742 results about "Tissue repair" patented technology

A cannula system and method for tissue repair includes a cannula defining a central lumen and a divider removably coupled to the cannula. The divider has a divider shaft to separate the central lumen into at least two discrete working channels.

The invention discloses an oral care composition and a preparation method thereof, the oral care composition comprises a friction agent, a periodontal repair agent, a thickening agent, a humectant and a foaming agent, the friction agent comprises a dental plaque release agent, and the dental plaque release agent is configured to destroy a bacterial extracellular polysaccharide matrix in dental plaque; the periodontal repair agent comprises at least one of the following components: an inflammation inhibitor, a tissue repair promoter, a mucous membrane stimulation alleviator and a bacteriostatic agent. The oral care composition contains the dental plaque release agent and the periodontal repair agent, and can synergistically improve periodontal health.

The invention belongs to the technical field of biological materials and ultrasonic printing, and particularly discloses a biological adhesive for ultrasonic printing and preparation and application thereof. The biological adhesive comprises a heat-sensitive main body material and a chemical adhesion component, the heat-sensitive main body material comprises hydroxybutyl chitosan, and the chemical adhesion component comprises polyacrylic acid-N-hydroxysuccinimide ester. The bioadhesive has excellent ultrasonic responsiveness, formability and bioadhesion performance, directional deposition, in-situ solidification and tissue surface adhesion can be achieved in a non-contact and accurately-controlled ultrasonic printing system, and the bioadhesive is suitable for various biomedical scenes such as tissue repair, minimally invasive medicine, biological device construction and drug delivery interface printing.

The invention provides a preparation method of a shape-adaptive hydrogel material for tissue repair. The method comprises the following steps: S1, preparing HPC (Hydroxypropyl Chitosan); s2, preparation of phenylboronic acid functional chitosan PHPC (Polyhydroxyalkanoate); s3, preparing a PHPC solution; s4, preparing a polyvinyl alcohol (PVA) solution; and S5, preparing the dynamic covalent bond hydrogel. According to the invention, a dynamic covalent bond-boric acid ester bond with stimulation response capability is introduced on the basis of common dual-network CS-PVA hydrogel; on the premise of not changing the biocompatibility and biodegradability of the original dual-network hydrogel, the hydrogel is endowed with response capability under physical and chemical stimulation, and the diversity of application scenes is expanded; the dynamic mechanical behavior changes of the PCA hydrogel at four different strain rates of 0.05 mm / s, 0.5 mm / s, 5 mm / s and 50 mm / s are explored, and the passive shape adaptability of the PCA hydrogel along with tissue growth is studied; meanwhile, changes of hydrogel creep deformation and stress relaxation caused by addition of dynamic covalent bonds are researched.

The invention discloses preparation and application of dual-network hydrogel. The dual-network hydrogel comprises the following components: (Z1) a spinal cord acellular matrix network DSM formed by self-assembly of a spinal cord acellular matrix dSECM; and (Z2) a metal-polyphenol network MPN formed by coordination of metal ions and polyphenols; wherein the DSM and the MPN are cross-linked through hydrogen bonds to jointly form a double-network structure. DSM provides a microenvironment suitable for cell survival and growth for the dual-network hydrogel and induces stem cells to differentiate into neurons, and MPN provides the dual-network hydrogel with biological effects of inhibiting inflammation and accumulation of reactive oxygen species (ROS). The dual-network hydrogel has the advantages of the two components, the active component neurotrophic factor and the inhibitor loaded on the dual-network hydrogel can regulate and control immune response after SCI, improve neuroinflammation microenvironment and promote nerve repair, and a new strategy is provided for in-situ tissue repair of SCI.

