50 results about "Bone scaffold" patented technology

The invention relates to the technical field of bone tissue engineering and regenerative medicine, and discloses a stem cell-loaded bionic bone scaffold material, which comprises a bone scaffold main body, the bone scaffold main body is formed by compounding hydroxyapatite and a biodegradable high-molecular polymer and has a porous structure, the porosity is 70%-90%, the aperture range is 100-500 microns, and pores are communicated with one another; a vascularization induction factor slow release system is arranged in the porous structure of the bone scaffold main body, the stem cell-loaded bionic bone scaffold material does not need to take the autologous bone of a patient, and the problems of donor injury and limited sources are avoided; the composite scaffold is matched with low-immunogenicity stem cells, so that the risks of immunological rejection and disease transmission are avoided; the material has the advantages of good biocompatibility, mechanical matching with human bones, degradability, no secondary operation, promotion of vascular regeneration and stem cell osteogenesis, and realization of bone regeneration integration.

The application discloses a lateral flow fishbone-shaped inclined plate assembly and an inclined plate sedimentation tank. The inclined plate assembly comprises a fishbone support, an inclined plate connecting piece and an inclined plate. The inclined plate is in the form of a flat plate. The mounting portion of the inclined plate connecting piece is in the form of a long and thin plate and is arranged in an inclined manner. The upper end of the inclined plate is mounted on the mounting portion. An extension portion is arranged on the lower side of the mounting portion. A washing assembly for washing the inclined plate is mounted on the lower end of the extension portion. The fishbone support is arranged in a vertical arrangement. A plurality of pairs of inclined plate connecting pieces are arranged along the length direction of the fishbone support. Each pair of inclined plate connecting pieces is oppositely arranged on the two sides of the fishbone support. Each pair of inclined plate connecting pieces is connected with the fishbone support through the connecting portion thereof. The lower end of the mounting portion is inclined towards the direction away from the fishbone support. The application adopts a unique inclined plate structure and a fishbone-shaped inclined plate assembly, and is provided with a washing assembly for effectively washing the inclined plate. The application effectively increases the allowable length of the inclined plate while ensuring the structural stability of the inclined plate assembly and the inclined plate module, reduces the maintenance interval, and improves the sedimentation effect.

The application provides a alginate-based aerogel, a preparation method and application thereof. The alginate-based aerogel has parallelly arranged large pores and oriented channels at an angle with the large pores, and the angle ranges from 0 to 180 degrees. The large pores are distributed in the same direction inside the alginate-based aerogel, and the oriented channels improve the interconnectivity between the large pores. The existence of the large pores and the oriented channels makes the alginate-based aerogel have the characteristics of anisotropy and a multi-scale pore structure, and the size of the large pores and the oriented channels is suitable for inducing vascularization and osteogenesis. Therefore, the aerogel can be used as a bone scaffold material. Tests show that the aerogel has the characteristics of light weight and high strength. In addition, other components beneficial to osteogenesis can be added in the alginate system, and the size of the pores can be adjusted without affecting the formation of the pore structure.

The application discloses a kind of biomimetic silk fibroin cartilage scaffolds for cranium defect repair, belong to biomedical engineering technical field.To solve the problem of single structure, low bone formation efficiency and mismatching of mechanical properties and degradation rate of existing cranium repair scaffold, the application provides a kind of scaffold, which includes in order from outside to inside: biomimetic periosteum layer, bone conduction and vascularization layer and cartilage induction layer.Biomimetic periosteum layer is dense nanofiber membrane structure, and plays the role of physical barrier and osteoinduction;Bone conduction and vascularization layer is gradient porous structure with gradually decreasing aperture from outside to inside, aiming to guide the ordered growth of blood vessels and cells, and promote rapid vascularization;Cartilage induction layer is sponge-like microporous structure, used for forming stable cartilage template and starting endochondral ossification.The application simulates the natural bone healing process through structural biomimicry and functional zoning, synergistically promotes vascularization and osteogenesis, and realizes efficient biological repair of cranium defect.

