609 results about "Cell sheet" patented technology

The invention relates to the technical field of bioinformatics, and provides a visual analysis method and system for a rice multi-tissue single cell expression profile. The method comprises the following steps: comparing sequencing data of an original single cell transcriptome of a rice tissue to obtain a standardized transcriptome data set; performing batch effect correction and integration on the standardized transcriptome data set to obtain a whole plant expression matrix; performing cell type annotation on the whole plant expression matrix to obtain a cell type annotation system; carrying out visual dimension reduction processing on the whole plant expression matrix fused with the cell type annotation system, and carrying out co-expression network construction to obtain a modular tissue correlation analysis model; and establishing an interaction end based on the module organization correlation analysis model, and realizing data visualization analysis through the interaction end. The invention provides a one-stop analysis platform for rice cell heterogeneity research, functional gene mining and molecular breeding.

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

The invention provides an antibody for resisting CD19 on the surface of a porcine B cell or an antigen binding fragment thereof as well as a composition and application thereof. Specifically, the invention provides an anti-porcine B cell surface CD19 antibody or an antigen binding fragment thereof, which can effectively bind to CD19 on the porcine B cell surface. The invention also provides an antibody drug conjugate containing the antibody or the antigen binding fragment thereof, and the antibody drug conjugate can be applied to screening of porcine B cells and marking of CD19 antigens on the surfaces of the porcine B cells, and has a good application prospect.

The present invention discloses a combination of different nucleic acid aptamers with 291 known targets. By performing sequencing analysis on the nucleic acid aptamer sequences of this combination through single-cell high-throughput sequencing technology, a cell atlas targeting mononuclear cells derived from the peripheral blood of patients with cholangiocarcinoma can be constructed. The nucleic acid aptamers covered by this combination have high specific affinity for their molecular targets. After extending adapters commercialized for sequencing platforms at both ends of the nucleic acid aptamer sequences respectively, by performing high-throughput sequencing on the nucleic acid aptamer sequences with sequencing adapters bound to the surface of mononuclear cells, high-throughput analysis of biomarkers on the surface of mononuclear cells derived from the peripheral blood of patients with cholangiocarcinoma can be achieved, thereby constructing a cell atlas of mononuclear cells derived from the peripheral blood of cholangiocarcinoma.

The invention relates to an aquaporin bionic nanofiltration membrane and a preparation method and application thereof.The aquaporin bionic nanofiltration membrane is composed of a cell sheet rich in aquaporin and a porous base membrane, and the cell sheet rich in aquaporin serves as a functional unit; through a vacuum filtration method, layer-by-layer assembly and cross-linking curing, the bonding firmness in the active layer is improved. The preparation method disclosed by the invention is simple in operation process, the embedding amount of the aquaporin in the nanofiltration membrane is increased, and the biological activity of the aquaporin is ensured. The aquaporin bionic nanofiltration membrane disclosed by the invention is high in stability and excellent in permeation selectivity, and has a wide application prospect in the field of membrane separation.

The invention provides a back contact battery assembly and a photovoltaic system, a back contact battery can comprise a plurality of battery strings and a bus bar, a first welding strip comprises a first body section connected with a first battery piece and a first extension section which extends to a second battery piece and is at least partially overlapped with the bus bar, the second welding strip comprises a second body section and a second extension section. And in the thickness direction of the back contact battery assembly, the first extension section and the second extension section do not have an overlapped part. The bus bar is provided with a protruding section protruding towards the side away from the second battery piece at the second extension section, and the bus bar is provided with a recessed section recessed towards the side of the second battery piece at the first extension section. For at least part of the cross section of the back contact battery assembly at the bus bar, the height difference between the highest point of the first extension section and the highest point of the adjacent convex section is smaller than the thickness of the first welding strip. In this way, the hidden crack risk in the laminating process can be reduced.

The invention relates to the technical field of photovoltaic cells, in particular to a preparation method of a back contact cell string and a photovoltaic module. The preparation method of the back contact cell string comprises the following steps: a glue setting step: setting insulation glue at a position, close to a first solder strip to be set, of a cell piece and on a second thin grid line, setting insulation glue at a position, close to a second solder strip to be set, of the cell piece and on the first thin grid line, and setting pre-solidified glue at the first solder strip to be set and the second solder strip to be set; the glue is cured, specifically, the glue is cured so that the insulation glue can be cured to the preset hardness, and the pre-cured glue is not cured or not completely cured; and a welding strip laying step: laying the first welding strip and the second welding strip. The photovoltaic module comprises the cell string formed by adopting the preparation method of the back contact cell string. The invention provides a preparation method of a back contact cell string and a photovoltaic module. The technical problem that in the prior art, the preparation process of a back contact cell is long is solved.

