41 results about "Lactobacillus acidophilus" patented technology

The present invention discloses an anti-inflammatory Lactobacillus acidophilus composition for regulating immune responses, belonging to the fields of microbial technology and biomedicine. The composition comprises live lyophilized powders of Lactobacillus acidophilus, Lactobacillus plantarum, and Bifidobacterium longum, together with homologous postbiotic lyophilized powders of the same strains. By combining functionally complementary probiotics with their homologous postbiotics, a full-chain anti-inflammatory system is constructed, covering immune regulation, inflammation suppression, and intestinal barrier repair. The live bacteria enable long-term colonization and microecological regulation, while the postbiotics act rapidly, producing a synergistic effect. The composition is prepared solely from strain-derived fermentation products without exogenous additives, ensuring safety and purity, and provides a stable and efficient solution for inflammation relief and immune function optimization.

This invention belongs to the field of food fermentation technology, and specifically relates to a compound probiotic agent and its application in the preparation of fermented soy milk. This invention provides a compound probiotic agent composed of *Lactobacillus fermentum* NCPS LF01, *Pediococcus pentosaceus* NCPS PPE01, and *Lactobacillus acidophilus* NCPS LA01 in a volume ratio of 1-2:1:1, and applies this compound probiotic agent to the preparation of soy products, especially fermented soy milk. The beneficial effects of this invention are that fermented soy milk prepared using this compound probiotic agent has a rich flavor, and the total flavonoid and total phenolic content, as well as the in vitro DPPH free radical scavenging rate, are significantly superior to those of ordinary commercially available strains or other strains. In summary, this compound probiotic agent can effectively improve the retention level of active ingredients and antioxidant capacity of fermented soy milk, and has outstanding application value.

This invention provides a low glycemic index blueberry extract and its preparation method, belonging to the field of beverage processing technology. The preparation method includes the following steps: blueberries are pulped, then fermented using a compound strain of Lactobacillus acidophilus, Lactobacillus plantarum, and Bifidobacterium, filtered through a membrane filtration device to obtain a blueberry fermentation broth; blueberries are crushed, the pH of an ethanol solution is adjusted to 2-3 with citric acid, followed by ultrasonic extraction, centrifugation to collect the supernatant, and then vacuum distillation of the supernatant to obtain anthocyanin extract concentrate; the anthocyanin extract concentrate is freeze-dried to obtain anthocyanin extract dried product; the anthocyanin extract dried product and blueberry fermentation broth are mixed evenly at a mass-volume ratio of (0.2-0.3) g:1L, then maltitol is added to adjust acidity and sweetness, homogenized, filtered, and sterilized to obtain the blueberry extract. By using compound probiotic fermentation to reduce sugar content and combining it with alcohol extraction to enhance anthocyanins and improve efficacy, the resulting product has anthocyanin content ≥400 mg / L and a low GI.

The application provides a compound microbial inoculum for preparing pickled Chinese cabbage through fermentation of bean water and a fermentation method, and belongs to the technical field of fermented food technology.The compound microbial inoculum for preparing pickled Chinese cabbage through fermentation of bean water comprises the following bacterial species in percentage by mass: 40-60% of Lactobacillus plantarum, 20-35% of Lactobacillus acidophilus, 10-25% of Saccharomyces cerevisiae and 5-15% of Bacillus acidophilus.The pre-processed bean water is used as a fermentation substrate, and the compound microbial inoculum is inoculated to prepare pickled Chinese cabbage through fermentation, so that the fermentation period is shortened, the fermentation quality of the pickled Chinese cabbage is improved, the safety of the pickled Chinese cabbage is ensured, the pickled Chinese cabbage is endowed with special bean aroma, and the resource utilization of the bean water is realized.