The application discloses a preparation method of non-uniform fusion structure porous ceramic, comprising the following steps: step one, constructing a fusion function and determining parameters of the fusion function; step two, preparing a wax negative template; step three, configuring ceramic slurry; step four, impregnating and freezing; step five, low-pressure drying; and step six, sintering, so as to obtain TPMS structure porous ceramic. The application also comprises non-uniform fusion structure porous ceramic. The application also comprises application of the non-uniform fusion structure porous ceramic in the field of biomimetic bone scaffolds. The application constructs a fusion function by fusing a G-shaped function and a TPMS function, realizes non-uniform fusion of various pore types, adopts a wax negative template freezing casting and an indirect forming mode, retains the design freedom of a complex structure, solves problems such as resolution limitation, shrinkage deformation and support difficulty that are faced by direct 3D printing of porous ceramic, and has good use effect.

The invention discloses a model construction method of a gradient random porous structure of a simulated bone trabecula, a white calendite piezoelectric bone scaffold and a preparation method of the white calendite piezoelectric bone scaffold, and the model construction method provided by the invention is a design method based on a Thiessen polygon porous structure with seed point release gradient change and volume scaling coefficient linear gradient change. In the body center scaling process, interpolation calculation is conducted on scaling coefficients from the axis to the outermost end in the cylindrical domain in the radial direction, and therefore the model of the stochastic gradient porous structure with the pore diameter decreasing from inside to outside in the radial direction and the pore edge diameter increasing from inside to outside in the radial direction is finally formed, by designing the formula and process parameters of the white calcium stone ceramic slurry, the possible problems of over-curing, cracking and the like are avoided, and the white calcium stone piezoelectric ceramic bone scaffold with excellent bonding performance is prepared by adopting a 3D printing photocuring printing technology.

The invention discloses a collagen and hydroxyapatite composite bone scaffold as well as a preparation method and application thereof, and relates to the technical field of bone scaffolds. The preparation method of the collagen and hydroxyapatite composite bone scaffold comprises the following steps: dissolving collagen in an acid solution to obtain a collagen solution with the concentration of 10-80 mg / L, mixing the collagen solution with hydroxyapatite, and stirring to obtain a compound; freeze-drying the compound, and cross-linking to obtain a primary product; washing the primary product with water to remove acid residues, and then freeze-drying to obtain the collagen and hydroxyapatite composite bone scaffold. The composite bone scaffold is spongy, has relatively high porosity, is beneficial to bone conduction and bone induction, is not easy to disintegrate and slag when being wetted, and is relatively good in supporting property.

The invention belongs to the technical field of bionic bone scaffolds, and particularly relates to a TPMS structure bionic bone scaffold with an immune regulation function and a preparation method of the TPMS structure bionic bone scaffold. Comprising the following steps: preparing a three-period extremely-small curved surface bionic scaffold and micro-tissues, loading micro-tissue spheres in a methacryloyl gelatin solution, then pouring the micro-tissue spheres into the three-period extremely-small curved surface bionic scaffold, and carrying out photo-crosslinking gel forming to obtain the three-period extremely-small curved surface structure bionic bone scaffold with the immune regulation and control function. The bionic bone scaffold disclosed by the invention can be used for accurately simulating components and structures of natural bones, has good immunoregulation and osteogenesis promoting capabilities, and is expected to cooperate with functionalized micro-tissues to construct an immune microenvironment and promote bone tissue regeneration.

The invention discloses a porous diamond reinforced polylactic acid composite bone scaffold and a preparation method thereof, and belongs to the technical field of biomedical implants. The stent is integrally formed through a selective laser sintering technology and is composed of a polylactic acid matrix and porous diamonds uniformly dispersed in the polylactic acid matrix, and the mass fraction of the porous diamonds is 2%-16%. The surface of the porous diamond is rich in porous structures and oxygen-containing functional groups, the porous diamond and a polylactic acid matrix form a molecular bonding and mechanical interlocking synergistic enhancement interface, and the mechanical property of the polylactic acid composite bone scaffold is remarkably improved. The preparation method comprises the following steps: (1) weighing polylactic acid powder and porous diamond powder according to a set proportion, and uniformly mixing through vacuum ball milling to obtain composite powder; and (2) performing selective laser sintering on the composite powder in an argon protective atmosphere, and integrally forming to obtain the composite bone scaffold. The problem that traditional diamond particle reinforced phase interface bonding is weak is solved, and the method has wide application prospects in the field of bone tissue engineering.

