39 results about "Tissue engineer" patented technology

The preparation method comprises the following steps: (1) fish skin pretreatment: (2) gas foaming treatment: weighing sodium borohydride with a certain molar concentration, dissolving the sodium borohydride in a phosphate buffer solution with a certain concentration, stirring until bubbles in the solution are obviously generated and most of solids are dissolved, and then, carrying out gas foaming treatment; putting the cleaned fish skin tissues in the step (2), and performing foaming treatment; (3) cleaning treatment; and (4) protection and storage. The gas foaming technology is innovatively adopted for decellularization treatment, the interface stripping effect between cells and a matrix is remarkably enhanced on the premise that the collagen three-dimensional structure and the scaffold morphology integrity are not damaged, and the method is suitable for the decellularization pretreatment process of soft tissue (such as fish skin and placenta) with a compact structure or cell-intensive type. The development of a biomedical material, especially a tissue engineering scaffold technology, is promoted, and the preparation method has a wide application prospect.

The invention relates to a tissue engineering trachea, which combines an additive manufacturing technology with a biological material, simulates the form and the function of a natural gas pipe, designs a cartilage-fiber alternating ring structure and a bionic fiber band structure, and designs a unique blood supply channel in a fiber section. The trachea fiber section is composed of hydrogel with a hierarchical porous structure, and a circumferential blood vessel channel and a radial blood vessel pore channel are designed in the hydrogel so as to promote ingrowth and growth of new blood vessels and optimize the blood supply reconstruction process. The tracheal cartilage section is made into a C-shaped cartilage ring with cartilage cells through a biological printing technology, and necessary mechanical support is provided. The fiber segment and the cartilage segment are effectively integrated through chemical bonds of functional groups to ensure stable connection of the two. Partitioned construction of fibers and cartilage is achieved through the biological 3D printing technology, the bionic tissue engineering trachea is formed in a modular construction-integrated assembly mode, it is verified that repairing and reconstruction of multiple types of tissue bionic tracheas can be achieved, and an innovative solution is provided for tracheal injuries.

The invention relates to a tissue engineering articular cartilage simulating a four-layer structure of a natural cartilage and an in-situ 3D printing method of the tissue engineering articular cartilage, the structure and component characteristics of'surface layer-transitional layer-radiation layer-calcification layer 'of the natural cartilage are re-carved through precise layered modeling and layer-specific bio-ink, and the articular cartilage is obtained by means of an optimized in-situ 3D printing process. The tissue engineering cartilage which is matched with dissection of a defect area and consistent with mechanical and physiological functions of natural cartilage is directly constructed in an articular cavity. Besides, the in-vitro induced differentiation step of the BMSCs is reduced, the risk of cell mutation is reduced, meanwhile, the long-term integration of the repaired tissue and the host cartilage is improved, and the problem that the long-term effectiveness of clinical repair is insufficient is solved.

The invention relates to a gradient scaffold material based on particle gel as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) constructing a shaping framework; (2) respectively preparing a plurality of hydrogel particles with different properties; and (3) respectively injecting the hydrogel particles with different properties into the shaping frame, and carrying out in-situ crosslinking on the hydrogel particles to prepare the gradient scaffold material. The scaffold material prepared by the method has the characteristic that the composition, the structure and the mechanical property are continuously adjustable in space, and the scaffold also has good biocompatibility and a penetrating porous structure, so that inward migration, proliferation and directional differentiation of cells are facilitated, and the scaffold material has good application prospects. The method has important application in the fields of bone-cartilage, tendon-bone, skin and other gradual change tissue engineering interface repair and the like.

