440 results about "Cell sheet" patented technology

The invention relates to the technical field of bioinformatics, and provides a visual analysis method and system for a rice multi-tissue single cell expression profile. The method comprises the following steps: comparing sequencing data of an original single cell transcriptome of a rice tissue to obtain a standardized transcriptome data set; performing batch effect correction and integration on the standardized transcriptome data set to obtain a whole plant expression matrix; performing cell type annotation on the whole plant expression matrix to obtain a cell type annotation system; carrying out visual dimension reduction processing on the whole plant expression matrix fused with the cell type annotation system, and carrying out co-expression network construction to obtain a modular tissue correlation analysis model; and establishing an interaction end based on the module organization correlation analysis model, and realizing data visualization analysis through the interaction end. The invention provides a one-stop analysis platform for rice cell heterogeneity research, functional gene mining and molecular breeding.

The invention provides an antibody for resisting CD19 on the surface of a porcine B cell or an antigen binding fragment thereof as well as a composition and application thereof. Specifically, the invention provides an anti-porcine B cell surface CD19 antibody or an antigen binding fragment thereof, which can effectively bind to CD19 on the porcine B cell surface. The invention also provides an antibody drug conjugate containing the antibody or the antigen binding fragment thereof, and the antibody drug conjugate can be applied to screening of porcine B cells and marking of CD19 antigens on the surfaces of the porcine B cells, and has a good application prospect.

The invention provides a back contact battery assembly and a photovoltaic system, a back contact battery can comprise a plurality of battery strings and a bus bar, a first welding strip comprises a first body section connected with a first battery piece and a first extension section which extends to a second battery piece and is at least partially overlapped with the bus bar, the second welding strip comprises a second body section and a second extension section. And in the thickness direction of the back contact battery assembly, the first extension section and the second extension section do not have an overlapped part. The bus bar is provided with a protruding section protruding towards the side away from the second battery piece at the second extension section, and the bus bar is provided with a recessed section recessed towards the side of the second battery piece at the first extension section. For at least part of the cross section of the back contact battery assembly at the bus bar, the height difference between the highest point of the first extension section and the highest point of the adjacent convex section is smaller than the thickness of the first welding strip. In this way, the hidden crack risk in the laminating process can be reduced.

The invention relates to the technical field of photovoltaic cells, in particular to a preparation method of a back contact cell string and a photovoltaic module. The preparation method of the back contact cell string comprises the following steps: a glue setting step: setting insulation glue at a position, close to a first solder strip to be set, of a cell piece and on a second thin grid line, setting insulation glue at a position, close to a second solder strip to be set, of the cell piece and on the first thin grid line, and setting pre-solidified glue at the first solder strip to be set and the second solder strip to be set; the glue is cured, specifically, the glue is cured so that the insulation glue can be cured to the preset hardness, and the pre-cured glue is not cured or not completely cured; and a welding strip laying step: laying the first welding strip and the second welding strip. The photovoltaic module comprises the cell string formed by adopting the preparation method of the back contact cell string. The invention provides a preparation method of a back contact cell string and a photovoltaic module. The technical problem that in the prior art, the preparation process of a back contact cell is long is solved.

The invention belongs to the technical field of preparation of polypeptides, and particularly relates to a self-assembled polypeptide capable of responding to alkaline phosphatase and degrading Neuropilin-1 as well as a preparation method and application of the self-assembled polypeptide. The self-assembled polypeptide (formula I) provided by the invention has a self-assembly starting unit 'GNNQQNY (SEQ ID NO: 1)', can be self-assembled in vitro, and can form nanofibers after responding to alkaline phosphatase, so that not only can the critical self-assembly concentration of the polypeptide be reduced, but also the binding capacity with target protein can be improved. The material can degrade NRP1 on the surfaces of tumor cells and Treg cells, and has huge potential in promoting immunotherapy of tumors.

Provided is a method for screening myocardial therapeutic targets for ischemic heart failure by using single-cell sequencing, which method comprises the following steps: S1, sample preparation; S2, construction of a single-cell expression matrix; S3, cell quality control; S4, cell type annotation; S5, cell communication analysis; and S6, co-expression network analysis. The provided method for screening myocardial therapeutic targets for ischemic heart failure by using single-cell sequencing comprises performing single-cell sequencing on hearts of healthy mice and IHF mice, screening for cell types with significant differences in cardiac transcriptional profiles of the healthy mice and IHF mice, then exploring interaction characteristics of various types of cells in malignant fibrotic IHF hearts, revealing potential regulatory modules and pathways related to malignant myocardial fibrosis in single-cell expression data of IHF hearts, and performing screening to obtain Pdgfb and Tnfsf12 genes which can be used as therapeutic targets for treating myocardial fibrosis in ischemic heart failure.

