90 results about "Escherichia" patented technology

The invention discloses a chitinase mutant obtained based on random mutation as well as a preparation method and application of the chitinase mutant. On the basis of an error-prone PCR (Polymerase Chain Reaction) technology, the mutant ChiA-7 (T165I / C253Y / A391T / N480S / T508S / K547T / I550V / N607T) of the chitinase ChiA of the bacillus thuringiensis is obtained by the invention. Compared with ChiA, the ChiA-7 has better stability, and the enzyme activity of the ChiA-7 is about 90% of the initial enzyme activity after the ChiA-7 is subjected to heat preservation for 3 hours at 40 DEG C in a phosphate buffer solution with the optimal catalysis condition of 40 DEG C and the pH value of 4.0, while the enzyme activity of the ChiA-7 is attenuated to 75% of the initial enzyme activity. The chiA-7 is connected with a carrier pET20b (+) and is transferred into escherichia coli Escherichia coli BL21 (DE3), and the extracellular enzyme activity of a recombinant bacterium reaches 10.23 U / mL, which is improved by 13.41% compared with the enzyme activity of a control bacterium for expressing chiA. The chitinase mutant obtained by the preparation method disclosed by the invention has good thermal stability and enzymatic activity, and can be applied to hydrolysis of chitin and inhibition of mould.

The invention discloses a double-structural-domain recombinant human fibronectin, the sequence of which is shown as SEQ ID NO.3, and the double-structural-domain recombinant human fibronectin is formed by fusing a collagen binding structural domain and a cell binding structural domain of human fibronectin. The invention also provides a recombinant expression vector containing the protein coding gene and a host cell. The protein is efficiently expressed through eukaryotic cells and engineering bacteria escherichia coli, and a fermentation and purification process suitable for an industrial scale is established in a matched manner. Functional experiments prove that compared with commercially available fibronectin products with a single structural domain, the double-structural domain recombinant human chemical fibronectin disclosed by the invention can remarkably promote proliferation, migration, adhesion and differentiation activity of human skin fibroblasts, and has wide application potential in the fields of skin repair, skincare and biomedical materials.

The invention discloses a coating material containing an agilawood component and a preparation method and application thereof, and belongs to the technical field of coating materials, a coating is composed of agilawood powder, paprika powder, natural glutinous rice glue, agilawood hydrolat and water, diatomite or beeswax emulsion can be selectively added, the oil content of the agilawood powder is larger than or equal to 15%, linalool is larger than or equal to 1.2 mg / g, and the agilawood powder is subjected to airflow crushing treatment; the glutinous rice glue is prepared by hydrolyzing starch, and the solid content is 25-35%; agilawood hydrolat is extracted through steam distillation, the concentration of agilawood alcohol is larger than or equal to 0.8 mg / mL, during preparation, raw materials are mixed and then stirred for 10-20 min at the high speed of 2500-3500 r / min at the temperature of 20-40 DEG C to form a stable system, the VOC content of the coating is smaller than 10 g / L, the coating meets the GB18582-2020 standard, the escherichia coli inhibition rate and the staphylococcus aureus inhibition rate are 95% and 98% respectively, the initial aroma intensity is 8.5 min (10 min), the coating is kept for 7.2 min after 12 months, the adhesive force is 0 grade, the pencil hardness is 2-4 H, and the coating is suitable for being applied to the field of medical care. The coating has the advantages of no less than 5000 times of washing resistance, no less than 91% of performance retention rate after 500 hours of ultraviolet aging, and suitableness for coating wall surfaces, furniture and handicrafts, and has the characteristics of aromatic physical therapy, antibiosis and environmental protection.

