8 results about "Palmatine" patented technology

The invention provides a method for preparing palmatine. According to the method, L-tetrahydropalmatine is used as at least part of raw materials. The method for preparing the palmatine from the L-tetrahydropalmatine has the advantages of simplicity in operation, environmental protection, low cost and the like, and is high in application value.

The invention belongs to the technical field of medicinal chemistry and green chemical industry, and particularly relates to a green circulation method of rotundine resolution mother liquor. According to the method, the acid mother liquor generated after chiral resolution of rotundine (L-tetrahydropalmatine) tartaric acid is used as a raw material, separation and purification are not needed, the acid environment of the mother liquor is directly utilized, hydrogen peroxide is used as a green oxidizing agent, and the target product is obtained. The method comprises the following steps: oxidizing a residual tetrahydropalmatine mixture (including dextroisomer, unresolved racemate and lost levoisomer) in a mother solution into palmatine salt; then, an alkaline reducing agent is directly added into the reaction system without separation, the palmatine salt is reduced into racemic tetrahydropalmatine, meanwhile, the reducing agent or a hydrolysate of the reducing agent is neutralized with residual acid in the oxidation step, and self-adjustment of the pH of the system is achieved. According to the method, a post-treatment strategy of combining a one-pot method with a solvent devitrification resistance is creatively adopted, and the product is directly and efficiently separated out from a reaction system by simply adjusting pH and diluting, so that the process is greatly simplified. The method abandons the scheme of using expensive and toxic oxidants such as iodine or N-halogenated imide in the traditional process, has the remarkable advantages of low cost, environmental friendliness, high atom economy, short process flow and extremely small wastewater generation amount, and is very suitable for industrial production.

The invention discloses a method for efficiently screening a functional enzyme and application of the functional enzyme, a demethylase is rapidly screened through matrix-assisted laser desorption / ionization mass spectrometry (MALDI-MSI) combined with non-targeted metabonomics and transcriptomics analysis, and the demethylase can be converted into dihydroberberine by taking palmatine as a substrate, so that the functional enzyme can be efficiently screened. And the enzyme mining efficiency and accuracy are obviously improved. The functional enzyme mining strategy provided by the invention solves the problem that more comprehensive information of natural metabolites and functional enzymes cannot be obtained by a traditional method, and is more beneficial to environmental protection and sustainable development.

The present application provides a kind of palmatine derivative, the structural general formula is as follows: the present application selects palmatine as raw material, through multi-step synthesis, corresponding compound with high yield can be obtained, the palmatine derivative can effectively resist antibiotic resistant helicobacter pylori strain, especially spherical mutant strain, has good bacteriostatic activity, solves the antibiotic resistance and retention recurrence problem of helicobacter pylori in clinical treatment, is suitable for industrial production and clinical application.

The invention relates to a dactylicapnos root O-methyltransferase DsOMT011 gene and application thereof, and belongs to the technical field of biology. The nucleotide sequence of the dactylicapnos root O-methyltransferase DsOMT011 gene is shown as SEQ ID NO.1, the amino acid sequence of the encoded protein is shown as SEQ ID NO.2, and the dactylicapnos root O-methyltransferase DsOMT011 gene can be used for preparing palmatine. According to the invention, a synthetic pathway and gene family evolution of the dactylicapnos alkaloid are accurately analyzed in basic research; in application and development, accurate targets are provided for directional synthesis of high-value components and innovative breeding; and finally, the dactylicapnos scandens is promoted to be upgraded from experiential medicinal use to molecular regulation and accurate utilization, which is a value which is difficult to cover in the prior art.

The invention belongs to the technical field of medicines and disease treatment, and particularly relates to a traditional Chinese medicine composition for treating atherosclerosis and a preparation method and application thereof. The composition is prepared by taking rhizoma pinellinae praeparata, rhizoma coptidis, pericarpium trichosanthis, radix angelicae sinensis, rhizoma chuanxiong, ground beeltle, radix salviae miltiorrhizae and radix ginseng as raw medicinal materials and adding sucralose and maltodextrin. Wherein berberine and palmatine in coptis chinensis are core active components, and play a role in regulating blood fat, inhibiting inflammation and blocking foam cell formation. Researches show that the particles can significantly reduce the serum total cholesterol, triglyceride and low-density lipoprotein cholesterol levels of model animals, increase the high-density lipoprotein cholesterol level, reduce the aortic plaque area and inhibit RAW264.7 cell foaming, the curative effect is equivalent to that of simvastatin, and a new choice is provided for prevention and treatment of atherosclerosis.

The invention relates to the technical field related to biological medicine, in particular to a method for inhibiting cholesterol esterification of macrophages, through targeted regulation of a PPAR-gamma / LXR-alpha signal channel by means of palmatine (PAL), foam cell formation can be effectively reduced, and accordingly atherosclerosis (AS) can be improved. Oxidized low-density lipoprotein (ox-LDL) is used for inducing foaming, then 25-100 [mu] g / ml palmatine is used for intervening for 48 hours, and experiments show that the palmatine remarkably reduces the cholesterol esterification rate (from 52.17% to 41.88%, Plt; according to the present invention, with the application of the palmatine in the preparation of the medicine, the expression of cholesterol efflux genes such as ABCA1, ABCG1 and the like is up-regulated, the release of inflammatory factors IL-6 and TNF-alpha is inhibited (reduced by more than 60%), and the molecular docking proves that the combination of the palmatine and the PPAR-V is stable (the combination energy is-8.482 kcal / mol), and is superior to the traditional medicine; the invention provides a new strategy for natural, safe and multi-target intervention of AS, and has significant clinical application potential.

The application discloses a dual-unit fluorescent sensor array for detecting oral anti-diabetic drugs and application thereof, and relates to a supramolecular fluorescent sensor array which comprises a PAL@Q[8] sensing unit and a bpep-Am@Q[8] sensing unit, wherein the PAL@Q[8] sensing unit is composed of palmatine and Q[8], and the bpep-Am@Q[8] sensing unit is composed of a derivative of 1,4-bis[2-(4-pyridyl)vinyl]benzene and Q[8]. The dual-unit fluorescent sensor array can be used for qualitative detection and quantitative detection of metformin, phenformin, buformin hydrochloride, rosiglitazone and sitagliptin in serum or urine, and the method provides a simple and reliable analysis tool for rapid screening of oral hypoglycemic drugs, clinical blood drug concentration monitoring and identification of hypoglycemia causes, so as to realize on-site rapid visual analysis of oral hypoglycemic drugs.