42 results about "Escherichia" patented technology

The invention discloses a double-structural-domain recombinant human fibronectin, the sequence of which is shown as SEQ ID NO.3, and the double-structural-domain recombinant human fibronectin is formed by fusing a collagen binding structural domain and a cell binding structural domain of human fibronectin. The invention also provides a recombinant expression vector containing the protein coding gene and a host cell. The protein is efficiently expressed through eukaryotic cells and engineering bacteria escherichia coli, and a fermentation and purification process suitable for an industrial scale is established in a matched manner. Functional experiments prove that compared with commercially available fibronectin products with a single structural domain, the double-structural domain recombinant human chemical fibronectin disclosed by the invention can remarkably promote proliferation, migration, adhesion and differentiation activity of human skin fibroblasts, and has wide application potential in the fields of skin repair, skincare and biomedical materials.

The invention relates to the field of microorganisms, and discloses lactobacillus delbrueckii subsp. Bulgaricus, a fungicide and an additive containing the lactobacillus delbrueckii subsp. Bulgaricus, application of the fungicide and the additive and a pollution relieving method. The preservation number of the lactobacillus delbrueckii subsp. Bulgaricus is CCTCC (China Center For Type Culture Collection) NO: M 20251218, and the microbial agent comprises the lactobacillus delbrueckii subsp. Bulgaricus, the lactobacillus delbrueckii subsp. Bulgaricus and the microbial inoculum provided by the invention can be used for preparing food additives for preventing and / or relieving microplastic-induced body injury, reducing cadmium pollution of food and inhibiting food-borne enterobacter sakazakii, can provide a new scheme for infant food safety and health protection, and have relatively high application value.

The invention relates to methods for detecting the presence or absence of an enterohemorrhagic Escherichia coli (EHEC) in a sample and / or identifying said EHEC. The invention further relates to such methods comprising subjecting the sample or protein therein to mass spectrometry to obtain mass spectrometry data, comparing the obtained mass spectrometry data with reference data for at least one EHEC strain with a specific serotype and optionally one or more non-EHEC strains and detecting the presence or absence of the EHEC.

The invention discloses a recombinant plasmid, a recombinant strain, recombinant phycocyanin and application of the recombinant plasmid, the recombinant strain and the recombinant phycocyanin in mercury ion detection. The invention further discloses a dual-mode mercury ion biosensor based on the thermal-stability recombinant phycocyanin and a preparation method and application of the dual-mode mercury ion biosensor. The biosensor based on the recombinant phycocyanin has specific fluorescence response to trace mercury ions. The recombinant phycocyanin pigment binding rate is higher than that of all reported recombinant phycocyanin, great Stokes shift (288 nm) is shown, the mercury ion selectivity is high, the thermal stability is good, the prepared biosensor shows the characteristics of dual-mode output and ultra-sensitive sensing, the detection limit under a fluorescence-based mode is 2.94 nM, and the biosensor has good application prospects. And the detection limit is 1.87 nM in a colorimetric mode based on fluorescence. The protein-based sensor is obtained from escherichia coli, is simple and convenient, supports in-situ monitoring of a smart phone, and is low in cost, excellent in performance and free of any toxicity.

A transformed microorganism having an ability to produce a polyhydroxyalkanoate includes a polyhydroxyalkanoate synthase gene and a gene encoding a chaperone belonging to the ClpB family. The gene encoding the chaperone belonging to the ClpB family is a gene which has been introduced into the transformed microorganism or whose expression has been enhanced in the transformed microorganism. The chaperone belonging to the ClpB family may be derived from the genus Cupriavidus, Escherichia, or Saccharomyces. A polyhydroxyalkanoate can be produced by culturing the transformed microorganism.