The invention discloses an Escherichia coli endogenous alcohol dehydrogenase EcYjgB mutant and application thereof. The mutant is obtained by performing any one of the following mutations (a) to (c) on Escherichia coli endogenous alcohol dehydrogenase EcYjgB as shown in SEQ ID NO.1: (a) mutating serine at the 200th site into arginine; (b) the serine at the 199th site is mutated into aspartic acid, and the serine at the 200th site is mutated into arginine; and (c) the serine at the 199th site is mutated into aspartic acid, the serine at the 200th site is mutated into arginine, and the asparagine at the 240th site is mutated into tryptophan. The mutant is used for efficiently catalyzing reduction of HMF or furfural to respectively synthesize BHMF and furfuryl alcohol, so that the purposes of improving the production efficiency, reducing the production cost and improving the environmental effect are achieved.

The invention discloses lactobacillus plantarum capable of efficiently producing acid and application of the lactobacillus plantarum in corn silage, and relates to the technical field of microorganisms. The invention provides lactobacillus plantarum Mei1101.3, the preservation number of the lactobacillus plantarum Mei1101.3 is CGMCC No.30332, the lactobacillus plantarum Mei1101.3 has the characteristics of high acid production rate, low pH value and excellent growth performance, the lactobacillus plantarum can be used as a special strain for corn silage and is added into corn silage, the pH value of the corn silage is reduced to 3.02 30 days after silage, and the lactobacillus plantarum Mei1101.3 can be used as a special strain for corn silage. The sensory quality is close to the initial state of the raw materials, the energy consumption in the ensiling process is only 374.34 kcal. Kg <-1 >, the quantity of saccharomycetes is reduced, no mould or escherichia coli is detected, and the health and safety meet the national standard.

A mixture includes a nickel-containing ore and / or concentrate thereof and an extraction composition including a fermentation broth obtained from an Aspergillus species (spp.), Acetobacter spp., Bacillus spp., Propionibacterium spp., Corynebacterium spp., Rhizopus spp., Clostridium spp., Fusobacterium spp., Pseudomonas spp., Bifidobacterium spp., Saccharomyces spp., Enterobacter spp., Escherichia spp., among others. The extraction composition is configured to selectively solubilize one or more components, such as a magnesium component from the nickel-containing ore and / or concentrate thereof. A method for extracting a metal from a nickel-containing ore and / or concentrate includes selectively solubilizing a magnesium component from the nickel-containing ore and / or concentrate thereof into an extraction composition including the fermentation broth. The method can include extracting a magnesium component from a nickel-containing ore and / or concentrate thereof into an extraction composition, separating the nickel-containing ore and / or concentrate thereof from an extracted magnesium component and broth, separating the broth from the extracted magnesium component, and repeating the extracting. The extracted magnesium component has a purity in a range from 10% to 90%.

The invention belongs to the field of gene engineering, and provides a mycoplasma ovipneumoniae multi-epitope fusion protein and preparation and application thereof.The inventor firstly successfully predicts antigen epitopes of three virulence genes and performs tandem expression on the predicated antigen epitopes to prepare a fusion protein antigen, and the fusion protein antigen is used for preparing the mycoplasma ovipneumoniae multi-epitope fusion protein. A secondary structure, a tertiary structure, physicochemical properties (including hydrophilicity, stability, PI value and the like), antigenicity, sensitization and the like of the fusion protein are predicted through biological online software, and the success rate of a test is increased; meanwhile, the fusion protein connected in series with the three virulence genes of the mycoplasma ovipneumoniae is prepared into a subunit vaccine, and a good immune protection effect is also achieved. The recombinant mycoplasma ovipneumoniae multi-epitope fusion protein prokaryotic expression plasmid pET28a < + >-EHP is successfully constructed, tandem expression of mycoplasma ovipneumoniae on an escherichia coli prokaryotic system is achieved, and an expression product can obtain a good immune protection effect.