The invention relates to a near-infrared light response in-situ gelling and synergistic photo-thermal-photodynamic gel delivery system and a preparation method and application thereof, a mesoporous silicon coated up-conversion nano-carrier and an efficient photosensitizer are compounded to form a photo-response core unit, and then the photo-response core unit is intelligently integrated with photo-curing hydrogel to form the near-infrared light response in-situ gelling and synergistic photo-thermal-photodynamic gel delivery system. The general problem of aggregation and quenching of photosensitive molecules is relieved by utilizing the unique space confinement effect of the mesoporous structure, the ICG photo-thermal efficiency and photodynamic performance are enhanced, under precise excitation of a near-infrared light source, the system can activate a photo-crosslinking reaction through an up-conversion luminescence process, in-situ conversion from a liquid eye drop preparation to a stable gel state is achieved, and the stability of the eye drop preparation is improved. A lasting drug reservoir is formed on the surface of the cornea; synchronously excited photodynamic and photo-thermal dual-mode treatment generates a remarkable synergistic antibacterial effect, and shows an excellent removal capability on pathogens including highly drug-resistant bacteria; meanwhile, an ideal microenvironment is created for tissue repair through high biocompatibility and a stable mechanical matrix, and integrity recovery of cornea structures and functions is promoted.

The invention provides an exosome from rice as well as a preparation method and application thereof. The exosome has a double-layer membrane spherical structure, and the particle size range is 20-1000nm. The exosome is obtained from rice calluses, and has the characteristics of high biological safety, high stability and sustainable mass production. And tests prove that the exosome has the functions of resisting aging, promoting cell regeneration and tissue repair, protecting and the like, and also can remarkably improve the cell absorption efficiency and the delivery efficiency of active ingredients such as peptide cells.

The invention relates to an OCT (Optical Coherence Tomography) navigation uterine cavity adhesion and dissection postoperative residual adhesion prediction method, which comprises the following steps of: acquiring an interference signal of an intraoperative uterine cavity wall area by utilizing OCT equipment, and identifying an adhesion fiber microstructure which is not completely broken so as to judge the existence of an optically invisible residual adhesion microbridge; applying low-frequency bioelectrical stimulation to the wounded area of the uterine cavity during the operation, and marking the wounded area as a tissue repair electrical activity silent area if no blood flow micro-reaction is continuously detected; oCT micro-deformation sequence analysis is carried out on the region after intraoperative dissection is completed, mutation breakpoints of tissue elastic distribution are identified, and the potential risk that traction after-tack occurs in the region is predicted; detecting whether a metabolic oxidation reaction residual zone exists in the operation area or not so as to judge local tissue repair activity decline and re-adhesion tendency; local micro-intervention stimulation is applied to the tissue area judged to be high in risk, OCT imaging is carried out again to detect the reversibility of tissue optical response, and if an image is still fractured and red shift is enhanced, it is judged that the area is the postoperative irreversible adhesion residual area.

The invention provides a polyethylene glycol derivative-collagen cartilage repair gel which is characterized by being prepared from the following components in parts by weight: 8 to 12 parts of collagen (namely COL) solution, 0.5 to 2 parts of polydeoxyribonucleotide (namely PDRN), 4 to 8 parts of polyethylene glycol disuccinimide succinate (namely SS-PEG-SS) solution and 0.1 to 0.4 part of tannic acid (namely TA), and particularly discloses a preparation method and application of the polyethylene glycol derivative-collagen cartilage repair gel. In a word, the composite gel and the collagen are subjected to efficient amidation reaction through SS-PEG-SS to form a stable three-dimensional network, so that the structural support of the type I collagen, the tissue repair of PDRN and the anti-inflammatory and anti-oxidation characteristics of tannic acid are successfully integrated; therefore, the composite material has the comprehensive performance of good structural stability, high mechanical strength, good biocompatibility, controllable degradation period, excellent needle passing performance and the like, and shows the wide application prospects of clear repair effect, remarkable component synergistic effect, mature production process, outstanding cost effectiveness and the like.