Presented herein are systems and methods of selecting a cancer therapy in a cancer subject. A computing system may obtain, for a subject at risk of or diagnosed with cancer, a dataset comprising genome sequence reads of a biological sample obtained from the cancer subject. The computing system may identify, from the dataset, a first portion of the genome sequence reads corresponding to candidate Escherichia reads. The computing system may filter reads corresponding to a bacteriophage from the first portion of the genome sequence reads to generate a second portion of the genome sequence reads corresponding to true Escherichia reads. The computing system may determine, using the second portion of the genome sequence reads, a score indicating a number of true reads for Escherichia. The computing system may select, from a plurality of anti-cancer therapies, the anti-cancer therapy for the cancer subject based on the score.

The application discloses an Erwinia billingiae strain STSG25-LZ24 for promoting the germination of Thuja sutchuenensis seed and application thereof, the preservation number of the Erwinia billingiae strain STSG25-LZ24 disclosed by the application is CGMCC No.33386, and the 16S rRNA gene of the strain comprises a sequence as shown in SEQ ID NO.1. The strain can not only obviously promote the germination of Thuja sutchuenensis seed, but also has a good application prospect in the aspects of seed breeding, seed coating preparation and synthetic microbial population construction.

This invention belongs to the field of biomedical functional materials technology, and relates to an antibacterial and healing-promoting photosensitizer, its preparation method, and its uses. The photosensitizer is a defective MIL-88B@MIL-88C composite material, wherein defective MIL-88B serves as the substrate, and MIL-88C grows on the surface and / or in the pores of the defective MIL-88B. This photosensitizer significantly improves the photogenerated carrier separation efficiency, reduces impedance, enhances photocurrent response, and can efficiently generate reactive oxygen species under light irradiation. Antibacterial experiments show that it has excellent bactericidal effects against Staphylococcus aureus, Escherichia coli, and methicillin-resistant Staphylococcus aureus, and is not prone to inducing drug resistance. Simultaneously, this photosensitizer has good biocompatibility, promotes cell migration, and has significant hemostatic function. Animal models show that it can effectively accelerate the healing of skin defects.

The invention discloses escherichia coli capable of producing sakuranetin as well as a construction method and application of the escherichia coli. The escherichia coli capable of producing sakuranetin is introduced into a sakuranetin de novo synthesis path, a shikimic acid metabolism path is further reconstructed, 87.47 mg / L sakuranetin can be synthesized by fermentation in a shake flask only with glucose as a substrate, and the yield is the highest yield in currently reported escherichia coli. The invention further provides a method for producing sakuranetin through fermentation by taking the kelp extracting solution as the substrate, 57.4 mg / L sakuranetin can be synthesized by taking the kelp extracting solution as the substrate, so that high-value conversion of food industrial wastes can be effectively realized, and the production cost can be reduced.

The present invention relates to a microorganism belonging to the genus Escherichia sp. And having improved L-isoleucine production ability, and a method for producing L-isoleucine using the same, the microorganism having improved L-isoleucine production ability by weakening or deactivating the activity of acetohydroxy acid synthetases I and III or simultaneously enhancing the activity of acetohydroxy acid synthetase II, and the method for producing L-isoleucine by using the microorganism belonging to the genus Escherichia sp. Therefore, the biosynthetic pathway of the L-isoleucine is enhanced, the generation of byproducts is weakened, and compared with the parent strain, the production yield of the L-isoleucine can be improved.

The application discloses a double-domain recombinant humanized fibronectin, the sequence of which is shown as SEQ ID NO. 3, which is composed of the collagen binding domain and the cell binding domain of human fibronectin. The application also provides a recombinant expression vector and a host cell containing the gene coding the protein. The protein is efficiently expressed by eukaryotic cells and engineered Escherichia coli, and a fermentation and purification process suitable for industrial scale is established. Functional experiments prove that, compared with the single-domain commercial fibronectin product, the double-domain recombinant humanized fibronectin of the application can significantly promote the proliferation, migration, adhesion and differentiation activity of human skin fibroblasts, and has wide application potential in the fields of skin repair, beauty and skin care and biomedical materials.

