106 results about "Bone scaffold" patented technology

The invention provides an interface-enhanced regenerated bone scaffold for sustained-release medicines as well as a preparation method and application of the interface-enhanced regenerated bone scaffold, and belongs to the technical field of bone tissue engineering. According to the invention, polycaprolactone and hydroxyapatite are taken as matrix components of the regenerated bone scaffold, so that the regenerated bone scaffold has mechanical strength matched with a host bone and good biocompatibility; the polydopamine coating is loaded on the surface and internal pores of the regenerated bone scaffold matrix, surface functional modification is easy to perform, and a catechol structure in the polydopamine coating can perform coordination binding with metal ions in MOFs, so that the falling rate of MOFs particles is reduced, and the MOFs particles are uniformly and stably distributed; the MOFs particles are used for loading the eucommia ulmoides extract, safety and non-toxicity are achieved, the eucommia ulmoides extract is encapsulated in the MOFs particles, the stability of the eucommia ulmoides extract can be improved, and a better drug release effect and a better bone regeneration promoting effect are achieved.

The invention provides a design method of a personalized gradient porous bone scaffold based on patient apparent bone mineral density. The design method comprises the following steps: constructing a variable density gradient porous structure of the bone scaffold; obtaining sample data through compression mechanical simulation and fluid analogue simulation; constructing a BP neural network model; the apparent bone mineral density of a patient is obtained through detection, the theoretical elastic modulus is calculated, and the mechanical-permeability coordinated gradient bone scaffold structure parameters are predicted through a BP neural network model; and an intelligent platform for designing a personalized gradient porous bone scaffold structure is built. The method has the beneficial effects that the gradient porous structure is constructed by controlling the offset in the TPMS function, and the bone scaffold gradient porous structure which is matched with the apparent bone density of a patient and is coordinated in mechanical and penetrating performance is realized through performance simulation and neural network data processing; and a bone scaffold parameter prediction platform is established to provide a personalized bone scaffold structure design scheme for convenient and efficient bone repair medical treatment.

The invention discloses a bone scaffold fusion design and preparation method based on mirror image registration, and the method comprises the steps: firstly, based on a three-dimensional medical image of a femoral defect region of a patient, combining an opposite-side complete femoral CT image, and achieving the automatic recognition and complementation of the defect region through the mirror image transformation and ICP registration technology; after defect boundary extraction is completed, three-dimensional reconstruction is carried out on the individualized outer contour of the left bone tissue, and an STL format model is exported. According to the femur functional partition characteristics, a Diamond structure is adopted in a cortical bone area to enhance mechanical support, and a Gyandroid structure is selected in a cancellous bone area to achieve porosity gradient filling, so that the permeability is optimized, and cell proliferation is promoted. Continuous transition among the structures is realized through a Sigmoid function, and the stability and the biological adaptability of the whole structure are improved. And finally, the stent model is subjected to selective laser melting, and a Zn-3Mg alloy material is utilized to complete printing manufacturing.

The invention discloses a 3D printing self-adaptive control system for bone defect, and relates to the technical field of 3D printing self-adaptive control, a miniaturized optical coherence tomography, a high-resolution camera, a force sense and temperature sensor and the like are installed on an operation site, and the whole process of material deposition and solidification is monitored; then, deviation is rapidly recognized through an anomaly detection and error prediction model, defect positions are output, and a closed-loop control unit is combined with model prediction control and self-adaptive path planning to achieve online adjustment of key variables such as the extrusion amount and the printing speed; when the defect cannot be made up automatically through parameter adjustment, the system can trigger local shutdown and secondary repair, the forming quality is evaluated through multi-modal scanning and a repair scoring function after repair, and the high matching degree and mechanical stability of the final bone scaffold and the bone defect part are ensured; the cooperation of multi-modal sensing and local path planning enables the system to have higher adaptability.