The application discloses a probiotic composition and a production process of a probiotic fermented bread crumb, and belongs to the technical field of bread crumb processing. The probiotic composition is a microcapsule structure containing malt dextrin, arabic gum and heat-denatured whey protein isolate as wall materials, and lactobacillus acidophilus and lactobacillus rhamnosus as core materials. The structure can ensure that a small amount of live bacteria and metabolic products are slowly released through the micropores of the wall materials in a normal temperature fermentation environment, so that the free lactic acid bacteria are prevented from rapidly proliferating, excessively producing acid and competing with yeast for carbon and nitrogen sources in the initial stage of fermentation. The probiotic composition can provide nutrition for the yeast in the fermentation process, promote cell division and metabolic activity of the yeast, and the polydextrose, inulin and arabic gum in the microcapsule wall materials are hydrophilic colloids, which can form a uniform hydration network in the dough.

This invention discloses a kidney-tonifying and liver-nourishing compound probiotic Cistanche fermented drink and its preparation method, comprising the following steps: mixing and sterilizing Cistanche deserticola, Poria cocos, malt, functional components, and natural selenium-strontium mineral water to obtain a fermentation substrate; activating *Lactobacillus casei*, *Lactobacillus acidophilus*, *Lactobacillus rhamnosus*, and *Lactobacillus plantarum* strains respectively, centrifuging and freeze-drying, and then mixing them to obtain a compound probiotic powder; culturing the compound probiotic powder in MRS medium to obtain a compound probiotic liquid; inoculating the compound probiotic liquid into the fermentation substrate for fermentation, sterilizing, and obtaining the kidney-tonifying and liver-nourishing compound probiotic Cistanche deserticola fermented drink. This invention, through the innovative integrated formulation technology of combining Cistanche deserticola, malt, and Poria cocos as medicinal and edible ingredients, multifunctional factor compounding, and compound probiotic fermentation, prepares a kidney-tonifying probiotic Cistanche deserticola fermented drink with high active ingredients, clear efficacy, and excellent flavor.

The application discloses a cat oral cavity nursing probiotic composition and application thereof, and the probiotic composition comprises animal bifidobacterium and lactic acid bacteria, wherein the lactic acid bacteria is any one or a combination of multiple of salivarius, lactobacillus casei, enterococcus faecalis, lactobacillus rhamnosus, lactobacillus plantarum, lactobacillus acidophilus and lactobacillus reuteri; compared with single probiotic, the probiotic composition has better effect on oral cavity odor removal, can effectively improve cat oral cavity source halitosis, and can also reduce dental plaque formation, relieve gingivitis and dental caries, and can be applied to preparation of a cat oral cavity odor cleaner, and has good application prospect.

A probiotic composition for treating or preventing metabolic dysfunction-associated steatotic liver disease-associated hepatocellular carcinoma(MASLD-HCC) or colorectal cancer, comprises one or more microbiota selected from a group consisting of Lactobacillus helveticus, Lactobacillus plantarum, Lactobacillus rhamnosus GG, Lactobacillus paracasei, Lactobacillus acidophilus, Bifidobacterium breve, Bifidobacterium animalis subsp. Lactis, Streptococcus thermophilus or any combination thereof. The probiotic composition demonstrates improved therapeutic effects when combined with low-dose Sorafenib for MASLD-HCC and outperforms 5-Fluorouracil in colorectal cancer models.

This invention belongs to the field of microbial fermentation technology, and provides a method for increasing the polyphenol content of mulberry leaves through compound fermentation with lactic acid bacteria, as well as the fermentation product and its uses. Mulberry leaves are dried, pulverized, and sieved to obtain mulberry leaf powder; *Lactobacillus plantarum* ATCC8014, *Lactobacillus casei* ATCC334, and *Lactobacillus acidophilus* ATCC4356 are activated and mixed in a 1:1:1 ratio to obtain a compound bacterial solution; a fermentation substrate is prepared at a material-to-liquid ratio of 1:50 to 1:200 and sterilized, inoculated at 1% to 5%, and fermented at 33 to 41°C for 12 to 60 hours to obtain the fermented mulberry leaf product; the supernatant is separated from the solid-liquid mixture, filtered through a 0.22 μm filter, and freeze-dried to obtain the fermentation product. Under preferred conditions (1:100, 3.1%, 37.5°C, 49 hours), the total polyphenol content reaches 46.80 mg / g (gallic acid equivalent). The fermentation product can be used to prepare food or pharmaceutical compositions that improve indicators related to metabolic syndrome.

