The utility model provides a
perfusion device and biological sample
microstructure observation system, including device main part, device main part is provided with liquid
inlet channel, liquid outlet channel and sample storehouse, sample storehouse only bottom is open, liquid
inlet channel communicates device main part outside with sample storehouse, liquid outlet channel communicates device main part outside with sample storehouse. The position, corresponding to the sample bin, of the bottom of the device body is used for being connected with the
cover glass, the top edge of the
cover glass is detachably connected with the bottom face of the device body, and after connection, the
cover glass completely seals the sample bin. According to the
perfusion device, the liquid
medicine of the preset type or the preset concentration can be conveyed into the sample bin through the liquid
inlet channel, then the effect of rapidly replacing the type of the liquid
medicine or accurately regulating and controlling the concentration of the liquid
medicine is achieved, when the biological samples need to be replaced, only the cover glass loaded with different biological samples needs to be replaced, and operation is convenient. The in-situ observation effect of the biological sample can be realized.