The invention relates to a method for the fermentative production of recombinant proteins using fnr mutants of E. coli. Using these strains, increased amounts of recombinant protein in the culture medium can be achieved compared to the prior art.

The invention belongs to the technical field of biology, and particularly relates to a microbial marker combination for evaluating the quality of polygonatum sibiricum and application, the microbial marker combination comprises one or more of Aspergillus, Cercophora, Escherichia. Shigilla, Mycoplasm, Muribacaceae, Pseudomonas, Colletotrichum and Asaia, a method and an evaluation model for evaluating the quality of polygonatum sibiricum are established by utilizing the microbial marker combination, the sensitivity is 100.0%, the specificity is 72.7%, the AUC is 89.8%, and the evaluation model is used for evaluating the quality of polygonatum sibiricum. The method has high accuracy, specificity and sensitivity, and the evaluation method is economical, practical and easy and convenient to operate and can be widely applied to quality evaluation of rhizoma polygonati.

The application belongs to the technical field of biological medicine, and specifically provides application of a Prunus humilis Bge. extract in preparation of a preparation for regulating intestinal flora and / or improving intestinal barrier function. In particular, the application is in increasing abundance of Lachnospiraceae_NK4A136_group, Clostridia_UCG-014 and Ruminococcaceae; reducing abundance of Alistipes, Escherichia-Shigella and Bilophila; inhibiting ROS / OPN / αvβ3 / PI3K / Akt / NF-κB pathway; and reducing expression levels of TNF-α, IL-1β and IL-6 inflammatory factors. The new application of the Prunus humilis Bge. extract provides a new method for regulating intestinal flora and improving intestinal barrier function, and has important significance for deep development and utilization of Prunus humilis Bge. resources and industrial upgrading.

The invention discloses a covalent organic framework polymer capable of selectively inactivating staphylococcus aureus and application of the covalent organic framework polymer. The covalent organic framework polymer is obtained by polymerization of a ruthenium complex and heteropoly acid through aminal reaction, and the ruthenium complex is bis (hexafluorophosphate) tris (5, 5 '-bis (diethoxymethyl)-2, 2'-dipyridyl) ruthenium; the preparation method comprises the following steps: adding bis (hexafluorophosphate) tris (5, 5 '-bis (diethoxymethyl)-2, 2'-dipyridyl) ruthenium and Tris-V6O19 into an organic solvent, and carrying out solvothermal reaction, so as to obtain the covalent organic framework polymer. The m-RuV-COF prepared by the invention can selectively inactivate staphylococcus aureus at an extremely low concentration, and has a very small inactivation effect on escherichia coli. And a new effective strategy is provided for specifically solving the problem of staphylococcus aureus infection.

The present application discloses a spizizenii bacillus and application thereof in pear tree disease prevention and treatment. The spizizenii bacillus strain of the present application exhibits significant antagonistic activity to escherichia amylor and apple black rot pericarp pear variety, and can be used as a core bacterial resource for biological prevention and treatment of pear tree diseases, thereby providing a new bacterial resource and technical path for constructing a green prevention and control technology system of pear tree diseases.

The invention relates to the technical field of conjugational transfer engineering bacteria, in particular to a conjugational transfer donor bacterium as well as a construction method and application thereof, the conjugational transfer donor bacterium is Escherichia fergusonii EFmg-sy, is preserved in the China Center for Type Culture Collection (CCTCC), and has the preservation number of CCTCC NO: M2025310; the invention relates to a conjugational transfer donor bacterium which is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO: M20251730, or the conjugational transfer donor bacterium is Escherichia fergusonii EFmg-cj and is preserved in China Center for Type Culture Collection (CCTCC). The conjugational metastasis donor bacterium provided by the invention is stable in character, is simple to operate as a conjugational metastasis donor, and can be used for rapidly detecting and distinguishing the conjugational metastasis donor bacterium and a recipient bacterium receiving plasmids through a fluorescence detection method, so that the detection of the plasmid horizontal transfer capability of the Escherichia fjosenii is realized.

