23096 results about "Microorganism" patented technology

The invention relates to ice wine yeast with high fermentation activity and outstanding aroma-producing characteristics and application thereof. The ice wine yeast is yeast (Saccharomyces cerevisiae) YSL16010, and the bacterial strain has been preserved in China General Microbiological Culture Collection Center on April 18, 2016, with a preservation number of CGMCC No.12349. The bacterial strain YSL16010 is obtained by separating and screening Vidal grapes from a Huanren producing area of Liaoning at a temperature of 16 DEG C in a natural fermentation process, and the ice wine wine-making yeast having good making characteristics and highly producing esters is obtained through a series of screening and identifications and small pilot scale fermentation. The ice wine wine-making yeast YSL1610 highly producing esters is used for fermenting and producing ice wine, so that the yield of volatile acid is effectively controlled while the standards of the grape wine producing countries can be met, and therefore, the content of aromatic compounds such as esters is remarkably increased. The bacterial strain can be applied to ice wine brewing industry, and can play an important role in improving the quality of the ice wine and bringing advantages of the grape producing areas into play.

The invention provides bacillus and application thereof, the bacillus is preserved in Guangdong Microbial Culture Collection Center on June 6, 2025, and the preservation number is GDMCC No.66483; one application of the bacillus is to prepare a preparation for promoting growth and / or resisting diseases of plants, and the other application is to use the bacillus for inhibiting microorganisms; the bacillus can effectively and enduringly inhibit the growth of phytopathogen, and can be used as a broad-spectrum antibacterial biological antibacterial agent to enhance the resistance of plants and promote the growth of the plants.

The invention relates to the technical field of microbial fermentation control, in particular to an automatic microbial reaction fermentation control system and a control method. The method comprises the following steps: collecting real-time microbial environment parameters and identifying a data multi-axis structure, extracting master control environment interval data, eliminating redundant signals to generate standard microbial environment data, defining a fermentation degree contrast template through fermentation historical records, and constructing a microbial fermentation timeline framework. The method comprises the following steps: acquiring standard environmental data, identifying a fermentation state of the standard environmental data, constructing a simulated microbial environmental field by using the standard microbial environmental data, performing global microbial reaction simulation, determining fermentation reaction structure partitions based on the data, performing difference comparison to generate fermentation difference data, and performing compensation simulation by using the difference data. And automatic fermentation compensation control of microorganisms is realized. According to the invention, the intelligent automatic control and optimization of the microbial fermentation process are realized, the fermentation efficiency and stability are obviously improved, and the manual intervention is reduced.

The invention discloses coal gangue-based artificial ecological soil and a preparation method and application thereof, and belongs to the technical field of ecological improvement, the coal gangue-based artificial ecological soil comprises crushed coal gangue and a compound microbial agent, and the compound microbial agent comprises a microbial activating agent and a microbial passivating agent. According to the invention, the coal gangue can be quickly decomposed through the microbial activating agent, useful mineral elements in the coal gangue can be fully activated and analyzed, and the effect of activating a nutrient library in the coal gangue is achieved, so that useful trace elements contained in the coal gangue are effectively released and are easily absorbed by plant growth; meanwhile, harmful heavy metal elements in the coal gangue are passivated through the microbial passivating fungicide, the water-retaining property and the air permeability of the soil are guaranteed by controlling the decomposition particle size proportion of the coal gangue, the soil conditioner can be used for ecological restoration, desert control, agriculture and animal husbandry planting, flower and nursery planting and urban landscaping, the effects of improving the soil and promoting plant growth are achieved, and the soil conditioner is suitable for large-scale popularization and application. The application is wide.

The invention discloses an illness state tracking management system and method based on a lingual face diagnosis instrument, and belongs to the technical field of traditional Chinese medicine tongue diagnosis and modern information technology fusion. The system comprises a multi-modal data acquisition module, a data processing and analysis module and an augmented reality visualization module; according to the method, illness state tracking is achieved through the steps of multi-modal data acquisition, data preprocessing and feature fusion, personalized digital twinborn model establishment, augmented reality visualization presentation and the like, multi-dimensional data such as tongue picture macroscopic features and tongue surface microorganism distribution can be integrated, dynamic association between the data is revealed, the health state and the intervention effect are visually displayed, and the method is suitable for being popularized and applied. The method is suitable for the fields of traditional Chinese medicine health management and chronic disease monitoring.

