134 results about "L-Isoleucine" patented technology

L-threonine or L-isoleucine is produced by culturing a bacterium which belongs to the genus Escherichia and has an ability to produce L-threonine or L-isoleucine, and wherein expression of a threonine operon is directed by its native promoter, and from which at least a leader sequence and an attenuator are deleted, in a medium and collecting the L-threonine or L-isoleucine from the medium.

Personal care composition comprising at least one hair growth regulating compound selected from the group consisting of glyceryl dilaurate, apigenin, tetrahydrocurcumin, oleanolic acid, azelaic acid, sulforaphane, canavanine, pyridoxal 5-phosphate, phytic acid, tannic acid, grape seed extract, NG-nitro-L-arginine-methyl ester, benzamidine, sodium butyrate, betulinic acid, polyornithine, polyarginine, fisetin, jasmonates, methyl-jasmonate, cis-jasmone, caffeic acid phenethyl ester, delphinidin, ethyl abietate, esculetin, sorbic acid methyl ester, canaline, N-formyl-methionine, N-formyl-alanine, taurine, palmitoyl carnitine, undecanol, undecylenic acid, rutin, fusidic acid, phenyl pyruvic acid, L-isoleucine, phenyl glycine, silibinin, silymarin, L-ascorbic acid-6-palmitate, N-undecylenoyl-L-phenylalanine, and salts, derivatives and mixtures of any of the foregoing; and a dermatologically-acceptable carrier.

The invention discloses a dark melolonthidae sex pheromone and a sex attractant. The sex pheromone comprises the following components: L-isoleucine methyl ester, (R)-(-)-linalool and 1-cyclohexane amino acid with the weight ratio of 2-9:1:0-1. The sex attractant comprises the dark melolonthidae sex pheromone and organic solvents; and the weight ratio of the sum of all components in the dark Melolonthidae sex pheromone and the organic solvents is 1:2.2-100. The dark melolonthidae sex pheromone is beneficial to protecting natural enemies, benefiting and eliminating pests, giving full play to integrated control power and improving the control effect when being used for forecasting insects and preventing dark melolonthidae pests by using a trapping method and an interference mating method; and in addition, the application of chemical pesticides is avoided so as to reduce the pollution, protect the environment and ensure remarkable economic benefits, social benefits and ecological benefits. The invention is suitable for the control of the dark Melolonthidae pests in farmlands.

The present invention provides a fatigue-preventive (brain fatigue) agent or a fatigue-recovering agent for the central nervous system, which contains a branched-chain amino acid, such as L-valine, L-leucine and L-isoleucine, and/or a 2-aminobicyclo-[2,2,1]-heptane-2-carboxylic acid. In addition to the application as injection and transfusion, these can be prepared as solid-state modes to be taken, such as tablets, pellets and powder medicine by adding an appropriate vehicle such as starch and lactose thereto. Moreover, these can be prepared as various beverages such as so-called health drinks and sports drinks, or as food items for fatigue-recovering and the fatigue-prevention in the central nervous system.

The invention discloses a composition for relieving physical fatigue and enhancing an immunologic function, a preparation method thereof and application thereof. The composite comprises 10 to 100 weight parts of guarana extract, 100 to 800 weight parts of taurine, 1 to 10 weight parts of L-lysine hydrochloride, 1 to 10 weight parts of L-isoleucine, 0.1 to 5 weight parts of L-leucine, 0.2 to 5 weight parts of L-valine, 1 to 15 weight parts of L-aspartate, 0.01 to 0.3 weight part of L-tryptophan, 0.01 to 0.3 weight part of L-phenylalanine, 0.01 to 0.3 weight part of L-threonine, 0.01 to 0.3 weight part of L-methionine, 10 to 100 weight parts of L-alanine, 10 to 100 weight parts of glycine, 1 to 15 weight parts of tea polyphenol and 1 to 20 weight parts of American ginseng extract.

