783 results about "Cell sheet" patented technology

The invention discloses a combination of different nucleic acid aptamers with known 291 targets, sequences of the nucleic acid aptamers of the combination are subjected to sequencing analysis through a single-cell high-throughput sequencing technology, and a cell map aiming at peripheral blood-derived mononuclear cells of a biliary tract cancer patient can be constructed. The nucleic acid aptamers covered by the combination have high specific affinity to molecular targets of the nucleic acid aptamers, and after the two ends of the nucleic acid aptamer sequences extend to adapt to joints of a commercial sequencing platform, high-throughput sequencing is carried out on the nucleic acid aptamer sequences which are combined with the surfaces of mononuclear cells and are provided with the sequencing joints; high-throughput analysis of the biomarkers on the surface of the peripheral blood-derived mononuclear cell of a biliary tract cancer patient can be realized, so that the cell map of the peripheral blood-derived mononuclear cell of the biliary tract cancer is constructed.

The invention relates to the technical field of bioinformatics, and provides a visual analysis method and system for a rice multi-tissue single cell expression profile. The method comprises the following steps: comparing sequencing data of an original single cell transcriptome of a rice tissue to obtain a standardized transcriptome data set; performing batch effect correction and integration on the standardized transcriptome data set to obtain a whole plant expression matrix; performing cell type annotation on the whole plant expression matrix to obtain a cell type annotation system; carrying out visual dimension reduction processing on the whole plant expression matrix fused with the cell type annotation system, and carrying out co-expression network construction to obtain a modular tissue correlation analysis model; and establishing an interaction end based on the module organization correlation analysis model, and realizing data visualization analysis through the interaction end. The invention provides a one-stop analysis platform for rice cell heterogeneity research, functional gene mining and molecular breeding.

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

The present disclosure provides BCMA binding molecules that specifically bind to human BCMA, conjugates comprising the BCMA binding molecules, and pharmaceutical compositions comprising the BCMA binding molecules and the conjugates. The disclosure further provides methods of using the BCMA binding molecules to treat cancers that express cell surface BCMA. The disclosure yet further provides recombinant host cells engineered to express the BCMA binding molecules and methods of producing the BCMA binding molecules by culturing the host cells under conditions in which the BCMA binding molecules are expressed.

The photovoltaic module comprises battery pieces and a welding strip, the multiple battery pieces are arranged in the first direction, projections of every two adjacent battery pieces in the second direction have an overlapping area, every two adjacent battery pieces are connected through the welding strip, each battery piece comprises a first surface and a second surface in the second direction, a first main grid is arranged on the first surface, and a second main grid is arranged on the second surface. The first surface is provided with a first main grid, the first main grid comprises a first flow guide part, the second surface is provided with a second main grid, the second main grid comprises a second flow guide part, the projection area of the first flow guide part is smaller than that of the second flow guide part in the second direction, and the projection of the first flow guide part is located in the projection of the welding strip corresponding to the first flow guide part. According to the design, the shading area of the first diversion part is reduced so as to improve the photoelectric conversion efficiency of the photovoltaic module, the second diversion part is arranged on the second surface of the battery piece, the area of the second diversion part is larger than that of the first diversion part, and the possibility of shading loss of the battery piece caused by the second diversion part is reduced while the current collection capacity of the second diversion part is improved.

The invention provides an antibody for resisting CD19 on the surface of a porcine B cell or an antigen binding fragment thereof as well as a composition and application thereof. Specifically, the invention provides an anti-porcine B cell surface CD19 antibody or an antigen binding fragment thereof, which can effectively bind to CD19 on the porcine B cell surface. The invention also provides an antibody drug conjugate containing the antibody or the antigen binding fragment thereof, and the antibody drug conjugate can be applied to screening of porcine B cells and marking of CD19 antigens on the surfaces of the porcine B cells, and has a good application prospect.

