998 results about "Bio molecules" patented technology

The present disclosure relates to a copolymer and a polymersome for targeted delivery of biomolecules to a living organism. The exemplary copolymer comprises an initiator block, a propagator block, and a linkage connecting the initiator block and the propagator block. The initiator block comprises a glycan head configured to provide a targeted delivery, and the propagator block comprises a functional moiety configured to provide desired properties for the polymersome.

The invention relates to an optical probe, in particular to a plasma optical sensing probe for online trace detection of biological threat factors. The sensing probe is composed of a microstructure optical fiber and a nano gold wire; the optical fiber is provided with two micro grooves which are symmetrically distributed, and the gold nanowires are located at the bottoms of the grooves; four inner-layer large air holes and two outer-layer small air holes are formed in the cladding of the optical fiber, and every two of the four inner-layer large air holes form a group and are symmetrically distributed relative to the groove; and the two outer-layer small air holes are symmetrically distributed relative to the micro groove. The radius of the optical fiber is 16 microns; the depth of the micro groove is 8 microns; the radius of the nano gold wire is 500 nm; the radius of each inner-layer large air hole is 4 microns, and among the four inner-layer large air holes, the horizontal circle center distance of the inner-layer large air holes is 13 microns, and the vertical circle center distance of the inner-layer large air holes is 11 microns; and the radius of the small air holes in the outer layer is 1.5 microns. According to the probe, efficient and accurate biomolecule detection is realized, and reliable technical support is provided for early diagnosis and timely treatment.

Provided herein are methods for minimizing Z-image acquisition comprising receiving a first set of three-dimensional (3D) positional information of a plurality of biological molecules within a sample, wherein the first set of 3D positional information is within a first imaging volume and based on a probing cycle of the sample in an imaging instrument, wherein the probing cycle comprises generating optical signals corresponding to at least some of the plurality of biological molecules; determining, based on the first set of 3D positional information, a second imaging volume that is less than the first imaging volume; and directing the imaging instrument to image the second imaging volume in at least one subsequent probing cycle of the sample.

The present disclosure relates to a copolymer and a polymersome for targeted delivery of biomolecules to a living organism. The exemplary copolymer comprises an initiator block, a propagator block, and a linkage connecting the initiator block and the propagator block. The initiator block comprises a glycan head configured to provide a targeted delivery, and the propagator block comprises a functional moiety configured to provide desired properties for the polymersome.

Systems and methods for generating a polymer sequence for a biological molecule having one or more target biological properties are provided. A plurality of target metric values for one or more target biological properties of a biological molecule and a seed for a nucleic acid or amino acid sequence for the biological molecule are inputted into a conditional generator model of a conditional generative adversarial network to obtain as output from the conditional generator model a nucleic acid or amino acid sequence for the biological molecule that is predicted by the conditional generator model to confer on the biological molecule the one or more target biological properties approximating the plurality of target metric values.

The invention discloses a method for constructing a hyperbolic discrete diffusion model with a three-dimensional RNA (Ribonucleic Acid) structure inversely folded, which comprises the following steps of: embedding rich geometrical characteristics and other topological properties of the RNA three-dimensional structure into a hyperbolic space; a forward diffusion process is utilized to carry out step-by-step noise addition on a real one-dimensional sequence of an RNA three-dimensional structure and carry out step-by-step noise removal on a hyperbolic isovariant graph neural network noise removal model, and on the basis of a limited training sample, nucleotide distribution of a target RNA three-dimensional structure, namely the one-dimensional sequence, is effectively recovered, so that the target RNA three-dimensional structure is obtained. According to the method, the hierarchical structure of RNA is represented through a hyperbolic isovariant graph neural network denoising model, and the structural similarity measurement of three-dimensional reverse folding is enhanced. The invention provides a powerful artificial intelligence method for the generative design of a functional RNA structure, and is beneficial to the design of RNA drugs and RNA biomolecules and the application of the RNA drugs and RNA biomolecules in biological medicines.

The invention discloses a complex sample detection method based on surface enhanced Raman spectroscopy and application, and relates to the field of biomolecular detection.The method comprises the following steps that silver nanoparticles are prepared and serve as a surface enhanced Raman substrate; preparing a concentrated silver particle solution by using the silver nanoparticles; detecting surface enhanced Raman spectrum signals of the complex sample under excitation of different wavelengths; removing a background signal and noise from the obtained surface enhanced Raman spectrum signal; and averaging the surface enhanced Raman spectra obtained by processing each wavelength, and carrying out maximum and minimum normalization processing on the obtained average spectrum intensity. According to the method, richer and more comprehensive chemical information and component information of the complex samples can be obtained, more accurate recognition and classification of the complex samples can be achieved, the recognition accuracy is greatly improved, the method is convenient and rapid to operate, low in cost and high in generalization ability and practicability, and large-scale commercial application can be achieved.

