118 results about "Basic research" patented technology

The invention discloses a flood process runoff monitoring method coupled with satellite remote sensing and hydrodynamics. The flood process runoff monitoring method comprises the following steps that unmanned aerial vehicle complete river topography measurement is carried out; river upstream and downstream section hydraulic parameters are obtained; constructing a digital river channel section; batch extraction of satellite remote sensing water surface width; flow calculation parameters are obtained based on the digital river section and the water surface width data; performing remote sensing inversion on the roughness of the complete riverway; and section flow monitoring and historical data inversion. A section of complete river channel is determined as a basic research unit, flow calculation precision is remarkably improved through multi-section synchronous monitoring in combination with river channel slope and roughness change dynamically captured in real time, the flowing process of water flow in the complete river channel is finely described, and the flow calculation precision is improved. The method provides long-sequence and high-reliability flow data for parameter calibration and result verification of a hydrological model, is particularly suitable for describing the flow condition when flood comes, and enables the flow condition to be closer to reality.

The invention discloses an oyster carbon sink value evaluation method, model and equipment based on carbon sequestration amount, and a medium. The method comprises the following steps: collecting oyster breeding data in an oyster breeding process; the method comprises the following steps: respectively calculating an oyster body carbon sequestration amount, an oyster attached organism carbon sequestration amount and an oyster biological sediment carbon sequestration amount according to oyster breeding data, and summarizing the oyster body carbon sequestration amount, the oyster attached organism carbon sequestration amount and the oyster biological sediment carbon sequestration amount into a total oyster breeding carbon sink amount; and calculating the carbon sink value of the oysters according to the total carbon sink amount of the oyster culture. According to the method, a traditional culture shellfish carbon sequestration basic metering method is improved, carbon sequestration amount calculation is carried out on oyster bodies and biological sediments of attached organisms respectively, the more accurate oyster culture carbon sequestration total amount can be obtained, and the carbon sequestration income of oysters is calculated based on the carbon sequestration total amount. The method is beneficial to enriching the basic research in the field of shellfish culture carbon sink accounting, and enables the ecological service value of the shellfish culture industry to be compensated through carbon sink transaction.

The invention provides an immunofluorescence detection method and system for dynamically detecting mouse uterus structure development based on multiple time points, and immunofluorescence staining comprises the steps of sheet unfolding, dewaxing, repairing, cell membrane punching, sealing, primary antibody treatment and secondary antibody treatment. The system comprises a sample pretreatment control module, a multi-channel confocal microscope imaging module and a data analysis module integrating fluorescence intensity calculation, Pearson co-localization analysis and a Western blot data integration algorithm. Through a multi-time-point and multi-index immunofluorescence detection method, dynamic and systematic data support is provided for mouse uterus development mechanism research, and an application basis is provided for fundamental research of reproductive biology and construction of related disease models.

The invention discloses a method for establishing a hemophagocytic syndrome in-vitro cell disease model, and belongs to the technical field of biology. Based on human PBMC construction, the reaction of a human immune system can be truly reflected, experimental result deviation caused by species difference in an animal model is avoided, and a more accurate model basis is provided for research of human diseases. In addition to T cell over-activation, the HLH model of the present application has a clear key feature of NK cell reduction, which is HLH, accompanied by up-regulation of cytokines such as interferon-gamma and IL-10. The establishment process of the model is rapid and efficient, the experimental period is remarkably shortened, a large number of samples or drugs can be rapidly screened, the research efficiency is improved, and the conversion process from basic research to clinical application is accelerated. The method can be used for researching various mechanisms related to the HLH, is beneficial to more comprehensive understanding of pathogenesis of the HLH, and provides a theoretical basis for developing preliminary screening of targeted drugs.

According to the application of the HTATSF1 gene in regulation and control of cattle muscle growth and development and breeding, the influence of the HTATSF1 gene on muscle growth and development and the correlation between HTATSF1-CNV and cattle growth traits are verified, a reliable molecular marker is provided for cattle seed selection and breeding, and help is provided for cattle variety improvement. A foundation is laid for association between copy number variation and growth traits of the cattle HTATSF1 gene, cattle molecular marker-assisted selection breeding work can be accelerated, the method is simple, rapid and convenient to popularize and apply, and a complete solution from fundamental research to industrial application is provided for improvement of cattle germplasm resources.