The invention relates to the technical field of data processing. The fermentation process intelligent regulation and control method and system based on multi-modal perception are provided, and the method comprises the following steps: carrying out image feature extraction processing on microorganism image data to generate a morphological feature vector, and carrying out metabolic feature dimension reduction processing on metabonomics data to generate a metabolic feature matrix; performing time sequence alignment processing to generate a fusion feature matrix, and performing abnormal marking processing on the metabonomics data to generate abnormal marking data; carrying out correlation intensity calculation processing on the morphological change of the microorganisms and the concentration fluctuation of the metabolites to generate a dynamic correlation intensity curve; constructing a cross-dimensional anomaly recognition model and a multi-modal collaborative prediction model, and generating a regulation and control parameter suggested value; the parameters of the multi-modal collaborative prediction model are updated through a feedback learning mechanism, the feature fusion weight of the fusion feature matrix is optimized, the accuracy of anomaly detection and regulation decision is improved, and the risk of stability fluctuation in the fermentation process is reduced.

The invention relates to the field of microbial communities, and discloses a bioinformatics-based microbial community dynamic monitoring method, which comprises the following steps: acquiring high-frequency acquisition data based on a trace sample, constructing a microbial data stream for time sequence analysis, and carrying out rapid metagenome marker amplification on each sampling unit in the data stream, obtaining a preliminary feature matrix; based on the co-occurrence frequency of the microbial functional genes in the preliminary feature matrix, constructing a multi-dimensional feature mapping graph; performing real-time mode recognition on the flora abundance change trend based on the dynamic fluctuation region; aiming at the key dynamic signal segment, adopting a distributed clustering method based on variation information entropy regulation and control to reconstruct the evolution trajectory of the flora, and generating a flora time sequence behavior vector set; and based on the flora time sequence behavior vector set, performing dynamic alignment with a pre-constructed reference model by using a multi-scale trend matching algorithm. The method has the advantages of high timeliness and automatic processing capability.

The invention discloses a growth-promoting bacillus velezensis FSUXF-717 capable of tolerating heavy metals and application of the growth-promoting bacillus velezensis FSUXF-717, and belongs to the technical field of agricultural microorganisms. The bacillus velezensis FSUXF-717 has the following advantages: (1) the bacillus velezensis FSUXF-717 has multifunctional metabolic characteristics: various carbon sources such as cane sugar, raffinose and aesculin can be utilized, and the bacillus velezensis FSUXF-717 has the functions of producing long-chain hormone, dissolving phosphorus, secreting ACC deaminase, efficiently forming a biological membrane and the like; (2) broad-spectrum adaptability: the strain has strong tolerance to heavy metal pollution (Cd / Pb / Cr / Ni), drought and broad-spectrum acid-base stress (pH 4.5-9.5); under the condition of relatively low bacterium concentration, the auxin can be stably synthesized in a heavy metal-acid-base composite stress environment. And (3) the activity of soil sucrase and soil phosphatase is enhanced. Excavation of the strain can provide an important germplasm resource basis for development and utilization of novel efficient microbial fertilizers and lay a foundation for green and high-quality development of agriculture.

The invention belongs to the technical field of microorganisms, particularly relates to rice endophytic bacillus velezensis AN6 and application thereof, and particularly provides bacillus velezensis AN6 which is classified and named as bacillus velezensis and has the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 20231886. The invention provides a novel rice endophytic bacillus velezensis strain AN6, through separation, screening, identification and experimental verification, it is found that the strain shows broad-spectrum resistance to various phytopathogens including rice bacterial leaf blight, rice blast and the like, the strain has the capacity of inhibiting various phytopathogens and has the capacity of adsorbing heavy metal, and the strain has the advantages of being capable of inhibiting various phytopathogens and capable of being used as a novel rice endophytic bacillus velezensis strain AN6. The method is of great significance to biological control of rice diseases and development of green agriculture.

