414 results about "Bio molecules" patented technology

The invention provides a beta '-sulfo-alpha, beta-unsaturated gamma-lactam derivative as well as a preparation method and application thereof, and belongs to the technical field of organic chemical synthesis. The beta '-thio-alpha, beta-unsaturated gamma-lactam derivative has a structure as shown in a formula I. According to the method disclosed by the invention, cesium carbonate is taken as alkali, and a molecular skeleton of the beta'-thio-alpha, beta-unsaturated gamma-lactam derivative is constructed through free radical coupling and olefin isomerization processes under the condition of blue light irradiation. Bioactivity evaluation shows that the beta '-sulfo-alpha, beta-unsaturated gamma-lactam derivative prepared by the method disclosed by the invention has an inhibition effect on MDA-MB-231 human breast cancer cells.

The invention provides a perovskite solar cell, a preparation method thereof and a photovoltaic module. The perovskite solar cell comprises a first electrode, a hole transport layer, a perovskite layer, an electron transport layer and a second electrode which are stacked in sequence. The material of the hole transport layer comprises a biomolecular material which is fixed on a self-assembly monomolecular layer material through an intermolecular force, and the biomolecular material comprises one or more of guanine, adenine, thymine, uracil, cytosine, hydroxymethylcytosine, xanthine, hypoxanthine, 6-mercaptopurine and corrin. According to the perovskite cell provided by the invention, the biomolecular material with a plurality of nitrogen heterocyclic structures and amino groups is added to generate multipoint hydrogen-bond interaction and pi-pi accumulation with the tail end of the SAM, so that the orderliness of molecular arrangement is improved, and the dipole direction and strength of the whole interface are regulated and controlled; and a coordination bond or hydrogen bond network is formed by the coordination compound and uncoordinated cations or anions in perovskite, so that interface defects are effectively passivated, and the thermal stability and the humid and hot life of a device are enhanced.

An evaporation device includes a sample fluid channel extending from a sample fluid inlet to a sample fluid outlet. The evaporation device is configured to evaporate a solvent of a sample fluid containing a target analyte to increase the concentration of the target analyte. The device includes a housing enclosing and defining a testing environment within which the evaporation device is disposed. The housing includes a heater, a temperature sensor, and a humidity sensor. A pump coupled to a gas inlet tube is configured to cause a gas flow within the testing environment adjacent to the evaporation device. A controller in communication with each of the heater, the temperature sensor, and the humidity sensor, is configured to activate the heater in response to at least one of a temperature value or a humidity value of the testing environment.

The invention discloses a rapid detection method for traumatic brain injury by fusing genetic engineering and quantum dots, and relates to the technical field of traumatic brain injury detection. The method comprises the following steps: acquiring a suspected patient biological sample, extracting biomolecule components, and comparing the biomolecule components with a healthy population biomolecule database to obtain related differential expression molecules. A specifically recognizable recombinant antibody is constructed by genetic engineering, and is connected with a quantum dot through a coupling reaction to prepare a detection probe. The probe and a biological sample are mixed and reacted, and the intensity of a fluorescence signal generated by combination is collected by a fluorescence detection device. A standardized signal value is obtained through signal processing algorithm noise reduction and feature extraction, and a preliminary detection result is determined by combining a bioinformatics database evaluation sample. And finally, according to the differential expression molecule, the standardized signal value and the preliminary result, analyzing a data change trend in a preset time window by using a multivariable statistical model, and generating a diagnosis report. According to the method, various technologies are fused, and a new path is provided for traumatic brain injury detection.