The invention belongs to the technical field of biomolecules, and particularly relates to an NIPBL gene for judging the sex of chickens and application of the NIPBL gene. The NIPBL gene has a segment with the difference of 606bp on chicken Z and W chromosomes, and is also a deletion segment on the W chromosome, the chicken sex can be quickly and effectively identified by detecting the sequence condition, the chicken with the deletion of the molecular marker sequence segment is a female individual, and the chicken without the deletion of the molecular marker sequence segment is a male individual; the invention further provides a specific primer, the chicken sex can be accurately judged through a PCR amplification technology and agarose gel electrophoresis, a strip is clear, the method is small in technical difficulty, easy and convenient to operate and high in accuracy, the sex of chicks or chickens difficult to distinguish in appearance can be effectively identified, the method has practical significance on identification of varieties small in male and female appearance difference, and the method is worthy of popularization and application. The method disclosed by the invention is strong in technical applicability and good in repeatability, can be used for accurately identifying the gender of the poultry, and has a wide application prospect.

The present invention relates to a lab-on-a-chip device with modular layout for determining a plurality of biological, molecular, chemical or physical parameters associated with soil, in particular soil health and quality. Also disclosed is method for using the device.

The invention belongs to the technical field of molecular identification of aquatic organisms, and particularly relates to a high-sensitivity large yellow croaker germplasm identification primer combination as well as application and an identification method thereof. The primer composition is a first pair of primers LC-1, and the sequences of the first pair of primers LC-1 are as follows: an upstream primer LC-1F: 5 '-AATTCCACACGACTAATC-3'; the downstream primer LC-1R is 5 '-CTCAGCCGCCGTGCAATC-3', and the downstream primer LC-1R is 5 '- The sequences of the second pair of primers LC-2 are as follows: an upstream primer LC-2F: 5 '-AGGCATTAGGCACAAGG-3', a downstream primer LC-2F: 5 '-AGGCATTAGGCACAAGG-3', a downstream And the downstream primer LC-2R is 5 '-AAACCCCTAGCCTCGAAG-3'. The invention also discloses a kit for detecting the disease resistance of the traditional Chinese medicine. The two large yellow croaker germplasm identification primers provided by the invention have relatively high sensitivity and specificity, can be stably amplified in a DNA sample with extremely low concentration, and overcome the limitation that accurate identification cannot be realized due to low DNA concentration in a traditional method.

The invention discloses a near-infrared fluorescent probe for detecting ONOO <-> as well as a preparation method and application of the near-infrared fluorescent probe, and belongs to the technical field of peroxynitroso ion detection. The near-infrared fluorescent probe for detecting ONOO <-> provided by the invention is high in detection speed, and the highest fluorescence intensity can be reached within 60 seconds; the reaction is obvious and easy to observe, when the compound reacts with ONOO <->, strong red fluorescence can be generated, and the change of the color of the solution can be observed by naked eyes; the selectivity is strong, the sensitivity is high, the interference of other biological molecules and active substances can be effectively avoided, the accuracy and the reliability of a detection result are ensured, and the lowest detection limit can reach 107nM; the hydrogel has good biocompatibility, is low in toxicity in a living body, and does not generate adverse effects on normal physiological functions of a biological sample; the provided preparation method is simple and easy to operate, the raw materials are low in cost and easy to obtain, the synthesis efficiency is high, and the preparation method can be used for large-scale industrial production and has wide application prospects.

The invention belongs to the technical field of biomolecules, and particularly relates to an SMAD7 gene for judging chicken sex and application. Through the molecular marker and the design of a new PCR primer, the migration distance difference of two bands amplified by a ZW chromosome is 299bp, and compared with the migration distance difference of 150bp of a traditional sex identification gene CHD1 amplification system, the migration distance difference of the bands of the two items is larger, and judgment is easier; the fragments amplified by the designed PCR primer are smaller and are only 517bp and 816p, the PCR amplification time is remarkably shortened, and the sex identification time is shortened; in addition, amplification and agarose gel electrophoresis can be directly carried out by extracting chicken tissues, the sex of the chicken can be rapidly judged according to an electrophoresis result, the DNA extraction time is shortened, the time length can be controlled within 1 hour, operation is easy, the identification result is accurate, basic-level application and popularization are facilitated, and the method has a wide market application prospect.

