2152 results about "Lactobacillus acidophilus" patented technology

A preparation method of a ferment Chinese Traditional Herbs for feeding belongs to the production field of the feed additives. The technical proposal provided by the present invention is that one or various intestinal probiotics is inoculated on a cultivation matrix containing extracts of Chinese Traditional Herbs, and undergoes ferment cultivation in a proper condition. The probiotics include Lactobacillus acidophilus, Bacillus subtilis, and candida utilis, etc, and the selected Chinese Traditional Herbs include astragalus, atractlylis lancea formalyrata, codonopsis pilosula, pericarpium citri reticulatae, and atractylis atractylis ovata, etc. The ferment Chinese Traditional Herbs for feeding provided by the present invention used to breed the livestock and the poultry is capable of preventing and treating epidemic diseases and improving the growth of the animal. The biological ferment of the water extract of Chinese Traditional Herbs by the probiotics is utilized to produce the elements with higher activities, thereby improving the absorption, transportation, metabolism, activation and medical efficacy of the Chinese Traditional Herbs in human body.

A mixture of at least 6 species of lactic acid bacteria and / or Bifidobacteria is disclosed for use in bakery and medical field. The preferred mixture comprises Streptococcus thermophilus, Bifidobacterium infantis, Bifidobacterium longum, Bifidobacterium breve, Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus casei, Lactobacillus delbrueckii subsp. bulgaricus. Said mixture is useful for a sourdough, a leavening composition. Baked goods and other food products obtained therefrom are disclosed. These goods have low or no gluten content and are suitable for the integration of the diet of a subject suffering from celiac disease, for decreasing the risk of allergies due to wheat flour albumins and globulins, for the treatment of schizophrenic symptoms, in the preparation of products for enteric diet.

Stress-related nucleic acid molecules and polypeptides and fragments and variants thereof are disclosed in the current invention. In addition, stress-related fusion proteins, antigenic peptides, and anti-stress-related antibodies are encompassed. The invention also provides recombinant expression vectors containing a nucleic acid molecule of the invention and host cells into which the expression vectors have been introduced. Methods for producing the polypeptides and methods of use for the polypeptides of the invention are further disclosed.

The invention relates to micro-ecological Chinese medicine preparation for livestock and poultry by adoption of multi-strain fermentation production and a fermentation method. The compositions of traditional Chinese medicines in a culture medium of the fermentation preparation include: radix astragali, radix codonopsitis, Atractylodes macrocephala, Poria cocos, liquorice, medicated leaven, hawkthorn, angelica, Chinese rhubarb, Scutellaria baicalensis, radix isatidis, cordate houttuynia, sicklesenna seeds, Schisandra chinensis, Gynostemma pentaphylla, phellodendron, dried orange peel, radix bupleuri, curcuma, honeysuckle, Chinese gall, purslane and Quisqualis indica. The fermentation method is as follows: a multi-strain solid state fermentation strain is adopted for fermentation; bacillus subtilis, bacillus natto, bacillus licheniformis, beer yeast, Candida wtilis, Aspergillus niger, lactobacillus plantarum, Lactobacillus acidophilus and bifidobacteria are purified and subjected to anaerobic fermentation under the conditions of a rotating speed of 180 revolutions per minute at a temperature is 32 DEG C for 48 to 72 hours till the pH value reaches 4.0. The micro-ecological Chinese medicine preparation has the advantages that the micro-ecological Chinese medicine preparation uses probiotics to ferment different Chinese medicine compositions, and is micro-ecological Chinese medicine preparation which completely replaces antibiotics and has the advantages of no residual medicine, disease prevention, growth promotion and low cost.

The invention relates to a micro-ecological preparation and the application thereof, in particular to a micro-ecological preparation containing a variety of probiotics and the application thereof. The micro-ecological preparation contains any three or four of the components including CGMCC No. 2383 bacillus licheniformis powder, bacillus subtilis powder, CGMCC No. 2386 enterococcus faecalis powder, lactobacillus acidophilus powder, and CGMCC No. 2388 saccharomyces cerevisiae. The micro-ecological preparation has content of live bacteria, has the adversity resistance such as gastric acid resistance, bile salt resistance, high-temperature resistance, common antibiotic resistance, and the like and the probiotic functions of producing acid and enzyme and resisting pathogenic bacteria. The variety and the proportion of the probiotics and carrier can be determined according to different kinds of animals and different animal growth phase. The micro-ecological preparation can improve the feed utilization efficiency, increase the yield of meat, eggs and milk, promote the growth of the animal, improve the immunity and the disease resistance of the animal, replace the antibiotic and improve the quality of the animal product.