The invention relates to a clean producing craft for extracting L-isoleucine from fermentation liquor in the field of biology chemical technology. It comprises: preparing for the L-isoleucine, regenerating ion exchange column, cycle using the regenerated ammonia sulfate waste liquid; the L-isoleucine preparing course comprises: heat filtering the fermentation liquor, adjusting acid, flocculation filtering, using strong acidic hydrion exchange resin column to do negative pressure to the elutriation liquid, washing it after saturation adsorption to expel the foreign substance, adding ammonia with ammonium waste water to do elutriation, using strong acidic hydrion exchange resin column to do negative pressure to the L-isoleucine again, washing it after saturation adsorption to expel the foreign substance, adding ammonia to do elutriation to obtain the L-isoleucine product. The ion exchange column after elutriating uses sulphuric acid to regenerate; part of the ammonia sulfate waste fluid is used in the bulk of the ferment customs agency; the other part is as the eluent of first ion exchange after adding ammonia, which forms a closed circuit.

An anti-diarrhea complete formula feed for piglets comprises, by weight, 55-56 parts of extruded corn, 6-10 parts of broken rice, 13-18 parts of enzymatic soybean meal, 9.5-10 parts of low-protein whey powder containing 3% of CP, 3-5 parts of Peruvian steam fishmeal, 0.8-1 part of L-lysine hydrochloride, 0.13-0.18 parts of DL-methionine, 0.09-0.13 parts of L-tryptophan, 0.35-0.4 parts of L-threonine, 0.25-0.3 parts of L-valine, 0.1-0.15 parts of L-isoleucine, 0.18-0.2 parts of L-cystine, 0.08-0.12 parts of choline chloride, 0.83-0.88 parts of calcium hydrogen phosphate, 0.95-1 part of calcium carbonate, 0.8-0.88 parts of salt, 0.04 parts of a vitamin premix, 0.4 parts of a trace elements premix and 0.03-0.05 parts of other auxiliary additives. A feed nutrient ration is reduced, and reasonable burdening is carried out by treating an amount required by an NRC2012 piglet as a standard, so the digestible nutrient content of the feed is improved, thereby the feed resource is reduced, the nutrient draining amount of an animal is reduced, the intestinal digestion burden is reduced, and the diarrhea is mitigated.

The invention belongs to the field of gene engineering, and discloses a mutant of a PET (polyethylene terephthalate) degrading enzyme and the application of the PET degrading enzyme. The mutant of thePET degrading enzyme is obtained by point mutation at a substrate binding site position of IsPETase, and comprises an IsPETase mutant M1, wherein arginine at the site 89 of IsPETase is mutated into alanine, an IsPETase mutant M2 where arginine at the site 89 of IsPETase is mutated into alanine and leucine at the site 117 is mutated into phenylalanine, and an IsPETase mutant M3 where arginine at the site 89 of IsPETase is mutated into alanine and leucine at the site 208 is mutated into phenylalanine. An experiment shows that compared with wild IsPETase, the three PETase mutants have the advantages that the hydrolysis activity is obviously improved, and PET can be efficiently degraded at a medium temperature (30 DEG C).

A powdered composition which provides improved muscle growth and helps reduce fatigue, comprising a combination of edible nutritional whey protein, citrulline malate, and at least one branched chain amino acids. The branched chain amino acids are L-isoleucine, L-leucine, and L-valine. The concentration of nutritional protein present in the composition ranges from about 10% by weight to about 96% by weight, and may be about 20% by weight to about 90% by weight. The composition may contain additional ingredients such as polypeptides, a hyper-immune milk protein concentrate, amino acids, antioxidants and various flavorings and sweeteners. A method of use of the composition is also disclosed.

