Three-dimensional fiber-based aerogel tissue engineering scaffold and preparation method thereof

Abstract

The invention relates to a preparation method of a three-dimensional fiber-based aerogel tissue engineering scaffold and a product thereof. The preparation method comprises the following steps of: firstly dispersing fibers in solvents to form turbid liquid; secondly curing the turbid liquid to form cured pieces; thirdly removing cured solvents to form non-crosslinked fiber-based aerogel; finally carrying out crosslinking stabilization treatment and then carrying out sterilization treatment, thus obtaining the three-dimensional fiber-based aerogel tissue engineering scaffold. The product is a three-dimensional network-shaped material formed through mutual penetration and stagger of fibers. The fiber crossing points are effectively interconnected through non-hydrogen-bond bonding. The three-dimensional fiber-based aerogel tissue engineering scaffold has volume density of 0.1-500mg / cm<3>, average pore size of 0.01-2000mu m and specific surface area of 0.2-2000m<2> / g. The preparation method and the product have the advantages that the preparation process is simple; the raw material limitations are less; the aerogel tissue engineering scaffold product has good flexibility, connectivity and tissue growing environment and has broad application prospects in the tissue engineering field.

Description

technical field [0001] The invention relates to a three-dimensional fiber-based airgel tissue engineering scaffold and a preparation method thereof, in particular to a three-dimensional fiber-based airgel tissue engineering scaffold obtained from one-dimensional fibers through a three-dimensional network reconstruction method and a preparation method thereof. Background technique [0002] Tissue engineering is a comprehensive subject field. Its research purpose is to break through some limitations of traditional organ transplantation and biomaterial transplantation, and to develop artificial organs and tissue substitutes to replace or repair damaged organs or tissues without Adjuvant therapy, which can not only improve the cure rate, but also greatly reduce the cost of treatment. The basic principle of tissue engineering is to enable related cells to undergo a series of physiological activities such as growth, migration, and proliferation in vitro or in vivo, and finally for...

AI Technical Summary

Problems solved by technology

However, in the above-mentioned preparation processes, a large amount of polymer solution needs to be used as an adhesive component, and the prepared material is essentially a fiber-reinforced polymer porous material, which makes it difficult for the material to reflect the advantages and functional characteristics of the fiber-structured scaffold; And the use of a large amount of polymer solution can easily lead to pore blockage, and the pore size is difficult to control, which is not conducive to the growth of cells

Japanese patent P2006-75235A discloses a preparation method of a cellulose acetate fiber / polylactic acid composite three-dimensional fiber scaffold material, wherein the polylactic acid adhesive material forms a composite nano-airgel with the fiber in the form of spraying, but the material structure is a An airgel material composed of polylactic acid particles aggregated, the fiber structure characteristics of the material are difficult to reflect; and the spraying method is difficult to realize the uniform composite of polylactic acid particles and fibers, and the pore structure of the material is difficult to control

Domestic patent CN1895684 discloses a method for preparing a three-dimensional fiber scaffold material by combining natural biomaterial dissociated fibers and polymer solution. This material still has the above-mentioned structural defects, and the fiber diameter and aspect ratio obtained by dissociation of natural biomaterials deviate. Larger, making it difficult to control the pore structure of the final scaffold

Therefore, the methods disclosed in the above patents are difficult to realize the controllable preparation of three-dimensional fiber-based airgel tissue engineering scaffolds.