1212 results about "Lactobacillus acidophilus" patented technology

A preparation method of a ferment Chinese Traditional Herbs for feeding belongs to the production field of the feed additives. The technical proposal provided by the present invention is that one or various intestinal probiotics is inoculated on a cultivation matrix containing extracts of Chinese Traditional Herbs, and undergoes ferment cultivation in a proper condition. The probiotics include Lactobacillus acidophilus, Bacillus subtilis, and candida utilis, etc, and the selected Chinese Traditional Herbs include astragalus, atractlylis lancea formalyrata, codonopsis pilosula, pericarpium citri reticulatae, and atractylis atractylis ovata, etc. The ferment Chinese Traditional Herbs for feeding provided by the present invention used to breed the livestock and the poultry is capable of preventing and treating epidemic diseases and improving the growth of the animal. The biological ferment of the water extract of Chinese Traditional Herbs by the probiotics is utilized to produce the elements with higher activities, thereby improving the absorption, transportation, metabolism, activation and medical efficacy of the Chinese Traditional Herbs in human body.

The invention relates to micro-ecological Chinese medicine preparation for livestock and poultry by adoption of multi-strain fermentation production and a fermentation method. The compositions of traditional Chinese medicines in a culture medium of the fermentation preparation include: radix astragali, radix codonopsitis, Atractylodes macrocephala, Poria cocos, liquorice, medicated leaven, hawkthorn, angelica, Chinese rhubarb, Scutellaria baicalensis, radix isatidis, cordate houttuynia, sicklesenna seeds, Schisandra chinensis, Gynostemma pentaphylla, phellodendron, dried orange peel, radix bupleuri, curcuma, honeysuckle, Chinese gall, purslane and Quisqualis indica. The fermentation method is as follows: a multi-strain solid state fermentation strain is adopted for fermentation; bacillus subtilis, bacillus natto, bacillus licheniformis, beer yeast, Candida wtilis, Aspergillus niger, lactobacillus plantarum, Lactobacillus acidophilus and bifidobacteria are purified and subjected to anaerobic fermentation under the conditions of a rotating speed of 180 revolutions per minute at a temperature is 32 DEG C for 48 to 72 hours till the pH value reaches 4.0. The micro-ecological Chinese medicine preparation has the advantages that the micro-ecological Chinese medicine preparation uses probiotics to ferment different Chinese medicine compositions, and is micro-ecological Chinese medicine preparation which completely replaces antibiotics and has the advantages of no residual medicine, disease prevention, growth promotion and low cost.

The invention discloses a micro-ecological fruit-vegetable enzyme preparation method. The method comprises the steps of culturing 4 to 6 of six probiotics including lactobacillus acidophilus, bacillus coagulans, bacillus natto, saccharomyces cerevisiae, enterococcus faecium and bifidobacterium in a culture medium comprising ingredients such as brown sugar, liquorice and fresh sweet potato, and then culturing the probiotics through a diluted culture liquid containing brown sugar and liquorice in an activating manner to prepare a micro-ecological preparation, wherein the prepared micro-ecological preparation is a fermenting agent which is used for fermenting fruit pulp of fresh fruit and vegetable and traditional Chinese herbs. The microorganism enzyme in the ecological preparation and the natural vegetable enzyme in fruits, vegetables and Chinese herbs are compounded into a composite enzyme supplement by utilizing a micro-ecological fermentation technology, i.e. the micro-ecological fruit-vegetable enzyme. The micro-ecological fruit-vegetable enzyme can be made into a liquid type or a solid type (powder). An ideal effect is achieved in the fields such as medicine, healthcare and beauty.

