2607 results about "Inflammatory factors" patented technology

The invention discloses bacillus velezensis as well as fermentation product filtrate and a biological product thereof, and belongs to the technical field of microorganisms, a bacillus velezensis BTN-HB-F4 strain is separated and purified from prinsepia utilis royle in Harba snow mountain in Yunnan province, the fermentation product filtrate of the bacillus velezensis has excellent HAS inhibition capacity and remarkable DPPH and ABTS free radical scavenging capacity, and the biological product of the bacillus velezensis has the advantages that the biological product can be used for preparing biological products of the prinsepia utilis royle; a good anti-oxidation effect can be realized; secondly, under various concentrations, fermentation product filtrate can remarkably reduce the proportion of SA-beta-Gal positive cells, gene expression of matrix metalloproteinase 3 can be remarkably inhibited, and a good anti-aging effect is achieved; in addition, fermentation product filtrate can remarkably inhibit generation of zebra fish melanin and has a good whitening effect; the secretion amount of cell inflammatory factors IL-6 and TNF-alpha can be remarkably inhibited, and a good relieving effect is achieved; finally, the fermentation product filtrate can effectively inhibit the growth of pathogenic bacteria staphylococcus aureus, and has the potential of regulating the skin micro-ecology.

The invention discloses a photoacoustic spectrum-confocal Raman spectrum multi-view-field high-dimensional imaging method and a photoacoustic spectrum-confocal Raman spectrum multi-view-field high-dimensional imaging device. Nanosecond pulse laser with adjustable wavelength is generated through a continuous spectrum nanosecond pulser and a narrow-band spectrum tuning module and cooperates with a microscopic detection module, a depth imaging module, a galvanometer system and an electric displacement table to obtain spatial three-dimensional data, a photoacoustic spectrum and a Raman spectrum under multiple view fields; shallow photoacoustic spectrum-Raman spectrum tomography and deep three-dimensional photoacoustic imaging are realized; correcting a photoacoustic spectrum quantitative error by using a spectrum decoupling algorithm, and fusing the multi-view-field data to generate multi-view-field high-dimensional photoacoustic spectrum-Raman spectrum data; high-dimensional data features are extracted based on a 3D convolution instance segmentation network, and quantitative analysis and spatial distribution visualization of blood oxygen, inflammatory factors and the like are achieved. According to the method, the limitation that substances are difficult to analyze in photoacoustic imaging is overcome, the contradiction between the imaging resolution and the penetration depth is solved, and an accurate analysis means is provided for diagnosis of diseases such as knee osteoarthritis and sepsis.

The invention relates to the field of microorganisms, and discloses lactobacillus plantarum and application of the lactobacillus plantarum in preparation of a medicine for preventing and / or treating vaginitis, and the preservation number of the lactobacillus plantarum is CGMCC No.33152. The lactobacillus plantarum provided by the invention has a relatively strong bacteriostatic effect on common vaginal pathogenic bacteria such as Atortoria, staphylococcus aureus, Candida albicans, Escherichia coli and Gardneria, and also can reduce the infection degree of the pathogenic bacteria by inhibiting colonization and reproduction of the pathogenic bacteria; in addition, the proinflammatory factor level in sick vaginitis tissues is reduced, and the anti-inflammatory factor level is improved.

The invention discloses preparation and application of bionic cerium-based polyphenol nano-enzyme for treating rheumatoid arthritis, and belongs to the field of medical preparations. The method comprises the following steps: respectively complexing cerium ions and tannic acid or epicatechin gallate to prepare cerium-based polyphenol nano-enzyme, and then preparing the cerium-based polyphenol nano-enzyme wrapped by a macrophage membrane. And loading the cerium-based polyphenol nano-enzyme wrapped by the macrophage membrane into the hydrogel to prepare the hyaluronic acid-based hydrogel. The cerium-based polyphenol nano-enzyme and the hyaluronic acid-based hydrogel are characterized, it is verified that the cerium-based polyphenol nano-enzyme and the hyaluronic acid-based hydrogel are successfully synthesized, the particle size of CeTA is about 22 nm, the particle size of M-CeTA is increased to 273 nm, the particle size of CeECG is about 95 nm, and the particle size of M-CeECG is increased to 292 nm. In-vitro experiments prove that the M-CeTA and the M-CeECG do not show obvious cytotoxicity when being less than 80 mu g / mL, and have an obvious inhibition effect on expression of cell inflammatory factors on inflammatory cells.