The invention discloses a photocuring microneedle with ginseng exosome loaded berberine, a preparation method of the photocuring microneedle and application of the photocuring microneedle to tissue repair. A ginseng exosome loaded berberine compound is prepared through a microfluidic technology; filling a needle cavity of a microneedle mold with a needle tip layer preparation solution containing the compound, methylacryloylated hyaluronic acid and methylacryloylated gelatin through a centrifugal method; covering a backing layer preparation solution composed of hyaluronic acid and povidone K90, and centrifuging again; and finally, carrying out light curing, drying and demolding. The prepared composite microneedle is complete and sharp in needle shape and high in forming rate; the efficient entrapment of the active medicine is realized; the biocompatibility is good, and the irritation is low; a cross-linked network structure formed by photocuring is beneficial to realizing controlled release of drugs, the targeted repair function of the ginseng exosome is combined with the hypoglycemic effect of berberine, and a new strategy and technical approach with a prospect are provided for painless, efficient and long-acting treatment of diabetes mellitus.

The invention belongs to the technical field of exosome freeze-dried powder, and discloses exosome freeze-dried powder and a preparation method thereof, the exosome freeze-dried powder contains mesenchymal stem cell exosome, trehalose, sodium hyaluronate, hydroxypropyl-beta-cyclodextrin, glutathione, ceramide and fructo-oligosaccharide. According to the exosome freeze-dried powder, hydroxypropyl-beta-cyclodextrin, glutathione, ceramide and fructo-oligosaccharide are added, the membrane structure integrity of the exosome is effectively protected, the retention rate of active substances in the exosome freeze-dried powder is high, and therefore the effects of resisting inflammation, regulating cell metabolism, promoting tissue repair and the like of the exosome can be fully exerted. In addition, glutathione can directly remove skin free radicals and inhibit oxidative stress injury, and has an anti-oxidation effect; ceramide can also supplement skin lipid barrier, enhance the water locking capacity and enhance the moisturizing effect of sodium hyaluronate; fructo-oligosaccharide can also regulate skin micro-ecological balance, and has anti-inflammatory and bacteriostatic effects.

The invention provides a composition containing SVF and hyaluronic acid and application of the composition to tissue repair. Furthermore, the invention obtains the angiogenesis peptide for promoting angiogenesis through screening and identification, and the polypeptide has better effects of promoting endothelial cell proliferation and angiogenesis. The composition prepared by mixing the angiogenesis peptide MA-R-5 capable of promoting angiogenesis with SVF and fat particles and adding hyaluronic acid has the advantages of better reducing the degradation of a graft and promoting the generation of new vessels around the graft, and has a wide application prospect.

The invention relates to the technical field of bionic medical materials, in particular to a bionic scaffold material for promoting repair and reconstruction of infectious bone defects and a preparation method and application of the bionic scaffold material. The preparation method comprises the following steps: wrapping the oxygen vacancy titanium dioxide nanorod in collagen through temperature control, and directionally depositing mineralized crystals in a gap region in the collagen to obtain the porous bionic scaffold with a bone-like structure. The bionic scaffold material disclosed by the invention has a bone-like structure, not only can realize efficient physical antibiosis after ultrasonic irradiation, but also can adjust the intercellular effect of macrophages to promote tissue repair, and the repair and reconstruction effects of the material on infectious bone defects are verified in animal experiments. The successful preparation of the material can realize time-space controllable precise antibiosis, further promote the repair and regeneration of bone tissues, and provide a new method for clinical treatment of bone defects, especially bone defect related diseases accompanied by infection.

The application belongs to the technical field of medical materials, and discloses a soft tissue repair patch and a preparation method thereof, which comprises the following steps: S1, treating and cleaning animal soft tissue to obtain a first intermediate product; S2, adding the first intermediate product into a reagent to perform freeze-thaw circulation to obtain a second intermediate product; S3, soaking and treating the second intermediate product with an organic solvent and cleaning to obtain a third intermediate product; S4, soaking and treating the third intermediate product with an alkali solution and cleaning to obtain a fourth intermediate product; and S5, soaking and treating the fourth intermediate product with an acid solution, cleaning, drying and sterilizing to obtain the soft tissue repair patch. The application provides a more gentle and friendly decellularization scheme, and the decellularized matrix prepared by using the scheme is more completely decellularized, has no chemical residue, and maintains the natural scaffold structure of collagen.