The invention discloses a light-driven / temperature-driven double-trigger shape memory antibacterial and anti-tumor bone scaffold and a fused deposition modeling (FDM) preparation method thereof, and is characterized in that polylactic acid (PLA) is used as a matrix, and polyethylene glycol (PEG) and nano magnesium hydroxide (Mg (OH) 2) / copper oxide (CuO) are uniformly dispersed through an organic solvent blending method; a composite wire is prepared through the processes of drying and film forming, crushing and granulation and screw extrusion, and finally, the integrated molding of the three-dimensional porous scaffold is realized through an FDM (fused deposition modeling) process. According to the stent, temperature and near-infrared light double-triggered shape memory is achieved through cooperation of glass transition regulation and control of PLA-PEG and the photothermal effect of the nano metal oxide particles. Nano Mg (OH) 2 / CuO endows the scaffold with multi-mode antibacterial and anti-tumor functions: 1) Mg < 2 + > / Cu < 2 + > releases and destroys microbial membrane potential; according to the stent, the limitation of shape fixation of a traditional implant is broken through, shape self-adaption can be triggered through light / heat stimulation, irregular bone defects can be accurately attached, the risk of a secondary operation is avoided, and the stent has remarkable advantages in minimally invasive orthopedic repair.

The invention discloses a bone immunoregulation osteogenesis promoting method of PLL-MgBGs / SF frozen gel and an osteogenesis capability detection method of the PLL-MgBGs / SF frozen gel. The bone immunoregulation osteogenesis promoting method comprises the following steps: preparing a magnesium-doped bioactive substance MgBGs; the preparation method comprises the following steps: preparing PLL-MgBGs nano particles; pLL-MgBGs / SF frozen gel is prepared; a sterile PLL-MgBGs / SF frozen gel leaching solution is prepared; the method comprises the following steps: inoculating macrophages (RAW264.7) into a 48-pore plate, culturing by using a complete culture medium containing LPS (100 ng / mL), and then culturing by using a complete culture medium containing a PLL-MgBGs / SF frozen gel leaching solution. The PLL-MgBGs / SF freezing gel has the advantages that the prepared PLL-MgBGs / SF freezing gel is excellent in water response shape memory ability, capable of releasing various active ions such as Mg < 2 + >, Ca < 2 + > and SiO4 < 4->, remarkable in potential in immunoregulation and bone repair, capable of being implanted into bone defects in an injection mode to fill the bone defects, capable of being rapidly matched with irregular bone defects and capable of improving the bone repair effect. According to the present invention, the problem of insufficient matching of the traditional bone scaffold and the irregular defect site is solved, the excellent mechanical stability and the excellent structure retention can be represented during the degradation process, the good supporting ability is provided in the bone defect region, the polarization of the macrophage can be adjusted to be the M2 type, and the collection, the proliferation and the osteogenic differentiation of the bone marrow mesenchymal stem cells (BMSCs) can be promoted.

The invention relates to the technical field of bone tissue engineering and regenerative medicine, and discloses a stem cell-loaded bionic bone scaffold material, which comprises a bone scaffold main body, the bone scaffold main body is formed by compounding hydroxyapatite and a biodegradable high-molecular polymer and has a porous structure, the porosity is 70%-90%, the aperture range is 100-500 microns, and pores are communicated with one another; a vascularization induction factor slow release system is arranged in the porous structure of the bone scaffold main body, the stem cell-loaded bionic bone scaffold material does not need to take the autologous bone of a patient, and the problems of donor injury and limited sources are avoided; the composite scaffold is matched with low-immunogenicity stem cells, so that the risks of immunological rejection and disease transmission are avoided; the material has the advantages of good biocompatibility, mechanical matching with human bones, degradability, no secondary operation, promotion of vascular regeneration and stem cell osteogenesis, and realization of bone regeneration integration.

According to the method for designing the biological bone scaffold of the tetragonal symmetric lattice structure based on the minimal curved surface, reconstruction is performed based on a classic three-period minimal curved surface lattice structure implicit function for the biological bone scaffold, and a novel implicit function of the three-period minimal curved surface lattice structure is obtained; and judging whether a lattice structure obtained on the basis of the reconstructed implicit function meets a set constraint condition or not, and if so, designing and preparing the biological bone scaffold by using the reconstructed implicit function. Therefore, the biological bone scaffold prepared from the tetragonal symmetrical lattice structure has good mechanical properties and material transport performance on the premise of light weight.

