49 results about "Natural bone" patented technology

The invention provides a natural bone repair material and a preparation method thereof. The material is animal cancellous bone inorganic salt particles or animal cancellous bone inorganic salt bone slices; the invention also relates to a preparation method of the natural bone repair material. The preparation method sequentially comprises the following steps: selecting materials, crushing, cleaning, removing organic matters, efficiently cleaning and drying, calcining and sterilizing. According to the method, inorganic base heating extraction and low-temperature calcination are adopted, so that the method is non-toxic, harmless, safe, efficient, low in organic matter residue and small in damage to the structure of a natural bone material, and the structure and morphology of the natural bone are better reserved; the defects of long construction period, low efficiency, high cost and the like existing in high-temperature calcination and organic reagent removal are avoided. The size of the bone repair material can be controlled, and the obtained bone repair material has certain mechanical strength, is not easy to break after being filled, and can play a good supporting role; and the material also has the advantages of high specific surface area, high porosity, low crystallinity, good hydrophilic performance, good biocompatibility and the like, and is suitable for bone defect repair.

A biomimetic intervertebral fusion device with a piezoelectric multifunctional coating belongs to the field of implantable medical devices. It includes an upper piezoelectric multifunctional coating, a fusion device body, and a lower piezoelectric multifunctional coating. A bone graft window is located in the middle of the biomimetic intervertebral fusion device. The piezoelectric multifunctional coating is made of electrospun piezoelectric PVDF / HA fibers, which are conformally adhered to the upper and lower surfaces of the fusion device body through a polydopamine adhesive layer. The upper and lower surfaces of the fusion device body have a biomimetic curved surface configuration to match the morphology of the vertebral endplates, increasing the contact area and reducing stress concentration at the fusion interface. While maintaining an elastic modulus similar to natural bone and good radiation permeability, this fusion device, after implantation, can create a microenvironment conducive to cell adhesion and proliferation. Under the influence of cell traction, ultrasonic stimulation, and the physiological load of the human lumbar spine, it generates electrical stimulation signals, promoting bone fusion, improving the stability of the fusion interface, and reducing the risk of postoperative loosening and subsidence of the fusion device.

The application discloses a kind of biomimetic silk fibroin cartilage scaffolds for cranium defect repair, belong to biomedical engineering technical field.To solve the problem of single structure, low bone formation efficiency and mismatching of mechanical properties and degradation rate of existing cranium repair scaffold, the application provides a kind of scaffold, which includes in order from outside to inside: biomimetic periosteum layer, bone conduction and vascularization layer and cartilage induction layer.Biomimetic periosteum layer is dense nanofiber membrane structure, and plays the role of physical barrier and osteoinduction;Bone conduction and vascularization layer is gradient porous structure with gradually decreasing aperture from outside to inside, aiming to guide the ordered growth of blood vessels and cells, and promote rapid vascularization;Cartilage induction layer is sponge-like microporous structure, used for forming stable cartilage template and starting endochondral ossification.The application simulates the natural bone healing process through structural biomimicry and functional zoning, synergistically promotes vascularization and osteogenesis, and realizes efficient biological repair of cranium defect.

The invention discloses a medical-grade PEEK composite material for 3D printing and a preparation method of the medical-grade PEEK composite material, relates to the technical field of 3D printing, and solves the problem of low biological activity of an existing PEEK material. The medical-grade PEEK composite material is prepared from the following raw materials in parts by weight: 80-95 parts of a polyether-ether-ketone matrix, 5-15 parts of a bioactive filler, 0.5-5 parts of an interface modifier and 0.1-3 parts of a heat conduction and nucleation enhancer, the bioactive filler comprises nano-hydroxyapatite and bioactive glass in a weight ratio of (1-5): (5-1). The natural bone environment can be effectively simulated, the biological activity of the composite material is improved, and cell adhesion and proliferation are promoted, so that the cell proliferation rate is increased, and the antibacterial effect and the mechanical property of the composite material can be effectively improved.