The invention discloses a health-care food composition with a physical fatigue relieving function and an immunity enhancing function. A formula of the health-care food composition comprises the following components in parts by weight: 12 parts of guarana extract, 3 parts of root of American ginseng extract, 83 parts of taurine, 5 parts of L-isoleucine, 1.25 parts of L-leucine, 2.5 parts of L-valine, 0.02 part of L-tryptophan, 0.02 part of L-phenylalanine, 0.02 part of L-threonine, 0.02 part of L-methionine, 1 part of L-lysine hydrochloride, 1 part of L-aspartic acid, 12 parts of glycine, 12 parts of L-alanine and 80 parts of white kidney bean extract. A preparation containing the health-care food composition disclosed by the invention can be a beverage, a capsule, a tablet, a chewable tablet, a granular formulation, an oral solution, a soft capsule, paste, flowing liquid and semi-flowing liquid. A health-care product disclosed by the invention adopts a low-sugar formula and the intake of human bodies to sugar can be reduced; meanwhile, the absorption of people on starch substances in daily diet can be inhibited, so that risks of diseases including blood glucose, blood pressure, obesity and the like are reduced.

The invention discloses an extraction method of L-isoleucine; the technique process of the invention includes the following steps: obtaining zymotic fluid containing the L-isoleucine by zymotechnics; carrying out filtration on the zymotic fluid after adding a neutral flocculating agent to remove thalli; adding active carbon into filtrate to carry out decoloring; the size of the liquid is condensed to 9 to 11 percent of the original size by heating and vacuum condensing; the condensed liquid is crystallized after being cooled to 24.5 to 25.5 DEG C; then the crude product is obtained by centrifugalizing; the crude product is dissolved into liquor of 3.5 percent and heated up to between 58 to 62 DEG C, then is decolored, filtered, condensed, crystallized and separated to obtain the secondary crude product; the operations are carried out again on the secondary crude product; only a hyperfiltration process is added when filtering and finally the wet finished product is obtained after crystallization. The extraction method has the advantages of greatly reducing the drain amount of waste water as the working procedure of ion exchange is replaced by only adopting the method of condensing crystallization; furthermore, the extraction absorption rate is also remarkably improved.

The invention discloses a composition with functions of relieving physical fatigue and improving memory as well as a preparation method and application thereof. The composition contains the following components in parts by weight: 5-60 parts of guarana extract, 60-600 parts of taurine, 0.3-8 parts of L-isoleucine, 0.1-2 parts of L-leucine, 0.2-5 parts of L-valine, 5-60 parts of L-alanine, 5-60 parts of glycine, 0.3-8 parts of L-lysine hydrochloride and 1-20 parts of American ginseng extract.

A novel aldolase is described. 4-hydroxy-3-methyl-2-keto-pentanoic acid, which is useful as an intermediate in the synthesis of 4-hydroxy-L-isoleucine, may be synthesized from acetaldehyde and α-ketobutyric acid using a novel aldolase, which is derived from the genus Arthrobacter.

The invention relates to a genetic engineering bacterium for producing L-isoleucine and an application thereof, which belong to the field of metabolic engineering. The genetic engineering bacterium isobtained by overexpressing an acetohydroxyacidsynthase encoding gene ilvBN<M>, a threonine dehydratase encoding gene ilvA, and a threonine operon thrABC releasing feedback inhibition of L-isoleucine,the acetohydroxyacidsynthase encoded by ilvBN<M> releases the feedback inhibition of L-isoleucine, and its activity is not significantly lower than that of the wild-type ilvBN-encoded acetohydroxyacidsynthase; The L-isoleucine genetic engineering bacterium of the invention has no nutritional deficiency, rapid growth, short fermentation cycle, high yield and high conversion rate, and after 26-36hfermentation, the concentration of L-isoleucine in a fermentation broth reaches 42.5-51.6g / L.

