277 results about "Bacteriocyte" patented technology

The invention discloses antibacterial poly (beta-amino ester) and a preparation method and application thereof, poly (beta-amino ester) macromolecules simultaneously have amino positive charges and fluorine-containing hydrophobic components, existence of the positive charges enables the poly (beta-amino ester) macromolecules to be more easily combined with negatively charged bacterial cell membranes and to be enriched, and the hydrophobic components can enable the poly (beta-amino ester) macromolecules to be inserted into cell membrane phospholipid bilayers, so that the antibacterial effect is improved. Therefore, bacteria are effectively killed, and drug resistance is not easily caused. The polymer has high antibacterial property and low hemolytic toxicity, can be self-assembled into nano particles, and can be combined with antibiotics in an antibiotic loading manner to improve the treatment effect. The compound can be synthesized through simple steps, the reaction condition is simple, the preparation cost, the raw material cost and the application cost are very low, and the compound has a very good prospect when being applied to medicines for treating or preventing diseases caused by bacteria.

The invention discloses a preparation method of photo-thermal-MMP dual-response matrix hydrogel and application of the photo-thermal-MMP dual-response matrix hydrogel in diabetic wound repair, and belongs to the technical field of biological medicine. According to the present invention, the Cur (at) ZIF-8 (at) MMPDA / dAMG composite hydrogel is composed of an amnion source photo-crosslinked matrix hydrogel, curcumin-loaded ZIF-8 nanoparticles and MMP2 peptide modified polydopamine, and the Cur (at) ZIF-8 (at) MMPDA / dAMG composite hydrogel is obtained; the ZIF-8 nanoparticles decline in an acid environment to release zinc ions, destroy bacterial cell membranes, enhance the permeability of a biological membrane and cooperatively kill drug-resistant bacteria in combination with a photothermal effect, Cur is loaded through ZIF-8 to form Cur-coated ZIF-8, then a Cur-coated ZIF-8-coated MMPDA / dAMG double-drug-loading system is constructed through an MMPDA medium, and a photothermal antibacterial, anti-inflammatory and regeneration-promoting multi-mode therapy is formed for a wound surface in the acid environment. Therefore, wound healing is accelerated.

The invention provides engineering lyase Art-15 with broad-spectrum antibacterial activity, antibacterial peptide NZ2114 and bacteriophage lyase PlySs2 are subjected to functional fusion through an optimally designed flexible linker, and an efficient bactericidal effect on Staphylococcus aureus (Staphylococcus aureus) and Streptococcus spp.) is achieved. The engineering lyase Art-15 has the advantages that the engineering lyase Art-15 has broad-spectrum antibacterial activity, the antibacterial peptide NZ2114 and the bacteriophage lyase PlySs2 are subjected to functional fusion through the optimally designed flexible linker, and the engineering lyase Art-15 has broad-spectrum antibacterial activity; the lyase Art-15 overcomes the defects of narrow host spectrum and insufficient splitting activity of the lyase through a synergistic destruction mechanism of targeting bacterial cell wall peptidoglycan. Experiments show that the minimal inhibitory concentration (MIC) of the lyase Art-15 on staphylococcus aureus in vitro is lower than 2 mu g / mL, the minimal inhibitory concentration of the lyase Art-15 on streptococcus agalactiae is lower than 1 mu g / mL, the minimal inhibitory concentration of the lyase Art-15 on streptococcus dysgalactiae is lower than 2 mu g / mL, and the sterilization rate of the lyase Art-15 can reach 97% or above within 30 minutes by 2 times of MIC. The lyase Art-15 disclosed by the invention has important application value in the fields of drug-resistant bacterium infection treatment, medical instrument disinfection and livestock breeding.