The invention belongs to the technical field of microbial fermentation, and particularly relates to lactobacillus rhamnosus capable of maintaining bone health and improving local joint inflammation and a metagen and application of the lactobacillus rhamnosus. According to the invention, the probiotic NKU ML1-2 is subjected to fermentation culture, such that a post-generation product is prepared. Results of embodiments prove that the metagen product can enhance the oxidation resistance of cartilage and osteoblasts and promote the synthesis of anti-inflammatory factors and cartilage matrixes (type II collagen and proteoglycan), and has the potential of relieving oxidative stress and inflammatory response of local joints, relieving joint inflammation and delaying cartilage destruction. The prepared metagen product is high in safety and good in stability, and can be applied to preparation of medicines for preventing and / or treating osteoarthritis; the metagen product can also be used for promoting bone health, enhancing the antioxidant capacity of osteoblasts and / or chondrocytes, promoting osteoblast activity and / or promoting calcium absorption and fixation, and a new solution is provided for joint health management.

The invention provides a preparation method of cereal fermentation broth postbiotics and application of the cereal fermentation broth postbiotics in improvement of systemic chronic inflammation and reduction of cholesterol and low-density lipoprotein, and belongs to the technical field of fermentation engineering. The invention provides a preparation method of cereal fermentation broth metabiotics, which is characterized in that mixed cereals are used as raw materials, anaerobic fermentation is carried out by using compound enzymes and various bacteria, and polysaccharides and proteins in the cereal raw materials can be quickly converted into functional small molecular substances, such as polypeptides, total phenols and total flavonoids. The cereal fermentation broth metagen can inhibit secretion of a macrophage proinflammatory medium, inhibit generation of inflammatory factors of liver, heart and intestinal tissues, and improve activation of adipose tissue macrophages related to body inflammation. After the cereal fermentation liquor is subjected to post-biogen treatment, glucose and lipid metabolism disorder can be effectively improved, liver function damage can be relieved, and the intestinal barrier function can be effectively improved.

The invention relates to the field of microorganisms, and discloses a strain of lactobacillus acidophilus and application of the lactobacillus acidophilus in preparation of drugs for preventing and / or treating hyperlipidemia and hyperglycemia, the preservation number of the lactobacillus acidophilus is CGMCC No.33155, the strain can generate lactic acid, is resistant to acid and cholate, and has strong self-aggregation ability and hydrophobic ability. The strain provided by the invention can promote the decomposition of fat and sugar by degrading cholesterol and inhibiting the activity of alpha-glucosidase and alpha-amylase, can reduce the expression levels of blood sugar, blood fat and inflammatory factors of hyperlipidemia and hyperglycemia mice, and provides a new idea for preparing medicines for preventing and / or treating hyperglycemia and hyperlipidemia.

The invention relates to a biological preparation for improving quality of weever, the biological preparation comprises an injection and an edible agent, the injection comprises chemically modified chemically synthesized single-stranded small RNA, Rev-erbalpha protein and Per1 protein, and the edible agent comprises Clock core peptide 1, Clock core peptide 2 and taurine. Skeletal muscle development, liver lipid metabolism and generation of cell inflammatory factors of the weever are influenced by adding and injecting the feed, so that the quality of the weever is improved, intramuscular fat deposition is promoted, the taste and flavor of fish meat are improved, the energy reserve of the weever can be improved, and the culture cycle is shortened. The improvement of cell inflammatory factors shows that the disease resistance of the weever is improved, the survival rate in the breeding process is further improved, and the use amount of antibiotics is reduced. The method provides an important theoretical basis for improving fish meat quality in China and promoting high-quality, efficient and healthy development of aquaculture, and has a wide market prospect.

