56results about How to "Increased proliferation" patented technology

The invention discloses a pig chimeric embryo culture medium and application thereof in effectively improving the in-vitro chimeric ability of pig embryonic stem cells, and belongs to the technical field of bioengineering. According to the pig chimeric embryo culture medium disclosed by the invention, a neutral culture environment for promoting synchronous development of stem cells and embryos is created by accurately regulating and controlling growth factors, nutrient substances and other key components in the culture medium. On the basis, overexpression of anti-apoptosis factors, optimization of microinjection conditions and adoption of a laser-assisted incubation means are combined, so that the chimeric efficiency is further improved, integration of the stem cells and embryos is effectively supported, the chimeric efficiency is improved, and a reliable in-vitro tool is provided for evaluating the chimeric ability and pluripotency of the pig stem cells.

The invention relates to the technical field of skull repair, and discloses a personalized 3D printing porous composite hydroxyapatite coating skull repair body which comprises a truss structure, a plurality of porous areas are distributed on the truss structure, bionic bone trabecula structures are integrally formed in the porous areas, the truss structure is composed of hollow pipes communicating with one another, and the hollow pipes communicate with one another. A plurality of small holes and micro holes which are communicated with the interior of the hollow pipe are formed in the hollow pipe, and a composite coating of a hydroxyapatite coating and titanium is sprayed on the porous area; the invention provides a personalized 3D printed skull restoration with a porous composite hydroxyapatite coating. The problem that the biocompatibility, the bone induction capability and the bone ingrowth effect of an existing skull restoration need to be improved is solved.

The invention discloses hyaluronic acid / alginic acid dual-network hydrogel as well as a preparation method and application thereof. The hyaluronic acid / alginic acid double-network hydrogel comprises a first network and a second network which are not crosslinked with each other, and the first network and the second network are distributed in the hydrogel in an interpenetrating manner; the first network comprises an ionic cross-linked network of alginic acid with cell adhesion sites and calcium ions, and the second network comprises a chemical cross-linked network formed by covalent cross-linking of methacrylic anhydride modified hyaluronic acid. The hyaluronic acid / alginic acid double-network hydrogel disclosed by the invention is short in curing time, supports three-dimensional spreading of cells and formation of capillary lumens, has good mechanical stability, and has good application prospects in the aspects of three-dimensional culture of the cells, tissue engineering construction and the like.

The invention relates to the technical field of biological medicine, in particular to the field of cell therapy, and particularly relates to application of STAR and immune cells comprising the STAR to treatment and / or prevention of tumors, an antigen binding region of the STAR comprises one or more than two of an antibody, a ligand or an antibody fusion body which binds to LILRB4, the antibody such as scFv or VHH, and the ligand and the antibody fusion body can be combined with the LILRB4. Wherein the LILRB4 is used as a tumor antigen and / or a receptor in an immunosuppressive active cell. According to the invention, the targeted killing effect of immune cells on tumors is enhanced by targeting tumor cells and / or reducing immune escape caused by immunosuppressive active cells.

The invention discloses soothing and repairing essence lotion containing rhizoma corydalis root extract and a preparation method of the soothing and repairing essence lotion. The essence lotion is prepared from a component A, a component B, a component C and a component D. The component A is prepared from, by weight, 1.01%-3.04% of humectants, 0.23%-0.48% of thickening agents, 0.05%-0.20% of beta-glucan and 70.00%-77.00% of deionized water. The component B is prepared from the following raw materials in percentage by weight: 0.50 to 1.50 percent of butyrospermum parkii butter, 1.00 to 3.00 percent of tridecyl trimellitate, 0.15 to 0.36 percent of polydimethylsiloxane, 0.60 to 0.90 percent of glycerol tri (ethylhexoic acid) ester, 0.43 to 0.58 percent of simmondsia chinensis seed oil and 0.02 to 0.06 percent of phytosterol oleate; the component C is prepared from the following components in percentage by weight: 1.10 to 2.50 percent of an emulsifying agent, 0.40 to 0.60 percent of an antioxidant, 3.00 to 5.00 percent of butanediol, 0.15 to 0.45 percent of fibronectin, 0.30 to 0.70 percent of 1, 2-hexanediol and 10 to 13 percent of deionized water; and the component D comprises the following components in percentage by weight: 0.03%-0.10% of a neutralizing agent and 1.50%-3.50% of an anti-allergic agent. According to the invention, the rhizoma corydalis root extract is used as a main active component and is prepared into the essence lotion with the effects of relieving skin and repairing sensitive skin together with other auxiliary materials.