The invention discloses a composite antibacterial preparation containing antibacterial peptide and traditional Chinese medicines as well as a preparation method and application of the composite antibacterial preparation, and belongs to the technical field of biological medicines. The antibacterial peptide provided by the invention is a transmembrane protein derived from bacteriophage, and the core function of the antibacterial peptide is that bacterial cell lysis is promoted by destroying a connection structure of a peptidoglycan layer and lipoprotein of a bacterial cell wall, so that an antibacterial effect is achieved. The amino acid sequence of the antibacterial peptide is shown as SEQ ID NO: 3, the antibacterial peptide is combined with traditional Chinese medicines to form a composite antibacterial preparation, and experiments prove that the composite antibacterial preparation has a relatively good antibacterial effect on gram-positive bacteria and gram-negative bacteria and can be applied to the medical field, such as surgical instrument disinfection and drug-resistant bacterial infection treatment; the field of daily chemicals, such as antibacterial hand sanitizers and wound dressings; in the field of agriculture, for example, animal feed additives for preventing and treating intestinal infection have a good market application prospect.

The present disclosure provides engineered bacterial cells having one or more genetic modifications that result in increased production of expression products, such as increased production of plasmids with reduced occurrence of plasmid concatemers or multimers. Also disclosed herein are expression systems for product manufacturing in media having reduced antibiotic concentration, as well as methods of making cells for use in the expression systems. The cells and expression systems increase the growth rate and production yields of biomolecules produced within the host bacterial host cells without the use of antibiotics, and can produce the product with little to no endotoxins present.

The present disclosure provides engineered bacterial cells having one or more genetic modifications that result in increased production of expression products, such as increased production of plasmids with reduced occurrence of plasmid concatemers or multimers. Also disclosed herein are expression systems for product manufacturing in media having reduced antibiotic concentration, as well as methods of making cells for use in the expression systems. The cells and expression systems increase the growth rate and production yields of biomolecules produced within the host bacterial host cells without the use of antibiotics, and can produce the product with little to no endotoxins present.

The invention discloses a biodegradable light-package high-barrier packaging film and a preparation process, and relates to the technical field of packaging films, the biodegradable light-package high-barrier packaging film comprises the following components by weight: 8-15 parts of modified montmorillonite, 30-50 parts of modified nano cellulose, 5-10 parts of polyvinyl alcohol, 1.5-3 parts of a plasticizer, 0.25-0.6 part of curcumin, and 40-60 parts of deionized water. According to the biodegradable light-package high-barrier packaging film and the preparation process, montmorillonite is modified, so that the montmorillonite can interact with anions on a bacterial cell membrane, the integrity of the cell membrane is destroyed, and bacterial contents are leaked, thereby inhibiting growth and reproduction of bacteria and improving the antibacterial property of the packaging film; montmorillonite is a natural mineral material, is environment-friendly, and can change the crystal structure of polyvinyl alcohol, so that the degrading enzyme is easier to contact with a polymer molecular chain, and the degradation speed is accelerated.

The invention relates to the technical field of protective films, and discloses a mobile phone protective film and a preparation method thereof. Modified graphene oxide is used as a filler, a modified waterborne polyurethane emulsion is used as a film forming substance, a flatting agent and a defoaming agent are used as auxiliary materials, a waterborne polyurethane coating is prepared, the surface of a PET base material is coated with the waterborne polyurethane coating, then drying is conducted, and the mobile phone protection film is obtained. A quaternary ammonium salt group and negative charges on a bacterial cell membrane generate electrostatic attraction, so that the original structure and function of the cell membrane are destroyed, and a bactericidal effect is achieved; silanol groups generated by hydrolysis of silane groups can react with hydroxyl groups and carboxyl groups on the surface of a PET base material, so that the interface bonding force between the coating and the base material is improved; nitrogen atoms in pyrrolidone groups form hydrogen bonds with hydroxyl groups and carboxyl groups on the surface of a base material, so that the bonding strength of the coating and the base material is further improved; the adamantyl group is grafted into the graphene oxide, so that the supporting effect of the graphene oxide on the waterborne polyurethane is enhanced, and the mechanical property of the mobile phone protective film is improved.

