127 results about "Bacteriocyte" patented technology

The invention discloses an intelligent quintuplet cooperative plasma self-purification robot long-term storage system and a self-adaptive disinfection control method. According to the system, cold plasma, ozone, chlorine dioxide, self-generated nitric oxide and a charge self-purification jump effect are fused, and a dynamic self-purification system with an electric field-gas field-chemical field-intelligent field-thermal field five-dimensional synergistic energy field is constructed. The robot adopts a three-section structural layout and a multi-mode self-adaptive propulsion mechanism, environmental parameters are sensed through AI, discharge power and oxidant release are intelligently regulated and controlled, and precise sterilization, toxicity reduction and mildew prevention in a multi-medium environment are achieved. The system can generate various high-energy active species containing. OH, O, NO, ClO, ONOO and the like, chain splitting decomposition and oxidation mineralization are carried out on mold spores, bacterial cells, aflatoxin and the like, the sterilization rate reaches 99.995%, and the toxin degradation rate exceeds 98%. The system has the characteristics of no residue, low energy consumption, long-acting self-maintenance and the like, is suitable for multiple scenes of grain storage, traditional Chinese medicinal materials, cold chains, farmland, public health and the like, and realizes an intelligent, continuous and chemical-additive-free green storage and dynamic purification system.

The invention relates to the field of biological environmental protection, in particular to a heterotrophic nitrification immobilized engineering bacterium for efficient denitrification and a preparation method and application thereof.According to the heterotrophic nitrification immobilized engineering bacterium for efficient denitrification and the preparation method and application of the heterotrophic nitrification immobilized engineering bacterium, polyethylene glycol-glucan two-aqueous-phase concentration and trehalose protection liquid are adopted for synergistic effect, the bottleneck of cell damage caused by a traditional flocculation method can be avoided, trehalose molecules can wrap bacterial cells, and the bacterial cells can be effectively degraded; the immobilized particles can resist osmotic pressure impact and pollutant invasion, the survival rate of thalli is higher than 95%, so that the immobilized particles always keep ultrahigh denitrifying enzyme activity in various sewage environments, honeycomb-shaped pores are formed in an ethyl cellulose matrix, a microenvironment with a high specific surface area is provided for thalli attachment, and the built-in nano zeolite continuously and slowly releases Cu < 2 + > and Zn < 2 + > trace elements, so that the immobilized particles can be recycled. A thallus metabolism enzyme system is synergistically activated, and the denitrification pathway function is enhanced; the chitosan-polydopamine outer coating layer strongly captures the nitrogen-containing pollutants, so that the removal efficiency of the nitrogen-containing pollutants is improved; through triple protection, the density of unit granular bacteria is up to 109 cells / cm, the service life is prolonged, and the denitrification performance is improved by more than 40%.

Provided herein are methods for the production of a vector with a size of at least 16 kb from bacterial cells comprising the consecutive steps of a) obtaining bacterial cells comprising a vector with a size of at least 16 kb, comprising an inducible origin of replication, b) inoculating culture medium with the bacterial cells comprising the vector, c) culturing the bacterial cells in the culture medium, d) adding one or more inducers of said inducible origin of replication to the culture medium when the bacterial culture has reached an optical density at 600 nm (OD600) of at least 20, e) further culturing the bacterial cells in the culture medium, f) optionally separating the bacterial cells from the culture medium, and g) recovering the plasmid from the bacterial cells. Also provided herein are vectors with a size of at least 16 kb comprising an inducible origin of replication for use in such methods.

The invention discloses antibacterial coating liquid, a preparation method thereof and an implantable medical device. In preparation of the antibacterial coating liquid, firstly, polyvinyl alcohol and phytic acid are mixed and stirred to realize crosslinking, and then the mixture is mixed with tannic acid and an SBMA zwitterionic solution to prepare the coating liquid. The coating liquid can be loaded on the surface of a substrate through a simple method to prepare an antibacterial coating, and the coating has excellent adhesion performance and can be prevented from falling off in the use process. Due to the excellent hydrophilicity, the adhesion of bacteria, platelets and the like can be effectively reduced. Meanwhile, the phytic acid and cationic groups in the SBMA zwitterions are combined with bacterial cell membranes through electrostatic interaction, so that the bacterial structure is destroyed, and the antibacterial performance of the coating is further enhanced. In an in-vivo implantation experiment, the coating shows good biocompatibility.

