526 results about "Biomolecule" patented technology

The present disclosure relates to compositions and methods for reversible fixation of biological samples using fixation reagents that form bis-carbamate crosslinks between amine-bearing moieties in biomolecules.

The invention discloses a lipid compound capable of being used for gene delivery, a preparation method of the lipid compound and application of the lipid compound in gene delivery. Also disclosed herein is a lipid nanoparticle comprising the lipid compound, a gene delivery composition comprising the lipid compound or the lipid nanoparticle. The lipid compounds, lipid nanoparticles and delivery systems herein enable efficient complexation, protection, intracellular and targeted delivery and release of biomolecules, such as oligonucleotides and nucleic acids, in vitro and in vivo tissues and organs.

The embodiment of the invention discloses a terahertz virus spectrum classification method and system based on transfer learning, and the method comprises the steps: obtaining a source domain terahertz spectrum data set containing various biomolecule hydrogen bond vibrations, and obtaining an initial feature extraction model; extracting a hydrogen bond vibration characteristic mode in a preset frequency band range, and taking a characteristic center of the hydrogen bond vibration characteristic mode as a cross-domain bridging characteristic; obtaining target sample data, wherein the sample data is a small sample data set; converting the similarity between the feature center and a target sample spectrum peak into a dynamic weight of a migration loss function, and performing layer-by-layer fine adjustment on the initial feature extraction model network to obtain a virus classification model; the fine tuning process comprises the following steps: adjusting a weight factor in real time according to the spectrum peak offset of the current batch of samples; inputting the terahertz spectrum data of the to-be-detected virus into the virus classification model to obtain a classification result of the to-be-detected virus. According to the application, the accuracy, robustness and industrial applicability of terahertz virus detection are remarkably improved.

This invention is related to the field of biosensors. In particular, devices and methods are described which enable the quantification of biomolecules by virtue of particle speed driven by induced-charge electrophoresis. For example, a range of biomolecules can be simultaneously detected in a highly sensitive manner from a library (e.g., combination) of active particles having different shapes or fluorescent signatures. Such biomolecules are related to conditions including, but not limited to, cancer biomarkers, viral infection biomarkers, toxins, pesticides, and small molecule drugs.

The invention provides a nucleic acid aptamer and a screening method thereof, the aptamer is of an oligonucleotide DNA structure, the nucleotide sequence of the nucleic acid aptamer is the nucleotide sequence of any DNA fragment as shown in SEQ ID NO: 1-9, and the nucleic acid aptamer can specifically target cardiac muscle cells (CMs). According to the invention, a new strategy can be provided for clinical treatment of cardiovascular diseases, and meanwhile, bioactive molecules such as microRNA (miRNA), small interfering RNA (small interfering RNA, siRNA), lipidosome, microspheres, microcapsules and the like can be carried to be used as an intracellular drug delivery tool.

The invention provides a defect early warning method and device for multi-source heterogeneous data fusion of power equipment, and the method comprises the steps: collecting biological data, quantum data and operation data of the power equipment; performing biomolecular diagnosis on the biological data to obtain biological characteristic information; laying a quantum cobweb based on the quantum data to obtain quantum feature information; performing feature fusion on the biological feature information and the quantum feature information to obtain feature fusion information; performing space-time coding conversion on the feature fusion information and extracting bionic features to obtain space-time feature information; generating a space-time pulse characteristic flow based on the space-time characteristic information; constructing a holographic decision field through the space-time pulse characteristic flow; performing risk deduction by using the holographic decision field to generate a defect evolution cloud picture; and generating defect early warning information based on the defect evolution cloud atlas and the operation data. According to the method, potential defects of the equipment can be accurately found in advance, the equipment failure rate is reduced, and the reliability and stability of the power system are improved.