The invention relates to the technical field of antibacterial agents, in particular to a compound antibacterial agent of a plant polyphenol complex and a preparation method of the compound antibacterial agent. The compound antibacterial agent is prepared from the following raw materials in parts by weight: 20-30 parts of moutan bark extract, 20-30 parts of folium mori, 20-30 parts of a carrier, 10-20 parts of jerusalem artichoke extract, 10-20 parts of coptis chinensis extract, 2-4 parts of a tackifier and 1-3 parts of a microbial agent. According to the invention, paeonol in moutan bark, chlorogenic acid in mulberry leaves, berberine in coptis chinensis and inulin polyphenol in jerusalem artichoke can be combined with lipid of bacterial cell membranes, so that membrane permeability is increased, microbial metabolic enzymes are interfered, energy metabolism and biosynthesis are blocked, free radicals are removed, and antibacterial effects of other components are enhanced; folium mori is hydrolyzed through beta-glucosidase to release free polyphenol, gluconolactone adjusts the acid environment to promote polyphenol dissolution, and the antibacterial effect of the composite antibacterial agent is effectively improved.

The invention provides an antibacterial peptide CN-3 and application thereof, and relates to the technical field of peptide inhibitors. The amino acid sequence of the antibacterial peptide CN-3 is GPGGAWAAKVIS, and is as shown in SEQ ID NO. 1. The minimum inhibitory concentration (MIC) of the antibacterial peptide CN-3 to staphylococcus aureus and escherichia coli is 5.0 mg / mL. According to the antibacterial peptide CN-3 and the application thereof, the CN-3 has staphylococcus aureus and escherichia coli bacteriostatic activity, and plays a role by destroying bacterial cell membranes; cN-3 is not easy to generate drug resistance, low in cytotoxicity and high in cell selectivity, and can be used for preparing an antibacterial composition for treating staphylococcus aureus and escherichia coli infection.

The invention discloses bacteriostatic and antibacterial lithium disilicate glass ceramic and a preparation method thereof. The bacteriostatic and antibacterial lithium disilicate glass ceramic comprises the following components in percentage by weight: 60-70% of lithium disilicate whiskers, 5-12% of a nucleating agent, 1-5% of aluminum oxide, 1-4% of calcium oxide, 5-10% of an antibacterial agent and 12-18% of fluorapatite, wherein the nucleating agent is formed by compounding phosphorus pentoxide and zirconium oxide, and the antibacterial agent is formed by compounding silver oxide and zinc oxide; the invention relates to the technical field of lithium disilicate glass ceramics. In the glass ceramic, silver ions can be combined with groups such as sulfydryl and amino in bacterial cells to interfere the metabolic process of bacteria and inactivate enzymes of the bacteria so as to inhibit growth and reproduction of the bacteria, and zinc ions can influence permeability of bacterial cell membranes and destroy physiological functions of the bacteria and cooperate with the silver ions to enhance the antibacterial effect of the glass ceramic.

The invention discloses an intelligent quintuplet cooperative plasma self-purification robot long-term storage system and a self-adaptive disinfection control method. According to the system, cold plasma, ozone, chlorine dioxide, self-generated nitric oxide and a charge self-purification jump effect are fused, and a dynamic self-purification system with an electric field-gas field-chemical field-intelligent field-thermal field five-dimensional synergistic energy field is constructed. The robot adopts a three-section structural layout and a multi-mode self-adaptive propulsion mechanism, environmental parameters are sensed through AI, discharge power and oxidant release are intelligently regulated and controlled, and precise sterilization, toxicity reduction and mildew prevention in a multi-medium environment are achieved. The system can generate various high-energy active species containing. OH, O, NO, ClO, ONOO and the like, chain splitting decomposition and oxidation mineralization are carried out on mold spores, bacterial cells, aflatoxin and the like, the sterilization rate reaches 99.995%, and the toxin degradation rate exceeds 98%. The system has the characteristics of no residue, low energy consumption, long-acting self-maintenance and the like, is suitable for multiple scenes of grain storage, traditional Chinese medicinal materials, cold chains, farmland, public health and the like, and realizes an intelligent, continuous and chemical-additive-free green storage and dynamic purification system.