The application discloses a lateral flow fishbone-shaped inclined plate assembly and an inclined plate sedimentation tank. The inclined plate assembly comprises a fishbone support, an inclined plate connecting piece and an inclined plate. The inclined plate is in the form of a flat plate. The mounting portion of the inclined plate connecting piece is in the form of a long and thin plate and is arranged in an inclined manner. The upper end of the inclined plate is mounted on the mounting portion. An extension portion is arranged on the lower side of the mounting portion. A washing assembly for washing the inclined plate is mounted on the lower end of the extension portion. The fishbone support is arranged in a vertical arrangement. A plurality of pairs of inclined plate connecting pieces are arranged along the length direction of the fishbone support. Each pair of inclined plate connecting pieces is oppositely arranged on the two sides of the fishbone support. Each pair of inclined plate connecting pieces is connected with the fishbone support through the connecting portion thereof. The lower end of the mounting portion is inclined towards the direction away from the fishbone support. The application adopts a unique inclined plate structure and a fishbone-shaped inclined plate assembly, and is provided with a washing assembly for effectively washing the inclined plate. The application effectively increases the allowable length of the inclined plate while ensuring the structural stability of the inclined plate assembly and the inclined plate module, reduces the maintenance interval, and improves the sedimentation effect.

The invention relates to the technical field of bone defect repair, in particular to a composite cylindrical bionic bone scaffold and a preparation method thereof.The preparation method comprises the following steps of regenerated material preparation, ZP scaffold preparation, composite hydrogel preparation, PH scaffold preparation and scaffold assembling, specifically, the ZP scaffold and the PH scaffold are subjected to ultraviolet disinfection treatment, then a phosphate buffer solution is used for flushing treatment, and the composite cylindrical bionic bone scaffold is obtained; the preparation method comprises the following steps: implanting endothelial cells into a PH scaffold according to 1 * 10 < 5 > mL, carrying out perfusion culture for 6-8 days, and combining a ZP scaffold with the PH scaffold in which the endothelial cells are implanted to form the macro-microstructure composite cylindrical bionic bone scaffold. According to the composite cylindrical bionic bone scaffold and the preparation method thereof, the ZP scaffold and the PH scaffold are combined to form the vascularized composite cylindrical bionic bone scaffold containing internal and external bionics, so that the composite cylindrical bionic bone scaffold has satisfactory biocompatibility, and the angiogenesis and osteogenesis processes are obviously promoted.

The invention relates to the technical field of cancellous bone scaffolds, and discloses a topological optimization-based cancellous bone scaffold reverse generation model determination method and device, in the method, a three-dimensional thin layer structure is subjected to topological optimization processing, and complex and irregular microstructure characteristics of cancellous bone tissues can be simulated. The first multi-channel mechanical nephogram and the second multi-channel mechanical nephogram are force-related features. And multi-modal data composed of the three-dimensional thin-layer structure, the initial simplified model, the first multi-channel mechanical cloud picture and the second multi-channel mechanical cloud picture is used as a training sample to train a potential map diffusion model, so that the model can obtain strong performance of outputting a cancellous bone scaffold result under the training of a multi-modal input condition.

The application provides a alginate-based aerogel, a preparation method and application thereof. The alginate-based aerogel has parallelly arranged large pores and oriented channels at an angle with the large pores, and the angle ranges from 0 to 180 degrees. The large pores are distributed in the same direction inside the alginate-based aerogel, and the oriented channels improve the interconnectivity between the large pores. The existence of the large pores and the oriented channels makes the alginate-based aerogel have the characteristics of anisotropy and a multi-scale pore structure, and the size of the large pores and the oriented channels is suitable for inducing vascularization and osteogenesis. Therefore, the aerogel can be used as a bone scaffold material. Tests show that the aerogel has the characteristics of light weight and high strength. In addition, other components beneficial to osteogenesis can be added in the alginate system, and the size of the pores can be adjusted without affecting the formation of the pore structure.