The invention relates to a wearable aptamer electrochemical sensor and a preparation method and application thereof, and belongs to the field of wearable sensors, and the preparation method comprises the following steps: 1) preparing an electrochemical sensing unit on a flexible substrate, including a TNF-alpha working electrode, a sodium ion working electrode and a pH working electrode; 2) preparing a microfluidic layer with a sweat detection channel; and 3) combining the flexible substrate with the microfluidic layer, and correspondingly fitting the electrochemical sensing unit with the sweat detection channel so as to monitor TNF-alpha, sodium ions and pH in sweat. By adopting the overall flexible design, the sweat TNF-alpha monitoring device is suitable for noninvasive and dynamic monitoring of human body inflammatory factors in a resting state, and the accuracy of sweat TNF-alpha monitoring can be improved; the non-invasive monitoring problem of high sensitivity, high specificity, high accuracy and high stability of sweat inflammatory factors in a non-motion state is solved, and the prepared electrochemical sensor can be attached to the surface of human skin and can be further applied to monitoring and management of inflammatory diseases and related fields.

The invention relates to the technical field of spermidine application, in particular to application of spermidine in preparation of medicine for preventing and treating doxorubicin-induced cardiotoxicity and myocardial aging. Cell proliferation experiments and mouse survival rate experiments show that spermidine can reduce death of myocardial cells and mice caused by adriamycin; a q-PCR experiment shows that spermidine can improve abnormal expression conditions of P53, P21, P16, BAX, TNF-alpha, IFN alpha, IL-10 and IL-18 in myocardial cells caused by adriamycin; a beta-galactosidase staining experiment shows that the spermidine can reduce the condition that the expression of a marker beta-galactosidase for myocardial cell senescence induced by adriamycin is increased; a Western Blot experiment shows that the spermidine can improve the condition that the P53 protein is abnormally increased due to the adriamycin; an Elisa experiment shows that spermidine can improve the condition that myocardial injury markers cTn-I and AST and inflammatory factors IL-1beta are abnormally increased due to adriamycin, and the myocardial injury markers cTn-I and AST and inflammatory factors IL-1beta are abnormally increased due to adriamycin. Therefore, spermidine can improve cardiotoxicity and myocardial aging induced by adriamycin.

The invention provides a micromolecular multifunctional information peptide and application thereof, and belongs to the technical field of active peptide raw material development. The sequence of the small-molecule multifunctional information peptide is VHPYVPR, the small-molecule multifunctional information peptide can be effectively combined with a downstream receptor of EGF, the EGF function is simulated, and meanwhile the situation that use of existing cell growth factor raw materials in the field of cosmetics is limited due to the molecular weight problem is avoided. Experiments prove that the micromolecular multifunctional information peptide provided by the invention can promote the repair of skin damage phenomena such as scratches, inhibit the release of inflammatory factors, inhibit the activity of beta-galactosidase and promote the generation of I-type, III-type and XVII-type collagen, shows excellent repair, soothing and anti-aging functions, is high in permeability and has a wide application prospect.

The invention belongs to the technical field of medicines, and particularly relates to a pyruvate-containing composite preparation as well as a preparation method and application thereof. The composite preparation containing pyruvate is prepared from the following components in percentage by mass: 0.02 to 0.04 percent of a compound A, 0.05 to 0.1 percent of pyruvate, 0.2 to 2 percent of sodium dihydrogen phosphate, 0.2 to 2 percent of disodium hydrogen phosphate, 0.7 to 0.9 percent of sodium chloride and the balance of water for injection, the structural formula of the compound A is as follows: # imgabs0 #. The compound A and pyruvate in the compound preparation interact with each other, so that lung inflammatory response can be inhibited, the level of inflammatory factors can be reduced, and lung edema can be relieved.

The invention provides copper-loaded duck-origin lactobacillus plantarum with high antibacterial peptide yield and application of the copper-loaded duck-origin lactobacillus plantarum. The preservation number of the provided copper-loaded duck-origin lactobacillus plantarum is CGMCC (China General Microbiological Culture Collection Center) NO.32835. The domesticated and bred duck-origin lactobacillus plantarum with high antibacterial peptide yield can tolerate high-concentration copper ions, the domesticated and bred lactobacillus plantarum can stably grow, and the contents of antibacterial peptide, extracellular protein, lipase, amylase, lactic acid, acetic acid and the like in the domesticated and bred lactobacillus plantarum strain are increased; and the amino acid composition is changed. The strain can convert inorganic copper into organic copper, the utilization rate of copper is increased, and growth and development of organisms are promoted. Moreover, small peptides and extracellular proteins in metabiotics generated after fermentation have the effect of effectively inhibiting harmful bacteria such as riemerella anatipestifer, escherichia coli, salmonella and staphylococcus of poultry, and are beneficial to eliminating intestinal inflammatory factors, maintaining intestinal health and improving the feed utilization rate.

