130 results about "Bone scaffold" patented technology

The invention provides an interface-enhanced regenerated bone scaffold for sustained-release medicines as well as a preparation method and application of the interface-enhanced regenerated bone scaffold, and belongs to the technical field of bone tissue engineering. According to the invention, polycaprolactone and hydroxyapatite are taken as matrix components of the regenerated bone scaffold, so that the regenerated bone scaffold has mechanical strength matched with a host bone and good biocompatibility; the polydopamine coating is loaded on the surface and internal pores of the regenerated bone scaffold matrix, surface functional modification is easy to perform, and a catechol structure in the polydopamine coating can perform coordination binding with metal ions in MOFs, so that the falling rate of MOFs particles is reduced, and the MOFs particles are uniformly and stably distributed; the MOFs particles are used for loading the eucommia ulmoides extract, safety and non-toxicity are achieved, the eucommia ulmoides extract is encapsulated in the MOFs particles, the stability of the eucommia ulmoides extract can be improved, and a better drug release effect and a better bone regeneration promoting effect are achieved.

The invention provides a design method of a personalized gradient porous bone scaffold based on patient apparent bone mineral density. The design method comprises the following steps: constructing a variable density gradient porous structure of the bone scaffold; obtaining sample data through compression mechanical simulation and fluid analogue simulation; constructing a BP neural network model; the apparent bone mineral density of a patient is obtained through detection, the theoretical elastic modulus is calculated, and the mechanical-permeability coordinated gradient bone scaffold structure parameters are predicted through a BP neural network model; and an intelligent platform for designing a personalized gradient porous bone scaffold structure is built. The method has the beneficial effects that the gradient porous structure is constructed by controlling the offset in the TPMS function, and the bone scaffold gradient porous structure which is matched with the apparent bone density of a patient and is coordinated in mechanical and penetrating performance is realized through performance simulation and neural network data processing; and a bone scaffold parameter prediction platform is established to provide a personalized bone scaffold structure design scheme for convenient and efficient bone repair medical treatment.

The invention discloses a bone scaffold fusion design and preparation method based on mirror image registration, and the method comprises the steps: firstly, based on a three-dimensional medical image of a femoral defect region of a patient, combining an opposite-side complete femoral CT image, and achieving the automatic recognition and complementation of the defect region through the mirror image transformation and ICP registration technology; after defect boundary extraction is completed, three-dimensional reconstruction is carried out on the individualized outer contour of the left bone tissue, and an STL format model is exported. According to the femur functional partition characteristics, a Diamond structure is adopted in a cortical bone area to enhance mechanical support, and a Gyandroid structure is selected in a cancellous bone area to achieve porosity gradient filling, so that the permeability is optimized, and cell proliferation is promoted. Continuous transition among the structures is realized through a Sigmoid function, and the stability and the biological adaptability of the whole structure are improved. And finally, the stent model is subjected to selective laser melting, and a Zn-3Mg alloy material is utilized to complete printing manufacturing.

The invention discloses a 3D printing self-adaptive control system for bone defect, and relates to the technical field of 3D printing self-adaptive control, a miniaturized optical coherence tomography, a high-resolution camera, a force sense and temperature sensor and the like are installed on an operation site, and the whole process of material deposition and solidification is monitored; then, deviation is rapidly recognized through an anomaly detection and error prediction model, defect positions are output, and a closed-loop control unit is combined with model prediction control and self-adaptive path planning to achieve online adjustment of key variables such as the extrusion amount and the printing speed; when the defect cannot be made up automatically through parameter adjustment, the system can trigger local shutdown and secondary repair, the forming quality is evaluated through multi-modal scanning and a repair scoring function after repair, and the high matching degree and mechanical stability of the final bone scaffold and the bone defect part are ensured; the cooperation of multi-modal sensing and local path planning enables the system to have higher adaptability.

The invention discloses a method for designing a bionic bone scaffold based on wall thickness mixed gradient change, and belongs to the technical field of bionic bone scaffolds. By designing the wall thickness mixed gradient function, the lattice unit can have wall thickness gradient change in the radial direction and the axial direction at the same time, so that the porosity also has gradient change in three directions at the same time, and the defect that a bone scaffold is designed by using a lattice material (beam unit) unit and a TPMS unit in the prior art can be well overcome; and the stent better conforms to the natural bone structure and mechanical property requirements. The designed bionic bone scaffold is excellent in mechanical property, high in compression resistance and deformation resistance and capable of promoting cell growth and tissue regeneration.

