255results about How to "High content of live bacteria" patented technology

The present invention relates to microbial fodder additive, and a kind of microbial fodder additive comprising beneficial microbes and their metabolites and its preparation process and use. The microbial fodder additive is the mixture of fermented liquid or dry powder obtained with Bacillus subtilis, Bacillus cereus, lactobacillus and Saccharomyices cerevisiae and through high density deep layer liquid fermentation. It may be prepared into different specific liquid or powder preparations. The microbial fodder additive has high live bacillus content, long product preserving period and less foreign bacteria contamination, and has the functions of raise fodder utilization, promote animal's growth, increase animal product yield and quality, etc.

The present invention discloses a fruit and vegetable probiotic tablet and a preparation method thereof. The fruit and vegetable probiotic tablet uses probiotic powders such as lactobacillus plantarum powder as the main raw material, and the preparation method scientifically mixes modified dietary fibers, fruit and vegetable powder, oligosaccharides, plant extracts, protein powder, tea leaf extracts and traditional Chinese medicine extracts and etc., thus improves the content of soluble celluloses which are of real significance for probiotic flora, enhances the physiological activity of celluloses, thereby increases the species of intestinal probiotic flora as well as significantly enhances the colonization ability and time of endogenous and exogenous probiotics in the human intestinal tracts, effectively inhibits the growth and reproduction of harmful intestinal bacteria, especially gram-negative bacteria, and fully regulates the composition of the intestinal probiotic flora. The prepared fruit and vegetable probiotic tablet has a high biological activity, a long human intestinal colonization time, and a significant weight loss effect, and is suitable for a wide range of people.

The invention discloses a lactobacillus leavening agent, a preparation method thereof and a special bacterial strain. The preservation serial number of lactobacillus is CCTCC M208151. An active constituent of the lactobacillus leavening agent is the lactobacillus. The invention also discloses a method for preparing the lactobacillus leavening agent, which comprises the following steps: firstly, leavening and culturing lactobacillus plantarum SC79 CCTCC M208151 in SC79 optimal culture medium; and secondly, collecting thallus in the step 1 and then adding a protective agent to the thallus to obtain the leavening agent. The lactobacillus leavening agent has small dosage, quick acid production speed through mixing the lactobacillus leavening agent and a harvestless exopolysacchatide yoghourt bacterial strain to prepare yoghourt, better relative viscosity, elasticity, denseness and adhesiveness than that of the conventional yogurt and small syneresis sensibility of formed sticky yogurt colloids, larger water holding capacity than that the conventional yogurt and hard whey separation.

The invention discloses complex microbial inoculant which comprises the following active ingredients: bacillus subtilis, bacillus megatherium, trichoderma koningii, aspergillus oryzae, trichoderma viride, geotrichum candidum, aspergillus niger, rhizopus oligosporus, lactobacillus plantarum CICC20768 and azotobacter chroococcum. Correspondingly, the invention further discloses a method for preparing an organic fertilizer from the complex microbial inoculant. The method comprises the following steps: a. uniformly mixing the complex microbial inoculant with a plant fiber raw material to obtain a fermented raw material; b, composting and fermenting the fermentation raw material; c, drying the composted fermentation raw material to obtain the organic fertilizer. microbes of the complex microbial inoculant have no antagonism, and can effectively cooperate in the production process of the organic fertilizer, and the chemical components of the production raw materials can be sufficiently utilized to finally produce the high-quality organic fertilizer. The produced organic fertilizer reaches the parameter indexes of the standard (NY884-2012) of China, can improve the planting property of soil, is beneficial to optimization of soil microbial flora, and can provide comprehensive nutrients to a plant root system.

