186 results about "Basic research" patented technology

The invention provides a photovoltaic active distribution network voltage out-of-limit problem tracing method comprising the following steps: receiving research data and carrying out multi-scale feature analysis to obtain a standardized multi-dimensional feature matrix and a time-space incidence matrix; constructing an initial causal graph based on basic research data and power grid physical constraints, and constructing a multi-layer causal network by calculating causal intensity; extracting a dynamic feature sequence, analyzing propagation features, and generating a propagation path set and a path weight matrix; performing tensor completion and state estimation on the research data to obtain a state estimation matrix and estimation uncertainty; and outputting an out-of-limit source position, an out-of-limit risk score and early warning information based on pattern matching analysis and risk assessment. According to the invention, through combination of multi-dimensional feature analysis and a multi-layer causal network, accurate positioning and risk early warning of a voltage out-of-limit source are realized, and traceability accuracy and analysis efficiency are improved.

The invention discloses a silver carp 20K liquid phase chip and application thereof, the liquid phase chip comprises a probe combination of 20,909 SNP molecular markers covering a whole genome, the physical positions of the 20,909 SNP molecular markers are determined based on sequence alignment of the silver carp genome (GCA041475455.1), and the site information of the 20,909 SNP molecular markers is shown in the specification table 1. The invention provides a silver carp first type whole genome SNP liquid phase breeding chip, which effectively reduces the application cost of silver carp in genetic diversity analysis, QTL positioning, GWAS analysis and the like in scientific research, solves the problem that no applicable product exists in silver carp molecular auxiliary breeding and whole genome selective breeding, accelerates the progress of silver carp basic research and breeding, and has a wide application prospect. The method is of great significance in improving the utilization efficiency of silver carp germplasm resources, accelerating the breeding process of excellent characters and improving the economic value and environmental adaptability of bred varieties.

The invention relates to the technical field of biological detection, in particular to a miniaturized portable fluorescence microplate reader for instant fluorescence detection, and aims to provide a fluorescence detection scheme which is portable, high in sensitivity and quick in response. The detector integrates an MCU microcontroller, a voltage conversion module, a light source driving module, a motor driving module, a photoelectric detection module, an AD sampling module, an automatic temperature control module based on a PID algorithm, a signal processing system and a data processing system. High-sensitivity and high-resolution detection of a fluorescent sample can be realized, the concentration of the sample can be detected in real time, and the change process of the concentration of the sample can be presented through a kinetic curve. Through the optimization of the structure, optics and electronic circuits, the instrument is more miniaturized and has portability. Due to the advantages of portability, high sensitivity, quick response and the like, the kit has wide application prospects in multiple fields of basic research of nucleic acid, gene expression, sequence analysis, transgenic detection and the like.

The invention discloses a flood process runoff monitoring method coupled with satellite remote sensing and hydrodynamics. The flood process runoff monitoring method comprises the following steps that unmanned aerial vehicle complete river topography measurement is carried out; river upstream and downstream section hydraulic parameters are obtained; constructing a digital river channel section; batch extraction of satellite remote sensing water surface width; flow calculation parameters are obtained based on the digital river section and the water surface width data; performing remote sensing inversion on the roughness of the complete riverway; and section flow monitoring and historical data inversion. A section of complete river channel is determined as a basic research unit, flow calculation precision is remarkably improved through multi-section synchronous monitoring in combination with river channel slope and roughness change dynamically captured in real time, the flowing process of water flow in the complete river channel is finely described, and the flow calculation precision is improved. The method provides long-sequence and high-reliability flow data for parameter calibration and result verification of a hydrological model, is particularly suitable for describing the flow condition when flood comes, and enables the flow condition to be closer to reality.

The invention discloses an application of a marker combination in prediction or diagnosis of gout, the marker combination comprises an immune cell subset and clinical data, and the immune cell subset comprises virus-specific cytotoxic NK cells and inhibitory Vdelta2 cells. The marker combination comprises immune cell subgroups and clinical data, different immune cell subgroups are matched with different clinical data when a gout attack stage and a gout remission stage are distinguished, or when the gout remission stage and a healthy subject are distinguished, and high-sensitivity and high-specificity diagnosis results are achieved. Therefore, the marker combination disclosed by the invention has a wide prospect in the aspect of predicting different processes of gout, and can provide more basic research data for health management of gout high-risk people.