The invention discloses a zinc transition metal complex and preparation and application thereof in fluorescence recognition of biomolecules, and relates to the technical field of metal organic framework materials, the chemical formula of the zinc transition metal complex is [Zn2 (L) 2 (bpe) (H2O) 2] n, and 3, 5-bis (3-carboxylic acid phenyl)-1, 2, 4-triazole, 1, 2, 4-triazole, 1, 3, 4-triazole, 1, 3, 4-triazole, 1, 3, 4-triazole, 1, 3, 4-triazole, 1, 3, 4-triazole, 1, 3, 4-triazole, 1, 3, 4-triazole, 1, 3, 4-triazole The complex material is prepared by co-dissolving 2, 2-bis (4-pyridyl) ethylene, Zn (NO3) 3.6 H2O and N, N-dimethylformamide in an aqueous solution, has excellent fluorescence performance, has the capability of specifically identifying ascorbic acid in various biomolecules, and also has relatively good anti-interference performance, excellent circularity and strong stability, and can be widely applied to the field of biochemistry, biochemistry, biochemistry, biochemistry, biochemistry, biochemistry, biochemistry, biochemistry, biochemistry, biochemistry, biochemistry, biochemistry, biochemistry, biochemistry, biochemistry and biochemistry. The method can be used for detecting ascorbic acid in water and beverages.

Described herein are methods for screening for a disease state. The method may include obtaining multiple data sets and identifying the disease state based on a combination of the data sets. The data sets may include biomolecule measurements obtained by multiple methods, such as through the use of particles and reference biomolecules.

The invention belongs to the technical field of biomolecular interaction detection, and particularly relates to a method for synergistically analyzing a molecular interaction state based on multimodal optical parameters of a rare earth up-conversion nano material. The method comprises the following steps: S1, providing a ligand coated rare earth up-conversion nano material UCNPs probe; s2, enabling the rare earth up-conversion nano material UCNPs probe to be in contact with a solution sample to be detected; s3, exciting the rare earth up-conversion nano material UCNPs probe by using near-infrared pulse laser, and collecting the following optical parameters: luminous intensity change, molecular layer thickness / mass change delta D, luminous life change delta tau and emission peak displacement delta lambda; and S4, based on at least two of the parameters, cooperatively analyzing and judging the interaction state of the ligand on the surface of the probe and the molecules in the solution sample to be detected. The molecular interaction state is synergistically analyzed based on changes of multiple optical parameters, and the technical problem that an existing molecular interaction analysis technology cannot be applied to complex biological samples (serum and cell lysis buffer) is solved.

The invention discloses an aza-BODIPY biomedical phototherapy agent taking oxajulolidine as a donor and a preparation method thereof, and relates to a medical phototherapy agent for treating brain glioma and a preparation method thereof, and the prepared compound is prepared by introducing an oxajulolidine fragment with electron donating property into a key position of an aza-BODIPY skeleton, so that the aza-BODIPY biomedical phototherapy agent is obtained. The donor-acceptor type molecular near-infrared phototherapy agent with a new structure is constructed, has good near-infrared absorption and emission performance and excellent stability, and shows a remarkable effect in the field of cancer photodynamic (PDT)-photothermal (PTT) synergistic treatment. The structural categories of the near-infrared aza-BODIPY biomolecules are enriched, and a new molecular platform is provided for expansion and application of the near-infrared aza-BODIPY biomolecules in the fields of biological imaging, tumor diagnosis and treatment, functional materials and the like.

A polyelectrolyte-surfactant complex, a method of removing a toxic substance from contaminated soil or contaminated groundwater using a polyelectrolyte-surfactant complex and a method of making a polyelectrolyte-surfactant complex. The polyelectrolyte-surfactant complex is made from a complexated recombinant intrinsically disordered protein that has been electrostatically conjugated to an anionic surfactant to provide enhanced liquid-liquid phase separation properties.

The present disclosure provides a method for the chemoselective modification of a target molecule. The subject methods comprise contacting a target molecule comprising a thiol moiety with a biomolecule comprising a reactive moiety, wherein the reactive moiety is produced by reacting a biomolecule comprising a phenol moiety or catechol with an enzyme capable of oxidizing the phenol moiety or the catechol moiety. The contacting is performed under conditions sufficient to conjugate the target molecule to the biomolecule, thereby producing a modified target molecule. The present disclosure provides compositions comprising a subject target molecule comprising a thiol moiety and a biomolecule comprising a phenol moiety or a catechol moiety. The present disclosure provides kits for performing the subject methods. The disclosure also provides modified target molecules and methods of using the same.