The invention belongs to the field of basic research of sandstone type uranium ore associated resource evaluation, and particularly relates to a method for judging the existence form of associated gallium in a sandstone type uranium deposit, which comprises the following steps of: measuring the contents of major elements and trace elements of a plurality of uranium ore samples, and judging whether gallium meets the lowest grade requirement of associated resources of the uranium deposit; for uranium ore samples exceeding the lowest grade requirement of gallium associated resources, correlation analysis of gallium elements, main elements and trace elements is carried out, and possible key mineral types closely related to gallium are preliminarily speculated; carrying out slice analysis, and circling an approximate range of speculated possible key mineral types; carrying out scanning electron microscope and energy spectrum analysis combined observation, and determining the existence form of gallium in the uranium deposit; the position exceeding the associated grade gallium is determined through laser in-situ analysis; the gallium-related mineral type is directly determined through electron probe analysis; and comprehensively judging the existence form of gallium in the uranium deposit. According to the method, the existence form of associated gallium in the sandstone type uranium deposit can be accurately and quickly judged.

This application discloses a method for offline analysis of powder ionization conditions, comprising the following steps: placing a silicon wafer on a target plate of a plasma device; generating a plasma beam under preset powder ionization conditions using the plasma device; injecting powder into the plasma device so that the plasma beam bombards the powder until powder injection is complete; detecting whether a first condition and a second condition are met, wherein the first condition includes at least 70% of the powder injected into the plasma device ablation and brightening upon bombardment by the plasma beam and / or forming a coating on the silicon wafer; the second condition includes the coating containing chemical bonds formed by elements of the powder and / or detecting spectral lines generated by ionization of the powder upon bombardment by the plasma beam. If the first and second conditions are met, the powder is determined to be effectively ionized. Otherwise, the powder is determined to be ineffectively ionized. The method of this application can obtain basic research data on the boronization of boron powder.

The invention discloses an antibody for specifically recognizing 1147th acetylation of ribosome biosynthetic factor 1 protein as well as a preparation method and application of the antibody. According to the protein sequence and the modification type of the ribosome biosynthetic factor 1, two modified antigen polypeptides A and B are designed, and through detection, the antibody obtained by immunizing animals with the two polypeptides has high specificity and affinity, can accurately recognize the acetylation modification form of the 1147th site of BMS1, and avoids recognition of an unmodified form or other modification sites. The antibody is suitable for various immunological detection methods, is high in repeatability and low in background, has mass production potential, can realize industrialization, not only can be used for evaluating the cis-platinum drug resistance condition in esophageal cancer treatment, but also can be used for various scenes such as basic research, disease mechanism analysis, biomarker development and drug target screening, and is wide in application range.

Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand. This method uniquely capitalizes on the redundant information stored in double-stranded DNA, thus overcoming technical limitations of prior methods utilizing data from only one of the two strands.

The invention discloses application of baclofen in preparation of a medicine for treating glioma, and belongs to the technical field of biological medicine. Through systematic in-vitro and in-vivo experiments, it is proved for the first time that baclofen (especially R-configuration) has a remarkable anti-tumor effect on glioma including temozolomide (TMZ) drug resistance. The invention not only provides a brand-new clinical strategy for treating TMZ drug-resistant glioma, but also shows important value in the field of scientific research: baclofen can be used as a valuable tool compound and is used for exploring a new function of a GABAB receptor signal channel in glioma progress and drug resistance; meanwhile, a key tool is provided for constructing a stable TMZ drug-resistant model, the high-throughput drug screening and evaluation efficiency can be remarkably improved, and the TMZ drug-resistant monoclonal antibody can serve as a positive control drug to accelerate the conversion process from basic research of glioma to clinical application and has wide scientific research application prospects and conversion potential.