The invention relates to the technical field of river and lake landscape water system purification, and discloses a river and lake landscape water system composite purification system, which comprises a water quality parameter acquisition module, an ecological analysis module, a microbiological treatment module, a multi-stage collaborative purification module, a purification parameter generation module, an authority association module and a dynamic regulation and control module. The water quality parameter acquisition module acquires data in multiple dimensions to quantify the pollution level and trigger a purification instruction; the ecological analysis module evaluates the ecological self-purification capability, and the microbiological treatment module predicts the degradation efficiency; the multi-stage collaborative purification module generates a regulation and control signal in combination with the evaluation value; the purification parameter generation module generates repair and activation parameters accordingly; the permission association module monitors the emission permission of the pollution source; and the dynamic regulation and control module forms a closed-loop purification process. According to the system, through multi-module cooperation and closed-loop regulation and control, accurate recognition of river and lake landscape water system pollution, ecological self-purification capability activation and microbial degradation enhancement are achieved, and the purification efficiency and ecological restoration long-term effect are improved.

The invention provides a Pseudomonas protegens P150 strain and an application of the Pseudomonas protegens P150 strain, and belongs to the technical field of biocontrol bacteria. The defensive pseudomonas P150 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.34520, the preservation time is May 13, 2025, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The defensive pseudomonas P150 disclosed by the invention has the effects of inhibiting various pathogenic bacteria, producing enzymes, promoting growth and preventing and treating tomato bacterial wilt and soybean pustule. The microbial inoculum prepared by adopting the biocontrol bacterium can reduce the dosage of chemical pesticides and the prevention and control cost of plant diseases, is green and pollution-free, can be used for preventing and controlling tomato bacterial wilt and soybean pustule, and has a relatively good popularization value.

The invention discloses a system and method for identifying microbial contamination in food and cosmetics based on AI identification, and the method comprises the steps: collecting to-be-detected food or cosmetics as a detection sample, and obtaining the bacterial colony growth state of microorganisms in the detection sample; the method comprises the following steps: acquiring a microbial growth image through microscopic imaging equipment, and preprocessing the microbial growth image to obtain a target area of a microbial colony; constructing an AI recognition model by adopting a convolutional neural network, and obtaining the species and quantity of microorganisms in the detection sample; parameters of the AI recognition model are adjusted in real time according to the types and the number of the microorganisms, a preset microorganism limited standard threshold value is combined, a visual analysis report is generated, the AI recognition model is combined with a Faster R-CNN target detection method, precise classification and number statistics of the types of the microorganisms are achieved, a complete microorganism pollution analysis report is formed, and the method is suitable for popularization and application. And the accuracy and scientificity of the final identification result are improved.

The invention discloses a method for detecting probiotics in fermented rotten vegetable feed based on the Internet of Things, and relates to the technical field of microbiological detection, and the method comprises the following steps: obtaining complete echo signal time sequence data, and carrying out high-frequency sampling on an echo signal time sequence by a sliding time window with a fixed length, and constructing a three-dimensional optical signal transient feature vector. According to the method, the transient feature vector of the three-dimensional optical signal is constructed, and a disturbance residual error and time sensitive weight mechanism is introduced, so that the abnormal disturbance of the optical signal is accurately recognized; by combining the optical interference index and the dynamic sampling window adjustment strategy, the refractive index mutation area is automatically avoided, the sensing misjudgment and data distortion are avoided, the detection stability and accuracy of the probiotic activity state in the fermentation environment are remarkably improved, and the intelligent monitoring capability of the feed quality is enhanced.

The invention belongs to the technical field of soil fertilizers, and particularly relates to a soil conditioner and a preparation method thereof. Wherein the soil conditioner is prepared from the following raw materials in parts by weight: 30 to 40 parts of modified charcoal, 20 to 30 parts of microbial complexing agent, 15 to 25 parts of humic acid and 1 to 5 parts of biological enzyme; according to the microbial complexing agent, dopamine grafted and modified nano silicon dioxide and hydroxyapatite form a composite shell, pseudomonas aeruginosa and bacillus subtilis are wrapped, and the microbial complexing agent has the functions of protecting microorganisms, adsorbing heavy metals and slowly releasing phosphorus elements; the modified biochar is compounded with the attapulgite through acid-base treatment to form a porous structure with physical inlaying and chemical functional groups synergistic, so that the adsorption and fixation capability on heavy metals and organic pollutants is remarkably improved; the biological enzyme accelerates organic phosphorus conversion, and enzymatic hydrolysate provides a phosphorus source for microorganisms, promotes microorganism breeding, effectively increases the content of available phosphorus in soil, and enhances the stability of a remediation system.

