3 results about "Bacterial host" patented technology

DEPCT6604 / 07 / 2566 This invention involves a DNA structure suitable for expression. Recombinant proteins in bacterial host cells that include the following DNA structure: Coding nucleotide sequence: promoter rhaBAD, RhaR transcription activater, RhaS Transcription activators, antibiotic resistance indicators, actively linked promoters. Along with nucleic acids that encode indicators of antibiotic resistance, the terminators rrnBT1 and rrnBT2. The point for initiating self-replication, pMB1, is a DNA structure that may also be assembled with sequences. The nucleotides that encode the recombinant protein are functionally linked to the promoter. This invention, rhaBAD, involves, in addition to a vector and a bacterial host cell that are combined. Given this DNA structure and the method of producing recombinant proteins... By contacting the host bacterial cells with rhamnose, the expression of these cells is stimulated. Of the aforementioned recombinant proteins;

The present disclosure provides modified bacterial cell lysate compositions and methods for cell-free phage synthesis (CFBS) using the compositions to produce a wide range of phage species. Also disclosed herein are bacteriophage engineering methods for converting a lysogenic bacteriophage to a lytic bacteriophage, and methods for producing a genetically modified bacteriophage species that target a wider range of bacterial host strains than a wild type bacteriophage species.

The present invention provides recombinant gram-negative host cells that do not degrade protease-sensitive recombinant proteins yet grow to high cell density, methods for the use of these host cells to produce high-quality recombinant proteins, including antibodies and antibody fragments, at high yield, as well as compositions and methods relating to periplasmic expression of recombinant proteins or polypeptides of interest in host cells.