1547 results about "Biomolecule" patented technology

The embodiments of the present disclosure relate to decreasing the bioavailability of one or more target biomolecules by providing a composition that comprises a recombinant plasmid with one or more sequences of micro interfering ribonucleic acid (miRNA). When the recombinant plasmid interacts with a target cell, it causes the target cell to upregulate production of the miRNA, which then decreases the bioavailability of the target biomolecule. In some embodiments of the present disclosure, the target biomolecule is a cytokine or other mediator molecule of an inflammatory response.

The embodiments of the present disclosure relate to one or more compositions or methods that upregulate the production of one or more sequences of micro-interfering ribonucleic acid (miRNA). The sequences of miRNA may be complimentary to a sequence of target messenger RNA (mRNA) that encodes for translation of a target biomolecule, such as JAK. The miRNA can cause the target mRNA to be degraded or inactivated, thereby causing a decrease in bioavailability of the target biomolecule because it is degraded or inactivated by the miRNA. Decreasing the bioavailability of the target biomolecule within a subject that is administered the one or more compositions may address the afflictions experienced by the subject due to expression of the target biomolecule.

The present disclosure relates to compositions and methods for reversible fixation of biological samples using fixation reagents that form bis-carbamate crosslinks between amine-bearing moieties in biomolecules.

Provided herein are systems and methods for analyzing biomolecules (e.g., nucleic acid molecules, proteins). A method of nucleic acid analysis can comprise: (a) providing a sample comprising a cell comprising a target polynucleotide comprising a first exon segment and a second exon segment, wherein the first exon segment and the second exon segment flank opposite ends of a splice junction site of the target polynucleotide. The method can further comprise (b) contacting the cell with: (i) a first probe, wherein the first probe hybridizes to a first target sequence of the first exon segment, and (ii) a second probe, wherein the second probe hybridizes to a second target sequence of the second exon segment. The method can further comprise (c) linking the first probe and the second probe together, thereby generating a probe-linked nucleic acid molecule comprising the first probe and the second probe. The method can further comprise (d) identifying a sequence of the probe-linked nucleic acid molecule or derivative thereof, thereby locating the splice junction site of the target polynucleotide.

The embodiments of the present disclosure relate to one or more compositions or methods that upregulate the production of one or more sequences of micro interfering ribonucleic acid (miRNA). The sequences of miRNA may be complimentary to a sequence of target messenger RNA (mRNA) that encodes for translation of a target biomolecule, such as ETS2. The miRNA can cause the target mRNA to be degraded or inactivated, thereby causing a decrease in bioavailability of the target biomolecule because it is degraded or inactivated by the miRNA. Decreasing the bioavailability of the target biomolecule within a subject that is administered the one or more compositions may address the afflictions experienced by the subject due to overexpression of the target biomolecule.

A ternary deep eutectic solvent (TDES) system for a biomolecule (for example, chitin) recovery from a biomass (for example, seafood waste), and the method of preparation of ternary deep eutectic solvent (TDES) system, and a process to differentiate / extract / recover biomolecules (such as chitin) from a biomass (such as seafood waste).

Systems and methods are provided for stabilization of cells and retention of biomolecules therein for further analysis. Block copolymers are inserted into membranes of biological particles, cell membranes or organelles (e.g., organelle membranes) and polymerized to form an intra-biological particle (e.g., intracellular) polymer network.

The invention provides a biological microenvironment image analysis and classification method, which relates to the technical field of cell image analysis and comprises the following steps: processing an input biological microenvironment image, segmenting cell components and extracting multi-parameter initial features of the cell components; a first machine learning model is adopted to deduce a refined functional sub-state exceeding a traditional cell type based on the internal characteristics and local microenvironment information of the cells; in combination with biomolecule interaction knowledge, calculating and generating a spatially resolved biomolecule interaction potential field map so as to quantify intercellular communication potential; forming a comprehensive state descriptor set; and inputting the descriptor set into a second machine learning classification model to generate a precise classification result of the biological microenvironment. According to the method, functional, quantitative and interpretable analysis and classification of the biological microenvironment are realized, and the evaluation depth, objectivity and accuracy are remarkably improved.

A platform for characterizing biomolecules, biological tissue sections, viruses, or other particles of interest in a non-invasive, high-throughput, cost-effective, and label-free manner. The platform includes a merged set of imaging modalities including and capable of interferometric microscopy and wide-field or confocal microscopy (such as, for example, a wide-field super-resolution surface enhanced Raman spectroscopy, SERS), although some aspects may be employed with data sets only received from wide-field or confocal microscopy. Related devices, systems, methods, techniques, and variations to the same are also provided.

