683 results about "Bio molecules" patented technology

The invention relates to an optical probe, in particular to a plasma optical sensing probe for online trace detection of biological threat factors. The sensing probe is composed of a microstructure optical fiber and a nano gold wire; the optical fiber is provided with two micro grooves which are symmetrically distributed, and the gold nanowires are located at the bottoms of the grooves; four inner-layer large air holes and two outer-layer small air holes are formed in the cladding of the optical fiber, and every two of the four inner-layer large air holes form a group and are symmetrically distributed relative to the groove; and the two outer-layer small air holes are symmetrically distributed relative to the micro groove. The radius of the optical fiber is 16 microns; the depth of the micro groove is 8 microns; the radius of the nano gold wire is 500 nm; the radius of each inner-layer large air hole is 4 microns, and among the four inner-layer large air holes, the horizontal circle center distance of the inner-layer large air holes is 13 microns, and the vertical circle center distance of the inner-layer large air holes is 11 microns; and the radius of the small air holes in the outer layer is 1.5 microns. According to the probe, efficient and accurate biomolecule detection is realized, and reliable technical support is provided for early diagnosis and timely treatment.

The present disclosure relates to a copolymer and a polymersome for targeted delivery of biomolecules to a living organism. The exemplary copolymer comprises an initiator block, a propagator block, and a linkage connecting the initiator block and the propagator block. The initiator block comprises a glycan head configured to provide a targeted delivery, and the propagator block comprises a functional moiety configured to provide desired properties for the polymersome.

The invention discloses a near-infrared fluorescent probe for detecting ONOO <-> as well as a preparation method and application of the near-infrared fluorescent probe, and belongs to the technical field of peroxynitroso ion detection. The near-infrared fluorescent probe for detecting ONOO <-> provided by the invention is high in detection speed, and the highest fluorescence intensity can be reached within 60 seconds; the reaction is obvious and easy to observe, when the compound reacts with ONOO <->, strong red fluorescence can be generated, and the change of the color of the solution can be observed by naked eyes; the selectivity is strong, the sensitivity is high, the interference of other biological molecules and active substances can be effectively avoided, the accuracy and the reliability of a detection result are ensured, and the lowest detection limit can reach 107nM; the hydrogel has good biocompatibility, is low in toxicity in a living body, and does not generate adverse effects on normal physiological functions of a biological sample; the provided preparation method is simple and easy to operate, the raw materials are low in cost and easy to obtain, the synthesis efficiency is high, and the preparation method can be used for large-scale industrial production and has wide application prospects.

The invention provides a beta '-sulfo-alpha, beta-unsaturated gamma-lactam derivative as well as a preparation method and application thereof, and belongs to the technical field of organic chemical synthesis. The beta '-thio-alpha, beta-unsaturated gamma-lactam derivative has a structure as shown in a formula I. According to the method disclosed by the invention, cesium carbonate is taken as alkali, and a molecular skeleton of the beta'-thio-alpha, beta-unsaturated gamma-lactam derivative is constructed through free radical coupling and olefin isomerization processes under the condition of blue light irradiation. Bioactivity evaluation shows that the beta '-sulfo-alpha, beta-unsaturated gamma-lactam derivative prepared by the method disclosed by the invention has an inhibition effect on MDA-MB-231 human breast cancer cells.

The invention belongs to the field of synthesis of nano materials, and particularly relates to a core-shell structure gold nano star and a preparation method and application thereof, and the core-shell structure gold nano star comprises a first gold nano star containing a metal sulfide shell layer and a second gold nano star containing a metal oxide shell layer. The preparation method comprises the following steps: firstly preparing a gold nano star core solution, and then respectively adding different precursors to prepare two core-shell structure gold nano stars. The method has the advantages of simple synthesis conditions and non-toxic reagents. The core-shell structure gold nanostars are used for preparing a detection solution based on a buffer solution, a color developing agent and an oxidizing agent, the detection solution reacts with antioxidants, a data matrix is constructed by measuring absorbance changes and is analyzed, single, binary and ternary combinations of various antioxidants such as ascorbic acid and glutathione can be accurately distinguished, and the detection accuracy is improved. The application prospect in the fields of biomolecules and food detection is good.