The invention belongs to the technical field of preparation of polypeptides, and particularly relates to a self-assembled polypeptide capable of responding to alkaline phosphatase and degrading Neuropilin-1 as well as a preparation method and application of the self-assembled polypeptide. The self-assembled polypeptide (formula I) provided by the invention has a self-assembly starting unit 'GNNQQNY (SEQ ID NO: 1)', can be self-assembled in vitro, and can form nanofibers after responding to alkaline phosphatase, so that not only can the critical self-assembly concentration of the polypeptide be reduced, but also the binding capacity with target protein can be improved. The material can degrade NRP1 on the surfaces of tumor cells and Treg cells, and has huge potential in promoting immunotherapy of tumors.

The invention relates to the technical field of solar cells, and particularly discloses a method for improving conductive contact of a micro-area of an N-type cell, which comprises the following steps of: (1) performing first micro-area conductive treatment on one side of a cell grid line of the N-type cell: scanning a first side edge of the cell grid line through a first pulse laser beam, and applying a first reverse bias voltage to the cell at the same time; and (2) performing second micro-area conductive treatment on the other side of the cell grid line of the N-type cell: scanning a second side edge, opposite to the first side edge, of the cell grid line through a second pulse laser beam, and applying second reverse bias voltage to the cell at the same time. According to the method, the pulse laser beams with more concentrated energy are used for carrying out single-side scanning on the two side edges of the grid line of the battery piece respectively, and precise treatment on the metal electrode on the basis of not damaging the passivation layer of the battery can be realized, so that the contact performance of the battery is optimized, the contact resistance of the battery is greatly reduced, and the performance of the battery piece is improved.

The invention relates to a preparation method of a 3D printing high-density cell tubular vascular graft. The preparation method comprises the following steps: sucking a supernatant obtained by centrifuging a cell suspension away and only remaining bottom pure cells; the preparation method comprises the following steps: uniformly stirring and mixing OMA, pig gelatin and an LAP photoinitiator in a Hank's solution to form a hydrogel precursor solution; extruding the pure cells and the hydrogel precursor solution from a three-axis nozzle by using printing equipment, and immersing the pure cells and the hydrogel precursor solution into a calcium chloride solution; lyase is added to degrade the hydrogel precursor solution, the hydrogel precursor solution is completely degraded, and an independent single-layer pipeline structure with only a remaining pure cell layer is obtained. The invention provides a method for manufacturing high-density pure-cell lumen constructs to mimic native blood vessels by using a self-made microfluidic coaxial 3D bioprinting device. And after the support hydrogel on the inner layer and the outer layer is removed, a single-layer tubular structure of high-density MOVAs only limited to pure cells is obtained. The cells in the pure cell vascular constructs exhibit good cell viability and cell function.

Provided is a method for screening myocardial therapeutic targets for ischemic heart failure by using single-cell sequencing, which method comprises the following steps: S1, sample preparation; S2, construction of a single-cell expression matrix; S3, cell quality control; S4, cell type annotation; S5, cell communication analysis; and S6, co-expression network analysis. The provided method for screening myocardial therapeutic targets for ischemic heart failure by using single-cell sequencing comprises performing single-cell sequencing on hearts of healthy mice and IHF mice, screening for cell types with significant differences in cardiac transcriptional profiles of the healthy mice and IHF mice, then exploring interaction characteristics of various types of cells in malignant fibrotic IHF hearts, revealing potential regulatory modules and pathways related to malignant myocardial fibrosis in single-cell expression data of IHF hearts, and performing screening to obtain Pdgfb and Tnfsf12 genes which can be used as therapeutic targets for treating myocardial fibrosis in ischemic heart failure.

Provided is an anti-CLL1 antibody and an application thereof. The variable region of the anti-CLL1 antibody includes the CDRs of SEQ ID NO: 1 to 6, SEQ ID NO: 5, and SEQ ID NO: 7 to 11 or SEQ ID NO: 12 to 17. The anti-CLL1 antibody of the present application has significant binding ability to both free and cell surface CLL1. After humanization, the affinity of the antibody to CLL1 is further improved, and it has important application prospect in the clinical diagnosis and / or treatment of tumors.

