1014 results about "Lactobacillus" patented technology

The invention relates to the technical field of microorganisms, and in particular relates to application of a strain of lactobacillus mucus TG018. The invention further relates to a preparation method of the lactobacillus mucus TG018. The preservation number of the lactobacillus mucus TG018 is CCTCC (China Center for Type Culture Collection) No: M 2023514; the lactobacillus mucus TG018 can be used for producing nicotinic acid, serving as an antioxidant and / or preparing the antioxidant, or can be applied to medicines for reducing uric acid.

The invention relates to the field of microorganisms, in particular to a plant lactobacillus and application thereof in bacteriostasis. Specifically, the preservation number of the plant lactobacillus LP06 is CGMCC (China General Microbiological Culture Collection Center) No. 28412. The plant lactobacillus LP06 provided by the invention can be used for inhibiting the growth of debaryomyces hansenii, candida tropicalis and penicillium rochei, and also has an inhibiting effect on super bacteria acinetobacter baumannii, pseudomonas aeruginosa, klebsiella, escherichia coli, staphylococcus aureus and listeria monocytogenes. The plant lactobacillus LP06 provided by the invention has broad-spectrum bacteriostatic ability, and bacteriostatic powder prepared from the bacterium can be used as a biological preservative for corrosion prevention of dairy products such as yoghourt, cheese and the like and other foods.

The invention discloses a probiotic with the depression improving effect and a microecological preparation and application thereof, and is characterized in that the probiotic comprises lactobacillus mucus PC17 with the preservation number of CGMCC No.33866, the active ingredients of the lactobacillus microecological preparation comprise thalli of the lactobacillus mucus PC17, and a preparation method of the microecological preparation comprises the following steps: adding the lactobacillus mucus PC17 into the lactobacillus mucus PC17, uniformly mixing the lactobacillus mucus PC17, the lactobacillus mucus PC17, the lactobacillus mucus PC17, the lactobacillus mucus PC17, the lactobacillus mucus PC17, the lactobacillus mucus PC17 and the lactobacillus mucus PC17 The lactic acid bacteria are inoculated into a fermentation culture medium according to a volume ratio of 1-5% to prepare the lactic acid bacteria microecological preparation, can be used for preparing antidepressant drugs, and has the advantages of being capable of promoting RIN14B cells to secrete 5-hydroxytryptamine and 5-hydroxytryptophan, reducing the levels of interleukin-1beta, interleukin-6, tumor necrosis factor-alpha, corticosterone and corticotropin in serum, and improving the antidepressant activity of the antidepressant drugs. The levels of aminobutyric acid and brain-derived neurotrophic factors in serum are improved, and the depression-like behaviors of CUMS depression model mice are obviously improved.

The invention relates to a processing method of fermented edible sheepskin based on plant lactobacillus, and belongs to the technical field of food processing. Aiming at the problems of fiber damage, high microbial pollution risk, texture hardening after marinating and the like caused by chemical treatment in the traditional sheepskin processing, the invention provides a solution of combining biological fermentation with gradient temperature control. The method is characterized by comprising the following steps: soaking sheepskin (subjected to unhairing treatment) in a 4-6% sodium carbonate solution (40 DEG C, 2 hours) for pretreatment; activating the plant lactobacillus until the viable bacterium concentration is 8 * 8 * 10 < 8 >-1.2 * 10 < 9 > CFU / mL; performing staged fermentation on the sheepskin and the bacterial suspension according to a ratio of 1: 3 at 30-35 DEG C for 18 hours, and supplementing a 0.1% glucose solution of less than or equal to 5% during the fermentation; washing with distilled water and draining; the marinating liquid contains 8% of table salt, 2% of anise, 1% of cinnamon and 0.5% of clove. The method is used for producing edible sheepskin products with flexible texture and up-to-standard microbiological indexes.

The invention discloses a ginseng extract rich in rare saponins and preparation and anti-aging application thereof, and belongs to the technical field of oral beauty and skin care products, the ginseng extract is obtained by fermentation treatment of ginseng powder through phytobacterium plantarum, and the preservation number of the phytobacterium plantarum is GDMCC No.64374. The ginseng extract rich in rare saponin is fermented by specific plant lactobacillus, ginsenoside is efficiently converted into CK and F1, various natural plant components such as ginseng polysaccharide are reserved, and a specific synergistic effect is generated. Through verification, the prepared ginseng extract is high in bioavailability, can more effectively inhibit cell inflammation, and can more effectively recover the mitochondrial function of senescent cells and inhibit cell senescence. The preparation method provided by the invention is high in operability and easy to standardize and intellectualize. Compared with a traditional extraction and purification method, the adopted fermentation method is more environmentally friendly and efficient, and large-scale production can be achieved in industrial production.