The invention discloses a bionic bone scaffold composite material prepared through combination of electrostatic spinning and 3D printing and a preparation method of the bionic bone scaffold composite material, and relates to the technical field of bionic bone scaffold composite materials. A bionic bone scaffold composite material prepared by combination of electrostatic spinning and 3D printing is characterized by comprising 80 to 100 parts of a biodegradable high-molecular polymer, 5 to 30 parts of a bioactive inorganic ceramic material, 200 to 500 parts of a solvent, 0.01 to 5 parts of a bioactive factor, 0.1 to 0.5 part of a bacteriostatic active compound and 0.01 to 5 parts of naringin. According to the invention, the metronidazole derivative is used for providing antibacterial ability, and is matched with naringin to realize anti-inflammatory and osteogenesis-promoting synergistic effect, so that the problem of drug resistance or non-uniform drug release of the traditional antibacterial means is avoided, the induction effect of the stent on bone regeneration is enhanced, and the infection risk in the repair process is reduced.

The invention relates to the technical field of bone scaffold materials, and discloses a preparation method and application of a pre-vascularized bone repair scaffold, the preparation method of the pre-vascularized bone repair scaffold comprises the following steps: firstly, obtaining a porous scaffold and carrying out sulfonation treatment to obtain a sulfonated scaffold; then preparing a mixed solution containing gelatin and an extracellular matrix, and adding mesenchymal stem cells and / or endothelial cells to obtain a cell mixed preparation; and finally, contacting the cell mixed preparation with the sulfonated scaffold, and culturing to obtain the sulfonated scaffold. The pre-vascularized bone repair scaffold prepared by the preparation method disclosed by the invention simultaneously has excellent angiogenesis promoting capability and osteogenesis induction characteristic in vitro, and realizes vascularization-ossification synergistic interaction through a positive feedback loop mediated by a paracrine signal network; and a new thought and a new method are expected to be developed for clinical application of large-segment bone defect repair.

The application discloses a kind of biomimetic silk fibroin cartilage scaffolds for cranium defect repair, belong to biomedical engineering technical field.To solve the problem of single structure, low bone formation efficiency and mismatching of mechanical properties and degradation rate of existing cranium repair scaffold, the application provides a kind of scaffold, which includes in order from outside to inside: biomimetic periosteum layer, bone conduction and vascularization layer and cartilage induction layer.Biomimetic periosteum layer is dense nanofiber membrane structure, and plays the role of physical barrier and osteoinduction;Bone conduction and vascularization layer is gradient porous structure with gradually decreasing aperture from outside to inside, aiming to guide the ordered growth of blood vessels and cells, and promote rapid vascularization;Cartilage induction layer is sponge-like microporous structure, used for forming stable cartilage template and starting endochondral ossification.The application simulates the natural bone healing process through structural biomimicry and functional zoning, synergistically promotes vascularization and osteogenesis, and realizes efficient biological repair of cranium defect.

The utility model discloses a 3D printing hydrogel cartilage scaffold performance detection device, and relates to the technical field of hydrogel cartilage scaffold performance detection, the 3D printing hydrogel cartilage scaffold performance detection device comprises a performance detection device main body, the top of the performance detection device main body is uniformly provided with three storage grooves, a silica gel block is detachably mounted at the bottom of the inner wall of a storage groove formed in the performance detection device body, a working plate is detachably mounted at the top of the silica gel block, and a hydraulic push rod is detachably mounted on one side of the top of the working plate. According to the 3D printing hydrogel cartilage scaffold performance detection device, the arranged clamping plates drive the silica gel pads to be matched with the clamping seats to clamp to-be-detected printing hydrogel cartilage scaffolds of different sizes, the application range is wider, and the practicability is higher; the 3D printing hydrogel cartilage scaffold is fully detected by utilizing the reciprocating motion of the hydraulic push rod, the detection result is more reliable, and the hydraulic push rod on the working plate is damped through the cooperation of the arranged silica gel block and the fixed socket and the insertion rod.

The invention discloses a preparation method of porous ceramic with a non-uniform fusion structure, which comprises the following steps: step 1, constructing a fusion function, and determining parameters of the fusion function; 2, preparing a wax pattern negative template; step 3, preparing ceramic slurry; step 4, dipping and freezing; step 5, low-pressure drying; and 6, sintering to obtain the TPMS structure porous ceramic. The invention further discloses the porous ceramic with the non-uniform fusion structure. The invention further discloses application of the porous ceramic with the non-uniform fusion structure in the field of bionic bone scaffolds. According to the method, the fusion function is constructed by fusing the G-shaped function and the TPMS function, so that non-uniform fusion of various hole patterns is realized; the mode of wax negative template freezing casting and indirect forming is adopted, the design freedom degree of a complex structure is reserved, meanwhile, the problems of resolution limitation, shrinkage deformation, supporting difficulty and the like during direct 3D printing of the porous ceramic are solved, and the use effect is good.