The invention provides a perovskite solar cell, a preparation method thereof and a photovoltaic module. The perovskite solar cell comprises a first electrode, a hole transport layer, a perovskite layer, an electron transport layer and a second electrode which are stacked in sequence. The material of the hole transport layer comprises a biomolecular material which is fixed on a self-assembly monomolecular layer material through an intermolecular force, and the biomolecular material comprises one or more of guanine, adenine, thymine, uracil, cytosine, hydroxymethylcytosine, xanthine, hypoxanthine, 6-mercaptopurine and corrin. According to the perovskite cell provided by the invention, the biomolecular material with a plurality of nitrogen heterocyclic structures and amino groups is added to generate multipoint hydrogen-bond interaction and pi-pi accumulation with the tail end of the SAM, so that the orderliness of molecular arrangement is improved, and the dipole direction and strength of the whole interface are regulated and controlled; and a coordination bond or hydrogen bond network is formed by the coordination compound and uncoordinated cations or anions in perovskite, so that interface defects are effectively passivated, and the thermal stability and the humid and hot life of a device are enhanced.

Methods, systems, and devices for enumerating the number of cells present in each event for cell-secretion based sorting applications are described herein. The cell secretion applications use carrier to encapsulate cells and collect the biomolecules they secrete. The carriers are then sorted using flow based particle sorting. The total number of cells present in a carrier is very important for several applications. A convolutional neural network is used to count the number of cells present from a brightfield image, and outputs the information to be used as part of the sort logic.

The present invention provides methods and compositions for targeted delivery of chemical or biomolecular cargo and / or nanoparticles to plant phloem or fungi.

An evaporation device includes a sample fluid channel extending from a sample fluid inlet to a sample fluid outlet. The evaporation device is configured to evaporate a solvent of a sample fluid containing a target analyte to increase the concentration of the target analyte. The device includes a housing enclosing and defining a testing environment within which the evaporation device is disposed. The housing includes a heater, a temperature sensor, and a humidity sensor. A pump coupled to a gas inlet tube is configured to cause a gas flow within the testing environment adjacent to the evaporation device. A controller in communication with each of the heater, the temperature sensor, and the humidity sensor, is configured to activate the heater in response to at least one of a temperature value or a humidity value of the testing environment.

A computer-implemented method can include: receiving input of a biological target; receiving a generative model (e.g., tensorial reinforcement learning (GENTRL) model or other model) trained with reference compounds, wherein the reference compounds include: general compounds, compounds that modulate the biological target, and compounds that modulate biomolecules other than the biological target; generating structures of generated compounds with the generative model; prioritizing structures of generated compounds based on at least one criteria; processing prioritized chemical structures of the generated compounds through a Sammon mapping protocol to obtain hit structures; and providing chemical structures of the hit structures. One or more non-transitory computer readable media are provided that store instructions that in response to being executed by one or more processors, cause a computer system to perform operations, the operations comprising performing the computer methods described herein for providing chemical structure of hit structures generated by the generative model.

The present invention discloses a flow type fluorescence detection system, which comprises: a sample flow channel, which is used for conveying a target object specifically bound with a nano-diamond mark, the nano-diamond comprises an NV color center, and the sample flow channel is provided with a detection area; the exciting light module is used for exciting an NV color center of a detection area of the sample flow channel, so that electrons of the NV color center are transited to generate fluorescence; the microwave radiation module is used for providing a microwave field for a detection area of the sample runner; the optical detection module is used for collecting a target material which is specifically bound with the nano-diamond mark at a preset collection frequency, and when the target material flows through the detection area of the sample flow channel, the NV color center in the nano-diamond emits a fluorescence signal of which the intensity is periodically changed by controlling at least one of the intensity, the frequency and the on-off of a microwave field; the fluorescence signal is associated with whether the target contains to-be-detected biomolecules or not. According to the technical scheme provided by the embodiment of the invention, the detection sensitivity of the fluorescence detection system is improved.

The invention discloses a preparation method and application of a sensor array based on luminous carbon dots, and relates to the technical field of sensors, the preparation method comprises the following steps: preparing a carbon source which comprises a precursor containing a targeting functional group or a biomolecule derived carbon source, preparing the luminous carbon dots through a chemical synthesis or physical method, modifying the surfaces of the carbon dots, and preparing the sensor array based on the luminous carbon dots. A core-shell structure is added on the surface of a carbon dot by using an in-situ growth method or a layer-by-layer self-assembly method, the carbon dot with the added core-shell structure is fixed on a substrate of a sensor array, and a performance test is carried out. By synthesizing the carbon dots with a targeted recognition function, the selectivity and the sensitivity of the sensor to a target substance can be remarkably improved, and in the synthesis process, the carbon dots can generate specific interaction with the target substance by adjusting factors such as the structure, the shape, the size and the surface chemical property of the carbon dots; therefore, accurate identification of the target substance is realized, and detection of the marker is realized.