The invention provides a microbial preparation for improving freshwater aquaculture water body. The microbial preparation comprises the following raw materials by weight percentage: 25-30 wt% of bacillus subtilis, 25-30 wt% of bacillus licheniformis and 15-20 wt% of rhodopseudomonas palustris. The invention also provides a method for preparing the microbial preparation, which comprises the following steps of: sequentially inoculating the seed culture liquids of the bacillus subtilis, the bacillus licheniformis and the rhodopseudomonas palustris into a culture medium, fermenting to obtain a bacteria liquid, and mixing the obtained bacteria liquid with a carrier and nutrient substances to obtain the microbial preparation. The microbial preparation provided by the invention can significantly improve the water quality of the aquaculture water body and can also improve the meat quality of bred animals.

The invention belongs to the field of biotechnology, and relates to Lactobacillus acidophilus and application thereof and a feed additive thereof and a premix compound thereof. The Lactobacillus acidophilus of the invention has strong stress tolerance, and can tolerate simulated gastric fluid and simulated bile salt so as to guarantee that a great quantity of viable bacteria can smoothly enter the intestinal tract of an animal, and the viable bacteria play a probiotic role, so that the immunity of the animal is improved, and the breeding cost is reduced. The product obtained from the Lactobacillus acidophilus of the invention through culture, high-density fermentation and addition of protectant has a high content of viable Lactobacillus acidophilus, can be added as a biological feed additive in the drinking water or feed for breeding animals, and can be prepared into feed such as premix compound and so on, so as to reduce the addition of antibiotics, indeed improve the intestinal tract health and the immunity of animals, and increase the production performance of animals.

The invention discloses a method for manufacturing a biological organic fertilizer, in particular to a method for producing a biological organic fertilizer by using dairy manure, straw and the like as raw materials. The biological organic fertilizer comprises the following components in percentage by weight: 20 to 50 percent of dairy manure, 20 to 50 percent of rice straw, 0 to 50 percent of garlic straw, 1 percent of gypsum powder and 1 to 2 percent of lime powder. The method comprises the following processes of: pre-treating the raw materials containing 20 to 50 percent of dairy manure, 20 to 50 percent of rice straw, 0 to 50 percent of garlic straw, 1 percent of gypsum powder and 1 to 2 percent of lime powder, performing primary fermentation and secondary fermentation, sowing, fruiting, harvesting, drying mushroom residues, adding a nutrition complex agent and packing.

The invention discloses weight-losing probiotic tablets and a preparation method thereof. According to the weight-losing probiotic tablets and the preparation method thereof, probiotic powder such as lactobacillus plantarum is taken as a main raw material, and modified dietary fibres, fruit and vegetable powder, oligosaccharides, plant extract, protein powder, tea leaf extract, Chinese herb extracts and the like are scientifically compounded, thus increasing the content of soluble celluloses with real significance in probiotic groups, and enhancing the physiological activity of the celluloses, and then remarkably improving the planting capacity and the planting time of endogenous and exogenous probiotics in human intestinal tracts, effectively suppressing the growth and the reproduction of intestinal harmful bacteria, in particular, Gram negative bacteria, and adequately regulating the composition of the intestinal probiotic groups while increasing the types of the intestinal probiotic groups; the prepared weight-losing probiotic tablets are high in bioactivity, long in planting time in the human intestinal tracts, remarkable in weight-losing effect, and wide in the range of appropriate crowds.

Belonging to the premix field, the invention relates to an additive premix for laying hens. The premix of the invention mainly contains: soybean meal, calcium hydrogen phosphate, stone powder, salt, compound vitamin, nicotinic acid, D-calcium pantothenate, biotin, choline chloride, copper sulfate, ferrous sulfate, zinc sulfate, manganese sulfate, potassium iodide premix, sodium selenite premix, ethoxy quinoline, phytase, microecologics, xylo oligosaccharide, bentonite and rice husk. With xylo oligosaccharide and a plurality of probiotics and without antibiotic, the premix in the invention hasa reasonable formula and high viable bacteria content. Animal feeding experiments prove that the additive premix in the invention has ideal feeding effects. The ammonia concentration, the death and culling rate, and the broken egg rate can be reduced, while the laying rate and egg weight are enhanced. The composite premix provided in the invention can well promote the laying performance and health status of laying hens, and meet the needs of mass raising households, and is suitable for green and healthy culture so as to produce pollution-free egg products.

