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Corynebacterium glutamicum fermentation culture medium and corynebacterium glutamicum fermentation culture method for producing L-isoleucine

A technology of Corynebacterium isoleucine glutamicum and fermentation medium, which is applied in the field of microbial fermentation, can solve the problems of low production efficiency of L-isoleucine and the like, and achieves the improvement of sugar-acid conversion rate, yield and production cycle. short effect

Active Publication Date: 2017-05-24
WUHAN GRAND HOYO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a kind of Corynebacterium glutamicum fermentation medium and culture method for producing L-isoleucine for the current situation that the production efficiency of L-isoleucine is low at present, adopt this fermentation medium and The cultivation method can greatly increase the sugar-acid conversion rate, thereby improving production efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] A kind of L-isoleucine-producing Corynebacterium glutamicum fermentation medium, it is made up of basal medium and growth factor, and the content of each component in fermentation medium is:

[0013] Basal medium: corn steep liquor 15ml / L, glucose 240g / L, urea 25g / L, dipotassium hydrogen phosphate 0.4g / L, magnesium sulfate 0.6g / L, vitamin B1 0.3mg / L, ferrous sulfate 0.015mg / L , corn oil 1ml / L, silk peptide powder 3g / L, defoamer 34ml / L.

[0014] Growth factors: betaine 0.3mg / L, choline 0.5g / L, vitamin B6 0.3mg / L.

[0015] After mixing, sterilize at 121°C for 25 minutes.

[0016] Corynebacterium glutamicum fermentation culture method: Corynebacterium glutamicum is inoculated in above-mentioned fermentation medium, and bacterial liquid volume accounts for 10% of fermentation medium volume, and ammoniacal liquor adjusts pH to 6.8, and dissolved oxygen is controlled at 30% (weight) , fermented for 26h; then the dissolved oxygen was reduced to 20%, and in the fermented liqu...

Embodiment 2

[0018] A kind of L-isoleucine-producing Corynebacterium glutamicum fermentation medium, it is made up of basal medium and growth factor, and the content of each component in fermentation medium is:

[0019] Corn steep liquor 15ml / L, glucose 300g / L, urea 20g / L, dipotassium hydrogen phosphate 0.4g / L, magnesium sulfate 0.6g / L, vitamin B1 0.4mg / L, ferrous sulfate 0.03mg / L, corn oil 5ml / L, silk peptide powder 2g / L, defoamer 50ml / L, choline 0.8g / L, betaine 0.4mg / L, vitamin B6 0.2mg / L.

[0020] Corynebacterium glutamicum fermentation culture method: Inoculate Corynebacterium glutamicum into the fermentation medium, the volume of the bacterial liquid accounts for 5% of the volume of the fermentation medium, adjust the pH to 6.5 with ammonia water, control the dissolved oxygen at 30%, and ferment for 25 hours; Then the dissolved oxygen was reduced to 15%, and 80% glucose solution was added to the fermentation broth to control the residual sugar to be 3%, and the fermentation was conti...

Embodiment 3

[0022] A kind of L-isoleucine-producing Corynebacterium glutamicum fermentation medium, it is made up of basal medium and growth factor, and the content of each component in fermentation medium is:

[0023] Corn steep liquor 25ml / L, glucose 240g / L, urea 25g / L, dipotassium hydrogen phosphate 0.8g / L, magnesium sulfate 0.8g / L, vitamin B1 0.2mg / L, ferrous sulfate 0.015mg / L, corn oil 1ml / L, silk peptide powder 4g / L, defoamer 30ml / L, choline 0.3g / L, betaine 0.5mg / L, vitamin B6 0.1mg / L.

[0024] Corynebacterium glutamicum fermentation culture method: Inoculate Corynebacterium glutamicum into the fermentation medium, the volume of the bacterial liquid accounts for 20% of the volume of the fermentation medium, adjust the pH to 7 with ammonia water, control the dissolved oxygen at 50%, and ferment for 30 hours; Then the dissolved oxygen was reduced to 25%, and 50% glucose solution was added to the fermentation broth to control the residual sugar to be 4%, and the fermentation was conti...

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Abstract

The invention discloses a corynebacterium glutamicum fermentation culture medium for producing L-isoleucine. The fermentation culture medium comprises a basic culture medium and a growth factor, wherein the growth factor is prepared from choline, betaine and vitamin B6; the content of the choline in the fermentation culture medium is 0.2-1g / L, the content of the betaine in the fermentation culture medium is 0.25-0.5mg / L, and the content of vitamin B6 in the fermentation culture medium is 0.05-0.3mg / L. The fermentation culture medium provides the growth factor for corynebacterium glutamicum, maintains osmotic pressure inside and outside the cells of the corynebacterium glutamicum to be balanced, and promotes the anabolism of amino acid, thus improving the yield and sugar-acid conversion rate of the L-isoleucine.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a Corynebacterium glutamicum fermentation medium and a cultivation method for producing L-isoleucine. Background technique [0002] In recent years, with the deepening of research on the application of L-isoleucine in medicine, health care, food processing and feed industry, the market demand for L-isoleucine is constantly growing. Although L-isoleucine has been industrially produced, the current output still cannot meet the needs. At present, there is a large demand gap for L-isoleucine in my country, and the annual demand is increasing year by year. The production of L-isoleucine in my country faces many problems such as low acid production, low sugar-acid conversion rate, and low extraction rate. The acid production level of L-isoleucine in my country is 25-30g / L, the extraction rate is 65-70%, the highest sugar-acid conversion rate in China is 18-20%, and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/06C12R1/15
CPCC12P13/06C12N1/20C12N2500/05C12N2500/30C12N2500/34C12N2500/38C12N2500/76
Inventor 梅雪臣王炯万坤宋盟军邢盼盼苏海霞李敬刘爱福
Owner WUHAN GRAND HOYO
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