The invention discloses an oyster carbon sink value evaluation method, model and equipment based on carbon sequestration amount, and a medium. The method comprises the following steps: collecting oyster breeding data in an oyster breeding process; the method comprises the following steps: respectively calculating an oyster body carbon sequestration amount, an oyster attached organism carbon sequestration amount and an oyster biological sediment carbon sequestration amount according to oyster breeding data, and summarizing the oyster body carbon sequestration amount, the oyster attached organism carbon sequestration amount and the oyster biological sediment carbon sequestration amount into a total oyster breeding carbon sink amount; and calculating the carbon sink value of the oysters according to the total carbon sink amount of the oyster culture. According to the method, a traditional culture shellfish carbon sequestration basic metering method is improved, carbon sequestration amount calculation is carried out on oyster bodies and biological sediments of attached organisms respectively, the more accurate oyster culture carbon sequestration total amount can be obtained, and the carbon sequestration income of oysters is calculated based on the carbon sequestration total amount. The method is beneficial to enriching the basic research in the field of shellfish culture carbon sink accounting, and enables the ecological service value of the shellfish culture industry to be compensated through carbon sink transaction.

The invention belongs to the technical field of gene editing, and discloses a fusion protein, a base editing system for realizing protein multimerization and application. The fusion protein provided by the invention is a Cas9 protein in which antigen epitopes are embedded or replaced; the antigen epitope is an antigen epitope in a protein collection system; the antigen epitope can realize multimerization of the fusion protein. The base editor system comprises the fusion protein and an editing enzyme fusion protein. The editing enzyme fusion protein comprises an antibody of the antigen epitope and editing enzyme. The base editor system disclosed by the invention has the characteristics of a base editing platform which can be modularized, multimerized, programmable and extensible, and brings more possibilities and selection capabilities to the field of gene editing. The base editor system can be applied to gene editing, including application in the fields of basic research, gene therapy, animal living body editing, agricultural breeding and the like.

The invention provides a lysosome extraction method. The lysosome extraction method comprises the following steps: (1) extracting cells; (2) tissue extraction; (3) crude separation of lysosome; and (4) separating the lysosome by magnetic beads. Through EDTA-free digestion optimization, lysis solution and buffer solution formula optimization, a filter membrane interception-magnetic bead purification combined technology, a double-antibody synergistic capture strategy and a competitive mild elution system, the four core problems of low purity, large activity damage, tedious operation and strong equipment dependence in a traditional lysosome separation technology are systematically solved. Experimental data show that the lysosome yield and functional integrity of the method are obviously superior to those of a density gradient centrifugation method, and the method is suitable for trace samples and conventional equipment and has high conversion value. In the future, the technical barrier can be further optimized through automation equipment integration and multi-dimensional function verification, and the application of the method in basic research and clinical diagnosis is promoted.

Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand. This method uniquely capitalizes on the redundant information stored in double-stranded DNA, thus overcoming technical limitations of prior methods utilizing data from only one of the two strands.

The invention provides a pharmaceutical composition for treating MSS intestinal cancer, and belongs to the technical field of biological medicine, the pharmaceutical composition is used for deeply researching a treatment scheme for effectively inhibiting the MSS intestinal cancer, carrying out basic research from protein purification, proteomics, siRNA libraries and the like, and trying to develop a brand new treatment scheme and a treatment mode using nitixinone, sorafenib and PD1 antibodies; the three medicines are combined for use, so that a remarkable synergistic effect is achieved, and the tumor is successfully changed from cold to hot. The pharmaceutical composition provided by the invention has an important clinical value, breaks through the current difficulty in treatment of MSS intestinal cancer, and opens a brand new direction of application of immunotherapy in clinical advanced metastatic colorectal cancer.

The invention relates to a science and technology development stage identification and prediction method based on a life cycle matrix. The method comprises the steps of determining a direction involved in the science and technology field; scientific and technological papers and patent literatures are retrieved, time sequence data of the publication quantity of the scientific and technological papers and the application quantity of patents are obtained, S curve fitting is carried out, and time nodes of the scientific and technological field in the germination stage, the growth stage, the mature stage and the decline stage of basic research and technology application are identified; taking the basic research stage and the technical application stage as horizontal and vertical coordinates, and constructing a life cycle matrix according to a time overlapping region division result of the basic research stage and the technical application stage; and according to the life cycle matrix and the duration of each stage, an evolution trend curve of the fundamental research and technology application stage is drawn, and field growth pattern recognition and development law prediction are carried out. According to the method, the interaction relationship between the basic research and the technical application in the scientific and technological development process is demonstrated, and guidance is provided for identifying and predicting the scientific and technological development stage and trend.