The invention provides a tissue-engineered red blood cell as well as a preparation method and application thereof. The tissue-engineered red blood cell is prepared by the following method: taking red blood cells, culturing the red blood cells in a culture medium containing recombinant erythropoietin, then adding TAT peptide lactoferrin nanoparticles, and incubating to obtain the tissue-engineered red blood cell. The preparation method comprises the following steps: firstly, adding TAT peptide in the construction process of LF-IONPs to prepare TAT peptide lactoferrin nanoparticles, and then incubating the TAT peptide lactoferrin nanoparticles with erythrocytes in a culture system containing recombinant erythropoietin to obtain the tissue-engineered erythrocytes. Therefore, when the TAT peptide lactoferrin nanoparticles enter red blood cells through phagocytosis, the activity, high survival rate and normal function of the red blood cells are ensured, so that the prepared tissue-engineered red blood cells have brittleness, form, in-vivo tracing and half-life period similar to those of normal red blood cells, thereby having the basis of in-vivo medication.

The invention relates to the technical field of biological medicine and tissue engineering, in particular to a tissue engineering blood vessel and a preparation method and application thereof. The tissue engineering blood vessel is obtained by modifying interleukin 24 (IL-24) on the surface of an acellular blood vessel, after the tissue engineering blood vessel is transplanted into a body, the number of CD31 + and CD34 + cells can be remarkably increased, and infiltration of M2 type macrophages (CD163 +) on the surface of the blood vessel is remarkably increased; the expression of HIF-1 alpha (hypoxia marker) and MMP9 is obviously reduced; the expression of the nerve specific protein S-100 is obviously increased, thrombosis and intimal hyperplasia are effectively inhibited, and the patency rate of transplanted blood vessels is obviously increased.

The invention provides a dental pulp stem cell exosome-loaded acellular tissue engineering nerve conduit as well as a preparation method and application thereof, relates to the technical field of biomedical engineering and nerve regeneration medicine, and aims to solve the problems of poor mechanical property, low bionic degree, high degradation rate, insufficient biological activity and the like of an existing nerve conduit. The technical key point of the invention is as follows: the dental pulp stem cell exosome-loaded acellular tissue engineering nerve conduit is provided, and the nerve conduit is obtained by loading a nanofiber conduit with enhanced function of over-expressing let-7i-5p, the porous nanofiber catheter is a porous catheter which is obtained after a polyvinyl alcohol (PVA)-chitosan (CS)-graphene (G) electrostatic spinning membrane is subjected to pipe reeling and crosslinking, and the nucleotide sequence of let-7i-5p in DPSC-Exo is shown as SEQ NO.1. The invention further discloses a preparation method of the porous nanofiber catheter.

The present inventions is to produce a tissue engineered tube including a smooth muscle layer and an endothelial cell layer derived from stem cells. It is possible to produce a tissue engineered tube that generates physiological contraction and relaxation reactions by producing a tissue engineered tube using smooth muscle cells derived from stem cells at least three times or more as those used in a method of the related art, and using endothelial cells derived from stem cells. Since an artificial blood vessel produced using iPS cells derived from a Werner syndrome patient indicates being an artificial blood vessel reflecting a pathological condition, tissue engineered tubes reflecting pathological conditions of various diseases can be produced using iPS cells. The tissue engineered tube can be used in pharmaceutical screening and transplantation.

Engineered tissue models based on three-dimensional (3D) scaffolds, also referred to herein as tissue-engineered 3D models, can be used as in vitro diagnostic and drug screening tools for predicting, preventing and / or treating cancer metastases.

The present invention relates to the technical field of tissue-engineered blood vessels, and specifically relates to an engineered exosome that can be applied to autologous blood vessels and small-caliber tissue-engineered blood vessels for anti-calcification, and a preparation method and application thereof. An engineered exosome has an E7 mesenchymal stem cell affinity peptide modified on its surface; and lncRNA-ANCR is encapsulated inside it. This engineered exosome can target Gli1+ cells in vivo and regulate their differentiation trend, and can reverse vascular calcification caused by chronic kidney disease; the small-caliber tissue-engineered blood vessels modified by it can accelerate the reconstruction of the smooth muscle layer of vascular grafts, have mechanical strength similar to that of natural blood vessels in vivo, and effectively inhibit the calcification of vascular grafts under normal and diabetic conditions by regulating the differentiation of smooth muscle progenitor cells. This solution can solve the technical problems of low efficiency of smooth muscle reconstruction in tissue-engineered blood vessels or autologous blood vessels and easy calcification of blood vessels, and at the same time meet the requirements of early cell reconstruction and long-term inhibition of calcification.