The present invention discloses a combination of different nucleic acid aptamers with 291 known targets. By performing sequencing analysis on the nucleic acid aptamer sequences of this combination through single-cell high-throughput sequencing technology, a cell atlas targeting mononuclear cells derived from the peripheral blood of patients with cholangiocarcinoma can be constructed. The nucleic acid aptamers covered by this combination have high specific affinity for their molecular targets. After extending adapters commercialized for sequencing platforms at both ends of the nucleic acid aptamer sequences respectively, by performing high-throughput sequencing on the nucleic acid aptamer sequences with sequencing adapters bound to the surface of mononuclear cells, high-throughput analysis of biomarkers on the surface of mononuclear cells derived from the peripheral blood of patients with cholangiocarcinoma can be achieved, thereby constructing a cell atlas of mononuclear cells derived from the peripheral blood of cholangiocarcinoma.

The invention relates to an aquaporin bionic nanofiltration membrane and a preparation method and application thereof.The aquaporin bionic nanofiltration membrane is composed of a cell sheet rich in aquaporin and a porous base membrane, and the cell sheet rich in aquaporin serves as a functional unit; through a vacuum filtration method, layer-by-layer assembly and cross-linking curing, the bonding firmness in the active layer is improved. The preparation method disclosed by the invention is simple in operation process, the embedding amount of the aquaporin in the nanofiltration membrane is increased, and the biological activity of the aquaporin is ensured. The aquaporin bionic nanofiltration membrane disclosed by the invention is high in stability and excellent in permeation selectivity, and has a wide application prospect in the field of membrane separation.

A bispecific antibody construct comprising a first human binding domain which binds to human CDH19 on the surface of a target cell and a second binding domain which binds to human CD3 on the surface of a T cell. A nucleic acid comprising a polynucleotide encoding the antibody construct, a vector comprising the nucleic acid comprising the polynucleotide and a host cell transformed or transfected with the nucleic acid comprising the polynucleotide or vector. A process for the production of the antibody construct, a medical use of the antibody construct in the treatment of melanoma, and a kit comprising the antibody construct.

The utility model provides a cell sheet, a cell stacking structure and a solar cell assembly, and relates to the photovoltaic field, the cell sheet is characterized in that at least one of the back surface and the front surface of the cell sheet is provided with a protective glue layer, the back surface is provided with a plurality of electrodes, and the protective glue layer is composed of a plurality of protective glue units arranged at intervals; the minimum distance between every two adjacent protection glue units is smaller than the projection width of electrodes on the surfaces of the battery pieces. According to the utility model, when the battery pieces are stacked, the electrode on the back surface of one battery piece is not in direct contact with the front surface of another adjacent battery piece, so that the effect of protecting the surfaces of the battery pieces is achieved. Besides, the protection adhesive layer is fixed on the front surface of the battery piece, and the protection adhesive layer is not easy to fall off or generate position deviation, so that the protection adhesive layer can generate a continuous protection effect on the surface of the battery piece.

The invention provides a back contact battery assembly and a photovoltaic system, a back contact battery can comprise a plurality of battery strings and a bus bar, a first welding strip comprises a first body section connected with a first battery piece and a first extension section which extends to a second battery piece and is at least partially overlapped with the bus bar, the second welding strip comprises a second body section and a second extension section. And in the thickness direction of the back contact battery assembly, the first extension section and the second extension section do not have an overlapped part. The bus bar is provided with a protruding section protruding towards the side away from the second battery piece at the second extension section, and the bus bar is provided with a recessed section recessed towards the side of the second battery piece at the first extension section. For at least part of the cross section of the back contact battery assembly at the bus bar, the height difference between the highest point of the first extension section and the highest point of the adjacent convex section is smaller than the thickness of the first welding strip. In this way, the hidden crack risk in the laminating process can be reduced.

The invention relates to the technical field of photovoltaic cells, in particular to a preparation method of a back contact cell string and a photovoltaic module. The preparation method of the back contact cell string comprises the following steps: a glue setting step: setting insulation glue at a position, close to a first solder strip to be set, of a cell piece and on a second thin grid line, setting insulation glue at a position, close to a second solder strip to be set, of the cell piece and on the first thin grid line, and setting pre-solidified glue at the first solder strip to be set and the second solder strip to be set; the glue is cured, specifically, the glue is cured so that the insulation glue can be cured to the preset hardness, and the pre-cured glue is not cured or not completely cured; and a welding strip laying step: laying the first welding strip and the second welding strip. The photovoltaic module comprises the cell string formed by adopting the preparation method of the back contact cell string. The invention provides a preparation method of a back contact cell string and a photovoltaic module. The technical problem that in the prior art, the preparation process of a back contact cell is long is solved.