This invention discloses a method for preparing and applying a photothermal nanoreagent targeting bacteria. This invention utilizes Ti3C2T... x Using MXene as a matrix, a photothermal nanoreagent MXene@PDA@Peptide (MPP) was constructed by sequentially modifying the surface of Ti3C2Tx MXene with polydopamine and grafting it with the peptide CAEKA, which has the function of recognizing bacteria. Under the recognition of the peptide CAEKA, the photothermal Ti3C2Tx MXene was targeted. x MXene nanosheets are guided to the surface of bacterial cells, achieving effective sterilization at a relatively low overall temperature of around 50°C (or achieving more efficient sterilization at the same overall temperature level, exhibiting superior sterilization performance compared to pure Ti3C2Tx MXene). This makes them particularly suitable for the preparation of therapeutic drugs for subcutaneous abscesses caused by methicillin-resistant Staphylococcus aureus (MRSA) infection.

This invention discloses a composite nanofiber membrane for immobilizing microorganisms to remediate diesel-contaminated water and its preparation method. Specifically, the composite nanofiber membrane is a thermoplastic polyurethane / nanohydroxyapatite composite nanofiber membrane, which not only possesses excellent biocompatibility and mechanical strength but is also non-toxic and harmless, offering considerable cost-effectiveness. Upon introduction into diesel-contaminated water, the composite nanofiber membrane first rapidly adsorbs diesel at the water / oil interface, preventing further diffusion and toxic effects on the aquatic environment. Subsequently, due to the additional adsorption sites provided by the nanoparticles on the membrane, a large number of free bacteria are adsorbed onto the membrane, enhancing the metabolic activity of bacterial cells and their resistance to the toxicity of stubborn compounds, while also strengthening the affinity between hydrophobic compounds and cells, thereby increasing the degradation rate of diesel hydrocarbons by microbial cells. This invention improves the remediation effect of diesel wastewater while expanding the application of composite nanofiber membranes in the field of water pollution treatment.

Antimicrobial eyewear features frames infused with or coated with antimicrobial additives, thereby offering continuous protection by actively destroying and inhibiting the growth of bacteria, fungi, parasites, and some viruses. The lenses may also have an antibacterial coating that destroys and inhibits bacterial growth. In some examples, the lenses are treated with a specialized strengthening liquid, wherein the liquid contains nano components that release heavy metal ions capable of invading the cell walls of bacteria, leading to bacterial elimination, destroying DNA molecules and proteases within the bacterial cells, denature viral proteins, break DNA chains, and result in cell death. Additionally, the strengthening liquid reduces dehydrogenase activity and interacts with various protein groups within the cell, thereby reducing the activity of these groups and effectively inhibiting the growth of E. coli and other bacteria on the lens surface.

The invention discloses a manganese-based layered hydroxide modified by gold nanoparticles and a preparation method thereof, and relates to the technical field of drug synthesis, and the key points of the technical scheme are as follows: the layered morphology of Mn-LDH is observed on the manganese-based layered hydroxide in a transmission electron microscope, and spherical AuNPs dispersed on the surface and edge of a laminate have a uniform particle size and a uniform particle size. EDS energy spectrum analysis finds that gold, manganese, magnesium, aluminum and carbon elements exist, the hydrated particle size is 312 nm, and the dispersity is good. The material can initiate an excellent photo-thermal effect under the irradiation of 808nm near-infrared light to generate active oxygen and hydroxyl radicals to destroy bacterial cell walls and cell membranes, and the antibacterial effect can reach 99%. In addition, the material can stimulate a local active immune function, promote T cell infiltration and realize active immune sterilization. The nano material shows a remarkable anti-infection effect in an in-vitro antibacterial experiment and an in-vivo osteomyelitis animal model experiment, and a new insight is provided for treating osteomyelitis caused by methicillin-resistant staphylococcus aureus.