The invention relates to a composite probiotics feed additive containing lactic acid bacteria. The composite probiotics feed additive is prepared by spraying, freezing, drying and compounding 40-60% of lactobacillus acidophilus, 15-20% of lactobacillus amylophilus, 5-10% of lactobacillus salivarius, 5-10% of streptococcus thermophilus, 5-10% of bacteroides thetaiotaomicron and 5-10% of glucose. All strains are separated from pig intestines chime. With the consideration of each part of an intestinal canal of animal, the lactic acid bacteria which produce lactic acid of the front section of the intestinal canal are combined with the bacteroides thetaiotaomicron which dissolve cellulose and polysaccharide of the rear section of the intestinal canal, the proportion among the bacteria is similar to the composition of corresponding bacteria in the intestinal canal of a healthy animal, so that the respective advantages can be developed well, the health of the animal is improved further, the feed digestibility is improved, and the production performance of the animal is improved.

A dual-effect microbial feed additive for ruminant is prepared through pulverizing corn, steaming, liquefying, saccharified, inoculating lactobacillus acidophilus and saccharomyce cerevisiae, fermenting to obtain living bacterium preparation, culturing bacillus subtilis, aspergillus oryzae and aspergillus niger in the culture medium prepared from corn, bran and soybean dregs, fermenting to obtain composite enzyme preparation, and proportionally mixing said living bacterium preparation with said composite enzyme preparation. It can promote the growth and health of ruminant and increase the utilization rate of feed.

The invention provides a preparation method for feed without antibiotics. Feed without antibiotics is complete pig feed without antibiotics, which is prepared from alkali protease and fermenting mixing feed of wholesome funguses and contains the wholesome funguses, a complex enzyme, complex organic acid and other microbe metabolites. The preparation method comprises the preparation steps: weighingthe raw materials of the fermenting feed: corn flour, bean dregs, fermenting bean dregs, fish meal and whey mist which are uniformly mixed; weighing immune polysaccharide, edible salt, calcium hydrophosphate, calcium carbonate, lysine, methionine, various vitamins and various minerals which are uniformly mixed; preparing wine making yeast, alkyl producing pseudofilament yeast, lactobacillus acidophilus, bacillus subtilis and bacillus licheniformis which are uniformly mixed; uniformly mixing all the raw materials and the funguses; adding water to the feed; bagging; sealing; fermenting until nogas is generated; discharging air; sealing again; sealing an outer packing bag and labeling to obtain a finished product. The feed without antibiotics prepared by the method has the advantages of good mouth feel, high nutritious value, easy assimilation and wide market prospect.

A vaginal treatment composition that employs a therapeutic agent to inhibit and/or treat vaginal infection is provided. The therapeutic agent is capable of inhibiting and/or killing Gardnerella (e.g., Gardnerella vaginalis), Candida (e.g., Candida albicans), and/or Trichomonas (e.g., Trichomonas vaginalis) pathogens. Desirably, such antimicrobial efficacy is achieved without substantially inhibiting the growth of Lactobacillus acidophilus. For instance, sugars and/or sugar alcohols may be employed in the present invention as a therapeutic agent for inhibiting and/or treating vaginal infection. In one particular embodiment, D-xylitol is used as the therapeutic agent.

The invention discloses a method for producing a complex micro-ecological preparation with microbial agents and enzyme. According to the action principles and characteristics of every microbial strain, the method adopts scientific combination and adds enzyme preparations and acidifier to enable microorganisms in the product to grow rapidly and take dominance in fermented feed and animal bodies so as to reduce the growth opportunity of miscellaneous bacteria. The preparation comprises the following components: bacillus subtilis, lactobacillus plantarum, candida utilis yeast, lactobacillus acidophilus, protease, amylase, cellulase, xylanase, mannanase, galactosidase, pectinase and citric acid. As all strains of the product adopt liquid deep fermentation culture, the non-polluting property of the product is guaranteed. Liquid microbial agents ensure that the number of bacterial colonies of the product is more than 100 million and the product is free from the pollution of other miscellaneous bacteria, while solid microbial agents ensure that the number of bacterial colonies of the product is more than 1 billion and the proportion of miscellaneous bacteria to effective bacteria is not more than 0.0001. The preparation contains bacillus, lactic acid bacteria and yeast, can apply to the processes of making green-yellow storage feed, fermenting feed, soaking feed, and the like, can be directly added to feed for feeding, and can improve the growth performance of ruminant animals, poultry, pigs, aquatic products and special animals.

