31 results about "Tissue engineer" patented technology

The preparation method comprises the following steps: (1) fish skin pretreatment: (2) gas foaming treatment: weighing sodium borohydride with a certain molar concentration, dissolving the sodium borohydride in a phosphate buffer solution with a certain concentration, stirring until bubbles in the solution are obviously generated and most of solids are dissolved, and then, carrying out gas foaming treatment; putting the cleaned fish skin tissues in the step (2), and performing foaming treatment; (3) cleaning treatment; and (4) protection and storage. The gas foaming technology is innovatively adopted for decellularization treatment, the interface stripping effect between cells and a matrix is remarkably enhanced on the premise that the collagen three-dimensional structure and the scaffold morphology integrity are not damaged, and the method is suitable for the decellularization pretreatment process of soft tissue (such as fish skin and placenta) with a compact structure or cell-intensive type. The development of a biomedical material, especially a tissue engineering scaffold technology, is promoted, and the preparation method has a wide application prospect.

The invention relates to a tissue engineering trachea, which combines an additive manufacturing technology with a biological material, simulates the form and the function of a natural gas pipe, designs a cartilage-fiber alternating ring structure and a bionic fiber band structure, and designs a unique blood supply channel in a fiber section. The trachea fiber section is composed of hydrogel with a hierarchical porous structure, and a circumferential blood vessel channel and a radial blood vessel pore channel are designed in the hydrogel so as to promote ingrowth and growth of new blood vessels and optimize the blood supply reconstruction process. The tracheal cartilage section is made into a C-shaped cartilage ring with cartilage cells through a biological printing technology, and necessary mechanical support is provided. The fiber segment and the cartilage segment are effectively integrated through chemical bonds of functional groups to ensure stable connection of the two. Partitioned construction of fibers and cartilage is achieved through the biological 3D printing technology, the bionic tissue engineering trachea is formed in a modular construction-integrated assembly mode, it is verified that repairing and reconstruction of multiple types of tissue bionic tracheas can be achieved, and an innovative solution is provided for tracheal injuries.

The invention relates to a tissue engineering articular cartilage simulating a four-layer structure of a natural cartilage and an in-situ 3D printing method of the tissue engineering articular cartilage, the structure and component characteristics of'surface layer-transitional layer-radiation layer-calcification layer 'of the natural cartilage are re-carved through precise layered modeling and layer-specific bio-ink, and the articular cartilage is obtained by means of an optimized in-situ 3D printing process. The tissue engineering cartilage which is matched with dissection of a defect area and consistent with mechanical and physiological functions of natural cartilage is directly constructed in an articular cavity. Besides, the in-vitro induced differentiation step of the BMSCs is reduced, the risk of cell mutation is reduced, meanwhile, the long-term integration of the repaired tissue and the host cartilage is improved, and the problem that the long-term effectiveness of clinical repair is insufficient is solved.

The invention provides a tissue-engineered red blood cell as well as a preparation method and application thereof. The tissue-engineered red blood cell is prepared by the following method: taking red blood cells, culturing the red blood cells in a culture medium containing recombinant erythropoietin, then adding TAT peptide lactoferrin nanoparticles, and incubating to obtain the tissue-engineered red blood cell. The preparation method comprises the following steps: firstly, adding TAT peptide in the construction process of LF-IONPs to prepare TAT peptide lactoferrin nanoparticles, and then incubating the TAT peptide lactoferrin nanoparticles with erythrocytes in a culture system containing recombinant erythropoietin to obtain the tissue-engineered erythrocytes. Therefore, when the TAT peptide lactoferrin nanoparticles enter red blood cells through phagocytosis, the activity, high survival rate and normal function of the red blood cells are ensured, so that the prepared tissue-engineered red blood cells have brittleness, form, in-vivo tracing and half-life period similar to those of normal red blood cells, thereby having the basis of in-vivo medication.