The invention provides a beta '-sulfo-alpha, beta-unsaturated gamma-lactam derivative as well as a preparation method and application thereof, and belongs to the technical field of organic chemical synthesis. The beta '-thio-alpha, beta-unsaturated gamma-lactam derivative has a structure as shown in a formula I. According to the method disclosed by the invention, cesium carbonate is taken as alkali, and a molecular skeleton of the beta'-thio-alpha, beta-unsaturated gamma-lactam derivative is constructed through free radical coupling and olefin isomerization processes under the condition of blue light irradiation. Bioactivity evaluation shows that the beta '-sulfo-alpha, beta-unsaturated gamma-lactam derivative prepared by the method disclosed by the invention has an inhibition effect on MDA-MB-231 human breast cancer cells.

The invention belongs to the field of synthesis of nano materials, and particularly relates to a core-shell structure gold nano star and a preparation method and application thereof, and the core-shell structure gold nano star comprises a first gold nano star containing a metal sulfide shell layer and a second gold nano star containing a metal oxide shell layer. The preparation method comprises the following steps: firstly preparing a gold nano star core solution, and then respectively adding different precursors to prepare two core-shell structure gold nano stars. The method has the advantages of simple synthesis conditions and non-toxic reagents. The core-shell structure gold nanostars are used for preparing a detection solution based on a buffer solution, a color developing agent and an oxidizing agent, the detection solution reacts with antioxidants, a data matrix is constructed by measuring absorbance changes and is analyzed, single, binary and ternary combinations of various antioxidants such as ascorbic acid and glutathione can be accurately distinguished, and the detection accuracy is improved. The application prospect in the fields of biomolecules and food detection is good.

The invention provides a perovskite solar cell, a preparation method thereof and a photovoltaic module. The perovskite solar cell comprises a first electrode, a hole transport layer, a perovskite layer, an electron transport layer and a second electrode which are stacked in sequence. The material of the hole transport layer comprises a biomolecular material which is fixed on a self-assembly monomolecular layer material through an intermolecular force, and the biomolecular material comprises one or more of guanine, adenine, thymine, uracil, cytosine, hydroxymethylcytosine, xanthine, hypoxanthine, 6-mercaptopurine and corrin. According to the perovskite cell provided by the invention, the biomolecular material with a plurality of nitrogen heterocyclic structures and amino groups is added to generate multipoint hydrogen-bond interaction and pi-pi accumulation with the tail end of the SAM, so that the orderliness of molecular arrangement is improved, and the dipole direction and strength of the whole interface are regulated and controlled; and a coordination bond or hydrogen bond network is formed by the coordination compound and uncoordinated cations or anions in perovskite, so that interface defects are effectively passivated, and the thermal stability and the humid and hot life of a device are enhanced.

Methods, systems, and devices for enumerating the number of cells present in each event for cell-secretion based sorting applications are described herein. The cell secretion applications use carrier to encapsulate cells and collect the biomolecules they secrete. The carriers are then sorted using flow based particle sorting. The total number of cells present in a carrier is very important for several applications. A convolutional neural network is used to count the number of cells present from a brightfield image, and outputs the information to be used as part of the sort logic.

The present invention provides methods and compositions for targeted delivery of chemical or biomolecular cargo and / or nanoparticles to plant phloem or fungi.

An evaporation device includes a sample fluid channel extending from a sample fluid inlet to a sample fluid outlet. The evaporation device is configured to evaporate a solvent of a sample fluid containing a target analyte to increase the concentration of the target analyte. The device includes a housing enclosing and defining a testing environment within which the evaporation device is disposed. The housing includes a heater, a temperature sensor, and a humidity sensor. A pump coupled to a gas inlet tube is configured to cause a gas flow within the testing environment adjacent to the evaporation device. A controller in communication with each of the heater, the temperature sensor, and the humidity sensor, is configured to activate the heater in response to at least one of a temperature value or a humidity value of the testing environment.