This invention relates to the field of fermentation technology, specifically disclosing a bacterial culture medium for high-density fermentation processes. Its purpose is to solve the problems of nutrient imbalance, byproduct inhibition, and insufficient dissolved oxygen in existing culture media during high-density bacterial fermentation, leading to low cell density and poor fermentation efficiency. The culture medium, by weight, comprises 15-30 parts carbon source, 8-18 parts nitrogen source, 0.5-2.5 parts complex trace elements, 0.3-1.2 parts growth promoter, and 0.1-0.8 parts inhibitor scavenger, diluted to 1000 parts with distilled water, with the pH adjusted to 6.5-7.8, and sterilized at 115-121℃ for 12-18 minutes before use. This invention achieves precise nutrient supply through the scientific ratio of each component, effectively removes inhibitory substances during fermentation, improves dissolved oxygen transfer efficiency, alleviates osmotic pressure stress, increases bacterial cell density by more than 30% compared to conventional culture media, shortens the fermentation cycle by 15%-25%, reduces production costs, and is suitable for high-density fermentation of various bacteria such as Escherichia coli, Bacillus, and Lactobacillus. It is applicable to industrial production scenarios such as biopharmaceuticals, food fermentation, and environmentally friendly preparations.

The invention discloses application of enzyme powder prepared from bamboo shoots to improvement of ulcerative colitis, which comprises the following steps: S1, pretreatment culture and grouping: 72 C57BL / 6J male mice are adaptively fed for one week and then are divided into 6 groups, each group comprises 12 male mice, and the number of the male mice is 12; the method comprises the following steps: sequentially naming a normal control group (NC), a model group (DSS), a sulfasalazine group (SASP, 300 mg / kg), a square bamboo shoot enzyme low-dose group (CUJ-L), a square bamboo shoot enzyme medium-dose group (CUJ-M) and a square bamboo shoot enzyme high-dose group (CUJ-H); experimental data of mice show that the square bamboo shoot enzyme treatment can significantly improve colon shortening and DAI, improve weight loss and reduce the colitis symptom factor level; besides, the chimonobambusa quadrangularis ferment intervention improves the diversity and the structure of intestinal bacteria, the composition of intestinal microbiota is remodeled, the abundance of Firmicite gate is improved, and the abundance of Proteobacteria and Verrucomicrobiota, including Escherichia-Shiga, Akkermansia and other species, is reduced, and the application of the chimonobambusa quadrangularis ferment intervention in the preparation of the chimonobambusa quadrangularis ferment intervention in the preparation of the chimonobambusa quadrangularis ferment intervention in the preparation of the chimonobambusa quadrangularis ferment intervention in the preparation of the chimonobambusa quadrangularis ferment is promoted. The intestinal metabolite and the intestinal flora have a close relationship; according to the method, 32 key metabolites are screened out through cecum metabolite analysis, and the key metabolites mainly relate to a Propionate metabolite metabolic pathway; the result shows that the square bamboo shoot enzyme has the potential of treating colitis.

Provided are a method for enhancing the immunogenicity of a recombinant protein antigen and use thereof. The method comprises conjugating a polysaccharide with a recombinant protein antigen to form a nano-scale protein antigen. The polysaccharide is selected from sodium hyaluronate, chitosan, glucan, fucoidan, and sodium alginate. The recombinant protein antigen is a protein antigen derived from a bacterium. The bacterium belongs to the genus of Staphylococcus, Neisseria, Klebsiella, Escherichia, Clostridium, Salmonella, Shigella, Pseudomonas, Acinetobacter, Bordetella, Enterococcus, Haemophilus, Mycobacterium, or Streptococcus. The method not only improves the immunogenicity of the recombinant protein antigen, but also overcomes the defects of competitive immunoregulation and poor immune enhancement effects caused by bacterial capsular polysaccharide modification. The method also has the advantages of high clinical application value, simple starting material acquisition, low cost, good process stability, ease in scale expansion, high safety, and the like.