The invention relates to the technical field of biological medicine and tissue engineering, in particular to a tissue engineering blood vessel and a preparation method and application thereof. The tissue engineering blood vessel is obtained by modifying interleukin 24 (IL-24) on the surface of an acellular blood vessel, after the tissue engineering blood vessel is transplanted into a body, the number of CD31 + and CD34 + cells can be remarkably increased, and infiltration of M2 type macrophages (CD163 +) on the surface of the blood vessel is remarkably increased; the expression of HIF-1 alpha (hypoxia marker) and MMP9 is obviously reduced; the expression of the nerve specific protein S-100 is obviously increased, thrombosis and intimal hyperplasia are effectively inhibited, and the patency rate of transplanted blood vessels is obviously increased.

The invention provides a dental pulp stem cell exosome-loaded acellular tissue engineering nerve conduit as well as a preparation method and application thereof, relates to the technical field of biomedical engineering and nerve regeneration medicine, and aims to solve the problems of poor mechanical property, low bionic degree, high degradation rate, insufficient biological activity and the like of an existing nerve conduit. The technical key point of the invention is as follows: the dental pulp stem cell exosome-loaded acellular tissue engineering nerve conduit is provided, and the nerve conduit is obtained by loading a nanofiber conduit with enhanced function of over-expressing let-7i-5p, the porous nanofiber catheter is a porous catheter which is obtained after a polyvinyl alcohol (PVA)-chitosan (CS)-graphene (G) electrostatic spinning membrane is subjected to pipe reeling and crosslinking, and the nucleotide sequence of let-7i-5p in DPSC-Exo is shown as SEQ NO.1. The invention further discloses a preparation method of the porous nanofiber catheter.

The present inventions is to produce a tissue engineered tube including a smooth muscle layer and an endothelial cell layer derived from stem cells. It is possible to produce a tissue engineered tube that generates physiological contraction and relaxation reactions by producing a tissue engineered tube using smooth muscle cells derived from stem cells at least three times or more as those used in a method of the related art, and using endothelial cells derived from stem cells. Since an artificial blood vessel produced using iPS cells derived from a Werner syndrome patient indicates being an artificial blood vessel reflecting a pathological condition, tissue engineered tubes reflecting pathological conditions of various diseases can be produced using iPS cells. The tissue engineered tube can be used in pharmaceutical screening and transplantation.

Engineered tissue models based on three-dimensional (3D) scaffolds, also referred to herein as tissue-engineered 3D models, can be used as in vitro diagnostic and drug screening tools for predicting, preventing and / or treating cancer metastases.

This invention relates to the field of intelligent code rewriting and discloses a code rewriting method for a domestic IT innovation platform based on a self-iterative model. The method operates in a code rewriting system comprising a basic database and a multi-link space. The multi-link space includes a rewriting space, a splitting space, and a virtual space. The method includes acquiring the target code, parsing the logical chain of the target code based on a neural symbolic self-iterative model, splitting the target code into sub-code segments, performing error factor analysis on the sub-code segments, executing the rewriting in the rewriting space, running the rewritten code in the virtual space, cross-validating the code execution results with the expected rewriting results, and controlling the asynchronous execution of code splitting, code rewriting, and virtual execution through an asynchronous clock and state signal mechanism to ensure that the execution results in the virtual space are consistent with the expected results, and outputting the rewritten code.

The present invention relates to a bioink for 3D printing, the preparation method, and the usage. Such bioink is a gel made of α-zein, porogen by 0-10% of the weight of zein, ethanol and water. The preparation method consists of: 10-50% zein is dissolved into an aqueous solution containing 40-90% (v / v) of ethanol, then, the porogen by 0-10% of the weight of zein is added, and then, this solution is allowed to stand at 5-95° C. for 1-10 days, or stirred for 30 min-24 hours, and thus, the bioink for 3D printing can be obtained. The conditions applied to prepare such bioink are mild and the method adopted is easy to operate, in addition, the said bioink for 3D printing has good mechanical properties and biocompatibility, which can be applied in the field of biomedicine for preparing tissue engineered substitutes and hemostatic materials by 3D printing at room temperature.

