379 results about "Basal medium" patented technology

The invention relates to organoid culture. The invention provides a human epidermal organoid differential medium, a method for constructing an epidermal model and application of the human epidermal organoid differential medium, the human epidermal organoid differential medium comprises a basic medium component and an additive, and the additive comprises lipid. According to the method, the organoid is directly used for constructing the epidermal model, so that the problem of few cell sources is solved; a lipid component is added into the epidermal organ differentiation culture medium, so that the epidermal organ can enhance the barrier function of the cuticle, further differentiation of the epidermal organ can be effectively promoted by utilizing the epidermal organ differentiation culture medium, and a human epidermal model with a basal layer, a spinous layer, a granular layer and a cuticle which are completely morphologically differentiated is obtained. And the operation is simple, large-batch production is easy to realize, and a reliable tool is provided for in-vitro observation, interference of skin disease generation, injury repair and other life activities.

The present invention discloses a complete medium for culturing DF-1 cells, including DMEM basal medium 13.7g / L, insulin, non-essential amino acids, transferrin, glutathione, ascorbyl phosphate, sodium selenite, ethanolamine, hydrocortisone, and newborn calf serum. Wherein non-essential amino acids include glycine, alanine, proline, serine, and asparagine. A complete medium for culturing DF-1 cells provided by the present invention can well promote the proliferation of DF-1 cells, can achieve the same proliferation effect as high-concentration fetal bovine serum, and is suitable for the adherent culture of DF-1 cells; using low-concentration newborn calf serum, it is possible to greatly avoid the batch differences, unstable culture results, cytotoxicity, and high risk of carrying pathogens brought by the culture medium with high-concentration serum, narrow application range, expensive fetal bovine serum, and high culture cost; the components are simple, the culture medium preparation process is short in time consumption, the culture efficiency is high, the repeatability is good, and it is suitable for batch production.

The invention discloses a sheep formation state pluripotent stem cell culture medium and application thereof. The invention belongs to the technical field of biology, and particularly relates to a sheep formation state pluripotent stem cell culture medium and application thereof. The composition for culturing the sheep formation state pluripotent stem cells, disclosed by the invention, contains an N2B27 basic culture medium and cell factors, wherein the cell factors contain CHIR99021, IWR1, A419259, a recovery hub IL6, a recovery hub sIL6R, a recovery hub Activin A and a recovery hub FGF2 (Fibroblast Growth Factor 2). The culture medium prepared from the composition can obtain stable sfPSCs, and can be applied to construction of sheep gene editing model animals.

The invention belongs to the field of stem cell biology, and relates to pedigree specific differentiation of human pluripotent or embryonic stem cells, in particular to a mesenchymal stem cell induced differentiation culture medium and application thereof. The mesenchymal stem cell induced differentiation culture medium is used for directly inducing and differentiating human induced pluripotent stem cells / or embryonic stem cells into mesenchymal stem cells, the culture medium comprises a mesenchymal stem cell basic culture medium and an additive, and the additive comprises the following components: 2-10 [mu] M of a TGF-beta inhibitor, 2-10 [mu] M of a GSK3 beta inhibitor and 5-10 [mu] M of a Rock inhibitor. By adopting the induced differentiation culture medium provided by the invention, human induced pluripotent stem cells / or embryonic stem cells can be directly induced and differentiated into mesenchymal stem cells, and the obtained mesenchymal stem cells have the advantages of infinite sources, stable quality, high homogeneity and the like; and the human induced pluripotent stem cells / or embryonic stem cells can be directly and directionally differentiated into the mesenchymal stem cells in a relatively short time, so that the culture time is shortened, and the production cost is reduced.