The invention discloses a preparation method of freeze dried microbial powder of Lactobacillus acidophilus, which comprises the technical processes of fermented cultivation of Lactobacillus acidophilus, separation of a fermented fluid, emulsification of microbial soil and vacuum freeze drying of an emulsified solution. The preparation method is characterized in that the strain fermented cultivation comprises the steps as follows: a lactobacillus strain is inoculated into a fluid culture medium in a triangular flask, cultivated at the temperature of 30 to 40 DEG C for 10 to 30 hours, inoculated into a large fermentation flask and fermentation cultivated at the temperature of 35 to 40 DEG C for 10 to 30 hours, and sterilized at the temperature of 115 DEG C for 15 minutes; and fermentation and augmentation cultivation is carried out for 10 to 30 hours, the pH value of the fermented fluid is 5.5 to 6.5, and OD600 is over 2.0; the vacuum freeze drying of the emulsified solution comprises the steps: the emulsified solution is pre-frozen at the temperature of minus 60 to minus 40 DEG C for 1 to 5 hours, and then vacuum frozen and dried at the temperature of minus 60 DEG C and with the vacuum degree of 1 to 8 Pa for 10 to 30 hours. The freeze dried microbial powder of Lactobacillus acidophilus with the total plate count of 1.0 multiplied by 10 cfu/g can be preserved for 18 months at the temperature of minus 18 DEG C with unaffected liveness and stability.

The invention relates to a feeding addition agent for microorganism viable bacteria and a preparing method thereof. The addition agent consists of 1.5-2.5 parts of bacillus coagulans viable bacteria powder, 0.5-2.0 parts of lactobacillus acidophilus viable bacteria powder, 1.0-2.5 parts of lactobacillus plantarum viable bacteria powder, 0.5-2.5 parts of bacillus natto viable bacteria powder, 0.5-1.5 parts of candida utili viable bacteria powder and 2-4.5 parts of aspergillus niger viable bacteria powder. Each gram of the content of viable bacteria is as follows: bacillus coagulans is not less than 1.0*10cfu/g; lactobacillus acidophilus is not less than 1.0*10cfu/g; lactobacillus plantarum is not less than 1.0-10cfu/g; bacillus natto is not less than 1.0*10cfu/g; aspergillus niger is not less than 1.0*10cfu/g; and candida utili is not less than 1.0*10cfu/g. The invention has the advantages that the composition is reasonable, the preparation method is scientific, the usage scale is wide, the operation is simple and easy to master, and the cost is low. In addition, the invention can be used as the feeding stuff agent for various poultries, thus increasing the use ratio of the feeding stuff addition agent and strengthening the anti-disease ability of the poultries.

The invention relates to a method for preparing a highland barley-wheat germ deep processing product, in particular to a process for preparing highland barley-wheat germ biological fermented milk, which solves the problems that the highland barley-wheat germ deep processing product has a single form and that nutrients cannot be absorbed by a human body completely. Lactobacillus bulgaricus, streptococcus thermophilus and lactobacillus acidophilus are inoculated according to a certain ratio, and a beta-glucan functional factor, dietary fibers, trace elements, microorganisms and the like of highland barley, and the nutrients such as high-quality plant proteins, vitamin E and the like of the wheat germ are utilized. The method mainly comprises the steps of: the compounding of the highland barley and the wheat germ, grinding into thick liquid, enzymolysis, the addition of milk and lactic acid fermentation. The prepared highland barley-wheat germ lactobacillus beverage is rich in proteins, lactic acid bacteria, beta-glucan, various trace elements, vitamins and the like.