The invention relates to a preparation method and application of a neural tissue engineering blood vessel. The preparation method comprises the following steps: preparing neural stem cell / neural precursor cell neural spheres; preparing a gelatin sponge into a hollow cylinder, adding neural spheres, and incubating to prepare a neural gelatin sponge; and penetrating the decellularized artificial blood vessel or other tissue engineering blood vessels into the neurogelatin sponge for incubation and culture to obtain the neurotized tissue engineering blood vessel. The vascular graft with a nerve secretion function is constructed by optimizing preparation and dynamic culture parameters of neural spheres and combining a cooperative processing technology of a gelatin sponge stent and an acellular vascular matrix, and is used for repairing coronary artery, carotid artery and peripheral vascular defects; the method has the advantages that rapid re-endothelialization, innervation and anti-inflammatory microenvironment formation of transplanted blood vessels can be promoted, repair and fusion of the blood vessels can be accelerated, a new neurotization scheme is provided for tissue engineering blood vessels, and a new method is provided for solving the difficult problems of reconstruction and long-term stability of transplanted blood vessels clinically.

The present invention is directed to a method for manufacturing a human cell derived tissue engineered matrix (hTEM) for an implant application, said hTEM based on genetically modified cells expressing an inducible apoptotic initiator gene. The invention further is directed towards an hTEM manufactured by the respective method and to the respective apoptosis-assisted decellularization process.

A method is provided for non-invasively predicting characteristics of one or more cells and cell derivatives. The method includes training a machine learning model using at least one of a plurality of training cell images representing a plurality of cells and data identifying characteristics for the plurality of cells. The method further includes receiving at least one test cell image representing at least one test cell being evaluated, the at least one test cell image being acquired noninvasively and based on absorbance as an absolute measure of light, and providing the at least one test cell image to the trained machine learning model. Using machine learning based on the trained machine learning model, characteristics of the at least one test cell are predicted. The method further includes generating, by the trained machine learning model, release criteria for clinical preparations of cells based on the predicted characteristics of the at least one test cell.

The present invention relates to a method for isolating and culturing tissue-resident uPAR+ / Nestin+ stem cells, and a use thereof, and provides a method for isolating, on the basis of uPAR-plasmin activity, tissue-resident stem cells present in solid tissues, wherein the uPAR-plasmin activity of stem cells has been suggested to have a close relationship with stem cell growth, migration ability, physiological activity and differentiation ability, and thus can be used in a method for isolating high-potency stem cells, the uPAR+ stem cells isolated from solid tissues are applicable to the production of cell therapeutic agents, tissue engineering therapeutic agents, and novel biopharmaceuticals using secretions including exosomes, and thus have high industrial applicability, and the present invention is applicable to fundamental cell biology and molecular biology research and novel drug development research that relate to the cell division, migration, growth, and differentiation of tissue-resident stem cells.

The present application relates to the technical field of tissue engineering scaffold, and especially relates to a tissue engineering tubular scaffold and a manufacturing method thereof, which comprises: a tubular support framework, a plurality of holes are arranged on the tube wall of the tubular support framework, the tubular support framework is etched and surface modified by low-temperature plasma; a hydrogel coating layer, which comprises an axially extending hydrogel coating inner layer and a hydrogel coating outer layer, the tubular support framework is arranged between the hydrogel coating inner layer and the hydrogel coating outer layer, and the hydrogel coating layer completely covers the tubular support framework; and the tubular support framework is used for supporting the hydrogel coating inner layer and the hydrogel coating outer layer. By being arranged in this way, the tissue engineering tubular scaffold of the present application is beneficial to the culture of tubular tissues in vitro and meets the requirements of biological performance and mechanical performance after being implanted into the body, and solves the problems of poor form retention ability, insufficient mechanical performance and poor bonding strength of different materials of the hydrogel 3D printing tissue engineering tubular scaffold.

The utility model belongs to the technical field of cell mechanics biology experimental devices for health and rehabilitation engineering, and relates to a microfluidic device for endothelialization evaluation of a tissue engineering valve, which comprises a microfluidic chip, peripheral equipment, a signal acquisition and processing system and a feedback system. According to the utility model, the biomechanical principle and the micro-fluidic chip technology are ingeniously combined, the control system, the mechanical loading device and the micro-fluidic chip are connected to construct a micro-fluidic endothelial cell culture cavity and a peripheral system, and the real-time accurate control of the blood flow of the aortic valve is realized through the PID control system; shear stress applied to a tissue engineering valve endothelial cell culture area is generated, so that the real stress condition of the tissue engineering valve endothelial cells is simulated, and the method is used for analyzing and researching the influence rule of mechanical loading on the tissue engineering valve endothelial cells.