The invention relates to a novel application of animal bifidobacterium subsp. Lactis BGI-N3, in particular to an application of the animal bifidobacterium subsp. Lactis BGI-N3 in preparation of a preparation for relieving skin allergy. The invention finds that the bifidobacterium animalis subsp. Lactis BGI-N3 has a very remarkable effect in the aspect of relieving skin allergy, and the effect is specifically shown as follows: in an atopic dermatitis animal model, the dermatitis score, scratching times and percutaneous water loss are remarkably reduced, and the atopic dermatitis skin tissue damage condition is relieved; the immune organ edema condition is improved, the levels of Th2 type cell factors and inflammatory factors IL-6, TNF-alpha and IFN-gamma related to dermatitis are reduced, and the immune balance of the body is recovered; expression of fibroin and endothelial protein in skin tissue is improved, and the skin barrier is repaired; the level of antibacterial peptide in skin tissues is recovered, and the antibacterial activity of the skin is enhanced. Meanwhile, compared with a traditional chemical medicine for treating atopic dermatitis, the animal bifidobacterium subsp. Lactis BGI-N3 has no side effects such as weight loss, immune organ atrophy, immune imbalance and the like.

The invention discloses an intestinal strain capable of effectively regulating uric acid metabolism, a microbial agent of the intestinal strain and application of the intestinal strain in uric acid reducing products, and belongs to the technical field of microorganisms. According to the enterococcus pluvialis ZQ205, the enterococcus pluvialis ZQ205 which is free of toxic and side effects and capable of effectively relieving and treating the hyperuricemia is found for the first time, and the collection number of the enterococcus pluvialis ZQ205 in the China Center for Type Culture Collection is CCTCC NO: M 2025844; the strain can significantly reduce the levels of uric acid, creatinine and urea nitrogen in serum of mice, can effectively reduce the levels of inflammatory factors such as IL-6 and TNF-alpha in serum of hyperuricemia mice, and can improve kidney injury caused by hyperuricemia.

The invention discloses application and an extraction method of prinsepia utilis royle polysaccharide, which comprises the following steps: extracting prinsepia utilis royle residues with 95% ethanol at 85 DEG C, filtering, extracting the residues with deionized water at 85-95 DEG C for 2-3 times, merging extracts, performing alcohol precipitation with 90%-95% ethanol at 2-6 DEG C, removing protein through a Sevage method, centrifugally collecting supernate, and freeze-drying to obtain the prinsepia utilis royle polysaccharide. And finally determining the content of the prinsepia utilis royle polysaccharide by using an anthrone-sulfuric acid colorimetric method, wherein the obtained prinsepia utilis royle polysaccharide is used for treating and improving skin lesion of psoriasis. Tests prove that prinsepia utilis royle polysaccharide can significantly improve psoriasis skin lesion, reduce inflammatory factor expression, enhance skin barrier function and increase expression of epidermal related protein, and is helpful for relieving symptoms of psoriasis patients and improving skin conditions.

The present invention belongs to the field of cosmetics detection, and discloses a method for evaluating the sun protection function of cosmetics and cosmetic raw materials and its application. This evaluation method uses a non-contact drug delivery device to test the sun protection effect of the test samples, and the test samples are finished cosmetics or diluted cosmetic raw materials; the tests include evaluating the in vitro sun protection efficacy by skin cells, evaluating the in vivo sun protection efficacy by the zebrafish embryo model, and result evaluation. This method evaluates the anti-aging effect of sun protection through the expression of β-galactosidase and / or the secretion of collagen in human dermal fibroblasts, and evaluates the sunburn prevention effect through the survival rate of human keratinocytes, DNA damage, or the secretion of inflammatory factors and the lethal / teratogenic conditions of zebrafish embryos, realizing multi-level and all-round evaluation of the sun protection efficacy. The method provided by the present invention is applicable to the evaluation of the sun protection function of various sun protection products, has accuracy and practicability, and provides an important technical basis for the research and development, quality control, and performance optimization of sun protection cosmetics.