The invention discloses a preparation method of a flexible electronic device with specific wet adhesion capability, which comprises the following steps: dissolving acrylamide Am, methacrylic acid esterified gelatin GelMA and chitosan powder in an acetic acid solution, uniformly stirring, adding carboxylated multi-walled carbon nanotubes (MWCNTs) into the mixed solution, stirring uniformly, and drying to obtain the flexible electronic device with specific wet adhesion capability. Performing ultrasonic treatment, water bath treatment and degassing treatment to obtain a precursor solution; the preparation method comprises the following steps: mixing sodium monohydrogen phosphate and sodium dihydrogen phosphate, adding sodium bicarbonate, adding ammonium persulfate as an initiator, fully stirring, and degassing to obtain a gelling solution for inducing physical crosslinking of chitosan; selecting a material with carboxyl, amino and hydroxyl groups to manufacture a mold for polymer cross-linking molding; and mixing and stirring the precursor solution and the gelling solution, injecting into a prepared mold, placing at room temperature, and integrally forming to manufacture the flexible electronic device. The invention further discloses application of the flexible electronic device in preparation of a bioelectric sensor sensitive element and tissue repair.

A knotless tissue repair construct is disclosed herein. The construct includes a soft anchor body, with a proximal end, a distal end, and a longitudinal axis extending therebetween. The construct also includes a repair suture fixedly coupled to the anchor body. The construct also includes a transfer suture interwoven repeatedly through and along the anchor body defining a transfer path. Tension on at least one of the repair suture and transfer suture may deploy the anchor body. A transfer suture first end may transfer the repair suture through the anchor body to form a repair loop that may knotless lock the tissue repair construct.

The invention relates to a nerve repair guide catheter, a preparation method and application, an electrostatic spinning technology is adopted to construct a nanofiber membrane which is directionally and orderly arranged so as to guide nerve axons to directionally grow, and cell energy metabolism is promoted and tissue repair and regeneration are accelerated through metabolism and conversion of degradation products. Therefore, the nerve injury repairing effect better than that of traditional matrix materials without biological activity, such as PCL, is achieved. According to the invention, a novel solution is provided for solving the problem of insufficient biological activity of a traditional nerve conduit material by constructing a degradable high polymer material with biological activity. Therefore, the regeneration efficiency of peripheral nerve injury repair is improved, and the application prospect of the bioactive material in the fields of tissue engineering and regenerative medicine is expanded.

Non-woven graft materials for use in specialized surgical procedures such as neurosurgical procedures, methods for making the non-woven graft materials, and methods for repairing tissue such as neurological tissue using the non-woven graft materials are disclosed. More particularly, disclosed are non-woven graft materials including at least two distinct fiber compositions composed of different polymeric materials, methods for making the non-woven graft materials and methods for repairing tissue in an individual in need thereof using the non-woven graft materials.

The invention provides a near-infrared light mediated hybrid hydrogel multi-bioactivity synergistic tissue repair promoting material as well as a preparation method and application thereof, and relates to the technical field of biological materials. The method comprises the following steps: 1) dispersing calcium peroxide in a solvent, and adding a 3-hydroxytyramine hydrochloride solution for reaction to obtain CaO2 (at) PDA nanoparticles; enabling the CaO2 (at) PDA nano-particles to react with a catalase solution, so as to obtain ORN oxygen release particles; (2) mixing the ORN oxygen release particles with the surfaces rich in sulfydryl with Se-NPs with the surfaces rich in selenol after reduction treatment under the protection of inert atmosphere to obtain ORN-coated Se composite particles; the preparation method comprises the following steps: dissolving hyaluronic acid methacrylate in deionized water, adding the ORN-coated Se composite particles, uniformly mixing, adding a photoinitiator, and carrying out ultraviolet curing to obtain the ORN-coated Se-HAMA hydrogel which can realize local response oxygen supply on an anoxic wound surface and has antibacterial and antioxidant characteristics.