The invention provides a functional rare earth growth factor and a preparation method and application thereof, the rare earth growth factor comprises PEG-MoS2 and nano cerium oxide particles CeO2-NPs in a mass ratio of 1: (0.2-5), the PEG-MoS2 is a solid phase material with the surface modified with PEG, and the solid phase material is MoS2. The two materials of PEG-MoS2 and CeO2NPs are compounded, the rare earth growth factor obtained after compounding can effectively remove active oxygen existing in a wound, and the wound healing rate is effectively increased.

The tissue repair preparation at least comprises ECM, exosome and degradable glue, the ECM is an acellular matrix such as SIS, SIM and UBM, is granular and is injectable micro powder, and the content of the ECM is 10-50 mg / ml; the exosome is a healing-promoting cell, and is obtained by induction, polarization culture, centrifugation, supernatant collection and concentration and purification. The degradable glue is a polysaccharide with viscoelasticity; the advantages of a conventional filling agent are reserved, exosomes can be slowly released and internalized by various tissue cells, the activity of fibroblasts can be stimulated, the effect is lasting, and generation of new collagen is promoted; the defects of short effect and non-lasting effect of the existing filling agent are overcome; the product provided by the invention can achieve the effects of promoting rapid regeneration of collagen and maintaining for a long time; the functions of filling, face-lifting, tissue repairing and the like are achieved; the effect is remarkable, stable and lasting; the composition has multiple functions of quick effect, obvious effect and slow release and long-acting effect.

The invention relates to the technical field of cell biology, and particularly discloses a goat embryonic stem cell culture system, a system establishment method and application, the goat embryonic stem cell culture system takes mTeSRTM Plus as a basic culture solution, and 20 ng / mL to 25 ng / mL of leukemia inhibition factors, 1 mu M to 1.5 mu M of PD0325901, 4 mu M to 5 mu M of IWR-1, 45 mu g / mL to 50 mu g / mL of Vc, 15 ng / mL to 20 ng / mL of activin A, 1 mu M to 1.2 mu M of CGP77675 and 1.5 mu M to 2 mu M of GO6983 are added. Cell clone with clear edges is obtained through culture of a goat embryonic stem cell culture system, stable passage can be achieved, and the cell clone has the potential of being differentiated into three germ layers.

The invention provides a method and system for accelerating cell differentiation based on terahertz irradiation of nerve cells, and relates to the technical field of terahertz, and the method comprises the following steps: building a working platform for terahertz irradiation of nerve cells; terahertz is utilized to irradiate target nerve cells based on the working platform. The method provided by the invention can regulate the proliferation and differentiation of nerve cells, accelerate the differentiation of nerve cells and enhance the connection between nerve cells by using a proper dose of irradiation stimulation. The nerve cells can be promoted to differentiate after being irradiated by a proper dose of terahertz, so that the connection between the nerve cells is enhanced and increased, and a certain intervention effect on nervous system diseases is achieved. Therefore, along with the continuous development of cognitive neuroscience in recent years, many non-drug nerve intervention means are generated, and the method for regulating the differentiation of the nerve cells by adopting the appropriate dosage of terahertz wave stimulation is an effective and reliable way for enhancing and increasing the connection between the nerve cells and intervening the faster and better development of the treatment of nervous system diseases.