The invention relates to the technical field of sewage treatment, in particular to a coupling control system and method for deep denitrification of sewage, and the system comprises a real-time water quality monitoring module, a microorganism twinborn modeling module, a real-time prediction module, an intelligent decision module, an execution mechanism module and a prediction regulation and control module. Compared with the prior art that a passive feedback control strategy based on an effluent quality index is generally adopted, the hysteresis quality is high, and violent fluctuation of an inflow load cannot be coped with; according to the method, a digital twinborn body capable of reflecting the functional state of a microbial community in real time is constructed, and a control target is improved from a traditional process parameter set point to direct optimization of microbial ecological functions; according to the invention, the method achieves the fundamental crossing from the control of technological parameters to the regulation and control of microbial ecology, can carry out intervention from the root of the reaction process, enables the system to have the active health management capability, and remarkably improves the stability of the treatment efficiency and the intelligent level of coping with complex working conditions.

Provided herein are methods, compositions, and kits for determining the spatial location of target nucleic acids, including endogenous and exogenous target nucleic acids, in a biological sample using padlock probes and substrates with spatially barcoded capture probes. Also disclosed herein are methods for determining a presence and / or location of a microbe (e.g., archaea, fungi, bacteria) in a biological sample, e.g., by determining the presence and / or location of a microbial target nucleic acid in the biological sample.

Platforms, systems, and methods for genetic generalization in synthetic biology development. According to one aspect, there is provided a method for predicting performance associated with genetic edits, the method comprising: receiving, by a platform, information about a strain of a microorganism, wherein the information about the strain comprises information describing a plurality of genetic edits to a base strain of the microorganism; generating, by the platform, a set of genetic embeddings based on the information about the strain, wherein the generating comprises processing the information about the strain using one or more embedding models, wherein each of the one or more embedding models: receives the information about the strain of the microorganism as input; and applies computational transformations to the input using a corresponding embedding model to generate a multi-dimensional vector representation for each of the plurality of genetic edits.

The present disclosure is drawn to methods of utilizing nucleic acid barcodes and corresponding amplifying sites in cells in which the barcodes naturally occur. These barcodes and amplifying sites are reconfigured into a single nucleic acid cassette that provides for ease of use in tagging particular species, strains, or variants of cells, each with a different barcode. These barcodes can be used to track the colonization capabilities of the barcoded cells. The present disclosure further provides for assays that utilize natural barcodes to measure relative microbial colonization ability of a plant root system.

The invention discloses an intelligent regulation and control method for a tea fermentation process and a regulation and control system based on microbial activity monitoring, and relates to the technical field of tea processing. According to the method, microbial activity and environmental parameters are monitored in real time, and a microbial metabolism model and a neural network algorithm are combined, so that dynamic optimization and accurate regulation and control of the tea leaf fermentation process are realized, firstly, microbial activity data and environmental parameters in a fermentation environment are collected by utilizing a sensor array; the method comprises the following steps: firstly, calculating a metabolite generation rate and a concentration change trend through a microbial metabolism model, accurately identifying abnormal fluctuation in a fermentation process, secondly, adjusting fermentation conditions in real time based on the metabolite concentration change trend and a dynamic regulation and control algorithm to ensure the stability of the fermentation process, and finally, carrying out real-time fermentation. Tea quality parameters after fermentation condition adjustment are predicted through a neural network algorithm, a regulation and control strategy is optimized, and stable improvement of tea quality is achieved.

The invention relates to the field of microorganisms, in particular to a mortierella alpina strain MA with a plant growth promoting function and application of the mortierella alpina strain MA. The strain has remarkable nitrogen fixation, siderophore production, auxin (IAA) production and cellulase activity, can inhibit various soil-borne pathogenic bacteria such as phytophthora capsici and fusarium, and remarkably improves the disease resistance and growth performance of crops such as pepper. The strain has various functions and broad-spectrum action, is suitable for replacing part of chemical fertilizers and pesticides in intensive agriculture, and realizes green and efficient planting of crops.