The invention provides a plant lactobacillus TH13, and the preservation number of the plant lactobacillus TH13 is CGMCC (China General Microbiological Culture Collection Center) NO.32112. The plant lactobacillus TH13 provided by the invention can be used for fermenting folium cortex eucommiae and enzymatically hydrolyzed bone meal, and the fermentation product of the plant lactobacillus TH13 has high content of chlorogenic acid, proline, glycine and lysine. The fermentation product can significantly improve the content of alkaline phosphatase (ALP) and osteocalcin (OCN) in mouse serum and reduce the content of tartrate-resistant acid phosphatase (TRACP). Therefore, the fermentation product can significantly improve the bone mineral density of the femur, increase the number of bone trabecula and significantly reduce the separation degree of the bone trabecula, thereby promoting bone formation of a mouse with senile osteoporosis, inhibiting bone resorption and improving senile bone loss and bone microstructure deterioration. The preparation method of the probiotic preparation containing the plant lactobacillus and the probiotic fermentation product is simple, and large-scale production can be realized.

The invention discloses a plant lactobacillus. The plant lactobacillus has the characteristic of efficiently tolerating 30% (w / v) cistanche substrate. The invention also discloses a medicinal and edible composition consisting of 85 parts of cistanche deserticola, 5 parts of rehmannia, 5 parts of fructus alpiniae oxyphyllae and 5 parts of Chinese yam, the composition is fermented by using plant lactobacillus, and the cistanche deserticola fermentation liquor is prepared by adopting a multi-stage temperature fermentation process to ferment for 16 hours. A non-targeted metabonomics result shows that the phytobacterium plantarum activates an upstream channel for synthesis of phenylethanoid glycoside substances, and the abundance of forsythiaside B is increased by 133 times compared with that before fermentation. The fermentation liquor can achieve the functions of reproductive anti-aging, kidney protection and body endurance improvement by down-regulating COX-2a and TGF-beta-1 gene expression.

The invention discloses a composition with high ultraviolet damage resistance as well as a preparation method and application thereof, and particularly belongs to the technical field of skin care product raw materials. The composition with high ultraviolet damage resistance is prepared by performing solid-state fermentation on radix scutellariae roots, radix ligustici sinensis and radix puerariae through compound bacteria consisting of bacillus subtilis, lactobacillus bulgaricus and bacillus coagulans and then performing solvent extraction. The composition with high ultraviolet damage resistance has excellent effects of resisting oxidative damage and inhibiting inflammatory factors, and can achieve the effects of repairing skin barriers, resisting inflammation, relieving, efficiently promoting repair of after-sun skin and the like.

The invention relates to the technical field of microorganisms, in particular to a plant lactobacillus BD7807 and application thereof in treating lipid metabolism disorder, and particularly discloses a plant lactobacillus BD7807 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.33630. The invention further discloses a preparation method of the plant lactobacillus BD7807. The plant lactobacillus BD7807 has obvious lipid-lowering, anti-oxidation and adhesion capabilities, is bacteriostatic, has no multiple drug resistance, can play a role in lowering lipid without damaging other organs, and can be widely used for preventing and treating related symptoms caused by lipid metabolism disorder.

The invention discloses a medicinal and edible homologous compound based on probiotic fermentation synergy and application of the medicinal and edible homologous compound in blood sugar regulation and control, and belongs to the technical field of microorganisms and fermentation engineering. The preparation method comprises the following steps: respectively carrying out two-stage fermentation on aloe, mulberry leaves and liquorice by utilizing saccharomyces cerevisiae BLCC4-0032 and plant lactobacillus DFR-1, so as to obtain a fermentation mixture; the preparation method comprises the following steps: fermenting a fermentation mixture, adding balsam pear peptide into the fermentation mixture to obtain a medicinal and edible compound, forming a metabolic closed loop, and cooperatively regulating a'blood sugar-liver-intestinal flora axis', thereby providing a new solution for diabetes intervention, and opening up a new path for preventing non-alcoholic fatty liver and intestinal inflammation caused by glucose and lipid metabolism disorder.