The present invention relates to the field of molecular biology, and in particular to a biomarker related to liver cancer and its application. The present invention first discovered that the expression of CYSTM1 gene is upregulated in liver cancer, and the total survival of liver cancer patients with high expression of CYSTM1 gene is significantly reduced, suggesting that detecting the expression level of CYSTM1 gene can become one of the auxiliary diagnostic indicators for liver cancer diagnosis, and high expression of CYSTM1 gene has high accuracy as a liver cancer diagnostic factor. Moreover, the present invention also discovered that inhibiting the expression of CYSTM1 gene can effectively inhibit the proliferation and cloning of liver cancer, and overexpressing CYSTM1 gene can significantly enhance the proliferation and cloning of liver cancer. Therefore, interfering with the expression of CYSTM1 gene can become a new way to prevent or treat liver cancer, providing a new idea for the development of related drugs and clinical treatment in the later stage.

The invention provides a carbonyl manganese polydopamine composite scaffold as well as a preparation method and application thereof. Polyethylene glycol-block-polypropylene glycol-block-polyethylene glycol diacrylate, dopamine hydrochloride, 1, 3, 5-trimethylbenzene, ammonium hydroxide and carbonyl manganese react to prepare a carbonyl manganese polydopamine compound, and then the carbonyl manganese polydopamine compound is loaded on mineralized decellularized lotus stems to prepare the carbonyl manganese polydopamine composite scaffold. By applying the composite scaffold, the bone differentiation and regeneration capability can be effectively enhanced.

The present disclosure relates to the field of biomedicine, and specifically relates to a bispecific NK cell agonist, a preparation method, and a use. The present disclosure provides a fused protein. The fused protein uses a CD16A receptor and a 4-1BB receptor as targets to obtain a new bispecific NK cell agonist medicament, i.e., scfv-CD16A-Trimer-4-1BBL; the medicament has the activity of activating and amplifying an NK cell in vitro, enhancing the antivirus and antitumor capabilities of the NK cell, and has the potential effects of promoting NK cell proliferation and enhancing a T cell function, and has the application potential of culturing and amplifying the NK cells in vitro; and the amplified NK cells have good cytotoxicity.

The invention discloses an in-vitro extraction and amplification method of a vascular development related dental pulp stem cell subpopulation, which comprises the following steps: extracting primary DPSCs from a dental pulp tissue through trypsin and collagenase digestion, and preparing a single-cell suspension; sorting the cells using flow cytometry; and the PDGFR beta + DPSCs are sorted out through a flow cytometer. And finally, inoculating the separated PDGFR beta + DPSCs into a DMEM culture medium containing 10% of FBS and 1% of penicillin-streptomycin, and culturing under the conditions of 37 DEG C and 5% of CO2. The PDGFR beta + DPSCs purified and cultured through the method have high proliferation, migration and angiogenesis promoting performance. According to the method, autologous dental pulp stem cells which are from donors of different ages and under different conditions and have high angiogenesis promoting characteristics can be stably obtained in a clinical environment to a certain extent. The method provides a necessary research basis for exploring and maintaining a regulation mechanism of DPSCs self-renewal, stable amplification and promotion of vascular performance, further widening the clinical application prospect of DPSCs and promoting dental pulp regeneration treatment.

The invention discloses a culture medium, a method and an application for establishing a mammary organoid or / and a breast cancer organoid, and belongs to the technical field of biomedicine. The culture medium is a DMEM / F12 complete culture medium containing the following components: estradiol, hydrocortisone, R-Spondin 3, Neuregulin 1, FGF7, FGF10, rh-EGF, PDGF, Noggin, A83-01, Wnt3a, Y-27632, SB202190, B27, N-acetylcysteine, nicotinamide, GlutaMax, fetal bovine serum and penicillin / streptomycin double antibody. The culture medium is used for establishing a mammary organoid or / and a breast cancer organoid, can accelerate the proliferation and self-assembly ability of mammary or / and breast cancer cells, accelerate the establishment of organoids and improve the success rate of the establishment of organoids.