The invention relates to the technical field of microorganisms, in particular to a bifidobacterium animalis subsp. Lactis and application thereof, and particularly provides a bifidobacterium animalis subsp. Lactis BX-BC08 which is beneficial for restoring the microecological balance of the intestinal tract and enhancing the immune barrier function of the intestinal tract. Experiments prove that the bifidobacterium animalis subsp. Lactis BX-BC08 disclosed by the invention can regulate an immune system and relieve excessive immune response, can reduce generation of inflammatory factors and relieve skin inflammation symptoms for autoimmune related diseases such as atopic dermatitis, is relatively good in tolerance to a human body, and greatly reduces adverse reaction of the human body.

The invention provides an anti-aging repair composition containing PDRN, and lipid nanoparticles and application thereof. The composition provided by the invention is prepared from polydeoxyribonucleotide, micro-current tetrapeptide-1 and palmitoyl tripeptide-5. According to the application, the polydeoxyribonucleotide, the micro-current tetrapeptide-1 and the palmitoyl tripeptide-5 are compounded for use, so that the composition has a synergistic effect in the aspects of promoting cell proliferation, inhibiting an intracellular ROS (reactive oxygen species) level and the like, and has remarkable antioxidant, anti-inflammatory, anti-aging, soothing and repairing effects. The PDRN-containing composition is prepared into the lipid nanoparticles, so that the stability of the PDRN-containing composition is improved, the permeability of the PDRN-containing composition on the skin surface is improved, the bioavailability of the PDRN-containing composition is improved, the cell survival rate and cell uptake are promoted, the expression of adenosine A2A receptors, the synthesis of collagen, angiogenesis and the inhibition of anti-inflammatory factors are promoted, and a remarkable anti-aging effect is achieved.

The invention relates to application of a tea exosome in preparation of a medicine for improving skin pigmentation, the tea exosome is creatively extracted and purified, and the tea exosome can effectively inhibit proliferation of melanoma cells and inhibit expression of melanin generation related protein by scavenging free radicals and inhibiting tyrosinase activity, so that the effect of improving skin pigmentation is achieved. The intracellular melanin removal is promoted. In a mouse in-vivo experiment, after the tea exosome is applied, it is observed that the skin epidermis thickness of a mouse is reduced, the dermis thickness is increased, collagen arrangement is tighter and more ordered, the content is increased, and meanwhile generation of inflammatory factors is reduced. The tea exosome can significantly increase the content of a melanin autophagy key factor LC3B and reduce the content of P62 to promote melanin autophagy, and has good percutaneous infiltration capacity, thereby playing a role in improving skin pigmentation.

The invention discloses a preparation method of a cell inflammatory factor adsorbent, which comprises the following steps: mixing styrene, divinyl benzene and hydroxyethyl acrylate, and adding a pore-foaming agent and mesoporous SiO2 nano powder to prepare a porous microsphere carrier; the preparation method comprises the following steps: mixing chitosan and poly (N-isopropylacrylamide) in an acetic acid solution to obtain a temperature-sensitive chitosan hydrogel precursor solution; the preparation method comprises the following steps: activating the surface of a porous microsphere carrier by using 1-ethyl-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide, mixing the activated porous microsphere carrier and a temperature-sensitive chitosan hydrogel precursor solution to obtain a water phase, and assembling, washing and drying in an oil phase to obtain the cell inflammatory factor adsorbent. Through the design of the core-shell structure and the addition of the mesoporous SiO2 nano powder, the temperature response characteristic and the recyclable characteristic are given to the cell inflammatory factor adsorbent, the adsorption capacity of cell factors is also improved, and a new solution is provided for physical adsorption of the cell inflammatory factors.

The present invention relates to a branched-chain fatty acid composition and its use in helping to regulate brain neuroimmunity, and belongs to the field of nutrient research. The branched-chain fatty acid composition provided by the present invention comprises C15:0 and C17:0, and more specifically comprises iso-C15:0, anteiso-C15:0, anteiso-C17:0 and iso-C17:0. The branched-chain fatty acid composition promotes the trend of LPS-induced microglia differentiation into M2 phenotype microglia, which is higher than the trend of promoting LPS-induced microglia differentiation into M1 phenotype microglia, and it also promotes the secretion of anti-inflammatory factors such as IL-4 and IL-10, and inhibits the secretion of pro-inflammatory factors such as NO, TNF-a, and IL-1β. Each branched-chain fatty acid in the branched-chain fatty acid composition has a synergistic effect, which can play a role in regulating brain neuroimmunity and maintaining the health of the central nervous system.