Disclosed is a use of G007-LK in promoting osteogenic differentiation of dental mesenchymal stem cells and bone tissue regeneration. In vitro experiments of the present application show that G007-LK has the ability to induce SHED to form mineralized nodules and promote osteogenic differentiation; in vivo experiments show that G007-LK pretreatment of SHED for 7 days combined with Geistlich Bio-Oss® collagen bone scaffold can enhance the in vivo osteogenic effect of SHED and promote the subcutaneous ectopic osteogenesis of nude mice, indicating that G007-LK has good osteoinductivity and is a potential osteogenic drug. Therefore, G007-LK can promote osteogenic differentiation of dental mesenchymal stem cells and be applied to bone tissue regeneration.

The utility model relates to the technical field of medical instruments, in particular to a drug sustained-release cartilage support. The drug sustained-release cartilage stent comprises a plurality of support units which are overlapped in sequence, each supporting unit comprises two polygonal structures, and the two polygonal structures are connected in a three-dimensional mode through a plurality of parallel V-shaped connecting parts. And the polygonal structure is formed by connecting two groups of parallel V-shaped connecting parts end to end. The drug sustained-release cartilage stent can be compressed when being implanted, and rebounds after being implanted, so that mechanical support is realized. Meanwhile, triangular bulges or grooves are formed in the surface of the porous scaffold constructed by the V-shaped connecting parts, so that embedding with surrounding tissues is facilitated.

The invention relates to a bionic bone scaffold customized body and a preparation method and application thereof, and belongs to the technical field of biomedical materials. The invention provides a preparation method of a bionic bone scaffold customized body, which comprises the following steps: scanning and recording a cranio-maxillofacial hard tissue defect form, and constructing a digital scaffold model through an algorithm model and / or artificial design based on the recorded data; performing three-dimensional reconstruction on the obtained digital scaffold model to generate bionic bone scaffold customized volume data meeting the personalized requirements of cranio-maxillofacial tissue defects; and converting the obtained customized body data of the bionic bone scaffold into a customized body of the bionic bone scaffold by utilizing a 3D printing technology. Modeling is conducted through real bone defect data, a bionic bone scaffold customized body of the bone defect part is prepared through the 3D printing technology, the outer contour is attached to the bone defect side form, the ideal bone form is reconstructed, the inner contour is a fine substructure of the defect part, and the bionic bone scaffold has a bionic pore structure and biomechanical performance; the method can be used for reconstructing personalized cranio-maxillofacial tissues.

This application relates to the technical field of cancellous bone scaffolds, and discloses a method and apparatus for determining a reverse generation model of cancellous bone scaffolds based on topology optimization. In this method, the three-dimensional thin-layer structure is topology optimized to mimic the complex and irregular microstructural features of cancellous bone tissue. The first and second multi-channel mechanical contour maps represent force-related features. Multimodal data consisting of the three-dimensional thin-layer structure, the initial simplified model, the first multi-channel mechanical contour map, and the second multi-channel mechanical contour map are used as training samples to train a latent graph diffusion model. This allows the model to achieve powerful output performance for cancellous bone scaffolds under multimodal input conditions.

The present application belongs to the technical field of biological scaffolds, and particularly relates to a preparation method of a porous biomimetic bone scaffold and the biomimetic bone scaffold. The preparation method of the porous biomimetic bone scaffold comprises the following steps: mixing a hydrogel main material, water, a crosslinking agent, an alkyl amine compound and an optional complex high polymer material; and performing directional freezing treatment on the obtained mixed product, wherein the hydrogel main material is selected from one or more than two of silk fibroin, collagen and gelatin, and the complex high polymer material is selected from one or more than two of hyaluronic acid, chitosan and other biological source natural polymer and synthetic organic high polymer compound. The porous biomimetic bone scaffold with certain pore size directional micropore channels and nanochannels formed along the side walls of the directional micropore channels can be prepared by the method.