This invention relates to an antibacterial membrane-penetrating peptide and its applications. The amino acid sequence of the antibacterial membrane-penetrating peptide includes the sequence shown in SEQ ID NO.1 or a sequence with more than 70% homology to the sequence shown in SEQ ID NO.1. This invention screened and obtained a short peptide that specifically binds to the bacterial Hsp70s protein. It was found that this peptide binds to the Hsp70s protein through a traditional substrate-binding pocket, exhibiting an unusually high affinity for the protein in the DnaK-ATP state. The short peptide can inhibit Hsp70s protein activity to a certain extent, preventing it from aiding in the renaturation of denatured luciferase. Furthermore, while maintaining the integrity of the bacterial cell membrane, this short peptide has a superior ability to cross the bacterial cell membrane. The invention further discusses its application potential in the preparation of antibacterial drugs and antibiotic adjuvants, providing new ideas for solving current problems such as bacterial resistance and the difficulty in discovering new antibiotics.

The application belongs to the technical field of non-antibiotic compounds as bacteriostatic agents, and discloses an application of a known non-antibiotic compound, cladrabine, in inhibiting the growth of multi-drug resistant Klebsiella pneumoniae. The application finds that cladrabine can inhibit the growth of multi-drug resistant Klebsiella pneumoniae, and the minimum bacteriostatic concentration reaches 64 mg / L. Further exploration of the bacteriostatic mechanism finds that after exposure to cladrabine, the permeability of the bacterial cell membrane is enhanced, and the macromolecular substances (nucleic acids and proteins) in the cell are excreted, causing the integrity of the bacterial cell membrane to be destroyed, and further causing the death of Klebsiella pneumoniae. The application proposes that cladrabine can destroy the cell membrane of bacteria, change the permeability of the cell membrane, has a good inhibitory effect on multi-drug resistant Klebsiella pneumoniae, and can be further applied to the preparation of related drugs.

This invention discloses a composite antibacterial and oleophobic paper film and its preparation method, belonging to the technical field of food packaging materials. The preparation method provided by this invention uses bacterial cellulose as a base material, adds plasticizers and crosslinking agents, stirs and disperses them, then combines silane-modified starch oleophobic agent, phytic acid, nano-titanium dioxide, and surfactants, mixes them evenly, and obtains the finished product through suction filtration. This method uses bio-based raw materials throughout, discarding difficult-to-degrade harmful components, and the product is naturally biodegradable, solving the waste pollution problem at its source. Phytic acid, through chelating metal ions, destroying bacterial cell membranes, and photocatalytic generation of reactive oxygen species by nano-titanium dioxide, synergistically inhibits bacteria, achieving an antibacterial rate close to 100%. Silane-modified starch with methyl hydrophobic modification combined with the micro-nano rough structure of titanium dioxide significantly improves oleophobic and hydrophobic properties. The composite antibacterial and oleophobic paper film prepared by this method combines biodegradability, high-efficiency antibacterial properties, and excellent oleophobicity, making it suitable for industrial production and food packaging applications.

The disclosure relates to the field of specialty chemicals and methods for their synthesis. In embodiments, the disclosure provides viable bacterial cells which comprise heterologous dual 3-hydroxy-acyl-ACP dehydratase / isomerases, etc. The disclosure further provides monounsaturated fatty acid derivative molecules produced by the viable bacterial cells which are non-native to the bacterial cells. The disclosure further provides methods for the preparation and production of non-native monounsaturated fatty acid derivative molecules such as e.g., an ω3-monounsaturated fatty acid derivative, an ω5-monounsaturated fatty acid derivative, an ω9-monounsaturated fatty acid derivative, an ω11-monounsaturated fatty acid fatty acid derivative, etc.