The invention discloses a light-driven / temperature-driven double-trigger shape memory antibacterial and anti-tumor bone scaffold and a fused deposition modeling (FDM) preparation method thereof, and is characterized in that polylactic acid (PLA) is used as a matrix, and polyethylene glycol (PEG) and nano magnesium hydroxide (Mg (OH) 2) / copper oxide (CuO) are uniformly dispersed through an organic solvent blending method; a composite wire is prepared through the processes of drying and film forming, crushing and granulation and screw extrusion, and finally, the integrated molding of the three-dimensional porous scaffold is realized through an FDM (fused deposition modeling) process. According to the stent, temperature and near-infrared light double-triggered shape memory is achieved through cooperation of glass transition regulation and control of PLA-PEG and the photothermal effect of the nano metal oxide particles. Nano Mg (OH) 2 / CuO endows the scaffold with multi-mode antibacterial and anti-tumor functions: 1) Mg < 2 + > / Cu < 2 + > releases and destroys microbial membrane potential; according to the stent, the limitation of shape fixation of a traditional implant is broken through, shape self-adaption can be triggered through light / heat stimulation, irregular bone defects can be accurately attached, the risk of a secondary operation is avoided, and the stent has remarkable advantages in minimally invasive orthopedic repair.

The invention discloses a design method of a bionic bone scaffold based on a TPMS (Tire Pressure Monitor System), and belongs to the technical field of bionic bone scaffolds. According to the method, on the basis of a G unit formula and a D unit formula, a bias function is adjusted to generate a G gradient unit CAD model and a D homogeneous unit CAD model; fusing the CAD model of the G gradient unit and the CAD model of the D homogeneous unit by using a fusion function to generate a GD radial fusion CAD model, and adjusting an offset function of the G gradient unit by adopting an interpolation method to enable the GD radial fusion CAD model to reach a target average porosity; reducing the wall thickness of the GD radial fusion CAD model to form a GD radial fusion CAD model with the wall thickness; then, a finite element simulation grid model is divided, and an inp-format simulation model file is output; importing the simulation model file in the inp format into HyperMesh software for repairing, and dividing grids to export the model in the inp file format. According to the porous scaffold designed by the invention, different functional grades of a natural bone structure are considered, and different unit cell polymorphic fusion is carried out, so that the porous scaffold better conforms to the mechanical properties of natural thighbones, and the specific surface area is increased by virtue of the lightweight design, so that more space is provided for bone cell adhesion and growth.

The invention provides a 3D printing bone scaffold based on a TPMS structure, the bone scaffold is prepared by taking Ti-6Al-4V powder as a building raw material through a 3D printing mode, the bone scaffold is a TPMS G unit structure, the aperture range is 420-630 microns, the inner layer porosity is 55%, the peripheral porosity is 90%, the diameter of the scaffold is 15 mm, and the height of the scaffold is 2 mm. Moxifloxacin / rifampicin PLGA microspheres and BMP-2 are loaded in the bone scaffold and are fixed by hydrogel, so that the 3D printing anti-infection bone scaffold based on the TPMS structure is formed. The prepared 3D printing anti-infection bone scaffold highlights excellent performance in all aspects, has good capacity of resisting infection and promoting bone formation, and provides a new material and thought for treatment of infectious bone defects. In addition, the invention further discloses a preparation method and application of the stent.

A bone scaffold structure may include one or more scaffold units, wherein each of the scaffold units comprises a first end profile, a second end profile, and one or more wires connecting the first end profile to the second end profile. Each of the first end profile and the second end profile may be defined by a polygon surface that is specified by a center and a number of vertices that are pairwise connected by a side. Each of the one or more wires may be specified by a wire length and a substantially circular cross-section with a first end of each of the one or more wires being connected to a vertex of the polygon surface defining the first end profile and a second end of each of the wires being connected to a vertex of the polygon surface defining the second end profile.