The present invention discloses a genetically engineered bacterium of corynebacterium glutamicum producing L-isoleucine. ddh gene encoding diaminopimelate dehydrogenase is knocked out in a genome of astarting strain. A nucleotide sequence of the ddh gene is shown as SEQ ID No:2; a lysC operon encoding an aspartokinase is inserted, the lysC operon contains a lysC mutant gene, a 5'-end promoter anda 3' end termination thereof, and a nucleotide sequence of the lysC mutant gene is shown as SEQ ID No:1; and besides, the starting strain is C. glutamicum H5 delta argG delta alaT::ilvC with a preservation number of CCTCC NO:M2016609. The present invention also discloses a preparation method and an application of the genetically engineered bacterium. The production of the L-isoleucine by the genetically engineered bacterium is increased by 5%, synthesis of a by-product lysine of a metabolic branch is reduced by 45.24%, production cost is lowered, and the genetically engineered bacterium has abroad industrial application prospect.

The invention belongs to the technical field of base acid production, and discloses a method for extracting L-isoleucine through whole membrane technology. The method comprises the following steps: step 1) filtering through a micro-filtration membrane and removing impurities through flocculation; step 2) filtering through an ultra-filtration membrane, step 3) concentrating through a reverse osmosis membrane; step 4) concentrating and crystallizing. The membrane technology is combined with the flocculation agent to remove the impurities, the process is simple and feasible, and the yield and the purity of the product are greatly improved.

The invention relates to the field of cell culture medium, and in particular to a non-serum non-animal-origin-additive insect cell culture medium. The medium comprises mainly the following components: basic culture medium, glucose, inorganic salt, vitamins, L-arginine, L-agedoite, L-glycocoll, L-histidine, L-isoleucine, L-leucine, L-lysine, L-methionine, L-threonine, L-tryptophan, L-valine, L-proline, L-glutamine, yeast extracts, recombulin, malic acid, allomaleic acid, cholesterol, linoleic acid, granulesten, putrescine, glutathione, glycerol and fructosan. The culture medium can promote the growth of insect cell and is suitable for the large scale breeding of insect cell and the expression of recombination protein.

The present invention is a novel nutritional product composition formulated to be used as medical foods for managing COPD. The formula comprises clary sage root extract, L-Leucine, L-Isoleucine, L-Valine and L-Glutamine. The most active chemicals that import antioxidants proprieties are Tanshinones. The composition enhances the activity of Phase 2 enzymes that deactivate and react to oxygen species and many toxins present in tobacco smoke. Preparation of highly enriched Tanshinones improved the lung function in patients with chronic asthmatic bronchitis.

The invention provides a composition for enhancing muscle power and a preparation method thereof. Functional components of the composition comprise the following components in parts by weight: 10-500 parts of soybean oligopeptides, 10-100 parts of L-leucine, 10-100 parts of L-isoleucine, 10-100 parts of L-valine, and 2-10 parts of oligosaccharides; and the composition is obtained by mixing the components. The composition employs soybean oligopeptides as a functional nutrient with extremely low sensitization and extremely high absorptivity, specific amino acids and oligosaccharides are compounded with mutual synergistic effects, so that the composition has substantial effects for alleviating or eliminating sport fatigue and mental fatigue, and good mouthfeel without any side effects; the preparation method is simple and is suitable for needs of large scale industrial production.

The invention provides a protein-free serum-free medium suitable for diploid cell culture and a preparing method thereof. The medium contains calcium chloride, magnesium sulfate, potassium chloride, sodium dihydrogen phosphate, L-arginine hydrochloride, L-asparaginate, L-cystine dihydrochloride, L-histidine hydrochloride, L-leucine, L-isoleucine and the like. The medium can meet the requirement of the growth or maintenance stage of diploid cells and prevents contact with exogenous viruses possibly existing in protein and serum to reduce potential safety hazards; as the components are simple and definite, standard products can be purchased in the market, the difference between different product batches is small, and the step of purifying down-stream products is simplified; a scientific basis is provided for large-scale standard production. In addition, the protein-free serum-free medium can be applied in the growth or maintenance stage of the diploid cells.