the direct current latch-up linkage cutting machine fails to operate, calculating the two types of outgoing channel fault forms of the transmitting-end power grid for each basic research operating point one by one to obtain a simulation result of the transmitting-end electric network; and according to the simulation results obtained in the steps C and D, evaluating whether the effect of the configuration scheme of the safe automatic device of the high-frequency cutting machine is good. The method has the advantages of fast and accurate evaluation, comprehensive factor consideration and the like, is easy and convenient to operate, and is high in practical value.

The invention discloses a self-adaptive robust video watermarking method based on AVS (Audio Video Standard), which mainly solves the problems of high complexity, non-blindness, great gap between the current method and the AVS, and the like in the current method. When watermarks are embedded, a discrete cosine transformation low-frequency coefficient visual masking value corresponding to each subblock of video frame I is firstly calculated according to a Watson model; the energy of each subblock is sequenced in a descending order; and a large-energy subblock is selected as a watermark embedding position to be in secret key storage. Watermark embedding strength is controlled through the low-frequency coefficient visual masking value in a self-adaptive manner so as to embed the watermarks. When the watermarks are extracted, the embedded watermarking subblocks are found according to the secret key at first; and then the watermarks are extracted through comparing the relation between the subblock discrete cosine transformation low-frequency coefficient and a threshold value. In the invention, the watermark embedding and extracting arithmetic is simple; the embedding position and strength are self-adaptive; with better transparency, robustness and real-time property, the method belongs to original basic research work and meets the copyright protection needs of mass video products after the large-scale popularization and commercial application of the AVS; and the method has broad market application prospect.

The invention provides a rare earth doped hydroxyfluorapatite monocrystal nanometer wire and a preparation method thereof. The preparation method comprises the following steps: under an alkaline condition, enabling calcium nitrate, phosphate, sodium fluoride and rare earth nitrate to react with one another, thereby acquiring a hydroxyfluorapatite monocrystal nanometer wire, wherein the rare earthnitrate is at least one of lanthanum nitrate, cerous nitrate, praseodymium nitrate, neodymium nitrate, promethium nitrate, samarium nitrate, europium nitrate, gadolinium nitrate, terbium nitrate, dysprosium nitrate, holmium nitrate, erbium nitrate, thulium nitrate, ytterbium nitrate and lutecium nitrate. A monocrystal nanometer wire prepared by using the method can be further processed into a repairing material for biologic sclerous tissues, such as artificial bone, bone cement, and the like, and can be used for the basic research and clinical practice in the fields related to biological materials, organizational engineering, and the like.