Biotic bone tissue engineering stent and its preparation method

A tissue engineering scaffold and bionic bone technology, which is applied in the field of biomedical materials and tissue engineering, can solve the problems of solid particle settlement, uneven particle distribution, and difficult calcium phosphate materials, etc., achieve uniform distribution of components, improve mechanical strength, and improve Effects of Biocompatibility and Osteoconductivity

Inactive Publication Date: 2007-02-14
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to physical mixing, it is not easy to obtain a very uniform distribution of calcium phosphate materials, and during the cooling process of thermally induced phase separation, solid particles will settle, which will also cause uneven particle distribution.

Method used

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  • Biotic bone tissue engineering stent and its preparation method
  • Biotic bone tissue engineering stent and its preparation method
  • Biotic bone tissue engineering stent and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] a) Weigh 2g of chitosan, dissolve it in 50ml of 1.2% acetic acid aqueous solution, and stir well until it is completely dissolved. Take 12ml of 0.1mol / l Ca(NO 3 ) 2 Solution and 12ml of 0.06mol / l(NH 4 ) 2 HPO 4 The solution, under vigorous stirring, is added dropwise to the chitosan solution in order to ensure uniform dispersion as soon as possible after adding a small amount of solution each time, and to avoid the precipitation of chitosan caused by the increase of pH value caused by local concentration too high . Stir thoroughly to make it a completely homogeneous solution. Leave it at 4°C overnight or degas by vacuum pump.

[0028] b) Add the solution in step a) into the cavity 4 of the mold with a syringe, paying attention to slowly adding it to avoid bubbles. Cover the mold cover 1, put it vertically and slowly into liquid nitrogen, take it out after freezing for 12 hours, remove the top cover 1, bottom cover 2 and the stainless steel outer tube 3 after a little thawin...

Embodiment 2

[0037] a) Weigh 1g of chitosan and 1g of collagen, dissolve them in 50ml of 1.2% acetic acid aqueous solution, and stir well until they are completely dissolved. Take 12ml of 0.1mol / l Ca(NO 3 ) 2 Solution and 12ml of 0.1mol / l(NH 4 ) 2 HPO 4 The solution is added dropwise to the chitosan-collagen mixed solution under vigorous stirring to ensure uniform dispersion as soon as possible after adding a small amount of solution each time, and to avoid the chitosan caused by the increase in pH value caused by excessive local concentration. Of precipitation. Stir well to make it a completely homogeneous solution. Let stand overnight at 4°C or degas by vacuum pump.

[0038] b) Add the solution obtained in a) into the mold cavity 4 with a syringe, paying attention to slowly adding it to avoid generating bubbles. Cover the mold cover 1, place it vertically at -20°C, and take it out after freezing for 24 hours. After a little thawing, remove the top cover 1, bottom cover 2 and the stainless st...

Embodiment 3

[0046] a) Weigh 1g of chitosan, dissolve it in 50ml of 1.2% acetic acid aqueous solution, and stir well until it is completely dissolved. Add an appropriate amount of 2% gelatin aqueous solution to the chitosan solution, stir for 2 hours at 50°C and mix well, and take 12ml of 0.1mol / l Ca(NO 3 ) 2 Solution and 12ml of 0.05mol / l(NH 4 ) 2 HPO 4 The solution is added dropwise to the chitosan solution under vigorous stirring to ensure uniform dispersion as soon as possible after adding a small amount of solution each time, and to avoid the precipitation of chitosan caused by the increase in the pH value caused by the local concentration. . Stir well to make it a completely homogeneous solution. Let stand overnight at 4°C or degas by vacuum pump.

[0047] b) Add the solution obtained in a) into the mold cavity 4 with a syringe, paying attention to slowly adding it to avoid generating bubbles. Cover the mold cover 1, put it vertically and slowly into liquid nitrogen, take it out after fr...

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Abstract

A tissue-engineered bionic bone scaffold with high biocompatibility, bioactivity, degradability and osteoplastic induction has a three-layer structure composed of external degradable compact biofilm layer, central porous layer with less pore diameter for high mechanical strength, and internal porous layer with big pore diameter for transferring nutrients. Said central and internal layers are a composition consisting of in-situ formed nano-phase Ca-P salt and degradable biologic material.

Description

Technical field [0001] The invention relates to a bionic bone tissue engineering scaffold and a preparation method thereof, and belongs to the technical field of biomedical materials and tissue engineering. Background technique [0002] The treatment of bone defect is one of the common clinical problems. The bone defect brings great inconvenience to the patient's life and seriously affects daily life. Common clinical treatment methods such as autologous bone transplantation and allogeneic bone transplantation have the risk of limited donors, immune rejection and disease transmission. Using tissue engineering methods to prepare bone substitutes to promote bone regeneration has become a new method for bone defect treatment. The main method of tissue engineering is to absorb and inoculate normal tissue cells cultured in vitro on a porous scaffold material that has good biocompatibility and can be degraded and absorbed by the body, and then the cell material composite is implanted in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/54A61L27/56A61L27/58
Inventor 张秀芳孔丽君公衍道敖强王爱军龚楷
Owner TSINGHUA UNIV
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