The invention relates to a compound microecological preparation, in particular to the compound microecological preparation containing bacillus subtilis and lactobacillus plantarum as well as application of the compound microecological preparation in a daily ration of pigs, and belongs to the technical field of the microecological preparations. The compound microecological preparation comprises the bacillus subtilis CGMCC NO. 8148 bacterial powder and the lactobacillus plantarum CGMCC NO. 8150 bacterial powder. The compound microecological preparation can be applied to a piglet, a sow, a breeding boar and a finishing pig, can improve the utilization efficiency of a fodder and an intestinal micro-ecological system, promote growth of a pig, enhance the immunity and premonition of the pig, and replace antibiotics. Therefore, the quality of pork is improved.

The invention provides a compound microorganism preparation used for freshwater aquiculture water body improvement, which comprises the raw materials by weight percentage: 25-30wt% of bacillus subtilis, 25-30wt% of bacillus amyloliquefaciens and 15-20wt% of thiobacillus denitrificans. The invention further provides a preparation method of the microorganism preparation, which comprises the steps that bacillus subtilis, bacillus amyloliquefaciens and thiobacillus denitrificans seed culture fluids are sequentially inoculated into a culture medium and fermented; an obtained bacterium fluid is mixed with a carrier and a nutritional substance; and the microorganism preparation is obtained. The microorganism preparation can obviously improve the water quality of an aquiculture water body, and can improve the meat quality of an aquiculture animal.

The invention relates to a composite micro-ecological preparation and application thereof, in particular to a composite micro-ecological preparation containing multiple probiotics and application thereof. The preparation contains any three or four of bacillus licheniformis CGMCC No. 2383 powder, bacillus subtilis powder, enterococcus faecalis CGMCC No. 2386 powder, lactobacillus acidophilus powder and saccharomyces cerevisiae CGMCC No. 2388 powder. The preparation has high content of live bacteria, and has the adversity resistance such as gastric acid resistance, bile salt resistance, high-temperature resistance, common antibiotic resistance and the like and the probiotic functions of producing acid and enzyme and resisting pathogenic bacteria. The variety and the proportion of the probiotics and carriers can be determined according to different animals and different animal growth phases. The micro-ecological preparation can improve the feed utilization efficiency, increase the yield of meat, eggs and milk, promote the growth of animals, improve the immunity and the disease resistance of the animals, replace the antibiotic and improve the quality of animal products.

The invention discloses a probiotic foodstuff and a preparation method thereof. In the fermentation process of yoghourt, a functional starter, germinated brown rice, Chinese herbal medicine extract with anti-heat-and-cold irritability and a stabilizing agent capable of effectively enhancing stability are added; temperature-varying yoghurt fermentation enables propagation of lactic acid bacteria to be maximized and anti-heat-and-cold stress capability of lactic acid bacteria to be improved; non-thermal processing technology is employed for pretreatment of yoghurt and fruit and vegetable pulp so as to prevent infection with infectious microbes in preparation process, improve juice yield of the fruit and vegetable pulp, increase the content of soluble cellulose in the fruit and vegetable pulp and prevent browning of the fruit and vegetable pulp; and a scientifically compounded cryoprotectant with good anti-refrigeration effect is added during mixing of the fruit and vegetable pulp and the yoghurt. Eventually, the prepared probiotic foodstuff has fine mouthfeel, uniform texture, stable state, natural quality, a great number of live bacteria, strong functionality and long shelf life, wherein the content of the live bacteria is 1.75 * 10<11> to 2.55 * 10<11> CFU/g, and the shelf life is 30 to 36 months at normal temperature.

