The invention relates to a piece of glass with low resistance and low optical loss and a preparation method thereof. A multilayer thin film structure is prepared on inorganic glass, polycarbonate or organic glass by utilizing a coating technology and a magnetron sputtering technology and comprises a transition layer, a dielectric layer, a protective layer, a conductive layer, a protective layer, an anti-reflection layer and a wear-resistant layer, so that the multilayer thin film structure has low resistance and low optical loss performance. Compared with a current common indium tin oxide/silver/indium tin oxide sandwich structure, the multilayer thin film structure has the advantages of being low in cost, non-toxic, good in mechanical flexibility, good in chemical stability, good in filmadhesion, low in resistance, good in light transmission performance, suitable for high-temperature-resistant base materials and the like.