The application belongs to the technical field of
biotechnology, and particularly relates to a
lysosome-targeting probe and a preparation method and application thereof. The
structural formula of the
lysosome-targeting probe is shown in formula (I). In formula (I), A is selected from an
oxygen atom and a
sulfur atom, R1 is selected from a
hydrogen atom and an aromatic ring group, R2 is selected from a cyano group and a group shown in formula (II), R3 is selected from an aromatic ring group, and R4 is selected from a
hydrogen atom and an
alkyl group with a
carbon atom number of 1-20. The compound of the application can specifically target lysosomes in
tumor cells, emit bright
fluorescence, and has a good
fluorescence co-localization effect with lysosomes in
tumor cells. The compound can be used for
lysosome imaging, and the
fluorescence intensity thereof is positively correlated with the
viscosity of lysosomes in living cells such as
tumor cells. The compound can be used for monitoring the
viscosity change of lysosomes in living cells under various conditions. The compound can be used as a
photosensitizer and a treatment sensitizer, specifically ablates tumors through
photodynamic therapy (PDT), restores the sensitivity of tumor cells to radiotherapy and
chemotherapy, and enhances the
treatment effect of radiotherapy and
chemotherapy.