The invention discloses a heteronuclear cerium-germanium complex and a preparation method and application thereof, and belongs to the technical field of organic synthesis.The heteronuclear cerium-germanium complex is a cerium-germanium complex A, a cerium-germanium complex B or a cerium-germanium complex C. Cerium and germanium centers of the complexes are in a specific space adjacent state; the cerium-germanium complex A, the cerium-germanium complex B and the cerium-germanium complex C have unique structures, so that the cerium-germanium complex A, the cerium-germanium complex B and the cerium-germanium complex C become an ideal fundamental research carrier. The method can be used for in-depth exploration of electron interaction or spin coupling possibly existing between cerium and germanium through a ligand skeleton; the two independent metal centers have synergistic reaction activity in a limited space; such structures have potential properties in molecular magnetic or luminescent materials. Therefore, the invention not only enriches the structure types of the rare earth-main group element complex, but also provides a new platform with accurate structure and adjustable performance for the basic research of multi-center metal organic chemistry, and has definite scientific significance and potential application exploration value.

The invention provides a quality detection method and application of a liver-warming decoction, and belongs to the technical field of medicine detection.The quality detection method comprises the steps that the liver-warming decoction is used for preparing a test solution, acetonitrile (A)-0.08 wt%-0.12 wt% formic acid aqueous solution (B) serves as a mobile phase, gradient elution is adopted, and the quality of the liver-warming decoction is detected through ultra-high performance liquid chromatography-tandem mass spectrometry; the obtained detection result can be used for quality evaluation or control of the whole process of research / development / production / clinical application of the liver-warming decoction. According to the present invention, the UPLC-Q-TOF-MS method is adopted to perform qualitative analysis on the substance-based chemical components of the liver-warming decoction, such that the scientific basis is provided for the substance-based material basic research of the liver-warming decoction, and the main chemical components are clarified;

The invention discloses a fluorescent probe for detecting G-quadruplex, and belongs to the technical field of biomedical detection. The fluorescent probe comprises a fusion protein, the fusion protein comprises a peptide fragment A, the peptide fragment A can be specifically combined with a G-quadruplex, and the amino acid sequence of the peptide fragment A is as shown in SEQ ID NO.1; and the cyclic rearrangement fluorescent protein is operably connected with the peptide fragment A. The fluorescent probe not only can specifically recognize in an in-vitro sample and correspond to various G4 conformations, but also can realize high-resolution imaging in living cells, promotes basic research of G4 functions, and opens up a new way for transforming medicine and clinical application.

The present invention addresses the problem of providing a method for detecting a bacterium-derived molecule contained in blood, which can be a diagnostic marker or a subject for basic research. The problem is solved by analyzing a sample prepared from a blood specimen and detecting a bacterium-derived low-molecular-weight RNA.

The invention discloses a flow field reconstruction and PIV (particle image velocimetry) measurement method based on a physical information neural network, which belongs to the technical field of fluid mechanics, deep learning, computer vision and PIV crossing and comprises the steps of low-cost experimental system construction, experimental data processing and period identification, simulation synchronization and data set construction and physical information neural network flow field reconstruction. According to the invention, the problems of high cost and non-real time of the traditional PIV technology are solved, a novel low-cost solution is provided for flow field measurement, and the popularization and application of the PIV technology in basic scientific research and teaching are promoted.

The invention discloses a gene editing system integrated with CRI SPR and an off-target risk detection method. The system comprises an sgRNA sequence module for targeting a target gene, a Cas nuclease module, a delivery carrier module and an off-target risk detection unit, the editing specificity and stability can be improved by chemically modifying sgRNA and modifying Cas nuclease, and the sgRNA is delivered to a target cell through an efficient carrier. The detection method comprises the steps of editing operation, genome DNA collection, off-target site recognition and risk level determination, and precise control is achieved through quantitative indexes such as specificity scoring, delivery efficiency and detection sensitivity (for example, S is larger than or equal to 0.95, E is larger than or equal to 80%, and L is larger than or equal to 90%). The system integrates editing and detection functions, can quantitatively evaluate the off-target risk, improves the safety and repeatability of gene editing, and is suitable for fundamental research and clinical transformation scenes.