The invention discloses a lateral flow fishbone-shaped inclined plate assembly and an inclined plate sedimentation tank. The inclined plate assembly comprises a fishbone bracket, an inclined plate connecting piece and an inclined plate, the inclined plate is flat-plate-shaped; the mounting part of the inclined plate connecting piece is in a long thin plate shape and is obliquely arranged; the upper end of the inclined plate is mounted on the mounting part; an extension part is arranged on the lower side of the mounting part; a flushing assembly for flushing the inclined plate is mounted at the lower end of the extension part; the fishbone brackets are vertically arranged; a plurality of pairs of inclined plate connecting pieces are arranged in the length direction of the fishbone support, each pair of inclined plate connecting pieces are oppositely arranged on the two sides of the fishbone support and connected with the fishbone support through the connecting parts of the inclined plate connecting pieces, and the lower ends of the mounting parts incline in the direction away from the fishbone support. According to the invention, the unique inclined plate structure and the fishbone-shaped inclined plate assembly are adopted, and the flushing assembly is arranged to effectively flush the inclined plate, so that the permissible length of the inclined plate is effectively increased, the maintenance interval is reduced, and the precipitation effect is improved while the structural stability of the inclined plate assembly and the inclined plate module is ensured.

The application discloses a design method of a bionic bone support based on TPMS and belongs to the technical field of bionic bone supports. The application is based on G unit and D unit formulas, adjusts a bias function to generate G gradient unit and D homogeneous unit CAD models; uses a fusion function to fuse the G gradient unit and the D homogeneous unit CAD models to generate a GD radial fusion CAD model, adjusts the bias function of the G gradient unit by using an interpolation method to make the GD radial fusion CAD model reach a target average porosity; reduces the wall thickness of the GD radial fusion CAD model to form a GD radial fusion CAD model with wall thickness; then divides the GD radial fusion CAD model with wall thickness into a finite element simulation grid model and outputs an inp format simulation model file; imports the inp format simulation model file into HyperMesh software, repairs and divides the grid and exports an inp file format model. The porous support designed by the application considers different functional grading of natural bone structures and different cell morphological fusion, better meets the mechanical properties of a natural femur, and light weight design increases the specific surface area to provide more space for bone cell adhesion and growth.

According to the multi-component synergistic 3D printing multi-structure long-acting anti-infection bone repair composite scaffold and the preparation method thereof, the method comprises the following steps: preparing a PVA aqueous solution as a slurry adhesive, and mixing HA, beta-TCP, the PVA aqueous solution and glycerol as a printing slurry; 3D printing parameters are set, a model is imported, a printing structure, discharging and wiring speeds are set, and a customized support is obtained through 3D printing; drying and sintering the scaffold to obtain a porous bone scaffold without organic matters; preparing porous microspheres ABX coated PLGA loaded with antibiotics by taking gelatin as a pore-foaming agent; and coating the sintered porous bone scaffold with a gelatin coating, and loading the microspheres on the porous bone scaffold to obtain the drug-loaded composite bone scaffold. The bone repair scaffold disclosed by the invention is combined with an antibiotic local drug delivery system to prevent infectious bone defects, can overcome the defects of systemic drug delivery of antibiotics, realizes controllable release of drugs in the whole process in a bone regeneration period, has good antibacterial performance while repairing bone defects in vivo, and also has relatively good compressive strength and biocompatibility.

This invention discloses a method for preparing a composite nanofiber bone scaffold, belonging to the field of medical engineering technology. First, a bacterial cellulose (BC) membrane is prepared. BC membrane powder is then prepared by pre-cooling with liquid nitrogen and mechanical grinding, and subsequently dispersed in a polydopamine solution to obtain a BC polydopamine solution. The prepared decellularized submucosal layer (SIS) of the biological matrix membrane is incubated overnight in the BC polydopamine solution, and finally freeze-dried to obtain the composite nanofiber bone scaffold. This invention provides a method for preparing a composite bone scaffold using polydopamine, which has excellent film-forming properties and biocompatibility, does not hinder cell growth and differentiation, and combines BC and SIS in a green synthesis process with simple operation.