This invention relates to the field of microbial fermentation technology, specifically a method for preparing Rehmannia glutinosa through simultaneous fermentation with compound bacteria and a laxative effect. This method involves extracting four types of probiotics that meet national safety standards: Lactobacillus acidophilus, Bifidobacterium longum, Lactobacillus casei, and Saccharomyces cerevisiae, and then formulating a high-concentration compound bacterial agent according to the live bacteria ratio. The initial fermentation material is adjusted to a material-to-water ratio of 1:5 and a suitable pH is maintained. After autoclaving, the materials are inoculated with bacteria at a weight-to-volume ratio for biological culture. The entire system is placed in a 37°C environment for continuous constant-temperature static co-fermentation. Through specific enzymatic hydrolysis and metabolic pathways of multidimensional bacterial communities, the absolute content of the core active ingredient catalpol and soluble dietary fiber in the final product is significantly increased. This bio-fermentation process effectively eliminates the potential risk of spleen and stomach irritation and damage from traditional Chinese medicinal materials. The resulting dry powder raw material can be widely and safely used in the preparation of microecological functional foods or pharmaceutical preparations targeting constipation symptoms caused by intestinal dryness and fluid deficiency.

The present application belongs to the technical field of microbial fermentation, and specifically provides a compound probiotic fermented product of iris and a preparation method thereof. The method comprises the following steps: mixing iris raw materials with water, inoculating plant lactobacillus and lactobacillus acidophilus for fermentation, and obtaining the fermented product of iris. The product solves the technical pain points of high nitrite content and easy loss of nutritional components in traditional iris pickles, and significantly enhances the health care effect of the product with the help of probiotic fermentation. In addition, the compound strain and the fermentation substrate form a synergistic effect, which not only significantly improves the quality of the fermented product and the survival rate of the strain, but also ensures that the probiotic bacteria can successfully pass through the strong acid environment of the stomach, effectively colonize in the intestine, and then regulate the intestinal microecological balance, optimize the intestinal flora structure, improve the intestinal barrier function and metabolic level, and enhance the effect of promoting lung and regulating qi, and improving the physiological function of the lung.

The application discloses lactobacillus degrading hippophae rhamnoides malic acid and application thereof in preparation of hippophae rhamnoides juice, wherein one strain is classified and named as lactobacillus acidophilus (Lactobacillus acidophilus) Lactobacillus acidipiscis ) with a preservation number of CGMCC No. 37351, and one strain is classified and named as lactobacillus plantarum (Lactobacillus plantarum) Lactobacillus plantarum ) with a preservation number of CGMCC No. 37352. Fresh hippophae rhamnoides juice is mixed with sterile water according to a proportion, pasteurized after sugar degree is adjusted, cooled to room temperature, and pretreated; the lactobacillus is inoculated into the pretreated hippophae rhamnoides juice after being cultured to a logarithmic growth phase, and anaerobically cultured at 35±1 DEG C for 90-96 h. The lactobacillus screened in the application has a remarkable degradation effect on hippophae rhamnoides malic acid, can efficiently degrade hippophae rhamnoides malic acid, and meanwhile retains nutrition function and natural flavor, and can be used for preparation of hippophae rhamnoides juice.

