79 results about "Bio molecules" patented technology

The present application relates to a controllable preparation method of a colloidal crystal capillary column and its application in biomolecule separation, and specifically comprises the following steps: a plunger and an electroosmotic pump are prepared in situ at one end of a capillary; a buffer solution containing monodisperse colloidal particles is filled in an upper reservoir of an electric field assembly device, and a buffer solution is filled in a lower reservoir; the capillary is filled with the buffer solution, and the end of the capillary containing the electroosmotic pump is inserted into the lower reservoir; the dispersion of the colloidal solution is maintained by applying oscillation to the upper reservoir, an electric field is applied between the two reservoirs to make the colloidal particles move to the electroosmotic pump under the action of an electroosmotic flow, and a three-dimensional colloidal crystal is assembled; and the capillary is supplemented with an ultrasonic force to reduce lattice defects. The present application has the advantages of simple preparation process, controlled directional movement of colloidal particles through an electric field, improved assembly efficiency and quality of the three-dimensional colloidal crystal, and controllable assembly of the colloidal particles under the action of an electric field through regulation and control of the length and surface charge of the electroosmotic pump.

This invention discloses a complete set of reagents for detecting the interaction between post-translational modified proteins and their ligands. The complete set of reagents of this invention consists of four reagents: A, B, C, and D. A is composed of a biomolecule named R linked to a protein named X; B contains a biomolecule named L; R and L may be the same or different and interact with each other, with a phase transition occurring after the interaction between R and L; C is a polymer formed from a C monomer, wherein the C monomer consists of a monomer named mc, a reporter group named α, and a group named Y. C Molecules obtained by linking biomolecules, with two or more mc molecules, can form polymers; D is composed of molecules named X L The modified protein and its name is Y. D It is composed of biomolecules linked together; Y C With Y D There are interactions between them. This invention achieves high enrichment of interacting proteins and ligands in phase change droplets, amplifies weak interaction signals, and makes them easy to detect.

This invention provides a phenolic acid-modified attapulgite composite sunscreen agent, its preparation method, and applications, as well as a Pickering sunscreen lotion, belonging to the field of cosmetic technology. The method of this invention includes acid treatment of attapulgite, surfactant modification, loading of phenolic acid biomolecules, and hydrophobic modification. Using modified attapulgite as a carrier, this invention loads phenolic acid biomolecules through electrostatic adsorption to construct a composite synergistic system, and further modifies the surface with hydrophobicity to obtain a composite sunscreen agent with high stability and excellent sun protection performance. The sunscreen agent prepared by this invention combines physical shielding and biological protection functions, exhibiting excellent UV absorption capacity, good dispersibility, skin compatibility, and a pleasant feel on the skin. It solves the problems of nanoparticle aggregation, whitening, and poor photostability in existing sunscreen products, making it suitable for the cosmetic sunscreen field. It can achieve broad-spectrum UVA / UVB protection, antioxidant properties, and soothing repair, showing broad application prospects.

Described herein are methods and sensors for detecting a biomolecule analyte in a sample and uses related thereto. The sensor may utilise a substrate comprising a reference electrode, a counter electrode, and at least one working electrode; a sample activating composition in electrical contact with each of the electrodes and comprising a reducing agent and a complexing agent; and an electrical signal detector.

The present application relates to the technical field of starch coating, in particular to a preparation method of structured starch coating liquid with oil-proof and moisture-absorbing dual functions. The method comprises the following steps: firstly, performing mild acidolysis or selective oxidation pretreatment on cassava starch; then, preparing starch milk by using the pretreated starch, and performing synergistic enzymolysis on the starch milk under mild conditions by using a composite enzyme composed of alpha-amylase and glucoamylase in a specific ratio; after the enzymolysis, performing enzyme inactivation treatment, and then adding a bio-based crosslinking agent for compounding. The prepared coating has a specific bimodal distribution structure of starch derivative molecular weight, which is a key to realize function balance. After the coating liquid is coated on a substrate such as paper and dried and solidified, the formed coating not only has excellent oil-proof performance, but also exhibits good water vapor absorption capacity. The raw materials of the present application are green and the process is environmentally friendly, and the prepared multifunctional coating can be effectively applied to the fields of food packaging, medical protection and other fields which need to resist oil and regulate humidity at the same time.

