PCT No. PCT/EP96/04377 Sec. 371 Date May 11, 1998 Sec. 102(e) Date May 11, 1998 PCT Filed Oct. 8, 1996 PCT Pub. No. WO97/13810 PCT Pub. Date Apr. 17, 1997The present invention is directed to fluorescent dyes and its valence tautomers of the formula wherein Q represents a conjugated moiety that increases the fluorescent quantum yield of the compound; R1 is a functionalized group of the formula-(CH2)jY, wherein Y is selected from the group consisting of SO3H, COOH, NH2, CHO, NCS, epoxy, phthalimido, and COOZ, wherein Z represents a leaving group; R2 is a functionalized group of the formula -(CH2)kY', wherein Y' is selected from the group consisting of SO3H, COOH, NH2, CHO, NCS, epoxy, phthalimido, and COOZ, wherein Z represents a leaving group; M+ is a counterion selected from the group consisting of ammonium, alkali metal cations, and alkaline earth metal cations; n=1 to 4; m=1 to 4; j=2 to 10; and k=2 to 10.