The invention belongs to the technical field of feed micro-ecology and relates to a composite micro-ecological preparation as well as a premixed material and an application of the preparation in a feed additive. The preparation contains Lactobacillus acidophilus powder and any two, three or four bacterium powders selected from Bacillus licheniformis, Bcillus pumilus, enteroccus and Bcillus subtilis; the preparation has high live bacteria content and low moisture content, has stress tolerance properties such as gastric acid resistance, cholate resistance, high temperature resistance, common antibiotics tolerance and the like and has the probiotic functions of producing acid and enzyme, inhibiting pathogenic bacteria and the like. By using the composite micro-ecological preparation of the invention, the utilization rate of the feed can be improved, the yields of meat, eggs, milk and the like are increased, the growth of animals is promoted, the immunity and disease-resistant capability of an animal organism are improved, and partial preparation can be used for replacing antibiotics, thereby changing the quality of an animal product.

A Lactobacillus plantarum BB9 capable of adhering to gastrointestinal tract and cholesterol removal is isolated from fruits and exhibits high BSH activity. In-vitro tests demonstrate that Lactobacillus plantarum BB9 has good acid and bile tolerance, and strong ability to adhere to intestinal cells. In-vivo tests show that hamsters fed high cholesterol diets added with BB9 strain have cholesterol and triglycerides in blood and liver effectively reduced, and their HDL-c/LDL-c ratios in blood are significantly higher than those of hamsters fed Lactobacillus acidophilus ATCC 43121 strain. It is hoped that the excellent acid and bile tolerance and intestinal adherence of the lactobacillus strain provided herein could produce cholesterol-lowering effect in humans.

The invention relates to a microbiological composite bacterial inoculum and a preparation method thereof, in particular to the microbiological composite bacterial inoculum used for preparing a biological bacterial fertilizer. The microbiological composite bacterial inoculum is prepared by culturing Rhodop seudanonas palustris, Candida utilis, Lactobacillus acidophilus, Bacillus subtilis and Streptomyces microflavus var. lactosus which are disclosed in a culture catalogue of the China Center for General Microbiology Culture Collection (CGMCC), wherein the serial number of the Rhodop seudanonas palustris is 1.2352; the serial number of the Candida utilis is 2.1180; the serial number of the Lactobacillus acidophilus is 1.1854; the serial number of the Bacillus subtilis is 1.1630; the serial number of the Streptomyces microflavus var. lactosus is 4.0891; and the weight ratio of the Rhodop seudanonas palustris to the Candida utilis to the Lactobacillus acidophilus to the Bacillus subtilis is 1:0.5:0.5:0.1. When used for preparing the biological bacterial fertilizer, the composite bacterial inoculum can effectively decompose and release crude fibers and other nutrient components in straws and livestock manure; and the prepared biological organic fertilizer can condition the soil, increase the activity rate of microorganisms in the soil, avoid soil hardening, improve the permeability of the soil, play a role in production increasing, and effectively solve the problem of the area source pollution of agricultural wastes in rural areas.

The invention provides a method for preparing edible bacteria synbiotic microcapsules. The method comprises the following steps: firstly, zymotic fluids of four strains of probiotic bacteria, namely Lactobacillus acidophilus, lactobacillus plantarum, lactobacillus casei and lactobacillus rhamnosus are prepared, then supernatants are removed after the speed of 5000r/min, and bacterial sludges are embedded for standby. 10g of each bacterial sludge of the probiotic bacteria to be embedded and 4g of edible bacteria Agaricus blazei powder with the fineness of 200 meshes are mixed with 40g of mixed colloid containing 2.5 percent of sodium alginate and 0.5 percent of flaxseed gum respectively; the first stirring is performed for 20min at a rotating speed of 200 r/min; the mixture is poured into a triangular flask which contains 1.5 times of volume of edible oil; the second stirring is performed for 20min at a rotating speed of 400r/min; a 1.5 percent CaCl2 solution is added into the mixture to be calcified for 3h; sterilized physiological saline is used to wash gel beads for three times; the gel beads are placed into a 0.6 percent chitosan solution with a pH value of 5.5 to perform a film-forming reaction for 15min, taken out, washed by the sterilized physiological saline and then placed into a citric acid solution with the concentration of 55mM to be liquefied for 30min; and wet microcapsules are obtained after the washing of the sterilized physiological saline, collected by centrifugating for 10min at a rotating speed of 150r/min, precooled, and finally freeze-dried to obtain the microcapsules.