A computer-implemented method can include: receiving input of a biological target; receiving a generative model (e.g., tensorial reinforcement learning (GENTRL) model or other model) trained with reference compounds, wherein the reference compounds include: general compounds, compounds that modulate the biological target, and compounds that modulate biomolecules other than the biological target; generating structures of generated compounds with the generative model; prioritizing structures of generated compounds based on at least one criteria; processing prioritized chemical structures of the generated compounds through a Sammon mapping protocol to obtain hit structures; and providing chemical structures of the hit structures. One or more non-transitory computer readable media are provided that store instructions that in response to being executed by one or more processors, cause a computer system to perform operations, the operations comprising performing the computer methods described herein for providing chemical structure of hit structures generated by the generative model.

The invention provides a photoelectrochemical biosensor based on a porous membrane as well as a preparation method and application of the photoelectrochemical biosensor, and belongs to the field of photoelectrochemical biosensors. The photoelectrochemical biosensor comprises an electrode, the electrode comprises ITO conductive glass and a composite film coated on the ITO conductive glass, the composite film is obtained by modifying a photosensitive material in a nano-pore channel of a porous film and then connecting a capture probe in the nano-pore channel, and the photoelectrochemical biosensor based on the porous film is obtained. The sensor has the following advantages: 1, other biomolecules can be detected by changing the type of the capture probe, and the sensor has excellent practical application value; 2, the ITO conductive glass is loaded with the porous membrane to replace a traditional metal electrode, so that the detection is accurate, the detection time is short, the cost is low, and the reproducibility and the stability are good;

The present invention discloses a flow type fluorescence detection system, which comprises: a sample flow channel, which is used for conveying a target object specifically bound with a nano-diamond mark, the nano-diamond comprises an NV color center, and the sample flow channel is provided with a detection area; the exciting light module is used for exciting an NV color center of a detection area of the sample flow channel, so that electrons of the NV color center are transited to generate fluorescence; the microwave radiation module is used for providing a microwave field for a detection area of the sample runner; the optical detection module is used for collecting a target material which is specifically bound with the nano-diamond mark at a preset collection frequency, and when the target material flows through the detection area of the sample flow channel, the NV color center in the nano-diamond emits a fluorescence signal of which the intensity is periodically changed by controlling at least one of the intensity, the frequency and the on-off of a microwave field; the fluorescence signal is associated with whether the target contains to-be-detected biomolecules or not. According to the technical scheme provided by the embodiment of the invention, the detection sensitivity of the fluorescence detection system is improved.

The invention discloses a preparation method and application of a sensor array based on luminous carbon dots, and relates to the technical field of sensors, the preparation method comprises the following steps: preparing a carbon source which comprises a precursor containing a targeting functional group or a biomolecule derived carbon source, preparing the luminous carbon dots through a chemical synthesis or physical method, modifying the surfaces of the carbon dots, and preparing the sensor array based on the luminous carbon dots. A core-shell structure is added on the surface of a carbon dot by using an in-situ growth method or a layer-by-layer self-assembly method, the carbon dot with the added core-shell structure is fixed on a substrate of a sensor array, and a performance test is carried out. By synthesizing the carbon dots with a targeted recognition function, the selectivity and the sensitivity of the sensor to a target substance can be remarkably improved, and in the synthesis process, the carbon dots can generate specific interaction with the target substance by adjusting factors such as the structure, the shape, the size and the surface chemical property of the carbon dots; therefore, accurate identification of the target substance is realized, and detection of the marker is realized.

The disclosure relates to the field of specialty chemicals and methods for their synthesis. In embodiments, the disclosure provides viable bacterial cells which comprise heterologous dual 3-hydroxy-acyl-ACP dehydratase / isomerases, etc. The disclosure further provides monounsaturated fatty acid derivative molecules produced by the viable bacterial cells which are non-native to the bacterial cells. The disclosure further provides methods for the preparation and production of non-native monounsaturated fatty acid derivative molecules such as e.g., an ω3-monounsaturated fatty acid derivative, an ω5-monounsaturated fatty acid derivative, an ω9-monounsaturated fatty acid derivative, an ω11-monounsaturated fatty acid fatty acid derivative, etc.

The invention discloses a surface plasmon resonance imaging system for expanding a dynamic range through a multi-mode optical path, and relates to the technical field of microscopic imaging, a laser outputs a monochromatic and linearly polarized continuous wave beam, a first dynamic beam deflection device performs dynamic angle deflection adjustment on the continuous wave beam, a prism is used for providing a total reflection interface, and a second dynamic beam deflection device is used for performing dynamic angle deflection adjustment on the continuous wave beam. Exciting an evanescent wave; a surface plasma resonance effect is generated through interaction of the SPR detection chip and evanescent waves, and sample characteristics are detected; the flow cell provides a sample flow channel, so that a sample is in full contact with the surface of the SPR detection chip; the second dynamic light beam deflection device cooperatively adjusts the propagation direction of reflected light on the surface of the SPR detection chip, and the imaging detection device captures a high-spatial-resolution SPR image formed by the reflected light and transmits the image to the upper computer in real time for processing and analysis. According to the invention, the change of the resonance angle can be tracked in real time, and high-sensitivity and high-resolution monitoring of the dynamic process of biomolecule interaction is realized.