The invention belongs to the technical field of preparation of polypeptides, and particularly relates to a self-assembled polypeptide capable of responding to alkaline phosphatase and degrading Neuropilin-1 as well as a preparation method and application of the self-assembled polypeptide. The self-assembled polypeptide (formula I) provided by the invention has a self-assembly starting unit 'GNNQQNY (SEQ ID NO: 1)', can be self-assembled in vitro, and can form nanofibers after responding to alkaline phosphatase, so that not only can the critical self-assembly concentration of the polypeptide be reduced, but also the binding capacity with target protein can be improved. The material can degrade NRP1 on the surfaces of tumor cells and Treg cells, and has huge potential in promoting immunotherapy of tumors.

The invention relates to the technical field of solar cells, and particularly discloses a method for improving conductive contact of a micro-area of an N-type cell, which comprises the following steps of: (1) performing first micro-area conductive treatment on one side of a cell grid line of the N-type cell: scanning a first side edge of the cell grid line through a first pulse laser beam, and applying a first reverse bias voltage to the cell at the same time; and (2) performing second micro-area conductive treatment on the other side of the cell grid line of the N-type cell: scanning a second side edge, opposite to the first side edge, of the cell grid line through a second pulse laser beam, and applying second reverse bias voltage to the cell at the same time. According to the method, the pulse laser beams with more concentrated energy are used for carrying out single-side scanning on the two side edges of the grid line of the battery piece respectively, and precise treatment on the metal electrode on the basis of not damaging the passivation layer of the battery can be realized, so that the contact performance of the battery is optimized, the contact resistance of the battery is greatly reduced, and the performance of the battery piece is improved.

The invention relates to a preparation method of a 3D printing high-density cell tubular vascular graft. The preparation method comprises the following steps: sucking a supernatant obtained by centrifuging a cell suspension away and only remaining bottom pure cells; the preparation method comprises the following steps: uniformly stirring and mixing OMA, pig gelatin and an LAP photoinitiator in a Hank's solution to form a hydrogel precursor solution; extruding the pure cells and the hydrogel precursor solution from a three-axis nozzle by using printing equipment, and immersing the pure cells and the hydrogel precursor solution into a calcium chloride solution; lyase is added to degrade the hydrogel precursor solution, the hydrogel precursor solution is completely degraded, and an independent single-layer pipeline structure with only a remaining pure cell layer is obtained. The invention provides a method for manufacturing high-density pure-cell lumen constructs to mimic native blood vessels by using a self-made microfluidic coaxial 3D bioprinting device. And after the support hydrogel on the inner layer and the outer layer is removed, a single-layer tubular structure of high-density MOVAs only limited to pure cells is obtained. The cells in the pure cell vascular constructs exhibit good cell viability and cell function.

The invention discloses a chemical labeling system (HIM-IP) for detecting interaction between cells. According to the system, tyrosinase and phenol are respectively modified on the surfaces of two to-be-detected cells to construct capturing cells and prey cells, and then biotin or single-stranded DNA with nucleophilic groups is used as a molecular tag for labeling. The system not only can perform detection through traditional flow cytometry, but also can be compatible with a commercialized single cell transcriptome sequencing platform to realize association of intercellular interaction information and transcriptome information on a single cell level. The system does not need to carry out complex gene engineering modification on cells, the whole modification and marking process is simple, convenient and efficient, the detection sensitivity of intercellular interaction is high, and the biological mechanism of intercellular interaction regulation and control can be deeply excavated by integrating the single cell sequencing technology.

A computing device includes at least one memory and at least one processor. The at least one processor is configured to detect a plurality of tumor cells included in one or more tumor areas (cancer areas) from a pathological slide image, determine a cell expression class of the plurality of tumor cells, based on a biomarker expression degree of the plurality of tumor cells, and generate a heatmap image for the pathological slide image, based on a result of the determining.