The invention discloses a micro-ecological fruit-vegetable enzyme preparation method. The method comprises the steps of culturing 4 to 6 of six probiotics including lactobacillus acidophilus, bacillus coagulans, bacillus natto, saccharomyces cerevisiae, enterococcus faecium and bifidobacterium in a culture medium comprising ingredients such as brown sugar, liquorice and fresh sweet potato, and then culturing the probiotics through a diluted culture liquid containing brown sugar and liquorice in an activating manner to prepare a micro-ecological preparation, wherein the prepared micro-ecological preparation is a fermenting agent which is used for fermenting fruit pulp of fresh fruit and vegetable and traditional Chinese herbs. The microorganism enzyme in the ecological preparation and the natural vegetable enzyme in fruits, vegetables and Chinese herbs are compounded into a composite enzyme supplement by utilizing a micro-ecological fermentation technology, i.e. the micro-ecological fruit-vegetable enzyme. The micro-ecological fruit-vegetable enzyme can be made into a liquid type or a solid type (powder). An ideal effect is achieved in the fields such as medicine, healthcare and beauty.

The invention relates to a composite probiotics feed additive containing lactic acid bacteria. The composite probiotics feed additive is prepared by spraying, freezing, drying and compounding 40-60% of lactobacillus acidophilus, 15-20% of lactobacillus amylophilus, 5-10% of lactobacillus salivarius, 5-10% of streptococcus thermophilus, 5-10% of bacteroides thetaiotaomicron and 5-10% of glucose. All strains are separated from pig intestines chime. With the consideration of each part of an intestinal canal of animal, the lactic acid bacteria which produce lactic acid of the front section of the intestinal canal are combined with the bacteroides thetaiotaomicron which dissolve cellulose and polysaccharide of the rear section of the intestinal canal, the proportion among the bacteria is similar to the composition of corresponding bacteria in the intestinal canal of a healthy animal, so that the respective advantages can be developed well, the health of the animal is improved further, the feed digestibility is improved, and the production performance of the animal is improved.

A dual-effect microbial feed additive for ruminant is prepared through pulverizing corn, steaming, liquefying, saccharified, inoculating lactobacillus acidophilus and saccharomyce cerevisiae, fermenting to obtain living bacterium preparation, culturing bacillus subtilis, aspergillus oryzae and aspergillus niger in the culture medium prepared from corn, bran and soybean dregs, fermenting to obtain composite enzyme preparation, and proportionally mixing said living bacterium preparation with said composite enzyme preparation. It can promote the growth and health of ruminant and increase the utilization rate of feed.

The invention provides a preparation method for feed without antibiotics. Feed without antibiotics is complete pig feed without antibiotics, which is prepared from alkali protease and fermenting mixing feed of wholesome funguses and contains the wholesome funguses, a complex enzyme, complex organic acid and other microbe metabolites. The preparation method comprises the preparation steps: weighingthe raw materials of the fermenting feed: corn flour, bean dregs, fermenting bean dregs, fish meal and whey mist which are uniformly mixed; weighing immune polysaccharide, edible salt, calcium hydrophosphate, calcium carbonate, lysine, methionine, various vitamins and various minerals which are uniformly mixed; preparing wine making yeast, alkyl producing pseudofilament yeast, lactobacillus acidophilus, bacillus subtilis and bacillus licheniformis which are uniformly mixed; uniformly mixing all the raw materials and the funguses; adding water to the feed; bagging; sealing; fermenting until nogas is generated; discharging air; sealing again; sealing an outer packing bag and labeling to obtain a finished product. The feed without antibiotics prepared by the method has the advantages of good mouth feel, high nutritious value, easy assimilation and wide market prospect.