The invention belongs to the technical field of assisted reproduction, and particularly relates to a device and a method for gradually adjusting the glucose concentration of an in-vitro embryo culture solution. The device comprises at least two miniature liquid storage bins, wherein each miniature liquid storage bin is filled with a high-concentration glucose solution in advance; the slow release channel is respectively connected with each micro liquid storage bin and the culture solution main container; the culture solution main container is used for accommodating an embryo and a basal culture medium; the control unit is used for sequentially opening the release ports of the miniature liquid storage bins, so that the high-concentration glucose solution sequentially flows into the culture solution main container through the slow release channels. The technical means of staged closed-loop micro sugar control is introduced into the structure and the using method of the device, so that the accurate adjustment of the sugar concentration of the in-vitro embryo culture solution is realized, the requirements of embryos in different development stages can be effectively met, and the embryo culture quality and the subsequent development potential are remarkably improved.

The invention relates to the technical field of cell culture. The invention provides a mesenchymal stem cell culture medium without animal-derived components and application of the mesenchymal stem cell culture medium. The culture medium comprises a basic culture medium and an additive, the additives comprise FGF-2, IGF-1, glutamine, folic acid, serotonin, cholesterol, arachidonic acid, an HEPES buffer solution, sodium bicarbonate, SCF, TGF-beta, PDGF, ascorbic acid and transferrin. The mesenchymal stem cell culture medium disclosed by the invention is completely free of animal-derived components, and meets the requirements of FDA / EMA clinical-grade cell therapy products. The culture medium can support high-density amplification of mesenchymal stem cells, the activity of passage cells is effectively improved, and the amplification time is shortened. The method has remarkable significance in improving the amplification efficiency of the mesenchymal stem cells and promoting and applying factory growth.

The invention relates to an N2B27 culture medium for inducing generation of bidirectional pluripotent stem cells, one of LY2090314, AS1842856 or CHIR99021 is added into an N2B27 basal culture medium, and the concentration of the CHIR99021 is 10 mu M. The invention also relates to a preparation method of the N2B27 culture medium. The weight of the LY2090314 is 10 nM, and the weight of the AS1842856 is 0.6 [mu] M. The invention also provides an application of the culture medium in induced production of bidirectional pluripotent stem cells. According to the invention, a novel stem cell culture condition for promoting co-expression of genes OCT4 and CDX2 by adding small molecules LY2090314 (LY), AS1842856 (AS) and CHIR99021 to inhibit signal channels of FOXO1 and GSK3 is screened out. The culture conditions are simple and convenient, the application range is wide, the cultured cells keep the characteristics of 16-32 cell stages of the embryos, and the development characteristics of the embryos can be efficiently reproduced. Under the condition of not depending on transcription factors, the screened bidirectional pluripotent stem cells (BPSCs) can promote efficient generation of TSC cells and establish a TSC cell line through independent differentiation of a serum culture medium or induced differentiation of a TSC culture medium. The obstacles between early embryo pedigree are broken, and the research on early embryo development is promoted.

Techniques for generating and utilizing adult hepatocyte organoids are described. An example method includes generating an organoid by culturing hepatocytes obtained from an adult donor in a hepatocyte culture medium. The hepatocyte culture medium includes a basal medium and 5 μM of a TGF-β inhibitor.

The invention discloses a culture medium composition for culturing GDT cells and a culture method, and belongs to the field of biological medicine. Aiming at the problems of low in-vitro amplification efficiency and poor function of the existing GDT cells, the invention provides the culture medium composition for culturing the GDT cells, the culture medium composition comprises an induction culture medium and a proliferation culture medium, the induction culture medium comprises a basic culture medium and an activating agent, and the activating agent comprises IL-7 and paclitaxel; the proliferation culture medium comprises a basic culture medium, IL-18 and a metabolism regulator. An STAT5 pathway is activated through IL-7, and amplification of the Vdelta2 subgroup is specifically promoted; on the other hand, through the cooperation of the IL-7 and the IL-18, the IL-18 activates the expression of the NKG2D, and the IL-18 and the IL-7 jointly enhance the cytotoxicity; efficient amplification and function enhancement of the GDT cells are achieved; the killing rate of PD-L1 + tumor cells is larger than 80%, the motility rate after cryopreservation recovery is larger than 90%, the function maintenance rate is larger than 85%, and the cell line is suitable for tumor immunotherapy, infectious disease treatment and cell drug development.