The invention discloses a surface plasmon resonance imaging system for expanding a dynamic range through a multi-mode optical path, and relates to the technical field of microscopic imaging, a laser outputs a monochromatic and linearly polarized continuous wave beam, a first dynamic beam deflection device performs dynamic angle deflection adjustment on the continuous wave beam, a prism is used for providing a total reflection interface, and a second dynamic beam deflection device is used for performing dynamic angle deflection adjustment on the continuous wave beam. Exciting an evanescent wave; a surface plasma resonance effect is generated through interaction of the SPR detection chip and evanescent waves, and sample characteristics are detected; the flow cell provides a sample flow channel, so that a sample is in full contact with the surface of the SPR detection chip; the second dynamic light beam deflection device cooperatively adjusts the propagation direction of reflected light on the surface of the SPR detection chip, and the imaging detection device captures a high-spatial-resolution SPR image formed by the reflected light and transmits the image to the upper computer in real time for processing and analysis. According to the invention, the change of the resonance angle can be tracked in real time, and high-sensitivity and high-resolution monitoring of the dynamic process of biomolecule interaction is realized.

The invention discloses a near-infrared fluorescent probe DCM-MBS for detecting ONOO <-> as well as a preparation method and application of the near-infrared fluorescent probe DCM-MBS, and belongs to the technical field of peroxynitroso ion detection. The probe DCM-MBS is a water-soluble probe, the detection method is simple, ONOO <-> response is rapid, selectivity is good, specificity is high, detection sensitivity is high, interference of other biological molecules, active substances and the background of the probe can be effectively avoided, repeatability, accuracy and reliability of a detection result are ensured, and the detection method is suitable for large-scale popularization and application. Further, the requirement of real-time monitoring of the rapidly-changed ONOO <-> level in a living body is met; the color change is obvious and easy to observe, and qualitative in-situ real-time detection of ONOO <-> can be realized by naked eyes; the fluorescent probe has very excellent biocompatibility, can realize excellent fluorescence imaging in various cells and living mouse bodies, and does not have obvious toxic or side effects; the synthesis method is simple and efficient, raw materials are low in cost and easy to obtain, large-scale industrial production is facilitated, and wide market application prospects are achieved.

The invention provides a sampler and a sampling method for collecting biological molecules on the surface of tissue, the sampler is provided with a sampling direction, the sampler comprises a main body, the main body is provided with an arc-shaped track, the center of the arc-shaped track corresponds to the center of the main body, and the sampling direction of the main body is equal to that of the arc-shaped track in one length direction of the track. And the radius of the arc-shaped track is gradually increased or decreased. According to the sampler for collecting the biological molecules on the surface of the tissue, a plurality of sampling needles are arranged, a sampling liquid drop corresponding to a sampling center can be formed and maintained among the output ends of the plurality of sampling needles, and a non-embedded sampling mode is realized through contact between the liquid drop and the surface of the biological tissue; the damage to the integrity of key biomolecules possibly caused by a traditional method is avoided, the acquisition of high-fidelity biomolecules is facilitated, and the sampler can reduce physical disturbance and mechanical damage to fragile tissues to the maximum extent.

The present invention provides a heparin affinity chromatography medium and a preparation method thereof. The heparin affinity chromatography medium comprises a porous polymer microsphere matrix modified with a polyamide-amine dendrimer to obtain a modified matrix; heparin is coupled to the modified matrix to obtain the heparin affinity chromatography medium. The heparin affinity chromatography medium of the present invention has a high adsorption capacity for target biomolecules, significantly improving separation efficiency; and also exhibits high mechanical strength.

The present disclosure provides engineered bacterial cells having one or more genetic modifications that result in increased production of expression products, such as increased production of plasmids with reduced occurrence of plasmid concatemers or multimers. Also disclosed herein are expression systems for product manufacturing in media having reduced antibiotic concentration, as well as methods of making cells for use in the expression systems. The cells and expression systems increase the growth rate and production yields of biomolecules produced within the host bacterial host cells without the use of antibiotics, and can produce the product with little to no endotoxins present.

The present disclosure provides engineered bacterial cells having one or more genetic modifications that result in increased production of expression products, such as increased production of plasmids with reduced occurrence of plasmid concatemers or multimers. Also disclosed herein are expression systems for product manufacturing in media having reduced antibiotic concentration, as well as methods of making cells for use in the expression systems. The cells and expression systems increase the growth rate and production yields of biomolecules produced within the host bacterial host cells without the use of antibiotics, and can produce the product with little to no endotoxins present.

