331 results about "Bacteriocyte" patented technology

Disclosed are methods of producing eukaryotic disulfide bond-containing polypeptides in bacterial hosts, and compositions resulting therefrom. Co-expression of a eukaryotic foldase and a disulfide bond-containing polypeptide in a bacterial host cell is demonstrated. In particular embodiments, the methods have been used to produce mammalian pancreatic trypsin inhibitor and tissue plasminogen activator (tPA) in soluble, biologically-active forms, which are isolatable from the bacterial periplasm. Also disclosed are expression systems, recombinant vectors, and transformed host cells.

Disclosed are methods and compositions for producing heterologous disulfide bond containing polypeptides in bacterial cells. In preferred embodiments the methods involve co-expression of a prokaryotic disulfide isomerase, such as DsbC or DsbG and a gene encoding a recombinant eukaryotic polypeptide. Exemplary polypeptides disclosed include tissue plasminogen activator.

The inventive method allows peptides or polypeptides to be exposed on the surface of gram-negative host bacteria using specific intimin-based anchor modules. Intimins with shortened carboxy terminals have been found to be particularly suitable anchor modules for passenger domains in the exterior E. coli cell membrane. According to the method, host bacteria are transformed using vectors, on which are located a fused nucleic acid sequence consisting of a sequence segment which codes for an intimin with a shortened carboxy terminal and a nucleic acid sequence segment which codes for the passenger peptide that is to be exposed. The invention permits a particularly large number of passenger domains to be exposed on the cell surface of the bacteria, without adversely affecting the viability of the bacteria.

A film-shaped polymer matrix includes lactic acid producing bacteria that are dissolved when exposed to wet conditions. The film-shaped polymer matrix protects bacterial cells from moisture thereby increasing bacterial survival during transport and storage. The film-shaped polymer matrix also results in a high transfer of bacterial cells to the skin of a subject.

Existing methods of meningococcal OMV preparation involve the use of detergent during disruption of the bacterial membrane. According to the invention, membrane disruption is performed substantially in the absence of detergent. The resulting OMVs which retain important bacterial immunogenic components, particularly (i) the protective NspA surface protein, (ii) protein NMB2132 and (iii) protein NMB 1870. A Typical process involves the following steps: (a) treating bacterial cells in the substantial absence of detergent; (b) centrifuging the composition from step (a) to separate the outer membrane vesicles from treated cells and cell debris, and collecting the supernatant; (c) performing a high speed centrifugation of the supernatant from step (b) and collecting the outer membrane vesicles in a pellet; (d) re-dispersing the pellet from step (c) in a buffer; (e) performing a second high speed centrifugation in accordance with step (c), collecting the outer membrane vesicles in a pellet; (f) re-dispersing the pellet from step (e) in an aqueous medium.

The invention relates to compositions for treating parasitic worm infections in a mammal. The composition includes an amount of intracellular components of lysed, beneficial, soil-inhabiting yeast cells sufficient to treat parasitic worm infections in a mammal; and/or an amount of whole or lysed soil-inhabiting bacteria cells, wherein the amount is sufficient to treat parasitic worm infections in a mammal, and optionally a pharmaceutically suitable carrier.

The present disclosure provides a ready-to-use bio-electrode stable for long term storage and a process of constructing the same. The process for construction of bio-electrode for electro-biocatalysis comprising of: selection of an electro-active bacteria; enrichment of said electro-active bacteria in a nutrient rich medium; separation of said electro-active bacterial cells from said nutrient rich medium; selection of an electrode material; surface modification of said electrode material; layering the surface modified electrode material with conductive material; layering the surface modified electrode material with an electro-active bacterial cells along with biofilm inducing agents and stabilizing agents; conditioning the electro-active bacterial cells layered electrode; incubating the electrode obtained with an immobilizing agent along with conductive material; and conditioning the electrode with micronutrients to obtain a bio-electrode.

The invention relates to a process for the production of L-amino acids, in which the following steps are carried out: a) fermentation of the coryneform bacteria producing the desired L-amino acid, in which at least the mqo gene is attenuated, b) concentration of the desired L-amino acid in the medium or in the cells of the bacteria, and c) isolation of the L-amino acid, and, optionally, bacteria are used in which further genes of the biosynthesis pathway of the desired L-amino acid are additionally enhanced, or bacteria are used in which at least some of the metabolic pathways reducing formation of the desired L-amino acid are excluded.