The invention relates to the field of microorganisms, and discloses lactobacillus delbrueckii subsp. Bulgaricus, a fungicide and an additive containing the lactobacillus delbrueckii subsp. Bulgaricus, application of the fungicide and the additive and a pollution relieving method. The preservation number of the lactobacillus delbrueckii subsp. Bulgaricus is CCTCC (China Center For Type Culture Collection) NO: M 20251218, and the microbial agent comprises the lactobacillus delbrueckii subsp. Bulgaricus, the lactobacillus delbrueckii subsp. Bulgaricus and the microbial inoculum provided by the invention can be used for preparing food additives for preventing and / or relieving microplastic-induced body injury, reducing cadmium pollution of food and inhibiting food-borne enterobacter sakazakii, can provide a new scheme for infant food safety and health protection, and have relatively high application value.

The invention relates to methods for detecting the presence or absence of an enterohemorrhagic Escherichia coli (EHEC) in a sample and / or identifying said EHEC. The invention further relates to such methods comprising subjecting the sample or protein therein to mass spectrometry to obtain mass spectrometry data, comparing the obtained mass spectrometry data with reference data for at least one EHEC strain with a specific serotype and optionally one or more non-EHEC strains and detecting the presence or absence of the EHEC.

The invention discloses a recombinant plasmid, a recombinant strain, recombinant phycocyanin and application of the recombinant plasmid, the recombinant strain and the recombinant phycocyanin in mercury ion detection. The invention further discloses a dual-mode mercury ion biosensor based on the thermal-stability recombinant phycocyanin and a preparation method and application of the dual-mode mercury ion biosensor. The biosensor based on the recombinant phycocyanin has specific fluorescence response to trace mercury ions. The recombinant phycocyanin pigment binding rate is higher than that of all reported recombinant phycocyanin, great Stokes shift (288 nm) is shown, the mercury ion selectivity is high, the thermal stability is good, the prepared biosensor shows the characteristics of dual-mode output and ultra-sensitive sensing, the detection limit under a fluorescence-based mode is 2.94 nM, and the biosensor has good application prospects. And the detection limit is 1.87 nM in a colorimetric mode based on fluorescence. The protein-based sensor is obtained from escherichia coli, is simple and convenient, supports in-situ monitoring of a smart phone, and is low in cost, excellent in performance and free of any toxicity.

A transformed microorganism having an ability to produce a polyhydroxyalkanoate includes a polyhydroxyalkanoate synthase gene and a gene encoding a chaperone belonging to the ClpB family. The gene encoding the chaperone belonging to the ClpB family is a gene which has been introduced into the transformed microorganism or whose expression has been enhanced in the transformed microorganism. The chaperone belonging to the ClpB family may be derived from the genus Cupriavidus, Escherichia, or Saccharomyces. A polyhydroxyalkanoate can be produced by culturing the transformed microorganism.

The invention discloses a method for improving L-histidine production efficiency of recombinant escherichia coli based on a substrate channel strategy and application, and belongs to the technical field of biology. The invention provides a recombinant escherichia coli, the recombinant escherichia coli is fermented in a 5L fermentation tank at the temperature of 37 DEG C, the pH value of 7.0-7.2 and the dissolved oxygen of 25-35% for 36 hours, the yield of L-histidine reaches 57.8 g / L, the average production intensity is 1.6 g / L / h, and the conversion rate is 0.23 g / g. Therefore, the L-histidine produced by fermenting the recombinant escherichia coli has the advantages of short fermentation period, high production efficiency and easiness in operation.

Presented herein are systems and methods of selecting a cancer therapy in a cancer subject. A computing system may obtain, for a subject at risk of or diagnosed with cancer, a dataset comprising genome sequence reads of a biological sample obtained from the cancer subject. The computing system may identify, from the dataset, a first portion of the genome sequence reads corresponding to candidate Escherichia reads. The computing system may filter reads corresponding to a bacteriophage from the first portion of the genome sequence reads to generate a second portion of the genome sequence reads corresponding to true Escherichia reads. The computing system may determine, using the second portion of the genome sequence reads, a score indicating a number of true reads for Escherichia. The computing system may select, from a plurality of anti-cancer therapies, the anti-cancer therapy for the cancer subject based on the score.