The invention discloses a fermented feed additive, a preparation method and application thereof. The fermented feed additive is prepared by fermenting mixed strains and mixed auxiliary materials consisting of monosodium glutamate protein, bean pulp, bran, cottonseed meal, corn fibers, rice bran and powdered rice hulls, wherein the mixed strains are obtained by mixing activated strains of lactobacillus acidophilus, lactobacillus planetarium, lactobacillus casei, saccharomyces cerevisiae, bacillus subtilis and bacillus licheniformis in a proportion of (1-5): (1-5): (1-5): (1-5): (1-5): (1-5); and the dosage of the fermented feed additive is 5 to 100 percent of the weight of the animal feed when used for the animal feed. According to the fermented feed additive, the bean pulp, the bran, the cottonseed meal and other raw auxiliary materials are sufficiently utilized, so that the food consumption in the animal feeding process is reduced; the fermented feed additive can be used to partiallyor completely replace the animal feed, and antibiotic medicaments can be reduced or prevented from being used; and no medicament is left, so that the feeding cost of farmers is reduced.

A vaginal treatment composition that employs a therapeutic agent to inhibit and / or treat vaginal infection is provided. The therapeutic agent is capable of inhibiting and / or killing Gardnerella (e.g., Gardnerella vaginalis), Candida (e.g., Candida albicans), and / or Trichomonas (e.g., Trichomonas vaginalis) pathogens. Desirably, such antimicrobial efficacy is achieved without substantially inhibiting the growth of Lactobacillus acidophilus. For instance, sugars and / or sugar alcohols may be employed in the present invention as a therapeutic agent for inhibiting and / or treating vaginal infection. In one particular embodiment, D-xylitol is used as the therapeutic agent.

The invention discloses a method for producing a complex micro-ecological preparation with microbial agents and enzyme. According to the action principles and characteristics of every microbial strain, the method adopts scientific combination and adds enzyme preparations and acidifier to enable microorganisms in the product to grow rapidly and take dominance in fermented feed and animal bodies so as to reduce the growth opportunity of miscellaneous bacteria. The preparation comprises the following components: bacillus subtilis, lactobacillus plantarum, candida utilis yeast, lactobacillus acidophilus, protease, amylase, cellulase, xylanase, mannanase, galactosidase, pectinase and citric acid. As all strains of the product adopt liquid deep fermentation culture, the non-polluting property of the product is guaranteed. Liquid microbial agents ensure that the number of bacterial colonies of the product is more than 100 million and the product is free from the pollution of other miscellaneous bacteria, while solid microbial agents ensure that the number of bacterial colonies of the product is more than 1 billion and the proportion of miscellaneous bacteria to effective bacteria is not more than 0.0001. The preparation contains bacillus, lactic acid bacteria and yeast, can apply to the processes of making green-yellow storage feed, fermenting feed, soaking feed, and the like, can be directly added to feed for feeding, and can improve the growth performance of ruminant animals, poultry, pigs, aquatic products and special animals.

The invention provides an intestinal tract micro-ecological preparation and its preparing process, wherein each 100g of the product contains bifidobacteria 1.0-20.0g, Lactobacillus acidophilus 1.0-25.0g, Streptococcus thermophilus 1.0-25.0g, Bulgarian subspecies of Lactobacillus fermentum 1.0-25.0g, saccharomycete 1.0-35.0g, and right amount of isomaltose hypgather, fructooligosaccharide, and many kinds of vitamins.

The invention relates to a high-efficiency ferment for fermenting bean pulp, belonging to the biologic enzymolysis fermentation technical field. The high-efficiency ferment is formed by mixing bacillus subtilis, bacillus licheniformis, lactobacillus acidophilus, lactobacillus casei, saccharomyces cerevisiae and candida utilis. The fermentation technology comprises the following steps: crushing the bean pulp and corn; activating the strains; uniformly mixing the bean pulp and the fermentation liquor using a mixer; packing the mixed bean pulp in fermentation bag; fermenting the packaged bean pulp and processing fermented bean pulp: drying-crushing-detecting-packaging-finished product. The technology is simple and the product quality is stable and the content of the anti-nutrient factor in the bean pulp is eliminated and obviously reduced, therefore the bean pulp is a safe plant protein feeder for infant animal with balanced nutrient, good quality and low price and help for health growth of the infant animal, in replace of animal protein feeder of bone powder, fish powder or the like.