This invention discloses a cell preparation targeting bone metastatic tumor cells, its preparation method, and its application, belonging to the field of biomedical technology. The cell preparation comprises senescent neutrophils and drug-loaded cationic liposomes internalized within them. The cationic liposomes encapsulate a STING agonist and a pan-photokinase inhibitor. The preparation method includes: preparing drug-loaded liposomes using a thin-film dispersion-ultrasound method; obtaining senescent neutrophils through in vitro culture of extracted neutrophils; and preparing a senescent neutrophil preparation loaded with liposomes. This invention utilizes the natural bone marrow homing ability of senescent neutrophils to achieve precise targeting of bone metastatic tumor cells, and co-delivers two drugs through liposomes, simultaneously activating anti-tumor immunity and inducing tumor cell apoptosis / autophagy, synergistically treating bone metastases. This system has high drug loading capacity, good encapsulation efficiency, and sustained-release effect, and the preparation process is simple and controllable, providing a new strategy for the treatment of cancer bone metastases.

Described herein are three-dimensional scaffold compositions and methods for bone repair or regeneration. In some embodiments, the disclosed scaffolds comprise a core portion mimicking a native trabecular bone structure that is surrounded by an exterior portion mimicking a native cortical bone structure. In some embodiments, the scaffolds may be functionalized by mineralization and / or pre-vascularization to promote bone and blood vessel formation.

The application belongs to the technical field of bone tissue engineering, and particularly relates to a recombinant collagen-chitosan-PEG-hydroxyapatite bone repair scaffold material, a preparation method and application. The bone repair scaffold material is formed into a one-way ordered three-dimensional scaffold by a directional freezing method through recombinant collagen, chitosan and PEG. Nano-hydroxyapatite is uniformly deposited in the inside and surface through a special mineralization process. The prepared material has a very good bionic natural bone structure and composition, a good reticular structure and mechanical strength, good biocompatibility and cell activity, a significant repair effect on critical size bone defects, a good osteogenic induction effect and bone conductivity. The bone repair scaffold material provides a new potential treatment method for severe bone defects, and has a wide application in the fields of artificial bone, artificial cartilage, biological scaffolds and the like.

This invention discloses a biomimetic corncob scaffold with piezoelectric, antibacterial, osteogenic, and healing-promoting functions, comprising: extraction of corncob cellulose nanofibers (CNF); preparation of 3D printing ink; 3D printing of a porous scaffold; loading of antimicrobial peptides and BMP-2 onto a GelMA+PAAM composite hydrogel; and composite preparation of the scaffold and hydrogel. Under external stimulation, the scaffold generates a microcurrent / surface charge, directly disrupting bacterial cell membranes; it avoids drug release and resistance issues, employing a physical mechanism of action with a sustainable response. The microcurrent / electric field generated by the piezoelectric effect simulates the bioelectric environment of natural bone, directly stimulating osteoblast proliferation and differentiation, and promoting mineralization; it mimics the natural anisotropic porous structure of the corncob, facilitating nutrient transport and cell migration; the piezoelectric material itself provides both antibacterial and osteogenic functions, achieving synergistic and unified mechanisms; it is suitable for harsh environments, biodegradable, and has flexible activation methods.

The present invention relates to a customized device made of inert and rigid ceramics for rebuilding bone, bridge bone defects or reshaping bone, and more particularly, to the combination of material properties and design to protect fractured portions or volumes to form new bone from movements and external forces that may interfere with bone formation and bone healing. This contributes to the best situation for natural bone healing, thereby improving clinical outcomes. The design of the implant member (1) is based on the initial bone structure (2) and the intended treatment, in which at least a portion of the bone-oriented inner surface (9) of the implant member (1) follows irregularities of the initial bone structure (2) to mechanically support the area around the fixation screws (6) for connecting the implant member to the initial bone structure (2).

The application discloses a kind of biocompatible materials and preparation method for bionic porous intervertebral fusion cage, belong to intervertebral fusion cage material technical field.A kind of biocompatible materials for bionic porous intervertebral fusion cage, by weight parts includes the following components: polyether ether ketone 65-80 parts, modified functional filler 15-25 parts, hydroxyapatite 8-12 parts, flow modifier 0.5-1 part;The modified functional filler is graphene oxide coated modified barium titanate functional filler.By introducing surface conductive modified nano piezoelectric ceramic particles, realize the effective conversion and transmission of mechanical stimulation to bioelectric signal, this microstructure design makes the insulating polyether ether ketone matrix possess the electric activity of simulating natural bone stress-electric signal response mechanism, so as to be favorable to activate osteoblast activity and promote bone integration of implant interface.