The invention relates to the technical field of calcareous sand property research, and discloses a calcareous sand impact crushing test device which comprises a sample barrel, a supporting frame, a guide rod, a pressure applying hammer and a pressure bearing plate, the sample barrel is used for containing calcareous sand, the supporting frame is fixedly connected with the sample barrel, the guide rod is vertically arranged in the sample barrel, and the pressure applying hammer is fixedly connected with the supporting frame. The pressure applying hammer and the pressure bearing plate are arranged on the guide rod in a sleeving mode and can slide along the guide rod. Compared with the prior art, the device is simple and reliable in structure and easy to operate, the defects that an existing simple device is poor in impact precision and low in repeatability are overcome, the structure of the device can be rapidly adjusted through assembly and disassembly of a small number of components, then different tests are completed, and the device is particularly suitable for teaching, basic research and field application. The invention further provides a calcareous sand impact crushing test method, initial gradation of calcareous sand and impact parameters such as impact times and impact energy are coupled to form a model, and popularization research and teaching practice of calcareous sand impact crushing characteristics are facilitated.

By utilizing eutectic gallium–indium nanoparticles for wastewater treatment of contaminated water, holes (h+) generated through photocatalytic reactions in which the nanoparticles absorb light of specific wavelengths such as ultraviolet or visible light cause oxidation reactions that cleave bonds between carbon (C) and hydrogen (H), while electrons (e-) generated by the photocatalytic reactions cause reduction reactions that cleave bonds between carbon (C) and fluorine (F). As a result, the invention provides the advantage of decomposing and defluorinating perfluorinated compounds. Details of the project that supported this invention are as follows. [Project Identification Number] 1711189578 [Project Number] 2022R1A2C1007071 [Department Name] Ministry of Science and ICT [Project Management (Professional) Institution Name] Korea Research Foundation [Research Project Name] Personal Basic Research [Research Project Name] Dynamic Analysis on Binding and Clustering of Plasmonic Nanoparticles Through Multi-Bond Interaction [Contribution Rate] 1 / 2 [Project Performing Institution Name] Korean Foreign Language University [Research Period] 01 March 2023 to 29 February 2024. Details of another project that supported this invention are as follows. [Project Identification Number] 1485019432 [Project Number] ARQ201901019005 [Ministry Name] Ministry of Environment [Project Management (Professional) Institution Name ] Korea Environmental Industry Technology Institute [Research Project Name] Development of New Technology for Water and Sewage Treatment [Research Project Name] Development of technology for evaluating and removing trace and new pollutants in an oxidation process in a water treatment process [Contribution rate] 1 / 2 [Project Performing Institution Name] Seoul National University [Research Period] 01 March 2023 – 31 December 2023

The invention discloses a p16-OSKM transgenic mouse model and a reprogramming senescence cell construction method and application, and belongs to the technical field of cell biology and regenerative medicine. According to the method, a p16-OSKM hybrid mouse capable of specifically inducing an OSKM factor in a p16 high expression cell is obtained on the basis of hybridization of a p16-Ink4a-CreERT2 transgenic mouse, a Rosa26-CAG-LSL-rtTA3-IRES-mKate2 transgenic mouse and a Col1a1-TRE-OSKM-IRES-mCherry transgenic mouse. The selective activation of the OSKM factor in senescent cells with high expression of p16 can be realized by adding doxycycline into primary embryo fibroblasts separated from the mouse under an in-vitro culture condition, so that the senescent cells enter a cell cycle again and recover division and multiplication capacities. The technology provides an accurate model platform for basic research, and can be used for aging mechanism exploration, cell fate regulation and control, chronic disease treatment, development and screening of anti-aging drugs and the like.

The invention belongs to the technical field of biological medicines, and discloses application of a CCT6A inhibitor in preparation of a medicine for treating colorectal cancer. CCT6A is determined to be a key cancer promoting gene of colorectal cancer for the first time, the CCT6A is remarkably and highly expressed in colorectal cancer tissues and cell lines, and high expression indicates poor prognosis of patients, so that a brand-new specific target is provided for targeted therapy of colorectal cancer. The invention discloses the cancer promoting effect of the compound in colorectal cancer and the association with 5FU drug resistance for the first time, enriches the development of colorectal cancer and the molecular mechanism research of chemotherapy drug resistance, and provides a new theoretical basis and research direction for the fundamental research in the field. Experiments prove that the inhibitor can significantly reduce the mRNA level of CCT6A in colorectal cancer cells so as to strongly inhibit tumor cell proliferation and increase the sensitivity of the colorectal cancer cells to 5-FU, and a novel therapeutic drug with high specificity and high curative effect is provided for treatment of colorectal cancer.