The invention discloses a microbial fermentation antibiotic-free feed, which comprises a batch and a composite fermentation material. The batch consists of the following raw materials in part by weight: 30 to 50 parts of corn, 20 to 40 parts of bean pulp, 5 to 20 parts of cotton seed meal, 2 to 5 parts of calcium hydrophosphate, 0.2 to 0.4 part of salt, 0.1 to 0.2 part of composite trace element, and 0.2 to 0.4 part of composite vitamin; and the composite fermentation material consists of the following fermentation materials in part by weight: 30 to 70 parts of lactobacillus acidophilus fermentation material, 10 to 20 parts of bacillus subtilis fermentation material, 20 to 35 parts of saccharomyces cerevisiae fermentation material, 1 to 3 parts of trichoderma longbrachiatum fermentation material, 1 to 3 parts of aspergillus niger fermentation material, and 1 to 3 parts of aspergillus oryzae fermentation material, wherein the weight ratio of the batch to the composite fermentation material is 96-92:4-8.

The invention discloses a preparation method of freeze dried microbial powder of Lactobacillus acidophilus, which comprises the technical processes of fermented cultivation of Lactobacillus acidophilus, separation of a fermented fluid, emulsification of microbial soil and vacuum freeze drying of an emulsified solution. The preparation method is characterized in that the strain fermented cultivation comprises the steps as follows: a lactobacillus strain is inoculated into a fluid culture medium in a triangular flask, cultivated at the temperature of 30 to 40 DEG C for 10 to 30 hours, inoculated into a large fermentation flask and fermentation cultivated at the temperature of 35 to 40 DEG C for 10 to 30 hours, and sterilized at the temperature of 115 DEG C for 15 minutes; and fermentation and augmentation cultivation is carried out for 10 to 30 hours, the pH value of the fermented fluid is 5.5 to 6.5, and OD600 is over 2.0; the vacuum freeze drying of the emulsified solution comprises the steps: the emulsified solution is pre-frozen at the temperature of minus 60 to minus 40 DEG C for 1 to 5 hours, and then vacuum frozen and dried at the temperature of minus 60 DEG C and with the vacuum degree of 1 to 8 Pa for 10 to 30 hours. The freeze dried microbial powder of Lactobacillus acidophilus with the total plate count of 1.0 multiplied by 10 cfu / g can be preserved for 18 months at the temperature of minus 18 DEG C with unaffected liveness and stability.

The invention relates to a feeding addition agent for microorganism viable bacteria and a preparing method thereof. The addition agent consists of 1.5-2.5 parts of bacillus coagulans viable bacteria powder, 0.5-2.0 parts of lactobacillus acidophilus viable bacteria powder, 1.0-2.5 parts of lactobacillus plantarum viable bacteria powder, 0.5-2.5 parts of bacillus natto viable bacteria powder, 0.5-1.5 parts of candida utili viable bacteria powder and 2-4.5 parts of aspergillus niger viable bacteria powder. Each gram of the content of viable bacteria is as follows: bacillus coagulans is not less than 1.0*10cfu / g; lactobacillus acidophilus is not less than 1.0*10cfu / g; lactobacillus plantarum is not less than 1.0-10cfu / g; bacillus natto is not less than 1.0*10cfu / g; aspergillus niger is not less than 1.0*10cfu / g; and candida utili is not less than 1.0*10cfu / g. The invention has the advantages that the composition is reasonable, the preparation method is scientific, the usage scale is wide, the operation is simple and easy to master, and the cost is low. In addition, the invention can be used as the feeding stuff agent for various poultries, thus increasing the use ratio of the feeding stuff addition agent and strengthening the anti-disease ability of the poultries.

The invention provides a method for preparing an acid organic fertilizer by orientation fermentation of an organic waste. The method is characterized by comprising the following specific steps of: mixing bacillus subtilis, bacillus licheniformis, bacillus pumilus, saccharomyces cerevisiae, acetobacter aceti, trichoderma, aspergillus oryzae, nocardia and geotrichum candidum to obtain a first composite microbial bacteria agent; mixing the bacillus subtilis, the bacillus licheniformis, the saccharomyces cerevisiae, bacillus coagulans, lactobacillus acidophilus, lactobacillus plantarum, lactobacillus casei, pediococcus pentosaceus, the aspergillus oryzae, mucor racemosus and the nocardia to obtain a second composite microbial bacteria agent; adding the first composite microbial bacteria agentinto the organic waste and performing aerobic fermentation so as to form an intermediate fermentation product; and adding the second composite microbial bacteria agent into the intermediate fermentation product and performing anaerobic fermentation so as to form the organic fertilizer. The method has the advantages of capability of improving saline and alkaline lands, suitability for cultivation of acidophilous crops, effect of performing micro-ecological remediation on continuous cropping soil and low cost.

