Three-dimensional fiber-based aerogel tissue engineering scaffold and preparation method thereof

A tissue engineering scaffold and fiber-based technology, which is applied in the field of three-dimensional fiber-based airgel tissue engineering scaffold and its preparation, can solve the problem that it is difficult to realize the controllable preparation of three-dimensional fiber-based airgel tissue engineering scaffold, and it is difficult to reflect the fiber structure scaffold Advantages and functional characteristics, it is difficult to realize uniform compounding of polylactic acid particles and fibers, etc., to achieve good structural controllability, broaden the scope of application, and high media transmission efficiency

Active Publication Date: 2013-09-11
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the above-mentioned preparation processes, a large amount of polymer solution needs to be used as an adhesive component, and the prepared material is essentially a fiber-reinforced polymer porous material, which makes it difficult for the material to reflect the advantages and functional characteristics of the fiber-structured scaffold; And the use of a large amount of polymer solution can easily lead to pore blockage, and the pore size is difficult to control, which is not conducive to the growth of cells
Japanese patent P2006-75235A discloses a preparation method of a cellulose acetate fiber/polylactic acid composite three-dimensional fiber scaffold material, wherein the polylactic acid adhesive material forms a composite nano-airgel with the fiber in the form of spraying, but the material structure is a An airgel material composed of polylactic

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A method for preparing a three-dimensional fiber-based airgel tissue engineering scaffold, the specific steps are:

[0036] The first step: dispersing cotton fibers with an average diameter of 15 μm and an aspect ratio of 500 in water to form a homogeneous suspension; in the suspension, the mass fraction of fibers is 1%;

[0037] Step 2: aging the suspension at 25°C for 6 hours to make the suspension form a coagulated block;

[0038] The third step: using supercritical drying and infrared drying methods to remove the solidified water in the solidified block to form uncrosslinked fiber-based airgel;

[0039] Step 4: Thermally cross-link the uncrosslinked fiber-based aerogel for 2 hours to obtain preliminary cross-linking, and then use ultrasonic cross-linking for 0.5 h to obtain a three-dimensional fiber-based aerogel bonded and fixed at interlaced points of fibers Material;

[0040] The fifth step: the cross-linked three-dimensional fiber-based airgel material is subj...

Embodiment 2

[0042] A method for preparing a three-dimensional fiber-based airgel tissue engineering scaffold, the specific steps are:

[0043] The first step: the silk fiber with an average diameter of 15 μm and an aspect ratio of 50, the polylactic acid-caprolactone fiber with an average diameter of 10 μm and an aspect ratio of 100, and the hydroxyl fiber with an average diameter of 6 μm and an aspect ratio of 500 Propyl cellulose fibers, an average diameter of 12 μm, a dextran fiber with an aspect ratio of 90, an average diameter of 10 μm, and a polyamide fiber with an aspect ratio of 100 are dispersed in tert-butanol to form a homogeneous suspension; In the suspension, the massfraction of fiber is 12%;

[0044] Step 2: aging the suspension at 25°C for 6 hours to make the suspension form a coagulated block;

[0045] The third step: using supercritical drying and infrared drying methods to remove the solidified tert-butanol in the solidified block to form uncrosslinked fiber-based airge...

Embodiment 3

[0049] A method for preparing a three-dimensional fiber-based airgel tissue engineering scaffold, the specific steps are:

[0050] The first step: hemp fibers with an average diameter of 20 μm and an aspect ratio of 5, cellulose acetate fibers with an average diameter of 600 nm and an aspect ratio of 2000, copper ammonium fibers with an average diameter of 2 μm and an aspect ratio of 600, The average diameter is 3 μm, the polytrihydroxybutyrate fiber that the aspect ratio is 600, the average diameter is 300nm, and the zirconium dioxide fiber that the aspect ratio is 5000 is dispersed in water to form a homogeneous suspension; the suspension In, the mass fraction of fiber is 5%;

[0051] Step 2: freeze the suspension at -30°C for 8 hours to make the suspension form a coagulated block;

[0052] The third step: using freeze drying, vacuum drying, and infrared drying to remove the water solidified in the solidified block to form an uncrosslinked fiber-based airgel;

[0053] Step...

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Abstract

The invention relates to a preparation method of a three-dimensional fiber-based aerogel tissue engineering scaffold and a product thereof. The preparation method comprises the following steps of: firstly dispersing fibers in solvents to form turbid liquid; secondly curing the turbid liquid to form cured pieces; thirdly removing cured solvents to form non-crosslinked fiber-based aerogel; finally carrying out crosslinking stabilization treatment and then carrying out sterilization treatment, thus obtaining the three-dimensional fiber-based aerogel tissue engineering scaffold. The product is a three-dimensional network-shaped material formed through mutual penetration and stagger of fibers. The fiber crossing points are effectively interconnected through non-hydrogen-bond bonding. The three-dimensional fiber-based aerogel tissue engineering scaffold has volume density of 0.1-500mg/cm<3>, average pore size of 0.01-2000mu m and specific surface area of 0.2-2000m<2>/g. The preparation method and the product have the advantages that the preparation process is simple; the raw material limitations are less; the aerogel tissue engineering scaffold product has good flexibility, connectivity and tissue growing environment and has broad application prospects in the tissue engineering field.

Description

technical field [0001] The invention relates to a three-dimensional fiber-based airgel tissue engineering scaffold and a preparation method thereof, in particular to a three-dimensional fiber-based airgel tissue engineering scaffold obtained from one-dimensional fibers through a three-dimensional network reconstruction method and a preparation method thereof. Background technique [0002] Tissue engineering is a comprehensive subject field. Its research purpose is to break through some limitations of traditional organ transplantation and biomaterial transplantation, and to develop artificial organs and tissue substitutes to replace or repair damaged organs or tissues without Adjuvant therapy, which can not only improve the cure rate, but also greatly reduce the cost of treatment. The basic principle of tissue engineering is to enable related cells to undergo a series of physiological activities such as growth, migration, and proliferation in vitro or in vivo, and finally for...

Claims

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Application Information

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IPC IPC(8): A61L27/26A61L27/20A61L27/22A61L27/04A61L27/08A61L27/18
Inventor 丁彬斯阳葛建龙唐晓敏黄美玲朱婕俞建勇
Owner DONGHUA UNIV
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