The application discloses an Erwinia billingiae strain STSG25-LZ24 for promoting the germination of Thuja sutchuenensis seed and application thereof, the preservation number of the Erwinia billingiae strain STSG25-LZ24 disclosed by the application is CGMCC No.33386, and the 16S rRNA gene of the strain comprises a sequence as shown in SEQ ID NO.1. The strain can not only obviously promote the germination of Thuja sutchuenensis seed, but also has a good application prospect in the aspects of seed breeding, seed coating preparation and synthetic microbial population construction.

The invention discloses a recombinant strain for producing farnesene as well as a construction method and application thereof. The construction method comprises the following steps: preparing a pFII plasmid containing farnesene synthase, farnesyl pyrophosphate synthase and isopentene diphosphate isomerase; taking the pFII plasmid as a template, and carrying out PCR (Polymerase Chain Reaction) amplification by utilizing the primer 1 and the primer 2 to obtain a fragment 1; taking the pFII plasmid as a template, and carrying out PCR (Polymerase Chain Reaction) amplification by utilizing a primer 3 and a primer 4 to obtain a fragment 2; taking the pFII plasmid as a template, and carrying out PCR (Polymerase Chain Reaction) amplification by utilizing a primer 5 and a primer 6 to obtain a fragment 3; carrying out seamless connection on the fragment 1, the fragment 2 and the fragment 3 to obtain a plasmid 1; introducing a plasmid 1, a plasmid 2 and a plasmid 3 into escherichia coli to obtain a recombinant strain for producing farnesene; the farnesene yield of the recombinant strain 1 constructed by the invention is 3.3 g / L within 96 hours, and technical support and data reference are provided for large-scale preparation of biological fuel farnesene by a biological method.

The invention relates to the technical field of microorganisms, in particular to giant spheroidin Escherichia and application of the giant spheroidin Escherichia in promoting intestinal development of piglets. The Megasphaera elsdenii provided by the invention is preserved in Guangdong Microbial Culture Collection Center on May 22, 2025, the preservation number is GDMCC No: 66369, and the Megasphaera elsdenii can be used for promoting the development of intestinal tracts and intestinal mucosa of piglets and retarding growth retardation caused by weaning stress. In addition, the in-vitro pure culture supernatant of the giant spheroidin can also improve the viability of the jejunum stem cells and promote the proliferation of the epithelial cells of the pig intestinal tract.

The invention discloses an acid-resistant element derived from Escherichia coli and application of the acid-resistant element. The acid-resistant elements are respectively HdeB and GlsA, and the acid-resistant elements are constructed on an expression vector and are introduced into corynebacterium glutamicum to obtain an acid-resistant engineering strain. A research result shows that the acid-resistant element has a good effect on improving the acid resistance of corynebacterium glutamicum in an acid environment such as a condition that the pH value is 5.5, and the application potential of the acid-resistant element in fermentation strain modification is proved.

This invention belongs to the field of biomedical functional materials technology, and relates to an antibacterial and healing-promoting photosensitizer, its preparation method, and its uses. The photosensitizer is a defective MIL-88B@MIL-88C composite material, wherein defective MIL-88B serves as the substrate, and MIL-88C grows on the surface and / or in the pores of the defective MIL-88B. This photosensitizer significantly improves the photogenerated carrier separation efficiency, reduces impedance, enhances photocurrent response, and can efficiently generate reactive oxygen species under light irradiation. Antibacterial experiments show that it has excellent bactericidal effects against Staphylococcus aureus, Escherichia coli, and methicillin-resistant Staphylococcus aureus, and is not prone to inducing drug resistance. Simultaneously, this photosensitizer has good biocompatibility, promotes cell migration, and has significant hemostatic function. Animal models show that it can effectively accelerate the healing of skin defects.