The invention relates to a method for preparing a highland barley-wheat germ deep processing product, in particular to a process for preparing highland barley-wheat germ biological fermented milk, which solves the problems that the highland barley-wheat germ deep processing product has a single form and that nutrients cannot be absorbed by a human body completely. Lactobacillus bulgaricus, streptococcus thermophilus and lactobacillus acidophilus are inoculated according to a certain ratio, and a beta-glucan functional factor, dietary fibers, trace elements, microorganisms and the like of highland barley, and the nutrients such as high-quality plant proteins, vitamin E and the like of the wheat germ are utilized. The method mainly comprises the steps of: the compounding of the highland barley and the wheat germ, grinding into thick liquid, enzymolysis, the addition of milk and lactic acid fermentation. The prepared highland barley-wheat germ lactobacillus beverage is rich in proteins, lactic acid bacteria, beta-glucan, various trace elements, vitamins and the like.

The invention relates to a method for fermentation production of a poultry bacterial agent by multi-bacteria miscible liquid, wherein aerobic bacteria, namely Bacillus subtilis, Bacillus licheniformis, bacillus natto, beer yeast, Aspergillus niger and Aspergillus oryzae are cultured in a shaking table according to different culture mediums, so as to culture a mother seed solution; simultaneously Lactobacillus acidophilus, bifidobacteria and enterococcus faecalis are subjected to anaerobic culture by utilization of Kille flasks, and a mother bacterial solution of photosynthetic bacteria is cultured by a Kille flask under the condition of illumination; the prior stock solution is inoculated into a seed tank for anaerobic culture and fermentation according to 4 percent of the inoculum concentration, and the fermentation time is between 48 and 60 hours; and the cultured seed liquid is inoculated into a productive tank for anaerobic culture and fermentation according to 10 percent of the inoculum concentration, the fermentation time is between 60 and 72 hours, and the fermentation end point is reached when the pH value is reduced to 4.0. The method has the advantages that the microscopic examination viable count of the bacterial agent is more than 5 billion per milliliter, so that the bacterial agent is safe and nontoxic, thereby not only improving the disease resistance of poultry but also promoting the quick growth of the poultry, reducing the feed-meat ratio and improving the quality of meat, eggs and milk.

The invention belongs to the technical field of feed micro-ecology and relates to a composite micro-ecological preparation as well as a premixed material and an application of the preparation in a feed additive. The preparation contains Lactobacillus acidophilus powder and any two, three or four bacterium powders selected from Bacillus licheniformis, Bcillus pumilus, enteroccus and Bcillus subtilis; the preparation has high live bacteria content and low moisture content, has stress tolerance properties such as gastric acid resistance, cholate resistance, high temperature resistance, common antibiotics tolerance and the like and has the probiotic functions of producing acid and enzyme, inhibiting pathogenic bacteria and the like. By using the composite micro-ecological preparation of the invention, the utilization rate of the feed can be improved, the yields of meat, eggs, milk and the like are increased, the growth of animals is promoted, the immunity and disease-resistant capability of an animal organism are improved, and partial preparation can be used for replacing antibiotics, thereby changing the quality of an animal product.

A Lactobacillus plantarum BB9 capable of adhering to gastrointestinal tract and cholesterol removal is isolated from fruits and exhibits high BSH activity. In-vitro tests demonstrate that Lactobacillus plantarum BB9 has good acid and bile tolerance, and strong ability to adhere to intestinal cells. In-vivo tests show that hamsters fed high cholesterol diets added with BB9 strain have cholesterol and triglycerides in blood and liver effectively reduced, and their HDL-c / LDL-c ratios in blood are significantly higher than those of hamsters fed Lactobacillus acidophilus ATCC 43121 strain. It is hoped that the excellent acid and bile tolerance and intestinal adherence of the lactobacillus strain provided herein could produce cholesterol-lowering effect in humans.