The invention discloses a method for predicting multi-type association in a miRNA-LncRNA-disease network through a simplified input strategy, and relates to the technical field of biomolecular association prediction, and the method comprises the following steps: data acquisition and preprocessing, heterogeneous network construction, deep feature extraction, feature fusion, multi-task joint prediction and result output. Innovation and breakthrough of correlation prediction of multiple types of biomolecules are achieved by simplifying an input strategy and a depth feature fusion mechanism, a verified binary correlation matrix is adopted to replace a traditional similarity network, redundant calculation noise can be effectively avoided, and the correlation prediction of multiple types of biomolecules is achieved by stacking a depth matrix decomposition module. Low-dimensional features are gradually extracted by adopting an encoder-decoder cascade structure, known associated signals can be enhanced, false negative interference can be inhibited, and by introducing a dynamic weighted feature fusion and multi-task joint prediction framework, the collaborative reasoning capability of three types of associations can be synchronously optimized, and the data collection complexity is remarkably reduced.

The invention relates to the technical field of signal decoding, in particular to a single-molecule signal decoding method based on an artificial intelligence algorithm, which specifically comprises the following steps: performing baseline correction on an original current signal sequence to obtain a corrected current signal dynamic baseline sequence; conducting conductance fluctuation component separation through power spectrum density and frequency band division to obtain a main current signal of the molecular event; dividing into independent molecular event segments, and extracting stable features to obtain a complete local segment stable feature set; splicing and correcting to obtain a corrected complete single molecule track; and performing similarity contrastive analysis with a standard control single-molecule trajectory to obtain an optimized single-molecule trajectory. According to the method, the problem that in the prior art, the application range and precision of the technology in complex biomolecule analysis are limited due to the fact that a single-molecule signal is difficult to be accurately matched with a standard control track in a high-salt environment is solved.

The invention provides a paramagnetic micro-nano magnetic particle magnetophoresis separation device and a paramagnetic micro-nano magnetic particle magnetophoresis separation method, and relates to the technical field of micro-nano magnetic particle separation and magnetic labeled biomolecule separation analysis. The separation device comprises a plurality of permanent magnets which have the same magnetism and the same size and are arranged above the separation channel at intervals in the direction of the separation channel, and a plurality of sets of mechanical structures which respectively drive each permanent magnet to move up and down in the vertical direction; the Hall sensor clings to the separation channel and is used for detecting the magnetic induction intensity at the separation channel; a liquid inlet device; a UV / Vis ultraviolet-visible light detector; a collection device and a computer. The magnetic induction intensity in the separation channel can be set, the critical diameter of the micro-nano magnetic particles capable of being captured by the magnetic field is determined, magnetophoresis separation of paramagnetic micro-nano magnetic particles is accurately achieved, the controllability of the magnetophoresis separation process is enhanced, and the separation effect of the micro-nano magnetic particles is improved.

The invention discloses a radioactive medical isotope Ra-223 stably-labeled rare earth nano diagnosis and treatment preparation as well as a preparation method and application thereof. According to the invention, by constructing a dual protection mechanism of the shell layer and the functional biomolecular layer, the Ra-223 is effectively packaged, the recoil nuclei of the Ra-223 are reserved at the tumor part and are prevented from escaping to the surrounding environment of the tumor, the safety of the Ra-223 treatment process can be enhanced, the radioactive energy is ensured to accurately act on the focus, and the treatment effect of the Ra-223 is improved. Meanwhile, the damage risk to healthy tissues is minimized.

The embodiments of the present disclosure relate to decreasing the bioavailability of one or more target biomolecules by providing a composition that comprises a recombinant plasmid with one or more sequences of micro interfering ribonucleic acid (miRNA). When the recombinant plasmid interacts with a target cell, it causes the target cell to upregulate production of the miRNA, which then decreases the bioavailability of the target biomolecule. In some embodiments of the present disclosure, the target biomolecule is a cytokine or other mediator molecule of an inflammatory response.