A hydrogel particle, comprising a matrix comprising a polymerized monomer, said matrix comprising a plurality of micropores and a plurality of macropores, and one or more immunostimulatory biomolecules selected from the group consisting of an anti-CD3 antibody or antigen-binding fragment thereof, an anti-CD28 antibody or antigen-binding fragment thereof, and combinations thereof.

This application discloses a rapid detection method for screening of dwarfism in children, which belongs to the field of intelligent medical technology. It includes the following steps: Step 1, accurately sampling through capillary effect, and automatically diluting the sample to ensure accurate concentration; Step 2, introducing the collected sample into a multi-channel microfluidic detection chip to achieve parallel quantitative detection of endocrine hormones; Step 3, real-time acquisition and amplification of weak biomolecule recognition signals to effectively improve the signal-to-noise ratio; Step 4, multi-dimensional analysis of the test results to achieve quantitative evaluation of growth and development, body mass index and sexual development indicators and risk prediction of endocrine diseases; Step 5, automatically integrating the analysis results and generating a standardized test report; Step 6, establishing a quality control mechanism throughout the entire detection process, including real-time monitoring of sample quality, tracking of detection process parameters, result consistency evaluation, and full traceability of detection data.

The present disclosure relates to compositions and methods for reversible fixation of biological samples using fixation reagents that form bis-carbamate crosslinks between amine-bearing moieties in biomolecules.

The present disclosure relates HER2 antibodies and to the de novo design of structure-based design targeted antibodies. According to the current disclosure, computer-implemented methods of designing an antibody sequences that will bind the HER2 with significant affinity at a target receptor epitope are disclosed. The method utilizes artificial intelligence (Al) and machine learning (ML) models that are trained to predict improved biomolecule (e.g., antibody or antibody fragments) binding sequences or other desirable properties using known biomolecule / binding partner complexes. A designed HER2 antibody is generated by replacing matching residues with different residues such that a predicted affinity is increased.

The invention discloses a non-coding RNA (Ribonucleic Acid) and disease association prediction method based on a graph neural network, and belongs to a biomolecule association prediction technology in the field of biological information. The invention breaks through the limitation of a single task of a conventional method, and provides a novel multi-task learning framework based on task migration. Potential LDA, MDA and LMI can be deduced by effectively utilizing the known complex relationship among lncRNA, miRNA and diseases. A plurality of graph structures are constructed, including a bipartite graph, a feature structure graph and a meta-path graph. The synergistic effect of the multi-graph structure significantly enriches the context information of the nodes, and effectively improves the prediction accuracy of the model. Pairwise attribute learning is introduced and is used for capturing the direct relationship among lncRNA, miRNA and diseases. According to the method, the prediction performance is improved, overfitting is effectively reduced, and the robust generalization ability of the model is ensured.

Strategies, systems, methods, reagents, and kits for phage-assisted continuous evolution are provided herein. These include strategies, systems, methods, reagents, and kits allowing for stringency modulation to evolve weakly active or inactive biomolecule variants, negative selection of undesired properties, and / or positive selection of desired properties.

The invention discloses a kit for detecting salmonella enteritidis and application of the kit, and belongs to the technical field of biomolecular detection. The kit comprises the following components: an allosteric aptamer probe of which the nucleotide sequence is as shown in SEQ ID NO.1, a template chain of which the nucleotide sequence is as shown in SEQ ID NO.3 and a fluorescence report probe of which the nucleotide sequence is as shown in SEQ ID NO.4. According to the kit, salmonella enteritidis can be distinguished from other impurities, and the specificity is high; a visual fluorescence signal can be obtained and is easy to interpret; according to the invention, a template chain can be cyclically utilized in the reaction to generate a Trigger chain to guide the TtAgo protein to efficiently and specifically cut a fluorescent reporter probe, the output signal is increased, the detection time is short, the detection limit is low, the sensitivity is high, the specificity is good, and the method has practical popularization and application prospects.

Various implementations, systems and methods are disclosed for continuous, near real-time, hands-off sampling of airborne particulate matter, and qualification and / or quantification of biomolecules in the sample representative for biologic or microbial contamination. The systems and methods may utilize an electrostatic precipitator for sampling the matter; and a measurement assembly configured to illuminate, excite, or breakdown the sampled matter by electromagnetic radiation, and to detect a spectrum, or one or more wavelength bands of the scatter emitted by the sample. In an exemplary implementation, a sputter deposition process is employed to configure the sample for an enhanced plasmon resonance. The measurement data may be transferred via wireless communication means for cloud storage and signal processing.

