119 results about "Palmatine" patented technology

The invention discloses a total alkaloid extract and making method of eye drip, which comprises the following parts: opium alkaline, tetrahydropalmatine, cumic acid, alcaine palmatine, corydaline, summer alkaline and other biological alkalines. The preparing method comprises the following steps: adopting summer non as raw material; optimizing hypercritical CO2 flow to extract; collecting extract; drying; obtaining the total alkalilne extract; fitting for tablet, pill, suppository, percutaneous absorption agent, oral rapid-breaking agent, release-control agent and freeze dried.

A process for preparing the refined palmatine from general fibraurea alkaloid faetures that the principle that different kinds of alkaloid have the difference in pH value and solvent polarity is used for separation or the column photography is used for separation, and the relative refining technique is also used to make its purity be 99.0% or more.

The invention discloses a synthesis process of palmatine and its salts mainly comprising the following steps, (1) etherification, preparation of o-dimethoxybenzene, (2) acetonitrilizaiton, preparation of methylenedioxy benzene acetonitrile, (3) hydrogenization, preparation of o-dimethoxy-phenethylamine, (4) condensation, preparation of condensate hydrochlorates, (5) recondensation, preparation of palmatine, (6) preparation of corresponding salts from palmatine with related acids.

The invention discloses a new synthesizing method of 13-n-octyl berberine (1) and 13-n-octyl palmatine (2) as berberine derivant and medical acceptable salt and liposome, which is characterized by the following: inhibiting the growth of cancer cell obviously through S180 transplanted tumour model test under lower dose condition; saving cost.

The invention discloses application and a preparation of synthetic palmatine (or inorganic salts, such as hydrochloride, sulfate, phosphate, and the like, and organic salts or derivatives) prepared by a chemical synthetic method, the preparation of raw materials and the fine preparation of raw materials. The synthetic palmatine are needle-like crystals or particle crystals of light yellow to dark yellow. Firstly proved by pharmacological and toxicological substitution research, the synthetic palmatine can be used as medicines instead of palmatine extracted from plants, has better effects than the palmatine extracted from plants in the curative effect aspects of resisting inflammation, bacteria, viruses, and the like, is synthesized by the disclosed methods or the method in the invention, can be prepared into injections, and like to be applied to clinical departments of resisting bacteria, eliminating inflammation and resisting inflection.

The invention belongs to the technical field of pharmacy, and particularly discloses a stephania hainanensis total alkaloid extract extracted from stephania hainanensis tubers and a preparation method thereof. The preparation process is as follows: material (stephania hainanensis dry tubers); grinding; soaking in a moderate amount of 0.01 to 10 percent of hydrochloric acid or sulfuric acid; 1 to 4 times of ultrasonic extraction; filtration; storng-acid cation exchange resin purification; solvent recovery for drying; and stephania hainanensis total alkaloid extract. The extract mainly comprises L-tetrahydropalmatine, palmatine, fangchinoline, hanfangchin and other active ingredients, the percentage by weight of stephania hainanensis total alkaloid is greater than or equal to 70 percent, the percentage by weight of the palmatine is greater than or equal to 20 percent, and the percentage by weight of the fangchinoline is greater than or equal to 40 percent. Reports on the stephania hainanensis total alkaloid extraction process are not found in related literatures so far, the operation flow of the method is simple, the purity of the product is high, the efficacy is remarkable, and the method disclosed by the invention is applicable to industrial production and the exploitation and utilization of local stephania hainanensis resources.

The invention discloses an antibacterial finishing agent, which comprises, by mass: 2-3% of a china root greenbrier extract, 10-15% hydroxypropyl methyl cellulose, 5-10% of the palmatine, 30-35% of rosin based waterborne polyurethane, 2-3% of butyl ricinoleate sodium sulfate, 8-10% of OP-10, and the balance ethanol. The antibacterial finishing agent provided by the invention has a good bacteriostatic effect, and is green and environment friendly.

The invention relates to a protein fluorescence detection technology, in particular to the application of the THP and the derivatives in the protein fluorescence detection. The invention also provides a method to apply the THP to the protein fluorescence gel detection, and the method comprises the steps of: fixing the electrophoresis gel of the protein sample in the stationary liquid, washing by the deionized water; dyeing, washing by the deionized water; detection. The invention has the advantages of high sensitive, simple and rapid operation, good reproducibility, good dyeing background, good linear relationship, good reversibility, good compatibility, safe using and low cost, etc.