The present application relates to the technical field of bioengineering, and particularly relates to a selenium-rich lactobacillus acidophilus and application thereof. The present application provides a selenium-rich lactobacillus acidophilus JGSLA09, and the strain is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 34272. The strain provided by the present application has the key ability of efficiently converting inorganic selenium into organic selenium and nano selenium, and is applied to the directional fermentation of asparagus tea extraction liquid, and a selenium-rich fermentation product with high selenium content and excellent antioxidant activity is successfully prepared. The technical scheme effectively breaks through the technical bottleneck of traditional selenium-rich tea, that is, the dependence on natural selenium-rich raw materials, low selenium element dissolution rate and low bioavailability, and provides a reliable microbial fermentation process path for developing safe, efficient and functionally synergistic new selenium-rich food.

The application belongs to the technical field of biological medicine, and particularly relates to an antimicrobial peptide and application thereof in preparation of products for preventing and / or treating oral diseases. The amino acid sequence of the antimicrobial peptide Perceptide-302 is shown as SEQ ID NO. 1. Experimental results of the application show that the polypeptide can effectively inhibit and kill oral pathogenic bacteria at a low concentration, the minimum inhibitory concentration (MIC) of the polypeptide on six kinds of bacteria, including Streptococcus mutans, Streptococcus sobrinus, Streptococcus sanguis, Streptococcus gordonii, Lactobacillus casei and Lactobacillus acidophilus, is 2.5 μg / mL-5 μg / mL, the minimum bactericidal concentration (MBC) is 5 μg / mL-10 μg / mL, and mechanism research is carried out on the most important clinical Streptococcus mutans. The application provides a new active ingredient and technical approach for developing a new type, efficient and safe oral disease prevention and treatment drug and product.

The application provides a silage fermentation additive and a preparation method and application thereof, and belongs to the technical field of silage feed. The silage fermentation additive comprises allicin and a compound microbial inoculum, and the compound microbial inoculum comprises lactobacillus acidophilus, lactobacillus plantarum and lactobacillus buchneri. The allicin is compounded with the compound microbial inoculum with a specific composition, and is used for preparing silage feed, so that the aerobic stability of the silage feed can be significantly improved, the growth of spoilage bacteria such as molds and yeasts can be effectively inhibited, the content of various mold toxins such as aflatoxin, zearalenone, T-2 toxin and vomitoxin can be reduced, the fermentation quality can be improved, the protein decomposition can be reduced, and the retention rate of dry matter and water-soluble total sugar can be improved. The application can completely replace chemical preservatives such as potassium sorbate and ammonium propionate, has the characteristics of naturalness, safety and high efficiency, and is suitable for the large-scale production of silage corn, fresh corn straw and other silage feeds.

This invention relates to the application of Lactobacillus acidophilus CL-01 in the preparation of products for relieving pollen-induced allergic rhinitis; the CL-01 strain is Lactobacillus acidophilus with accession number GDMCC No:64731. Lactobacillus acidophilus CL-01 strain. This invention develops a new use for Lactobacillus acidophilus CL-01, providing a research basis for its widespread application. It also develops a novel strategy for alleviating pollen-induced allergic rhinitis. This invention finds that Lactobacillus acidophilus CL-01 has a very significant effect in alleviating symptoms related to pollen-induced allergic rhinitis, specifically by significantly improving symptoms such as nasal mucosal lesions, nasal itching, sneezing, runny nose, asthma, and intestinal flora imbalance caused by pollen-induced allergic rhinitis.

This invention provides a composite composition containing nervonic acid and its use in brain nerve repair, belonging to the field of biotechnology. The invention uses nervonic acid and phosphatidylserine, combined with plant extracts fermented by a microbial concoction of *Lactobacillus acidophilus*: *Lactobacillus rhamnosus*: *Bacillus belyssae* = (2-3):1:1, to form a composite composition. This scientific formulation achieves synergistic effects from multiple components, improving neurological deficits, reducing cerebral infarction volume, decreasing the release of neurotrophic markers, and upregulating neurotrophic factor expression through multi-target synergistic effects. Furthermore, this synergistic effect depends on specific component ratios, providing crucial experimental support for the development of subsequent brain-protective functional compositions. This composition can be widely applied to the preparation of brain nerve repair drugs, functional foods, and dietary supplements, possessing promising industrialization prospects and clinical value.