The present invention provides an integrated design and manufacturing technology for a bone cartilage biomimetic gradient scaffold. The invention includes a functionally graded integrated bone cartilage biomimetic gradient scaffold, a transition structure for achieving graded transition between hard bone and cartilage tissue, and a biomimetic cartilage formation and post-processing method. The integrated bone cartilage biomimetic gradient scaffold is a porous interconnected ceramic structure that can be manufactured through integrated additive manufacturing. The transition structure is a special structure for firmly connecting hard bone and cartilage. The biomimetic cartilage formation and post-processing method involves performing special treatment on the 3D-printed bone scaffold to form biomimetic cartilage, thereby improving the strength of the cartilage layer and preventing cartilage damage caused by joint friction. The bone cartilage biomimetic scaffold structure proposed by the present invention has effective functional grading to mimic natural bone cartilage tissue, which is beneficial for preventing stress shielding and can be manufactured in one piece using 3D additive manufacturing technology.

The invention discloses a preparation method and application of a functional degradable soybean oil-based bionic porous composite bone scaffold. The method comprises the following steps: taking epoxidized soybean oil acrylate as a bio-based base material, compounding isobornyl methacrylate to improve the defects of high viscosity and low photosensitivity of the bio-based base material, adding a photoinitiator containing ethyl 2, 4, 6-trimethylbenzoyl phenyl phosphonate and phenyl bis (2, 4, 6-trimethylbenzoyl) phosphine oxide, compounding, and carrying out aging treatment to obtain base resin; mixing calcium lignosulphonate into the base resin to prepare composite photosensitive resin, and printing a triple-period extremely-small curved surface structure through a digital light processing technology and a 3D printing technology to finally obtain the functional degradable soybean oil-based bionic porous scaffold. The scaffold has calcium lignosulphonate-mediated controllable photo-thermal response behaviors of near-infrared light illumination regulation and bone scaffold component dependence under the condition of simulating the in-vivo environment of a human body, so that a controllable photo-thermal response-mediated remote shape memory function is realized, and meanwhile, a mild, safe and controllable photo-thermal curative effect can also be realized. Besides, calcium lignosulphonate promotes degradation of the composite scaffold by preferentially dissolving and generating a microporous structure on the surface of a matrix while improving the hydrophilic performance of the scaffold, and continuously releases calcium ions, so that the scaffold shows enhanced biomineralization ability and promotes cell proliferation and osteogenic differentiation. The research provides a solution with potential for the soybean oil-based biological photosensitive material in the digital light processing preparation technology and the multi-functional application of the bionic porous bone scaffold so as to deal with complex and irregular bone defects.

The application discloses a preparation method of non-uniform fusion structure porous ceramic, comprising the following steps: step one, constructing a fusion function and determining parameters of the fusion function; step two, preparing a wax negative template; step three, configuring ceramic slurry; step four, impregnating and freezing; step five, low-pressure drying; and step six, sintering, so as to obtain TPMS structure porous ceramic. The application also comprises non-uniform fusion structure porous ceramic. The application also comprises application of the non-uniform fusion structure porous ceramic in the field of biomimetic bone scaffolds. The application constructs a fusion function by fusing a G-shaped function and a TPMS function, realizes non-uniform fusion of various pore types, adopts a wax negative template freezing casting and an indirect forming mode, retains the design freedom of a complex structure, solves problems such as resolution limitation, shrinkage deformation and support difficulty that are faced by direct 3D printing of porous ceramic, and has good use effect.