The invention discloses a method for detecting KCNJ8 gene c.263Cgt; according to the technical scheme provided by the invention, the prevention and treatment efficiency of the hereditary arrhythmia is remarkably improved on the clinical diagnosis and treatment level. KCNJ8-A88G mutation detection is completed within 45 minutes through the ARMS-PCR technology, the mutation allele frequency as low as 0.1% can be accurately recognized through the sensitivity of 99.8%, and the diagnosis period is greatly shortened compared with traditional sequencing. On the treatment level, the KATP channel inhibitor is screened, and arrhythmia after myocardial infarction can be effectively prevented. According to the established'genotype-phenotype-treatment response 'correlation model, not only is the prevention and treatment level of diseases such as the J-wave syndrome improved, but also a conversion benchmark from laboratory basic research to clinical application is established.

The invention belongs to the technical field of molecular biology. More specifically, the invention relates to a specific sequence of a humulus lupulus Y chromosome and application of the specific sequence in sex determination. The invention provides a humulus lupulus Y chromosome specific sequence and a matched primer pair thereof. The humulus lupulus Y chromosome specific sequence and the matched primer pair can be used for carrying out accurate, efficient and stable molecular identification on the sex of humulus lupulus in a seedling stage. The primer pair Y1F / Y1R designed by aiming at the sequence shows highly consistent amplification effects in a plurality of cultivated varieties and wild materials from different ecological regions, all male samples can be stably amplified to form clear bands, and female samples do not have any non-specific amplification, so that the judgment accuracy rate reaches 100%. In conclusion, the invention provides a simple, convenient, rapid and stable technical means for sex determination of humulus lupulus, and has important application value in the aspects of breeding population construction, cross parent screening, fundamental research and the like.

The invention belongs to the technical field of aquatic pathogenic microorganism products, and particularly relates to an in-vitro culture method of enterospora hepatopenaei. The method comprises the following steps: obtaining purified spores of enterospora hepatopenaei; the method comprises the following steps: inoculating purified spores of enterospora hepatopenaei into a host cell being cultured for infection to obtain a host cell sample infected with enterospora hepatopenaei; and detecting the proliferation and maturation of the purified spores of the enterospora hepatopenaei in the host cell sample infected with the enterospora hepatopenaei by using a fluorescent staining method. The method provided by the invention provides a key technical platform for basic research, pathogenic mechanism analysis, drug screening and vaccine development of the shrimp enterospora hepatopenaei, has the advantages of convenience in acquisition of experimental materials, simplicity and convenience in operation, high repeatability and the like, and is suitable for in-vitro culture and proliferation of the pathogen in a laboratory.

The application belongs to a basic research subject in the field of computer vision, and proposes a monocular depth prediction method based on multi-path feature extraction and multi-scale feature fusion, and constructs a new encoder-decoder framework. In the encoder part, the application combines the respective advantages of convolutional neural network and Transformer, adopts a multi-path structure to reason in local and global regions at the same time, realizes fine and coarse multi-path feature extraction, and obtains diversified multi-scale feature representation. In particular, the spatial pyramid pooling module (PSP) uses multi-scale adaptive pooling operation to extract semantic information at different scales, thereby initializing the decoder features. In the decoder part, the application introduces a carefully designed encoder and decoder feature interaction module (EDFI), which fully and adaptively enhances and fuses global context semantic information and fine local edge information through a skip connection and a double-path cross self-attention mechanism. The application makes full use of the powerful spatial induction of the convolutional neural network and the global information reasoning realized by the Vision Transformer model, and at the same time, combined with a flexible lightweight decoder, the application can complete depth prediction with finer granularity and higher accuracy.

The application relates to a rotating Doppler signal optimization method based on non-complete vortex light correction. Non-complete vortex beams are very common in actual rotating Doppler speed measurement, can be used for obtaining object parameters or carrying out rotating Doppler basic research, but the mode purity of the non-complete vortex beams is reduced due to diffraction in transmission, and the experimental results are affected. Based on the diffraction characteristics of vortex beams, a rotating Doppler signal optimization method based on non-complete vortex light correction is provided. First, a superposition state vortex light distribution carrying positive and negative topological charges is subjected to phase offset angles with equal size and opposite direction, and a corrected vortex beam is obtained. After transmitting for a certain distance, the deflection of the light beam can compensate the influence of diffraction, improve the mode purity, and improve the signal-to-noise ratio of the echo signal. The method is flexible and simple to operate, can effectively overcome the influence of diffraction, improve the speed measurement accuracy and speed detection distance, and has great application value in remote sensing detection.