The invention relates to the field of biological environmental protection, in particular to a heterotrophic nitrification immobilized engineering bacterium for efficient denitrification and a preparation method and application thereof.According to the heterotrophic nitrification immobilized engineering bacterium for efficient denitrification and the preparation method and application of the heterotrophic nitrification immobilized engineering bacterium, polyethylene glycol-glucan two-aqueous-phase concentration and trehalose protection liquid are adopted for synergistic effect, the bottleneck of cell damage caused by a traditional flocculation method can be avoided, trehalose molecules can wrap bacterial cells, and the bacterial cells can be effectively degraded; the immobilized particles can resist osmotic pressure impact and pollutant invasion, the survival rate of thalli is higher than 95%, so that the immobilized particles always keep ultrahigh denitrifying enzyme activity in various sewage environments, honeycomb-shaped pores are formed in an ethyl cellulose matrix, a microenvironment with a high specific surface area is provided for thalli attachment, and the built-in nano zeolite continuously and slowly releases Cu < 2 + > and Zn < 2 + > trace elements, so that the immobilized particles can be recycled. A thallus metabolism enzyme system is synergistically activated, and the denitrification pathway function is enhanced; the chitosan-polydopamine outer coating layer strongly captures the nitrogen-containing pollutants, so that the removal efficiency of the nitrogen-containing pollutants is improved; through triple protection, the density of unit granular bacteria is up to 109 cells / cm, the service life is prolonged, and the denitrification performance is improved by more than 40%.

The invention relates to the technical field of non-woven fabric preparation, in particular to biochemical modified antibacterial non-woven fabric and a preparation method thereof.After nano-zinc oxide and nano-titanium dioxide are modified through a silane coupling agent KH-570, the dispersity of nano-particles can be improved, and the antibacterial property of the non-woven fabric is improved; under illumination or contact conditions, active oxygen is released to destroy bacterial cell membranes and inhibit metabolism of the bacterial cell membranes, so that the antibacterial modified polypropylene master batch has stable antibacterial property; loading is stabilized through a polydopamine coating, so that the antibacterial performance of the lysozyme is stabilized, the lysozyme can specifically hydrolyze a peptidoglycan layer of a bacterial cell wall to cause bacterial lysis, thiourea dioxide serving as a reducing agent can maintain the activity of the lysozyme, oxidative inactivation is avoided, meanwhile, the activity of bacterial metabolic enzymes is inhibited, and the antibacterial activity of the lysozyme is improved. Through the inorganic / biological double-effect synergistic effect, gram-positive bacteria, gram-negative bacteria and fungi can be covered, so that the antibacterial performance of the non-woven fabric is improved to a certain extent.

The invention discloses a photoresponse-essential oil synergistic antibacterial polymer and an application thereof in resisting multiple drug-resistant bacteria. Citronellol (CT) is subjected to an esterification reaction to prepare a CMA monomer, the CMA monomer and a glycidyl methacrylate (GMA) monomer are copolymerized to prepare a polymer PG9C2, and then TBO is introduced to obtain the antibacterial polymer PG9C2T1. The improvement of the antibacterial activity of the TBO is derived from the improvement of the fat solubility of the TBO, the enhancement of the interaction between the TBO and bacterial cells, the improvement of the accumulation of the TBO in the cells, the penetration of the TBO through cell membranes and the play of roles in the cells, the improvement of the utilization efficiency of ROS, and the sterilization by improving the water solubility of the essential oil through the mechanism of destroying bacterial membranes. According to the invention, high-efficiency and low-toxicity drug-resistant bacteria based on natural products are synthesized, the limitation of a single antibacterial mode is broken through, and technical support is provided for developing a novel low-drug-resistance antibacterial therapy.