The present invention discloses a buffer for tissue separation and / or preservation and its application, and a method for culturing tumor organoids, and relates to the field of organoid culture. The buffer is used to preserve tumor tissue samples, which are of liquid origin; the buffer includes: a basal culture medium and specific additive factors, and the specific additive factors include: superoxide dismutase, catalase, penicillin, and streptomycin; based on the volume of the basal culture medium, the concentration of superoxide dismutase is 1 to 100 μg / mL, the concentration of catalase is 50 to 500 μg / mL, the concentration of penicillin is 50 to 200 U / mL, and the concentration of streptomycin is 50 to 200 μg / mL. The tissue preservation solution of the present invention is used for the transportation and protection of liquid-derived tumor tissue samples, and has a high tumor cell activity retention rate. It is used to construct tumor organoids with a high success rate and a large number, which can meet the needs of clinical detection and disease research.

The invention provides an osteogenic induced differentiation kit, a culture medium and a preparation method of the culture medium, and particularly relates to the technical field of stem cells. The kit comprises a DMEM (dulbecco's modified eagle medium) basal culture medium, fetal calf serum, dexamethasone, vitamin C, beta-glycerol phosphate, mesenchymal stem cell osteogenesis induced supernatant, alizarin red S staining solution and acetic acid. According to the kit provided by the invention, the problems of slow calcium nodule formation, small calcium nodule quantity and small calcium nodule in the osteogenic induced differentiation of the umbilical cord mesenchymal stem cells in the prior art are solved, so that the calcium nodule formation of the umbilical cord mesenchymal stem cells is effectively promoted, meanwhile, the calcium nodule quantity can be increased, and the osteogenic induced differentiation period is shortened.

The invention belongs to the field of organoid culture, and particularly relates to a mixed type gastric cancer organoid culture medium and a culture method and application thereof. Wherein the culture medium comprises a basic culture medium and a specific additive factor; the culture method comprises the following specific steps: treating mixed gastric cancer tissues with tissue digestive juice to obtain mixed gastric cancer primary cells, and culturing with a mixed gastric cancer organ culture medium to obtain mixed gastric cancer organs. The mixed type organ-like form corresponding to the mixed type gastric cancer tissue can be cultured by using the mixed type gastric cancer organ-like culture medium disclosed by the invention. By means of methods such as organoid passage and cryopreservation technologies, the culture success rate of the mixed type gastric cancer organoid is high, sufficient mixed type organoids can be obtained within limited time, an accurate tumor model is provided for individualized tumor therapy, and valuable samples are preserved for scientific research.

The invention discloses a culture system, culture method and application of gastrointestinal tumor chemotherapy drug-resistant organoid, the culture system comprises: a basic culture medium, which is a DMEM / F12 culture medium, and is additionally added with a special growth promoting factor obtained from a natural extract; the multiple growth factors comprise an epidermal growth factor (EGF), a fibroblast growth factor (FGF), a bone morphogenetic protein (BMP) and a vascular endothelial growth factor receptor inhibitor (VEGFRi), and the concentration of each growth factor is accurately screened and optimized; the extracellular matrix is Matrigel modified by physical and chemical methods; the specific additive comprises an antioxidant N-acetylcysteine (NAC). According to the culture system, the culture method and the application of the gastrointestinal tumor chemotherapy drug-resistant organoid, the culture system which is closer to a real environment in vivo is constructed by optimizing basic culture medium components, setting various growth factors and specific additive concentrations and adopting modified Matrigel as an extracellular matrix.