The invention discloses an interbody fusion cage based on a Thiessen polygonal gradient porous structure, and belongs to the technical field of biomedical engineering and orthopedic implants. The fusion cage body structure is of a three-dimensional communicated porous structure, the basic topological configuration of the porous structure is a Thiessen polygon, and the porous structure at least has gradient change in the distribution direction from the outer surface to the interior of the porous structure. The outer-layer small hole structure is beneficial for blood-source factors and osteoblasts to quickly enter and attach, so that osteogenesis is started; the inner-layer macroporous structure provides sufficient space for cell deep migration, blood vessel growth and new bone matrix deposition, sufficient osseointegration from outside to inside is finally achieved, the structure highly simulates a natural bone trabecula network, cell siphon attachment and internal connectivity are synergistically optimized through gradient pore design, and a specific post-treatment process is combined, so that the natural bone trabecula network structure is obtained. And the osseointegration performance and safety of the fusion cage are improved.

The invention belongs to the technical field of bionic bone scaffolds, and particularly relates to a TPMS structure bionic bone scaffold with an immune regulation function and a preparation method of the TPMS structure bionic bone scaffold. Comprising the following steps: preparing a three-period extremely-small curved surface bionic scaffold and micro-tissues, loading micro-tissue spheres in a methacryloyl gelatin solution, then pouring the micro-tissue spheres into the three-period extremely-small curved surface bionic scaffold, and carrying out photo-crosslinking gel forming to obtain the three-period extremely-small curved surface structure bionic bone scaffold with the immune regulation and control function. The bionic bone scaffold disclosed by the invention can be used for accurately simulating components and structures of natural bones, has good immunoregulation and osteogenesis promoting capabilities, and is expected to cooperate with functionalized micro-tissues to construct an immune microenvironment and promote bone tissue regeneration.

The invention provides a bioactive glass collagen composite material and a preparation method thereof. The composite material comprises bioactive glass and collagen, and the bioactive glass and the collagen are mixed together. The method comprises the following steps: 1) taking a collagen solution; 2) taking bioactive glass, and uniformly mixing the bioactive glass with neutral salt to obtain a mixture; 3) adding the mixture into a collagen solution in a stirring state to obtain a precipitate which contains bioactive glass, collagen and residual neutral salt; and 4) filtering out the precipitate, and fully washing to remove neutral salt to obtain the bioactive glass collagen composite material. The design is reasonable, the conception is ingenious, the composite material can form a structure similar to a natural bone tissue in a human body, and the bioactive glass and the collagen of the composite material prepared by the method are uniformly mixed.

The application relates to the technical field of natural high-molecular compound processing and medical materials, and discloses a method for preparing mineralized collagen based on a synchronous biomimetic calcification and siliconization strategy. The preparation method comprises the following steps: dissolving collagen in a buffer solution, adding a soluble calcium ion solution, adjusting the pH value to 8-10 under the condition of an ice water bath, then culturing under stirring and constant temperature conditions for 15-30 min; then adding a silicate and phosphate mixed solution dropwise under stirring, adjusting the pH value to 8-10, and then constant temperature culture for 12-24 h; and the mineralized collagen material prepared by separating the precipitate, washing with water and freeze-drying has synchronous biomimetic calcification and siliconization. The composite material constructed by the application has a high mineralization degree close to that of natural bone, exhibits good biocompatibility, can effectively promote the proliferation and differentiation of osteoblasts, and has a good application prospect in the field of bone repair and the like.

The application discloses an injectable and plastic composite bone powder material for dental implant and a preparation and application thereof, and comprises the following components in parts by mass: 1-5 parts of bone powder, 0.1-1 parts of decellularized extracellular matrix and 5 parts of calcium sulphate hemihydrate. The bone powder provides a porous three-dimensional structure with a natural bone structure and suitable new bone growth and bone inductivity. The decellularized extracellular matrix provides a three-dimensional framework containing extracellular matrix macromolecules, can promote rapid proliferation and adhesion of cells, and serves as a biomimetic mineralization template. The calcium sulphate hemihydrate provides good degradation time, injectability and in-situ plastic shaping and curing properties for the whole composite material. The three components are combined, so that the material is provided with good injectability and plasticity, and a biomimetic microenvironment with bone inductivity is constructed.

