129 results about "Natural bone" patented technology

The invention belongs to the field of biomedical materials, and particularly discloses a preparation method and application of a 3D printing porous composite scaffold. I-type collagen and silk fibroin composite gel is modified on a 3D printing three-element doped hydroxyapatite / polycaprolactone (PCL) composite scaffold with a polydopamine coating, the three-element doped hydroxyapatite is synthesized by using a chemical precipitation method, and Mg < 2 + >, Sr < 2 + > and SeO3 < 2-> ions are doped in crystal lattices. The 3D printing porous composite scaffold provided by the invention effectively simulates the structure and function of soft and hard combination of natural bones, is helpful for regulating and controlling the stem cell fate to improve the bone regeneration process, repairs bone defects while eliminating tumors, and can be applied to bone defect repair or osteosarcoma treatment.

The invention relates to bionic matrix hydrogel for constructing bone organs and a preparation method of the bionic matrix hydrogel. The bionic matrix hydrogel is prepared from a bone acellular extracellular matrix, a salmon DNA (Deoxyribose Nucleic Acid) network and a calcium phosphate oligomer under the combination of photo-crosslinking and self-assembly physical crosslinking. The acellular extracellular matrix in the bionic matrix hydrogel can simulate organic components in natural bones, the calcium phosphate oligomer can simulate inorganic components in the natural bones, and sufficient phosphate ions can be provided for biomineralization due to introduction of a DNA network. The biomimetic matrix hydrogel can replace Matrigel to act in bone organoid culture, provide a living environment similar to bone tissue for three-dimensional culture cells, enhance substance exchange and signal transduction between cells, promote proliferation, adhesion and osteogenic differentiation of bone marrow mesenchymal stem cells, and further accelerate the process of biomineralization. The invention provides an optional new material for preparing the functional bone organ.

The invention discloses a bionic lantern frame composite internal fixing device and a preparation method, and relates to the technical field of orthopedic medical instruments, the bionic lantern frame composite internal fixing device comprises an implant and a multi-rod bridging structure, the multi-rod bridging structure is composed of a plurality of longitudinal connecting rods and transverse locking nails, and the implant is composed of a plurality of multi-level bionic structures; the multi-layer bionic structure comprises two hexagonal upper and lower layer structures which are symmetrically arranged and a middle spiral layer structure arranged between the two hexagonal upper and lower layer structures. According to the invention, inspired from honeycomb and ox horn macro-microstructure, the multi-level bionic structure prepared by adopting a coupling bionic technology has excellent mechanical properties and strong buffering and vibration absorbing capabilities, can be effectively matched with the mechanical properties of natural bones, and can effectively avoid secondary injury and stress shielding effect after implantation of a patient; the composite coating introduced through an electrochemical deposition method solves the problems that a single implant structure is insufficient in biocompatibility, insufficient in antibacterial property and weak in osteogenic differentiation capacity.

The invention relates to a method for constructing an injectable bone organ to realize rapid bone regeneration. The method comprises the following steps: 1, obtaining a water-soluble periosteum acellular matrix by adopting an acellular technology; secondly, compound bone salt is prepared according to the proportion of natural bone salt components, the water-soluble periosteum acellular matrix and the compound bone salt are mixed according to the proportion of natural bone organic and inorganic components to form a bionic matrix component, and then the bionic matrix component is added into gelatin to construct a completely bionic osteogenesis microenvironment; and 3, constructing a bone regeneration material to form a bone organ, firstly preparing a granular gelatin scaffold with a full-bionic osteogenesis microenvironment, then inoculating the bone marrow mesenchymal stem cells, finally performing in-vitro culture, and regulating the differentiation and secretion of the bone marrow mesenchymal stem cells by virtue of the osteogenesis microenvironment of the scaffold to finally form the bone organ.

The invention belongs to the technical field of biomedical materials, and particularly relates to a gradient bionic wood-based collagen bone-cartilage repair scaffold and a preparation method and application thereof.The scaffold comprises a surface layer, a middle layer and a bottom layer, the surface layer is composed of collagen, the middle layer is composed of collagen and nano-hydroxyapatite, and the bottom layer is composed of collagen and nano-hydroxyapatite; the bottom layer is composed of wood, collagen and nano-hydroxyapatite, the scaffold is obtained by stacking the surface layer, the middle layer and the bottom layer and then conducting freeze-drying, the structure of the scaffold is similar to that of natural osteochondral cartilage, the surface layer is of a porous structure communicated with one another, and the bottom layer is of a directionally-arranged pore channel structure; the main composition of a natural bone cartilage tissue can be highly simulated, and the gradient is presented; the bone marrow mesenchymal stem cell prepared by the method has the advantages of high mechanical performance, gradient enhancement from the surface layer to the bottom layer, excellent biocompatibility and biological activity, capability of remarkably promoting cell proliferation and gradient differentiation of bone marrow mesenchymal stem cells, and application to osteochondral tissue regeneration and clinical treatment of osteoarthritis.