Disclosed are tissue engineered graft materials useful as scaffolds for blood vessel grafts in various surgical procedures and methods of making such scaffolds.

The application discloses a small-bore tissue engineering blood vessel and a preparation method thereof. The preparation method of the small-bore tissue engineering blood vessel comprises the following steps: placing an electrostatic spinning polyethylene glycol tubular film in a columnar sandwich mold layer; mixing seed cells and a first solution to prepare a seed cell-protein mixed solution, and injecting the seed cell-protein mixed solution into the columnar sandwich mold layer containing the polyethylene glycol tubular film to form a composite gel; after the seed cell-protein-polymer composite gel is statically cultured to be stable, the seed cell-protein-polymer composite gel is continuously dynamically perfusion cultured under a simulated body arterial pulsatile flow pressure condition to obtain a tissue engineering blood vessel composed of seed cells and extracellular matrix; and the tissue engineering blood vessel is subjected to a decellularization method to remove cell components in a tube wall, so that a tissue engineering blood vessel composed of only extracellular matrix is obtained.

The invention provides a magnesium oxide-loaded asymmetric short fiber aerogel scaffold as well as a preparation method and application thereof, and belongs to the technical field of tissue engineering materials. The preparation method comprises the following steps: adding MgO nanoparticles into a PLLA / Gelatin solution, and carrying out electrostatic spinning, crushing, freeze drying and crosslinking to obtain the porous short fiber aerogel scaffold (GP-coated MgO). The GP-coated MgO aerogel stent has a three-dimensional short fiber structure, and can release loaded MgO nanoparticles in a progressive and continuous manner while wound exudate is slowly absorbed. In-vivo and in-vitro experiments prove that the GP-coated MgO aerogel scaffold has biocompatibility and is very effective in the aspects of inhibiting inflammatory response and slowing down cell senescence. In addition, the GP-coated MgO significantly accelerates vascular reconstruction and epithelization, and promotes wound repair at the skin defect position of a diabetic rat.

The invention relates to a preparation method of a multi-response programmable self-disintegration structural body, a structural body formed by an outer liquid absorption layer and an inner temperature-sensitive response layer activates a response structural layer when local liquid exudes or the temperature rises, structural disintegration and sequential release of functional components are induced, the disintegration time range of the structural body is 48-72 hours, and the structural body is not prone to disintegration. The response process can be adjusted according to an actual application scene; the structural body prepared by the method is relatively high in biological safety, the inner-layer drug release is accurate and controllable, the outer-layer protects the wound surface and provides a micro-wet environment, and the structural body has temperature-sensitive intelligent responsiveness, adapts to the temperature change of the wound surface and can be applied to the wound surface. The preparation method is suitable for the following biomedical scenes: chronic wound dressings (diabetic foot and bedsore), postoperative sustained-release films, tissue engineering scaffold materials, tumor local controlled-release film carriers and the like.

The present application belongs to the technical field of hydrogel, and particularly relates to a hydrogel material with active oxygen responsiveness and a preparation method thereof, and further relates to an antibacterial / anti-ferric death bone repair composite scaffold comprising a tissue engineering porous scaffold material and the hydrogel material with active oxygen responsiveness filled in the tissue engineering porous scaffold material, in particular to an antibacterial / anti-ferric death bone repair composite scaffold comprising a 3D-printed polycaprolactone / bioglass porous scaffold material and the hydrogel material with active oxygen responsiveness filled in the porous scaffold material and a preparation method thereof, and further relates to application of the antibacterial / anti-ferric death bone repair composite scaffold with active oxygen responsiveness as described above to treatment of infectious bone defects.