The invention discloses a composite amnion for improving contracture after tissue repair and a preparation method of the composite amnion. The composite membrane comprises a recombinant humanized collagen nanofiber membrane located in the middle layer and human decellularized amniotic membranes attached to the two faces of the nanofiber membrane. The composite membrane is prepared by preparing a recombinant humanized collagen nanofiber membrane through an electrostatic spinning technology and pressing the recombinant humanized collagen nanofiber membrane and a human decellularized amniotic membrane. The composite membrane has excellent mechanical properties and biological activity. Animal experiment results show that the composite film can effectively inhibit capsule contracture induced by radiotherapy, and the comprehensive effect of the composite film is obviously superior to that of a single-component material in the aspects of inflammation resistance, fibrosis resistance, degradation speed delaying, capsule compliance improving and the like. The composition can be used for improving the capsule contracture of the breast prosthesis, especially the capsule contracture induced by radiotherapy.

The invention relates to an adipose-derived stem cell and exosome composition for mucosal inflammation and an application of the adipose-derived stem cell and exosome composition. The composition is composed of a hybrid nano-vesicle and a pulsatilla chinensis exosome compound in a mass ratio of 1: 1. The hybrid nanovesicles are prepared by fusing adipose-derived stem cell exosomes and lipidosome and modifying honey polysaccharide, and the pulsatilla chinensis exosome compound is loaded with interleukin 23 peptidomimetic, polyethylene glycol, black scale quantum dots and camptothecin. The composition has the functions of efficient anti-inflammation, immunoregulation, precise targeting and tissue repair, and meanwhile, the curative effect is enhanced through photo-thermal synergistic treatment. The preparation method comprises the steps of exosome extraction by a density gradient centrifugation method, liposome fusion and surface modification. The composition is suitable for treating mucosal inflammation such as inflammatory bowel disease and oral mucositis, has the advantages of high stability, good biocompatibility and the like, and provides a safe and effective novel treatment strategy for clinic.

The invention provides a tissue repair filling composite gel as well as a preparation method and application thereof, and relates to the technical field of biomedical materials. The composite gel is mainly prepared from hyaluronic acid and biodegradable microspheres through freezing and thawing physical crosslinking, the molecular weight of the hyaluronic acid is 80000-3000000, and the biodegradable microspheres are hydroxyapatite microspheres. The tissue repair filling composite gel is composed of a hyaluronic acid matrix which is obtained through freeze-thaw physical crosslinking and has a porous three-dimensional network structure and hydroxyapatite microspheres uniformly dispersed in the hyaluronic acid matrix, in the filling repair process, hyaluronic acid provides an instant filling effect, the hydroxyapatite microspheres stimulate collagen regeneration, and the tissue repair effect is improved. According to the tissue repair filling composite gel, the technical problems that the safety is poor due to the fact that existing hyaluronic acid gel obtained by relying on a cross-linking agent is used for in-vivo filling, and a regenerative microsphere filling agent is inconvenient to inject and use are effectively solved.

Disclosed in the present invention are a bionic electrothermal coupling composite material based on a pyroelectric effect, a medical kit and a use. The composite material subjected to corona poling treatment of the present invention has a surface potential of about 80 mv, the composite material can generate a pyroelectric effect by loading near-infrared light in vitro, and as can be seen from a diagram showing the variation of pyroelectric current with temperature, charge release occurs during temperature increase, thereby establishing correlation between electrical and thermal properties of a material, and realizing electrothermal effect-driven bionic coupling of the material. The synergistic effect of the piezoelectric properties and the pyroelectric effect is achieved by coupling, achieving the effects of promoting macrophage polarization and promoting tissue repair or healing. In exemplary embodiments, the charge generation capability of a material can be improved by controlling near-infrared light in vitro in a non-invasive manner, on-demand power supply based on a near-infrared light external field is realized, and the problem of insufficient long-term performance of a charged material after implanted into the body is solved.

This invention provides aragonite- and calcite-based scaffolds for the repair, regeneration, enhancement of formation or a combination thereof of cartilage and / or bone, which scaffolds comprise at least two phases, wherein each phase differs in terms of its chemical content, or structure, kits comprising the same, processes for producing solid aragonite or calcite scaffolds and methods of use thereof.