The invention discloses an RNA (Ribonucleic Acid) binding protein enrichment analysis method based on single cell expression profile data, which comprises the following steps of: writing an interactive online Web application program by using Shiny, carrying a data visualization tool, and using an RBP (Reactive Binding Protein)-gene database as a database; the method is specially designed for single cell data, different single cell data can be integrated for enrichment analysis, and expression profiles and RBP dynamic changes in single cells can be effectively captured and analyzed; rBP enrichment analysis is customized for single cell data, a single group and multiple groups of single cell expression data are preprocessed, including normalization processing, and a statistical model is used to evaluate enrichment significance at each time point, so that the analysis accuracy and biological correlation are improved; a dynamic visualization tool is provided, the dynamic visualization tool comprises a customizable network diagram, an interactive bar diagram and detailed table display, and a user can adjust a plurality of parameters of a view according to needs and directly download images and data from an interface.

A photovoltaic module and a photovoltaic system. The photovoltaic module includes a panel, a back plate, a cell sheet layer, and a first encapsulation adhesive film layer, wherein the back plate and the panel are stacked, the cell sheet layer is located between the panel and the back plate, the first encapsulation adhesive film layer is located between the cell sheet layer and the panel, a color layer is provided between the panel and the first encapsulation adhesive film layer, the color layer is a light-transmitting layer, and a relief structure is provided on the side of the panel facing away from the first encapsulation adhesive film layer. The relief structure can reduce the reflection of sunlight on the panel, thereby increasing the incident amount of sunlight and increasing the power generation of solar energy.

Methods for preparing a personalized cancer vaccine and a method to train a machine learning HLA-peptide presentation prediction model. Further wherein, a method of making a HLA class II tetramer or multimer comprising an epitope, the method comprising contacting a purified soluble HLA-DM loaded with a peptide epitope with a HLA class II tetramer or multimer, thereby forming a HLA class II tetramer or multimer loaded with the peptide epitope, is disclosed.

The present invention relates to the technical field of stem cell detection, and particularly relates to a determination method for purity and population doubling time of human umbilical cord mesenchymal stem cells (hUC-MSCs). The cultured hUC-MSCs are mixed with the buffer solution to obtain cell suspension; the cell suspension is mixed and incubated with a fluorescein-labeled antibody to obtain an incubated substance; and the incubated substance is centrifuged, and the precipitate obtained is cleaned and then mixed with the buffer solution to obtain a substance to be tested. The present invention analyzes cell surface markers and cell cycles by using a flow cytometer and monoclonal antibodies labeled with different fluoresceins of hUC-MSCs as well as PI staining, and further defines a determination method for purity and population doubling time of hUC-MSCs.

The present disclosure relates to a mammalian cell which is modified to express on the surface of its membrane a binding domain which binds to a target molecule. The disclosure also relates to protein constructs and nucleic acids for producing such modified mammalian cells, and to methods for using the mammalian cells to deliver therapeutic agents to target cells or tissues in vivo.

Provided are a method for inducing differentiation of pluripotent stem cells into CD34+ hematopoietic stem / progenitor cells and a culture medium composition thereof. Provided are a method for inducing differentiation of pluripotent stem cells into CD34+ hematopoietic stem / progenitor cells or NK cells and a culture medium composition thereof. The NK cells obtained using the provided culture method have high purity and good in vitro expansion effect; the yield of iNK cells is high, and a single iPSC can differentiate into approximately 2,000 NK cells; and the obtained iNK cells highly express CD16 (over 70%). As a result, the problem of low CD16 expression in iNK cells in the prior art, which requires genetic modification methods to solve, has been addressed.