The invention relates to a collagen scaffold with a fixed-axis orientation structure and a preparation method and application thereof, and the preparation method comprises the following steps: (1) dropwise adding a soluble phosphate solution into a collagen-soluble calcium salt mixed solution, and adjusting the pH value with ammonia water; (2) injecting the obtained mixed solution into a mold of which the bottom is provided with a heat-conducting bottom plate; vacuumizing is conducted, heat insulation treatment is conducted on the part, except for the heat conduction bottom plate, of the mold, and then the mold is frozen and formed at the temperature ranging from-20 DEG C to-180 DEG C; and (3) freeze drying. According to the preparation method, heat insulation treatment is conducted on the part, except for a heat conduction bottom plate, of a mold, only the heat conduction bottom plate is left for heat exchange with the external environment, during freezing, low temperature is gradually transmitted upwards from the heat conduction bottom plate to form a temperature gradient, ice crystals of an oriented structure are formed, and then the ice crystals are removed through freeze drying. The interior of the scaffold can be provided with a structure which is similar to natural bones and is arranged in a Harvard system, so that osteoblast proliferation is facilitated, and good conditions are created for growth of cells and blood vessels.

The present invention belongs to the field of biomedical engineering technology and relates to a bone organoid, its construction method, and its use. The bone organoid is obtained by three-dimensionally culturing a cell mixture comprising stem cells and macrophages. The bone organoid and its construction method of the present invention enable rapid preparation of bone organoids with minimal differences from natural bone, enabling regulation of cell fate and better bone formation and repair.

Porous-based bone implants with the integration of Triply Periodic Minimal Surface, TPMS, porous architectures are designed to support the growth and proliferation of bone tissue, bone marrow, and capillaries. This disclosure intends to reduce the adverse effect of conventional implants such as bone resorption over time, which is called the “stress shielding effect”. The “stress shielding effect” is caused by the mismatch between the implant and natural bone stiffness. Triply Periodic Minimal Surface, TPMS, porous architectures exhibit interconnected pore features. The interconnection of the porous network allows the TPMS to have a higher permeability than that of other porous structures, leading to more favorable nutrient transport. In addition, many physical characteristics of the TPMS structures including surface-to-volume ratio, pore size, elastic modulus, and fluid behaviors can be controlled precisely through mathematical manipulation. As a result, TPMS-based implants could be physical features, which are vaned based on different bone regions. In other words, the medical implants may exhibit non-uniform or gradient physical features, which can match the characteristic of trabecular and cortical bones. Therefore, TPMS-based implants could adjust the features to mimic neighboring bone regions. As a result, we can achieve medical implants, which have superior mechanical and biological responses, resulting in optimal cell growth and better medical treatment.

The invention provides a preparation method of a gradient bionic rotator cuff repair stent, the stent prepared by the method and application. The method comprises the following steps that 1, a gradient fiber structure is designed through conventional 3D modeling software; step 2, adding the raw materials into the barrel material, and printing a tendon scaffold by adopting a near-field direct-writing melting technology; 3, raw materials in the barrel material are replaced, and the cartilage scaffold is printed through the near-field direct-writing melting technology; optionally, replacing the raw materials in the barrel material, and printing the mineralized cartilage scaffold by adopting a near-field direct-writing melting technology; and 4, replacing the raw materials in the barrel material, and printing the bone scaffold by adopting a near-field direct-writing fusion technology. The stent disclosed by the invention has a structural gradient and a component gradient, is matched with natural bone tissues, and can better promote the repair of the bone tissues.