The invention belongs to the technical field of food with homology of medicine and food, and particularly relates to a preparation method of rose ferment. The method comprises the steps of separating petals, sieving, protecting color, carrying out superfine grinding, carrying out fermentation pretreatment, planting bacteria for the first time, planting bacteria for the second time, planting bacteria for the third time, finely filtering and feeding collagen to obtain rose ferment liquid. According to the method, probiotic strains which are good for the intestinal tract of a human body are selected for use, and the fermentation temperature is strictly controlled, so that the loss of effective components in roses can be effectively reduced, and the activity of the rose ferment is maintained to the utmost extent; the dominant bacteria are implanted for three times, so that the growth of other bacteria can be effectively inhibited, and the product quality is stable; lactobacillus acidophilus, lactobacillus thermophilus and lactobacillus plantarum are selected, have symbiosis performance and can be planted in the intestinal tract after being fermented in a mixed way; furthermore, the comprehensive activity of ferment products can be better improved. The prepared rose ferment has a certain prevention function for chronic diseases such as heart disease and liver disease, and has the effects of soothing the liver and activating the spleen. After being eaten for a long time, the rose ferment also has the functions of smoothing the skin, whitening the skin and beautifying the face.

The invention provides a small peptide additive for porcine feed and a preparation method thereof; the small peptide additive for porcine feed is characterized in that the additive is prepared by mixing base materials, saccharomyces cerevisiae liquid, lactobacillus acidophilus liquid, bacillus subtilis liquid and water according to a weight ratio of 100:3-4:3-4:5-7:35-4, and performing solid fermentation; the base materials comprise 50-70 wt% of soybean meal, 4-5 wt% of rapeseed meal, 10-15 wt% of fish meal, 5-8 wt% of vinasse, 2-5 wt% of corn starch, 1-3 wt% of wheat bran, 1-3 wt% of molasses, 1-3 wt% of yellow mealworm, and 0.5-1 wt% of sodium carbonate. The small peptide additive for porcine feed of the invention can increase the synthesis speed of protein for pig body, promote food ingestion of pigs, increase appetite, improve immunity, promote the absorption of mineral elements by pig body, regulate various physiological actions of the body, reduce stress, and improve the nutritional status of pig body.

PCT No. PCT/EP97/03560 Sec. 371 Date May 21, 1999 Sec. 102(e) Date May 21, 1999 PCT Filed Jul. 5, 1997 PCT Pub. No. WO98/09535 PCT Pub. Date Mar. 12, 1998Use of lactic acid bacteria in the preparation of a cream intended to coat all or part of an ice cream. The ice cream is an expanded and chilled cream, coated over all or part of its external surface with a non-expanded coating containing 103 to 109 cfu/g of lactic acid bacteria. Preferably, the expanded cream includes more than 106 cfu/g of lactic acid bacteria, the ratio (e/g) between the number of lactic acid bacteria in the coating (e) and the number of lactic acid bacteria in the expanded cream (g) being greater than 1. The coating may include 1% to 70% of a milk fermented by lactic acid bacteria, 0.5% to 5% of animal or vegetable proteins, a lactic fat content of from 2% to 20% and preferably the strain Lactobacillus acidophilus CNCM I-1225.