The invention discloses a multifunctional low-temperature corn straw degradation complex microbial inoculant, a preparation method and application, the complex microbial inoculant is compounded by a plurality of microorganisms with straw degradation, potassium dissolving and growth promoting functions, and the complex microbial inoculant comprises the following strains: bacillus subtilis K15, curveolate Prickia L11 and bacillus mycoides L31. The complex microbial inoculant disclosed by the invention realizes synergistic interaction and functional complementation, and through scientific compounding of strains, the straw decomposition rate is remarkably increased, and the decomposition period is obviously shortened; the adaptive capacity and colonization competitiveness of microorganisms in a low-temperature and complex soil environment in winter are enhanced. The complex microbial inoculant disclosed by the invention also has multiple functions of degrading straws, dissolving potassium, producing IAA (Indoleacetic Acid) and the like. The soil potassium which is difficult to utilize can be converted into quick-acting potassium while the straws are quickly decomposed to release nutrients.

The invention relates to the technical field of automatic control, and discloses a water affair treatment process optimization control method, system and equipment and a medium. The method comprises the steps of performing state judgment on sensor detection data through a multi-parameter state recognition algorithm to obtain a processing stage switching instruction; modeling a microbial activity change rule according to the switching instruction to obtain a staged prediction model; optimizing the prediction model through a switching model prediction control algorithm to obtain a multi-target control strategy; performing coordinated calculation on the set value according to the control strategy to obtain a distributed control instruction; and performing driving control on the equipment by using the control instruction through the field bus to obtain a coordinated operation state. According to the invention, the control precision and the multi-target coordination optimization capability of the water affair treatment process are improved.

The invention relates to the technical field of agricultural fertilizers, discloses a seaweed extract-functional microorganism compound slow-release fertilizer and a preparation method thereof, and solves the problems in the background art. The seaweed extract is a compound containing enteromorpha polysaccharide and alginic acid extract; the functional microorganism is a complex microbial inoculant composed of phosphate solubilizing bacteria, nitrogen-fixing bacteria, phosphate solubilizing bacteria and bacillus; the slow-release carrier is prepared by coating the seaweed extract and the functional microorganisms with sodium alginate-chitosan microcapsules; the signal molecule is selected from at least one of polyamine substances and jasmonic acid methyl ester; the fertilizer has the advantages that the utilization rate of the fertilizer can be increased, growth and reproduction of soil microorganisms are promoted, the stress resistance of crops is improved, and the requirements of different soil types and planting environments can be met.

The invention relates to the technical field of biochemical culture, in particular to a biochemical incubator with multiple bins and a method thereof.The biochemical incubator comprises a case, a cavity is formed in the middle of the case, the cavity is divided into a plurality of culture bins by a plurality of partition plates, treatment cavities are formed in the two sides of the cavity, protective shells are connected to the outer sides of the treatment cavities, and storage assemblies are arranged in the culture bins; the storage assembly comprises a movable bottom plate and three groups of longitudinal partition plates; the longitudinal partition plate divides the movable bottom plate into two storage cavities, storage tables are arranged in the storage cavities, and the movable bottom plate is driven by the pushing assembly to move left and right and used for alternately feeding the two storage tables into the culture bin; two groups of sealing covers are arranged outside the storage table, and are driven to be opened or closed by a turnover assembly; according to the incubator, pollution-free conveying of materials is achieved, and meanwhile the stability of environmental parameters of all the bins is maintained; the culture method ensures that different batches of samples are cultured in an independent environment, and cross contamination between microorganism or cell samples is avoided.

The invention belongs to the technical field of microbial fermentation and food engineering, and discloses a fermentation method for enhancing the production capacity of schizochytrium limacinum phospholipid DHA and application, schizochytrium limacinum is subjected to fermentation culture for 32-60 h, and the addition of an organic nitrogen source and phosphate is optimized in the fermentation culture process; the formula of a seed culture medium for culturing schizochytrium limacinum is not changed, and only the formula of a fermentation culture medium for schizochytrium limacinum is changed. The invention provides a fermentation strategy for enhancing the production capacity of the schizochytrium limacinum phospholipid DHA, and the schizochytrium limacinum can obtain the phospholipid DHA with higher content by optimizing an organic nitrogen source and phosphate in a schizochytrium limacinum fermentation culture medium. According to the obtained schizochytrium limacinum grease, the total DHA content is obviously increased compared with that of a traditional fermentation strategy, and the proportion of phospholipid DHA is also remarkably increased. The regulation and control method is applied to preparation of schizochytrium limacinum algae powder, the schizochytrium limacinum algae powder rich in phospholipid DHA is obtained, and application of the schizochytrium limacinum algae powder in the field of aquatic feed is facilitated.