The invention belongs to the technical field of biology, and discloses virus lysozyme from a deep sea cold spring as well as a preparation method and application of the virus lysozyme. The virus lysozyme provided by the invention comprises amino acid fragments of which the sequences are as shown in one or more of SEQ ID NO: 1-7. The virus lysozyme is derived from a deep sea cold spring virus, the sequence and the structure of the virus lysozyme can keep structural stability and activity in extreme environments such as low temperature and high pressure, and the virus lysozyme has relatively good bacterial cell wall lysis capability and bacteriostatic action; the method has important significance on research and development of bacteriostatic agents under special ecological or technological conditions such as low-temperature food preservation, biological processing and ocean engineering.

The invention discloses a method for killing bovine herpesvirus by sodium chloride aerosol. The method comprises the following steps: (1) preparing sodium chloride aerosol of which the average particle size is 800nm and the particle size distribution is between 500nm and 8mu m, in the specific preparation process, respectively introducing a saturated sodium chloride aqueous solution and absolute ethyl alcohol into dry airflow of a spray dryer according to a volume ratio of 1: 1, and drying by the spray dryer to prepare the sodium chloride aerosol; and (2) the prepared sodium chloride aerosol is used as a bovine herpes virus killing agent to kill bovine herpes viruses, and in the virus killing process, the dosage is 10-60 mg, and the killing time is 10-30 s. The sodium chloride aerosol has the advantages that the sodium chloride aerosol has special antivirus efficacy in a specific dosage and time range, a high-concentration sodium chloride solution can dehydrate bacterial cells and destroy cell membrane structures of the bacterial cells through osmotic pressure difference to cause growth inhibition or death of bacteria, and especially 100% of bovine herpes viruses (BHV-1) can be killed in a very short time with a small dosage of sodium chloride aerosol.

The present disclosure provides an engineered nucleic acid sequence comprising a gene cassette, the gene cassette comprising one or more regulator genes, the regulator genes including an iadR gene, and at least three catabolic pathway genes downstream from the one or more regulator genes, the at least three catabolic pathway genes including an iadC gene, an iadD gene, and an iadE gene, wherein the engineered nucleic acid sequence is free of an iadA gene and iadB gene. The disclosure also provides bacteria cells comprising the engineered nucleic acid sequence, wherein the bacteria cell is capable of degrading indole-3-acetic acid. Methods for degrading auxin, controlling auxin, and promoting plant growth and root development are provided, comprising providing the bacteria cell and allowing the bacteria cell to interact with roots of a plant.

The invention discloses an antibacterial healing-promoting hydrocolloid wound surface auxiliary material and a preparation method thereof, and the antibacterial healing-promoting hydrocolloid wound surface auxiliary material comprises the following components by mass: 30-50 parts of a hydrocolloid matrix; 5 to 12 parts of a modified chitosan-graphene composite antibacterial agent; 3-8 parts of plant source healing promoting active peptide; 4 to 10 parts of a poly gamma-glutamic acid grafted hyaluronic acid moisturizing synergist; 2-6 parts of a plasticizer; 1-4 parts of an adhesion regulator; 0.1 to 0.5 part of a preservative; 15 to 30 parts of deionized water; the antibacterial performance is excellent, the modified chitosan-graphene composite antibacterial agent is adopted, the antibacterial activity of chitosan is enhanced through quaternization modification, then chitosan is compounded with graphene, on one hand, the water solubility and the antibacterial activity of chitosan are enhanced, on the other hand, the high specific surface area and the charge effect of graphene are utilized, and the antibacterial performance of chitosan is improved. The damage capability of the antibacterial agent to bacterial cell membranes is further improved, broad-spectrum and efficient antibacterial effects are achieved, the risk of drug resistance is avoided, and the biological safety is high.

An object is to provide a joint function-improving composition which is useful for prevention or treatment of various arthropathies such as osteoarthritis including knee osteoarthritis as a typical example and rheumatoid arthritis because the composition has an action of promoting growth of chondrocytes and an action of suppressing the production of an inflammation factor, a cartilage matrix degradation factor, a pain factor or a neuronal outgrowth factor by synoviocytes and to provide a joint function-improving product containing the composition such as a food or a drink, feed and a medicine. A joint function-improving composition containing bacterial cells and / or a culture of a bacterium belonging to genera Lactobacillus, Lactococcus, Streptococcus or Bifidobacterium and a joint function-improving agent, a joint function-improving food or drink, a joint function-improving nutritional composition, joint function-improving feed or a joint function-improving pharmaceutical agent which is characterized by containing the joint function-improving composition.