The invention discloses a bone immunoregulation osteogenesis promoting method of PLL-MgBGs / SF frozen gel and an osteogenesis capability detection method of the PLL-MgBGs / SF frozen gel. The bone immunoregulation osteogenesis promoting method comprises the following steps: preparing a magnesium-doped bioactive substance MgBGs; the preparation method comprises the following steps: preparing PLL-MgBGs nano particles; pLL-MgBGs / SF frozen gel is prepared; a sterile PLL-MgBGs / SF frozen gel leaching solution is prepared; the method comprises the following steps: inoculating macrophages (RAW264.7) into a 48-pore plate, culturing by using a complete culture medium containing LPS (100 ng / mL), and then culturing by using a complete culture medium containing a PLL-MgBGs / SF frozen gel leaching solution. The PLL-MgBGs / SF freezing gel has the advantages that the prepared PLL-MgBGs / SF freezing gel is excellent in water response shape memory ability, capable of releasing various active ions such as Mg < 2 + >, Ca < 2 + > and SiO4 < 4->, remarkable in potential in immunoregulation and bone repair, capable of being implanted into bone defects in an injection mode to fill the bone defects, capable of being rapidly matched with irregular bone defects and capable of improving the bone repair effect. According to the present invention, the problem of insufficient matching of the traditional bone scaffold and the irregular defect site is solved, the excellent mechanical stability and the excellent structure retention can be represented during the degradation process, the good supporting ability is provided in the bone defect region, the polarization of the macrophage can be adjusted to be the M2 type, and the collection, the proliferation and the osteogenic differentiation of the bone marrow mesenchymal stem cells (BMSCs) can be promoted.

The invention discloses a preparation method of a cobalt ferrite manganese ferrite L-polylactic acid composite antibacterial bone scaffold, which comprises the following steps: growing manganese ferrite on the surface of cobalt ferrite in situ to prepare cobalt ferrite-manganese ferrite composite powder, and adding the prepared cobalt ferrite-manganese ferrite composite powder into ethanol to prepare a suspension; the preparation method comprises the following steps: preparing cobalt ferrite-manganese ferrite and poly-L-lactic acid composite powder, performing ultrasonic mixing with poly-L-lactic acid ethanol suspension, performing centrifugal drying to obtain the cobalt ferrite-manganese ferrite and poly-L-lactic acid composite powder, and finally performing selective laser sintering to obtain the cobalt ferrite-manganese ferrite / poly-L-lactic acid composite antibacterial bone scaffold. As a novel bone implant, the cobalt ferrite-manganese ferrite / poly-L-lactic acid composite antibacterial bone scaffold prepared by the invention can generate local high temperature and a large amount of active oxygen under the excitation of an alternating magnetic field, so that a good antibacterial effect is achieved.

The invention discloses a method for designing a bionic bone scaffold based on TPMS unit and lattice unit multi-plane fusion, and belongs to the technical field of bionic bone scaffolds. The porosity is adjusted by adjusting the wall thickness, so that an S and SC homogeneous unit porous structure with the target porosity is formed, and then a Schwarz unit cell and a simple cubic unit cell are subjected to plane smooth transition fusion in a fusion region or fusion function tan (x / w) mode; and respectively importing the established fusion model into mechanical simulation software to carry out quasi-static compression finite element simulation so as to obtain the elastic modulus and the yield strength which accord with the thighbone. According to the design, different types of TPMS units and traditional lattice units are subjected to heterogeneous unit fusion, the fused novel porous structure model has different function grading expressions, and a new thought is provided for design of the porous structure as a bionic bone scaffold.

The invention discloses a shape memory alloy bone scaffold imitating a Haversian system as well as a 4D printing preparation method and application of the shape memory alloy bone scaffold. According to the 4D printing preparation method, the stress state and function requirements of the bone scaffold are fully considered, a bone scaffold porous structure with an imitated Haversian system is designed by adopting a function bionic method, and the bone scaffold is prepared by adopting a 4D printing technology. The inner layer imitated cancellous bone porous structure designed in the preparation method not only meets the requirement that the inner layer has good elasticity and toughness, but also facilitates cell adhesion to guide cell growth and maintains blood supply; the designed artificial cortical bone porous structure with the Haversian system on the outer layer meets the requirement that the outer layer has good compression resistance and bending resistance, and the Haversian system is more beneficial to ingrowth of blood vessels and nerves and maintains skeleton metabolism and repair. The shape memory alloy bone scaffold simulating the Haversian system, disclosed by the invention, has a wide application prospect in the field of bone implants.