This invention belongs to the field of medical materials technology, specifically referring to a drug-loaded porous magnesium alloy bone scaffold and its preparation method. The drug-loaded porous magnesium alloy bone scaffold is composed of the following raw materials in parts by weight: 55 parts magnesium alloy, 6-10 parts corrosion inhibitor, 1 part drug-loaded gel, 2-4 parts antibacterial bone-promoting agent, and 3 parts gelatin. This invention achieves sustained drug release by fabricating magnesium alloy into microtubes and filling the interior with a drug-loaded hydrogel; gelatin is sprayed onto the surface and adhered through Mg-N coordination bonds, bonded to the corrosion inhibitor, and hot-pressed. The components form an interpenetrating network with the gelatin to enhance bonding and react with the magnesium alloy to form a protective film; simultaneously, Ca... 2+ The process initiates cross-linking of sodium alginate, forming a stable anti-corrosion barrier. Finally, an antibacterial and bone-promoting mixture containing modified chitosan and thermosensitive polymer is sprayed on. Through complexation reaction and temperature control, the bone-promoting components are evenly dispersed around the implant, improving tissue compatibility.

The application discloses a perspective image analysis method for bionic repair of bone tumors, comprising the following steps: acquiring perspective image data of a bone tumor area; performing cross-modal feature alignment on the data, constructing a three-dimensional bone tumor image model in a three-dimensional voxel space coordinate system, and eliminating image artifacts; performing segmentation processing on the bone tumor through a deep learning segmentation network, identifying a bone tumor infiltration boundary, and acquiring three-dimensional morphological parameters and bone tumor characteristic parameters of the bone tumor area; reconstructing a bionic scaffold structure of a bone defect area through biomechanical modeling, and implanting the bionic scaffold into the bone tumor area; and acquiring bone-scaffold interface fusion rate, stress distribution uniformity and new bone growth depth indexes based on postoperative image data. The application has the advantages that: through the combination of accurate segmentation at the voxel level and a convolutional neural network, the bone tumor area and morphology can be accurately identified and divided, thereby providing reliable support for subsequent tumor infiltration evaluation and bionic scaffold design.

This invention discloses a highly active ceramic-collagen composite bone scaffold and its preparation method, belonging to the field of bone repair material preparation technology. Quaternized ammonium inorganic material inclusions, photosensitive resin, and dispersant are mixed and ball-milled to obtain a mixed slurry. The mixed slurry is then printed to obtain a bioceramic porous scaffold green body. The bioceramic porous scaffold green body is sintered to obtain a bioceramic porous scaffold. The bioceramic porous scaffold is then vacuum-mixed with collagen fiber slurry, followed by post-treatment to obtain a ceramic-collagen composite scaffold. The ceramic-collagen composite scaffold is then impregnated with rhBMP-2 solution to obtain the highly active ceramic-collagen composite bone scaffold. The preparation method of this invention not only solves the mechanical properties of the prepared bone scaffold but also achieves good osteogenic effects.

The invention relates to the technical field of minimum-curved-surface bone scaffold wall thickness optimization, in particular to a minimum-curved-surface bone scaffold wall thickness optimization method based on stress guidance. The method comprises the following steps: constructing a Gyandroid support model with a Sheet configuration on the basis of a Gyandroid implicit equation in a three-period minimal curved surface, and constructing a Diamond support model with the Sheet configuration on the basis of a Diamond implicit equation in the three-period minimal curved surface; performing mechanical compression simulation on the Gyandroid support model to determine a high stress area of the Gyandroid support model, and performing mechanical compression simulation on the Diamond support model to determine a high stress area of the Diamond support model; and determining a stress rule based on the high-stress area of the Gyandroid support model, and marking the high-stress area in the Gyandroid support model through the stress rule to obtain a high-stress marked area of the Gyandroid support model. In this way, the problem of contradiction between compressive strength and biomechanical matching performance can be effectively solved, and excellent biological performance is achieved.