The invention discloses an RNA (Ribonucleic Acid) binding protein enrichment analysis method based on single cell expression profile data, which comprises the following steps of: writing an interactive online Web application program by using Shiny, carrying a data visualization tool, and using an RBP (Reactive Binding Protein)-gene database as a database; the method is specially designed for single cell data, different single cell data can be integrated for enrichment analysis, and expression profiles and RBP dynamic changes in single cells can be effectively captured and analyzed; rBP enrichment analysis is customized for single cell data, a single group and multiple groups of single cell expression data are preprocessed, including normalization processing, and a statistical model is used to evaluate enrichment significance at each time point, so that the analysis accuracy and biological correlation are improved; a dynamic visualization tool is provided, the dynamic visualization tool comprises a customizable network diagram, an interactive bar diagram and detailed table display, and a user can adjust a plurality of parameters of a view according to needs and directly download images and data from an interface.

The invention discloses a recombinant strain for biosynthesis of gamma-aminobutyric acid as well as a construction method and application of the recombinant strain, and belongs to the technical field of gene engineering. In order to solve the technical problems that GAD (gamma-aminobutyric acid) is seriously limited to be synthesized by corynebacterium glutamicum by adopting a cell surface display technology because the growth of corynebacterium glutamicum and the production of GABA (gamma-aminobutyric acid) have difference on pH (potential of hydrogen) requirements and L-glutamic acid has a feedback inhibition effect on the synthesis process of the L-glutamic acid, the invention adopts a cell surface display technology to display GAD on the outer surface of a cell. Therefore, the GAD not only can exert higher activity in a more suitable meta-acidic environment, but also can directly convert a precursor L-glutamic acid continuously secreted to extracellular into extracellular GABA, and extracellular transport of the GABA does not need to be carried out any more. Furthermore, in order to more efficiently and simultaneously transform intracellular and extracellular L-glutamic acid, GAD is expressed in cells on the basis of displaying GAD on the surfaces of the cells, so that the L-glutamic acid is simultaneously transformed in the cells and extracellular, and synthesis of GABA (gamma-aminobutyric acid) is accelerated.

A photovoltaic module and a photovoltaic system. The photovoltaic module includes a panel, a back plate, a cell sheet layer, and a first encapsulation adhesive film layer, wherein the back plate and the panel are stacked, the cell sheet layer is located between the panel and the back plate, the first encapsulation adhesive film layer is located between the cell sheet layer and the panel, a color layer is provided between the panel and the first encapsulation adhesive film layer, the color layer is a light-transmitting layer, and a relief structure is provided on the side of the panel facing away from the first encapsulation adhesive film layer. The relief structure can reduce the reflection of sunlight on the panel, thereby increasing the incident amount of sunlight and increasing the power generation of solar energy.

The photovoltaic cell sheet includes: a cell sheet body having a first end and a second end opposite to each other in a width direction of the cell sheet body, wherein the first end of the cell sheet body is provided with a plurality of sawtooth spacing along a length direction of the cell sheet body; a first main gate arranged at one side of front and back sides of the plurality of sawtooth and serving as a first electrode; and a second main gate arranged at another side of the front and back sides of the cell sheet body and serving as a second electrode, wherein the second main gate is adjacent to the second end of the cell sheet body.

Disclosed are fusion proteins (and homodimers and heterodimers thereof). Fusion proteins can bind to a protein of interest and to an internalizing receptor (transferrin receptor) on a cell surface. Once bound, the protein of interest can be internalized and / or degraded inside a cell.

Methods for preparing a personalized cancer vaccine and a method to train a machine learning HLA-peptide presentation prediction model. Further wherein, a method of making a HLA class II tetramer or multimer comprising an epitope, the method comprising contacting a purified soluble HLA-DM loaded with a peptide epitope with a HLA class II tetramer or multimer, thereby forming a HLA class II tetramer or multimer loaded with the peptide epitope, is disclosed.