The invention relates to the technical field of bone tissue engineering and regenerative medicine, and discloses a stem cell-loaded bionic bone scaffold material, which comprises a bone scaffold main body, the bone scaffold main body is formed by compounding hydroxyapatite and a biodegradable high-molecular polymer and has a porous structure, the porosity is 70%-90%, the aperture range is 100-500 microns, and pores are communicated with one another; a vascularization induction factor slow release system is arranged in the porous structure of the bone scaffold main body, the stem cell-loaded bionic bone scaffold material does not need to take the autologous bone of a patient, and the problems of donor injury and limited sources are avoided; the composite scaffold is matched with low-immunogenicity stem cells, so that the risks of immunological rejection and disease transmission are avoided; the material has the advantages of good biocompatibility, mechanical matching with human bones, degradability, no secondary operation, promotion of vascular regeneration and stem cell osteogenesis, and realization of bone regeneration integration.

According to the method for designing the biological bone scaffold of the tetragonal symmetric lattice structure based on the minimal curved surface, reconstruction is performed based on a classic three-period minimal curved surface lattice structure implicit function for the biological bone scaffold, and a novel implicit function of the three-period minimal curved surface lattice structure is obtained; and judging whether a lattice structure obtained on the basis of the reconstructed implicit function meets a set constraint condition or not, and if so, designing and preparing the biological bone scaffold by using the reconstructed implicit function. Therefore, the biological bone scaffold prepared from the tetragonal symmetrical lattice structure has good mechanical properties and material transport performance on the premise of light weight.

The invention aims to provide a polycitrate composite porous bone scaffold and a preparation method, and relates to the technical field of medical instruments, citric acid and 1, 8 octylene glycol are subjected to thermal polymerization crosslinking at high temperature to prepare polycitrate, PCL is added, esterification reaction is performed to synthesize polycaprolactone-citrate (POCL), and the polycaprolactone-citrate (POCL) composite porous bone scaffold is prepared. Then respectively grafting ALN and RSV-loaded calcium carbonate microspheres prepared by a calcium chloride dihydrate and ammonium bicarbonate coprecipitation method on the POCL support by adopting a covalent binding mode; the calcium carbonate microspheres loaded with the RSV are successfully prepared, the microspheres can be degraded in an acid environment and release the RSV, the novel poly-citrate porous bone scaffold loaded with the ALN and the RSV microspheres is successfully constructed, the scaffold has good mechanical properties, hydrophilicity and stability, the acid environment of degradation products of the poly-citrate scaffold can be effectively improved, and the stability of the poly-citrate porous bone scaffold is improved. Meanwhile, the scaffold has good biocompatibility, can promote proliferation of the BMSCs, and has an osteogenic induction effect, so that bone repair is promoted.

The application discloses a lateral flow fishbone-shaped inclined plate assembly and an inclined plate sedimentation tank. The inclined plate assembly comprises a fishbone support, an inclined plate connecting piece and an inclined plate. The inclined plate is in the form of a flat plate. The mounting portion of the inclined plate connecting piece is in the form of a long and thin plate and is arranged in an inclined manner. The upper end of the inclined plate is mounted on the mounting portion. An extension portion is arranged on the lower side of the mounting portion. A washing assembly for washing the inclined plate is mounted on the lower end of the extension portion. The fishbone support is arranged in a vertical arrangement. A plurality of pairs of inclined plate connecting pieces are arranged along the length direction of the fishbone support. Each pair of inclined plate connecting pieces is oppositely arranged on the two sides of the fishbone support. Each pair of inclined plate connecting pieces is connected with the fishbone support through the connecting portion thereof. The lower end of the mounting portion is inclined towards the direction away from the fishbone support. The application adopts a unique inclined plate structure and a fishbone-shaped inclined plate assembly, and is provided with a washing assembly for effectively washing the inclined plate. The application effectively increases the allowable length of the inclined plate while ensuring the structural stability of the inclined plate assembly and the inclined plate module, reduces the maintenance interval, and improves the sedimentation effect.