The application provides a ROS response 3D printing shape memory material loaded with camel milk derived exosomes and a preparation method and application thereof, and belongs to the technical field of bone repair materials. The application utilizes 3D printing technology to prepare a porous channel bone scaffold material ISO-PUs-SMP from isosorbide long-chain hard segment polyurethane ISO-PUs, uniformly blends drug-loaded nanofiber microcrystalline beta-C-C-CM-Exo containing camel milk derived exosomes and a gelatin solution, and fills the channel of the ISO-PUs-SMP, so as to prepare the ROS response 3D printing shape memory material through freeze drying. The ROS response 3D printing shape memory material provided by the application has the effects of promoting blood vessels, resisting oxidation and resisting inflammation, can promote angiogenesis at a large bone damage site, reduce the oxidative stress reaction caused by hypoxia, reduce the inflammatory reaction and hypoxic damage, and finally promote the repair and reconstruction of bone tissue at the large bone defect site.

The present invention relates to the field of bone replacement materials and bone scaffolds. In particular, the invention relates to a bone replacement material comprising:-at least one porous calcium silicate-coated particle comprising: a core which is a porous particle having microporosity and macroporosity; and made from at least one calcium phosphate-based compound; and a calcium silicate coating completely or partially embedding the core; the calcium silicate coating comprises 60% to 100% by weight of at least one calcium silicate compound based on the total weight of the calcium silicate coating; and optionally, at least one uncoated porous particle having microporosity and macroporosity and made of at least one calcium phosphate-based compound. The invention also relates to a bone scaffold resulting from hydration of a bone substitute material of the invention; and a method for manufacturing the bone replacement material.

The invention discloses a bionic silk fibroin cartilage scaffold for skull defect repair, and belongs to the technical field of biomedical engineering. In order to solve the problems that an existing skull repair stent is single in structure, low in osteogenesis efficiency and unmatched in mechanical property and degradation rate, the invention provides a stent which sequentially comprises a bionic periosteum layer, a bone conduction and vascularization layer and a cartilage induction layer from outside to inside. Wherein the bionic periosteum layer is of a compact nanofiber membrane structure and plays a role in physical barrier and osteogenesis induction; the bone conduction and vascularization layer is of a gradient porous structure with the pore diameter gradually reduced from outside to inside and aims at guiding blood vessels and cells to grow in orderly and promoting rapid vascularization. The cartilage induction layer is of a spongy microporous structure and is used for forming a stable cartilage template and starting osteogenesis in cartilage. By means of structural bionic and functional partition, the natural bone healing process is simulated, vascularization and osteogenesis are promoted in a synergistic mode, and efficient biological repair of skull defects is achieved.

The invention discloses a bionic bone scaffold design method based on an ellipsoid random intersection distribution algorithm, and relates to the field of bionic bone scaffold design, and the method comprises the following steps: S1, obtaining a CT image of a target skeleton cross section based on Micro-CT to generate a porous structure of cancellous bone; s2, analyzing the microporous structure of the cancellous bone to determine the size of a fitting ellipse; s3, the center distance of the fitting ellipse is set based on the size of the fitting ellipse, and an ellipse center dot matrix is generated; s4, rotating the fitting ellipse based on the long axis of the fitting ellipse to generate an ellipsoid unit, and generating a random rotation angle of the current ellipsoid unit through a random function; s5, repeating the copying, shifting and rotating instructions of the ellipsoid units in combination with the loop statement and the ellipsoid center dot matrix to obtain a cylinder model composed of the ellipsoid units, and generating the bionic bone scaffold based on the cylinder model. The bionic bone scaffold beneficial to growth and proliferation of cells and transmission of nutrient substances is obtained through ellipse fitting.