To provide a processed food containing a meat-like ingredient mainly made of plant raw materials and having a meat-like richness imparted thereto, and a method for producing the same. [Solution] A heat-treated, containerized processed food containing a meat-like ingredient, wherein the meat-like ingredient is primarily made of plant-based ingredients including plant protein, and the processed food further contains acetic acid bacteria cells, the plant protein content of the total protein being 90% by mass or more, the mass ratio of the bacterial cells to the plant protein being 0.0005 or more, and the aldehyde dehydrogenase specific activity per 1 mg of the bacterial cells in a dry state being 0.1 U / mg or less.

The present invention relates to a fusion polypeptide comprising an IL10 polypeptide, which can be prokaryotically expressed and secreted in bacterial cells. The present invention also relates to a modified bacterium having anti-tumor activity in tumors and the ability to prokaryotically express and secrete an IL10 polypeptide, wherein the bacterium comprises an essential gene expression cassette controlled by an obligate hypoxia-inducible promoter, and the bacterium is deficient in at least one gene, or its functional expression product, involved in or regulating an endogenous antioxidant stress response pathway. The present invention also relates to a pharmaceutical composition comprising the modified bacterium and its anti-tumor use.

Some aspects of the present disclosure are generally related to systems or methods for identifying cells using hyperspectral imaging or other imaging techniques. According to some embodiments, the system may be configured to perform hyperspectral imaging on a single cell, e.g., a single bacteria cell. In some embodiments, the systems described herein may include a microfluidic device having a plurality of cell growth trenches configured to separate and / or grow cells therein. In some such embodiments, the microfluidic device may facilitate high throughput separations and / or identification of cells from a sample, e.g., by acquiring hyperspectral profiles of cells separated and / or grown in the microfluidic device. Still other aspects of the present disclosure are generally directed to methods of using the systems, kits containing the system, or the like.

Provided herein are methods of determining antibiotic susceptibility of bacteria. In some embodiments, the methods include obtaining a series of images from partitioned samples over a length of time using a large volume scattering imaging (LVSi) technique to produce a set of image data. In some embodiments, the methods include extracting multiple phenotypic features from individual bacterial cells in the partitioned samples in parallel with one another and detecting one or more phenotypic feature changes associated with bacterial growth and response to the antibiotics in the set of image data over a selected period of time to produce a set of extracted phenotypic features, and passing the set of extracted phenotypic features through a trained machine learning model that predicts the identity and the antibiotic susceptibility of the bacterial cells in the partitioned samples. Related kits, devices, systems, computer readable media, and additional methods are also provided.

This invention provides a detection method for distinguishing between live and dead bacteria of the watermelon fruit spot bacterial disease fungus. [Solution] A detection method for distinguishing between live and dead bacteria based on PMA-PCR technology, comprising: a step of collecting and suspending bacteria in a sample to be detected, which involves suspending the sample in physiological saline to obtain a bacterial suspension and heating it in a 100°C water bath for 10 minutes to prepare a dead bacterial suspension; a step of PMA treatment, which involves adding propidium monoazide (PMA) to the bacterial suspension; a step of blue light crosslinking, which involves irradiating the PMA-treated bacterial suspension with blue light to activate the PMA and covalently bind it to the dead bacterial DNA; a step of DNA extraction, which involves collecting bacterial cells by centrifugation and extracting DNA using a kit method; a PCR amplification step, which involves amplification using a specific primer pair; and a step of detecting the amplified product by agarose gel electrophoresis, which involves determining the result, which involves a step of detecting the product by agarose gel electrophoresis, wherein a target band appears in the live bacterial sample and no band appears in the dead bacterial sample.