The application discloses a molecular marker, a kit and a genotype detection method for predicting high content of highly unsaturated fatty acids in mirror carp muscle, relates to the technical field of biomolecular detection, and specifically relates to a molecular marker, a kit and a genotype detection method for predicting high content of highly unsaturated fatty acids in mirror carp muscle. The molecular marker for predicting high content of highly unsaturated fatty acids in mirror carp muscle is shown as SEQ ID NO. 1. The primer pair for predicting high content of highly unsaturated fatty acids in mirror carp muscle is hufaf1 and hufar1. The genotype detection method comprises the following steps: one, extracting DNA; two, PCR amplification; and three, Sanger sequencing, and the mirror carp with a C single peak at the 75th bp is selected. The application can identify individuals with potential high content of highly unsaturated fatty acids in muscle based on living body non-damage and rapidness through the above-mentioned molecular marker, the kit and the genotype detection method, so that precise and efficient breeding of the high content of highly unsaturated fatty acids in mirror carp is realized.

The present disclosure provides methods for detecting the presence or absence of cancer in a subject. In some embodiments, samples are obtained from the subject, and a panel capable of detecting multiple biological molecules, such as RNA, cDNA, and cell-free DNA (cfDNA) is utilized to processing a ample to determine tumor variants in the first sample. A mixture of these biological molecules are detected via oligonucleotide probes in order to detect as a particular sensitivity within a sequencing read budget, further including exon related probe designs for RNA.

This invention falls within the technical field of food, specifically relating to a process for obtaining a food product from the pods of Prosopis laevigata, resulting in a vegetable powder. The invention allows for obtaining a liquid phase containing the biomolecules present in the Prosopis laevigata pod and establishes the necessary conditions under which spray drying must be carried out, optimally transforming the liquid phase into a vegetable powder with food-grade qualities and quality. The resulting powder can be used as an important ingredient in the production of baking and confectionery products.

A biosensor for detecting a hormone is provided. The biosensor for detecting a hormone can include a core component and a biological molecule (such as a receptor for the hormone or an antibody against the hormone) immobilized on the biosensor. Also provided are methods for detecting or quantitating a hormone in a sample using the biosensor provided herein. The biosensor can be a bio-layer interferometry (BLI) biosensor, and the signals generated by binding of a hormone to the biosensor can be indicative of spectral shift, which can be measured using BLI. Additional signals such as UV absorbance of the hormone or signals of a detectable label may be measured. The biosensor can detect the presence or amount of the hormone in a sample accurately without interference by the presence of biotin, hemolysis, or lipid in a sample.

The application discloses a soybean plant height related InDel molecular marker and application thereof, relates to the technical field of biological molecule detection.The nucleotide sequence of the InDel molecular marker is shown as SEQ ID NO.4 or SEQ ID NO.5.The InDel molecular marker developed by the application can realize efficient, accurate and early identification of soybean plant height.The InDel molecular marker has stable polymorphism, high amplification efficiency of the matching specific primer, clear bands, good repeatability and clear typing.It is verified that the length difference of the amplification product is significantly related to the plant height phenotype, high-stalk and dwarf individuals can be accurately distinguished, and the genotype detection result is highly consistent with the field phenotype.The application provides a stable and reliable molecular tool for genetic improvement of soybean plant height and rapid cultivation of high-yield and lodging-resistant materials.

There is provided an atomic structure estimation apparatus for a biomolecule, including a generation unit generating a generation image based on a target biomolecule; an acquisition unit acquiring, from prepared atomic structures, first candidate structure images based on 2D projection images; a global search unit selecting, as a basic candidate structure, an atomic structure having a high degree of similarity to the target biomolecule in structure and / or molecular orientation, from among candidate structures of atomic structures, based on a degree of similarity between the generation image and a candidate structure image in the first candidate structure images; and a local search unit acquiring second candidate structure images based on 2D projection images of modified atomic structures modified from the basic candidate structure, and selects an atomic structure having a high degree of similarity to the target biomolecule in structure and / or molecular orientation, from among the modified atomic structures.

Compositions, systems, and methods for the display of analytes such as biomolecules are described. Display of analytes is achieved by coupling of the analytes to displaying molecules that are configured to associate with surfaces or interfaces. Arrays of analytes may be formed from the described systems for utilization in assays and other methods.

This item is a cassette primarily used for tangential flow filtration to filter fluids, for example, in the purification of biomolecules.

A method is provided for providing sustained, controlled, or prolonged release of a compound of interest, such as a biomolecule or drug. The method includes incubating the compound of interest together with mercerized decellularized cellulose particles for a predetermined incubation time to enable the mercerized decellularized cellulose particles to absorb the compound of interest. The method then includes modifying the physical or chemical properties of the decellularized particles to induce, alter, enable, or influence the sustained, controlled, or prolonged release of the compound of interest. Mercerized decellularized particles and formulations for sustained, controlled, or prolonged release of the compound of interest are also provided.