The invention relates to a unified system for the extraction and purification of biomolecules using a novel Cloudy-Precipitated (CP) Buffer and an integrated extraction device. The CP Solution remains cloudy even when heated and enables single- or multi-step workflows for isolating nucleic acids without requiring multiple buffers or organic solvents. It contains optimized concentrations of detergents, chaotropic agents, salts, and adsorbents to efficiently lyse cells and minimize processing steps. The associated device incorporates a heating and filtration system that allows separation of target molecules from inhibitors or impurities based on molecular weight. It can operate as a single- or double-step configuration with various filter membrane options. This system reduces time, labor, and environmental impact while enhancing sample quality for molecular diagnostics, research, and therapeutic applications.

An aspect of the disclosure provides a detector for detecting a target biomolecule or composition comprising the target biomolecule, the detector comprising: a housing, configured to couple the detector to a carrier; a detection assembly for containing an active material and a liquid medium, wherein the detection assembly and the housing are mutually configured to provide an indication of a reaction of the active material to the target biomolecule or to the composition; a gas sample inlet provided on the housing and an air mover, wherein the gas sample inlet and the air mover are selectively operable to: draw, into the housing, a sample of air from around the apparatus; and to provide the sample of air to the detection assembly for interaction with the active material; the housing further comprising an indicator output for providing the indication to the carrier.

In one aspect, the disclosure relates to compounds methods for treating or preventing diseases associated with aberrant condensation of a biomolecule in a subject, the method including at least the step of contacting one or more cells in the subject with a fusion protein that includes a J domain protein and a targeting molecule, wherein the targeting molecule binds the biomolecule. In one aspect, the biomolecule can be a target protein that may be mutated and / or include one or more intrinsically disordered regions. In another aspect, the targeting molecule can be a nanobody, but other targeting molecules are also contemplated. In still another aspect, the disclosed method is useful for treating and / or preventing cancers such as blood cancers and non-small cell lung cancer. Also disclosed are methods for disrupting condensates in cell culture.

The present disclosure relates to a novel fluorogenic dimer compound, useful as a probe for detection of endogenous receptors, in particular G protein-coupled receptors. The present invention provides compositions and kits comprising such compound and methods of labeling a biomolecule, comprising the step of contacting the biomolecule with the compound of the invention.

The invention relates to the field of medical pipe fitting materials, in particular to an antibacterial and anti-adhesion plastic material for medical pipe fittings and a preparation method of the plastic material, and aims to solve the problems that an existing medical pipe fitting material is poor in antibacterial performance and anti-adhesion performance and cannot meet the use requirements of the medical pipe fittings. According to the preparation method, by compounding the double-ion powder and polyvinyl chloride, the double-ion powder can be stably dispersed in the plastic material, excellent antibacterial performance can be achieved, growth and reproduction of various bacteria can be effectively inhibited, accordingly, the infection risk in the use process of the medical pipe fitting is reduced, the plastic material is endowed with excellent anti-sticking performance, and the service life of the medical pipe fitting is prolonged. The material can reach a bioinert surface, can prevent adhesion of other biomolecules or toxic substances, avoids pollution, also can resist adhesion of tissues, and can be safely applied to medical pipe fittings; meanwhile, the preparation method is simple in process and easy to realize industrial production, the production cost is reduced, and the preparation method has a wide application prospect.

A device for intermittent monitoring of a biomolecule in a human body, and use thereof. The device includes a device casing, a microneedle array including an array of needles configured to extract interstitial fluid from a user, a microfluidic system defining a fluidic channel including a biomolecule debonding agent, wherein the fluidic channel is in fluidic communication with the microneedle array and configured to facilitate flow of the extracted interstitial fluid therethrough, and an assay system in fluidic communication with the microfluidic system. The assay system includes an affinity binding agent capable of generating an indication of a level of one or more biomolecules present in the extracted interstitial fluid. The microneedle array, the microfluidic system, and assay system are disposed within the device casing.