Provided is a method for screening myocardial therapeutic targets for ischemic heart failure by using single-cell sequencing, which method comprises the following steps: S1, sample preparation; S2, construction of a single-cell expression matrix; S3, cell quality control; S4, cell type annotation; S5, cell communication analysis; and S6, co-expression network analysis. The provided method for screening myocardial therapeutic targets for ischemic heart failure by using single-cell sequencing comprises performing single-cell sequencing on hearts of healthy mice and IHF mice, screening for cell types with significant differences in cardiac transcriptional profiles of the healthy mice and IHF mice, then exploring interaction characteristics of various types of cells in malignant fibrotic IHF hearts, revealing potential regulatory modules and pathways related to malignant myocardial fibrosis in single-cell expression data of IHF hearts, and performing screening to obtain Pdgfb and Tnfsf12 genes which can be used as therapeutic targets for treating myocardial fibrosis in ischemic heart failure.

The invention discloses a pichia pastoris high-secretion strain screening system and a use method thereof. According to the system, pichia pastoris is used as a host, and the core structure of the system is as follows: an AOX1 promoter, an alpha-factor signal peptide, a target protein-P2A peptide, an alpha-factor signal peptide, a Gamilus fluorescent protein-G4S connecting peptide-Gcw21 anchoring sequence-AOX1 terminator. The use method comprises the steps of vector construction, conversion of enzyme digestion recovery products, clone strain selection and activation, fermentation, inducible expression and screening, and product collection to finally obtain the strain with high yield of target protein. Through integration of bicistronic design, P2A peptide self-cleavage and cell surface display technologies, efficient screening of target protein high-secretion type strains is realized.

Provided is an anti-CLL1 antibody and an application thereof. The variable region of the anti-CLL1 antibody includes the CDRs of SEQ ID NO: 1 to 6, SEQ ID NO: 5, and SEQ ID NO: 7 to 11 or SEQ ID NO: 12 to 17. The anti-CLL1 antibody of the present application has significant binding ability to both free and cell surface CLL1. After humanization, the affinity of the antibody to CLL1 is further improved, and it has important application prospect in the clinical diagnosis and / or treatment of tumors.

The invention discloses an RNA (Ribonucleic Acid) binding protein enrichment analysis method based on single cell expression profile data, which comprises the following steps of: writing an interactive online Web application program by using Shiny, carrying a data visualization tool, and using an RBP (Reactive Binding Protein)-gene database as a database; the method is specially designed for single cell data, different single cell data can be integrated for enrichment analysis, and expression profiles and RBP dynamic changes in single cells can be effectively captured and analyzed; rBP enrichment analysis is customized for single cell data, a single group and multiple groups of single cell expression data are preprocessed, including normalization processing, and a statistical model is used to evaluate enrichment significance at each time point, so that the analysis accuracy and biological correlation are improved; a dynamic visualization tool is provided, the dynamic visualization tool comprises a customizable network diagram, an interactive bar diagram and detailed table display, and a user can adjust a plurality of parameters of a view according to needs and directly download images and data from an interface.

The invention discloses a recombinant strain for biosynthesis of gamma-aminobutyric acid as well as a construction method and application of the recombinant strain, and belongs to the technical field of gene engineering. In order to solve the technical problems that GAD (gamma-aminobutyric acid) is seriously limited to be synthesized by corynebacterium glutamicum by adopting a cell surface display technology because the growth of corynebacterium glutamicum and the production of GABA (gamma-aminobutyric acid) have difference on pH (potential of hydrogen) requirements and L-glutamic acid has a feedback inhibition effect on the synthesis process of the L-glutamic acid, the invention adopts a cell surface display technology to display GAD on the outer surface of a cell. Therefore, the GAD not only can exert higher activity in a more suitable meta-acidic environment, but also can directly convert a precursor L-glutamic acid continuously secreted to extracellular into extracellular GABA, and extracellular transport of the GABA does not need to be carried out any more. Furthermore, in order to more efficiently and simultaneously transform intracellular and extracellular L-glutamic acid, GAD is expressed in cells on the basis of displaying GAD on the surfaces of the cells, so that the L-glutamic acid is simultaneously transformed in the cells and extracellular, and synthesis of GABA (gamma-aminobutyric acid) is accelerated.

A photovoltaic module and a photovoltaic system. The photovoltaic module includes a panel, a back plate, a cell sheet layer, and a first encapsulation adhesive film layer, wherein the back plate and the panel are stacked, the cell sheet layer is located between the panel and the back plate, the first encapsulation adhesive film layer is located between the cell sheet layer and the panel, a color layer is provided between the panel and the first encapsulation adhesive film layer, the color layer is a light-transmitting layer, and a relief structure is provided on the side of the panel facing away from the first encapsulation adhesive film layer. The relief structure can reduce the reflection of sunlight on the panel, thereby increasing the incident amount of sunlight and increasing the power generation of solar energy.