The invention provides an immunofluorescence detection method and system for dynamically detecting mouse uterus structure development based on multiple time points, and immunofluorescence staining comprises the steps of sheet unfolding, dewaxing, repairing, cell membrane punching, sealing, primary antibody treatment and secondary antibody treatment. The system comprises a sample pretreatment control module, a multi-channel confocal microscope imaging module and a data analysis module integrating fluorescence intensity calculation, Pearson co-localization analysis and a Western blot data integration algorithm. Through a multi-time-point and multi-index immunofluorescence detection method, dynamic and systematic data support is provided for mouse uterus development mechanism research, and an application basis is provided for fundamental research of reproductive biology and construction of related disease models.

The invention discloses a method for establishing a hemophagocytic syndrome in-vitro cell disease model, and belongs to the technical field of biology. Based on human PBMC construction, the reaction of a human immune system can be truly reflected, experimental result deviation caused by species difference in an animal model is avoided, and a more accurate model basis is provided for research of human diseases. In addition to T cell over-activation, the HLH model of the present application has a clear key feature of NK cell reduction, which is HLH, accompanied by up-regulation of cytokines such as interferon-gamma and IL-10. The establishment process of the model is rapid and efficient, the experimental period is remarkably shortened, a large number of samples or drugs can be rapidly screened, the research efficiency is improved, and the conversion process from basic research to clinical application is accelerated. The method can be used for researching various mechanisms related to the HLH, is beneficial to more comprehensive understanding of pathogenesis of the HLH, and provides a theoretical basis for developing preliminary screening of targeted drugs.

The invention discloses a textile dyeing and finishing large model copied based on dyeing and finishing reaction conditions and application thereof, relates to the technical field of textile dyeing and finishing, and aims to solve the technical problem that the dyeing and finishing process has great uncertainty due to the fact that a data source is lacked and reaction conditions are difficult to effectively copy by means of scientific data in the prior art. Comprising a basic research module, a standard reaction condition copying module, a dyeing and finishing digital module and a dyeing and finishing large model application module. Various reaction tank tests are carried out through the basic research module, physical and chemical reaction standard data of different cloth types on auxiliaries and dyes are obtained, an accurate data source is provided for a large dyeing and finishing model, reaction conditions with high yield and good quality can be effectively copied and customized, and a proper dyeing and finishing formula and process sheet are generated. The method is beneficial to improving the quality of dyed and finished products, guarantees the production efficiency, enables the dyeing and finishing process to be more scientific and normative, and reduces defective products caused by poor conditions.

According to the application of the HTATSF1 gene in regulation and control of cattle muscle growth and development and breeding, the influence of the HTATSF1 gene on muscle growth and development and the correlation between HTATSF1-CNV and cattle growth traits are verified, a reliable molecular marker is provided for cattle seed selection and breeding, and help is provided for cattle variety improvement. A foundation is laid for association between copy number variation and growth traits of the cattle HTATSF1 gene, cattle molecular marker-assisted selection breeding work can be accelerated, the method is simple, rapid and convenient to popularize and apply, and a complete solution from fundamental research to industrial application is provided for improvement of cattle germplasm resources.

The invention belongs to the field of basic research of sandstone type uranium ore associated resource evaluation, and particularly relates to a method for judging the existence form of associated gallium in a sandstone type uranium deposit, which comprises the following steps of: measuring the contents of major elements and trace elements of a plurality of uranium ore samples, and judging whether gallium meets the lowest grade requirement of associated resources of the uranium deposit; for uranium ore samples exceeding the lowest grade requirement of gallium associated resources, correlation analysis of gallium elements, main elements and trace elements is carried out, and possible key mineral types closely related to gallium are preliminarily speculated; carrying out slice analysis, and circling an approximate range of speculated possible key mineral types; carrying out scanning electron microscope and energy spectrum analysis combined observation, and determining the existence form of gallium in the uranium deposit; the position exceeding the associated grade gallium is determined through laser in-situ analysis; the gallium-related mineral type is directly determined through electron probe analysis; and comprehensively judging the existence form of gallium in the uranium deposit. According to the method, the existence form of associated gallium in the sandstone type uranium deposit can be accurately and quickly judged.

