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Compound microecological preparation and additive, as well as premix thereof

A compound microecology and preparation technology, applied in the direction of microorganisms, microorganism-based methods, animal feed, etc., can solve problems such as the instability of gastric acid and bile salts, the number of viable bacteria, and the difficulty in meeting treatment needs, etc., to improve intestinal Effects of improving road micro-ecological flora, improving immunity and disease resistance, and improving feed utilization efficiency

Inactive Publication Date: 2014-08-06
漳州大北农农牧科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are many types of micro-ecological products on the market, and the quality is uneven. There are mainly the following problems: (1) The content of live bacteria is low, and studies have shown that if the concentration of a bacteria in the cecal content is less than 10 7 cfu / g, the enzymes and metabolites produced by the bacteria are not enough to affect the host, and it is difficult to meet the needs of treatment; (2) the water content is too high, which greatly shortens the shelf life of the probiotics, which also affects the quality of the probiotics. (3) Not resistant to antibiotics; (4) Unstable to gastric acid and bile salts, so that only a few strains are still active after entering the intestinal tract, and the number of viable bacteria required to play a role cannot be reached
Due to these quality problems of probiotics, it affects its application effect in animal feeding and limits the wide application of probiotics

Method used

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  • Compound microecological preparation and additive, as well as premix thereof
  • Compound microecological preparation and additive, as well as premix thereof
  • Compound microecological preparation and additive, as well as premix thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Isolation, screening, breeding and identification of embodiment 1 fine strains

[0028] 1) Bacterial enrichment culture: inoculate fresh intestinal content samples of healthy pigs into LB liquid medium, and culture and proliferate at a constant temperature of 37°C for 48 hours;

[0029] 2) Isolation and purification of Bacillus subtilis: For step 1) the enrichment culture was bathed in water at 80°C for 20 minutes, then the treatment solution was taken and diluted in multiples, spread on LB medium and cultured at 37°C for 24 hours, and each of them with characteristic morphology was picked Bacterial colonies were cultured and isolated by streaking until they were pure colonies, checked under a Gram staining microscope, and the isolated suspected pure colonies were inoculated on the slant of the test tube to proliferate, and then stored in a refrigerator at 4°C until use;

[0030] Colony morphology: the colony is round, with a smooth surface, milky white, 1-3mm in size; ...

Embodiment 22

[0035] Stress resistance and biological performance measurement of embodiment 2 2 excellent bacterial strains

[0036] Determination of biological properties and stress resistance of 2 strains: Bacillus subtilis CGMCC No.8148 and Lactobacillus plantarum CGMCC No.8150:

[0037]1) Preparation of cultures of Bacillus subtilis CGMCC No.8148 and Bacillus licheniformis CGMCC No.2383: inoculate the slant strains stored in the refrigerator into LB seed medium for activation, and cultivate them at 35°C and 100rpm for 16 hours to obtain the product;

[0038] 2) Preparation of Lactobacillus plantarum CGMCC No.8150 culture: inoculate the slant bacteria stored in the refrigerator into MRS seed medium for activation, and cultivate at 30°C and 150rpm for 16 hours to obtain the product;

[0039] 3) Determination of acid resistance: Inoculate the cultures prepared above at 10% inoculum volume into artificial simulated gastric juice with pH values ​​of 2.0, 3.0, and 4.0, readjust the pH values ...

Embodiment 3

[0074] The preparation of embodiment 3 Lactobacillus plantarum bacteria powder

[0075] 1) Plate culture rejuvenation: Inoculate the Lactobacillus plantarum strains on the MRS plate medium, culture at 37°C for 16 hours, rejuvenate the Lactobacillus plantarum bacteria and form a single colony, pick a single colony on the inoculation medium, and incubate at 37°C Cultivate for 14h;

[0076] 2) Preparation of primary seeds: transfer the Lactobacillus plantarum cultured in step 1) into a 2L shaker flask filled with 300ml of MRS liquid medium, and culture at 37°C for 18 hours until the late logarithmic period to obtain primary seeds;

[0077] 3) Preparation of secondary seeds: Transfer the primary seeds prepared in step 2) to a 100L seed tank filled with 75LMRS seed medium, cultivate at 37°C and 80rpm for 18 hours to obtain secondary seed liquid.

[0078] 4) Preparation of Lactobacillus plantarum fermentation liquid: inoculate the secondary seed liquid prepared in step 3) to 0.75 m...

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Abstract

The invention relates to a compound microecological preparation, in particular to the compound microecological preparation containing bacillus subtilis and lactobacillus plantarum as well as application of the compound microecological preparation in a daily ration of pigs, and belongs to the technical field of the microecological preparations. The compound microecological preparation comprises the bacillus subtilis CGMCC NO. 8148 bacterial powder and the lactobacillus plantarum CGMCC NO. 8150 bacterial powder. The compound microecological preparation can be applied to a piglet, a sow, a breeding boar and a finishing pig, can improve the utilization efficiency of a fodder and an intestinal micro-ecological system, promote growth of a pig, enhance the immunity and premonition of the pig, and replace antibiotics. Therefore, the quality of pork is improved.

Description

technical field [0001] The invention relates to a compound micro-ecological preparation, mainly relates to the compound micro-ecological preparation containing Bacillus subtilis and plantaractobacillus and its application in pig rations, and belongs to the technical field of micro-ecological preparations. Background technique [0002] At present, antibiotics are still used in the prevention and treatment of animal diseases, but due to the long-term and large-scale use of antibiotics, various disadvantages such as increased drug resistance of pathogenic bacteria, imbalance of flora, decreased immunity, high disease recurrence rate, and drug residues are becoming more and more serious. Serious, but also seriously endanger the animal body and human health. In this situation, it is an inevitable trend to change the concept of breeding, put prevention before treatment, and develop and apply new green feed additives that are non-toxic and drug-free. [0003] Microecological prepa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/16C12R1/125C12R1/25A23K10/18A23K10/37A23K20/163A23K20/20A23K50/30
CPCY02P60/87
Inventor 陆黎明张立彬黄梅丹戴佳佳温灿权谢燕婷贠桂玲韩科厅
Owner 漳州大北农农牧科技有限公司
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