The invention relates to a thirst quenching clearing particle content detection method, the method comprises the following steps: preparation of a to-be-tested sample solution, to be more specific, taking a thirst quenching clearing preparation, adding proper amount of extraction solvent methanol or ethanol, ultrasonically dissolving, adding a solvent to adjust the solution concentration, filtering with a microfiltration membrane to obtain filtrate; preparation of a control stock solution, to be more specific, taking neomangiferin, mangiferin, timosaponin BII, palmatine and berberine controls, adding methanol for dissolving to obtain the control stock solution; determination of content, to be more specific, respectively injecting the to-be-tested sample solution and the control stock solution into UPLC-PDA-ELSD chromatograph to obtain a chromatogram, and according to the chromatogram, using the peak area method for calculation of content of the neomangiferin, mangiferin, timosaponin BII, palmatine and berberine.

The invention provides a method to detect effective ingredients of radices puerarire, radices scutellariae and coptis chinensis medicine (Gegen Qin Lian Medicine) by high efficiency liquid chromatogrophy. The method consists of the following steps: firstly, a sampling liquid to be detected and a mixing control solution are prepared, which are then respectively detected by the following methods: a chromatographic column stuffed by octadecyl silane bonded silica gel is injected by the sample size of 1 to 20ul, the column temperature is controlled between 10 to 40 DEG C, then gradient elution is carried out by a mobile phase which is composed of phase A and phase B at a flow velocity of 0.3 to 1.5ml/min according to the stipulated procedure; moreover, multi-wavelength ultraviolet is employed to detect the eluent so as to obtain the high efficiency liquid chromatograms of the detected medicines and the control solution; finally, the high efficiency liquid chromatograms of the detected medicines and the control solution are compared so as to determine the effective components of puerarin, daidzin, wogonoside, baicalin, palmatine and berberine and the contents thereof. The phase A in the method of the invention is ammonium acetate solution with the concentration of 0.01 to 0.1mol/l, and the phase B is acetonitrile.

The invention discloses effects of palmatine and a composition thereof for preventing and treating senile dementia; the palmatine and the composition thereof are extracted from fibraurea recisa pierre of fibraurea lour. of menispermaceae and same generic plants; enzymology and animal experiments prove that both the palmatine and the composition thereof have good effects of preventing and treating senile dementia; further researches show that the activity of the palmatine is better than that of the composition; and the fact shows that the palmatine is a main pharmacodynamic substance.

The invention discloses a preparation method of fibrauretin which is applied in the chemical synthesis field of broad-spectrum antibiotics. The preparation method comprises the following steps: adding berberine hydrochloride in an organic solvent, and reacting at 100-150 DEG C under the catalysis of a Lewis acid to remove methylene and obtain an intermediate 2,3,9,10-tetrahydroxyberberine; placing the intermediate 2,3,9,10-tetrahydroxyberberine in a basic solvent to react under the action of a methylating agent at 30-40 DEG C and obtain an intermediate fibrauretin base; and heating the intermediate fibrauretin base in distilled water to dissolve, and adding hydrochloric acid to form the salt and obtain fibrauretin, wherein the organic solvent is selected from one of toluene and xylene, the Lewis acid is selected from one of aluminium chloride, boron tribromide and zinc chloride, and the methylating agent is dimethyl sulfate. The chemical synthesis method is adopted to prepare fibrauretin, thus the plant resource can be saved and the ecological environment can be protected; and berberine hydrochloride is used as the raw material, the raw material is cheap and easily available, the reaction steps of the synthetic route are short and the operations are simple, thus the cost can be saved and the method is suitable for industrial production.

The invention relates to determination of alkaloid content in decumbent corydalis tuber total alkaloid extractive. The determination method comprises the following steps: (1) preparing a decumbent corydalis tuber total alkaloid extractive solution; (2) preparing a standard solution; (3) respectively sucking the extractive solution and the standard solution of an equal amount, and carrying out UFLC determination to obtain a UFLC chromatogram; and (4) analyzing chromatographic peaks of protopine, bicuculline, xiawuning alkaloid, corydaline harmine, tetrahydropalmatine and palmatine in the chromatogram, and calculating contents thereof by an external standard method. Besides, the invention also provides a method for establishing a UFLC finger print of decumbent corydalis tuber total alkaloidextractive. The determination method can quickly and accurately determine contents of six main alkaloids in decumbent corydalis tuber total alkaloid extractive, and the UFLC finger print of decumbentcorydalis tuber total alkaloid extractive can be used for the overall control on substance groups in decumbent corydalis tuber total alkaloid extractive, thereby controlling the quality of extractivefrom the macro analysis perspective.