The application discloses taraxacum-derived carbon dots with tunable fluorescence and application thereof, and two kinds of carbon dots R-CDs and B-CDs with fluorescent colors can be obtained by high-temperature tuning with taraxacum as a carbon source, thereby creating conditions for double-channel fluorescence detection. The R-CDs can detect the percentage content of water in various organic solvents, and the test paper prepared can be repeatedly used for multiple times. The B-CDs are a multi-mode probe with fluorescence / colorimetric / absorption / RGB identification, and can simultaneously analyze and detect DNP in multiple ways. When a certain concentration of DNP is added into the B-CDs probe solution, a relatively significant fluorescence quenching phenomenon occurs, and the color of the solution becomes yellow. The specific detection of DNP is distinguished from other interfering components (metal ions, biological molecules and aromatic nitro compounds), and the detection method can be verified with each other, thereby improving the accuracy of detection. Meanwhile, the double-channel and multi-modal target detection is realized.

A polyacrylamide-based copolymer reduces or prevents aggregation of biologic molecules including proteins, peptides, and nucleic acids, and lipid-based vehicles such as liposomes, lipid nanoparticles, polymerosomes, and micelles, in aqueous formulations at hydrophobic interfaces, thereby increasing the thermal stability of the molecules in the formulation. Methods and compositions comprising the copolymer and a protein or the copolymer and insulin can be used for treating conditions including diabetes.

1. The name of the design product: colloidal gold immunochromatographic analyzer. 2. The use of the design product: for detecting biomolecules. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The application provides a coating and a preparation method thereof. The coating comprises a bismuth vanadate pigment modified by a plant polyphenol-protein complex, and the preparation method of the bismuth vanadate pigment comprises the following steps: A) mixing a plant polyphenol with a protein substance to obtain a complex modifier; B) mixing and stirring the complex modifier with a precursor solution containing bismuth nitrate and ammonium metavanadate to obtain a modified precursor; and C) calcining the precursor to obtain the bismuth vanadate pigment. The bismuth vanadate pigment modified by the plant polyphenol-protein complex provided by the application is applied to the coating, and by virtue of an innovative biomolecule synergistic modification technology, the acid and alkali resistance of the coating is significantly improved while the excellent chroma is maintained.

Provided herein are methods of determining molecular binding kinetics on particles, such as magnetic nanoparticles. In some embodiments, the methods include introducing an incident light from a light source toward a sample container that comprises a particle-bound biomolecule-ligand composition comprising a plurality of particle-bound biomolecules and a plurality of ligands that binds, or is capable of binding, to biomolecules of the plurality of particle-bound biomolecules, detecting light scattered from particle-bound biomolecule-ligand complexes in the particle-bound biomolecule-ligand composition over a duration to produce a set of imaging data using the detector, and determining size or volume changes of one or more of the particle-bound biomolecule-ligand complexes during at least a portion of the duration from the set of imaging data to thereby determine the molecular binding kinetics on the particles. Related systems and computer readable media are also provided.

The invention relates to the field of biomedicine, and provides an application of a Gram-negative bacterium infection target Kdo in research and development of antibacterial drugs. An authorized antibacterial peptide Ly (PEG)-1 is used as a probe; through computer simulation, affinity determination and gene defect strain identification, the key action target is lipopolysaccharide (LPS) internal core Kdo (3-deoxy-D-mannan-octanone acid), Kdo is used as a key component of bacteria LPS and is a basis for maintaining Gram-negative bacteria cell wall integrity, LPS biosynthesis and biological activity exertion, and the Kdo is used as a key component of bacteria LPS. The deletion or modification can directly cause the loss of LPS function and the reduction of the viability of bacteria. At present, no antibacterial drug aiming at the target spot exists, the problem that in the prior art, no biomolecule capable of targeting Kdo to exert strong antibacterial activity exists is solved, and the biomolecule can be used for developing drugs for diagnosing or treating related diseases.

The application relates to a target point recognition device, method, apparatus, computer readable storage medium and computer program product, relates to the technical field of biological information, and can improve the effectiveness of a target point recognition result. The target point recognition device is configured to perform the following steps: determining a candidate biological molecule in tumor cells that can provide an action target point for a targeted drug; performing expression level prediction on the candidate biological molecule by a trained expression prediction model, determining expression levels of the candidate biological molecule at multiple development stages of a tumor according to a prediction result; determining a first importance detection result of the candidate biological molecule in a tumor development process according to the expression levels of the candidate biological molecule at the multiple development stages of the tumor; and judging whether a drug action target point is determined according to the candidate biological molecule according to a tumor specificity detection result of the candidate biological molecule and the first importance detection result.