The invention discloses a method for preparing bovine MSTN gene editing cells by using CRISPR / Cas12i, and belongs to the technical field of biology. According to the invention, an sgRNA expression vector aiming at the bovine MSTN gene is constructed, and a CRISPR / Cas12i system is utilized to carry out precise gene editing on a specific target area of a first exon of the bovine primary fibroblast MSTN gene, so that mRNA of the MSTN gene cannot be normally transcribed, and MSTN gene deleted cells are obtained. Compared with other gene editing tools, the Cas12i system shows higher specificity and editing efficiency (improved by 3%-5%), and the off-target effect risk is effectively reduced. The prepared MSTN gene deleted bovine recombinant cell line is stable and efficient in editing efficiency, the cell expression level is not affected, the MSTN gene deleted bovine recombinant cell line can be widely applied to somatic cell cloning and microinjection preparation of double-muscle-hip beef cattle, and a new strategy is provided for beef cattle breeding.

The invention discloses a lightweight optimal clustering selection method for scRNA-seq data. The lightweight optimal clustering selection method comprises the following steps: 1, acquiring and preprocessing an scRNA-seq data matrix; 2, performing layer-by-layer dimensionality reduction on data by using a multi-layer perceptron encoder to obtain a low-dimensional feature matrix; and 3, constructing an undirected cell map, and enhancing and removing cell map noise through an NE network. And 4, reconstructing features by using graph convolution, inputting the low-dimensional feature matrix and the cell graph into a simplified graph convolution module, and updating the reconstructed feature matrix round by round. And 5, carrying out cell clustering on the characteristic matrix after each round of updating by using self-optimization clustering to obtain cell representation. And 6, judging and evaluating the clustering performance of the current round, storing an optimal clustering result, and carrying out feature reconstruction and self-optimization clustering of the next round. And 7, outputting and analyzing final optimal feature information. According to the method, scRNA-seq data can be well processed, low-dimensional embedded features can be extracted, tag prediction is more accurate, the requirement for hardware resources is low, and an efficient method is provided for analyzing biological characteristics of single cell data.

The present invention relates to the technical field of stem cell detection, and particularly relates to a determination method for purity and population doubling time of human umbilical cord mesenchymal stem cells (hUC-MSCs). The cultured hUC-MSCs are mixed with the buffer solution to obtain cell suspension; the cell suspension is mixed and incubated with a fluorescein-labeled antibody to obtain an incubated substance; and the incubated substance is centrifuged, and the precipitate obtained is cleaned and then mixed with the buffer solution to obtain a substance to be tested. The present invention analyzes cell surface markers and cell cycles by using a flow cytometer and monoclonal antibodies labeled with different fluoresceins of hUC-MSCs as well as PI staining, and further defines a determination method for purity and population doubling time of hUC-MSCs.

The present disclosure relates to a mammalian cell which is modified to express on the surface of its membrane a binding domain which binds to a target molecule. The disclosure also relates to protein constructs and nucleic acids for producing such modified mammalian cells, and to methods for using the mammalian cells to deliver therapeutic agents to target cells or tissues in vivo.

The present invention relates in part to nucleic acids encoding proteins, nucleic acids containing non-canonical nucleotides, therapeutics comprising nucleic acids, methods, kits, and devices for inducing cells to express proteins, methods, kits, and devices for transfecting, gene editing, and reprogramming cells, and cells, organisms, and therapeutics produced using these methods, kits, and devices. Methods for inducing cells to express proteins and for reprogramming and gene-editing cells using RNA are disclosed. Methods for producing cells from patient samples, cells produced using these methods, and therapeutics comprising cells produced using these methods are also disclosed.

The invention relates to a method to determine a homology directed repair (HDR) event within a eukaryotic cell, wherein the cell expresses a first isoform of a surface protein, which is different from a second isoform of said surface protein with regard to an amino acid marker. The method comprises the steps of inducing a DNA double strand break, providing a HDR template DNA construct comprising the amino acid marker corresponding to the second isoform of the surface protein and subsequently determining the expression of the first or second isoform of said surface protein on said cell, wherein expression of the second isoform indicates a successful HDR event. The invention also relates to a method for editing a genomic location of interest within a eukaryotic cell, and to a method of selectively depleting or enriching an edited cell in a composition of non-edited and edited cells.