The invention provides a treatment method of ammonia-nitrogen-containing wastewater, and belongs to the technical field of wastewater treatment. The preparation process comprises the following steps: preparing the wastewater treatment agent; preparing an immobilized microbial compound; and treating the ammonia-nitrogen-containing wastewater. According to the immobilized microbial carrier prepared by the invention, a stable dual-network structure and proper pore channels are formed through the synergistic effect of the polyvinyl alcohol, the carboxymethyl chitosan and the polyurethane; secondary cross-linking significantly enhances the stability of the carrier, optimizes internal pore channels, and is beneficial to attachment and proliferation of microorganisms; after the corn straw biochar is modified by Ca / Al, the specific surface area and functional groups are increased, and the physicochemical adsorption of ammonia nitrogen is enhanced. The three are combined, and ammonia nitrogen in wastewater is efficiently removed through synergism of carrier adsorption and microbial metabolism.

The invention belongs to the technical field of water treatment, and particularly provides a fungus filament bamboo-based biomass composite material and a lead-containing sewage treatment method. According to the invention, the lead-containing sewage is pretreated by using microorganism neurospora crassa, and then the lead-containing sewage is post-treated by using the fungus filament bamboo-based biomass composite material prepared under specific conditions, so that the mildness of microorganism treatment and the efficient adsorbability of the biomass composite material are effectively combined; and finally, the removal rate of heavy metal lead is increased, the cost is low, and environmental friendliness is achieved.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to pseudomonas fluorescens as well as a microbial agent and application thereof. The invention discloses a pseudomonas fluorescens TJ194, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.33697. The pseudomonas fluorescens TJ194 has the advantages that the pseudomonas fluorescens TJ194 can be used for preparing the pseudomonas fluorescens TJ194, and the pseudomonas fluorescens TJ194 can be used for preparing the pseudomonas fluorescens TJ194; tests prove that the strain has the activity of inhibiting the growth of fusarium pseudograminearum; by applying a fungicide containing the strain, the disease index of the wheat stem rot is remarkably reduced, and meanwhile, the fresh weight of the overground part and the underground part of wheat is increased, so that the pseudomonas fluorescens TJ194 and the fungicide thereof can be applied to prevention and treatment of the plant stem rot and promotion of plant growth.

The invention discloses staphylococcus xylosus with a lipid degradation function and application of the staphylococcus xylosus in fermented food, and belongs to the field of microorganisms. The invention provides staphylococcus xylosus S3, the staphylococcus xylosus S3 has the capability of degrading lipid, and when the staphylococcus xylosus S3 is fermented in surimi for 48 hours, the acid value and the TBARS value of the fermented surimi can be effectively improved. Therefore, the staphylococcus xylosus S3 can adjust the lipid hydrolysis degree in the fermented surimi to an ideal range, and has broad application prospects in the food industry.

The invention relates to the technical field of microorganisms, in particular to application of streptococcus non-lactolyticus GX-7 and a complex microbial inoculant thereof in sugarcane leaf silage, the strain GX-7 is separated from healthy pig manure by a research group, and through detection, the strain has good cholate resistance and is suitable for surviving in intestinal tracts, and the strain GX-7 has good cholate resistance and is suitable for being used as a feed additive. The antibacterial effect on pseudomonas fluorescens, listeria monocytogenes, salmonella choleraesuis and shigella flexneri is achieved; the strain has a good fermentation capability on sugarcane leaves, and the sugarcane leaves are inoculated with a complex microbial inoculant prepared from streptococcus non-lactolyticus GX-7 and streptococcus non-lactolyticus GX-2, so that a synergistic fermentation effect can be achieved; after the silage prepared from the complex microbial inoculant is fed to female buffalo in the gestation period, it is found that the silage can effectively increase the content of milk protein, milk total solids and milk non-fat solids in colostrum of the female buffalo and relieve diarrhea of the female buffalo, and the silage is safe and good.