The application discloses a cell-penetrating peptide-oligonucleotide delivery system prepared based on a biological orthogonal click chemical reaction and a preparation method and application thereof. The cell-penetrating peptide with a cell membrane penetration function and the oligonucleotide with a biological function are connected through the biological orthogonal click chemical reaction, so that the biological activity of the biomolecule can be better maintained, a high fidelity is provided for a wide range of functional groups, no obvious toxic side effects are caused, and the oligonucleotide delivery is efficiently realized, thereby providing a new approach for preventing and treating osteoclast activation diseases.

The invention relates to a method capable of monitoring physiological phosphate, in particular to a colorimetric sensor array based on MIL-101 (Fe) and a preparation method and application of the colorimetric sensor array. Nitro and amino are attached to MIL-101 (Fe), and a sensor array is constructed based on the three kinds of Fe-MOFs. Sensing image information of phosphate before and after a reaction in a colorimetric sensor array is obtained, RGB difference values of all colorimetric points before and after the reaction are obtained to obtain an LDA image, the lowest detection limit can be as low as 0.1 mu M. The array not only has good discrimination capability on ten phosphate compounds such as ATP, ADP, AMP, adnosine, Pi, PPi, UTP, GTP, ITP and CTP, but also has good detection effect on phosphate compounds such as phosphate compounds. And under the condition that interferents (ions, amino acids and the like) are contained, accurate identification can be realized. The colorimetric sensor array can also distinguish different PPs in fetal calf serum. The metal-organic framework sensor array has a good prospect as a novel college sensing platform of phosphate and other disease-related biomolecules.

Provided herein are methods, and compositions for the detection and analysis of biomolecule interactions a microfluidic device. The detection and analysis occurs in aqueous droplets within an oil layer having surfactants.

The invention discloses construction and application of a biosensor based on an efficient antifouling interface and dual signal amplification, and belongs to the technical fields of optical / electric analytical chemistry, biosensing and material science. According to the invention, the electrode interface is modified with polyaniline doped with polytannic acid as an antifouling film, so that non-specific adsorption of interfering biomolecules in a complex medium is resisted; euTb-MOF is prepared as an efficient luminous body, Tb < 3 + > can transfer energy of Tb < 3 + > and ligand to Eu < 3 + >, and effective signal self-enhancement is realized; according to the EuTb-MOF, the reduction of S2O8 < 2-> is promoted by utilizing the reversible conversion of valence states of Co < 2 + > / Co < 3 + > and Fe < 2 + > / Fe < 3 + > and the synergistic catalysis of Ag NPs, so that the electrochemical luminescence signal of the EuTb-MOF is further improved. Based on the above strategy, the constructed biosensor has high detection sensitivity and precision and long service life, the linear range is 10 fM to 100 nM, and the detection limit is as low as 3.62 fM.

Disclosed herein are formulations, substrates, and arrays. Also disclosed herein are methods for manufacturing and using the formulations, substrates, and arrays. Also disclosed are methods for identifying peptide sequences useful for diagnosis and treatment of disorders, and methods for using the peptide sequences for diagnosis and treatment of disorders, e.g., celiac disorder. In certain embodiments, substrates and arrays comprise a porous layer for synthesis and attachment of polymers or biomolecules.

Provided herein are methods of detecting unlabeled biomolecules. In some embodiments, the methods include disrupting a cell population sufficient to release unlabeled biomolecules from the cell population to produce released biomolecules in which the cell population is disposed on a first inner surface of a chamber that is disposed substantially within a fluidic device and in which the chamber comprises a fluidic material. In some embodiments, the methods also include binding the released biomolecules to a second inner surface of the chamber to produce surface-bound biomolecules, introducing an incident light toward the second inner surface of the chamber concurrent with, and / or after, producing the surface-bound biomolecules, and detecting light scattered by the surface-bound biomolecules over a duration to produce a set of biomolecule imaging data. Related fluidic devices, systems, and computer readable media are also provided.

Orally administrable nanoparticle complexes of biological molecules and biopolymers are described herein, and include formulations for the oral administration of peptides, proteins, nucleic acids, and antibodies.