The invention belongs to the technical field of biological medicine, and particularly relates to red ginseng fermentation broth and a preparation method and application thereof.The preparation method of the red ginseng fermentation broth comprises the following steps that red ginseng powder is dissolved in water, and a red ginseng fermentation medium is obtained by breaking cell walls in red ginseng; mixing the seed solution of the plant lactobacillus and the seed solution of the fermentation mucus lactobacillus to obtain a compound strain seed solution; inoculating the compound strain seed solution into a red ginseng fermentation culture medium for fermentation culture to obtain a fermentation solution; centrifuging the fermentation liquor, collecting supernate, extruding and filtering the supernate, and collecting filtrate, so as to obtain the red ginseng fermentation liquor. According to the invention, compound strain fermentation is adopted, and a mechanical extrusion mode is combined to increase the content of total saponins of ginseng. According to the method disclosed by the invention, the concentration of the total saponins of ginseng is increased from 2.19 mg / mL to 7.53 mg / mL.

The invention belongs to the technical field of biological agents, and particularly relates to application of plant lactobacillus BD7807 in preparation of cheese. According to the cheese prepared by taking the plant lactobacillus BD7807 as a core fermentation strain, the quality and functional characteristics of the cheese are remarkably improved. In particular, the cheese exhibits a higher water binding capacity, a stronger acid producing ability, a lower pH value, a lower fat content, and a richer level of taste. The characteristics not only optimize the texture and flavor of the cheese, but also endow the cheese with higher nutritional value and health attribute. Therefore, the method has important practical significance in promoting the upgrading of cheese products and meeting the requirements of consumers on high-quality and functional dairy products, and shows a wide market application prospect.

The invention discloses lactobacillus mucus HG002 and application thereof, and belongs to the field of microbial medicine. The lactobacillus mucilaginosus HG002 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.36670, and the 16S rDNA gene sequence of the lactobacillus mucilaginosus HG002 is as shown in SEQ ID NO: 1. The strain is separated from excrement of healthy adults, the relieving effect of ALF is remarkably improved through the brand-new fermentation lactobacillus mucus HG002 strain and derivative EVs thereof in combination with a synergistic effect mechanism and optimized product design, many defects in the prior art are overcome, and wide application prospects and industrialization value are achieved.

The present invention relates to the technical field of probiotics, and in particular to a strain of Lactobacillus casei ZKLc1221, its lactobacillus and its application in improving ulcerative colitis. Lactobacillus casei )ZKLc1221 has been deposited in the General Microbiology Center of China Microorganism Culture Collection Committee on June 21, 2023, with the deposit address at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC No. 27673. The lactobacillus obtained by optimizing the fermentation conditions of Lactobacillus casei ZKLc1221 provided by the present invention has good thermal stability, good effect in improving ulcerative colitis, and can significantly improve pathological damage to the colon.

This application relates to the field of food microbiology, and specifically discloses a Lactobacillus plantarum capable of producing 5-methyltetrahydrofolic acid, its fermentation method and application. A Lactobacillus plantarum inm30-LP capable of producing 5-methyltetrahydrofolic acid, with a preservation date of December 26, 2022, and the preservation unit is the General Microbiology Center of the China Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC No. 26278. This application is based on the milk curd in the Gannan Tibetan area as the food source, isolates probiotics, and screens out Lactobacillus plantarum with high folic acid production from them. Moreover, the strain has obvious advantages over other lactic acid bacteria in terms of acid resistance and bile salt resistance, is suitable for growing in the gastrointestinal environment, has the ability to proliferate, has no antibiotic resistance, has antibacterial activity, and can produce bacteriocin.

The invention discloses radix ophiopogonis powder for relieving anxiety and improving sleep. The radix ophiopogonis powder is prepared by carrying out fermentation on radix ophiopogonis through plant lactobacillus GDMCC No. 64374 and carrying out enzymolysis treatment through pullulanase. The invention further discloses a preparation method and application of the radix ophiopogonis powder. The saponin content of the radix ophiopogonis powder is obviously increased. The radix ophiopogonis powder can be prepared into products in various forms, has the effects of relieving anxiety mood and improving sleep, is high in bioavailability and free of toxic and side effects, and can be eaten for a long time. Compared with the traditional radix ophiopogonis powder, the radix ophiopogonis powder disclosed by the invention is relatively low in safety risk and more obvious in effects of relieving anxiety mood and improving sleep.