The invention discloses a mycotoxin degrading adsorbent, which contains bacillus subtilis, lactobacillus plantarum, lactobacillus acidophilus, leuconostoc lactis, lactobacillus delbrueckii, lactobacillus fermenti, lactobacillus salivarius, vitamin C, vitamin E, radix astragali, zinc methionine, potassium chloride, cassia bark, clove, oriental wormwood, folium artemisiae argyi, sweet wormwood, wild chrysanthemum flower, semen plantaginis, yeast cell wall and phytase. The mycotoxin degrading adsorbent disclosed by the invention has multiple effects including generation inhabitation, degradation and adsorption on various common mycotoxins, and can additionally boost immunities of pigs and promote excretion of toxic substances; the mycotoxin degrading adsorbent has excellent comprehensive performance and plays a remarkable role on productivities of piglets, and the mycotoxin degrading adsorbent can reduce disease rate, improve daily gain, increase feed digestibility and reduce feed conversion ratio. Moreover, the mycotoxin degrading adsorbent is low in dosage but effective and free from adsorption on drugs and other nutrient substances, and the adsorbent can be biologically degraded after being secreted and avoid pollutants; and the mycotoxin degrading adsorbent has good application value in breeding industry.

The invention belongs to the field of biotechnology, and relates to Lactobacillus acidophilus and application thereof and a feed additive thereof and a premix compound thereof. The Lactobacillus acidophilus of the invention has strong stress tolerance, and can tolerate simulated gastric fluid and simulated bile salt so as to guarantee that a great quantity of viable bacteria can smoothly enter the intestinal tract of an animal, and the viable bacteria play a probiotic role, so that the immunity of the animal is improved, and the breeding cost is reduced. The product obtained from the Lactobacillus acidophilus of the invention through culture, high-density fermentation and addition of protectant has a high content of viable Lactobacillus acidophilus, can be added as a biological feed additive in the drinking water or feed for breeding animals, and can be prepared into feed such as premix compound and so on, so as to reduce the addition of antibiotics, indeed improve the intestinal tract health and the immunity of animals, and increase the production performance of animals.

The invention discloses a functional bio-protein feed ferment and a fermented protein feed.The functional bio-protein feed ferment comprises the following components according to parts by weight: 1-15 parts of Lactobacillus acidophilus, 1-10 parts of Lactobacillus plantarum, 10-20 parts of Bacillus coagulans, 10-20 parts of Bacillus licheniformis, 5-20 parts of Bacillus subtilis, 5-20 parts of Saccharomyces cerevisiae and 1-10 parts of Aspergillus oryzae.The fermented protein feed is obtained by fermenting a plant protein feed.The bio-protein feed ferment has the advantages of high fermenting efficiency, degrading thoroughness of harmful substances, high fermenting speed, low contaminating organism count and convenience of use, the fermented protein feed is good in attracting effect, high in content of beneficial bacteria, active peptides and organic acids, capable of partially replacing high-grade animal protein feeds among feeds, such as fish meal and spray-dried plasma protein, and capable of improving animal immunity, improving breeding environment, promoting growth of animals under breeding and effectively reducing usage of antibiotics.

The invention discloses a method for purifying mariculture wastewater by utilizing a compound microbial inoculant. The method comprises the following steps: (1) screening the compound microbial inoculant which consists of photosynthetic bacteria, nitrobacteria, denitrifying bacteria, candida utilis, lactobacillus acidophilus and bacillus; (2) activating the compound microbial inoculant by utilizing a culture medium: directly adding the strain into the culture medium based on 1% of inoculation amount (w/v), and activating the strain by a shaker at the temperature of 37 DEG C with the speed of 150rpm (revolution per minute) for 24 hours so that the total content of microbes is 7*10<8>-9*10<8>CFUs (colony forming units)/mL; and (3) adding the activated compound inoculant into the treated aquaculture wastewater, wherein the compound inoculant accounts for 0.06%-0.12% of the total weight of the aquaculture wastewater. The method has the advantages of no toxicity and harm on aquaculture animals, simple process, fast organic matter degradation speed, obvious effect, no drug residues and no secondary pollution. Therefore, the method has economic feasibility and is applicable to popularization and application to coastal aquaculture industry.