The invention provides an immobilized carrier based on bovine serum albumin-biotin as well as a preparation method and application of the immobilized carrier. The biological sample fixing carrier comprises a substrate and a composite layer of bovine serum albumin coupled biotin and neutral avidin, wherein the composite layer is fixed on the surface of the substrate; the bovine serum albumin coupled biotin and neutral avidin composite layer is connected to the surface of the substrate after being co-incubated with the substrate through bovine serum albumin coupled biotin, and then the neutral avidin is combined with biotin in the bovine serum albumin coupled biotin through co-incubation with the neutral avidin; the bovine serum albumin coupled biotin and neutral avidin composite layer is fixed on the surface of the substrate. The biomolecules on the surface of the carrier are high in fixing efficiency, good in uniformity, high in adaptability, high in signal-to-noise ratio and easy to operate.

The invention relates to an anthrax virus protein nanopore buffer solution system and biomolecule detection application, and belongs to the field of nanopore electrochemistry. The invention aims to solve the problem that an anthrax virus protein nanopore is unstable in electrochemical detection, and provides an anthrax virus protein nanopore buffer solution system and application thereof. The buffer solution is composed of trihydroxymethyl aminomethane and a compound I composed of specific cations and anions, the compound I comprises the cations such as NH4 < + >, K < + >, Li < + > and Mg < 2 + > and the anions such as Cl <->, HCOO <-> and SO4 < 2->, and the stability of nanopores is achieved by optimizing the concentration and the pH value. The method is applied to high-sensitivity and high-selectivity detection of different charged small molecules. According to the technical scheme, the stability of the anthrax virus protein nanopore is remarkably improved, the application prospect of the anthrax virus protein nanopore in the field of single molecule detection is widened, and a new thought and method are provided for development of a nanopore sensing technology.

Disclosed herein, inter alia, are methods and systems of image analysis useful for identifying and / or quantifying features associated with biomolecules.

The invention discloses a rapid detection method for traumatic brain injury by fusing genetic engineering and quantum dots, and relates to the technical field of traumatic brain injury detection. The method comprises the following steps: acquiring a suspected patient biological sample, extracting biomolecule components, and comparing the biomolecule components with a healthy population biomolecule database to obtain related differential expression molecules. A specifically recognizable recombinant antibody is constructed by genetic engineering, and is connected with a quantum dot through a coupling reaction to prepare a detection probe. The probe and a biological sample are mixed and reacted, and the intensity of a fluorescence signal generated by combination is collected by a fluorescence detection device. A standardized signal value is obtained through signal processing algorithm noise reduction and feature extraction, and a preliminary detection result is determined by combining a bioinformatics database evaluation sample. And finally, according to the differential expression molecule, the standardized signal value and the preliminary result, analyzing a data change trend in a preset time window by using a multivariable statistical model, and generating a diagnosis report. According to the method, various technologies are fused, and a new path is provided for traumatic brain injury detection.

A magnetic tunneling junction (MTJ) device structure and the method of constructing such device are disclosed. Also disclosed are methods of using the device for spin-dependent transport characterization through biomolecules for structure and dynamic function analysis in physiological environments.

The invention discloses a pathogenic microorganism detection system based on nanopore sequencing and intelligent interpretation. The system comprises six modules including a biomolecule electric signal acquisition module and a pathogen characteristic sequence extraction module. Ion current change signals are collected through a nanopore chip array, a pathogen characteristic sequence is extracted through differential coding, a hypergraph topological structure self-adaption building module is used for building a pathogen relation model, a multi-dimensional heterogeneous pathogen parameter fusion analysis module integrates morphological parameters, metabonomics parameters and other multi-dimensional parameters, and the multi-dimensional heterogeneous pathogen parameter fusion analysis module is used for analyzing the multi-dimensional parameters. The optimized hypergraph neural network reasoning module realizes pathogen category prediction, and finally, the nanopore sequencing result intelligent interpretation output module generates a detection report containing pathogen categories, subtypes and risk levels. The system is combined with nanopore sequencing and an intelligent algorithm, pathogenic microorganism detection can be efficiently and accurately completed, the accuracy, reliability and efficiency of detection are greatly improved, and the requirement for rapid and accurate detection of pathogens in practical application is met.

The invention discloses a preparation and purification method and application of a quinoline derivative with biological activity, and belongs to the technical field of medicine synthesis. According to the key points of the technical scheme, the quinoline derivative molecule has a structure # imgabs0 #, wherein X is NH or empty (benzene rings are directly connected); r is an aromatic ring derivative or alkyl or other groups. The invention designs and synthesizes a quinoline derivative with a novel structure, and the compound has a relieving effect on LPS-induced microglial cell inflammation and has a certain inhibition effect on cervical cancer cells.