The invention discloses a multi-active targeting tumor nano-drug with a multimode imaging function and an immune activation effect and a preparation method thereof, and belongs to the technical field of nano-drugs. The preparation method comprises the following steps: coupling inorganic metal-based nano particles / nano drugs with a multimode imaging function or / and targeting active organic biomolecules with specific functions and specific therapeutic drugs through a silane cross-linking agent or a combination of the silane cross-linking agent and a carbodiimide condensing agent; the active targeting type organic-inorganic composite nano-drug aggregate with a multi-mode imaging function and an immune activation effect is prepared. Wherein the inorganic component is a metal-based heterostructure nanoparticle with a multimode imaging function, and the organic component is a bioactive molecule or / and a specific therapeutic drug. The obtained nano-drug aggregate is stable in system, uniform and controllable in size and good in biocompatibility, and has specific targeting, such as specific targeting liver cancer cells.

A method of providing a slow, controlled or sustained release of a compound of interest such as biomolecules or drugs is provided. The method involves incubating the compound of interest with mercerized decellularized cellulose particles for a predetermined amount of incubation time to allow the mercerized decellularized cellulose particles to absorb the compound of interest. Thereafter, the method involves adjusting a physical or chemical property of the decellularized particles to trigger, alter, enable or affect the slow, controlled or sustained release of the compound of interest. Mercerized decellularized particles and formulations for slow, controlled or sustained release of compounds of interest are also provided.

The invention discloses a preparation method of a protein halo-coated silicon-coated gold nanorod and a PCR amplification method and kit based on the protein halo-coated silicon-coated gold nanorod, and belongs to the technical field of PCR detection. The photo-thermal nano material is composed of an AuNR coated SiO2 core-shell structure and an external protein halo layer, the protein halo layer avoids inhibition of AuNR on a PCR reaction system, meanwhile, adsorption of AuNR on biological small molecules is effectively reduced, non-specific amplification is reduced, and normal amplification of PCR reaction is guaranteed; and the protein halo layer is used as a thermal insulation layer to optimize heat exchange and maintain the temperature uniformity of the reaction liquid. When the kit is applied to a photo-thermal PCR detection reagent, DNA of a pathogen to be detected can be efficiently and stably amplified and detected within 10 minutes, the kit has huge application potential in the fields of early screening of infectious diseases, food safety detection and the like, collaborative optimization of photo-thermal conversion efficiency, biocompatibility, detection sensitivity and operation convenience is achieved, and the kit has a wide application prospect. And an efficient solution is provided for prevention and control of infectious diseases, food safety monitoring and biomedical research.

Disclosed are a nanopore-optical electronic device and a method for sequencing a biomolecule and modifications thereof. The nanopore-optical electronic device includes: a cis-fluidic chamber and a trans-fluidic chamber fabricated in a planar substrate; a nano-fluidic channel in between and connecting the cis-fluidic chamber and the trans-fluidic chamber; a first electrode and a second electrode sealed in the nano-fluidic channel and forming a nanogap / nanopore therebetween; a third electrode and fourth electrode disposed in the cis-fluidic chamber and the trans-fluidic chamber, respectively; an optical coupling element coupling an electromagnetic beam with the nanogap; an optical detector detecting an optical signal from the nanogap when a biomolecule translocates through the nanopore; and a current-measuring circuit concurrently measuring a tunneling current and ionic current when the biomolecule translocates through the nanopore. The recorded optical signal and tunneling current and ionic current signals are coincidental in time and colocalized in space.

The invention discloses a lipid compound capable of being used for gene delivery, a preparation method of the lipid compound and application of the lipid compound in gene delivery. Also disclosed herein is a lipid nanoparticle comprising the lipid compound, a gene delivery composition comprising the lipid compound or the lipid nanoparticle. The lipid compounds, lipid nanoparticles and delivery systems herein enable efficient complexation, protection, intracellular and targeted delivery and release of biomolecules, such as oligonucleotides and nucleic acids, in vitro and in vivo tissues and organs.

The invention provides a wide pH applicable multi-biomolecule detection MOF probe, a preparation method, a detection method and application, the probe is an MIL-53 (Fe) (at) AA (at) EZ probe, and comprises a metal organic framework MIL-53 (Fe), amino acid and enzyme; the preparation method comprises the following steps: weighing terephthalic acid and ferric chloride hexahydrate, adding into N, N-dimethylformamide, mixing and stirring, pouring the mixed solution into a stainless steel autoclave, heating and reacting, naturally cooling to room temperature, centrifugally separating a product, and washing with N, N-dimethylformamide and absolute ethyl alcohol to obtain the metal organic framework MIL-53 (Fe). The preparation method comprises the following steps: mixing a metal organic framework MIL-53 (Fe) with an amino acid solution, and carrying out ultrasonic treatment and stirring to obtain an MIL-53 (Fe) coated AA solution; the method comprises the following steps: dissolving enzyme in an MIL-53 (Fe) (at) AA solution, uniformly mixing, stirring, centrifugally separating a product, taking precipitate, and drying in vacuum to obtain the MIL-53 (Fe) (at) AA (at) EZ probe. The pH application range is wide, one-step detection can be achieved, and the method has the advantages of being rapid in reaction, high in sensitivity, low in detection limit and easy and convenient to operate.