The invention discloses a model construction method of a gradient random porous structure of a simulated bone trabecula, a white calendite piezoelectric bone scaffold and a preparation method of the white calendite piezoelectric bone scaffold, and the model construction method provided by the invention is a design method based on a Thiessen polygon porous structure with seed point release gradient change and volume scaling coefficient linear gradient change. In the body center scaling process, interpolation calculation is conducted on scaling coefficients from the axis to the outermost end in the cylindrical domain in the radial direction, and therefore the model of the stochastic gradient porous structure with the pore diameter decreasing from inside to outside in the radial direction and the pore edge diameter increasing from inside to outside in the radial direction is finally formed, by designing the formula and process parameters of the white calcium stone ceramic slurry, the possible problems of over-curing, cracking and the like are avoided, and the white calcium stone piezoelectric ceramic bone scaffold with excellent bonding performance is prepared by adopting a 3D printing photocuring printing technology.

The invention provides an ink composition. The ink composition is prepared from drug carrying microspheres, hydroxyapatite, sodium alginate and carrageenan. The ink material disclosed by the invention has good shear thinning performance, rheological property, stability and self-healing capability. The stent provided by the invention has good mechanical strength and hydrophilic performance. The stent provided by the invention is safe and has no cytotoxicity. And the NGF is fused into the bone scaffold, so that local continuous release can be realized, and the effect on the repaired part is lasting and effective.

The application claims a preparation method of a ceramic / polymer continuous gradient porous scaffold, and belongs to the technical field of biological tissue engineering bone scaffold. The porous scaffold structure adopts a three-period minimal surface (TPMS) design, and the scaffold structure and volume fraction are controllable. The continuous gradient material adopts a mixed slurry of different volume fractions of hydroxyapatite powder and photosensitive PLA resin as the material, and a gradient polylactic acid layer containing 0% to 30% of nano-hydroxyapatite is sequentially arranged from inside to outside. The high volume fraction of HA / PLA on the outside promotes cell adhesion and improves the bone ingrowth ability. The pure PLA scaffold is prepared by a 3D printing stereolithography forming method, the scaffold surface is activated by using a plasma cleaning process, the slurry with the ceramic material composition on the outer layer is attached to the scaffold surface by using a solution immersion method, the excess slurry in the pores is removed by using a centrifugal device, and ultraviolet light (wavelength range 360-410 nm) is used for irradiation and curing. The application uses a continuous gradient to overcome the shortcoming of the sharp change of the performance of the traditional composite material at the material interface, which leads to stratification.

The application discloses a SrCO3@ZIF-8 / polymer composite bone scaffold and a preparation method thereof, and comprises the following steps: growing zeolite imidazolate framework-8 (ZIF-8) on the surface of SrCO3 in situ to prepare SrCO3@ZIF-8 microrods, uniformly mixing the SrCO3@ZIF-8 microrods and a polymer material through grinding, and preparing the SrCO3@ZIF-8 / polymer composite bone scaffold by using a selective laser sintering technology. The SrCO3@ZIF-8 / polymer composite bone scaffold obtained by the application is applied to bone defect repair, and the Zn ions and Sr ions generated by in-vivo degradation of the scaffold can promote the differentiation of stem cells into osteoblasts, endow the scaffold with osteogenic activity, promote the polarization of macrophages into M2 type and the expression of related anti-inflammatory factors, and strengthen the anti-inflammatory performance of the scaffold. In addition, the ZIF-8 contains a large number of 2-methyl imidazole organic ligands, can form a strong interface with the molecular chain of the polymer, solves the problem of interface incompatibility between SrCO3 and the polymer, and thus improves the mechanical performance of the scaffold.

The invention provides a piezoelectric composite material compositely integrated by high-performance piezoelectric conductive hydrogel and a biphase bone scaffold. The piezoelectric composite material is formed by compositing piezoelectric hydrogel, conductive hydrogel and a tricalcium phosphate / hydroxyapatite biphase scaffold, the piezoelectric hydrogel is formed by wrapping glycine nanofibers with a modified polymer; the conductive hydrogel is formed by complexing a polymer solution containing lithium acetate, borate ions and a hydroxyl compound. The pressure-responsive self-generating composite material which is high in conductivity, high in voltage, high in support and biodegradable is formed by integrating the pressure-conductive hydrogel and the biphase bone scaffold, construction of a composite hydrogel system which is high in voltage, high in conductivity, high in support strength and biodegradable is achieved, and the application requirement of a high-performance bone repair material system is met.