The invention relates to methods for detecting the presence or absence of an enterohemorrhagic Escherichia coli (EHEC) in a sample and / or identifying said EHEC. The invention further relates to such methods comprising subjecting the sample or protein therein to mass spectrometry to obtain mass spectrometry data, comparing the obtained mass spectrometry data with reference data for at least one EHEC strain with a specific serotype and optionally one or more non-EHEC strains and detecting the presence or absence of the EHEC.

A microorganism useful as an expression host for γ-Glu-Val synthetase and a method for producing γ-Glu-Val-Gly using γ-Glu-Val synthetase expressed in the microorganism are provided. By using γ-Glu-Val synthetase expressed in a bacterium, for example Escherichia bacteria, modified so that the activity of a protein encoded by a ybdK gene (YBDK) is reduced as an expression host, γ-Glu-Val-Gly is produced from Glu, Val, and Gly as raw materials.

The application discloses an intestinal flora regulator containing unsaturated oligogalacturonic acid and application thereof, and an effective component of the intestinal flora regulator containing unsaturated oligogalacturonic acid comprises lemon unsaturated oligogalacturonic acid with a molecular weight of 80-85 kDa and an unsaturation degree of 7-10. The application finds that the intestinal flora regulator containing unsaturated oligogalacturonic acid has a regulating effect on intestinal flora by simulating an in-vivo environment in vitro and collecting intestinal microorganisms in human feces, including regulating the abundance, structure and diversity of intestinal flora, promoting the proliferation of beneficial bacteria such as bifidobacterium and lactobacillus, and inhibiting the proliferation of escherichia shigella and bacteroides, and can provide help for the development of intestinal disease treatment drugs.

The invention belongs to the technical field of degradable mulching films, and relates to a fully biodegradable mulching film as well as a preparation method and application thereof, and the fully biodegradable mulching film is prepared from oxidized starch, sodium carboxymethyl cellulose, polyvinyl alcohol and a plasticizer; the adhesive comprises the following components in parts by weight: 15-25 parts of oxidized starch, 20-25 parts of sodium carboxymethyl cellulose, 12-20 parts of polyvinyl alcohol and 30-40 parts of a plasticizer. The oxidized starch is prepared from cassava starch. The full-biodegradable mulching film is obtained by compounding polymer materials, the raw materials in the formula are low in price, can come from agricultural wastes, do not generate micro-plastics in the degradation process, and can be fully biodegraded, so that the problem of environmental pollution caused by the mulching film is solved, and the sustainable development of agriculture is promoted; the antibacterial activity on escherichia coli is realized; the preparation process is simple and safe, only water bath operation is needed, and operation such as professional production line and pressurization is not needed.

The invention relates to the technical field of biology, in particular to a cysticercosis cellulosae nano vaccine capable of remarkably enhancing host immune response and a preparation method and application of the cysticercosis cellulosae nano vaccine, and the cysticercosis cellulosae nano vaccine is designed to comprise amino acid sequences of a plurality of peptide fragments such as a taenia solium TSOL18 (T) sequence, a Mexican polypeptide vaccine epitope sequence (M), a CD40 epitope sequence (C) and a helicobacter pylori ferritin (F) sequence. And connecting through a linker molecule (GGGGS), and finally obtaining a full-length gene segment TMCF for short. The specific amino acid sequence and the connection sequence are shown as SEQ ID NO.1; a nucleotide sequence of TCMF is synthesized, enzyme cutting sites and a His tag sequence are added, and codon optimization is carried out. The synthetic TMCF and TSOL18 genes are respectively inserted into a pET-30 vector to construct a recombinant expression vector (pET-TSOL18 and pET-TSOL18-TMCF), and the recombinant expression vector is subjected to induced expression in escherichia coli. 1, and the nucleotide sequence is SEQ ID NO. 2; according to the invention, the humoral immune response level of the TSOL18 vaccine can be improved, and the immune response level of host cells can be obviously enhanced, so that the immune protection effect of the TSOL18 is further improved.