The invention relates to a probiotic agent for regulating an intestinal environment and an application of the probiotic agent. The bacterial strains in the probiotic agent for regulating the intestinal environment comprise a Bifidobacterium animalis subsp. Lactis C-1 bacterial strain and a Lactobacillus gasseri CKCC 1913 bacterial strain, and the bacterial strains in the probiotic agent for regulating the intestinal environment comprise a lactobacillus plantarum subsp. Lactis C-1 bacterial strain and a lactobacillus gasseri CKCC 1913 bacterial strain, a lactobacillus plantarum subsp. Lactis CKCC 1913 bacterial strain, a lactobacillus plantarum subsp. Lactis CKCC 1913 bacterial strain and a lactobacillus plantarum subsp. Lactis CKCC 1913 bacterial strain. A synergistic interaction relationship exists between the two bacteria, so that the intestinal micro-ecological environment can be regulated, the intestinal injury can be relieved and the intestinal barrier function can be improved by reducing the level of inflammatory factors, balancing the content of gastrointestinal regulatory peptides, promoting intestinal peristalsis and other action mechanisms. Therefore, the probiotic agent is suitable for preparing a preparation with the efficacy of preventing, improving or treating constipation and other inflammatory diseases.

The invention belongs to the technical field of gene engineering, and particularly relates to PDRN, a gene engineering vector, a cell, a preparation method and application thereof. The polydeoxyribonucleotide (PDRN) provided by the invention comprises a nucleotide sequence as shown in SEQ ID NO. 1; 1 or a partial fragment containing a nucleotide sequence shown in SEQ ID NO. 1; 1 or a sequence having 80% or more of homology with the nucleotide sequence as shown in SEQ ID NO. 1. The PDRN provided by the invention can effectively reduce and inhibit the expression level of inflammatory factors TNF-alpha and IL-6 and increase the wound healing rate of HUVEC cells, and is concentration-dependent. The PDRN provided by the invention has a good application prospect in preparation of anti-inflammatory or healing-promoting products.

The invention belongs to the technical field of biological medicine, and particularly relates to application of polypeptide in treatment of psoriasis. The amino acid sequence of the polypeptide provided by the invention is SEQ ID NO.1, so that the spleen-body ratio of a psoriasis mouse can be effectively reduced, and the inflammation level in the body can be reduced; the epidermal thickness of psoriasis mice and inflammatory factors in skin lesion tissues can be remarkably reduced, keratinocyte proliferation is reduced, and inflammatory factors released by inflammatory cells recruited to skin lesion positions are reduced. The polypeptide provided by the invention shows certain advantages in the aspects of relieving inflammatory response, promoting tissue repair and the like when being used for treating psoriasis. The invention provides a new method and means for treating psoriasis, and promotes the progress and development of medicine.

The present invention relates to the screening of functional probiotics and its application, and specifically provides a strain of Lactobacillus casei RH99 that produces high extracellular polysaccharides and relieves vaginitis, and its preservation number is CGMCC No.32108. The Lactobacillus casei can inhibit the growth of Gardnerella, Candida albicans, and Peptostreptococcus and their biofilm generation ability; the extracellular polysaccharides produced by it have a significant promoting effect on the biofilm formation of Lactobacillus casei itself. RH99 is inoculated into the vagina, and its strain, the metabolites (exocellular polysaccharides) produced, and the core monosaccharide (D-mannitol) can all inhibit the colonization of pathogenic bacteria and promote the colonization of lactic acid bacteria; and RH99 can also relieve vaginitis by inhibiting inflammatory factors. It can be seen that RH99 can inhibit the growth of pathogenic bacteria + inhibit biofilm formation + inhibit pathogenic bacteria adhesion + inhibit the level of inflammatory factors through key core metabolites, and realize the all-round inhibition of four-in-one.