The present application relates to a complex probiotic preparation containing Lactobacillus acidophilus WP285 and its use in the preparation of a product for inhibiting the formation of bird stone in pets, the strain in the complex probiotic preparation is composed of Bacillus coagulans Bacillus coagulans BC66 strain and Lactobacillus acidophilus Lactobacillus acidophilus WP285 strain. The present application finds that both BC66 strain and WP285 strain have excellent effect in inhibiting the formation of bird stone in pets, and they can synergistically enhance each other, the complex probiotic preparation can effectively reduce the pH value of the simulated stone formation environment and inhibit the formation and deposition of bird stone crystals in vitro; through pet clinical test, it is confirmed that the complex probiotic preparation can effectively and continuously adjust the urine pH value of susceptible cats to the weak acidic range which is not conducive to the formation of bird stone, and significantly reduces the number of bird stone crystals in urine.

The present disclosure relates to the field of microbial therapy, and specifically to a product for preventing and / or treating obesity and a metabolic syndrome. The product includes a Lactobacillus acidophilus strain JYLA-126 and a coating solution thereof. A bacterial solution of the L. acidophilus strain JYLA-126 is coated with a bacterial coating solution to better transport the bacterial solution to the intestinal tract to exert therapeutic effects. The L. acidophilus strain JYLA-126 coating solution is used for intragastric treatment of obesity mice induced by high-fat diet. Various indicators show that the L. acidophilus strain JYLA-126 coating solution may effectively regulate intestinal microflora and maintain intestinal health. The coating solution improves liver glucose metabolism, reduces liver oxidative stress, improves liver lipid metabolism, and alleviates a liver inflammation level. This coating solution improves white adipose accumulation and promotes brown adipose thermogenesis, thereby achieving effective treatment of obesity and metabolic syndrome in mice.

The application discloses a preparation method of lactic acid bacteria fermented foxtail chub jelly. The application uses foxtail chub natural gum to replace artificial thickening agent, uses cane sugar and milk to prepare a mixed substrate, uses Lactobacillus bulgaricus, Lactobacillus acidophilus and Streptococcus thermophilus to prepare a lactic acid bacteria preparation, and performs combined fermentation to produce organic acid to replace citric acid to promote coagulation, without adding preservatives, so that the functional jelly with pure naturalness, high nutrition and excellent flavor is prepared.

This invention discloses a lactic acid bacteria-fermented jujube juice and its application in antioxidation and lipid-lowering, belonging to the field of microbial fermentation technology. The preparation of the fermented jujube juice includes the step of fermenting jujube juice using a compound bacteria; the compound bacteria are composed of *Lactobacillus plantarum* LP90 and *Lactobacillus acidophilus* LA85, with an optimal ratio of 1:2; the jujube juice is obtained by juicing pitted jujubes. This invention systematically studies and optimizes the lactic acid bacteria fermentation process of jujube juice, addressing problems such as insufficient functional activity exploration, lack of precise optimization of fermentation process parameters, and low utilization rate of jujube pulp (a byproduct of jujube kernel processing) in existing jujube juice processing. Through strain selection, metabolomics mechanism exploration, and multi-objective process optimization using a BP neural network, the resulting fermented jujube juice exhibits significantly enhanced antioxidant properties and a marked lipid-lowering function.