The invention relates to a novel preparation method of palmatine.

The invention relates to a method and a device for enhancing the dissolution rate of effective components in coptis chinensis, particularly to a method and a device for enhancing the dissolution rate of effective components in coptis chinensis by electron beam irradiation. The invention can solve the problem that existing traditional Chinese medicine extraction methods cannot have the characteristics of high effective component dissolution rate, high purity, low cost and suitability for industrial production at the same time. According to the method, electron beams are employed to irradiate the coptis chinensis medicinal material. The device for realizing the method of enhancing the dissolution rate of effective components in coptis chinensis by electron beam irradiation comprises an electron accelerator scanning box, an aluminum plate, a heat conduction instrument, a beam receiving plate, an insulation material body, cooling fans and a beam integrator. By adopting the method provided by the invention, the dissolution rate of berberine in the effective components is increased by 5%-10% compared with a blank sample not subjected to electron beam irradiation, the dissolution rate of palmatine is increased by 4.5%-11% compared with the blank sample not subjected to electron beam irradiation, and electron beam irradiation on the coptis chinensis medicinal material has no significant influence on chemical components. The method and the device can be used for extraction of effective components in coptis chinensis.

The invention discloses a Chinese medicament for treating acute enteritis. The Chinese medicament for treating acute enteritis is prepared from 20 Chinese medicaments in a certain weight part ratio, namely humifuse euphorbia herb, malt, baicalin, rice sprout, puerarin, plantain seed, berberine, bitter orange, rhizome of Chinese atractylode, jateorhizine, phoenix-tail fern, coix seed, palmatine, amomum fruit, honeysuckle, weeping forsythia, dandelion, glycyrrhetic acid, gordon euryale seed and daidzin. The Chinese medicament for treating acute enteritis has the characteristics of low cost, relatively high curative ratio and the like, and is convenient to prepare, and materials are easy to obtain.

The invention relates to palmatine ophthalmic gel and a preparation method thereof. The method comprises the following steps: adding water for injection into a matrix and an antioxidant in a formula dosage, mixing, swelling or dissolving; adding a pH regulator for uniformly stirring, thereby obtaining a matrix liquid; weighing palmatine according to the formula dosage, adding the water for injection for dissolving, adding a preservative and an isoosmotic adjusting agent, uniformly mixing, adding the mixture into the matrix liquid, adding the water for injection to 1L, uniformly shaking, filtering by virtue of a micro-aperture filter membrane, sterilizing, and split charging, thereby obtaining the product. The gel comprises the following components: 0.5-30g of palmatine, 0.1-30g of a matrix, 0.1-20g of a pH regulator, 1-100g of an isoosmotic adjusting agent, 0.01-8g of a preservative and 0.02-5g of an antioxidant. Compared with palmatine liquid type eye drops, the product disclosed by the invention has certain advantages in the dosage form, the administration frequency can be reduced, loss of the medicine in the eyes is avoided, and the effect of the medicine for treating conjunctivitis and keratitis can be obviously improved.

The invention provides an anti-acetylcholinesterase active composition in Chinese mahonia stem, and a screening method and application of the anti-acetylcholinesterase active composition. The composition is prepared from columbamine, jateorhizine, palmatine and berberine. According to the anti-acetylcholinesterase active composition, the traditional Chinese medicine Chinese mahonia stem is taken as a study object, based on an affinity chromatography principle, an immobilized enzyme technology is combined, an acetylcholinesterase reactor is prepared, an active compound fishing platform is constructed, four active components for anti-acetylcholinesterase are successfully screened and identified, an effective screening method for discovery of extracting anti-acetylcholinesterase active substances from the Chinese mahonia stem, when the compounds columbamine and jateorhizine are fixed, other compounds are correspondingly added to be mixed with the columbamine and the jateorhizine, when theconcentration of the columbamine or the jateorhizine is below IC60 or IC70, the obvious synthesis or summation effect occurs, according to the research, the compound components developing the anti-AChE activity in the Chinese mahonia stem extraction are discovered and identified for the first time, the combination with synthesis or summation is obtained, and the combination can be assuredly developed to a potential anti-AChE inhibitor.