The invention belongs to preparation for microorganism-decomposing agents, and in particular discloses a method for preparing a microorganism-decomposing agent used for producing a biological organic fertilizer by livestock and poultry manure fermentation. The method comprises the following steps of: respectively inoculating Saccharomyces cerevisiae AS1.2414 and Saccharomyces cerevisiae AS1.1854 into a culture medium which contains a carbon source, a nitrogen source and nutrient substances for fermentation; inoculating Rhodopseudomonas palustris AS 1.2349 into a culture medium which contains nutrient substances for fermentation; and mixing saccharomyces cerevisiae fermentation liquor, rhodopseudomonas palustris fermentation liquor and rhodopseudomonas palustris fermentation liquor according to a volume ratio of 50:25:25, and taking a mixture of rice bran and bran as a carrier to adsorb the mixed fermentation liquor so as to prepare a solid decomposing agent. The method solves the problems of the quality and the speed of composting influenced by the relatively low composting speed in the composting process of the prior art, and has the advantages of effectively reducing the composting time, improving the compost maturity and the production efficiency, and the like.

The present invention discloses a fermented feed-containing 5% laying hen premix and a laying hen full price daily diet prepared from the premix. The fermented feed consists of corn, tenebrio molitor meal, soybean meal, cottonseed meal, apple residues, chili meal, corn soaking liquid sprayed corn husk, wheat bran, microorganism liquid and water. The preparation steps of the fermented feed are as follows: raw material crushing and weighing, raw material primary mixing, strain activating, strain and raw material secondary mixing, solid anaerobic fermenting, and wet basic state fermentation product drying. The fermented feed-containing 5% laying hen premix comprises dry basic state fermentation feed and other materials. The daily diet consists of corn, soybean meal, stone powder, and the fermented feed-containing 5% laying hen premix. The advantages of the daily diet are that after the laying hens are fed by the daily diet, the laying hen egg production rate and other egg laying performances and egg product quality are significantly improved, egg production peak period is extended, immune function is enhanced, anti-stress ability is improved, and the daily diet can superimposedly play the roles of regulating the microecology of beneficial bacterium flora and regulating neurotransmitter in the central nervous system.

The invention discloses freeze-dried tablets and a preparation method thereof. According to preparation method of the freeze-dried tablets, a functional leavening agent, functional germinated brown rice, Chinese herbal medicine extracts with ambient temperature stress resistance, and a stabilizer capable of effectively improving stability are added in the yoghurt fermentation process; through the adoption of a variable-temperature yoghurt fermentation technology, the propagation of lactic acid bacteria is maximized, and the ambient temperature stress resistance of lactic acid bacteria is improved; yoghurt and fruit puree are pre-processed by adopting a non-thermal processing technology, so that the sundry bacteria contamination is prevented during the process, the juice yield of fruit juice is increased, the content of soluble cellulose of the fruit puree is increased, and the browning of the fruit puree is prevented; in addition, a freezing protecting agent scientifically compounded and having better anti-freezing effect is added during the mixing of the yoghurt and fruit puree; finally, the freeze-dried tablets which are delicate in mouth feel, uniform in texture, stable in state, great in quantity of live bacteria, good in functionality and long in shelf life. The content of live bacteria in the freeze-dried tablets is 1.55*10<11>-2.52*10<11> CFU/g, and the shelf life is 30-36 months at the normal temperature.

The invention provides a preparation method for composite bacillus microcapsule preparations. The method comprises the steps that bacillus subtilis YB-1 CGMCC No.5116 bacterial suspension, bacillus cereus YB-2 CGMCC No.5117 bacterial suspension and bacillus flexus XH-1 CGMCC No.5115 bacterial suspension are mixed and fermented to obtain fermentation products; protective agents and bacteria carrier fluid are added into the fermentation products, the mixture is fully shaken and sprayed in CaCl2 curing liquid to conduct curing, after curing is conducted, filtering, low temperature freeze drying, adsorption carrier absorbing and drying are conducted in sequence, and the composite bacillus microcapsule preparations are obtained. The composite bacillus microcapsule preparations have the advantages that the microbial content is high, intimidation of high temperature, oxygen, acid environment on composite bacilli can be effectively avoided, and the application effect of the composite bacilli in the aquatic animal breeding process is greatly improved.