The invention discloses a method for designing a bionic bone scaffold based on wall thickness mixed gradient change, and belongs to the technical field of bionic bone scaffolds. By designing the wall thickness mixed gradient function, the lattice unit can have wall thickness gradient change in the radial direction and the axial direction at the same time, so that the porosity also has gradient change in three directions at the same time, and the defect that a bone scaffold is designed by using a lattice material (beam unit) unit and a TPMS unit in the prior art can be well overcome; and the stent better conforms to the natural bone structure and mechanical property requirements. The designed bionic bone scaffold is excellent in mechanical property, high in compression resistance and deformation resistance and capable of promoting cell growth and tissue regeneration.

The invention discloses a design method of a bionic bone scaffold based on a TPMS (Tire Pressure Monitor System), and belongs to the technical field of bionic bone scaffolds. According to the method, on the basis of a G unit formula and a D unit formula, a bias function is adjusted to generate a G gradient unit CAD model and a D homogeneous unit CAD model; fusing the CAD model of the G gradient unit and the CAD model of the D homogeneous unit by using a fusion function to generate a GD radial fusion CAD model, and adjusting an offset function of the G gradient unit by adopting an interpolation method to enable the GD radial fusion CAD model to reach a target average porosity; reducing the wall thickness of the GD radial fusion CAD model to form a GD radial fusion CAD model with the wall thickness; then, a finite element simulation grid model is divided, and an inp-format simulation model file is output; importing the simulation model file in the inp format into HyperMesh software for repairing, and dividing grids to export the model in the inp file format. According to the porous scaffold designed by the invention, different functional grades of a natural bone structure are considered, and different unit cell polymorphic fusion is carried out, so that the porous scaffold better conforms to the mechanical properties of natural thighbones, and the specific surface area is increased by virtue of the lightweight design, so that more space is provided for bone cell adhesion and growth.

The invention relates to the technical field of biomedical material manufacturing, and particularly discloses a gradient composite scaffold 3D printing process based on AI topological optimization, which comprises the following steps: a) based on medical image data of a patient, generating a digital model with porosity gradient distribution of 20-200 [mu] m and PCL / MCC material proportion gradient change through AI topological optimization; b) a double-nozzle FDM system is adopted to implement gradient printing, the layer thickness is 50 microns, the material switching frequency is 20 Hz, and the gradient resolution is 5% / layer; c), an electrostatic spinning nanofiber layer is deposited after FDM printing of each layer, the fiber diameter is 500 + / -50 nm, and the deposition density is 10-20 g / m. The method has the beneficial effects that through finite element analysis verification, the AI topological optimization enables the matching degree of the porosity and the component gradient of the stent and the natural bone to be greater than 90%, the mechanical distribution is more uniform, and the stress concentration is avoided; by adopting a double-nozzle FDM (20Hz switching frequency) and electrostatic spinning composite process, the interlayer shear strength is improved to 5.8 MPa, and the problem of traditional interface layering is solved.

The invention discloses a preparation method of a bionic beetle skeleton implant, and belongs to the field of femoral implantation. The bionic structure characteristics of beetle elytra and cuticle are creatively combined, the mechanical strength and structural stability of the porous nickel-titanium alloy structure implant are effectively improved through the bionic structure design method, and the long-standing stress shielding effect problem of a traditional femoral metal implant is successfully solved; the porous nickel-titanium alloy bionic skeleton structure prepared by adopting a selective laser melting (SLM) technology not only has good mechanical suitability, avoids rejection and shedding reaction with natural bone tissues after implantation, but also can realize a complete shape memory recovery function in a room temperature environment, and has good application prospects in the field of medical orthopedic implantation.

The invention provides a composite material porous bone repair scaffold as well as a preparation method and application thereof. The composite material porous bone repair scaffold is prepared from the following raw materials: a bone repair organic material, a bone repair inorganic material and poly (citric acid-1, 8-octylene glycol / ethylene glycol) ester particles. The biodegradable poly (citric acid-1, 8-octylene glycol / ethylene glycol) ester particles are added into the bone repair organic material and the bone repair inorganic material, so that the degradation rate of the bone repair scaffold can be regulated and controlled, and the degradation rate of the composite material is matched with the new bone regeneration rate; moreover, the bone repair scaffold has good biocompatibility and high osteogenic activity, the mechanical property of the bone repair scaffold can be improved, the mechanical behavior of natural bones can be simulated, and the stability of the scaffold can be improved.