The application relates to a biological 3D printed tissue engineering trachea and a construction method and application thereof, namely a cartilage / vascularized fiber tissue alternating tissue engineering trachea. The construction method comprises the following steps: firstly, cartilage specific biological ink is prepared by wrapping cartilage cells with cartilage tissue specific hydrogel; fiber specific biological ink is prepared by wrapping fibroblasts with fiber tissue specific hydrogel; then, through a double-needle extrusion type biological 3D printing technology, alternating printing is realized to form a ring-shaped tubular structure, and a cartilage / fiber tissue alternating tubular structure is constructed through multi-layer accumulation molding; further, vascularization of the fiber tissue is realized through a certain culture mode, and finally, a relatively mature cartilage / vascularized fiber tissue alternating tissue engineering trachea is obtained. The application also provides application of the cartilage / vascularized fiber tissue alternating tissue engineering trachea in tracheal defect repair.

The invention belongs to the technical field of artificial intravascular stents, and particularly relates to a small-caliber tissue engineering intravascular stent and a preparation method and application thereof. The method comprises the following steps: 1) doubling and knitting silk yarns and polylactic acid fiber yarns by using a knitting machine to obtain a tubular fabric; 2) performing heat setting to obtain an intravascular stent skeleton; (3) putting the intravascular stent skeleton into a mold filled with the gel liquid, and freeze-drying the intravascular stent skeleton; (4) crosslinking; and (5) purifying the cross-linked material to obtain the small-caliber tissue engineering intravascular stent with the inner diameter of 2-6mm. According to the invention, fibers with different degradation speeds are adopted to weave the vascular skeleton, polylactic acid is high in degradation speed, and rapid degradation is used for providing a growth space for cells; the silk is low in degradation speed and plays a role in supporting and enhancing; meanwhile, the chitosan and the gelatin hydrogel play a role in promoting cell adhesion in a scaffold texturing process.

The invention discloses a culture method of a bone tissue organ, and belongs to the technical field of tissue engineering and regenerative medicine. According to the method, a spleen is used as a tissue engineering scaffold platform with complete blood circulation and an original microenvironment, the spleen is shifted to a subcutaneous position which is easy to operate, and a bone tissue donor (including small-particle-size tissue particles or large-volume bone end segments) with development potential is implanted into spleen parenchyma; the functions of blood supply, nutrition delivery and waste removal of the spleen are utilized to realize in-vivo macroscopic development and structural reconstruction of bone tissue organs. According to the method, the natural development process of bone tissues can be simulated, long-term survival and functional maturation of organoids are supported, and an efficient and reliable experimental platform is provided for drug screening, disease modeling, developmental biology research and transplanted donor preparation.

The invention relates to a tissue engineering nerve conduit and a preparation method and application thereof. The tissue engineering nerve conduit comprises polyhydroxyalkanoate porous microspheres and a conduit, the polyhydroxyalkanoate porous microspheres are loaded in the conduit, and the conduit is made of a polyhydroxyalkanoate fibrous membrane. The porous microspheres and the fiber membrane are combined to form the composite nerve conduit, the composite nerve conduit can simulate the structure and function of autologous nerves, an ideal microenvironment is provided for growth and regeneration of nerve cells, the nerve conduit has the capacity of promoting growth and regeneration of the nerve cells, and the preparation method is simple, easy to operate and suitable for industrial production. Wide application prospects are realized in the fields of tissue engineering, neural restoration and the like.

The invention provides a pre-vascularized artificial flap and a preparation method thereof, and belongs to the field of tissue engineering materials.The preparation method comprises the steps that matrix bio-ink is printed on a substrate, and a first matrix layer in a pre-cured state is formed; printing fiber-reinforced ink on the first substrate layer in the pre-cured state to form a fiber-reinforced layer; before the fiber reinforced layer is completely cured, blood vessel bio-ink is printed on the fiber reinforced layer to form a blood vessel layer, and partial curing treatment is conducted on the blood vessel layer in the process of printing the blood vessel layer or after printing of the blood vessel layer; printing matrix bio-ink on the partially cured blood vessel layer to form a second matrix layer in a pre-cured state; the first matrix layer, the fiber reinforcement layer, the blood vessel layer and the second matrix layer are subjected to overall curing treatment, and the pre-vascularized artificial flap is obtained. According to the invention, a three-dimensional active tissue with a stable structure and a communicated vascular network can be formed, so that the interlayer stripping risk is reduced.