The present application relates to the technical field of probiotic composition, and discloses an anti-helicobacter pylori probiotic composition for repairing gastric mucosa and regulating intestinal flora, which comprises 35-45 parts of Lactobacillus plantarum R202448 freeze-dried powder, 20-30 parts of Weizmannia coagulans BHE26 freeze-dried powder, 15-25 parts of Lactobacillus paracasei SMN-LBK freeze-dried powder, 10-15 parts of Lactobacillus acidophilus NCFM freeze-dried powder, 5-10 parts of prebiotic compound, 2-5 parts of gastric mucosa repair enhancer and 5-10 parts of auxiliary materials. Different probiotics are fermented separately in the present application, so as to avoid the nutritional competition and metabolic antagonism of mixed fermentation, and at the same time, a compatible metabolic environment between strains is constructed in advance through metabolic adaptation liquid, so as to ensure that the synergistic effect of each strain after compounding is not weakened.

The application discloses a kind of mixed lactic acid bacteria fermented sour pomegranate juice and preparation method thereof, belong to food processing technical field.The application uses sour pomegranate juice as raw material, adopts Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus fermentum three kinds of lactic acid bacteria according to optimized proportion mixed fermentation, is made into fermented juice after deployment, concentration, sterilization and the like process.The application significantly improves the polyphenol content and antioxidant activity of sour pomegranate juice by specific strain combination and its fermentation process optimization, improves flavor quality, electronic tongue analysis shows that umami is increased by 32%, and alcohol thickness is enhanced by 6%, GC-IMS analysis shows that characteristic aroma components such as hexanal and limonene are increased by 1.95 times and 1.45 times respectively.The application uses erythritol to replace sucrose to prepare low-sugar juice, and the product has bright color, sour and sweet taste, unique aroma, good nutritional value and market application prospect.

This invention discloses a biological agent suitable for squab growth, its preparation method, and its application, belonging to the field of feed technology. It comprises the following components by weight: 8-40 parts of microbial agent, 1-10 parts of additive, 1-10 parts of antioxidant, 0.5-5 parts of peptidoglycan, and 40-60 parts of montmorillonite. The microbial agent includes *Bifidobacterium animalis*, *Lactobacillus acidophilus*, *Lactobacillus paracasei*, and *Lactobacillus reuteri*. The additive includes at least one of yeast β-glucan and astragalus polysaccharide. The biological agent for squab growth of this application uses montmorillonite as a base material. Under the combined action of the microbial agent, additive, antioxidant, and peptidoglycan, it can regulate the intestinal health of breeding pigeons, improve their immunity, and increase immune factors in pigeon milk. This can improve the growth performance of squabs, reduce the culling rate of both breeding and squabs, thereby increasing economic benefits and showing broad application prospects.

The invention discloses a lactic acid bacterium agent for selenium-rich fermentation of rape bee pollen as well as a preparation method and application of the lactic acid bacterium agent, and belongs to the technical field of fermentation engineering. The lactic acid bacteria comprise one or a mixture of more of lactobacillus acidophilus subjected to selenium-rich culture, lactobacillus plantarum subjected to selenium-rich culture and lactobacillus rhamnosus subjected to selenium-rich culture; the preparation method comprises the following steps: inoculating one kind of lactic acid bacteria, adding sodium selenite for fermentation 2-12h after inoculating, or respectively inoculating lactic acid bacteria, adding sodium selenite for fermentation 2-12h after inoculating to obtain selenium-rich lactic acid bacteria, and mixing the selenium-rich lactic acid bacteria to obtain the lactic acid bacteria agent. The addition amount of the sodium selenite is 2 to 10 mg / L. The lactic acid bacterium agent is used for fermenting the rape bee pollen, so that the selenium-rich fermented rape bee pollen with a new health-care function can be obtained, the biological activity of the rape bee pollen is improved, the buying desire of consumers can be promoted, the additional value of the bee pollen product is greatly improved, and the market of the bee pollen product is further expanded.