The invention belongs to the field of biomedical materials, and particularly discloses a preparation method and application of a 3D printing porous composite scaffold. I-type collagen containing growth factors and silk fibroin composite gel are modified on a 3D printing three-element doped hydroxyapatite / polycaprolactone (PCL) composite scaffold with a polydopamine coating, the three-element doped hydroxyapatite is synthesized by using a chemical precipitation method, and Zn < 2 + >, Sr < 2 + > and SeO < 3-> ions are doped in crystal lattices. The 3D printing porous composite scaffold provided by the invention effectively simulates the structure and function of soft and hard combination of natural bones, is helpful for regulating and controlling the stem cell fate to improve the bone regeneration process, repairs bone defects while eliminating tumors, and can be applied to bone defect repair or osteosarcoma treatment.

The invention discloses a method for designing a bionic femoral stent based on fusion of a VT structure and a TPMS structure, and belongs to the technical field of bionic bone stents. The method comprises the following steps: respectively creating a Diamond unit homogeneous porous structure and a VT porous structure; and creating a Ramp fusion function, substituting the homogeneous porous structure of the Diamond unit and the Ramp fusion function into a Mix instruction, and then sequentially substituting the VT porous structures into the Mix instruction to generate a fusion model. The homogeneous porous structure of the Diamond unit and the VT porous structure are subjected to radial smooth fusion, smooth connection is realized at the fusion position, the service life of the porous structure serving as the bionic bone scaffold is shortened, the smooth surface of the D unit and the periodic structure uniformly conduct stress, the rounded Thiessen polygonal structure further disperses stress, and the stress dispersion and synergistic effects are achieved. The bionic femoral stent constructed by the invention can simulate natural bones, promote cell adhesion, proliferation and differentiation, and optimize transportation of nutrient substances and metabolic wastes. And the smooth fusion interface reduces cell damage and reduces immunological rejection.

The invention relates to the technical field of medical implants, in particular to a 4D printing-based porous Niti alloy bionic bone gradient structure construction method, which comprises the steps of patient bone data acquisition, bionic gradient model design, NiTi powder preparation, 4D printing, thermal treatment activation of memory effect, surface functionalization treatment and mechanical / biological performance test. The method is characterized by comprising the following preparation steps: accurately acquiring bone structure data through CT / MRI, combining Mimics pretreatment and UG / NX modeling, designing aperture and porosity for differentiation of cortical bone (low porosity of 30%-40%) and cancellous bone (high porosity of 50%-70%), simulating typical load working conditions through ANSYS, enabling the elastic modulus (3-15 GPa) and compressive strength (80-200 MPa) to be close to natural bone, and finally obtaining the bone structure data through the CT / MRI, the pore diameter and porosity of the cortical bone (low porosity of 30%-40%) and the porosity of the cancellous bone (high porosity of 50%-70%). According to the design, the elastic modulus difference between the implant and the host bone is remarkably reduced, the bone resorption problem caused by stress shielding is avoided, meanwhile, continuous porosity transition is achieved through the power gradual change technology, and the structural stability under the complex load is improved.

The invention relates to the technical field of biomedical materials, and discloses a 3D printing stent for bone marrow mesenchymal stem cell culture and application of the 3D printing stent, a zinc-magnesium alloy is used as a base material for laser 3D printing, and then fluorinated coating and fiber coating treatment are performed, so that the biocompatibility and the biological activity of the 3D printing stent are remarkably improved, and the 3D printing stent is suitable for being used for bone marrow mesenchymal stem cell culture. Adhesion and growth of bone marrow mesenchymal stem cells are promoted; the magnesium metal and the zinc metal have mechanical properties similar to those of natural bones, can release magnesium ions and promote osteogenic reaction after being implanted into a body, can be completely degraded into non-toxic substances to be discharged out of the body, and are proved to be potential bone substitute materials; the corrosion resistance of the stent is enhanced by the fluorinated coating, the service life is prolonged, and certain antibacterial and anti-inflammatory characteristics are endowed; sodium alginate and collagen components in the fiber coating layer are beneficial to simulation of an extracellular matrix environment, and cell differentiation and osteogenic reaction are further promoted.

The invention provides an application of a composition in preparation of a bone repair material, the composition is prepared from the following materials in percentage by weight: 70%-90% of coral hydroxyapatite particles, 5%-30% of recombinant human collagen and 0-7% of an excipient, the bone repair material is a cancellous bone imitating material, and the porosity is 85%-99%. The composition provided by the invention has high porosity and a warp-wise gradient structure, can simulate the characteristics of natural bone tissues, has excellent biocompatibility, biodegradability, hydrophilicity, osteogenesis performance and shape memory function, and has excellent clinical use convenience, hemostasis and healing promotion effects and excellent osteogenesis effect; the bone repair material has a wide application prospect in the aspect of preparing the bone repair material for filling and / or repairing bone defects.