The invention discloses an antibody and a kit for targeting human desmogranuloprotein 2. The antibody comprises a light chain variable region and a heavy chain variable region, the heavy chain variable region comprises CDR-H1, CDR-H2 and CDR-H3, and the light chain variable region comprises CDR-L1, CDR-L2 and CDR-L3. The antibody has high affinity with deodontoprotein-2, and a kit developed by using the antibody can realize high-sensitivity quantitative detection of DSC2, thereby not only being beneficial to basic research, but also being capable of providing valuable reference information for clinical diagnosis.

The invention discloses intelligent dyeing and finishing equipment and a control system thereof, relates to the technical field of textile dyeing and finishing, and aims to solve the technical problems that in the prior art, all-around, systematic and accurate control over the production process is lacked, and intelligence and precision of the dyeing and finishing production process are difficult to achieve. And dyeing and finishing hardware equipment. The large dyeing and finishing model is a mathematical model established on the basis of physical and chemical principles obtained after fundamental research is conducted on the dyeing process, and standard reaction conditions are obtained through the mathematical model. According to the method, the large dyeing and finishing model based on physical and chemical principles is constructed, an accurate dye formula and a process rule base are generated in combination with a complex algorithm, a control system can generate reasonable parameters according to scenes, and efficient and stable production is ensured. By ensuring high-speed and reliable transmission of data, intelligent and precise dyeing and finishing production is realized, and the production efficiency and the product quality are improved.

The invention discloses a temperature field analysis method for particle type heating cigarette smoke flow heat transfer characteristics, and relates to the field of novel tobacco product basic research, the method can accurately simulate smoke flow field and temperature field distribution, and the method comprises the following steps: S1, establishing a particle type heating cigarette smoke flow scene geometric model; s2, after grid division is conducted on the model, pressure drop and heat transfer equations of different functional sections of the cigarette are built through a finite element analysis method, and a smoking time period equation and a heating temperature equation are built through a UDF function; s3, setting physical property parameters of the porous medium and related boundary conditions of heating temperature and suction pressure; s4, simulation calculation is carried out, the smoke velocity and pressure simulation result of the axial center line of the cigarette is obtained, and after the effectiveness of the model is verified through linear fitting, the temperature field and flow field distribution of the cigarette is obtained through the model. The invention provides a reliable analysis means for the related research of the granular heating cigarette.

The application discloses a thin reservoir prediction method based on frequency division phase control, relates to the field of seismic reservoir prediction, and is based on geophysical methods as a basic research means, and aims at thin reservoirs to establish variable gamma prestack deterministic inversion based on big data waveform driven low-frequency models, carry out phase control prestack geostatistics inversion combination, accurately depict internal details of thin sand layers, solve the problem of fine depiction and identification of thin layer reservoirs, and combine amplitude attributes, oil and gas detection and other methods to comprehensively verify each other, solve the geological problems of yield increase and speed increase, reduce exploration risks, and have universal applicability and broad application prospect.

The invention relates to application of a myocardial injury marker RSPO1 in preparation of a product for identifying cardiovascular diseases. The invention evaluates the expression profiles of all LGR4 ligands under cardiovascular diseases at present, provides the application of RSPO1 as a potential drug target, and further provides the application of the myocardial injury marker RSPO1 in preparation of products for identifying cardiovascular diseases based on the application. The invention also provides application of the reagent for detecting the RSPO1 protein level in preparation of products for diagnosing cardiovascular diseases. According to the invention, the problem of insufficient adaptability and specificity of the existing marker is solved, RSPO1 is developed into a diagnostic marker, a DY4645-05 ELISA kit is adopted for detection and adaptation of multiple samples such as serum, multiple cardiovascular diseases can be diagnosed, cross-species application is realized, a basic research and clinical diagnosis detection system is unified, and powerful support is provided for diagnosis and treatment of cardiovascular diseases.