The invention relates to the technical field of bone defect repair, in particular to a composite cylindrical bionic bone scaffold and a preparation method thereof.The preparation method comprises the following steps of regenerated material preparation, ZP scaffold preparation, composite hydrogel preparation, PH scaffold preparation and scaffold assembling, specifically, the ZP scaffold and the PH scaffold are subjected to ultraviolet disinfection treatment, then a phosphate buffer solution is used for flushing treatment, and the composite cylindrical bionic bone scaffold is obtained; the preparation method comprises the following steps: implanting endothelial cells into a PH scaffold according to 1 * 10 < 5 > mL, carrying out perfusion culture for 6-8 days, and combining a ZP scaffold with the PH scaffold in which the endothelial cells are implanted to form the macro-microstructure composite cylindrical bionic bone scaffold. According to the composite cylindrical bionic bone scaffold and the preparation method thereof, the ZP scaffold and the PH scaffold are combined to form the vascularized composite cylindrical bionic bone scaffold containing internal and external bionics, so that the composite cylindrical bionic bone scaffold has satisfactory biocompatibility, and the angiogenesis and osteogenesis processes are obviously promoted.

The invention discloses a method for designing a bionic bone scaffold based on unit cell size gradient change, and belongs to the field of biological material design. The method comprises the following steps: calculating the overall wall thickness of FCC, TH and G cell size gradient models according to the required porosity; a unit is selected as a unit cell size radial gradient, in order to better simulate a natural bone structure, the overall porous structure morphology is designed as a cylinder model, and the unit cell sizes of the outer layer and the inner layer of each model are designed as required; the overall porosity of the three novel porous structure scaffolds reaches 55% by changing the wall thickness; carrying out mechanical property testing on the novel porous structure; observing the compression deformation behavior of each model and recording the numerical results of the elastic modulus and the yield strength after simulation; the designed novel porous scaffold has different functional gradients in the radial direction, and the application range of the energy absorption material unit in bionic bone scaffold design is expanded.

The invention relates to the technical field of cancellous bone scaffolds, and discloses a topological optimization-based cancellous bone scaffold reverse generation model determination method and device, in the method, a three-dimensional thin layer structure is subjected to topological optimization processing, and complex and irregular microstructure characteristics of cancellous bone tissues can be simulated. The first multi-channel mechanical nephogram and the second multi-channel mechanical nephogram are force-related features. And multi-modal data composed of the three-dimensional thin-layer structure, the initial simplified model, the first multi-channel mechanical cloud picture and the second multi-channel mechanical cloud picture is used as a training sample to train a potential map diffusion model, so that the model can obtain strong performance of outputting a cancellous bone scaffold result under the training of a multi-modal input condition.

The application provides a alginate-based aerogel, a preparation method and application thereof. The alginate-based aerogel has parallelly arranged large pores and oriented channels at an angle with the large pores, and the angle ranges from 0 to 180 degrees. The large pores are distributed in the same direction inside the alginate-based aerogel, and the oriented channels improve the interconnectivity between the large pores. The existence of the large pores and the oriented channels makes the alginate-based aerogel have the characteristics of anisotropy and a multi-scale pore structure, and the size of the large pores and the oriented channels is suitable for inducing vascularization and osteogenesis. Therefore, the aerogel can be used as a bone scaffold material. Tests show that the aerogel has the characteristics of light weight and high strength. In addition, other components beneficial to osteogenesis can be added in the alginate system, and the size of the pores can be adjusted without affecting the formation of the pore structure.

The invention discloses a bionic bone scaffold composite material prepared through combination of electrostatic spinning and 3D printing and a preparation method of the bionic bone scaffold composite material, and relates to the technical field of bionic bone scaffold composite materials. A bionic bone scaffold composite material prepared by combination of electrostatic spinning and 3D printing is characterized by comprising 80 to 100 parts of a biodegradable high-molecular polymer, 5 to 30 parts of a bioactive inorganic ceramic material, 200 to 500 parts of a solvent, 0.01 to 5 parts of a bioactive factor, 0.1 to 0.5 part of a bacteriostatic active compound and 0.01 to 5 parts of naringin. According to the invention, the metronidazole derivative is used for providing antibacterial ability, and is matched with naringin to realize anti-inflammatory and osteogenesis-promoting synergistic effect, so that the problem of drug resistance or non-uniform drug release of the traditional antibacterial means is avoided, the induction effect of the stent on bone regeneration is enhanced, and the infection risk in the repair process is reduced.