The invention relates to a microbiological composite bacterial inoculum and a preparation method thereof, in particular to the microbiological composite bacterial inoculum used for preparing a biological bacterial fertilizer. The microbiological composite bacterial inoculum is prepared by culturing Rhodop seudanonas palustris, Candida utilis, Lactobacillus acidophilus, Bacillus subtilis and Streptomyces microflavus var. lactosus which are disclosed in a culture catalogue of the China Center for General Microbiology Culture Collection (CGMCC), wherein the serial number of the Rhodop seudanonas palustris is 1.2352; the serial number of the Candida utilis is 2.1180; the serial number of the Lactobacillus acidophilus is 1.1854; the serial number of the Bacillus subtilis is 1.1630; the serial number of the Streptomyces microflavus var. lactosus is 4.0891; and the weight ratio of the Rhodop seudanonas palustris to the Candida utilis to the Lactobacillus acidophilus to the Bacillus subtilis is 1:0.5:0.5:0.1. When used for preparing the biological bacterial fertilizer, the composite bacterial inoculum can effectively decompose and release crude fibers and other nutrient components in straws and livestock manure; and the prepared biological organic fertilizer can condition the soil, increase the activity rate of microorganisms in the soil, avoid soil hardening, improve the permeability of the soil, play a role in production increasing, and effectively solve the problem of the area source pollution of agricultural wastes in rural areas.

The invention provides a method for preparing edible bacteria synbiotic microcapsules. The method comprises the following steps: firstly, zymotic fluids of four strains of probiotic bacteria, namely Lactobacillus acidophilus, lactobacillus plantarum, lactobacillus casei and lactobacillus rhamnosus are prepared, then supernatants are removed after the speed of 5000r / min, and bacterial sludges are embedded for standby. 10g of each bacterial sludge of the probiotic bacteria to be embedded and 4g of edible bacteria Agaricus blazei powder with the fineness of 200 meshes are mixed with 40g of mixed colloid containing 2.5 percent of sodium alginate and 0.5 percent of flaxseed gum respectively; the first stirring is performed for 20min at a rotating speed of 200 r / min; the mixture is poured into a triangular flask which contains 1.5 times of volume of edible oil; the second stirring is performed for 20min at a rotating speed of 400r / min; a 1.5 percent CaCl2 solution is added into the mixture to be calcified for 3h; sterilized physiological saline is used to wash gel beads for three times; the gel beads are placed into a 0.6 percent chitosan solution with a pH value of 5.5 to perform a film-forming reaction for 15min, taken out, washed by the sterilized physiological saline and then placed into a citric acid solution with the concentration of 55mM to be liquefied for 30min; and wet microcapsules are obtained after the washing of the sterilized physiological saline, collected by centrifugating for 10min at a rotating speed of 150r / min, precooled, and finally freeze-dried to obtain the microcapsules.

The invention belongs to the technical field of food with homology of medicine and food, and particularly relates to a preparation method of rose ferment. The method comprises the steps of separating petals, sieving, protecting color, carrying out superfine grinding, carrying out fermentation pretreatment, planting bacteria for the first time, planting bacteria for the second time, planting bacteria for the third time, finely filtering and feeding collagen to obtain rose ferment liquid. According to the method, probiotic strains which are good for the intestinal tract of a human body are selected for use, and the fermentation temperature is strictly controlled, so that the loss of effective components in roses can be effectively reduced, and the activity of the rose ferment is maintained to the utmost extent; the dominant bacteria are implanted for three times, so that the growth of other bacteria can be effectively inhibited, and the product quality is stable; lactobacillus acidophilus, lactobacillus thermophilus and lactobacillus plantarum are selected, have symbiosis performance and can be planted in the intestinal tract after being fermented in a mixed way; furthermore, the comprehensive activity of ferment products can be better improved. The prepared rose ferment has a certain prevention function for chronic diseases such as heart disease and liver disease, and has the effects of soothing the liver and activating the spleen. After being eaten for a long time, the rose ferment also has the functions of smoothing the skin, whitening the skin and beautifying the face.

Cell wall, cell surface and secreted protein nucleic acid molecules and polypeptides and fragments and variants thereof are disclosed in the current invention. In addition, cell wall, cell surface and secreted fusion proteins, antigenic peptides, and anti-cell wall, cell surface and secreted antibodies are encompassed. The invention also provides recombinant expression vectors containing a nucleic acid molecule of the invention and host cells into which the expression vectors have been introduced. Methods for producing the polypeptides of the invention and methods for their use are further disclosed.