The invention provides a weakly alkaline strontium-rich mineralized filter element with antibacterial property and a preparation method thereof, and relates to the technical field of water purification filter elements, the weakly alkaline strontium-rich mineralized filter element with antibacterial property comprises the following components by weight: 5-15 parts of modified celestite, 2-10 parts of modified strontianite, 2-5 parts of modified dolomite, 10-25 parts of modified medical stone, and 10-25 parts of modified zeolite, 5 to 15 parts of modified tourmaline, 2 to 10 parts of modified serpentine and 2 to 10 parts of modified wollastonite. After tourmaline is modified, weak current is generated by utilizing thermoelectricity and piezoelectricity to destroy bacterial cell membrane potential, so that the bacterial cell membrane potential is disordered, released negative ions change the physicochemical property of a bacterial living environment, the membrane permeability of bacterial cells is increased, and medical stone and zeolite are modified to release trace elements more easily. Bacterial cells which interfere with membrane permeability increase can be more quickly removed, the metabolism of bacteria is influenced, and the antibacterial effect is achieved.

The invention belongs to the technical field of antibacterial medicines, and particularly relates to a heliotropin-modified ruthenium polypyridine complex as well as a preparation method and application thereof. The structural formula of the ruthenium polypyridine complex is shown as a formula I. According to the polypyridine ruthenium complex modified by the heliotropin structure, the bacterial cell membrane penetrating capacity and the retention effect of the polypyridine ruthenium complex are enhanced. As the ruthenium complex has a rigid octahedral geometric structure, the structure is easier to modify, so that the ruthenium complex can more easily optimize the binding affinity of cell targets compared with organic drugs; the polypyridine structure is a typical nitrogen-containing heterocyclic compound, so that the polypyridine structure has more target spots, and can be used for breaking bacterial cell membranes, thereby inhibiting the generation of bacterial toxins; and the formula I of the # imgabs0 # is shown in the specification.

In various embodiments, provided herein are host cells, methods, and pharmaceutical compositions comprising gastrodin, wherein the gastrodin is produced by a genetically modified plant or plant cell, fungal cell, yeast cell, insect cell, or bacterial cell. In certain embodiments, the present disclosure provides methods and compositions for the production of gastrodin. In yet other embodiments, the present disclosure provides enhanced cells and methods for producing gastrodin.

The present disclosure provides methods and tools that allow for the production of large quantities of double-stranded RNA (dsRNA) in bacterial cells, including plasmid vectors that can be used to transform Gram-positive or Gram-negative bacterial cells, such that the cells produce high yields of target dsRNA.

The present invention relates to the technical field of home textile fabric preparation, and in particular to an antibacterial and anti-mite home textile fabric and a preparation process thereof. The preparation process comprises the following steps: preparing a zinc zeolite carboxylic acid composite material; preparing purified nano-platinum; and preparing an antibacterial and anti-mite home textile fabric. The present invention forms a zinc zeolite structure similar to zeolite by compounding zinc with methylimidazole, and adds 1,3,5-benzenetricarboxylic acid to the zinc zeolite structure to form a composite metal organic framework, which can enhance the contact area with bacteria, so that 1,3,5-benzenetricarboxylic acid has the opportunity to contact key metabolic enzymes in bacterial cells and inhibit their activity. At the same time, the composite metal organic framework can slowly release zinc ions over time after combining with zinc ions, and the slowly released zinc ions have antibacterial properties, can interfere with the metabolic process of bacteria, and inhibit their growth, thereby achieving a long-lasting antibacterial effect.

Provided herein are methods for the production of a vector with a size of at least 16 kb from bacterial cells comprising the consecutive steps of a) obtaining bacterial cells comprising a vector with a size of at least 16 kb, comprising an inducible origin of replication, b) inoculating culture medium with the bacterial cells comprising the vector, c) culturing the bacterial cells in the culture medium, d) adding one or more inducers of said inducible origin of replication to the culture medium when the bacterial culture has reached an optical density at 600 nm (OD600) of at least 20, e) further culturing the bacterial cells in the culture medium, f) optionally separating the bacterial cells from the culture medium, and g) recovering the plasmid from the bacterial cells. Also provided herein are vectors with a size of at least 16 kb comprising an inducible origin of replication for use in such methods.