The invention discloses a pathogenic microorganism detection system based on nanopore sequencing and intelligent interpretation. The system comprises six modules including a biomolecule electric signal acquisition module and a pathogen characteristic sequence extraction module. Ion current change signals are collected through a nanopore chip array, a pathogen characteristic sequence is extracted through differential coding, a hypergraph topological structure self-adaption building module is used for building a pathogen relation model, a multi-dimensional heterogeneous pathogen parameter fusion analysis module integrates morphological parameters, metabonomics parameters and other multi-dimensional parameters, and the multi-dimensional heterogeneous pathogen parameter fusion analysis module is used for analyzing the multi-dimensional parameters. The optimized hypergraph neural network reasoning module realizes pathogen category prediction, and finally, the nanopore sequencing result intelligent interpretation output module generates a detection report containing pathogen categories, subtypes and risk levels. The system is combined with nanopore sequencing and an intelligent algorithm, pathogenic microorganism detection can be efficiently and accurately completed, the accuracy, reliability and efficiency of detection are greatly improved, and the requirement for rapid and accurate detection of pathogens in practical application is met.

The invention discloses a nanoparticle scattered light confocal imaging device and method. The device comprises a laser unit, a first pinhole, a beam splitter turntable, a scanning focusing unit, an electric objective table, a first detection imaging unit, a second pinhole, a light splitting unit and a second detection imaging unit. The method does not need to mark nano particles, and can synchronously collect unmarked nano particles and fluorescence-labeled biomolecules; in-situ imaging can be realized, and the distribution position and content of nano-particles in living cells can be directly observed; the imaging resolution ratio is high, single nanoparticle imaging can be carried out, and fluorescence imaging can also be carried out at the biomolecule level.

Provided herein are systems, devices, and methods for processing, analyzing, and classifying biological data sets and generation of cell profiles. The data sets may include multi-omic data. Some embodiments may include the use of machine learning in training a classifier of raw multi-omic data and incorporating system biology knowledge to understand cellular behavior and cell status at the biomolecular level.

The invention discloses a reduced graphene oxide biochip based on a piezoelectric substrate and a preparation method and application thereof, the reduced graphene oxide biochip based on the piezoelectric substrate comprises a sound transduction unit and at least one detection unit connected to the sound transduction unit, and a microfluid channel layer is arranged between the sound transduction unit and the detection unit. A reduced graphene oxide layer is directly formed on the surface of a piezoelectric layer of a detection unit, a biological recognition element is fixed on the reduced graphene oxide layer, and the biological recognition element is used for being combined with target biological molecules to generate a reaction product with electron donating and receiving capacity. On the basis, the acoustic transduction electrode design of a concentric interdigital electrode structure is adopted, and the acoustic surface wave intensity which is more concentrated compared with other electrode structures is generated by utilizing the focusing effect of the concentric electrode structure; the flow velocity of the fluid to be measured in the microfluid channel is faster, and the directivity is stronger and more concentrated.

Provided is a temperature-responsive fluorescent particle comprising at least one type of fluorescent molecule in a molecular assembly comprising and constituted by at least one type of amphiphilic molecule, wherein the fluorescent molecule emits fluorescence when the molecular assembly is in the liquid phase and it is quenched when the molecular assembly is in the solid phase, due to a temperature-responsive solid-liquid phase transition, so that fluorescence emission and quenching of the fluorescent molecule are reversibly switched in a temperature responsive manner. Also provided is a temperature-responsive fluorescent probe comprising the temperature-responsive fluorescent particle the surface of which is modified with a biomolecule recognition element, and methods for detecting and quantitatively determining a biomolecule with the temperature-responsive fluorescent probe.

The invention relates to RNA constructs encoding (i) at least one therapeutic biomolecule; and (ii) at least one innate inhibitor protein (IIP). The constructs are RNA replicons and saRNA molecules, and the invention includes genetic constructs or vectors encoding such RNA replicons. The invention extends to the use of such RNA constructs and replicons in therapy, for example in treating diseases and / or in vaccine delivery. The invention extends to pharmaceutical compositions comprising such RNA constructs, and methods and uses thereof.