The invention discloses lactobacillus mucilaginosus and application thereof in fermentation of sporoderm-broken ganoderma lucidum spore powder. The preservation number of the lactobacillus mucilaginosus is CGMCC (China General Microbiological Culture Collection Center) No.31453. The strain is separated and screened from vaginal secretions of healthy women at the childbearing age, is not hemolyzed, is sensitive to various common antibiotics and has strong lactic acid production capacity, and fermented supernate of the strain has strong growth inhibition capacity on escherichia coli, pseudomonas aeruginosa, staphylococcus aureus, salmonella typhimurium, shigella dysenteriae and gardnerella vaginalis and can be used for producing lactic acid. The compound has strong adhesion ability, growth inhibition ability and apoptosis promoting effect on human cervical cancer cells. When the strain is used for fermenting wall-broken ganoderma lucidum spore powder, the content of bioactive substances such as amino acids and derivatives thereof, fatty acids and steroids in fermentation liquor can be greatly increased, the strain has an obvious anti-inflammatory effect on DSS-induced mouse colitis, and the inflammatory factor level is remarkably reduced. The strain not only has significant application value in functional food and biological agents, but also opens up a new way for further development of ganoderma spores.

The invention belongs to the field of nano-enzyme preparation and biological application, and particularly relates to a preparation method and application of M2 type macrophage membrane coated FeMn diatomic nano-enzyme. The preparation method comprises the following steps: by taking nitrogen-doped carbon (BANT) as a carrier, loading Fe and Mn diatoms, synthesizing a novel nano material FeMnDA / BCNT diatomic nano-enzyme, extracting a macrophage membrane from natural macrophages, and coating the FeMnDA / BCNT nano material with the macrophage membrane to finally form M2 type macrophage membrane coated nano-particles, namely [MM] FeMnDA / BCNT. The [MM] FeMnDA / BCNT nano-enzyme prepared by the invention has good biological safety and simulated SOD and CAT enzyme activity, and the expression of inflammatory factors is reduced by removing excessive ROS (reactive oxygen species) in the cartilage cells induced by H2O2, so that the damage of oxidative stress to the cartilage cells is inhibited. The [MM] FeMnDA / BCNT nano-enzyme is not used for treating OA yet, so that a scientific basis is provided for further application and expansion of the diatomic nano-enzyme, and an effective strategy and a new thought are provided for treating osteoarthritis and chronic diseases related to oxidative stress in the future.

The invention discloses an anti-human inflammatory factor interleukin 6 monoclonal antibody and an application thereof. When the monoclonal antibody is prepared, an adopted immunogen is a human inflammatory factor interleukin 6 antigen, and an amino acid sequence of the human inflammatory factor interleukin 6 antigen comprises a sequence as shown in SEQ ID NO: 1. A group of monoclonal antibodies specifically combined with antigens is constructed based on the IL-6 antigens. The IL-6 monoclonal antibody has strong binding capacity with an IL-6 antigen in a clinical sample, has no cross reaction with other proteins, has good specificity and affinity compared with a commercial antigen, can be effectively applied to ELISA and a dry-type immunofluorescence method, has good detection linearity, precision, stability and accuracy, and can be widely applied to the field of immunoassay. The antibody can be combined with the antigen to serve as a detection reagent raw material combination, and has clinical detection advantages.

The invention discloses a preparation method and application of a sea cucumber exosome with an immune cell repairing function, and belongs to the technical field of biomedicine. The method comprises the following steps: feeding sea cucumbers by using a combined method of composite stress and an echinacea extract, extracting sea cucumber cells, and obtaining sea cucumber exosomes through ultracentrifugation; wherein the condition of composite stress is set as follows: the day and night temperature difference is not lower than 3 DEG C, and meanwhile, the dissolved oxygen content of the water body is adjusted to 75-90% of the dissolved oxygen content of the conventional feeding water body. The obtained sea cucumber exosome has a remarkable immune cell repairing function, and can effectively promote proliferation and activity recovery of immune cells, inhibit expression of inflammatory factors and improve the level of anti-inflammatory factors. The sea cucumber exosome prepared by the preparation method disclosed by the invention has an important application prospect in the research and development of immune repair drugs, and can provide a technical support for a novel immune regulation preparation in the field of biomedicine.