The invention discloses a cultivation method and a cultivation evaluation method of Amomum villosum stem tiller seedlings, and relates to the field of cultivation evaluation methods. The cultivation method comprises the following steps: adding 6-benzyladenine into a culture medium to obtain an induced budding culture medium; a tissue culture rapid propagation technology is adopted, leaves and sheaths are cut off, and stems with roots are left; and vertically inserting the stem segments into an induction budding culture medium, and culturing to obtain stem tillering seedlings. The rapid tissue propagation technology is adopted, pathogens and other viruses in the plant body can be effectively removed, the 6-benzyladenine with the high concentration is adopted to induce budding of stem segments, meanwhile, the vertical inoculation mode is combined, budding and proliferation of stem tillering seedlings are effectively improved, the seedling growing period is short, high-speed and high-yield high-quality seedlings can be achieved, and the method is suitable for large-scale popularization and application. And excellent germplasm resources required by industrial development are ensured.

The invention discloses application of quercetin in reduction of the number of resistance genes in soil, solid bacterial fertilizer and a preparation method, and relates to the technical field of preparation of solid bacterial fertilizer and reduction of the number of resistance genes. Quercetin is introduced in the fermentation process of the solid bacterial fertilizer to inhibit propagation and diffusion of resistance genes, and the ecological safety of the bacterial fertilizer is improved on the premise that growth and proliferation of beneficial microorganisms are not affected. The concentration (0.01%-0.05%) of quercetin and the adding mode (added in the first-stage fermentation) are precisely designed, and the inhibition effect of quercetin on beneficial bacteria can be effectively avoided, so that the high efficiency and safety of the bacterial fertilizer are guaranteed.

The present invention relates to use of cell-penetrating peptide variants for wound healing. Cell-penetrating peptides according to the present invention have the effect of improving cell permeation, cell proliferation, cell migration and blood vessel formation, and thus can be used for cell-penetrating delivery, wound healing, and angiogenesis promotion. Particularly, the cell-penetrating peptides have an excellent effect of treating diabetic wounds (ulcers), which are intractable, and thus can be effectively used for wound healing.

The invention discloses a probiotic hydrogen water beverage with an antioxidant function and a preparation method of the probiotic hydrogen water beverage, and belongs to the technical field of foods. The preparation method comprises the following steps: (1) mixing prebiotics, a fermentation substrate and a first part of hydrogen-rich water, and carrying out primary fermentation; (2) mixing probiotics, food auxiliary materials and a product of the first fermentation, and performing second fermentation; and (3) mixing the product of the second fermentation with a second part of hydrogen-rich water to obtain the probiotic hydrogen water beverage. The prebiotics, the lactic acid bacteria and the multi-stage activated hydrogen-rich water are organically combined, the balance of intestinal flora in the body of a consumer can be maintained and the immunity of the consumer can be enhanced after long-term drinking, and after the multi-stage activated hydrogen-rich water is added, due to the high gas diffusivity of hydrogen, the multi-stage activated hydrogen-rich water can quickly penetrate through a biological membrane to reach the interiors of cytoplasm, mitochondria and cell nucleuses, so that the immunity of the consumer is enhanced. Free radicals are effectively removed, and the effects of resisting inflammation, relieving and eliminating pain, relieving chronic diseases and promoting metabolism are achieved.

Provided are a bispecific antibody and a use thereof. The bispecific antibody includes an IL-21 molecule and a CD16a antibody. The CD16a antibody includes a CD16a single-chain antibody and FC. The N-terminus of the FC is linked to the C-terminus of the single-chain antibody. The CD16a single-chain antibody includes a heavy chain variable region and a light chain variable region. The C-terminus of the FC is linked to the N-terminus of the IL21 molecule. The prepared bispecific antibody can simultaneously target CD16 and IL-21 receptors and can also activate NK cells. It has a long half-life period, exhibiting stronger anti-tumor and antiviral capabilities than single-target antibodies. It has the application potential of promoting NK cell proliferation and can be used for in-vitro culture and expansion of NK cells, and the expanded NK cells have good cytotoxicity.