The present application relates to a controllable preparation method of a colloidal crystal capillary column and its application in biomolecule separation, and specifically comprises the following steps: a plunger and an electroosmotic pump are prepared in situ at one end of a capillary; a buffer solution containing monodisperse colloidal particles is filled in an upper reservoir of an electric field assembly device, and a buffer solution is filled in a lower reservoir; the capillary is filled with the buffer solution, and the end of the capillary containing the electroosmotic pump is inserted into the lower reservoir; the dispersion of the colloidal solution is maintained by applying oscillation to the upper reservoir, an electric field is applied between the two reservoirs to make the colloidal particles move to the electroosmotic pump under the action of an electroosmotic flow, and a three-dimensional colloidal crystal is assembled; and the capillary is supplemented with an ultrasonic force to reduce lattice defects. The present application has the advantages of simple preparation process, controlled directional movement of colloidal particles through an electric field, improved assembly efficiency and quality of the three-dimensional colloidal crystal, and controllable assembly of the colloidal particles under the action of an electric field through regulation and control of the length and surface charge of the electroosmotic pump.

The invention provides a surface plasma imaging method and a surface plasma imaging device, which are used for imaging surface plasma signals caused by interaction of biomolecules. More specifically, the invention provides a method and a device capable of imaging a surface plasmon resonance mode in a frequency domain through tiny change by comparing the light intensity in two sections of surface plasmon frequency domains.

A polypeptide linker (2 to 21 amino acids) is connected to a carrier peptide based on the Ioop2 domain of lynx1. The carrier or transporter peptide may be further coupled via the polypeptide linker to a molecule of interest (MOI) or effector agent, such as a protein or protein domain or any other biomolecule (e.g., biomolecules having a molecular weight of 10kDa to 230 kDa). In some embodiments, the complex may be used therapeutically in targeting various neurological disease-causing cellular components.

The present invention relates to methods and devices for isolating one or more biomolecules of interest from a biological composition. Specifically, the invention relates to the separation of one or more target biomolecules from a biological composition by dynamic packed bed adsorption using a separation medium comprising porous rod-like particles attached to the target molecules and filtration using a filter having a pore size of at least 1.5 [mu] m.

The invention provides an S100B and NSE double-signal electrochemical immunosensor, a preparation method, application and a detection product, and belongs to the technical field of electrochemical detection. According to the preparation method, the PAMAM dendritic polymer rich in amine-terminated functional groups is covalently grafted to the MXene conductive sheet layer with a large surface area to prepare the PAMAM-MXene composite material, so that the immobilization efficiency and interface electron transfer of immune molecules are remarkably improved through modification, and enhanced conductivity and relatively high specific surface area are shown; and the biomolecule loading capacity and the signal transmission efficiency of the electrode are also remarkably improved, and double-signal detection can be performed on S100B and NSE at the same time. And the electrochemical immunosensor has good reproducibility, long-term stability and anti-interference capability on the two biomarkers. According to the sensor, modular design and signal decoupling are adopted, double targets can be accurately detected in parallel, and an extensible strategy is provided for multiplexing biomarker analysis.

Provided herein is a method of delivering a biological material, including a genome-editing agent, to the interior of a plant cell wherein the method includes contacting the plant cell with a complex comprising the biological material and a fluorous agent. Also provided herein are compositions and reagents for practicing the method.

The invention belongs to the technical field of liquid crystal laser sensing, and relates to an estradiol detection device based on a liquid crystal-gold nanoparticle laser sensor. The optical flow control optical fiber is used as an echo wall resonator. In order to realize an estradiol sensing function, the DNA aptamer is modified on the inner surface of the optical flow control optical fiber to realize specific capture of estradiol. After the aptamer is combined with estradiol, the interaction between the aptamer and estradiol triggers the aptamer imaging change, so that the liquid crystal molecule realizes the conversion from a bipolar type to a radial type, and the conversion leads to the reduction of the refractive index, thereby causing the blue shift of the spectrum. By virtue of a local disturbance effect based on the gold nanoparticles, a quadruple synergistic effect of hyperpolarizability change of modified biomolecules, liquid crystal molecule orientation and echo wall mode resonance, an opportunity is provided for sensing and amplifying subtle detection of a target binding event. The problem of low sensitivity caused by common naked eye observation or wide spectrum monitoring in a liquid crystal microdroplet sensor is solved.