The invention discloses a combined lactobacillus salivarius D7-21 and application of the combined lactobacillus salivarius D7-21, the combined lactobacillus salivarius D7-21 is named as Ligilobacterium salivarius D7-21, and the preservation number of the combined lactobacillus salivarius D7-21 is CCTCC (China Center for Type Culture Collection) NO: M 20241905. The combined lactobacillus salivarius D7-21 has certain acid resistance and salt resistance, and can still grow in an MRS culture medium with the pH value of 3; the strain can grow in an MRS culture medium added with 4g / L cholate, and can grow in an MRS culture medium added with 8% by mass of NaCl; the additive and the feed for promoting the intestinal development of the chicks both contain the saliva combined lactobacillus D7-21, and have the effect of promoting the intestinal development of the chicks.

The invention relates to plant lactobacillus and application thereof. Wherein the gene of the molecular marker for the plant lactobacillus has a nucleotide sequence selected from one of the following sequences: (1) a nucleotide sequence as shown in SEQ ID NO: 24; (2) a nucleotide sequence having at least 97%, 98%, 99% or higher homology with the nucleotide sequence as shown in SEQ ID NO: 24; and (3) a nucleotide sequence with one or more nucleotide sequences, such as 1, 2, 3, 4, 5 or more nucleotide substitutions, deletion or insertion, in the nucleotide sequence as shown in SEQ ID NO: 24. The plant lactobacillus provided by the invention is non-toxic, has good biological characteristics, has a good curative effect on prevention and / or treatment of vaginal infection and related diseases, and avoids the problems of increased drug resistance, high recurrence rate and the like caused by use of antibiotic and other antibacterial drugs during treatment of vaginal infection and related diseases.

The invention belongs to the technical field of skin care products, and particularly relates to a composition for treating acne and a preparation method thereof. The composition comprises the following components: 1-2% of a lactobacillus fermentation product and 5-8% of a plant extract component, the plant extraction component consists of a Pinus densiflora leaf extract, a celery root extract and an aster extract. The mass ratio of the Pinus densiflora leaf extract to the apium graveolens root extract to the aster extract is 1: (1-3): (5-10). Wherein the apium graveolens root extract, the Pinus densiflora leaf extract and the aster extract have a certain synergistic effect in the formula of the composition, so that the composition for treating acne provided by the invention can effectively inhibit the growth of propionibacterium acnes, reduce the occurrence of skin inflammation and effectively treat acne, and has a relatively good market application prospect.

The invention discloses a method for extracting PN / PDRN from lactobacillus bulgaricus and application of the PN / PDRN in relieving and anti-inflammatory aspects, belongs to the technical field of biochemistry, and solves the problems that in the prior art, the acquisition source of PN / PDRN is unstable, and pollution easily exists. According to the preparation method, cell walls of lactobacillus bulgaricus are preliminarily dissolved through alkaline conditions and lysozyme in the preparation process, cell membranes of bacteria are destroyed through lauryl sodium sulfate to be split, the conditions are mild, meanwhile, PVP is added to protect a PN structure, and the purity of the PN / PDRN is effectively improved through a final product prepared through the preparation method.

The invention provides a composition for preventing and treating oral ulcer and halitosis and a preparation method of the composition. The composition is prepared from the following raw materials in parts by weight: osmanthus fragrans leaching liquor, fresh rehmannia root leaching liquor, lactobacillus fermentation liquor, carbomer homopolymer A, polysorbate and auxiliary materials. The fresh rehmannia root is subjected to microbial fermentation, the content of effective substances is measured, and the composition for preventing and treating the oral ulcer and the halitosis prepared by the method is simple in component, safe and non-irritating to the oral cavity, has the application effects of efficiently resisting bacteria and removing the halitosis, can meet the requirements of people needing the composition, and has a good market prospect.

The invention relates to the technical field of microorganisms, in particular to space breeding combined lactobacillus salivarius ZK-88-S and application thereof. The combined lactobacillus salivarius ZK-88-S disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC) on December 24, 2024, and the preservation number is CGMCC No.33181; the combined lactobacillus salivarius ZK-88-S can inhibit escherichia coli, staphylococcus aureus, candida albicans, pseudomonas aeruginosa, fusobacterium nucleatum, porphyromonas gingivalis, streptococcus mutans, streptococcus salivarius, streptococcus gordoni or actinomyces naessii, and can be applied to preparation of medicines, daily necessities or health-care foods for oral treatment and oral cleaning.