The photovoltaic cell sheet includes: a cell sheet body having a first end and a second end opposite to each other in a width direction of the cell sheet body, wherein the first end of the cell sheet body is provided with a plurality of sawtooth spacing along a length direction of the cell sheet body; a first main gate arranged at one side of front and back sides of the plurality of sawtooth and serving as a first electrode; and a second main gate arranged at another side of the front and back sides of the cell sheet body and serving as a second electrode, wherein the second main gate is adjacent to the second end of the cell sheet body.

Disclosed are fusion proteins (and homodimers and heterodimers thereof). Fusion proteins can bind to a protein of interest and to an internalizing receptor (transferrin receptor) on a cell surface. Once bound, the protein of interest can be internalized and / or degraded inside a cell.

The invention relates to an IL5RA cell surface marker. A cell tag comprising an extracellular region, a transmembrane region, and optionally an intracellular region is provided. The extracellular region comprises an IL5 receptor alpha (IL5Ra) sequence linked to a transmembrane domain, and the recombinant polypeptide cannot function in signal transduction. The cell tag can be operably linked to a transgene. Expression of the cell tag allows for the identification, detection, selection, and ablation of cells expressing the transgene and the cell tag. In some embodiments, the genetically modified host cell comprises a transgene comprising a polynucleotide encoding a chimeric antigen receptor comprising a ligand binding domain and a polynucleotide encoding a cell tag. Pharmaceutical formulations produced by the methods and methods of using the pharmaceutical formulations are also described.

Methods for preparing a personalized cancer vaccine and a method to train a machine learning HLA-peptide presentation prediction model. Further wherein, a method of making a HLA class II tetramer or multimer comprising an epitope, the method comprising contacting a purified soluble HLA-DM loaded with a peptide epitope with a HLA class II tetramer or multimer, thereby forming a HLA class II tetramer or multimer loaded with the peptide epitope, is disclosed.

The invention discloses a method for preparing bovine MSTN gene editing cells by using CRISPR / Cas12i, and belongs to the technical field of biology. According to the invention, an sgRNA expression vector aiming at the bovine MSTN gene is constructed, and a CRISPR / Cas12i system is utilized to carry out precise gene editing on a specific target area of a first exon of the bovine primary fibroblast MSTN gene, so that mRNA of the MSTN gene cannot be normally transcribed, and MSTN gene deleted cells are obtained. Compared with other gene editing tools, the Cas12i system shows higher specificity and editing efficiency (improved by 3%-5%), and the off-target effect risk is effectively reduced. The prepared MSTN gene deleted bovine recombinant cell line is stable and efficient in editing efficiency, the cell expression level is not affected, the MSTN gene deleted bovine recombinant cell line can be widely applied to somatic cell cloning and microinjection preparation of double-muscle-hip beef cattle, and a new strategy is provided for beef cattle breeding.

A graft method according to the present invention comprises: a culture step in which a cell sheet is cultured on a culture surface of a culture carrier substrate; and a graft step in which the cell sheet formed on the culture surface of the culture carrier substrate is attached to a recipient site, after which the culture carrier substrate is detached from the cell sheet.

The invention belongs to the technical field of single cell data analysis, and particularly relates to a single cell pseudo-trajectory identification method based on a gene co-expression network. Comprising the following steps: S1, constructing a single cell transcription data matrix of behavior gene names and cell names; s2, preprocessing the single-cell transcription data matrix, constructing a cell expression matrix, and performing characteristic gene screening on the cell expression matrix to obtain a single-cell gene expression matrix; s3, constructing a gene co-expression network; s4, constructing a gene rank differential expression network in combination with the single-cell gene expression matrix and the gene co-expression network; and S5, carrying out integrated clustering on the gene rank differential expression network, fusing by using a similarity network to obtain a final clustering result, determining an initial cluster, distributing pseudo time for cells in each cluster, and deducing a cell pseudo trajectory. According to the method, the differentiation track of the cell can be identified, and compared with an original gene expression matrix, a more stable identification result can be obtained by utilizing a relative expression sequence.