The invention belongs to the technical field of bioinformatics, and discloses a transcription factor and transcription factor target prediction method and system, and the method comprises the following steps: collecting information data of Escherichia coli, building an Escherichia coli metabolism regulation knowledge graph, and building a heterograph by using the Escherichia coli metabolism regulation knowledge graph and an ESM-2 model; encoding the heterogeneous graph by using an encoding algorithm, and constructing an FM-HGN model according to the encoded heterogeneous graph by using a graph attention network as a trunk; and predicting the transcription factor by using an FM-HGN model, and identifying a transcription factor target according to a transcription factor prediction result in combination with regulation and control information of the transcription factor. According to the method, the key transcriptional regulation relationship of the escherichia coli can be directly mined from the existing knowledge base, and a solid foundation is provided for predicting and analyzing a transcriptional regulation network in the future.

The invention discloses escherichia coli capable of producing sakuranetin as well as a construction method and application of the escherichia coli. The escherichia coli capable of producing sakuranetin is introduced into a sakuranetin de novo synthesis path, a shikimic acid metabolism path is further reconstructed, 87.47 mg / L sakuranetin can be synthesized by fermentation in a shake flask only with glucose as a substrate, and the yield is the highest yield in currently reported escherichia coli. The invention further provides a method for producing sakuranetin through fermentation by taking the kelp extracting solution as the substrate, 57.4 mg / L sakuranetin can be synthesized by taking the kelp extracting solution as the substrate, so that high-value conversion of food industrial wastes can be effectively realized, and the production cost can be reduced.

The invention discloses a bifunctional protein tag for improving thermal stability and solubility of recombinant protein, a product based on the bifunctional protein tag and application of the bifunctional protein tag, and belongs to the technical field of protein engineering. The amino acid sequence of the bifunctional tag is shown as SEQ ID NO: 1, the bifunctional tag has the similarity of 70%-95% or above, and the amino acid sequence variation mainly exists but is not limited to a secondary structure position. The bifunctional tag can be used at an N terminal or a C terminal of a target protein, and is suitable for expression and purification of a fusion protein in a prokaryotic expression system. The hydrophobic region of the natural protein is transformed through rational design, so that the technical problems that the existing protein tag is large in molecular weight and easy to shield the active structural domain of the target protein, and the residual non-natural amino acid after the tag is removed possibly destroys the correct folding of the protein are solved; the solubility and the thermal stability of the fusion protein in an escherichia coli expression system are remarkably improved, and the method is suitable for functional expression and industrial application of inclusion body protein.

The invention relates to a reagent card box for detecting spirochetes of severe fever with thrombocytopenia syndrome virus, Erhi and Lyme disease, relates to the technical field of molecular biology detection, and comprises a primer probe composition for detecting spirochetes of severe fever with thrombocytopenia syndrome virus, Erhi and Lyme disease, and a kit for detecting spirochetes of severe fever with thrombocytopenia syndrome virus, Erhi and Lyme disease. The primer and the probe for detecting the severe fever with thrombocytopenia syndrome virus comprise a forward primer as shown in SEQ ID NO: 1, a reverse primer as shown in SEQ ID NO: 2 and a probe as shown in SEQ ID NO: 9, the primer and the probe used for Escherichia coli detection comprise a forward primer shown in SEQ ID NO: 3, a reverse primer shown in SEQ ID NO: 4 and a probe shown in SEQ ID NO: 10. The primer and the probe for lyme disease spirochete detection comprise a forward primer of SEQ ID NO: 5, a reverse primer of SEQ ID NO: 6 and a probe of SEQ ID NO: 11. The reagent card box disclosed by the invention adopts a POCT (Point-of-Care Testing) technology to carry out multiple real-time fluorescent PCR amplification detection on nucleic acids of the severe fever with thrombocytopenia syndrome virus, the Escherichia body and the Lyme disease spirochetes, and has timeliness, closeness, accuracy and convenience.