The invention belongs to the technical field of microorganism bacterium agent fermentation, and particularly relates to a high-density solid fermentation method for agricultural bacillus. The fermentation method comprises the following steps that 1, sterilization treatment is performed on fermentation materials; 2, a fermentation device is sterilized; 3, the fermentation materials in the step 1 are put into the fermentation device in the step 2, and bacillus is inoculated into the fermentation materials; 4, aerated fermentation is performed on the fermentation materials; 5, the fermentation materials are dried. According to the method, the raw materials of a culture medium are simple, choosing range is wide, and the cost is low; the floor space is small, the requirement on equipment is low, special machines and tools are not needed, assembly is convenient, energy consumption is low, and therefore the production cost is much lower than that of liquid fermentation and only accounts for 30%-50% of that of liquid fermentation; compared with pile fermentation and traditional shallow tray fermentation, the fermentation method has the advantages that the requirements for temperature, humidity and ventilatory capacity in the bacillus growth process are taken into account, and the problems such as metabolic heat removal and mass transfer in the bacillus solid fermentation process are effectively solved.

The present invention discloses a blueberry tablet candy with beautifying and antibacterial efficacy and a preparation method thereof, and belongs to the technical field of deep processing of blueberry. The method is as below: processing a main raw material blueberry into a blueberry syrup by using a non-thermal for processing method; uniformly mixing the blueberry syrup with milk, sugar, a herb extract, honey and a stabilizer; adding a leavening agent; fermenting by a segmented variable temperature fermentation method to prepare a blueberry yogurt, so as to maximize proliferation of probiotics and gain good thermal stress ability of resistance to coldness and hotness; then adding a protecting agent with good anti-freezing effect and freeze-drying into a powder, so as to obtain a functional blueberry yogurt freeze-dried powder with high content of viable probiotics and strong resistance to coldness and hotness; finally compounding the blueberry yogurt freeze-dried powder with an alfalfa extract, germinated brown rice, a plant extract, dextrin, probiotic factor and magnesium stearate scientifically; and tabletting by a multi-impact rotary tabletting machine to obtain the blueberry tablet candy with high probiotic live bacteria content, good stability, long shelf life and the efficacies of improving the body's immunity, delaying aging, beautifying and resisting bacteria.

The invention discloses compounded bee pollen and a preparation method thereof. Bee pollen is adopted as a main raw material, natural plant source enzymes with high enzyme activity and rich enzyme systems and pupa intestinal tract extract including queen bee larva and drone pupa saliva with the most original bee pollen forming process and the most complete enzyme systems and intestinal tract enzyme systems are scientifically compounded, the microwave high-temperature instant puffing technology and the probiotic fermentation technology are adopted, fast, effective and deep wall breaking is carried out on the bee pollen through special biological enzymolysis, microwave high-temperature instant low-temperature puffing and probiotic fermentation, wall-broken bee pollen is prepared and then is scientifically compounded with one or more functional auxiliary materials, the healthcare bee pollen with the high wall breaking rate, high efficiency, low cost and high functionality is prepared, and the natural flavor and nutritional ingredients of the bee pollen are kept to the maximum extent.

The invention discloses probiotic dry red wine and a preparation method thereof. The preparation method includes that dry red wine and rose flowers are used as raw materials, and low-temperature extraction technologies like high-voltage pulse electric field, microwave, ultrasound and biological enzymolysis are organically combined, so that functional substances and fragrant ingredients of the rose flowers can be retained to maximum extent; probiotics like functional Lactobacillus plantarum are used as fermentation agents, and temperature-variable fermentation and graded inoculation are adopted, probiotic multiplication and accumulation of functional metabolite can be realized to maximum extent, malic acid in the dry red wine can be further converted into lactic acid, acor of the dry red wine can be eliminated, and taste, healthcare function and wine stability of the dry red wine can be improved; the high-voltage pulse electric field, ultrasonic technology and peracetic acid are combined to accelerate aging, so that the probiotic dry red wine obtained has quality equivalent to that of existing fermented common dry red wine after aging for 8-10 years, and is good in aging effect, short in period and high in efficiency.