The invention provides a self-adaptive multifunctional hydrogel system for cardiac microenvironment regulation after myocardial infarction as well as a preparation method and application of the self-adaptive multifunctional hydrogel system, and belongs to the technical field of biomedical materials. The hydrogel system is hydrogel prepared by taking a micromolecular cross-linking agent, a polymer matrix and drug-loaded microspheres as raw materials. The hydrogel can realize staged response drug release in oxidative stress, inflammation and fibrosis microenvironments after myocardial infarction, has good injectability and self-healing property, and has the functions of ROS removal, nitric oxide release and the like. The invention provides a new strategy for tissue repair after myocardial infarction with multi-channel collaboration and accurate pathological response control.

Provided are tissue repair and sealing devices, and methods for the use of tissue repair and sealing devices, for use in both minimally invasive surgical (MIS) procedures and open, non-MIS procedures to rapidly repair tissue fenestrations and create a pressure-resistant, watertight seal in a tissue barrier. Tissue repair and sealing devices disclosed herein comprise an integrated graft and deployable clasp assembly and an applicator assembly having a graft-clasp lock cylinder that is slidably connected to a folded, deployable clasp. The applicator assembly places a graft on a tissue inner surface and a deployable clasp on a tissue outer surface to secure the graft to the tissue inner surface to, thereby, repair a tissue fenestration and create a watertight barrier.

The invention discloses a derivative of a rice bran extract as well as a preparation method and application of the derivative. A C-3 benzoate prodrug system is constructed, the transmembrane absorption efficiency is remarkably improved by optimizing the oil-water partition coefficient, fixed-point slow release of active ingredients is achieved through inflammatory tissue esterase, and high bioavailability and low irritation are both considered. Meanwhile, C-25 fluorine atoms are introduced to serve as biological electron isosteres, and target affinity is enhanced by means of the unique electronic effect and polar hydrophobicity of the C-25 fluorine atoms; spatial conformation is locked through side chain saturation, so that the compound shows anti-inflammatory and tissue repair activity which is obviously superior to that of natural cycloartanol under the same dosage.

The application provides an active exosome stent coating, a vascular stent and a preparation method and application, and belongs to the technical field of biomedical intervention instruments. The active exosome stent coating of the application adopts stem cell-derived myocardial cell active exosomes which are strengthened by active components of traditional Chinese medicine. Compared with commercial myocardial cells, the stem cell-derived myocardial cells have more significant effects of promoting blood vessels and tissue repair and regeneration, and stronger cytokine secretion capacity. The preparation method of the active exosome stent coating of the application adjusts the introduction sequence and premixing mode of active components such as exosomes and drugs, and enhances the homogeneity and stability. The vascular stent prepared by using the active exosome stent coating of the application has reasonable sequential release kinetics design, can realize precise targeted regulation on human coronary artery smooth muscle cells (HCASMC) in vitro, does not affect the repair potential of endothelial cells, and solves the contradiction between traditional stents in terms of "anti-proliferation and promotion of healing".

Improved allografts having controlled and consistent properties and useful for soft tissue repair, including breast reconstruction, and other surgical procedures, are disclosed. In one embodiment, an improved acellular dermal matrix (ADM) is produced from a skin sample, where the ADM consists essentially of a single dermal tissue type (e.g., papillary or reticular) and comprises a collagen matrix having substantially uniform density and porosity. In another embodiment, two ADMs are produced from the same dermal tissue type (e.g., papillary or reticular) of the same skin sample, each of which consists essentially of reticular dermis, and comprises a collagen matrix having substantially uniform density and porosity. All such improved ADMs are suitable for use in breast reconstruction and other tissue repair and modification surgery procedures.

The present invention provides a composition for restoration of tissue including a biodegradable polymeric copolymer which is obtained by polymerizing a hydrophobic biodegradable polymer and a hydrophilic biodegradable polymer. The composition of the present invention has excellent tissue restoration effects because of high collagen production rate without occurrence of nodules even when injected directly into the dermis, unlike the conventional composition for restoration of tissue.