The invention provides a method and a system for constructing a Bouligand tissue engineering auricle. The method for constructing the Bouligand tissue engineering auricle comprises the following steps: acquiring an auricle digital model; modeling by using the auricle digital model to obtain a nested auricle model; the nested auricle model is processed, and a Bouligand structure auricle model is obtained; and carrying out 3D printing on the auricle model with the Bouligand structure, so as to obtain the bionic tissue engineering auricle with the Bouligand structure. According to the technical scheme, the anisotropy of the natural auricle is simulated, so that the tissue engineering auricle capable of resisting stress and accurately maintaining the shape in vivo is obtained.

The invention relates to a construction method of tissue engineering corneal epithelial cells. The construction method comprises the following steps: obtaining corneal limbus tissue blocks; digesting the tissue block, and separating to obtain epithelial cells of the tissue block; the method comprises the following steps: attaching epithelial cells to ultrathin amniotic membrane-umbilical cord derived mesenchymal stem cells, culturing in an SHEM culture medium, changing the SHEM culture medium once every two days, culturing for a period of time, and culturing the epithelial cells for a period of time by using a gas-liquid interface culture method to promote the multi-layer growth of the epithelial cells, thereby obtaining the tissue engineering corneal epithelial cells. Through a controllable preparation scheme, the amniotic membrane carrier with a certain thickness is obtained so as to increase the transparency of the amniotic membrane carrier, meanwhile, the matrix surface of the amniotic membrane carrier is inoculated with MSC, the healthy corneal limbus matrix microenvironment of a human body is simulated, damaged corneal limbus tissue / corneal epithelium is better repaired or regenerated, and the ocular surface microenvironment is improved. The produced ultrathin amnion combined mesenchymal stem cell carrier can obviously improve the effect of a tissue engineering corneal epithelium transplantation operation.

This invention relates to the field of intelligent code rewriting and discloses a code rewriting method for a domestic IT innovation platform based on a self-iterative model. The method operates in a code rewriting system comprising a basic database and a multi-link space. The multi-link space includes a rewriting space, a splitting space, and a virtual space. The method includes acquiring the target code, parsing the logical chain of the target code based on a neural symbolic self-iterative model, splitting the target code into sub-code segments, performing error factor analysis on the sub-code segments, executing the rewriting in the rewriting space, running the rewritten code in the virtual space, cross-validating the code execution results with the expected rewriting results, and controlling the asynchronous execution of code splitting, code rewriting, and virtual execution through an asynchronous clock and state signal mechanism to ensure that the execution results in the virtual space are consistent with the expected results, and outputting the rewritten code.

The present invention relates to the technical field of preparation of medical tissue engineering scaffold materials, and discloses a biodegradable medical scaffold material and its application, which comprises the following components by weight percentage: 50%-65% polysuccinate, 3%-6% dopamine group, 15%-25% nano magnesium calcium silicate, 10%-20% polycaprolactone and 4%-5% ammonium bicarbonate, and also discloses an application of the biodegradable medical scaffold material in bone tissue repair, wherein the scaffold material is used to fill bone defects or fracture sites. The degradation performance is optimized by combining polycaprolactone and nano magnesium calcium silicate with a polysuccinate matrix, the mechanical properties are improved by a dynamic covalent network and dopamine groups, and the biological functionality is enhanced by modifying dopamine groups with RGD peptides, and the drug sustained release and porous structure design are combined to provide a high-performance scaffold material for bone tissue repair that has both mechanical support, degradation controllability and osteoconductivity.