The invention relates to the technical field of banana leaf spot disease control, and discloses an epoxiconazole and saisentong mixed composition and application to banana leaf spot disease treatment, the epoxiconazole and saisentong mixed composition comprises the following components by mass: 26%-34% of an active composition, 1%-3% of a wetting agent, 3%-7% of a dispersing composition, 0.1%-0.5% of a thickening agent, 0.3%-0.8% of magnesium aluminum silicate, and the balance of deionized water, and the active composition is composed of epoxiconazole and saisentong. Fungus ergosterol synthesis is inhibited through a triazole action mechanism of epoxiconazole, meanwhile, copper ions released by Saisentong are combined to destroy a bacterial cell membrane protein structure, double killing of banana cephalosporium and pathogenic variant bacteria is achieved, broad-spectrum prevention and control of fungi and bacteria are achieved through synergistic compounding of epoxiconazole and Saisentong, and the synergistic effect of epoxiconazole and Saisentong is achieved. Therefore, the limitation of a single agent is broken through, efficient broad-spectrum prevention and control on a mixed pathogen system are realized, and the development of drug resistance is obviously delayed.

The application discloses an additive for enhancing immunity of livestock and poultry, a preparation method and application thereof. The additive for enhancing immunity of livestock and poultry is combined with a polymer and an antibacterial peptide, and the stability and amphiphilicity of the antibacterial peptide are increased. In the additive for enhancing immunity of livestock and poultry, a flexible polymer chain is connected to a peptide main chain to form an amphiphilic conformation, and the additive has more excellent activity than a rigid secondary structure formed by only the peptide chain. The additive for enhancing immunity of livestock and poultry has strong bioactivity and high permeability to a bacterial cell membrane, and has excellent bactericidal effect by destroying the cell membrane of the bacteria, so that the additive can be used in livestock and poultry feed, can enhance the immunity of the livestock and poultry, can effectively prevent and treat diseases, and can promote the growth of the livestock and poultry.

This invention provides novel compositions and methods for effectively stimulating antiviral immunity. [Solution] A composition comprising (a) a vector comprising a plasmid encoding at least one viral antigen; and (b) a vector comprising a CD1d recognition antigen; and (c) at least one pharmaceutically acceptable carrier, wherein at least one of vectors (a) and (b) is an intact bacterial minicell or a dead bacterial cell.

An object of the present invention is to provide a packaged acidic beverage according to the present invention such that a “sour taste” specific to an acidic beverage containing GABA in a prescribed concentration is suppressed, a production method therefor, and others. Provided is a packaged acidic beverage comprising γ-aminobutyric acid (GABA) at 100 mg / L or more and 500 million cells / L or more (e.g., 20 billion cells / L or more) of dead bacterial cells of one or more types of lactic acid bacteria and / or bacteria in the present invention 1 and having a pH of 4.6 or less.

A method for producing γ-Glu-Val-Gly is described, wherein the method includes the steps of cultivating a γ-Glu-Val-Gly-producing bacterium belonging to the family Enterobacteriaceae in a culture medium so that the γ-Glu-Val-Gly accumulates in the culture medium or the cells of the bacterium, or both, and collecting the γ-Glu-Val-Gly from the culture medium or the cells of the bacterium, or both. The bacterium has been modified to overexpress a gene encoding a protein having L-threonine 3-dehydrogenase activity and a gene encoding a protein having 2-amino-3-oxobutanoate coenzyme A ligase activity.

The invention relates to cells, such as bacterial cells, comprising orthogonal DNA replication machinery, linear plasmids that may be replicated by said machinery, uses of said cells and said linear plasmids, methods of maintaining linear plasmids in cells, methods of evolving sequences of interest, and methods of making polypeptides or nucleic acids. Orthogonal systems that are based on or derived from phage, such as phi29, that infect gram-positive bacteria are disclosed herein.

The present disclosure relates to a conjugated prodrug comprising a peptide conjugated to an antibiotic molecules via a cleavable linker and pharmaceutical compositions thereof. Also disclosed are methods of enhancing the intracellular concentration of an antibiotic agent in a bacterium and methods of treating a patient for a bacterial infection.