11results about How to "Increased proliferation" patented technology

The tissue repair preparation at least comprises ECM, exosome and degradable glue, the ECM is an acellular matrix such as SIS, SIM and UBM, is granular and is injectable micro powder, and the content of the ECM is 10-50 mg / ml; the exosome is a healing-promoting cell, and is obtained by induction, polarization culture, centrifugation, supernatant collection and concentration and purification. The degradable glue is a polysaccharide with viscoelasticity; the advantages of a conventional filling agent are reserved, exosomes can be slowly released and internalized by various tissue cells, the activity of fibroblasts can be stimulated, the effect is lasting, and generation of new collagen is promoted; the defects of short effect and non-lasting effect of the existing filling agent are overcome; the product provided by the invention can achieve the effects of promoting rapid regeneration of collagen and maintaining for a long time; the functions of filling, face-lifting, tissue repairing and the like are achieved; the effect is remarkable, stable and lasting; the composition has multiple functions of quick effect, obvious effect and slow release and long-acting effect.

The present invention relates to use of cell-penetrating peptide variants for wound healing. Cell-penetrating peptides according to the present invention have the effect of improving cell permeation, cell proliferation, cell migration and blood vessel formation, and thus can be used for cell-penetrating delivery, wound healing, and angiogenesis promotion. Particularly, the cell-penetrating peptides have an excellent effect of treating diabetic wounds (ulcers), which are intractable, and thus can be effectively used for wound healing.

Provided are a bispecific antibody and a use thereof. The bispecific antibody includes an IL-21 molecule and a CD16a antibody. The CD16a antibody includes a CD16a single-chain antibody and FC. The N-terminus of the FC is linked to the C-terminus of the single-chain antibody. The CD16a single-chain antibody includes a heavy chain variable region and a light chain variable region. The C-terminus of the FC is linked to the N-terminus of the IL21 molecule. The prepared bispecific antibody can simultaneously target CD16 and IL-21 receptors and can also activate NK cells. It has a long half-life period, exhibiting stronger anti-tumor and antiviral capabilities than single-target antibodies. It has the application potential of promoting NK cell proliferation and can be used for in-vitro culture and expansion of NK cells, and the expanded NK cells have good cytotoxicity.

The present invention relates to the field of medical materials, and discloses an electrolyte for micro-arc oxidation and its application, an orthopedic implant material and its preparation method. The electrolyte of the present invention comprises phosphate, strong alkali and water. The coating prepared on the surface of zinc alloy by the micro-arc oxidation electrolyte of the present invention has a zinc oxide coating with high porosity and three-dimensional structure, which not only significantly improves the corrosion rate of zinc-based materials, but also can improve the adhesion and proliferation of osteoblasts, and promotes the application of medical zinc-based materials in the preparation of orthopedic implants.

The invention discloses a culture medium and method for establishing a dog colorectal cancer type organ and application, and belongs to the technical field of pet medicines.The formula of the culture medium for establishing the dog colorectal cancer type organ comprises DMEM / F12, Gastrin, B27, N2, N-acetatecyine, GlutaMAX, antibiosis / antimycosis, amphoricin B, SB202190, Y-27632, EGF, FGF, Wnt3A, Rgasket 1 and Norggin, the formula of the culture medium for establishing the dog colorectal cancer type organ is used for establishing the dog colorectal cancer type organ, the species of recombinant proteins EGF, FGF, Wnt3A, Rgasket 1 and Norggin are dog sources, and the species of recombinant proteins FGF, Wnt3A, the formula of the dog colorectal cancer organoid culture medium is suitable for the fields of precise medical treatment, drug screening and tumor organoid of pet dogs.

This invention discloses a collagen-based anti-corrosion coating with tissue repair properties, its preparation method, and its applications, belonging to the field of medical coating technology. This invention designs a novel self-healing anti-corrosion coating and loads collagen onto the coating using excipients, achieving a deep integration of anti-corrosion protection and biological function. The prepared collagen-based anti-corrosion coating is firmly bonded to a magnesium alloy substrate via covalent bonds. After 4 weeks of in vivo implantation, no cracking or detachment was observed, the stent structure maintained good integrity, and it provides continuous radial support, offering sufficient time for vascular repair. This provides a feasible solution for the clinical translation of next-generation biodegradable magnesium-based vascular stents.