Provided are a method for inducing differentiation of pluripotent stem cells into CD34+ hematopoietic stem / progenitor cells and a culture medium composition thereof. Provided are a method for inducing differentiation of pluripotent stem cells into CD34+ hematopoietic stem / progenitor cells or NK cells and a culture medium composition thereof. The NK cells obtained using the provided culture method have high purity and good in vitro expansion effect; the yield of iNK cells is high, and a single iPSC can differentiate into approximately 2,000 NK cells; and the obtained iNK cells highly express CD16 (over 70%). As a result, the problem of low CD16 expression in iNK cells in the prior art, which requires genetic modification methods to solve, has been addressed.

A monitoring device of biological cells including: a fluidic channel in which a fluid including biological cells is made to flow, including a first and second glass walls placed in parallel and a reflective surface adjoined to the second wall; and a piezoelectric transducer, the piezoelectric transducer emitting acoustic waves creating a force acting on the biological cells, making them flow substantially along a single motion plane. The external interface between the first wall and the exterior of the fluidic channel and the reflective surface are configured to function as a Fabry-Perot interferometer, such that the motion planes sensibly perpendicular to the optical axis of the Fabry-Perot interferometer. The finesse of the interferometer is lower than 10. The monitoring device further includes a detecting unit detecting a fringe pattern stemming from the Fabry-Perot interferometer and an analysing device, analysing the fringe pattern and configured to output data on physical properties of the cells based on the pattern.

The invention provides an anti-PD-1 antibody, a CAR-T cell, and a preparation method and application thereof. The anti-PD-1 antibody comprises LCDR-1-3 as shown in SEQ ID NO: 1-3 and HCDR-1-3 as shown in SEQ ID NO: 4-6, respectively. The CAR-T cell expresses a novel element for efficiently blocking the PD-1, and the element is a single-chain antibody for targeting the PD-1 in a cell membrane anchoring manner. And the chimeric antigen receptor and the cell membrane anchored anti-PD-1 scFv are connected by a 2A cleavage protein. The membrane anchor type anti-PD-1 scFv expressed by the CAR-T cell can almost completely block the expression of PD-1 on the surface of a T cell membrane, and further block a signal channel combined with PD-1 / PD-L1, so that the capability of T cell depletion caused by antagonism tumor of the CAR-T cell is enhanced, and the purpose of enhancing the anti-tumor effect of the CAR-T cell is achieved.

The present invention provides a method for suppressing a target gene involved in the Wnt / β-catenin signaling pathway in target cells expressing a receptor capable of binding an extracellular Wnt ligand and an intracellular Wnt effector. The method comprises providing nanocarriers and binding the nanocarriers to the target cells and / or internalizing of the nanocarriers into the target cells, whereby the target gene is suppressed in the target cells. In each nanocarrier, an antibody specific for the receptor, a siRNA specific for the effector, or a combination thereof may be attached to a nanoparticle. The target cells may be in a subject. The method may further comprise reducing disease burden and / or inducing stabilization of a disease in the subject. Also provided are methods for synthesizing the nanocarriers.

The present invention provides a dual DNA tetrahedron structure, a preparation method and an application thereof. The dual DNA tetrahedron of the present invention includes a first DNA tetrahedron structure, a second DNA tetrahedron structure and a connecting chain. This structure can specifically recognize two cell surface proteins and be labeled by fluorescence; at the same time, due to the steric hindrance effect of the structure, it is difficult for the two proteins to approach each other, affecting the interaction between the two. This structure can exist on the cell surface for a long time, thus having a good cell regulation effect on these two cell membrane surface proteins, specifically reflected in reducing the phosphorylation level of HER2 protein, promoting the expression of apoptosis-related proteins, and ultimately leading to apoptosis of tumor cells and reducing the cell activity of tumor cells.

The application provides a photovoltaic superfine active solder wire, a gridless TOPCon cell and an assembly and a preparation method, wherein the active solder of the photovoltaic superfine active solder wire comprises a first component and a matrix selected from specific substances; the first component can increase the wetting performance of the matrix solder, strengthen the beta-Sn phase / In phase / Zn phase grains in the solder matrix, improve the welding strength, reduce the risk of grid breakage of the assembly, and enhance the reliability of the assembly; the foregoing photovoltaic superfine active solder wire is used to replace the silver grid line in the prior art, the silver grid line does not need to be printed and sintered in the cell manufacturing process, the slurry and the printing and sintering process and equipment thereof are omitted, in addition, the transmission resistance and the contact resistance on the current output path of the cell after welding are small, and the electrical performance of the cell can be improved.