The present invention relates to the technical field of medical materials, and provides a tissue engineering biomimetic tracheal stent with a microenvironment regulation function and a preparation method thereof. The present invention is based on a non-woven fabric reinforced three-dimensional silk fibroin sponge scaffold, on which chondrocytes or quercetin are loaded, and then the obtained cartilage rings and quercetin rings are alternately stacked to form the tissue engineering biomimetic tracheal stent of the present invention. The tissue engineering biomimetic tracheal stent provided by the present invention can stably exist in a living body, and is similar to natural tissue in structure and function. During the pre-vascularization process, it can produce a transmural vascular structure similar to a physiological trachea, thereby providing nutrition and metabolic support for the inner structure of the graft. In addition, quercetin can regulate the immune microenvironment by promoting the polarization of macrophages to an anti-inflammatory phenotype, broadening the application scope of tissue engineering trachea and promoting its clinical application.

The present invention relates to a bioink for 3D printing, the preparation method, and the usage. Such bioink is a gel made of α-zein, porogen by 0-10% of the weight of zein, ethanol and water. The preparation method consists of: 10-50% zein is dissolved into an aqueous solution containing 40-90% (v / v) of ethanol, then, the porogen by 0-10% of the weight of zein is added, and then, this solution is allowed to stand at 5-95° C. for 1-10 days, or stirred for 30 min-24 hours, and thus, the bioink for 3D printing can be obtained. The conditions applied to prepare such bioink are mild and the method adopted is easy to operate, in addition, the said bioink for 3D printing has good mechanical properties and biocompatibility, which can be applied in the field of biomedicine for preparing tissue engineered substitutes and hemostatic materials by 3D printing at room temperature.

The invention relates to a preparation method and application of a neural tissue engineering blood vessel. The preparation method comprises the following steps: preparing neural stem cell / neural precursor cell neural spheres; preparing a gelatin sponge into a hollow cylinder, adding neural spheres, and incubating to prepare a neural gelatin sponge; and penetrating the decellularized artificial blood vessel or other tissue engineering blood vessels into the neurogelatin sponge for incubation and culture to obtain the neurotized tissue engineering blood vessel. The vascular graft with a nerve secretion function is constructed by optimizing preparation and dynamic culture parameters of neural spheres and combining a cooperative processing technology of a gelatin sponge stent and an acellular vascular matrix, and is used for repairing coronary artery, carotid artery and peripheral vascular defects; the method has the advantages that rapid re-endothelialization, innervation and anti-inflammatory microenvironment formation of transplanted blood vessels can be promoted, repair and fusion of the blood vessels can be accelerated, a new neurotization scheme is provided for tissue engineering blood vessels, and a new method is provided for solving the difficult problems of reconstruction and long-term stability of transplanted blood vessels clinically.

The present invention is directed to a method for manufacturing a human cell derived tissue engineered matrix (hTEM) for an implant application, said hTEM based on genetically modified cells expressing an inducible apoptotic initiator gene. The invention further is directed towards an hTEM manufactured by the respective method and to the respective apoptosis-assisted decellularization process.

A method is provided for non-invasively predicting characteristics of one or more cells and cell derivatives. The method includes training a machine learning model using at least one of a plurality of training cell images representing a plurality of cells and data identifying characteristics for the plurality of cells. The method further includes receiving at least one test cell image representing at least one test cell being evaluated, the at least one test cell image being acquired noninvasively and based on absorbance as an absolute measure of light, and providing the at least one test cell image to the trained machine learning model. Using machine learning based on the trained machine learning model, characteristics of the at least one test cell are predicted. The method further includes generating, by the trained machine learning model, release criteria for clinical preparations of cells based on the predicted characteristics of the at least one test cell.