This invention belongs to the field of biological engineering technology, specifically relating to an MXene-based photothermal coupling composite film for bone repair and its preparation method; comprising MXene, type I collagen, and gelatin solution, obtained by sequential bridging and cross-linking with FeCl3 solution; wherein the mass ratio of MXene to type I collagen is (3-9):(1-7), and gelatin accounts for 3.6%-10.4% of the final composite material mass; the preparation method includes: ultrasonically dispersing MXene and mixing it with gelatin solution, then compounding it with type I collagen solution, vacuum filtering and drying to form a matrix film, and finally cross-linking it by impregnation with FeCl3 solution; this film has mechanical properties matching natural bone tissue, good biocompatibility, and high-efficiency photothermal conversion capability under near-infrared light irradiation, and is suitable for bone defect repair and photothermal synergistic therapy.

The present application relates to the technical field of medical magnesium alloy, and particularly relates to a medical magnesium alloy and a preparation method thereof. Although magnesium metal has similar elastic modulus and antibacterial performance to natural bone, its degradation speed in a physiological environment is too fast to hinder its clinical application. Based on the above problem, the present application provides a medical magnesium alloy containing Mg, Ga, Sr, Zn and other elements in its composition, which can not only reduce the degradation rate of the metal Mg in the physiological environment, but also significantly improve the antibacterial performance thereof, and is very beneficial to promoting the clinical application of the magnesium alloy.

The present application relates to the field of biomimetic mineralization and tissue engineering scaffold materials, and particularly relates to a kind of biomimetic mineralization silk fibroin scaffold material and its preparation method and application.The preparation method comprises the following steps:1) silk fibroin is induced self-assembly, and silk fibroin fiber matrix is obtained;2) the silk fibroin fiber obtained in step 1) is crosslinked, and crosslinked silk fibroin fiber mineralization matrix is obtained;3) the crosslinked silk fibroin fiber mineralization matrix obtained in step 2) is mineralized in mineralization solution, and biomimetic mineralization silk fibroin scaffold material is obtained.The biomimetic mineralization silk fibroin scaffold material prepared by the preparation method is applied to bone tissue repair.In the present application, the mineralization mode in silk fibroin fiber more highly simulates the mineralization form of natural bone tissue, and the similar surface chemical properties and nano-scale structure characteristics of natural bone are reproduced.

The invention discloses a preparation method of a medical titanium-based micro-arc oxidation hydroxyapatite-titanium dioxide coating. The method comprises the following steps: 1, preparing an electrolyte; and 2, carrying out micro-arc oxidation treatment on the titanium or the titanium alloy. According to the method, the titanium or the titanium alloy is used as an anode to be placed in the electrolyte for micro-arc oxidation treatment through a micro-arc oxidation method, hydroxyapatite and titanium dioxide are directly synthesized on the surface of the titanium or the titanium alloy through components in the electrolyte, and the hydroxyapatite-titanium dioxide coating is formed; the coating is mainly composed of hydroxyapatite, titanium dioxide and a small amount of amorphous compound, the content of hydroxyapatite in the coating is greatly increased, the matching degree with inorganic components of natural bones of a human body is high, and the coating has extremely high biocompatibility and safety; meanwhile, the micro-arc oxidation electrolyte is simple in component, the raw materials are easy to obtain, the price is low, the electrolyte does not contain Cr < 6 + > which has great harm to the human body and the environment, and the preparation method is suitable for industrial batch production.

The invention discloses a bone-periosteum bionic stent for vascularized bone regeneration and a preparation method of the bone-periosteum bionic stent, and belongs to the technical field of bone repair. The stent comprises a bone bionic stent and a bionic periosteum, the bone bionic scaffold is of a hierarchical pore structure and is formed by compounding polycaprolactone, silk fibroin and beta-tricalcium phosphate through fused deposition 3D printing, and the surface of the bone bionic scaffold is subjected to alkali treatment so as to improve hydrophilicity and osteogenic activity; the bionic periosteum is printed on the surface of the bone bionic scaffold through a near-field direct writing technology and is composed of polycaprolactone, mineralized collagen and magnesium ions, and the mineralized collagen is distributed in the bionic periosteum in a gradient mode. Through integrated bionic design, structures and functions of natural bones and periosteum are simulated; the bone bionic support plays a role in mechanical support and bone formation promotion; the bionic periosteum rapidly releases magnesium ions in the early stage of degradation, and early vascularization is promoted; along with degradation, continuously released collagen induces mineralization of a collagen matrix in the later period; the stent has good cell compatibility and capability of promoting osteogenic differentiation and blood vessel formation.