The invention provides an anti-PD-1 antibody, a CAR-T cell, and a preparation method and application thereof. The anti-PD-1 antibody comprises LCDR-1-3 as shown in SEQ ID NO: 1-3 and HCDR-1-3 as shown in SEQ ID NO: 4-6, respectively. The CAR-T cell expresses a novel element for efficiently blocking the PD-1, and the element is a single-chain antibody for targeting the PD-1 in a cell membrane anchoring manner. And the chimeric antigen receptor and the cell membrane anchored anti-PD-1 scFv are connected by a 2A cleavage protein. The membrane anchor type anti-PD-1 scFv expressed by the CAR-T cell can almost completely block the expression of PD-1 on the surface of a T cell membrane, and further block a signal channel combined with PD-1 / PD-L1, so that the capability of T cell depletion caused by antagonism tumor of the CAR-T cell is enhanced, and the purpose of enhancing the anti-tumor effect of the CAR-T cell is achieved.

The application provides a photovoltaic superfine active solder wire, a gridless TOPCon cell and an assembly and a preparation method, wherein the active solder of the photovoltaic superfine active solder wire comprises a first component and a matrix selected from specific substances; the first component can increase the wetting performance of the matrix solder, strengthen the beta-Sn phase / In phase / Zn phase grains in the solder matrix, improve the welding strength, reduce the risk of grid breakage of the assembly, and enhance the reliability of the assembly; the foregoing photovoltaic superfine active solder wire is used to replace the silver grid line in the prior art, the silver grid line does not need to be printed and sintered in the cell manufacturing process, the slurry and the printing and sintering process and equipment thereof are omitted, in addition, the transmission resistance and the contact resistance on the current output path of the cell after welding are small, and the electrical performance of the cell can be improved.

The invention provides a mother battery piece, a battery piece, a preparation method of the mother battery piece and a photovoltaic module. The battery piece comprises a first semiconductor substrate; the first doped semiconductor layer is arranged in the central region; the isolation groove is formed in the first isolation area and surrounds the central area; wherein the side surface comprises a first sub-surface, and the first sub-surface is connected with the groove wall of the isolation groove; the tunneling passivation contact structure is at least arranged on the second surface; the first passivation layer at least covers the first doped semiconductor layer, the isolation groove and the first sub-surface; wherein the first sub-surface and the second surface are both provided with a plurality of square tower footing structures, the difference between the side length of the largest square tower footing structure on the first sub-surface and the side length of the largest square tower footing structure on the second surface is a first numerical value, and the first numerical value is smaller than or equal to 15 microns. The battery efficiency can be improved, and the manufacturing cost can be reduced.

The invention discloses a silicon wafer and a preparation method therefor, a cell sheet, a cell slice, a cell string, and a photovoltaic module. The monocrystalline silicon wafer comprises a silicon wafer main body and an extension edge that extends outwards from an edge of the silicon wafer main body, the silicon wafer main body is a right-angled square slice or a rounded square slice, the extension edge is a ribbon-shaped structure parallel to the edge of the silicon wafer main body, and the extension edge is used to overlap below the adjacent monocrystalline silicon wafer during welding. By making the extension edge overlap below the adjacent cell sheet or cell slice, a sheet gap is reduced, an increase in an arrangement density of the cell sheets or cell slices is facilitated, and an efficiency of a photovoltaic module is increased. Since the adjacent cell sheet or cell slice shields the extension edge rather than the silicon wafer main body, the area of the cell sheet or cell slice involved in power generation is increased, and an increase in the power of the photovoltaic module is facilitated.

The invention provides an application of M1 as an affinity of human source PD-L1 and / or mouse source PD-L1, and relates to the technical field of biological medicine. The invention proves that M1 can be used as a PD-L1 affinity for human / mouse cross reaction, and has low nanomole affinity for two targets. In cell-based function detection, M1 has similar affinity with mPDL1 and hPD-L1 expressed on the surface of a cell, and signal transduction is inhibited through a PD-L1: PD1 axis at a low micromolar concentration. The M1 disclosed by the invention can be used as a non-antibody treatment method to damage a PD-L1: PD1 signal channel, and the M1 shows high tumor permeability compared with an antibody, and particularly has a remarkable advantage in tumors (such as pancreatic tumors) with high fiber hyperplasia; the M1 shows selective uptake in the tumor for expressing the PD-L1.

The invention relates to a method for rapidly preparing a cell scanning electron microscope sample through cell surface metal deposition. The method comprises immobilizing a cell sample with an immobilizing agent; incubating in a catalyst solution; placing in a metal deposition solution for cell metal deposition; the cell sample is obtained. The prepared cell sample can be directly used for scanning electron microscope imaging after being dried. According to the method, the micro-nano structure on the surface of the cell is directly mechanically enhanced in the solution through electroless metal deposition, the cell sample is endowed with electrical conductivity, additional chemical modification on the cell is not needed, gradient dehydration on the cell is not needed, and metal spraying treatment on the cell is not needed; and cell scanning electron microscope sample preparation and imaging can be conveniently and quickly carried out.