The invention relates to the technical field of biomolecular markers, and provides a chicken butyric acid level and / or death rate related SNP marker and application thereof, the SNP marker comprises a 106476774th nucleotide site corresponding to a No.3 chromosome of a GRCg6a-galGal6 genome, and the basic group of the nucleotide site is A or T. The invention also provides an application of the SNP marker in chicken butyric acid level and / or death rate related SNP marker in chicken butyric acid level and / or death rate related SNP marker in chicken butyric acid level and / or death rate related SNP marker in chicken butyric acid level and / or death rate related SNP marker. By optimizing the dominant alleles of the SNP, broilers with the butyric acid level and / or high and low mortality can be rapidly identified, broilers with high mortality can be eliminated, the frequency of the dominant alleles is increased generation by generation through breeding, the butyric acid level and / or mortality of the broilers, especially the 28-42-day-old stage, of the broilers is reduced, the genetic improvement progress of the domestic broilers is accelerated, and the breeding efficiency of the domestic broilers is improved. Therefore, the economic benefit of chicken breeding is effectively improved, and the application value is high.

The invention provides a graphene transistor sensor and a preparation method and application thereof, and belongs to the technical field of biosensors. The graphene transistor sensor is used for noninvasive detection of depression and can detect depression-related markers in body fluids such as sweat, urine and saliva through noninvasive collection. According to the invention, a biosensing chip is constructed by using a graphene transistor array modified by gold nanoparticles, and simultaneous monitoring of multiple biomarkers can be realized by fixing different aptamers; the response speed and the sensitivity of the sensor are improved by utilizing the ultrahigh carrier mobility of the single-layer graphene with the quasi-single-crystal structure, and high-precision accurate detection is realized when the concentration of a biomarker is relatively low; the gold nanoparticle layer is used for fixing the aptamer of the target biomolecule, the coverage rate of the gold nanoparticles can be regulated and controlled by limiting the thickness of the gold film, more active sites are provided, the detection range can be expanded, and the detection requirement for continuously and dynamically monitoring the concentration change of the biomarker is met.

The invention discloses a molecular marker, a kit and a genotype detection method for predicting high content of highly unsaturated fatty acid in mirror carp muscle, and relates to the technical field of biomolecular detection, in particular to a molecular marker, a kit and a genotype detection method for predicting high content of highly unsaturated fatty acid in mirror carp muscle. The molecular marker for predicting the high HUFA content of the mirror carp muscle is as shown in SEQ ID NO. 1. The primer pair for predicting the high HUFA content of the mirror carp muscle is hufaf1 and hufar1. The genotype detection method comprises the following steps: 1, extracting DNA; 2, carrying out PCR (Polymerase Chain Reaction) amplification; 3, Sanger sequencing is carried out, and the mirror carp with a C single peak appearing at the 75bp position is selected. By means of the molecular marker, the kit and the genotype detection method, individuals with muscle high HUFA content potential can be rapidly identified based on living body losslessness, and therefore accurate and efficient breeding of the mirror carp high HUFA content character is achieved.

The invention belongs to the technical field of oilfield chemistry, and discloses a preparation method of an efficient nano-enhanced oilfield fracturing fluid, which remarkably improves the performance, environmental protection property and high efficiency of the fracturing fluid through the combination of a self-adjusting mechanism of self-assembled nanoparticles and an enzyme-nanotechnology. The self-assembled nano-particles can automatically adjust the form or the structure when the external environment changes, so that the rheological property and the sand-carrying capacity of the fracturing fluid are optimized, and enzyme is introduced as a biomolecule to be combined with the nano-particles, so that the fracturing fluid has self-healing and degradation functions, the environmental protection property and the adaptability of the fracturing fluid are effectively improved, and in the oil field fracturing process, the fracturing fluid has the advantages that the self-healing and sand-carrying capacity is greatly improved. The composite material not only can enhance the performance of the liquid, but also can reduce the pollution to the environment, and meets the requirement of sustainable development.