The application discloses antibacterial and deodorizing cat litter and a preparation method thereof, and steps are as follows: (1) a high-temperature-insensitive polymer waterproof layer is sprayed on the surface of a gas phase purification material to obtain the gas phase purification material with the polymer waterproof layer; the gas phase purification material is active particles loaded with permanganate, and the water content is 5-20 wt%; (2) a wetting agent is sprayed on the surface of the gas phase purification material, then powder is added, and the powder is adhered to the surface of the gas phase purification material through stirring; the spraying of the wetting agent and the adding of the powder are repeated until cat litter particles with a proper size are obtained; (3) the cat litter particles are first dried at low temperature, so that the water content of the outer layer is reduced to below 20 wt%; then the cat litter particles are heated at high temperature to destroy the polymer waterproof layer, and thus the antibacterial and deodorizing cat litter is prepared. The application has excellent antibacterial and deodorizing performance; the 1h contact sterilization rate of staphylococcus aureus and escherichia coli is as high as 99.99% or above; and the removal rate of ammonia gas and hydrogen sulfide and other odors is as high as 90% or above.

Provided herein are pre-protein signal peptides that direct secretion of expressed payload proteins to the periplasm in Escherichia bacteria and methods of their use in production of therapeutic and industrial commodity proteins. The disclosed pre-protein signal peptides may be used with any pay load protein to increase secretion thereof and therefore increase yield of the payload protein.

The invention relates to the technical field of visible light absorption spectrum detection, in particular to a three-dimensional fluorescence detection method and system for identifying escherichia coli in bile, and the method comprises the following steps: generating a light source through a light source generation module; adjusting the intensity of the light source by using a light splitting module, and dividing the light source into two paths of light so as to obtain sample detection light and a reference spectrum; acquiring a background spectrum and a bile detection spectrum by utilizing a sample detection module based on the sample detection light; according to the reference spectrum, the background spectrum and the bile detection spectrum, the upper computer uses a spectrum detection model to identify escherichia coli in the bile sample. Based on the detection principle of three-dimensional fluorescence, the intelligent degree and efficiency of detection of escherichia coli in bile can be improved, and the detection cost can be reduced.

The application discloses a phytolith-loaded nano-zinc oxide composite antibacterial material and a preparation method thereof. The following steps are included: step S1, phytolith and zinc nitrate hexahydrate are added into deionized water to form a phytolith-zinc nitrate hexahydrate mixed solution; step S2, sodium hydroxide solution is added dropwise into the mixed solution and stirred to react, so as to obtain a precursor phytolith-zinc hydroxide mixed solution; and step S3, the precursor phytolith-zinc hydroxide mixed solution is centrifuged, washed, dried and calcined under an air atmosphere to obtain the antibacterial material. In the application, the flaky phytolith mineral is used as a substrate, and the nano-zinc oxide is loaded. The two have opposite potentials, can be better loaded and adsorbed on the surface, the larger specific surface area and the existing mesoporous structure can improve the dispersity and loading rate of the nano-zinc oxide particles, so that the antibacterial performance of the material is improved. The material has very good antibacterial effect on escherichia coli and staphylococcus aureus, very low cytotoxicity and hemolysis rate, and excellent biocompatibility.

The application discloses a multiple digital PCR detection kit for diagnosing liver cirrhosis intestinal flora disorder. The kit is used for diagnosing pathogens including Soehngenella, Escherichia, Romboutsia, Clostridium, Fusobacterium and Mucispirillum in intestinal flora. By using the kit, specific strains in intestinal microflora in vivo can be detected to diagnose liver cirrhosis, thereby providing a new idea for diagnosis and treatment of liver cirrhosis.