The invention discloses a collagen and hydroxyapatite composite bone scaffold as well as a preparation method and application thereof, and relates to the technical field of bone scaffolds. The preparation method of the collagen and hydroxyapatite composite bone scaffold comprises the following steps: dissolving collagen in an acid solution to obtain a collagen solution with the concentration of 10-80 mg / L, mixing the collagen solution with hydroxyapatite, and stirring to obtain a compound; freeze-drying the compound, and cross-linking to obtain a primary product; washing the primary product with water to remove acid residues, and then freeze-drying to obtain the collagen and hydroxyapatite composite bone scaffold. The composite bone scaffold is spongy, has relatively high porosity, is beneficial to bone conduction and bone induction, is not easy to disintegrate and slag when being wetted, and is relatively good in supporting property.

The invention belongs to the technical field of bionic bone scaffolds, and particularly relates to a TPMS structure bionic bone scaffold with an immune regulation function and a preparation method of the TPMS structure bionic bone scaffold. Comprising the following steps: preparing a three-period extremely-small curved surface bionic scaffold and micro-tissues, loading micro-tissue spheres in a methacryloyl gelatin solution, then pouring the micro-tissue spheres into the three-period extremely-small curved surface bionic scaffold, and carrying out photo-crosslinking gel forming to obtain the three-period extremely-small curved surface structure bionic bone scaffold with the immune regulation and control function. The bionic bone scaffold disclosed by the invention can be used for accurately simulating components and structures of natural bones, has good immunoregulation and osteogenesis promoting capabilities, and is expected to cooperate with functionalized micro-tissues to construct an immune microenvironment and promote bone tissue regeneration.

A method for characterizing a bone tissue scaffold produced by an additive manufacturing process is provided. An image of the bone tissue scaffold is first captured and a boundary of the bone tissue scaffold is determined. One or more surface features of the bone tissue scaffold are subsequently detected using an edge detection algorithm, with the one or more surface features including one or more pores and / or one or more highlights. Edges that correspond to a pore using a pore filling algorithm are identified and the surface porosity of the bone tissue scaffold is thereby mapped. Systems for characterizing a bone tissue scaffold are also provided and make use of an image capture device for capturing an image of the bone tissue scaffold, as well as a processor for analyzing the image via instructions stored on the processor.

The present invention discloses a photothermal bone tumor scaffold with controlled drug release, as well as its preparation method and application. The scaffold has two sets of curved, independently connected channels, with the outer channel being open and connected, and the inner channel being closed and connected. The scaffold is made of a Ca2MgSi2O7 / ZTA composite ceramic material. The preparation process involves adding ceramic slurry to a photocuring printing device, performing photocuring printing based on a photothermal bone tumor scaffold model to produce a ceramic blank, performing secondary curing on the blank to produce a ceramic green body, and sintering the green body to obtain the photothermal bone tumor scaffold. The scaffold provided by the present invention has high porosity and strength, possesses an independent dual-channel structure, and integrates multiple functions: osteogenesis, controlled release of chemotherapy drugs, and photothermal killing of bone tumor cells.

The invention provides a cell-loaded bionic bone scaffold with oxygen self-production and bacteriostasis effects as well as a preparation method and application thereof, and belongs to the technical field of bone tissue engineering. The bionic bone scaffold provided by the invention is formed by stacking and assembling a plurality of cylindrical bone scaffold units, has a natural bone simulated Hauses tube structure, has good biocompatibility and excellent mechanical properties, and can accurately simulate the pore and structural characteristics of a natural bone. The functional GelMA hydrogel filled in the grooves is loaded with different cells, biological factors and antibacterial drugs, so that osteogenesis and vascularization effects at specific positions of the stent are realized, formation of a vascularization network and bone repair by simulating a natural bone structure of a human body are facilitated, and a good antibacterial effect is achieved. According to the invention, the cylindrical bone scaffold unit is loaded with the self-oxygen-producing MOFs particles, and the self-oxygen-producing effect of slowly releasing oxygen can be achieved after the cylindrical bone scaffold unit is implanted in vivo, so that bone marrow mesenchymal stem cell osteogenic differentiation and vascularization network construction are promoted.