The invention relates to a plant lactobacillus with effects of dispelling effects of alcohol, protecting the liver and restoring consciousness and application of the plant lactobacillus. The plant lactobacillus is a plant lactobacillus BGI-J9 strain, and the preservation number of the plant lactobacillus is CCTCC NO: M 20242630. The invention further discloses a preparation method of the plant lactobacillus with the effects of dispelling effects of alcohol, protecting the liver and restoring consciousness and application of the plant lactobacillus with the effects of dispelling effects of alcohol, protecting the liver and restoring consciousness. The strain has excellent tolerance to gastric acid, intestinal juice and cholate in a gastrointestinal tract system, can survive in the gastrointestinal tract, and provides a basis for continuously playing a probiotic effect; the compound has excellent inhibition capability on common intestinal pathogens; free radicals can be removed, and oxidative damage to the body is reduced; good alcohol metabolism enzyme synthesis capability and alcohol metabolism promoting potential are realized; the strain also has an excellent improvement effect on alcohol-damaged mice, specifically, the metabolism of ethanol and acetaldehyde in a body is accelerated, the occurrence time of drunkenness is obviously delayed, and the sobering time is shortened; intestinal injury and systemic inflammation caused by alcohol are improved; liver oxidative stress caused by alcohol is improved, and the occurrence and development process of alcoholic fatty liver is delayed; and cognitive function impairment caused by alcohol is improved.

The invention discloses high-activity plant lactobacillus freeze-dried powder and a preparation method and application thereof.According to the method, viable bacteria of plant lactobacillus fermentation liquor are increased to 1.66 * 10 < 10 >-1.82 * 10 < 10 > CFU / mL through a high-density fermentation and one-time material supplementing technology, the technology is low in production cost and easy to operate, and the problem that the viable bacteria number of the plant lactobacillus fermentation liquor is low is effectively solved. The freeze-drying protective agent is optimized by adding the plant peptide, the animal peptide and the polyphenol into the basic protective agent, the viable count of the plant lactobacillus freeze-dried powder can be increased to 1.25 * 10 < 12 >-2.55 * 10 < 12 > CFU / g, and the key problem of preparation of high-activity probiotic powder is effectively solved. At the same time, the addition of the active substances enriches the nutrition and efficacy of the freeze-dried powder, and provides a certain experimental basis for the development and application of plant lactobacillus functional food.

The invention discloses an anti-aging herba epimedii extract. The anti-aging herba epimedii extract is prepared by performing microbial fermentation on herba epimedii powder. The invention further discloses a preparation method of the anti-aging herba epimedii extract and application of the anti-aging herba epimedii extract in preparation of anti-aging drugs, food and health care products. According to the invention, through special plant lactobacillus and a fermentation process, efficient conversion of main glycoside components in herba epimedii is realized, the contents of icariin and epimedin C are reduced, the contents of secondary glycoside I and secondary glycoside II are remarkably increased, and new icaritin is generated. The herba epimedii extract disclosed by the invention has the effects of resisting aging and prolonging the service life.

The invention discloses a plant lactobacillus and an application thereof in fermentation of sophora flowers for relieving hyperuricemia. The name of the lactobacillus plantarum is lactobacillus plantarum WH-JS4, the lactobacillus plantarum WH-JS4 is preserved in Guangdong Microbial Culture Collection Center on November 7, 2024, and the preservation number of the lactobacillus plantarum WH-JS4 is GDMCC No: 65437, the GDMCC No: 65437. The lactobacillus plantarum WH-JS4 is named as lactobacillus plantarum WH-JS4. The lactobacillus plantarum WH-JS4 is named as lactobacillus plantarum WH-JS4. The lactobacillus plantarum WH-JS4 is named as lactobacillus plantarum WH-JS4. The plant lactobacillus WH-JS4 can be used for sophora flower fermentation, sophora flower fermentation liquor obtained through fermentation has a good uric acid reducing effect, and the plant lactobacillus WH-JS4 can be used for developing functional products for preventing or treating hyperuricemia.