The present application belongs to the technical field of nutritional health food, and particularly relates to a composition for repairing oral mucosa and a preparation method and application thereof. The composition comprises, by weight of the composition: 2%-80% of yeast beta glucan, 0.5%-50% of lactobacillus salivarius powder, 0.5%-50% of lactobacillus acidophilus powder, and 0.5%-50% of paracasei lactobacillus powder. The composition for repairing oral mucosa and the auxiliary materials are further prepared into a functional food in the form of tablets, liquids, soft candies or powders. In the functional food, the composition as the main component plays a role in repairing and preventing damage to the oral mucosa. Meanwhile, the composition and the auxiliary materials are dissolved under the action of oral saliva enzymes, thereby fully contacting the oral mucosa and pathogens and emitting a sweet and sour taste, so as to bring a pleasant sensory experience to the oral cavity.

The application discloses a red crawfish food-enticing fermented feed and a preparation method thereof, and belongs to the technical field of feed fermentation and food-enticing. The red crawfish food-enticing fermented feed is prepared by using a complex bacteria liquid system of Hansenula, Saccharomyces cerevisiae, Lactobacillus plantarum and Lactobacillus acidophilus, and a large amount of high-activity esters and acid-smelling food-enticing substances are generated through a double mechanism of "bacteria liquid culture first round ester production + continuous ester production in puffed material", and the food-enticing effect is further improved after synergistic action with conventional food-enticing agents. The red crawfish food-enticing fermented feed provided by the application can significantly shorten the feeding response time of red crawfish, improve the feeding rate, especially in the low-temperature environment of 8-20 DEG C in autumn, winter and early spring, and the effect is outstanding. Meanwhile, the red crawfish food-enticing fermented feed can significantly improve the weight gain rate, survival rate and intestinal digestive enzyme activity of red crawfish, reduce the ammonia nitrogen and nitrite content of the aquaculture water, solve the problems of low feeding of red crawfish, low feed utilization rate and water pollution in the low-temperature period, provide a feed solution with stronger food-enticing effect and green high efficiency, and has a wide application prospect.

The application discloses pet intestinal tract health care products containing lactobacillus acidophilus and inulin and a preparation method, and belongs to the technical field of biotechnology.The application realizes high synergy among components through ingenious structural design and preparation process optimization, ensures that the lactobacillus acidophilus can effectively pass through the harsh environment of gastric acid and bile salt, and is efficiently released and planted in the target intestinal segment, and the inulin can accurately provide prebiotic effects, so that the intestinal microecological balance of pets is significantly improved.The technical scheme provided by the application is a system-level innovation, unique double-layer embedding and slow-release technology, accurate synergy mechanism of probiotics and prebiotics, and a palatable matrix constructed in a scientific manner, which jointly constitute a highly original and non-obvious technical breakthrough, and provide an innovative solution which is efficient, stable and easy to be accepted by pets for the pet intestinal tract health care field.

The application provides a probiotic composition capable of promoting proliferation of intestinal flora, and a preparation method and application thereof, and relates to the technical field of microorganisms. The probiotic composition comprises the following raw materials: probiotics and prebiotics; the probiotics are a mixture of lactobacillus crispatus, lactobacillus acidophilus and animal bifidobacterium with a mass ratio of (0.1-2):(2-4):(5-7); and the prebiotics are a mixture of xylo-oligosaccharide, oat beta-glucan, arabinoxylan and 2'-fucosyllactose with a mass ratio of (3-5):(1-2):(3-4):(0.1-1). The application significantly promotes proliferation of beneficial flora in the intestines through specific probiotics and specific prebiotics, and interaction between components.

This invention relates to a L. casei / KGM synbiotic comprises: Lactobacillus, its bacterial lysate, and fermentation products, wherein the Lactobacillus is selected from one or more of the following group: Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus rhamnosus, and Lactobacillus paracasei; a polysaccharide; preferably, the polysaccharide is konjac glucomannan; and a wall material; wherein the Lactobacillus or its bacterial lysate and fermentation products are coupled with the polysaccharide, and the wall material encapsulates the coupled material. The present invention provides... L. casei / KGM Synbiotic is easy to use as a poultry feed additive and can effectively treat or prevent intestinal diseases such as enteritis in poultry.