The invention discloses a nanoparticle scattered light confocal imaging device and method. The device comprises a laser unit, a first pinhole, a beam splitter turntable, a scanning focusing unit, an electric objective table, a first detection imaging unit, a second pinhole, a light splitting unit and a second detection imaging unit. The method does not need to mark nano particles, and can synchronously collect unmarked nano particles and fluorescence-labeled biomolecules; in-situ imaging can be realized, and the distribution position and content of nano-particles in living cells can be directly observed; the imaging resolution ratio is high, single nanoparticle imaging can be carried out, and fluorescence imaging can also be carried out at the biomolecule level.

The invention belongs to the technical field of biomolecular markers, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker related to the crown tooth number character of broiler chicken as well as a primer and application of the SNP molecular marker. Through phenotypic determination and whole genome re-sequencing related to crown tooth number characters of broiler individuals and whole genome association analysis, the excavated Chr7: 391959 locus and the crown tooth number have extremely high genetic correlation, the significance reaches 2.90 * 10 <-7 >, phenotypic variation variances capable of being explained reach 1.75%, the genetic effect is very remarkable, and the method has the advantages that the method is simple and convenient to operate, and the method is suitable for popularization and application. Therefore, the molecular marker can be effectively used for molecular marker-assisted selective breeding of the crown tooth number character of the broiler chicken, individuals with the molecular marker Chr7391959 site as the TT genotype are selected as breeding hens, the genetic selection process of the crown tooth number character of the yellow feather broiler chicken can be accelerated, the individuals are directly bred on the genome level in the early stage, the breeding efficiency is improved, and the breeding cost is reduced. The industrial competitiveness of the yellow feather broilers is effectively improved.

The invention provides a sample application method and a sample application device for a biological chip. The sample application method for the biological chip comprises the following steps: placing a biological molecule solution with a preset volume at a preset position on a solid-phase support in a contact or non-contact manner to form a sample application point array; wherein the sample application point array comprises a plurality of sample application points which are uniformly or basically uniformly distributed, each sample application point comprises a tiny sample application point group, and each tiny sample application point group comprises a plurality of tiny sample application points which are uniformly or basically uniformly distributed so as to inhibit the coffee ring effect. According to the biological chip spotting method provided by the invention, a single spotting point is decomposed into a plurality of tiny spotting points, so that the problem that the signal uniformity of a detection site is influenced by a coffee ring effect is solved, the detection signal is more uniform, and in addition, the detection point signal can be accurately determined after the coffee ring effect is overcome, so that quantitative analysis can be realized; and the application scene of the biochip is expanded.

The invention discloses application of a polymer coupled with a flavin group to catalysis of conversion of NADH (nicotinamide adenine dinucleotide) in a reduction state into NAD + (nicotinamide adenine dinucleotide) in an oxidation state, which is characterized in that in a solution containing various components, the polymer coupled with the flavin group catalyzes the NADH to convert the NAD +, the conversion rate is 0.007-0.85 mu M / s, and the conversion efficiency is 20-100%; the polymer coupled with the flavin group is a product obtained by coupling flavin with a polymer containing a cationic group side group through a covalent bond, the molar ratio of the flavin group in a product molecule to the cationic group in the polymer is 1: 1-1: 100, and the polymer is composed of a repetitive unit containing the cationic group and other repetitive units. Wherein the molar fraction of the amino-containing repeating unit is 5%-100%. Compared with riboflavin, flavin mononucleotide and other biomolecules containing flavin groups, the activity of catalyzing NADH to be oxidized into NAD + can be improved by at least 50 times or above.

The invention discloses a flow-type fluorescence detection method and system based on a zero field. The method comprises the following steps: when a target material specifically bound with a nano-diamond mark flows through a detection area of a sample flow channel, detecting the target material; by controlling at least one of the intensity, the frequency and the on-off of the microwave field, the NV color center in the nano-diamond emits a fluorescence signal with the intensity changing periodically; the magnetic field intensity of the detection area meets the zero field condition, the excitation light module is used for exciting the NV color center of the detection area of the sample flow channel, the microwave radiation module is used for providing a microwave field for the detection area of the sample flow channel, and a microwave radiation signal provided by the microwave field has a fixed frequency; the light detection module collects a fluorescence signal at a preset collection frequency; and the data processing module judges whether the target object contains the to-be-detected biomolecules according to the fluorescence signal. According to the technical scheme provided by the embodiment of the invention, the detection sensitivity of the fluorescence detection method is improved.