A tissue engineering scaffold assembly comprises a plurality of corresponding tissue engineering scaffolds. Each scaffold comprises an inner portion comprising a first set of one or more walls defining a channel extending along an axial direction from a first end to a second end of the scaffold and an outer portion comprising a second set of one or more walls substantially surrounding the first set of walls with a spacing between them defining a cavity. Each scaffold also includes a first axial interlocking part arranged at the first end of the scaffold, extending in the axial direction beyond the first and / or second set of one or more walls and a second axial interlocking part arranged at the second end of the scaffold. The second axial interlocking part of one of the plurality of corresponding tissue engineering scaffolds is engaged with the first axial interlocking part of another of the plurality of corresponding tissue engineering scaffolds to create an interlock in the axial direction.

The invention belongs to the technical field of biomedical materials and tissue engineering, and discloses a decellularized extracellular matrix complex of in-vitro tissue of a human-derived cell combined polymer scaffold and application of the decellularized extracellular matrix complex in a tissue engineering valve. The decellularized extracellular matrix complex of the in-vitro tissue of the human cells combined with the polymer scaffold comprises a sheet-shaped wall-shaped structure polymer scaffold and a human decellularized extracellular matrix attached to the scaffold. According to the invention, the extracellular matrix secreted by human cells is combined with the sheet-shaped wall-shaped structure polymer fiber scaffold, and the scaffold is fully paved with the cells by using transverse dense fibers and longitudinal sparse and staggered fibers in the scaffold, so that the extracellular matrix secreted by the human cells can be effectively compounded; the obtained decellularized extracellular matrix complex of the in-vitro tissue of the human cells combined with the polymer scaffold can effectively overcome the problems of immune rejection reaction and long-term calcification, and is particularly suitable for being applied to tissue engineering valves as bionic heart valves.

A kind of tissue engineering trachea, using additive manufacturing technology and biomaterials, bionics natural trachea morphology and function, design has cartilage-fiber alternative ring structure and bionic fiber belt structure, at the same time, design unique blood supply channel in the fiber section. Tracheal fiber section is composed of hydrogel with hierarchical porous structure, the gel is designed with circumferential blood vessel channel and radial blood vessel hole, aiming at promoting the growth of new blood vessels and growth, optimizing the process of blood supply reconstruction. Tracheal cartilage section is made into C-shaped cartilage ring with chondrocytes by bioprinting technology, providing necessary mechanical support. The fiber section and the cartilage section are effectively integrated through the chemical bond of functional groups, ensuring the stable connection of the two. Through the biological 3D printing technology, the partition construction of fiber and cartilage is realized, and the bionic tissue engineering trachea is formed through the "modular construction-integrated assembly" method, which can realize the repair and reconstruction of multi-type tissue bionic trachea, providing an innovative solution for tracheal injury.

The utility model discloses a novel improved high voltage electric field spray preparation homogeneous tissue engineering micro unit's equipment relates to the field of tissue engineering microcarrier manufacturing in biomedical engineering, including rotary needle cylinder, push rod and rotary stirring device, and the first end of push rod is provided with the piston, and the piston enters rotary needle cylinder from the big opening end of rotary needle cylinder, and the side of piston and the inner wall dynamic sealing contact of rotary needle cylinder, and the piston between the opening end of rotary needle cylinder is provided with sealing bearing, and the outer ring of sealing bearing contacts the inner wall of rotary needle cylinder, and the inner ring of sealing bearing contacts push rod stem, and rotary stirring device sets up on rotary needle cylinder. The utility model provides a novel improved high voltage electric field spray preparation homogeneous tissue engineering micro unit's equipment, and the solution of uniform solute distribution is obtained through the mode of stirring, when the microsphere spray is prepared in high pressure electrostatic, and the droplet composition of injection into high voltage electric field is high in consistency, and the composition between the generated tissue engineering micro unit is basically without difference.