The invention discloses antibacterial coating liquid, a preparation method thereof and an implantable medical device. In preparation of the antibacterial coating liquid, firstly, polyvinyl alcohol and phytic acid are mixed and stirred to realize crosslinking, and then the mixture is mixed with tannic acid and an SBMA zwitterionic solution to prepare the coating liquid. The coating liquid can be loaded on the surface of a substrate through a simple method to prepare an antibacterial coating, and the coating has excellent adhesion performance and can be prevented from falling off in the use process. Due to the excellent hydrophilicity, the adhesion of bacteria, platelets and the like can be effectively reduced. Meanwhile, the phytic acid and cationic groups in the SBMA zwitterions are combined with bacterial cell membranes through electrostatic interaction, so that the bacterial structure is destroyed, and the antibacterial performance of the coating is further enhanced. In an in-vivo implantation experiment, the coating shows good biocompatibility.

This invention discloses a method for preparing and applying a photothermal nanoreagent targeting bacteria. This invention utilizes Ti3C2T... x Using MXene as a matrix, a photothermal nanoreagent MXene@PDA@Peptide (MPP) was constructed by sequentially modifying the surface of Ti3C2Tx MXene with polydopamine and grafting it with the peptide CAEKA, which has the function of recognizing bacteria. Under the recognition of the peptide CAEKA, the photothermal Ti3C2Tx MXene was targeted. x MXene nanosheets are guided to the surface of bacterial cells, achieving effective sterilization at a relatively low overall temperature of around 50°C (or achieving more efficient sterilization at the same overall temperature level, exhibiting superior sterilization performance compared to pure Ti3C2Tx MXene). This makes them particularly suitable for the preparation of therapeutic drugs for subcutaneous abscesses caused by methicillin-resistant Staphylococcus aureus (MRSA) infection.

This invention discloses a composite nanofiber membrane for immobilizing microorganisms to remediate diesel-contaminated water and its preparation method. Specifically, the composite nanofiber membrane is a thermoplastic polyurethane / nanohydroxyapatite composite nanofiber membrane, which not only possesses excellent biocompatibility and mechanical strength but is also non-toxic and harmless, offering considerable cost-effectiveness. Upon introduction into diesel-contaminated water, the composite nanofiber membrane first rapidly adsorbs diesel at the water / oil interface, preventing further diffusion and toxic effects on the aquatic environment. Subsequently, due to the additional adsorption sites provided by the nanoparticles on the membrane, a large number of free bacteria are adsorbed onto the membrane, enhancing the metabolic activity of bacterial cells and their resistance to the toxicity of stubborn compounds, while also strengthening the affinity between hydrophobic compounds and cells, thereby increasing the degradation rate of diesel hydrocarbons by microbial cells. This invention improves the remediation effect of diesel wastewater while expanding the application of composite nanofiber membranes in the field of water pollution treatment.

Antimicrobial eyewear features frames infused with or coated with antimicrobial additives, thereby offering continuous protection by actively destroying and inhibiting the growth of bacteria, fungi, parasites, and some viruses. The lenses may also have an antibacterial coating that destroys and inhibits bacterial growth. In some examples, the lenses are treated with a specialized strengthening liquid, wherein the liquid contains nano components that release heavy metal ions capable of invading the cell walls of bacteria, leading to bacterial elimination, destroying DNA molecules and proteases within the bacterial cells, denature viral proteins, break DNA chains, and result in cell death. Additionally, the strengthening liquid reduces dehydrogenase activity and interacts with various protein groups within the cell, thereby reducing the activity of these groups and effectively inhibiting the growth of E. coli and other bacteria on the lens surface.

Compositions of chitosan and one or more polyalcohols, such as mannitol and polyethylene glycol, and methods of use thereof, are provided. The disclosed compositions can deliver high concentrations of localized antimicrobials and metabolites to make infections more susceptible to antimicrobials. The disclosed compositions may be used to deliver biologically active agents to an infection at a site of trauma or surrounding an indwelling medical device, to treat an infection caused by persister bacterial cells, and to treat and / or prevent the formation of biofilm bacteria on an indwelling medical device or surrounding tissue.