Methods for increasing the resistance to rumen inactivation of a cultured Gram positive bacteria strain useful for gastrointestinal delivery of bioactive compounds to ruminants, which includes the steps of growing a culture of the bacteria strain through at least one passage in a growth medium containing an amount of lysozyme effective to induce the growth of bacterial cell walls resistant to protozoal predation; and recovering the bacteria strain from the lysozyme-containing medium. Rumen-bypass feed supplements produced by the inventive method are also disclosed, as well as methods for supplementing the diet of a ruminant with the rumen bypass feed supplements and an in vitro method for evaluating the resistance of Gram positive bacteria strains to rumen inactivation in vivo.

The invention belongs to the field of biological medicine, and particularly relates to composite hydrogel for promoting wound healing as well as a preparation method and application thereof. The composite hydrogel is composed of gelatin, antibacterial photodynamic peptide and recombinant human III-type collagen. A photosensitizer Ce6 is introduced for photodynamic antibacterial chemotherapy antibiosis, and the photosensitizer Ce6 generates active oxygen under laser irradiation to cause microbial molecule oxidation and bacterial cell damage and death, so that the photosensitizer Ce6 and antibacterial peptide generate a synergistic effect. The antibacterial composite hydrogel has high biocompatibility, keeps a wet environment around a wound surface, can stably convey loaded antibacterial photodynamic peptide and recombinant human collagen to the surface of the wound, guides fibroblasts to migrate and proliferate and increases deposition of new collagen on blood vessels for reconstructionin a dermal repair process, thereby accelerating wound healing, thereby improving wound healing quality.

The invention is directed compositions and methods related to bacterial cells that are physically associated with ceramide-like glycolipids for use as antigen carriers for heterologous antigens. The invention further relates to methods of incorporating ceramide-like glycolipid to bacterial cell walls. The compositions and methods of the present invention are useful for the prevention and treatment of diseases.

This invention relates to a bacterial membrane agent and methods therefore. The membrane bacterial strain is labeled by color fluorescent protein gene. The said agent and methods verify in-situ analysis, i.e., analyzing under a condition of non-destructing biologic membrane; easy operation, i.e., no need of microbe culture and counting or extraction of cell nucleic acid for cross-breed or electrophoretic determination; and good real time online analysis.

The existing extraction and purification method of polyhydroxyalkanoate (PHA) comprises: adding hydrolase and the like into an organic solvent or a surfactant, and is complex and expensive in process.According to the invention, according to the method, a PHA-rich bacterial fermentation broth is treated with hot water or superheated water with a temperature of more than 100 DEG C so as to completewall breaking and purification of bacterial cells, and surfactant, protease and/or bleaching agent treatment and the like can also be combined to further improve the purity of PHA; and the method hasthe advantages of low cost, simple operation and high product recovery rate, and overcomes the problems of complex steps, high cost of addition of chemical substances, difficult treatment of downstream sewage and the like of the traditional PHA extraction.

The invention discloses polypeptide and a preparation method and application thereof with an anti-bacterial function and belongs to the technical field of anti-bacteria. The antibacterial polypeptide comprises amino acid sequences as shown in the SEQ ID NO.1 and salt or ester thereof. The antibacterial polypeptide forms an alpha spiral structure through the specific array of specific kinds of amino acid, the spiral structure makes contact with and acts on a bacterial cell wall to cause the piercing of the cell wall, the bacterium dies, and therefore the antibacterial effect is achieved. The antibacterial polypeptide is convenient to composite, low in cost, good in effect and safe and reliable. Meanwhile, protein antibacterial agents can effectively reduce the occurrence of bacterial drug resistance, and huge potential is achieved in the direction of replacing antibiotics to be the main stream antibacterial medicine.