The invention discloses a near-infrared fluorescent probe DCM-MBS for detecting ONOO <-> as well as a preparation method and application of the near-infrared fluorescent probe DCM-MBS, and belongs to the technical field of peroxynitroso ion detection. The probe DCM-MBS is a water-soluble probe, the detection method is simple, ONOO <-> response is rapid, selectivity is good, specificity is high, detection sensitivity is high, interference of other biological molecules, active substances and the background of the probe can be effectively avoided, repeatability, accuracy and reliability of a detection result are ensured, and the detection method is suitable for large-scale popularization and application. Further, the requirement of real-time monitoring of the rapidly-changed ONOO <-> level in a living body is met; the color change is obvious and easy to observe, and qualitative in-situ real-time detection of ONOO <-> can be realized by naked eyes; the fluorescent probe has very excellent biocompatibility, can realize excellent fluorescence imaging in various cells and living mouse bodies, and does not have obvious toxic or side effects; the synthesis method is simple and efficient, raw materials are low in cost and easy to obtain, large-scale industrial production is facilitated, and wide market application prospects are achieved.

The invention provides a sampler and a sampling method for collecting biological molecules on the surface of tissue, the sampler is provided with a sampling direction, the sampler comprises a main body, the main body is provided with an arc-shaped track, the center of the arc-shaped track corresponds to the center of the main body, and the sampling direction of the main body is equal to that of the arc-shaped track in one length direction of the track. And the radius of the arc-shaped track is gradually increased or decreased. According to the sampler for collecting the biological molecules on the surface of the tissue, a plurality of sampling needles are arranged, a sampling liquid drop corresponding to a sampling center can be formed and maintained among the output ends of the plurality of sampling needles, and a non-embedded sampling mode is realized through contact between the liquid drop and the surface of the biological tissue; the damage to the integrity of key biomolecules possibly caused by a traditional method is avoided, the acquisition of high-fidelity biomolecules is facilitated, and the sampler can reduce physical disturbance and mechanical damage to fragile tissues to the maximum extent.

A filter assembly includes a housing having an inlet port, an outlet port, and an interior compartment, the inlet port and the outlet port fluidically coupled to form a flow path in the interior compartment, a filter membrane in the flow path, the filter membrane having a porosity capable of capturing biological molecules as liquid flows along the flow path, a fluid screen in the flow path on an opposite side of the filter membrane from the inlet port, and a desiccating element in the flow path comprised of one of a hygroscopic or hydrophilic material selected to absorb moisture from the filter membrane at ambient temperature.

The present invention provides a heparin affinity chromatography medium and a preparation method thereof. The heparin affinity chromatography medium comprises a porous polymer microsphere matrix modified with a polyamide-amine dendrimer to obtain a modified matrix; heparin is coupled to the modified matrix to obtain the heparin affinity chromatography medium. The heparin affinity chromatography medium of the present invention has a high adsorption capacity for target biomolecules, significantly improving separation efficiency; and also exhibits high mechanical strength.

The present disclosure provides engineered bacterial cells having one or more genetic modifications that result in increased production of expression products, such as increased production of plasmids with reduced occurrence of plasmid concatemers or multimers. Also disclosed herein are expression systems for product manufacturing in media having reduced antibiotic concentration, as well as methods of making cells for use in the expression systems. The cells and expression systems increase the growth rate and production yields of biomolecules produced within the host bacterial host cells without the use of antibiotics, and can produce the product with little to no endotoxins present.

The present disclosure provides engineered bacterial cells having one or more genetic modifications that result in increased production of expression products, such as increased production of plasmids with reduced occurrence of plasmid concatemers or multimers. Also disclosed herein are expression systems for product manufacturing in media having reduced antibiotic concentration, as well as methods of making cells for use in the expression systems. The cells and expression systems increase the growth rate and production yields of biomolecules produced within the host bacterial host cells without the use of antibiotics, and can produce the product with little to no endotoxins present.