The invention belongs to the technical field of biological medicines, discloses an application of a Sema3E inhibitor in preparation of a medicine for preventing, delaying or treating COPD small airway fibrosis, and proposes that the Sema3E inhibitor can be used for treating, preventing or delaying the COPD small airway fibrosis for the first time. The Sema3E inhibitor plays a role by inhibiting the expression of Sema3E. The specific molecular mechanism is as follows: by inhibiting the expression of Sema3E, inhibiting the expression of Wnt5a / b, beta-Catenin, increasing the expression of p-beta-Catenin, inhibiting the activation of a Wnt / beta-Catenin pathway, and inhibiting the activation / proliferation / migration of small airway fibroblasts. Through the mechanism, the effect of preventing, delaying or treating the COPD is achieved, and a new drug intervention target, namely Sema3E, is provided for treatment of the COPD.

The invention discloses a complete mixing continuous flow anaerobic ammonia oxidation sludge reinforced granulation device and method, and belongs to the technical field of wastewater biological denitrification. The device takes a complete mixing continuous flow reactor as a core, and is provided with a monitoring control platform, a temperature control device, a pH regulating agent tank and a nutrient solution agent tank. A magnetic suspension stirring device is arranged at the bottom of the reactor and comprises a stirring rotor arranged in an inner cavity of the reactor and a magnetic suspension motor fixed to the outer side of the bottom of the reactor, the magnetic suspension motor drives the stirring rotor to rotate in a magnetic coupling mode without a penetrating shaft, and a strict anaerobic and high-shear mixing environment is constructed for anaerobic ammonium oxidation bacteria. A detachable sludge blocking baffle is arranged on an effluent weir at the upper part of the reactor, and sieve plates with different apertures can be replaced according to operation stages, so that efficient interception and backflow of granular sludge with different particle sizes under a continuous flow condition are realized. On-line monitoring and automatic control are integrated, the anaerobic ammonia oxidation sludge granulation process is effectively promoted, and it is guaranteed that the system stably runs for a long time under the continuous flow condition.

The invention provides a composition for preventing bone loss caused by cold exposure as well as a preparation method and application of the composition, and belongs to the technical field of pharmaceutical compositions. The composition provided by the invention is prepared from the following components in parts by weight: 1.18 to 1.58 parts of radix angelicae sinensis extract, 1.18 to 1.58 parts of flos carthami extract, 1.18 to 1.58 parts of semen persicae extract and 2.56 to 2.96 parts of fructus crataegi extract. The preparation method of the composition comprises the following steps: weighing the polymer carrier, adding the polymer carrier into precooled purified water, and adding ethanol; weighing a raw material mixture, and adding the raw material mixture into the polymer solution; performing spray drying to obtain fine powder, and performing vacuum drying to obtain the composition for preventing bone loss caused by cold exposure. The composition for preventing bone loss caused by cold exposure, prepared by the invention, has high solubility and high dissolution rate, and active ingredients take effect quickly; the bone loss caused by cold exposure can be prevented.

The invention relates to the technical field of biology, in particular to application of a CDH18 gene serving as a target spot to screening of drugs for preventing or treating cervical cancer, the drugs take the CDH18 gene as the target spot, expression of the CDH18 gene is inhibited or silenced, the proliferation and migration capacity of cervical cancer cells can be enhanced by regulating and controlling a Wnt pathway through overexpression of the CDH18 gene, and the drugs for preventing or treating cervical cancer can be used for preventing or treating cervical cancer. The down-regulation of the CDH18 gene inhibits the expression of beta-catenin, the knock-down of the CDH18 gene can inhibit the proliferation and migration ability of cervical cancer cells by regulating and controlling a Wnt signal pathway, the kaempferide has a certain potential anti-tumor activity effect in the cervical cancer cells, the kaempferide can induce the apoptosis of SiHa and HeLa cells of cervical cancer, and the kaempferide can inhibit the proliferation and migration ability of the cervical cancer cells. Experiments show that the kaempferide inhibits the expression level of Wnt signal pathway related molecules by reducing the expression level of CDH18, further promotes apoptosis of cervical cancer cells and inhibits proliferation and migration of the cervical cancer cells.

The present invention relates to the field of cell culture technology, and in particular to a CIK cell culture method and application thereof. The culture method comprises the following steps: (1) isolating peripheral blood mononuclear cells (PBMC); (2) preparing culture medium A; (3) preparing culture medium B; (4) resuspending and inoculating peripheral blood mononuclear cells; and (5) collecting cell culture. The culture method of the present invention can improve the yield of CIK cells, and wherein CD3 + CD56 + The proportion of double-positive cells is high, which increases cell proliferation activity and promotes the increase of CIK cell proliferation and killing activity.