The utility model provides water supply treatment equipment applied to dispersive emergency suspended matters. Comprising an ultrafiltration system, an adsorption filter material system and a disinfection system which are connected in sequence, the ultrafiltration system comprises a water inlet pipeline, an ultrafiltration membrane, an ultrafiltration membrane pool, a water production pump, a middle water tank, a fan and a backwashing pump which are connected in sequence, and the adsorption filter material system comprises an adsorption filter material water inlet pump, an adsorption filter material tank and an adsorption filter material backwashing pump which are connected in sequence. The water supply treatment equipment disclosed by the utility model is highly integrated, low in investment cost, small in occupied area, short in production period and rapid in response to a water supply treatment site. Meanwhile, the operation cost is low, the daily operation and maintenance management requirement is low, the intelligent degree is high, and unattended operation can be truly achieved. The equipment adopts an advanced ultrafiltration, filter material adsorption and sodium hypochlorite disinfection process, and is supplemented with a sterilization and disinfection function, so that suspended matters, metal elements such as iron, manganese and zinc and escherichia coli in raw water can be effectively removed, and the quality of discharged water is ensured.

The invention relates to the technical field of microorganisms. The invention provides a strain of Gamaleyavius phage RKP-VP21052, the strain of Gamaleyavius phage RKP-VP21052 is preserved in the General Microbiological Center of the China Committee for Culture Collection of Microorganisms on September 27, 2024, and the preservation number of the Gamaleyavius phage RKP-VP21052 is CGMCC No.46192. The invention also provides a preparation method of the Gamaleyavius phage. The invention also provides a phage composition. The invention also provides an application of the Gamaleyavius bacteriophage RKP-VP21052 or the bacteriophage composition in the preparation of a product for preventing and / or treating livestock and poultry diseases caused by escherichia coli. The invention further provides the application of the Gamaleyavius bacteriophage RKP-VP21052 or the bacteriophage composition. The invention also provides a bacteriophage pharmaceutical preparation, wherein the effective components of the bacteriophage pharmaceutical preparation comprise the Gamaleyavius bacteriophage RKP-VP21052 or the bacteriophage composition disclosed by the invention. The invention also provides a drinking water additive, a feed additive, a disinfectant, a cleaning agent and the like. The Gamaleyavius bacteriophage RKP-VP21052 provided by the invention has a very good advantage in the aspect of reducing the concentration of escherichia coli, does not cause damage to probiotics, and is relatively high in titer and good in prevention and treatment effect.

The invention relates to metabonomic characterization of microorganisms, in particular to a method for identifying cell types of cells in a sample, comprising: culturing the sample in a growth medium comprising nicotinamide to obtain a cultured growth medium; analyzing the cultured growth medium by chemical analysis; and identifying the cell type as at least one of the genus Escherichia, the genus Klebsiella, the genus Pseudomonas, the genus Enterococcus, the genus Staphylococcus or the genus Streptococcus when the cultured growth medium contains a higher concentration of nicotinate than the growth medium; wherein the growth culture medium comprises the following components in a buffer culture medium: 0.02 to 2 g / l of D-glucose, 0.0001 to 0.1 g / l of nicotinamide, 0.0001 to 0.1 g / l of pyridoxin.HCl, 0.02 to 1 g / l of hypoxanthine, 0.2 to 5 g / l of L-arginine, 0.2 to 5 g / l of L-threonine, 0.1 to 5 g / l of L-histidine, 0.02 to 2 g / l of spermine and catalase. According to the method, identification and toxin sensitivity detection can be completed within about half of time compared with that of an existing method.

The invention discloses a construction method of a crisprcas9 visual model based on fluorescent Escherichia coli, a red fluorescent protein gene is introduced into an Escherichia coli BL21 genome to construct a mcherry Escherichia coli model, and an easy-to-use biological model capable of verifying a Crisper / cas9 knockout effect and fluorescent Escherichia coli which can be quantified, is easy to observe, is high in sensitivity and is low in background are obtained.