The present invention provides a degradable polymer repair material and a preparation method thereof, and relates to the technical field of medical biomaterials. A degradable polymer repair material, by weight, comprises: 1-10 parts of polyanhydride, 0.5-2.5 parts of calcium phosphate, 1-10 parts of lactic acid, 0.1-1 parts of polyethylene glycol, 1-10 parts of glycolic acid, 1-5 parts of biologically active liquid and 1-5 parts of antibacterial agent. The degradable polymer repair material of the present invention has special biological activity, good biocompatibility and biosafety, and also has excellent mechanical strength, chemical stability, and is biodegradable.

The invention provides a high-performance implantable biomedical product based on polylactic acid and a preparation method thereof.The preparation method comprises the steps that by weight, 10-20 parts of poly (butylene succinate-adipate) and 1-2 parts of hydroxyapatite are subjected to melt blending to prepare a PBSA / HA mixture; mixing 78-89 parts of polylactic acid with PBSA / HA, carrying out melt blending extrusion through a twin-screw extruder, and granulating to obtain PLA / PBSA / HA granules; finally, the PLA / PBSA / HA granules are prepared into the high-performance implantable biomedical product through a micro injection molding process. According to the method, in-situ fiber forming of a PBSA dispersed phase in a PLA matrix is achieved through induction of a micro injection molding process under the condition of a strong shear force field, meanwhile, the toughness of the product is unexpectedly and remarkably improved by adding a specific content of hydroxyapatite, and the prepared product has excellent biocompatibility and bone induction activity.

The present invention relates to the field of medical materials, and discloses an electrolyte for micro-arc oxidation and its application, an orthopedic implant material and its preparation method. The electrolyte of the present invention comprises phosphate, strong alkali and water. The coating prepared on the surface of zinc alloy by the micro-arc oxidation electrolyte of the present invention has a zinc oxide coating with high porosity and three-dimensional structure, which not only significantly improves the corrosion rate of zinc-based materials, but also can improve the adhesion and proliferation of osteoblasts, and promotes the application of medical zinc-based materials in the preparation of orthopedic implants.

The present invention relates to a composition for preventing or treating cancer diseases, the composition comprising CD8+T cells activated by extracellular vesicles derived from CD4+T cells as an active ingredient. It was found that the extracellular vesicles secreted from cytokine-activated CD4+T cells increased, and the extracellular vesicles enhanced the proliferation and activity of CD8+T cells to induce cancer cell death, thereby enhancing the anti-cancer effect. Therefore, the present invention provides CD8+T cells activated by extracellular vesicles derived from CD4+T cells as a medicament or immunotherapeutic agent for cancer diseases, and a method for activating CD8+T cells using extracellular vesicles derived from CD4+T cells to prepare CD8+T cells exhibiting excellent anti-cancer activity as described above.

The invention relates to the technical field of genetic engineering, in particular to a modified mesenchymal stem cell as well as a construction method and application thereof. The construction method comprises the following steps: 1) constructing an sgRNA lentiviral vector containing an Nes gene; and 2) transfecting mesenchymal stem cells with the sgRNA lentiviral vector to obtain the modified mesenchymal stem cells. According to the modified mesenchymal stem cell disclosed by the invention, through a Crispr / cas9 gene editing technology, netin is over-expressed in the mesenchymal stem cell, so that the proliferation and migration capabilities of the mesenchymal stem cell are enhanced, the proliferation of liver cells can be promoted, and the liver regeneration capability is improved. The defect that the clinical application of the mesenchymal stem cells is limited due to amplification and acquisition of the mesenchymal stem cells is overcome.

The invention relates to the technical field of immune cell culture, and discloses an immune cell culture perfusion device which comprises a base, a base arranged on the base, a drainage component arranged in the base, an atomization component arranged on the base, a lifting component arranged on the base and a protection component arranged on the atomization component. Fresh culture media are atomized through the atomizer and introduced into the culture tank, the surface area of the culture media added into the culture tank is increased, the contact efficiency of immune cells in the culture tank and the culture media is improved, meanwhile, attachment points are provided for mist particles in the atomization tank through the capture rod and the supporting rod, and the atomization effect is improved. The fog particles are condensed into a liquid state and continuously dripped into the culture tank, so that the situation that the immune cells are damaged and inactive and functional due to shearing force generated by flowing of a liquid culture medium and acting on the immune cells during culture medium perfusion is avoided, a stable and suitable growth environment is provided for the immune cells, and the immune cells are protected. And proliferation and differentiation of immune cells in the culture tank are improved.