The present invention relates to a method for isolating and culturing tissue-resident uPAR+ / Nestin+ stem cells, and a use thereof, and provides a method for isolating, on the basis of uPAR-plasmin activity, tissue-resident stem cells present in solid tissues, wherein the uPAR-plasmin activity of stem cells has been suggested to have a close relationship with stem cell growth, migration ability, physiological activity and differentiation ability, and thus can be used in a method for isolating high-potency stem cells, the uPAR+ stem cells isolated from solid tissues are applicable to the production of cell therapeutic agents, tissue engineering therapeutic agents, and novel biopharmaceuticals using secretions including exosomes, and thus have high industrial applicability, and the present invention is applicable to fundamental cell biology and molecular biology research and novel drug development research that relate to the cell division, migration, growth, and differentiation of tissue-resident stem cells.

The present application relates to the technical field of tissue engineering scaffold, and especially relates to a tissue engineering tubular scaffold and a manufacturing method thereof, which comprises: a tubular support framework, a plurality of holes are arranged on the tube wall of the tubular support framework, the tubular support framework is etched and surface modified by low-temperature plasma; a hydrogel coating layer, which comprises an axially extending hydrogel coating inner layer and a hydrogel coating outer layer, the tubular support framework is arranged between the hydrogel coating inner layer and the hydrogel coating outer layer, and the hydrogel coating layer completely covers the tubular support framework; and the tubular support framework is used for supporting the hydrogel coating inner layer and the hydrogel coating outer layer. By being arranged in this way, the tissue engineering tubular scaffold of the present application is beneficial to the culture of tubular tissues in vitro and meets the requirements of biological performance and mechanical performance after being implanted into the body, and solves the problems of poor form retention ability, insufficient mechanical performance and poor bonding strength of different materials of the hydrogel 3D printing tissue engineering tubular scaffold.

The present invention discloses a method for constructing a fully biological small-caliber tissue-engineered blood vessel, and belongs to the field of bioengineering technology. This method combines biological 3D printing technology and stem cell in situ induction technology for blood vessel construction for the first time, and creatively proposes vascular bionics. Biological 3D printing is used to quickly prepare blood vessels to support customized caliber and length, with a number of vascular cells close to physiological density, internal cells have a good growth environment, rich extracellular matrix types, and excellent mechanical properties. In situ induction technology obtains a large number of vascular smooth muscle cells; mechanical stimulation is applied, and the extracellular matrix is ​​further reshaped to obtain mechanical properties close to natural blood vessels; after in vitro endothelialization, fully biological small-caliber tissue-engineered blood vessels are obtained. The fully biological small-caliber tissue-engineered blood vessels constructed by this method can be used clinically for fully biological small-caliber tissue-engineered blood vessels for peripheral vascular replacement, arteriovenous fistula, and coronary artery bypass.

The utility model belongs to the technical field of cell mechanics biology experimental devices for health and rehabilitation engineering, and relates to a microfluidic device for endothelialization evaluation of a tissue engineering valve, which comprises a microfluidic chip, peripheral equipment, a signal acquisition and processing system and a feedback system. According to the utility model, the biomechanical principle and the micro-fluidic chip technology are ingeniously combined, the control system, the mechanical loading device and the micro-fluidic chip are connected to construct a micro-fluidic endothelial cell culture cavity and a peripheral system, and the real-time accurate control of the blood flow of the aortic valve is realized through the PID control system; shear stress applied to a tissue engineering valve endothelial cell culture area is generated, so that the real stress condition of the tissue engineering valve endothelial cells is simulated, and the method is used for analyzing and researching the influence rule of mechanical loading on the tissue engineering valve endothelial cells.

Disclosed are tissue engineered graft materials useful as scaffolds for blood vessel grafts in various surgical procedures and methods of making such scaffolds.