The invention discloses a preparation method for a sludge aerobic composting composite inoculums. According to the method, the Bacillus subtilis (No. 10066), the Bacillus licheniformis (No. 10103), the Lactobacillus acidophilus (No. 6075), the Candida utilis (No. 31272), and the Phanerochaete chrysosporium (No. 40719) that are preserved in China Center of Industrial Culture Collection (CICC) are subjected to individual culture, and are then mixed in proportion and inoculated into a fermentation culture to perform expanded culture, thus obtaining the composite inoculum. At the initial stage of inoculating the composite inoculums to sludge aerobic composting, the time for the compost body to reach a high temperature period is advanced by 4-6 days, the compositing period is shortened by 11-14 days, the stack moisture content is decreased by 18%-25%, and the total nitrogen content of the compost body is increased by 15%-20%, thus improving the composting effect.

The invention relates to a method for preparing selenium-rich low-cholesterol egg and the relevant product, belonging to health-care functional egg product production field, which comprises following steps: employing lactobacillus acidophilus and candida as bacteria, the culture medium is 0.5-2% yeast concrete and 0.5-2% glucose, inoculating bacteria in sodium selenite containing culture medium, culturing for 30-40hours, getting culturing solution containing selenium-rich probiotics, adding culturing solution in basic corn bean meal type ration , feeding chicken with it, and getting selenium-rich low-cholesterol egg products. The selenium content in egg products is 0.358-0.508 mg/ kg (wet weight), cholesterol in egg yellow content is 11.37-12.17 mg/ g (wet weight). The egg product is characterized by abundant nutrition, selenium supplement, low-cholesterol content, health-care function and effect of prevention of cardiovascular disease.

The invention discloses a biological organic fertilizer for improving the stress resistance and the output of paddy rice and a preparation method thereof. The biological organic fertilizer comprises the following components: an organic mixture adsorbed with a composite microbe liquid preparation, an organic substance, a Bacillus subtilis inoculum, a Bacillus licheniformis inoculum, a Saccharomyces cerevisiae inoculum, a Rhodopseudomonas palustris inoculum, Lactobacillus plantarum and Lactobacillus acidophilus. The biological organic fertilizer of the invention is rich in beneficial microbe florae and major and trace elements needed by the growth of various crops, so the ecological florae and the trace elements of soil can be supplemented and adjusted, phosphor dissolving and potassium releasing capabilities of soil are enhanced, effective nutrients can be strongly decomposed, converted and synthesized, the fertilizer utilization rate is improved, physical and chemical properties of soil are improved, the granular structure formation is promoted, hardened and morbid soil is restored, the soil fertility is increased, various soil-borne diseases are inhibited, the growth and the fructification of the crops are promoted, and the stress resistance and the output of the crops are substantially improved.

The invention relates to fermented chenopodium quinoa willd milk, flavored fermented chenopodium quinoa willd milk, a chenopodium quinoa willd sour milk beverage, and a preparation method of products, and belongs to the technical field of food industry. Chenopodium quinoa willd is used as a raw material for pulping, the protein content is not less than 2.9g/100g, a yoghourt fermenting agent is inoculated, fermentation is performed until acidity reaches to 70DEG T or above so that the fermented chenopodium quinoa willd milk is obtained. The yoghourt fermenting agent comprises a mixed bacterium agent which is obtained by mixing lactobacillus bulgaricus with streptococcus thermophilus in the mass ratio of the lactobacillus bulgaricus to the streptococcus thermophilus being 1 to 1 and of which the inoculum concentration is 20-25g/T, a mixed bacterium agent in which the mass ratio of lactobacillus acidophilus to bifidobacteria and to lactobacillus casei is 1 to 1 to 1 and of which the inoculum concentration is 12-15g/T, and lactobacillus plantarum of which the preservation number is CGMCC No.11763 and of which the inoculum concentration is 60-80ml/T. The chenopodium quinoa willd is used as the raw material, composite enzyme hydrolysis and composite active probiotic fermentation are used, and active probiotic fermented chenopodium quinoa willd milk and series products are prepared, so that industry blank is filled.