The invention provides a novel back contact photovoltaic cell film color sorting method, and relates to the technical field of back contact photovoltaic cell film color sorting.The novel back contact photovoltaic cell film color sorting method comprises a test table top, a back contact photovoltaic cell is arranged on the upper surface of the test table top, and a first test positioning device is arranged at the upper left corner of the test table top; the upper right corner of the test table board is provided with a second test point, the lower left corner of the test table board is provided with a third test point, the lower right corner of the test table board is provided with a fourth test point, and the center of the test table board is provided with a fifth test point. According to the method, the lightness value (L), the red-green chroma value (a) and the yellow-green chroma value (b) of the battery piece are tested by the spectrum luminosity tester through the Lab color model and are compared with the film color standard value to calculate the color difference value, whether the battery piece has color difference or not can be accurately determined through the HSV color model and the lab color model, operation is easy, production line application is convenient, the situation that color difference pieces are mixed into bulk goods can be eradicated, and the production efficiency is improved. And the product quality is improved.

The invention relates to a welding method of a solar battery, which comprises the following steps of: providing a battery piece, and arranging a grid line on the side surface of the battery piece; forming an organic solderability preservative film on the side surface of the battery piece, and enabling the organic solderability preservative film to cover the outer surface of the grid line; completely removing the organic solderability preservative film covering the part, needing to be welded with the welding wire, of the grid line, so that the part, needing to be welded with the welding wire, of the grid line is exposed; wherein in the process of removing the organic solderability preservative film at the position, where the welding wire needs to be welded, on the grid line, the temperature of the position, except for the welding wire, on the battery piece is made to be smaller than or equal to 200 DEG C; and the exposed parts on the grid lines are welded and connected with the welding wires. On one hand, the organic solderability preservative film covers the outer surface of the grid line, an anti-oxidation effect can be achieved, and compared with a tin plating process, the production cost is low; and on the other hand, the temperature of the parts except for the welding wire on the battery piece is controlled to be less than or equal to 200 DEG C, and the temperature is relatively low, so that the influence on the performance of an amorphous silicon layer, a silicon substrate and the like of the battery piece can be avoided.

The invention relates to an antibody or an antigen fragment of a targeted human CD7 and application of the antibody or the antigen fragment. The antibody and the CD7-CAR based on the antibody fragment have extremely strong affinity with a CD7 antigen molecule, and a blocking molecule containing the antibody can almost completely block the expression of the CD7 molecule on the cell surface without influencing the normal amplification of T cells, so that the self-killing of the CD7-CAR-T cells can be effectively avoided. The CD7-CAR-T cell constructed on the basis of the single-domain antibody of the targeted human source CD7 has a strong killing effect on a target cell.

The invention provides a method for detecting cell surface RNA glycosylation based on flow cytometry, and belongs to the field of RNA glycosylation detection. The method comprises the following steps: carrying out azido sugar labeling on RNA on the surface of a cell by utilizing a metabolic pathway, coupling an azido group with a DBCO-fluorescent probe based on a click chemistry reaction, and detecting a fluorescent signal by utilizing flow cytometry, so as to realize efficient and sensitive detection on cell RNA glycosylation. The method is easy and convenient to operate and high in flux, has good repeatability and quantification capacity, provides powerful technical support for functional research of cell surface RNA glycosylation, is suitable for researching the effect of cell surface RNA glycosylation in immune regulation and control, and provides a new technical means for further revealing a cell communication mechanism related to RNA glycosylation.

The utility model discloses a full-automatic liquid-based thin-layer cell pelleter which is more convenient compared with a traditional sealing mode such as manual pressing and the like by adsorbing and fixing an opening and closing plate and an opening end through magnetic force of an electromagnet for the whole working environment, and meanwhile, an occlusal pad is additionally arranged to increase the sealing performance of an arc-shaped side surface, so that the production efficiency is improved. The stability of the temperature and the humidity of a working environment is ensured, the possibility that a sample is evaporated and dried is reduced, so that the uniformity of the sample is kept, the uniform mixing efficiency is improved by additionally arranging the uniform mixing mechanism, meanwhile, when the sample is output to the glass slide, a traditional needle center liquid outlet mode is changed into a circular ring liquid outlet mode, the uniform distribution of cells on the glass slide is ensured, and the cell quality is improved. The cell enrichment degree is ensured.