The invention provides a molecular interaction network protection system based on structural biology and an optimization method thereof, and relates to the technical field of biotechnology and molecular engineering.The molecular interaction network protection system comprises a USAG-1 protein binding domain, and the USAG-1 protein binding domain comprises a Glu32-Arg58-Ser76 hydrogen bond network and a Phe41 / Trp65 / Leu83 hydrophobic core; the MMP-9 cleavage site mutant is used for carrying out 20 substitutions from D to N / V / A in the PLGLA sequence, and is combined with G52S mutation; the ellipticity of the CD spectrum of the RGD peptide at 222nm is less than or equal to-15 mdeg, and the RGD peptide comprises a salt bridge at the i / i + 4 position. The USAG-1 protein binding domain also comprises an Asn45 carbohydrate chain, and the molecular weight of the Asn45 carbohydrate chain is 500 to 2000 Da. The phi angle of the RGD peptide is-57 + / -5 degrees, and the psi angle of the RGD peptide is-47 + / -5 degrees. According to the invention, through a multi-dimensional structure locking strategy, while the functional activity of biomolecules is maintained, accurate control of alpha-helical conformation is realized, a resistance mutation library is constructed, and the fundamental contradiction of stability-activity imbalance, conformation control misalignment and single-point mutation drug resistance in the traditional technology is thoroughly solved.

This invention discloses a complete set of reagents for detecting the interaction between post-translational modified proteins and their ligands. The complete set of reagents of this invention consists of four reagents: A, B, C, and D. A is composed of a biomolecule named R linked to a protein named X; B contains a biomolecule named L; R and L may be the same or different and interact with each other, with a phase transition occurring after the interaction between R and L; C is a polymer formed from a C monomer, wherein the C monomer consists of a monomer named mc, a reporter group named α, and a group named Y. C Molecules obtained by linking biomolecules, with two or more mc molecules, can form polymers; D is composed of molecules named X L The modified protein and its name is Y. D It is composed of biomolecules linked together; Y C With Y D There are interactions between them. This invention achieves high enrichment of interacting proteins and ligands in phase change droplets, amplifies weak interaction signals, and makes them easy to detect.

The invention provides an application of N-(3-cyano-tetrahydro-1-benzothiophene-2-yl)-2-(morpholine-4-yl) acetamide in the treatment of an Alzheimer's disease. The CN-0928 compound and the analogue CN-0929 of the CN-0928 compound can be used for reducing BACE1 protein expression by regulating and controlling a biomolecular aggregate, so that the A beta plaque load is reduced; in addition, mitochondrial structure change can be recovered, so that AD pathology is further relieved. The treatment based on the intracellular RNPs particles provides a new thought for developing drugs for preventing or treating neurological diseases such as Alzheimer's disease and the like.

The present invention provides a recombinant immune cell and the preparation method, the gene regulation system and the use thereof. By reducing or eliminating the expression and / or biological functions thereof of the BCOR gene and the ZC3H12A gene, the persistence of the recombinant immune cell is enhanced. In some embodiments, the present invention obtains CAR-T cells with knockout of double genes ZC3H12A and BCOR by gene editing, which can persist in vivo, solving the technical problem of long-term effectiveness of CAR-T treatment. In some embodiments, the gene-edited CAR-T cells persist in vivo and can continuously secrete therapeutic biological molecules, achieving the purpose of long-term effectiveness of a single administration.

The application relates to the technical field of semiconductor devices, and discloses a biosensor based on a parallel tunneling junction vertical tunneling field effect transistor and a preparation method thereof. The sensor comprises a stepped drain region, drain contacts arranged on the two sides of the drain region, a stepped channel layer arranged on the top of the drain region, source regions arranged on the two sides of the channel layer, source contacts arranged on the top of each source region, a groove arranged on the top layer of the channel layer, a gate dielectric layer arranged in the groove, a gate metal arranged on the upper surface of the gate dielectric layer at the bottom of the groove, and a cavity formed between the gate metal and the gate dielectric layer, which is a biomolecule detection cavity. The preparation method comprises the following steps: etching the drain region and the channel layer to obtain a target shape and a groove structure, embedding the source region and an oxide layer, etching part of the oxide layer and embedding the gate metal, etching the oxide layer on the two sides of the gate metal and growing the gate dielectric layer, forming the biomolecule detection cavity, and metalizing part positions of the source region and the drain region to obtain a final structure. The device current and the sensitivity of biomolecule detection are improved.