The invention relates to an aquaculture animal disease control technology and in particular relates to a tilapia mossambica source streptococcus agalactiae low virulent strain and application thereof. The tilapia mossambica source streptococcus agalactiae low virulent strain is YM001 and is preserved in CCTCC (China Center For Type Culture Collection) with the preservation number of CCTCCM2014045. The tilapia mossambica source streptococcus agalactiae low virulent strain is subjected to activation culture and fermentation culture, bacterial cells of a 106-109CFU/mL low virulent strain YM001 and a sterile phosphate buffered solution are mixed as a low virulent live vaccine used for preventing and controlling a tilapia mossambica streptococcus disease, the injection medication concentration for each tilapia mossambica is 105-108CFU, the oral medication concentration for each tilapia mossambica is 108-109CFU, and the soaking medication concentration is 107-108CFU/mL. The invention relates to strain identification, toxicity test, toxicity return safety and stability, immunogenicity and immune protection effect of a low virulent strain, and the tilapia mossambica streptococcus disease vaccine prepared by adopting the low virulent strain is good in immune protection effect and strong in safety and stability.

The invention belongs to the technical field of preparation of nano materials, and particularly relates to preparation of a mimic oxidase and application of the mimic oxidase in photocatalysis, bacteriostasis and sterilization. According to the mimic oxidase, iopromide and ethylenediamine are used as raw materials; nitrogen-doped and iodine-doped carbon dots are synthesized by a one-step hydrothermal method; the enzyme characteristics are simulated based on synthetic carbon dots; the nitrogen-doped and iodine-doped carbon dots are mixed with hydrogen peroxide to form a novel antibacterial agent; coexistence of carbon dot nano-enzyme and hydrogen peroxide is utilized; under visible light irradiation, the oxidation activity of the nano-enzyme can promote the oxidation process of bacterial cells and the consumption of antioxidant biomolecules; the antibacterial activity of oxygen products including H2O2 and other accumulated antibacterial activities from reactive oxygen species (ROS) is reduced, a good antibacterial effect is generated, and due to the characteristics of safety, good biocompatibility and the like of the developed novel antibacterial agent, a great application prospectis achieved.

Iodo-cosanol and ethylenediamine are used as raw materials to synthesize nitrogen-doped and iodine-doped carbon dots by a one-step hydrothermal method, the enzyme characteristics are simulated based on the synthesizedcarbon dots, nitrogen and iodine doped carbon dots are fixed on the surfaces of chitosan molecules with high biological matching performance, and the formed compound is mixed with hydrogen peroxide to be used as a novel antibacterial agent; the carbon dot nano-enzyme and hydrogen peroxide coexist, and the oxidation activity of the nano-enzyme promotes the oxidation process of bacterial cells and the consumption of antioxidant biomolecules so that the oxygen products including H2O2 and other accumulated antibacterial activities from reactive oxygen species (ROS) are reduced; meanwhile, the chitosan also has better antibacterial property and generates a synergistic antibacterial effect. The antibacterial agent is mixed with printing ink to be used for resisting bacteria of tipping paper for cigarettes, the antibacterial performance of the antibacterial agent meets the requirements of the tobacco industry, and the antibacterial agent is a safe, environment-friendly and effective novel antibacterial agent.

A method of inhibiting bacterial cell growth or of treating or inhibiting a bacterial infection in a subject by administering to the subject a therapeutically effective amount of a polynucleotide encoding a polypeptide having α/β small acid-soluble spore protein (SASP) activity in a delivery system which targets a bacterial cell.

The invention discloses a baby wet wipe and a preparation method thereof. The baby wet wipe is prepared by immersing a non-woven fabric into a mixed solution of a first emulsion and a second emulsion,and an antibacterial agent is prepared in the preparation process; the antibacterial agent can react with head groups of acidic phospholipids in bacterial cell membranes, so that the permeability ofthe cell membranes is reduced, bacterial cell liquid leaks, and bacterial cells are dead; partial carboxyl on modified graphene is condensed with amino on dopamine, then gamma-aminopropyltriethoxysilane is added to be condensed with residual carboxyl on the modified graphene, and finally hydrolysis is performed, so that siloxane on the gamma-aminopropyltriethoxysilane is hydrolyzed and connected,and a modified non-woven fabric is prepared; and the modified non-woven fabric has relatively good moisture retention property, high toughness and low possibility of damage.