The invention provides composite bio-ink, a 3D printing bone scaffold and a preparation method and application of the 3D printing bone scaffold, and belongs to the technical field of biomedical materials and bone tissue engineering. The composite biological ink provided by the invention is prepared from the following raw material components: 1 percent to 3 percent (w / v) of methacrylic acid konjac glucomannan (KGM-MA), 1 percent to 3 percent (w / v) of nano-hydroxyapatite (nHAp), 0.25 percent to 1.0 percent (w / v) of a photoinitiator and 0.001 percent to 0.01 percent (w / v) of Cu2O and d-RuO2 nano-enzyme, the composite bio-ink is used for preparing an osteogenic scaffold through 3D printing. The Cu2O d-RuO2 nano-enzyme and the n-HAp / KGM-MA hydrogel matrix are combined through the 3D printing technology, structure customization and function collaboration are achieved, the 3D printing bone scaffold can continuously release active components in the degradation process, all the components cooperate to promote angiogenesis and bone tissue regeneration, and the bone scaffold has the advantages of being capable of achieving the purpose of improving the bone tissue regeneration rate and the bone tissue regeneration rate. The material shows good biocompatibility, osteogenesis inductivity and microenvironment adjusting ability in bone defect repair, and is suitable for application in the field of bone injury repair.

The application provides a beta-TCP / PLGA support sustained-release body loaded with Cu-based nanoscale enzyme and a preparation method, a 3D model of a microstructure of a bone defect is established by measuring bone structure parameters, and a personalized beta-TCP / PLGA support is printed by using beta-TCP / PLGA printing materials through a 3D bioprinter technology. The beta-TCP / PLGA support is immersed in a Cu-HCF nanoscale enzyme solution, so that the beta-TCP / PLGA support is loaded with Cu-HCF nanoscale enzyme, thereby preparing a Cu-HCF nanoscale enzyme beta-TCP / PLGA sustained-release support. In a tumor and inflammatory microenvironment, the enzyme reaction can selectively kill tumor and inflammatory cells, and can protect normal tissues, reduce the possibility of recurrence of bone tumors and inflammation at the bone defect, and avoid possible side effects of drugs on the human body. As a kind of bionic bone support, the beta-TCP / PLGA sustained-release support has good mechanical strength and biodegradability, and also has the effect of keeping a long-acting and stable drug release, can improve the directionality and reduce the toxic side effects. The material cost of the beta-TCP / PLGA sustained-release support is moderate and relatively easy to prepare, and has good mass production prospects and economic applicability.

Disclosed is a use of G007-LK in promoting osteogenic differentiation of dental mesenchymal stem cells and bone tissue regeneration. In vitro experiments of the present application show that G007-LK has the ability to induce SHED to form mineralized nodules and promote osteogenic differentiation; in vivo experiments show that G007-LK pretreatment of SHED for 7 days combined with Geistlich Bio-Oss® collagen bone scaffold can enhance the in vivo osteogenic effect of SHED and promote the subcutaneous ectopic osteogenesis of nude mice, indicating that G007-LK has good osteoinductivity and is a potential osteogenic drug. Therefore, G007-LK can promote osteogenic differentiation of dental mesenchymal stem cells and be applied to bone tissue regeneration.

The application discloses a multifunctional composite coating for a zinc-based implant, which is prepared on the surface of the zinc-based implant, and is prepared by using poly-tannic acid as a bottom layer and grafting epsilon-polylysine on the bottom layer through a covalent bond; the application also discloses a preparation method of the composite coating, which comprises the following steps: preparing a poly-tannic acid bottom layer on the surface of the zinc implant, and then grafting epsilon-polylysine to obtain a zinc-based implant with the multifunctional composite coating, and the zinc-based implant is applied to the field of implants related to biological and pharmaceutical materials. The multifunctional composite coating can induce platelet adhesion and activation, trigger a blood coagulation cascade reaction, and quickly form a stable blood coagulation layer to provide an ideal microenvironment for bone repair; meanwhile, the introduction of epsilon-polylysine enables the coating to have a strong and long-lasting contact antibacterial capacity, and the two can synergistically promote early osteogenesis; the preparation process is simple, the conditions are mild, the raw materials have good biocompatibility, and the multifunctional composite coating is suitable for surface functionalization modification of various implant instruments such as zinc-based bone nails, bone plates and bone scaffolds.