Fructooligosaccharide (FOS)-related protein nucleic acid molecules and polypeptides and fragments and variants thereof are disclosed in the current invention. In addition, FOS-related fusion proteins, antigenic peptides, and anti-FOS-related antibodies are encompassed. The invention also provides recombinant expression vectors containing a nucleic acid molecule of the invention and host cells into which the expression vectors have been introduced. Methods for producing the polypeptides of the invention and methods for their use are further disclosed.

The invention discloses a lactic acid bacteria composition capable of regulating intestinal flora functions and preparation method and application thereof. The active ingredients of the lactic acid bacteria composition capable of regulating intestinal flora functions are prepared from the following raw materials by weight: 20-40 parts of Lactobacillus acidophilus, 40-60 parts of Bifidobacterium longum, 20-60 parts of Lactobacillus rhamnosus, 30-60 parts of Streptococcus thermophilus and 35-75 parts of fructo-oligosaccharide. According to the lactic acid bacteria composition capable of regulating intestinal flora functions, lactic acid bacteria in the composition can reach a certain magnitude, and the raw materials are in reasonable compatibility, and have a good function of regulating intestinal flora. The lactic acid bacteria composition has good stability in a long time, and is suitable for the diabetics to eat.

The invention discloses a novel vinasse biological feed and a production process thereof. The novel vinasse biological feed comprises a vinasse culture medium and strains, and is characterized in that a formula of the vinasse culture medium comprises the following components of: 50%-70% of fresh white spirit vinasse, 5%-15% of corn starch, 5%-15% of rapeseed dregs and 15-25% of water, wherein the percentages refer to the percentages of all the components accounting for the total weight of the vinasse culture medium, 12-18mg of ferrous sulfate, 10-15mg of zinc sulfate, 8-12mg of manganese sulfate, 12-18mg of copper sulfate and 1-3g of calcium hydrogen phosphate are added into every 80-120g of the vinasse culture medium, and the strains comprise bacillus subtilis, trichoderma viride, lactobacillus acidophilus and brettanomyces custersianus. The technological process comprises the following steps of: antagonism experiment, expanded cultivation for the strains, purity and activity inspection, preparation and sterilization for the vinasse culture medium, vinasse microbial solid fermentation and treatment for vinasse biological feed after 9-11 days. The invention has the advantages of short cycle and high protein content.

The invention discloses a fermented feed. The fermented feed is prepared by the following steps of: performing amplification culture on Lactobacillus acidophilus ACCC10637 and Bacillus subtilis ACCC10619 respectively to obtain amplification culture solution by taking soybean whey as a raw material of a liquid culture medium, and performing amplification culture on Candida utilis ACCC20060 to obtain an amplification culture medium by a semi-solid culture medium; and according to the weight of a semi-solid-state fermentation culture medium, adding 3.2 percent of amplification culture solution of the Lactobacillus acidophilus ACCC10637, 1.0 percent of amplification culture medium of the Candida utilis ACCC20060, and 0.1 percent of amplification culture solution of the Bacillus subtilis ACCC10619 into the semi-solid-state fermentation culture medium respectively, and sealing and fermenting for 36 to 72 hours at the temperature of between 28 and 32 DEG C. When 10 percent of fermented feed is added into a pig feed, the utilization rate of the feed can be improved by 7 to 8 percent, the morbidity is reduced by 8 to 10 percent, and the culture environment is remarkably improved.