The application discloses a small-bore tissue engineering blood vessel and a preparation method thereof. The preparation method of the small-bore tissue engineering blood vessel comprises the following steps: placing an electrostatic spinning polyethylene glycol tubular film in a columnar sandwich mold layer; mixing seed cells and a first solution to prepare a seed cell-protein mixed solution, and injecting the seed cell-protein mixed solution into the columnar sandwich mold layer containing the polyethylene glycol tubular film to form a composite gel; after the seed cell-protein-polymer composite gel is statically cultured to be stable, the seed cell-protein-polymer composite gel is continuously dynamically perfusion cultured under a simulated body arterial pulsatile flow pressure condition to obtain a tissue engineering blood vessel composed of seed cells and extracellular matrix; and the tissue engineering blood vessel is subjected to a decellularization method to remove cell components in a tube wall, so that a tissue engineering blood vessel composed of only extracellular matrix is obtained.

The invention provides a magnesium oxide-loaded asymmetric short fiber aerogel scaffold as well as a preparation method and application thereof, and belongs to the technical field of tissue engineering materials. The preparation method comprises the following steps: adding MgO nanoparticles into a PLLA / Gelatin solution, and carrying out electrostatic spinning, crushing, freeze drying and crosslinking to obtain the porous short fiber aerogel scaffold (GP-coated MgO). The GP-coated MgO aerogel stent has a three-dimensional short fiber structure, and can release loaded MgO nanoparticles in a progressive and continuous manner while wound exudate is slowly absorbed. In-vivo and in-vitro experiments prove that the GP-coated MgO aerogel scaffold has biocompatibility and is very effective in the aspects of inhibiting inflammatory response and slowing down cell senescence. In addition, the GP-coated MgO significantly accelerates vascular reconstruction and epithelization, and promotes wound repair at the skin defect position of a diabetic rat.

The invention relates to a preparation method of a multi-response programmable self-disintegration structural body, a structural body formed by an outer liquid absorption layer and an inner temperature-sensitive response layer activates a response structural layer when local liquid exudes or the temperature rises, structural disintegration and sequential release of functional components are induced, the disintegration time range of the structural body is 48-72 hours, and the structural body is not prone to disintegration. The response process can be adjusted according to an actual application scene; the structural body prepared by the method is relatively high in biological safety, the inner-layer drug release is accurate and controllable, the outer-layer protects the wound surface and provides a micro-wet environment, and the structural body has temperature-sensitive intelligent responsiveness, adapts to the temperature change of the wound surface and can be applied to the wound surface. The preparation method is suitable for the following biomedical scenes: chronic wound dressings (diabetic foot and bedsore), postoperative sustained-release films, tissue engineering scaffold materials, tumor local controlled-release film carriers and the like.

The present application belongs to the technical field of hydrogel, and particularly relates to a hydrogel material with active oxygen responsiveness and a preparation method thereof, and further relates to an antibacterial / anti-ferric death bone repair composite scaffold comprising a tissue engineering porous scaffold material and the hydrogel material with active oxygen responsiveness filled in the tissue engineering porous scaffold material, in particular to an antibacterial / anti-ferric death bone repair composite scaffold comprising a 3D-printed polycaprolactone / bioglass porous scaffold material and the hydrogel material with active oxygen responsiveness filled in the porous scaffold material and a preparation method thereof, and further relates to application of the antibacterial / anti-ferric death bone repair composite scaffold with active oxygen responsiveness as described above to treatment of infectious bone defects.

The application relates to a biological 3D printed tissue engineering trachea and a construction method and application thereof, namely a cartilage / vascularized fiber tissue alternating tissue engineering trachea. The construction method comprises the following steps: firstly, cartilage specific biological ink is prepared by wrapping cartilage cells with cartilage tissue specific hydrogel; fiber specific biological ink is prepared by wrapping fibroblasts with fiber tissue specific hydrogel; then, through a double-needle extrusion type biological 3D printing technology, alternating printing is realized to form a ring-shaped tubular structure, and a cartilage / fiber tissue alternating tubular structure is constructed through multi-layer accumulation molding; further, vascularization of the fiber tissue is realized through a certain culture mode, and finally, a relatively mature cartilage / vascularized fiber tissue alternating tissue engineering trachea is obtained. The application also provides application of the cartilage / vascularized fiber tissue alternating tissue engineering trachea in tracheal defect repair.