The invention provides a natural bone repair material and a preparation method thereof. The material is animal cancellous bone inorganic salt particles or animal cancellous bone inorganic salt bone slices; the invention also relates to a preparation method of the natural bone repair material. The preparation method sequentially comprises the following steps: selecting materials, crushing, cleaning, removing organic matters, efficiently cleaning and drying, calcining and sterilizing. According to the method, inorganic base heating extraction and low-temperature calcination are adopted, so that the method is non-toxic, harmless, safe, efficient, low in organic matter residue and small in damage to the structure of a natural bone material, and the structure and morphology of the natural bone are better reserved; the defects of long construction period, low efficiency, high cost and the like existing in high-temperature calcination and organic reagent removal are avoided. The size of the bone repair material can be controlled, and the obtained bone repair material has certain mechanical strength, is not easy to break after being filled, and can play a good supporting role; and the material also has the advantages of high specific surface area, high porosity, low crystallinity, good hydrophilic performance, good biocompatibility and the like, and is suitable for bone defect repair.

The invention relates to the technical field of biomedical material synthesis, in particular to a synthetic method of a bionic gradient mineralized collagen-polycaprolactone composite material, which comprises the following steps: a) activating the surface of PCL (polycaprolactone) through argon plasma to generate carboxyl groups with the density of more than or equal to 5 * 10 / cm; b) carrying out amination modification on nano-hydroxyapatite (nHA) by adopting APTES, wherein the surface potential of the modified nHA is greater than or equal to + 15 mV; c) covalently grafting aminated nHA to the surface of PCL through an amido bond under the conditions that the molar ratio of EDC / NHS is 1: 2 and the pH is 5.5 + / -0.2, and the grafting density is greater than or equal to 3 * 10 particles / mu m; the method has the beneficial effects that the gradient mineralization structure is subjected to bionic optimization: the concentration and the flow velocity (the inner / outer layer flow velocity ratio is 1: 3) of mineralization liquid are accurately controlled through a microfluidic gradient generator, linear gradual change (the gradient slope is 1.5 + / -0.3 wt% / 100 [mu] m, R is equal to 0.97) of the mineralization degree from 20-30 wt% of the inner layer to 50-60 wt% of the outer layer is realized, the mechanical gradient characteristics of the natural bone are simulated, the compression modulus is increased from 0.8 GPa of the inner layer to 3.2 GPa of the outer layer in a gradient manner, and the mechanical property of the natural bone is improved. The mechanical matching property of the material and the host bone is obviously improved.

The invention discloses a method for designing a bionic bone scaffold based on unit cell size gradient change, and belongs to the field of biological material design. The method comprises the following steps: calculating the overall wall thickness of FCC, TH and G cell size gradient models according to the required porosity; a unit is selected as a unit cell size radial gradient, in order to better simulate a natural bone structure, the overall porous structure morphology is designed as a cylinder model, and the unit cell sizes of the outer layer and the inner layer of each model are designed as required; the overall porosity of the three novel porous structure scaffolds reaches 55% by changing the wall thickness; carrying out mechanical property testing on the novel porous structure; observing the compression deformation behavior of each model and recording the numerical results of the elastic modulus and the yield strength after simulation; the designed novel porous scaffold has different functional gradients in the radial direction, and the application range of the energy absorption material unit in bionic bone scaffold design is expanded.

The present invention relates to a type A recombinant humanized collagen bone repair material and a preparation method and use thereof. The preparation method of the bone repair material provided by the present invention comprises a first step of precipitation method and a second step of dialysis method. By combining the two-step method of precipitation method and dialysis method, the composition and formation process of natural bone are simulated, and a bone repair material with a high collagen ratio and activity is successfully prepared, and collagen can be gradiently degraded and released, collagen expression activity is increased, and the osteogenesis effect is improved.

The invention provides a bionic bone material as well as a preparation method and application thereof. The bionic bone material comprises a photocuring polymer monomer, protein molecules, modified polysaccharide molecules and piezoelectric nanoparticles, the protein molecules and the modified polysaccharide molecules can be crosslinked. Therefore, the bionic bone material disclosed by the invention combines a rigid-soft dual-network structure and the piezoelectric nanoparticles, can simulate mechanical properties, a porous structure and electrophysiological activity of natural bones, and has the characteristics of rapid curing, low thermal damage, high biocompatibility and the like.