The present invention relates to a method for identifying one or more caudal midbrain (cVM) progenitor cells by measuring the presence and / or amount of adenomatous colonic polyposis down-regulating 1 (APCDD1) expressed by one or more cells. The invention also provides a method for enriching cVM cells, application of the cells in disease treatment, application of APCDD1 as a biomarker and a kit.

The present disclosure relates to methods of treating, preventing, or modulating diabetes in a patient in need thereof using a device for transplanting cells into a host body, specifically a method of treating diabetes in a patient in need thereof, comprising: implanting a device in the patient, wherein the device comprises: a porous scaffold comprising an immunologically compatible polymer mesh forming the walls of at least one chamber, an opening at either or both of a proximal end and a distal end of the chamber, at least one removable, non-porous plug configured to be positioned within the lumen of the at least one chamber, maintaining the device in the patient's body until the device is infiltrated with vascular and connective tissues; and infusing the chamber with cells, wherein at least some of the cells express insulin

The invention provides an antibody for resisting CD19 on the surface of a porcine B cell or an antigen binding fragment thereof as well as a composition and application thereof. Specifically, the invention provides an anti-porcine B cell surface CD19 antibody or an antigen binding fragment thereof, which can effectively bind to CD19 on the porcine B cell surface. The invention also provides an antibody drug conjugate containing the antibody or the antigen binding fragment thereof, and the antibody drug conjugate can be applied to screening of porcine B cells and marking of CD19 antigens on the surfaces of the porcine B cells, and has a good application prospect.

The application provides a nanobody targeting human transmembrane activator and calcium modulator and cyclophilin interactor (TACI) and an application thereof, and the antibody is derived from a llama heavy chain antibody variable region. The anti-TACI single-domain antibody NB38 can specifically bind to human TACI with high affinity, can recognize a recombinant TACI protein, can also recognize a TACI with a natural conformation on a cell surface, and can partially block a BAFF / APRIL signal pathway. Based on the nanobody, the application constructs a fusion protein, a chimeric antigen receptor, an effector cell expressing the chimeric antigen receptor, and a double-specific cell linker and other genetically engineered forms. The nanobody can be further extended into a drug coupling form. The product of the application can be used for mediating directional recognition and killing of TACI positive cells, and can be used as a supplement and expansion of BCMA targeted therapy, and provides a new candidate technical scheme for targeted therapy of multiple myeloma and other TACI related diseases.

The present invention provides a device capable of stably conveying a tray of photovoltaic cell sheets, comprising a feeding rack and an adjusting rack, wherein the adjusting rack is arranged on one side of the feeding rack, an adjusting conveying module for conveying the tray is provided on the adjusting rack, adjustment mechanisms are provided on both sides and the adjusting racks at both ends of the adjusting conveying module, a feeding mechanism is provided below the feeding rack, a feeding module is provided at one end of the feeding rack, the feeding module is used to transfer the photovoltaic cell sheets to the tray on the adjusting conveying module, the adjusting conveying module conveys the tray to the feeding mechanism, so that the tray can remain stable during the movement, thereby ensuring that the position of the cell sheets during the conveying of the tray is relatively accurate, and ensuring that the cell sheets will not be accidentally displaced during the movement of the tray.

An assay for autologous primary immune cells is described, in which individual blood cells can be functionally screened simultaneously for individual antigens of interest, such as T-cell epitopes, without the need for HLA haplotype-specific reagents. An oligonucleotide-labeled hash-tracking system, followed by deconvolution via single-cell sequencing, correlates antigen reactivity with individual T cells.

The invention discloses a method for bifluorescence quantitative detection of epithelial cells of a lacrimal gland organ and application. The method comprises the following steps: providing a mouse lacrimal gland organ; a recombinant adeno-associated virus combination is added to the organoid, the combination comprising: a first AAV vector comprising a promoter specifically driving the expression of alveolar epithelial cells and a first fluorescent protein coding sequence; the second AAV vector contains a promoter for specifically driving expression of myoepithelial cells and a second fluorescent protein coding sequence; according to the method, the two cells can be visually distinguished, and by calculating the fluorescence area or the number of the cells, the alveolar / muscular epithelial cell proportion is established as a damage index, so that high-throughput drug screening is realized.