This application discloses a method for offline analysis of powder ionization conditions, comprising the following steps: placing a silicon wafer on a target plate of a plasma device; generating a plasma beam under preset powder ionization conditions using the plasma device; injecting powder into the plasma device so that the plasma beam bombards the powder until powder injection is complete; detecting whether a first condition and a second condition are met, wherein the first condition includes at least 70% of the powder injected into the plasma device ablation and brightening upon bombardment by the plasma beam and / or forming a coating on the silicon wafer; the second condition includes the coating containing chemical bonds formed by elements of the powder and / or detecting spectral lines generated by ionization of the powder upon bombardment by the plasma beam. If the first and second conditions are met, the powder is determined to be effectively ionized. Otherwise, the powder is determined to be ineffectively ionized. The method of this application can obtain basic research data on the boronization of boron powder.

The invention discloses an antibody for specifically recognizing 1147th acetylation of ribosome biosynthetic factor 1 protein as well as a preparation method and application of the antibody. According to the protein sequence and the modification type of the ribosome biosynthetic factor 1, two modified antigen polypeptides A and B are designed, and through detection, the antibody obtained by immunizing animals with the two polypeptides has high specificity and affinity, can accurately recognize the acetylation modification form of the 1147th site of BMS1, and avoids recognition of an unmodified form or other modification sites. The antibody is suitable for various immunological detection methods, is high in repeatability and low in background, has mass production potential, can realize industrialization, not only can be used for evaluating the cis-platinum drug resistance condition in esophageal cancer treatment, but also can be used for various scenes such as basic research, disease mechanism analysis, biomarker development and drug target screening, and is wide in application range.

Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand. This method uniquely capitalizes on the redundant information stored in double-stranded DNA, thus overcoming technical limitations of prior methods utilizing data from only one of the two strands.

The invention discloses application of baclofen in preparation of a medicine for treating glioma, and belongs to the technical field of biological medicine. Through systematic in-vitro and in-vivo experiments, it is proved for the first time that baclofen (especially R-configuration) has a remarkable anti-tumor effect on glioma including temozolomide (TMZ) drug resistance. The invention not only provides a brand-new clinical strategy for treating TMZ drug-resistant glioma, but also shows important value in the field of scientific research: baclofen can be used as a valuable tool compound and is used for exploring a new function of a GABAB receptor signal channel in glioma progress and drug resistance; meanwhile, a key tool is provided for constructing a stable TMZ drug-resistant model, the high-throughput drug screening and evaluation efficiency can be remarkably improved, and the TMZ drug-resistant monoclonal antibody can serve as a positive control drug to accelerate the conversion process from basic research of glioma to clinical application and has wide scientific research application prospects and conversion potential.

The present invention discloses a method for culturing wild Ganoderma curtisii. Using a puncher, 7-mm mycelial blocks are obtained by punching at the same radius on a petri dish covered with mycelia of wild Ganoderma curtisii strains, and then inoculated on a culture medium petri dish and cultured in the dark in a constant temperature incubator at 15-30 °C. Compared with the prior art, the optimal culture conditions for the wild Ganoderma curtisii of the present invention are: carbon source starch, nitrogen source yeast powder, pH 9.0, temperature 30 °C. Under such culture conditions, the wild Ganoderma curtisii grows fastest with the best growth vigor and prefers to grow in a slightly alkaline environment; it provides basic research data for the further development and utilization of Ganoderma curtisii resources and also provides basic data reference for the research on the biological characteristics and domestication cultivation of related species of this genus.

The invention discloses a heteronuclear cerium-germanium complex and a preparation method and application thereof, and belongs to the technical field of organic synthesis.The heteronuclear cerium-germanium complex is a cerium-germanium complex A, a cerium-germanium complex B or a cerium-germanium complex C. Cerium and germanium centers of the complexes are in a specific space adjacent state; the cerium-germanium complex A, the cerium-germanium complex B and the cerium-germanium complex C have unique structures, so that the cerium-germanium complex A, the cerium-germanium complex B and the cerium-germanium complex C become an ideal fundamental research carrier. The method can be used for in-depth exploration of electron interaction or spin coupling possibly existing between cerium and germanium through a ligand skeleton; the two independent metal centers have synergistic reaction activity in a limited space; such structures have potential properties in molecular magnetic or luminescent materials. Therefore, the invention not only enriches the structure types of the rare earth-main group element complex, but also provides a new platform with accurate structure and adjustable performance for the basic research of multi-center metal organic chemistry, and has definite scientific significance and potential application exploration value.