A biomimetic intervertebral fusion device with a piezoelectric multifunctional coating belongs to the field of implantable medical devices. It includes an upper piezoelectric multifunctional coating, a fusion device body, and a lower piezoelectric multifunctional coating. A bone graft window is located in the middle of the biomimetic intervertebral fusion device. The piezoelectric multifunctional coating is made of electrospun piezoelectric PVDF / HA fibers, which are conformally adhered to the upper and lower surfaces of the fusion device body through a polydopamine adhesive layer. The upper and lower surfaces of the fusion device body have a biomimetic curved surface configuration to match the morphology of the vertebral endplates, increasing the contact area and reducing stress concentration at the fusion interface. While maintaining an elastic modulus similar to natural bone and good radiation permeability, this fusion device, after implantation, can create a microenvironment conducive to cell adhesion and proliferation. Under the influence of cell traction, ultrasonic stimulation, and the physiological load of the human lumbar spine, it generates electrical stimulation signals, promoting bone fusion, improving the stability of the fusion interface, and reducing the risk of postoperative loosening and subsidence of the fusion device.

The invention discloses a bone repair material as well as a preparation method and application thereof. The bone repair material is prepared from recombinant human collagen, recombinant human bone morphogenetic protein 2 and hydroxyapatite, wherein the ratio of the inorganic matter to the organic matter in the bone repair material is (64-66): (36-34). The bone repair material disclosed by the invention takes the recombinant human collagen as a core raw material, so that the components and the structure of the prepared material are ensured to be highly consistent with those of natural bone tissues; secondly, by adding the recombinant human bone morphogenetic protein 2, the osteogenic activity of the material is further enhanced. The finally obtained recombinant human collagen active bone repair material completely retains the triple helix structure of the recombinant human collagen, so that the recombinant human collagen active bone repair material has complete biological activity, and also has the outstanding advantages of good osteogenic activity, no disease and virus transmission risk and no immunogenicity.

The application discloses a kind of biomimetic silk fibroin cartilage scaffolds for cranium defect repair, belong to biomedical engineering technical field.To solve the problem of single structure, low bone formation efficiency and mismatching of mechanical properties and degradation rate of existing cranium repair scaffold, the application provides a kind of scaffold, which includes in order from outside to inside: biomimetic periosteum layer, bone conduction and vascularization layer and cartilage induction layer.Biomimetic periosteum layer is dense nanofiber membrane structure, and plays the role of physical barrier and osteoinduction;Bone conduction and vascularization layer is gradient porous structure with gradually decreasing aperture from outside to inside, aiming to guide the ordered growth of blood vessels and cells, and promote rapid vascularization;Cartilage induction layer is sponge-like microporous structure, used for forming stable cartilage template and starting endochondral ossification.The application simulates the natural bone healing process through structural biomimicry and functional zoning, synergistically promotes vascularization and osteogenesis, and realizes efficient biological repair of cranium defect.

The application provides an artificial bone material and a preparation method thereof, wherein the material quality of the artificial bone material includes, with the total dry matter of the artificial bone material being 100%, 70%-90% of coral hydroxyapatite particles, 5%-30% of recombinant human collagen, and 0-7% of excipients. The artificial bone material provided by the application has high porosity and a longitudinal gradient structure, can simulate the characteristics of natural bone tissue, and has excellent biocompatibility, biodegradability, hydrophilicity, osteogenic performance and shape memory function.

The invention discloses a medical-grade PEEK composite material for 3D printing and a preparation method of the medical-grade PEEK composite material, relates to the technical field of 3D printing, and solves the problem of low biological activity of an existing PEEK material. The medical-grade PEEK composite material is prepared from the following raw materials in parts by weight: 80-95 parts of a polyether-ether-ketone matrix, 5-15 parts of a bioactive filler, 0.5-5 parts of an interface modifier and 0.1-3 parts of a heat conduction and nucleation enhancer, the bioactive filler comprises nano-hydroxyapatite and bioactive glass in a weight ratio of (1-5): (5-1). The natural bone environment can be effectively simulated, the biological activity of the composite material is improved, and cell adhesion and proliferation are promoted, so that the cell proliferation rate is increased, and the antibacterial effect and the mechanical property of the composite material can be effectively improved.