The invention relates to the technical field of high-performance polymer composite materials, and discloses a bionic skeleton material for a humanoid robot and a preparation method thereof.The method comprises the steps that 50-75 wt% of polyether-ether-ketone, 20-50 wt% of modified fibers and 1-3 wt% of an antioxidant are subjected to melt blending, extrusion and granulation, then vibration injection molding is conducted through an injection molding machine, the mold temperature ranges from 160 DEG C to 200 DEG C, and in the injection molding and pressure maintaining stage, the bionic skeleton material for the humanoid robot is obtained. And vibration shearing with the frequency range of 0.05-2 Hz is applied to the melt through reciprocating motion of the piston. According to the preparation method, flowing and crystallization of a polymer composite material melt in a cavity of an injection mold are regulated and controlled, so that internal fibers are promoted to be orderly arranged in the melt flowing direction, a polymer matrix is further induced to form a column crystal structure on the surface of the polymer matrix, a reinforcing structure similar to a natural bone is constructed in a bionic mode on the micro-scale, and the mechanical property of the composite material is remarkably improved.

The invention discloses a bionic polypeptide for inducing mineralization in type I collagenous fibers and application of the bionic polypeptide. The amino acid sequence of the novel NCPs bionic polypeptide comprises a collagen binding motif, a calcium ion chelation motif and a cell adhesion sequence; the amino acid sequence of the collagen binding motif comprises sequences as shown in SEQ ID NO. 1 to SEQ ID NO. 3. The novel NCPs bionic polypeptide disclosed by the invention can stabilize an amorphous calcium phosphate precursor and specifically bind to type I collagen, so that ordered intra-fiber mineralization is realized. The polypeptide disclosed by the invention breaks through the limitation of single function of a traditional non-collagen simulant, and provides a core technology for constructing a tissue engineering scaffold of which the structure and the performance are highly bionic to those of natural bones.

This invention discloses a highly efficient wood-based elastic artificial periosteum loaded with multiple osteogenic and angiogenic drugs, its preparation method, and its applications. The artificial periosteum uses wood-derived elastic wood as a base layer, polydopamine as a bonding layer, and layered drug-loaded electrostatic self-assembly layers as a biofunctional layer. The base layer not only mimics the structural characteristics and mechanical anisotropy of the natural periosteum, but its elasticity also facilitates clinical implantation of the artificial periosteum. The bonding layer effectively connects the base layer and the biofunctional layer, while also imparting certain adhesive properties to the artificial periosteum. The biofunctional layer can efficiently load multiple drugs, achieving synergistic and long-lasting promotion of osteogenic and angiogenic biological functions during bone repair. The artificial periosteum prepared by this invention possesses structural and mechanical anisotropy, high porosity, high elasticity, and adhesive properties; moreover, its drug loading and mechanical properties are controllable, making it an ideal novel artificial periosteum material.

The application discloses a design method of a bionic bone support based on TPMS and belongs to the technical field of bionic bone supports. The application is based on G unit and D unit formulas, adjusts a bias function to generate G gradient unit and D homogeneous unit CAD models; uses a fusion function to fuse the G gradient unit and the D homogeneous unit CAD models to generate a GD radial fusion CAD model, adjusts the bias function of the G gradient unit by using an interpolation method to make the GD radial fusion CAD model reach a target average porosity; reduces the wall thickness of the GD radial fusion CAD model to form a GD radial fusion CAD model with wall thickness; then divides the GD radial fusion CAD model with wall thickness into a finite element simulation grid model and outputs an inp format simulation model file; imports the inp format simulation model file into HyperMesh software, repairs and divides the grid and exports an inp file format model. The porous support designed by the application considers different functional grading of natural bone structures and different cell morphological fusion, better meets the mechanical properties of a natural femur, and light weight design increases the specific surface area to provide more space for bone cell adhesion and growth.