The invention discloses a biomolecular interaction prediction method based on multi-modal attention fusion, and belongs to the technical field of artificial intelligence drug discovery. The method comprises the following steps: acquiring multi-modal characteristics of drugs, targets, diseases and genes: sequence structure characteristics, 3D structure characteristics, similarity network characteristics and biological relation network embedding characteristics; constructing a feature fusion prediction model, and performing training; inputting the multi-modal features of the two biological entities into the trained feature fusion prediction model, and outputting the probability of interaction of the two biological entities; the feature fusion prediction model comprises a Transform encoder and an MLP (Markup Language Protocol) network; the multi-modal features are input into a feature fusion prediction model for stacking and then are input into a Transform encoder, the features are processed by using a multi-head self-attention mechanism, and an output result is flattened and subjected to dimension reduction processing to obtain embedded vector representation; and finally, performing element corresponding multiplication on the embedded vectors of the two biological entities, inputting the embedded vectors into an MLP network, and outputting an interaction probability between the two biological entities.

The application discloses a preparation method of nano copper powder and belongs to the technical field of nano copper powder preparation. The method comprises the following steps: S1, preparing a mercaptopropionic acid-ethanol solution; S2, preparing surface carboxylated quantum dots; S3, preparing an enzyme solution; S4, preparing an enzyme solution in which surface carboxyl groups are converted into active esters; S5, preparing a surface carboxylated quantum dot solution; S6, preparing a quantum dot-biomolecule mixture; S7, preparing a copper salt solution and adjusting the pH to 5-6; and S8, preparing nano copper powder. In the method, copper ions in the copper salt solution are reduced into copper atoms by the quantum dot-biomolecule mixture to prepare the nano copper powder. The prepared nano copper powder can accurately control the nucleation rate and quantity of the nano copper powder, and has low preparation cost and high preparation efficiency.

The invention relates to a sampler for collecting biological molecules on the surface of biological tissue, which comprises a main body provided with a power device, and the sampler is driven by the power device to rotate; the sampling units are provided with braking structures and are connected with the main body through the braking structures; wherein the braking structures are arranged in the following manner: when the number of the braking structures is less than or equal to 2, the braking structures are set as automatic adjusting parts and are used for automatically adjusting the position of the sampling unit so as to adjust the sampling radius; when the number of the braking structures is larger than 2, the braking structures are set to be mechanical adjustable structures, and the positions of the sampling units are preset and fixed through the braking structures before use, so that the sampling radius is preset and fixed. The system has the characteristics of adjustable resolution and soft contact, disturbance to tissues is reduced through a non-embedded biomolecule sampling technology, tissue full-spectrum characterization can be completed in combination with pre-planned complete sampling motion route design, and finally biomolecule collection is achieved.

The present disclosure describes PLPC-DB, an ultrapure phospholipoproteomic composition consisting of essential phospholipids, bioactive proteins, and intercellular regulatory factors, derived from the supernatant of peripheral blood mononuclear cells (PBMCs). This composition achieves a purity level exceeding 99% through a patented purification process that integrates high-speed advanced centrifugation and selective ultrafiltration, ensuring the structural stability and functional integrity of its bioactive components. PLPC-DB is optimized for advanced research and diagnostic applications, providing reproducibility, consistency, and safety across multicenter studies. The essential biomolecular components of PLPC-DB include phosphatidylcholine and phosphatidylserine, which contribute to membrane stability and intracellular signaling, while cell communication peptides enhance intercellular signaling, homeostatic regulation, and biochemical coordination. Structural and regulatory lipids support cell membrane biogenesis and functional stability, whereas adhesion and signaling proteins mediate cell-cell interactions and immune response coordination. Additionally, bioactive regulatory factors modulate immune and inflammatory responses, contributing to tissue regeneration and metabolic homeostasis.