The invention discloses a manganese-based layered hydroxide modified by gold nanoparticles and a preparation method thereof, and relates to the technical field of drug synthesis, and the key points of the technical scheme are as follows: the layered morphology of Mn-LDH is observed on the manganese-based layered hydroxide in a transmission electron microscope, and spherical AuNPs dispersed on the surface and edge of a laminate have a uniform particle size and a uniform particle size. EDS energy spectrum analysis finds that gold, manganese, magnesium, aluminum and carbon elements exist, the hydrated particle size is 312 nm, and the dispersity is good. The material can initiate an excellent photo-thermal effect under the irradiation of 808nm near-infrared light to generate active oxygen and hydroxyl radicals to destroy bacterial cell walls and cell membranes, and the antibacterial effect can reach 99%. In addition, the material can stimulate a local active immune function, promote T cell infiltration and realize active immune sterilization. The nano material shows a remarkable anti-infection effect in an in-vitro antibacterial experiment and an in-vivo osteomyelitis animal model experiment, and a new insight is provided for treating osteomyelitis caused by methicillin-resistant staphylococcus aureus.

The invention belongs to the field of food non-thermal sterilization, and relates to a self-assembled nanoparticle. The invention provides a self-assembled nano particle and a preparation method and application thereof, the self-assembled nano particle is combined with photodynamic force to realize photodynamic and physical multifunctional sterilization, and due to amphipathicity, modified chitosan has the capability of forming nano particles through self-assembly. Due to the introduction of phenolic ester, not only is the chitosan endowed with stronger photodynamic activity, but also the lipophilicity of the chitosan is improved, a hydrophobic chain part with a proper length can be effectively combined with bacterial cell membranes in a cuttage manner, efficient sterilization is realized through a physical effect and a photodynamic synergistic mechanism, and the antibacterial effect is remarkably improved. Meanwhile, the excellent AIE characteristic of the material is combined with efficient combination of bacteria, the material can be used for bacterial fluorescence imaging and development of efficient harmful bacteria monitoring means, and the material is completely prepared from food-borne raw materials and has excellent biocompatibility and high safety.

The invention discloses a novel liquid-holding particle type iodine-loaded antibacterial agent-ionic liquid gel iodine-loaded microsphere preparation method, which comprises the following steps: by taking polymethyl methacrylate as a network, a hydrophobic ionic liquid as a dispersion medium, methyl methacrylate as a monomer and azodiisobutyronitrile as an initiator, embedding molecular iodine in situ in the network, and preparing the novel liquid-holding particle type iodine-loaded antibacterial agent-ionic liquid gel iodine-loaded microsphere. The ionic liquid gel iodine-loaded microspheres are prepared. The ionic liquid cation imidazolyl group in the microbial agent can vertically act on a phospholipid bilayer on a bacterial cell membrane, and the hydrophobic end can destroy the complete structure of the cell membrane due to electrostatic interaction, so that substances outside the cell enter the cell or substances inside the cell leak out, and the cell is inactivated. Meanwhile, surface iodine in the ionic liquid gel iodine-loaded microspheres has an antibacterial effect, and the ionic liquid gel iodine-loaded microspheres and the surface iodine jointly inhibit growth of bacteria. The novel liquid-holding particle type iodine-loaded antibacterial agent-ionic liquid gel iodine-loaded microsphere is a novel solid-liquid composite polymer antibacterial material, and has the characteristics of simple preparation method, wide application range, excellent antibacterial performance and the like.

The current disclosure relates to the present disclosure is directed to an inducible promoter system for use in a thermophilic bacterial cell. In another aspect, the disclosure is directed to a thermophilic host cell comprising the inducible promoter system described herein. In a further aspect, the disclosure is directed to a method of controlling gene expression in a thermophilic bacterium.