The utility model discloses a positioning device for processing an artificial bone scaffold, which comprises a base, and a power component for driving a device to perform clamping motion is arranged on the base; the two clamping plates are symmetrically distributed in the base, the interiors of the two clamping plates are connected with rotating shafts through bearings, the rotating shafts and the rotating disc are installed together, the angle of the rotating shafts is controlled by rotating the rotating disc, and finally the machining angle of the support is controlled; a plurality of limiting holes which are annularly distributed are formed in a clamping plate, a limiting rod in a rotating disc can limit a limiting disc at multiple positions, the fixing angles of a support are more, machining of the support is facilitated, the support is clamped through cooperation of a first moving frame and a clamping semi-ring, the angle of the clamping semi-ring is adjustable, and the clamping efficiency is improved. And meanwhile, the positions of the first moving frame and the second moving frame are linked through gas in a three-way groove, so that the supports with the different shapes can be clamped conveniently.

The invention relates to the technical field of bone scaffold materials, and discloses a preparation method and application of a pre-vascularized bone repair scaffold, the preparation method of the pre-vascularized bone repair scaffold comprises the following steps: firstly, obtaining a porous scaffold and carrying out sulfonation treatment to obtain a sulfonated scaffold; then preparing a mixed solution containing gelatin and an extracellular matrix, and adding mesenchymal stem cells and / or endothelial cells to obtain a cell mixed preparation; and finally, contacting the cell mixed preparation with the sulfonated scaffold, and culturing to obtain the sulfonated scaffold. The pre-vascularized bone repair scaffold prepared by the preparation method disclosed by the invention simultaneously has excellent angiogenesis promoting capability and osteogenesis induction characteristic in vitro, and realizes vascularization-ossification synergistic interaction through a positive feedback loop mediated by a paracrine signal network; and a new thought and a new method are expected to be developed for clinical application of large-segment bone defect repair.

The application discloses a kind of biomimetic silk fibroin cartilage scaffolds for cranium defect repair, belong to biomedical engineering technical field.To solve the problem of single structure, low bone formation efficiency and mismatching of mechanical properties and degradation rate of existing cranium repair scaffold, the application provides a kind of scaffold, which includes in order from outside to inside: biomimetic periosteum layer, bone conduction and vascularization layer and cartilage induction layer.Biomimetic periosteum layer is dense nanofiber membrane structure, and plays the role of physical barrier and osteoinduction;Bone conduction and vascularization layer is gradient porous structure with gradually decreasing aperture from outside to inside, aiming to guide the ordered growth of blood vessels and cells, and promote rapid vascularization;Cartilage induction layer is sponge-like microporous structure, used for forming stable cartilage template and starting endochondral ossification.The application simulates the natural bone healing process through structural biomimicry and functional zoning, synergistically promotes vascularization and osteogenesis, and realizes efficient biological repair of cranium defect.

The utility model discloses a 3D printing hydrogel cartilage scaffold performance detection device, and relates to the technical field of hydrogel cartilage scaffold performance detection, the 3D printing hydrogel cartilage scaffold performance detection device comprises a performance detection device main body, the top of the performance detection device main body is uniformly provided with three storage grooves, a silica gel block is detachably mounted at the bottom of the inner wall of a storage groove formed in the performance detection device body, a working plate is detachably mounted at the top of the silica gel block, and a hydraulic push rod is detachably mounted on one side of the top of the working plate. According to the 3D printing hydrogel cartilage scaffold performance detection device, the arranged clamping plates drive the silica gel pads to be matched with the clamping seats to clamp to-be-detected printing hydrogel cartilage scaffolds of different sizes, the application range is wider, and the practicability is higher; the 3D printing hydrogel cartilage scaffold is fully detected by utilizing the reciprocating motion of the hydraulic push rod, the detection result is more reliable, and the hydraulic push rod on the working plate is damped through the cooperation of the arranged silica gel block and the fixed socket and the insertion rod.