The invention discloses an extraction method and a detection method for berberine and palmatine in cortex phellodendri chinensis. The extraction method comprises the following steps: crushing dried cortex phellodendri chinensis and carrying out sieving with a 60-mesh to 80-mesh sieve; adding obtained powder in to a solvent according to a solid-liquid mass ratio of 1:10 to 1: 50; soaking the powder for 24 h at a normal temperature after mixing and sealing; placing an obtained mixture in a high pressure vessel and applying a pressure of 100 to 500 MPa at a temperature of 20 to 60 DEG C for extraction, with holdup time being 1 to 5 min; and centrifuging an extractive after pressure relief, taking a supernatant and carrying out filtration so as to obtain an extract of cortex phellodendri chinensis; wherein the solvent is selected from the group consisting of water, ethanol with a concentration of 10 to 90%, methanol with a concentration of 70% and acetone with a concentration of 70%. The supernatant is subjected to HPLC quantitative analysis so as to realize detection. According to the invention, structural changes and loss and reduction in physiological activity of an effective constituent caused by heat effects are avoided, extraction time is short and consumes a small amount of energy, and the detection method is simple, accurate and rapid and has high compactness and good repeatability.

The invention discloses a process method for alcohol extraction of palmatine. According to the process method, the palmatine extracting rate is increased, the extraction time is shortened, the production efficiency is improved, filtrate is easy to filter, the labor intensity is reduced, the use amount of acid water is reduced, and therefore, the use amount of salting out raw materials and the sewage treatment amount are reduced. According to the technical scheme, the process method comprises the following steps of: extracting common fibraurea stem powder with alcohol, combining filtrate, cooling, and filtering to remove insoluble impurities; drying the filtrate through steaming, and eluting residues after the steaming with acid liquid; filtering eluant, regulating the alkali degree, standing until the insoluble impurities are separated, filtering, adding sodium chloride into the filtrate, carrying out salting out, and filtering to obtain a crude product; and heating to dissolve the crude product into ethanol, immediately filtering to remove the insoluble impurities, after the filtrate is cooled, slowly adding concentrated hydrochloric acid, after palmatine hydrochloride is separated out, filtering, and drying, so as to obtain the product.

The invention discloses a technology for extracting palmatine from a common fibraurea stem and belongs to the technical field of palmatine preparation. The palmatine is prepared through the steps of crushing, primary decoloration, crystallization, secondary decoloration, recrystallization, extraction, separation and purification, concentration and crystallization and drying. The yield of the palmatine prepared by the method is greater than 38.25% and the purity is greater than 98.31% and higher than that of the palmatine prepared in the prior art, and the palmatine can be used as a qualitative and quantitative reference substance.

The invention discloses an HPLC measurement method for content of multi-index components in rhizoma corydalis pain relieving drop pills. A high performance liquid chromatography (HPLC) method is adopted; a mixed contrast solution is used as a contrast, and tetrahydropalmatine is used as a reference; meanwhile, 7 main components, including protopine, palmatine, dehydro-corydaline, tetrahydropalmatine, corydaline, imperatorin and isoimperatorin, in the rhizoma corydalis pain relieving drop pills are measured simultaneously. According to the method, QAMS is realized; measurement of multiple components is realized by adopting relative correction factors among the components; the quality of the multiple components of a traditional Chinese medicine is controlled, thus realizing evaluation on quality of multiple indexes of the rhizoma corydalis pain relieving drop pills. The method is easy and convenient to operate, and quick; the detection cost and time are greatly reduced; the working efficiency and the practicability of the method are improved; the quality of traditional Chinese medicinal products can be controlled more effectively, comprehensively and accurately; stability and uniformity of the product quality are guaranteed, so that the aim of safe and effective taking of the product is fulfilled.

The invention relates to an anti-bacterial inflammation-diminishing traditional Chinese medicine suppository as well as a preparation method and quality control method thereof, and belongs to the technical field of traditional Chinese medicines. The anti-bacterial inflammation-diminishing traditional Chinese medicine suppository comprises the following components in parts by weight: 48-52 parts of alumen, 8-12 parts of palmatine, 38-42 parts of red halloysite, 8-12 parts of borneol, 18-22 parts of polysorbate 80 and 900-920 parts of glycerin gelatin. The suppository provided by the invention is simple in preparation method, and the prepared product is excellent in performance, has the effects of preventing bacteria, diminishing inflammation and swelling, relieving pain, stopping bleeding, restraining and removing necrotic tissue and promoting granulation, is remarkable in effect, and can be widely used for preventing and treating anorectal and gynecological inflammatory diseases. Moreover, the curative effect of the product is guaranteed through control by virtue of the quality control method.