The invention belongs to the field of chemical coatings, and particularly relates to a graphene nano-silver antibacterial coating and a preparation method thereof. The graphene nano-silver antibacterial coating comprises water, cellulose, a wetting agent, an antifreezing agent, a thickening agent, a defoaming agent, titanium dioxide slurry, filler slurry, a multifunctional amine auxiliary, emulsion, a sterilizing agent, a coalescing agent, a flatting agent, a formaldehyde-resistant auxiliary, and a silver-bearing antibacterial agent, and the silver-bearing antibacterial agent is graphene nano-silver dispersion liquid. According to the graphene nano-silver antibacterial coating and the preparation method thereof, a traditional formula is improved, the specially-made graphene nano-silver dispersion liquid is added to the formula, and the antibacterial effect is achieved. Nano silver can further destroy a microorganism electron transmission system, a respiratory system and a material transfer system. When the activity of thalli is lost, the nano silver can be dissociated out of the thalli and repeatedly conducts sterilization activities, and therefore the long-acting antibacterial function of the coating can be maintained. The nano silver can exert the lasting antibacterial effect by slowly releasing Ag<+>.