The application provides a beta'-thio-alpha, beta-unsaturated gamma-lactam derivative and a preparation method and application thereof, and belongs to the technical field of organic chemical synthesis. The beta'-thio-alpha, beta-unsaturated gamma-lactam derivative has a structure shown in formula I. The method constructs a beta'-thio-alpha, beta-unsaturated gamma-lactam derivative molecular skeleton through a free radical coupling and olefin isomerization process under the condition of blue light irradiation with cesium carbonate as an alkali. Through biological activity evaluation, it is found that the beta'-thio-alpha, beta-unsaturated gamma-lactam derivative prepared by the method has an inhibitory effect on MDA-MB-231 human breast cancer cells.

The application provides a Mach-Zehnder interference structure free-state molecule interaction analysis method and system, in a free system, a light intensity imaging module is used to perform molecule interaction monitoring, through a Mach-Zehnder interference optical path, a refractive index change caused by free system biomolecule combination is converted into a phase difference between two different polarization components formed by a detection light at a detection arm and a reference arm; subsequently, a weak value amplification effect is introduced by near-orthogonal post-selection, so that the small phase difference is converted into a light intensity change, and system noise interference is reduced through light intensity image processing, baseline correction and background subtraction; the application can solve the problems of spatial steric hindrance, limited mass transfer, complex detection process, difficulty in truly reflecting the molecule interaction process in the free system, insufficient sensitivity to weak signals, and easy environmental noise interference in the existing fixed system label-free detection technology, and can realize high-sensitivity, real-time and high-throughput detection of biomolecule interaction in a free solution system.

This invention discloses a magnetic microsphere modified with a high-density carboxyl polymer brush and its preparation method. The method includes the following steps: using ATRP technology, the polymerization of GMA is precisely initiated by an azide-based initiator to synthesize PGMA with controllable molecular weight and narrow distribution; the epoxy groups of PGMA undergo a ring-opening reaction with ethylenediamine, followed by reaction with bromoacetic acid to obtain a functionalized polymer brush; utilizing the high efficiency and specificity of azide-alkynyl click chemistry, the carboxylated polymer brush is covalently grafted onto the surface of the magnetic microspheres. This invention achieves precise design of the polymer brush molecular structure and successfully prepares magnetic microspheres with significantly increased carboxyl content, up to a maximum content of 1.495 mmol·g. ‑1 The polymer brush modification strategy of this invention significantly improves the functional group density on the surface of microspheres and their binding ability with biomolecules. The resulting magnetic microspheres have strong magnetic responsiveness and are not easily leaked. They can be used as high-performance platform materials and are widely used in fields such as bioseparation, diagnostic analysis and catalysis.

This item is a cassette primarily used for tangential flow filtration to filter fluids, for example, in the purification of biomolecules.

The application belongs to the technical field of biological medicine, and relates to a liver-targeting compound, a conjugate and application. The liver-targeting compound has a structure shown in formula I, T is a targeting group, B is a branch structure, L1 is a connecting group, L2 is a connecting part between the targeting group and the branch structure, and X is selected from integers between 2 and 6. The liver-targeting compound and the conjugate have clear structures, multiple galactose, galactosamine or galactosamine derivative molecules are linked into a three-cluster or four-cluster molecule to form a molecular cluster form, the affinity to ASGPR is obviously higher than the affinity of a single sugar, the drug liver-targeting delivery capacity can be improved, the delivery efficiency is high, and a long-acting inhibitory effect can be maintained; and the synthesis route is clear, and the preparation process is simple.

This application discloses a method for detecting CHI3L1 protein based on microfluidic fiber optic sensing, belonging to the field of biomedical detection technology. The method includes: providing a microstructured fiber optic sensing unit with a through-flow microfluidic channel; immobilizing a CHI3L1 protein-specific antibody on the inner surface of the microfluidic channel; injecting the sample to be tested into the channel, causing the CHI3L1 protein to be specifically captured; and achieving quantitative detection of the CHI3L1 protein by detecting the drift of fiber optic transmission spectral characteristic parameters caused by antigen-antibody binding. This invention combines microfluidics with fiber optic sensing, utilizing antibody immobilization on the inner wall of the microfluidic channel to increase the interaction efficiency between the light field and biomolecules, significantly improving detection sensitivity. It has the advantages of rapid detection, low sample consumption, and label-free operation, making it suitable for early non-invasive screening and dynamic monitoring of clinical liver fibrosis.

Methods and apparatus are provided for designing sequence-defined biomolecules, such as proteins, using a data-driven, evolutionary-based process. To design proteins, an iterative method based on a combination of unsupervised sequence-based models and supervised functionality-based models can be used to select candidate amino acid sequences that are likely to have the desired functionality. Feedback from measuring candidate proteins using a high-throughput gene synthesis and protein screening process is used to refine and improve the model, directing candidate selection toward the most promising regions of a very large amino acid sequence search space.