The invention relates to the technical field of biology, in particular to application of hemocyanin (hemocyanin, HMC) of prawns. As an oxygen-carrying protein, the hemocyanin can be used as an additive for preparing a medicament for promoting cell growth. The hemocyanin provided by the invention can promote the cell cycle by repairing DNA damage so as to enhance the cell proliferation ability, and plays a role in reducing tissue damage.

The invention is suitable for the technical field of biomedicine and high polymer materials, and provides a 3D printing porous screw with a gradient structure coating and a preparation method of the 3D printing porous screw. According to the invention, a cross-linked structure with double gradient distribution is designed, and bivalent bioactive cations with different concentrations are cross-linked with a sodium alginate solution to form a hydrogel system with biomechanical stress gradient. The system not only can promote the differentiation of tissue resident stem cells and provide gradient biological stress support, but also can maintain the ion biological activity concentration in the local environment of damaged tissues through slow-release ions, and promote blood supply recovery and anti-infection ability. Besides, the biocompatibility and the mechanical property of the hydrogel are enhanced by the de-telopeptide type I collagen doped in the system, so that the porous screw has excellent stability and cell ingrowth capability, thereby better adapting to complex clinical scenes, and improving the tissue repair effect and the clinical application value of biological materials.

The invention provides application of a VISTA gene as a primary liver cancer early diagnosis marker, and belongs to the technical field of biological medicine. The invention finds that proliferation and clone formation of the liver cancer can be effectively inhibited by inhibiting expression of the VISTA gene, and proliferation and clone formation of the liver cancer can be remarkably enhanced by overexpressing the VISTA gene. The BRD4 is an upstream regulatory factor of VISTA, the protein level of VISTA is obviously increased after the BRD4 is over-expressed, and the protein level of VISTA is obviously reduced after the BRD4 is knocked down; the MMP11 is a downstream substrate of the VISTA, the VISTA regulates and controls the MMP11 at the transcriptional level and the protein level, the expression level of the VISTA is over-expressed, the expression level of the MMP11 is obviously increased, and after the VISTA is knocked down, the expression level of the MMP11 is obviously reduced. The BRD4 regulates and controls the mRNA transcription of the MMP11 by regulating and controlling the expression of the VISTA, so that the occurrence of the primary liver cancer is influenced.

The invention discloses a culture medium and method for establishing a dog colorectal cancer type organ and application, and belongs to the technical field of pet medicines.The formula of the culture medium for establishing the dog colorectal cancer type organ comprises DMEM / F12, Gastrin, B27, N2, N-acetatecyine, GlutaMAX, antibiosis / antimycosis, amphoricin B, SB202190, Y-27632, EGF, FGF, Wnt3A, Rgasket 1 and Norggin, the formula of the culture medium for establishing the dog colorectal cancer type organ is used for establishing the dog colorectal cancer type organ, the species of recombinant proteins EGF, FGF, Wnt3A, Rgasket 1 and Norggin are dog sources, and the species of recombinant proteins FGF, Wnt3A, the formula of the dog colorectal cancer organoid culture medium is suitable for the fields of precise medical treatment, drug screening and tumor organoid of pet dogs.

The present invention relates to a pharmaceutical composition for cell proliferative activity and a method for producing the same. [Problem] The purpose of the present invention is to provide a composition having a cell proliferation effect, with the purpose of effectively using waste pomegranate seeds and increasing the added value thereof. [Solution] A pharmaceutical composition for cell proliferative activity according to the present invention is characterized by containing a pomegranate seed extract. Furthermore, a preferred embodiment of the pharmaceutical composition for cell proliferative activity according to the present invention is characterized in that the pomegranate seed extract is obtained by immersing a pulverized product obtained by pulverizing pomegranate seeds in at least one solvent selected from the group consisting of ethanol, methanol, water, hexane, and the like, and separating a supernatant.