The invention discloses a deodorant special for livestock and poultry farms and application thereof, wherein the deodorant is composed of the following strains: 106-108 lactobacillus acidophilus per milliliter, 107-109 lactobacillus reuteri per milliliter, 104-106 bacillus polymyxa per milliliter, 106-108 bacillus lentus per milliliter, 106-109 bacillus coagulans per milliliter, 104-106 enterococcus faecalis per milliliter, 107-109 enterococcus faecium per milliliter, 104-107 candida rugosa per milliliter, 104-107 candida valida per milliliter, 104-106 pseudomonas farinofermentans per milliliter, 106-108 rhodopseudomonas palustris per milliliter, 104-106 geotrichum suaveolens per milliliter, 106-108 aspergillus clavatus per milliliter, 106-108 rough spore aspergillus per milliliter and 106-109 streptomyces globisporus per milliliter. According to the invention, excrements in a poultry house and excrements for accumulative fermentation are treated through a spraying manner, so as to effectively reduce the release amount of malodorous gases, and provide a technical support for the effective treatment of malodor in the livestock and poultry farms and the environmental protection. Besides, the deodorant special for livestock and poultry farms can also be sprayed to the livestock and poultry body surfaces, to keep the livestock and poultry body surfaces clean and sanitary, and be conducive to the growth of livestock and poultry.

The invention provides a small peptide additive for porcine feed and a preparation method thereof; the small peptide additive for porcine feed is characterized in that the additive is prepared by mixing base materials, saccharomyces cerevisiae liquid, lactobacillus acidophilus liquid, bacillus subtilis liquid and water according to a weight ratio of 100:3-4:3-4:5-7:35-4, and performing solid fermentation; the base materials comprise 50-70 wt% of soybean meal, 4-5 wt% of rapeseed meal, 10-15 wt% of fish meal, 5-8 wt% of vinasse, 2-5 wt% of corn starch, 1-3 wt% of wheat bran, 1-3 wt% of molasses, 1-3 wt% of yellow mealworm, and 0.5-1 wt% of sodium carbonate. The small peptide additive for porcine feed of the invention can increase the synthesis speed of protein for pig body, promote food ingestion of pigs, increase appetite, improve immunity, promote the absorption of mineral elements by pig body, regulate various physiological actions of the body, reduce stress, and improve the nutritional status of pig body.

PCT No. PCT / EP97 / 03560 Sec. 371 Date May 21, 1999 Sec. 102(e) Date May 21, 1999 PCT Filed Jul. 5, 1997 PCT Pub. No. WO98 / 09535 PCT Pub. Date Mar. 12, 1998Use of lactic acid bacteria in the preparation of a cream intended to coat all or part of an ice cream. The ice cream is an expanded and chilled cream, coated over all or part of its external surface with a non-expanded coating containing 103 to 109 cfu / g of lactic acid bacteria. Preferably, the expanded cream includes more than 106 cfu / g of lactic acid bacteria, the ratio (e / g) between the number of lactic acid bacteria in the coating (e) and the number of lactic acid bacteria in the expanded cream (g) being greater than 1. The coating may include 1% to 70% of a milk fermented by lactic acid bacteria, 0.5% to 5% of animal or vegetable proteins, a lactic fat content of from 2% to 20% and preferably the strain Lactobacillus acidophilus CNCM I-1225.

The invention discloses a mycotoxin degrading adsorbent, which contains bacillus subtilis, lactobacillus plantarum, lactobacillus acidophilus, leuconostoc lactis, lactobacillus delbrueckii, lactobacillus fermenti, lactobacillus salivarius, vitamin C, vitamin E, radix astragali, zinc methionine, potassium chloride, cassia bark, clove, oriental wormwood, folium artemisiae argyi, sweet wormwood, wild chrysanthemum flower, semen plantaginis, yeast cell wall and phytase. The mycotoxin degrading adsorbent disclosed by the invention has multiple effects including generation inhabitation, degradation and adsorption on various common mycotoxins, and can additionally boost immunities of pigs and promote excretion of toxic substances; the mycotoxin degrading adsorbent has excellent comprehensive performance and plays a remarkable role on productivities of piglets, and the mycotoxin degrading adsorbent can reduce disease rate, improve daily gain, increase feed digestibility and reduce feed conversion ratio. Moreover, the mycotoxin degrading adsorbent is low in dosage but effective and free from adsorption on drugs and other nutrient substances, and the adsorbent can be biologically degraded after being secreted and avoid pollutants; and the mycotoxin degrading adsorbent has good application value in breeding industry.