The invention provides a quality detection method and application of a liver-warming decoction, and belongs to the technical field of medicine detection.The quality detection method comprises the steps that the liver-warming decoction is used for preparing a test solution, acetonitrile (A)-0.08 wt%-0.12 wt% formic acid aqueous solution (B) serves as a mobile phase, gradient elution is adopted, and the quality of the liver-warming decoction is detected through ultra-high performance liquid chromatography-tandem mass spectrometry; the obtained detection result can be used for quality evaluation or control of the whole process of research / development / production / clinical application of the liver-warming decoction. According to the present invention, the UPLC-Q-TOF-MS method is adopted to perform qualitative analysis on the substance-based chemical components of the liver-warming decoction, such that the scientific basis is provided for the substance-based material basic research of the liver-warming decoction, and the main chemical components are clarified;

The invention discloses a fluorescent probe for detecting G-quadruplex, and belongs to the technical field of biomedical detection. The fluorescent probe comprises a fusion protein, the fusion protein comprises a peptide fragment A, the peptide fragment A can be specifically combined with a G-quadruplex, and the amino acid sequence of the peptide fragment A is as shown in SEQ ID NO.1; and the cyclic rearrangement fluorescent protein is operably connected with the peptide fragment A. The fluorescent probe not only can specifically recognize in an in-vitro sample and correspond to various G4 conformations, but also can realize high-resolution imaging in living cells, promotes basic research of G4 functions, and opens up a new way for transforming medicine and clinical application.

The invention discloses a construction method of a lung cancer transplantation tumor model. The construction method comprises the following steps: S1, constructing an asthma model for rodents by adopting allergens causing asthma; s2, the lung cancer transplantation tumor model is established by transplanting rodent-derived lung cancer cells in the asthma model. As a novel lung cancer transplantation tumor model, the lung cancer transplantation tumor model constructed by the invention can well simulate the discovery of clinical research in basic research, and the scientific value and the application value of the lung cancer transplantation tumor model are improved.

The invention provides a quality detection method and application of Shuidi decoction, and belongs to the technical field of medicine detection.The quality detection method comprises the steps that the Shuidi decoction is used for preparing a test solution, acetonitrile (A)-0.08-0.12 wt% phosphoric acid aqueous solution (B) serves as a mobile phase, and the quality of the Shuidi decoction is detected through ultra-high performance liquid chromatography-tandem mass spectrometry; the obtained detection result can be used for the quality evaluation or control of the whole process of research / development / production / clinical application of the Shuidi decoction. According to the present invention, the UPLC-Q-TOF-MS method is adopted to perform qualitative analysis on the substance-based chemical components of the Shuandii decoction, such that the scientific basis is provided for the substance-based substance fundamental research of the Shuandii decoction, and the main chemical components of the Shuandii decoction are clarified;

The invention belongs to the technical field of biological medicine, and particularly relates to an anti-CXCL1 nano antibody and application thereof. The nano antibody comprises a heavy chain variable region targeting CXCL1, and the heavy chain variable region is composed of FR1, CDR1, FR2, CDR2, FR3, CDR3 and FR4; the amino acid sequence of the nano antibody and any one of SEQ ID NO.1-49 have at least 70% of sequence identity. The nano antibody has the advantages of small molecular weight, small immunogenicity and better solubility and stability, is beneficial to basic research and clinical tests, has potential diagnosis and treatment prospects for tumors, autoimmune diseases and neurological diseases, and has good practical application value.

The present invention addresses the problem of providing a method for detecting a bacterium-derived molecule contained in blood, which can be a diagnostic marker or a subject for basic research. The problem is solved by analyzing a sample prepared from a blood specimen and detecting a bacterium-derived low-molecular-weight RNA.

The biomarker, the analysis method and the application can be effectively used for early diagnosis, disease course monitoring and prognosis evaluation of asthma, are high in sensitivity, rapid, convenient and accurate and reliable in result, can be used for multiple times of monitoring in a clinical control period, a chronic duration period and an acute attack period of an asthma patient, and provide a basis for clinical decision making. Meanwhile, a certain basis is provided for subsequent fundamental research and clinical research, and the method has great application and research values.

The invention discloses a chrysanthemum subterranean stem efficient genetic transformation method independent of tissue culture, which comprises the following steps: selecting subterranean stems, cutting the subterranean stems into segments, infecting the segments with agrobacterium tumefaciens, and sequentially performing dark culture and culture under illumination to obtain transgenic positive buds. The method is simple in steps, short in experimental period, high in efficiency and low in cost, a large number of transgenic buds can be obtained in a short time, the transgenic buds can be directly cut into vermiculite to survive, and exogenous genes are stably expressed; the chrysanthemum subterraneous stem transformation system independent of tissue culture is established for the first time, and effective technical support is provided for basic research and scientific research breeding of chrysanthemum.