The invention discloses a demineralized bone matrix fiber as well as a preparation method and application thereof. The method comprises the following steps: firstly, cleaning and disinfecting natural bones separated out of a body, then cutting the natural bones into bone blocks, further degreasing the bone blocks, and cutting the bone blocks into fibers with the length of 1-100mm and the thickness of 0.2-1.4 mm; then placing the bone fibers obtained by cutting in an acidic demineralization solution for demineralization until the calcium content is 0-20%; the method comprises the following steps: demineralizing bone fibers, then reacting with the demineralized bone fibers by using an anionic surfactant for 0.5-3 hours, cleaning, then reacting for 12-18 hours by using a nonionic surfactant, completing two-stage decellularization, cleaning, and freeze-drying for preservation. During clinical use, the demineralized bone fibers which are kept in a freeze-drying manner are mixed with hydrated liquid or mixed with one of sodium carboxymethyl cellulose, gelatin, poloxamer and chitosan to form an ointment substance which is used as a bone defect repairing material.

The invention discloses a biomimetic mineralized hydrogel, which is characterized in that the biomimetic mineralized hydrogel is prepared from a cross-linked hydrogel matrix, mesoporous bioactive glass and an active polypeptide P148, wherein the cross-linked hydrogel matrix is cross-linked methylacryloylated gelatin, and the cross-linked hydrogel matrix is cross-linked methylacryloylated gelatin; the mesoporous bioactive glass is uniformly distributed in the cross-linked hydrogel matrix, and the active polypeptide P148 is loaded on the mesoporous bioactive glass. The invention further provides a preparation method of the biomimetic mineralized hydrogel and application of the biomimetic mineralized hydrogel in preparation of bone defect repair materials. According to the biomimetic mineralized hydrogel disclosed by the invention, a natural bone matrix is synergistically simulated from chemical components and a multilevel structure, a dual-action mechanism of mineralization induction and cell differentiation is realized, and an efficient and ordered bone regeneration solution is provided for critical-size bone defects.

The application discloses a high-strength silk nanofiber reinforced composite membrane and a preparation method thereof. The composite membrane is obtained by laminating and hot-pressing after freeze-drying of a plurality of layers of silk nanofiber reinforced hydrogels. The silk nanofiber reinforced hydrogel is composed of a hydrogel matrix and silk nanofibers and amorphous calcium phosphate particles uniformly distributed in the hydrogel matrix. The hydrogel matrix is a double-network hydrogel formed by cross-linking of silk fibroin and acylated chitosan respectively. The silk nanofiber reinforced composite membrane provided by the application has excellent mechanical properties, meets the mechanical requirements of non-load-bearing parts, can effectively promote the repair of bone defects, and has a hierarchical structure of natural bone, which provides an ideal microenvironment for cell adhesion, proliferation and migration.

The invention discloses a biocompatible material for a bionic porous interbody fusion cage and a preparation method, and belongs to the technical field of interbody fusion cage materials. The invention discloses a biocompatible material for a bionic porous interbody fusion cage. The biocompatible material is prepared from the following components in parts by weight: 65 to 80 parts of polyether-ether-ketone, 15 to 25 parts of modified functional filler, 8 to 12 parts of hydroxyapatite and 0.5 to 1 part of flow modifier, the modified functional filler is a graphene oxide coated modified barium titanate functional filler. By introducing the nano piezoelectric ceramic particles subjected to surface conductive modification, effective conversion and transmission from mechanical stimulation to bioelectrical signals are realized, and the microstructure design enables an insulated polyether-ether-ketone matrix to have electrical activity for simulating a natural skeleton stress-electrical signal response mechanism; therefore, the activity of osteoblasts can be activated, and osseointegration of bones and an implant interface can be promoted.

This invention discloses a bio-barrier membrane with guided bone regeneration (GBR) function and its construction method, belonging to the technical field of bone tissue regeneration and repair materials. The bio-barrier membrane is a composite structure formed by a decellularized periosteum and an extracellular matrix (ECM) cross-linked layer. Specifically, it is prepared by in-situ cross-linking of the decellularized periosteum with an acidic ECM solution. This bio-barrier membrane is a bilayer composite membrane that mimics the natural periosteum, synergistically exerting barrier, osteoconduction, and osteoinduction effects, significantly improving bone repair and regeneration.