The invention discloses an intestinal microbial marker combination and system for detecting fish liver injury and application, and belongs to the technical field of molecular detection of fish liver injury. The invention relates to an enteric microbial marker combination, which is characterized in that the enteric microbial marker combination comprises the following components: cetyl, pseudomonas and Escherichia-Shigella, and the enteric microbial marker combination comprises the following components: bacillus, pseudomonas and Escherichia-Shigella, and the enteric microbial marker combination comprises the following components: bacillus, pseudomonas and Escherichia-Shigella. By utilizing the intestinal microbial marker combination, non-invasive diagnosis of liver injury can be realized on the premise of not invading a fish body, the accuracy is high, missing detection is avoided, and particularly, the condition that liver injury is misjudged to cause excessive treatment is avoided. A judgment basis is provided for breeding environment regulation and control and precise disease prevention and treatment, the breeding survival rate of the pelteobagrus fulvidraco is increased, and the breeding benefit is improved.

The application belongs to the technical field of new materials of multilayer structure, and discloses a kind of high-temperature and high-humidity resistant oil-resistant food preservation composite nanometer membrane and preparation method thereof.The method comprises the following steps: (1) polylactic acid solution is electrospun to form electrospun fiber membrane; (2) on one side of the obtained electrospun membrane, antimicrobial A-containing konjac gum solution is electrostatically sprayed; (3) on the side where electrostatic spraying is completed, polylactic acid solution is electrospun again; (4) the outer side of the obtained product of step (3) is electrostatically sprayed with modified konjac gum and chitosan composite solution containing antimicrobial B.The food preservation composite nanometer membrane obtained by the application has excellent high-temperature and high-humidity resistance, and the tensile strength loss rate is less than 7% and the elongation at break loss rate is less than 11% under the conditions of 45 DEG C temperature and 95% humidity for 24 hours; meanwhile, it also has excellent antibacterial rate, and the antibacterial rate on escherichia coli can reach 99.9%.

The invention discloses a compound preparation for regulating vaginal microecology as well as preparation and application thereof, and relates to the field of biological medicines. The composite preparation is composed of a fusion polypeptide and a monoclonal antibody according to the ratio of 1: 2 (w / w), the fusion polypeptide comprises a cell adhesion domain, an enzymolysis linking peptide and a quorum sensing inhibition domain, the amino acid sequence is shown as SEQ ID NO: 1, and the protease hydrolysis resistance is improved by more than 4 times after cyclization oxidation; the monoclonal antibody takes specific surface protein of lactobacillus inertus as a target spot, and can induce the strain to generate H2O2 phenotype conversion. An in-vitro experiment shows that the degradation rate of the composite preparation on a vaginal bacterial biofilm reaches 92.3 + / -2.4%, the ratio of lactobacillus acidophilus is remarkably increased to 63.8 + / -3.3%, and the pH is effectively adjusted to 4.3; animal experiments show that compared with commercially available metronidazole gel, the compound preparation can more remarkably promote proliferation of lactobacillus, inhibit escherichia coli and staphylococcus aureus and restore vaginal micro-ecological balance, and a new scheme is provided for treatment of vaginal micro-ecological imbalance related diseases.

The present invention relates to a microorganism belonging to the genus Escherichia sp. And having improved L-isoleucine production ability, and a method for producing L-isoleucine using the same, the microorganism having improved L-isoleucine production ability by weakening or deactivating the activity of acetohydroxy acid synthetases I and III or simultaneously enhancing the activity of acetohydroxy acid synthetase II, and the method for producing L-isoleucine by using the microorganism belonging to the genus Escherichia sp. Therefore, the biosynthetic pathway of the L-isoleucine is enhanced, the generation of byproducts is weakened, and compared with the parent strain, the production yield of the L-isoleucine can be improved.