According to the preparation method disclosed by the invention, the whole fruit juice of the lonicera caerulea (which retains fruit residues) is fermented by virtue of the plant lactobacillus KLDS1.0391, KLDS1.0320 or KLDS1.0368, so that the xanthine oxidase (XO) inhibition activity is remarkably improved. After fermentation, the XO inhibition rate is increased to 48-50.87% (the content of unfermented juice is 31.21%), the total phenol content is increased by 15-24.3%, and the contents of main XO inhibitors in the lonicera caerulea: cyanidin-3-O-glucoside (IC50 = 115.28 [mu] M), cyanidin-3, 5-diglucoside (IC50 = 116.34 [mu] M), peonidin-3-glucoside (IC50 = 105.34 [mu] M), quercetin (IC50 = 88.75 [mu] M), catechin (IC50 = 110 [mu] M) and caffeoylquinic acid (IC50 = 250.35 [mu] M) are remarkably increased. Wherein the quercetin content is increased by 73.8% at most (KLDS1.0320 strain fermentation is performed for 24 hours), and the XO inhibition rate is increased from 31.21% to 50.87%. Technical support is provided for development of a natural XO inhibitor and a functional fermented beverage, so that a new thought is provided for functional fermented juice with lonicera caerulea as a key component.

The invention discloses application of fermented lactobacillus mucus capable of prolonging life, resisting aging and resisting oxidation, and belongs to the technical field of functional strains. The preservation numbers of the lactobacillus mucus A21224 and the lactobacillus mucus A21227 are GDMCC No: 62958 and GDMCC No: 62962 respectively, and the lactobacillus mucus A21224, the lactobacillus mucus A21227 and the lactobacillus mucus A21227 are used for The two strains can remarkably prolong the service life of nematodes, have good antioxidant effect and heat stress resistant effect, can prolong the service life of the nematodes by 40% in a strong oxidation environment, and can prolong the service life of the nematodes by 28.57% under the stress of a thermal environment. In addition, the lactobacillus mucus A21224 and the lactobacillus mucus A21227 both show an excellent anti-aging effect in the late stage of the nematodes. Meanwhile, the two strains have certain gastrointestinal fluid-resistant and cholate-resistant effects, can be colonized in intestinal tracts, have no hemolysis phenomenon and are high in safety.

The invention discloses a treatment method for fermenting smelly mandarin fish by coupling gradient salt control and directional inoculation, which comprises the following steps of: pretreating the smelly mandarin fish, putting the smelly mandarin fish into ozone-containing water for bacteria reduction treatment, then putting the smelly mandarin fish into a salt solution for pickling, finally inoculating bacteria liquid into the pickled smelly mandarin fish for fermentation treatment, cleaning, draining, packaging and quickly freezing after fermentation is finished, and thus obtaining the smelly mandarin fish. According to the method, the fermentation production process of the smelly mandarin fish is adjusted and standardized, the microorganism content of the slaughtered smelly mandarin fish is reduced through pretreatment, then the bacteria-reduced smelly mandarin fish is pickled with a low-concentration sodium chloride aqueous solution, meanwhile, the screened strains (psychrobacter halophilus and lactobacillus plantarum) are inoculated, and compared with traditional fermentation, the method is easy to operate, low in cost and high in yield. The product quality is stable, the defective rate is low, the labor cost is saved, the speed is high, the volatile basic nitrogen of the obtained smelly mandarin fish is less than or equal to 30mg / 100g, and the peroxide value (in terms of fat) is less than or equal to 2.5 g / 100g.

The invention belongs to the technical field of medical preparations for pets, and particularly relates to an aloe extract-probiotic composite hydrogel for pet dermatosis, a preparation method comprises the following steps: under an aseptic condition, animal combined lactobacillus ZYpet-012 with the preservation number of CGMCC No.32008 is subjected to solid slant culture medium recovery and liquid culture, and then the animal combined lactobacillus ZYpet-012 with the preservation number of CGMCC No.32008 is subjected to freeze-drying to obtain the aloe extract-probiotic composite hydrogel for pet dermatosis. Centrifugally washing to obtain a probiotic suspension; the preparation method comprises the following steps: preparing blank hydrogel from carbomer 940, triethanolamine, zinc hyaluronate and the like; and mixing the aloe extract solution with the blank hydrogel, and then adding the probiotic suspension to prepare the composite hydrogel. According to the present invention, by using the anti-inflammatory, antibacterial and prebiotic characteristics of the aloe extract and combining the skin micro-ecology adjusting effect of the animal and the lactobacillus ZYpet-012, the prepared composite hydrogel can effectively treat the skin diseases of pets, and has advantages of nature, safety, accurate targeting and the like.