The invention discloses a preparation method of porous ceramic with a non-uniform fusion structure, which comprises the following steps: step 1, constructing a fusion function, and determining parameters of the fusion function; 2, preparing a wax pattern negative template; step 3, preparing ceramic slurry; step 4, dipping and freezing; step 5, low-pressure drying; and 6, sintering to obtain the TPMS structure porous ceramic. The invention further discloses the porous ceramic with the non-uniform fusion structure. The invention further discloses application of the porous ceramic with the non-uniform fusion structure in the field of bionic bone scaffolds. According to the method, the fusion function is constructed by fusing the G-shaped function and the TPMS function, so that non-uniform fusion of various hole patterns is realized; the mode of wax negative template freezing casting and indirect forming is adopted, the design freedom degree of a complex structure is reserved, meanwhile, the problems of resolution limitation, shrinkage deformation, supporting difficulty and the like during direct 3D printing of the porous ceramic are solved, and the use effect is good.

The present invention provides an integrated design and manufacturing technology for a bone cartilage biomimetic gradient scaffold. The invention includes a functionally graded integrated bone cartilage biomimetic gradient scaffold, a transition structure for achieving graded transition between hard bone and cartilage tissue, and a biomimetic cartilage formation and post-processing method. The integrated bone cartilage biomimetic gradient scaffold is a porous interconnected ceramic structure that can be manufactured through integrated additive manufacturing. The transition structure is a special structure for firmly connecting hard bone and cartilage. The biomimetic cartilage formation and post-processing method involves performing special treatment on the 3D-printed bone scaffold to form biomimetic cartilage, thereby improving the strength of the cartilage layer and preventing cartilage damage caused by joint friction. The bone cartilage biomimetic scaffold structure proposed by the present invention has effective functional grading to mimic natural bone cartilage tissue, which is beneficial for preventing stress shielding and can be manufactured in one piece using 3D additive manufacturing technology.

The invention discloses a preparation method and application of a functional degradable soybean oil-based bionic porous composite bone scaffold. The method comprises the following steps: taking epoxidized soybean oil acrylate as a bio-based base material, compounding isobornyl methacrylate to improve the defects of high viscosity and low photosensitivity of the bio-based base material, adding a photoinitiator containing ethyl 2, 4, 6-trimethylbenzoyl phenyl phosphonate and phenyl bis (2, 4, 6-trimethylbenzoyl) phosphine oxide, compounding, and carrying out aging treatment to obtain base resin; mixing calcium lignosulphonate into the base resin to prepare composite photosensitive resin, and printing a triple-period extremely-small curved surface structure through a digital light processing technology and a 3D printing technology to finally obtain the functional degradable soybean oil-based bionic porous scaffold. The scaffold has calcium lignosulphonate-mediated controllable photo-thermal response behaviors of near-infrared light illumination regulation and bone scaffold component dependence under the condition of simulating the in-vivo environment of a human body, so that a controllable photo-thermal response-mediated remote shape memory function is realized, and meanwhile, a mild, safe and controllable photo-thermal curative effect can also be realized. Besides, calcium lignosulphonate promotes degradation of the composite scaffold by preferentially dissolving and generating a microporous structure on the surface of a matrix while improving the hydrophilic performance of the scaffold, and continuously releases calcium ions, so that the scaffold shows enhanced biomineralization ability and promotes cell proliferation and osteogenic differentiation. The research provides a solution with potential for the soybean oil-based biological photosensitive material in the digital light processing preparation technology and the multi-functional application of the bionic porous bone scaffold so as to deal with complex and irregular bone defects.

The application discloses a preparation method of non-uniform fusion structure porous ceramic, comprising the following steps: step one, constructing a fusion function and determining parameters of the fusion function; step two, preparing a wax negative template; step three, configuring ceramic slurry; step four, impregnating and freezing; step five, low-pressure drying; and step six, sintering, so as to obtain TPMS structure porous ceramic. The application also comprises non-uniform fusion structure porous ceramic. The application also comprises application of the non-uniform fusion structure porous ceramic in the field of biomimetic bone scaffolds. The application constructs a fusion function by fusing a G-shaped function and a TPMS function, realizes non-uniform fusion of various pore types, adopts a wax negative template freezing casting and an indirect forming mode, retains the design freedom of a complex structure, solves problems such as resolution limitation, shrinkage deformation and support difficulty that are faced by direct 3D printing of porous ceramic, and has good use effect.