The embodiment of the invention discloses a terahertz virus spectrum classification method and system based on transfer learning, and the method comprises the steps: obtaining a source domain terahertz spectrum data set containing various biomolecule hydrogen bond vibrations, and obtaining an initial feature extraction model; extracting a hydrogen bond vibration characteristic mode in a preset frequency band range, and taking a characteristic center of the hydrogen bond vibration characteristic mode as a cross-domain bridging characteristic; obtaining target sample data, wherein the sample data is a small sample data set; converting the similarity between the feature center and a target sample spectrum peak into a dynamic weight of a migration loss function, and performing layer-by-layer fine adjustment on the initial feature extraction model network to obtain a virus classification model; the fine tuning process comprises the following steps: adjusting a weight factor in real time according to the spectrum peak offset of the current batch of samples; inputting the terahertz spectrum data of the to-be-detected virus into the virus classification model to obtain a classification result of the to-be-detected virus. According to the application, the accuracy, robustness and industrial applicability of terahertz virus detection are remarkably improved.

This invention is related to the field of biosensors. In particular, devices and methods are described which enable the quantification of biomolecules by virtue of particle speed driven by induced-charge electrophoresis. For example, a range of biomolecules can be simultaneously detected in a highly sensitive manner from a library (e.g., combination) of active particles having different shapes or fluorescent signatures. Such biomolecules are related to conditions including, but not limited to, cancer biomarkers, viral infection biomarkers, toxins, pesticides, and small molecule drugs.

The invention belongs to the technical field of electrochemical sensors, and particularly relates to a cobalt-based modified industrial solid waste vinegar residue derived carbon composite material and a preparation method and application thereof. The preparation method comprises the following steps: washing, drying, crushing and sieving industrial waste vinegar residues to obtain vinegar residue powder; carbonizing the vinegar residue powder in an inert atmosphere to obtain carbonized vinegar residues; the carbonized vinegar residues are mixed in a hot alkali solution for activating treatment, and then Co3O4 is derived through inorganic cobalt salt in-situ growth ZIF-67, so that the cobalt-based modified industrial solid waste vinegar residue derived carbon composite material is obtained. Porous activation is carried out on the surface of the carbonized vinegar residues through hot alkali, rich protruding wrinkles and pores are formed, the surface area is increased, rich nitrogen components and active carbon oxygen functional groups are reserved, and the catalytic reaction activity is improved. The material is used as a sensing material to prepare a high-performance electrochemical biosensor, and the constructed electrochemical sensor is used for detecting biomolecules such as hydrogen peroxide and the like to obtain a relatively wide detection range.

The current disclosure concerns a system for producing biomolecules comprising a docking station (30), said docking station encompasses: —a bioreactor (1); —a concentrator (2) fluidly linked to the bioreactor; —an intermediate vessel (4), positioned between said bioreactor (1) and concentrator (2), wherein said intermediate vessel (4) and concentrator (2) are connected by a retentate conduit (303), allowing recirculating of liquid from an output of the concentrator to an input of said intermediate vessel and—a controller, integrated in said docking station (30), which is able to control the biomolecule process.

The invention provides a nucleic acid aptamer and a screening method thereof, the aptamer is of an oligonucleotide DNA structure, the nucleotide sequence of the nucleic acid aptamer is the nucleotide sequence of any DNA fragment as shown in SEQ ID NO: 1-9, and the nucleic acid aptamer can specifically target cardiac muscle cells (CMs). According to the invention, a new strategy can be provided for clinical treatment of cardiovascular diseases, and meanwhile, bioactive molecules such as microRNA (miRNA), small interfering RNA (small interfering RNA, siRNA), lipidosome, microspheres, microcapsules and the like can be carried to be used as an intracellular drug delivery tool.

Disclosed herein are systems and methods for direct classification of biological datasets. The datasets may include raw mass spectrometry data. Some aspects include training a classifier for direct classification of raw data, and some aspects include applying the classifier.

Disclosed herein are methods of generating therapeutic biomimetic nanovesicles (BioNVs) or therapeutic exosomes with lumen-loaded therapy-related biomolecules from low immunogen cells, compositions of therapeutic BioNVs or therapeutic exosomes, and methods of treating or preventing diseases or conditions using the same.