The invention belongs to the technical field of biomedicine, and relates to a method for increasing the yield of pancreas islet organs differentiated by pluripotent stem cells and improving the stability. The method comprises the following steps: (1) inoculating pluripotent stem cells, and culturing by adopting a basic culture medium containing Y-27632; (2) changing the liquid, and continuously culturing by adopting a basic culture medium; (3) culturing for 1 day by using an S1D1 culture medium, and then culturing for 2-3 days by using an S1D2 culture medium; (4) changing the liquid, and culturing for 2-3 days by adopting the S2 culture medium; (5) changing the liquid, and culturing for 1-2 days by adopting an S3 culture medium; (6) changing the liquid, and culturing for 4-7 days by adopting a culture medium S4; (7) changing the liquid, and culturing for 3-10 days by adopting an S5 culture medium; and (8) carrying out cell sphere repolymerization, and culturing for 3-21 days by using an S6 culture medium to obtain the pancreas islet organ. The present invention increases the yield and differentiation efficiency at each differentiation stage and increases the scale yield at the cellular level.

Platelet lysate compositions and cell culture media compositions for maintaining and / or growing mammalian cells, such as mammalian endothelial cells (ECs) and mammalian endothelial progenitor cells (EPCs), in particular human ECs (huECs) and human EPCs (huEPCs), such as primary huECs and primary huEPCs, are provided. The cell culture media compositions contain a basal medium, a platelet lysate and, optionally, one or more exogenously added growth factors. Also provided are methods for making and using such cell culture media compositions to grow and / or maintain ECs and EPCs, including huECs and huEPCs, as well as cell culture vessels, dishes, plates, and / or flasks pretreated with the cell culture media compositions.

The present invention discloses a low - serum medium and its application in the preparation of cultured meat from Larimichthys crocea cells, which is also applicable to other marine or freshwater fish, belonging to the field of bioengineering technology. The low - serum medium comprises a basal medium, fetal bovine serum accounting for 30 - 80 μL / mL, and exogenous additives. The exogenous additives include human serum albumin, ascorbic acid, basic fibroblast growth factor polypeptide, human holo - transferrin, lysophosphatidic acid, and human recombinant IGF - 1. Based on the DMEM / F12 medium, the low - serum medium provided by the present invention adds six substances that promote cell growth, reduces the dependence of satellite cells on fetal bovine serum, effectively reduces the usage amount of fetal bovine serum, and saves the culture cost. Compared with the general - type medium (DMEM high - glucose, adding 10% FBS), the low - serum medium provided by the present invention has an equivalent effect on the maintenance and proliferation ability of cell stem cells.

The present invention relates to the field of cell culture, and particularly to a serum-free induction adipogenic differentiation medium for fish stem cells and its application. It is composed of a basal medium and a combination of cell culture cofactors; the basal medium is one of DMEM and DMEM / F12 medium; the combination of cell culture cofactors consists of oleic acid, soybean lecithin, vitamin C phosphate, β-cyclodextrin, transferrin, saikosaponin A, bakuchiol, dehydroabietic acid, and phenyl lactic acid. By adding the combination of cell culture cofactors, the present invention replaces the serum in the traditional adipogenic induction differentiation medium for preadipocytes and the core component dexamethasone IBMX in the traditional adipogenic differentiation "cocktail", and obtains a medium suitable for serum-free induction adipogenic differentiation of fish stem cells, which not only solves the bottleneck problems of the prior art, but also provides reliable technical support for the large-scale production of cell-cultured fish meat.

The invention provides a culture medium composition and a method for preparing mesenchymal stem cells. The culture medium combination comprises a culture medium A, a culture medium B and a differential culture medium, the culture medium A comprises a basic culture medium and a first small molecule compound combination, the culture medium B comprises a basic culture medium and a second small molecule compound combination, and the differential culture medium comprises a basic culture medium and a third small molecule compound combination. According to the culture medium and the method, the MSC can be prepared through ES or iPSC, and the culture medium and the method can be widely applied to the fields of regenerative medicine, tissue engineering, immunotherapy and the like.

The invention discloses an application of a composition of L-arginine and L-histidine in improving the yield of N-acetylglucosamine produced by microbial fermentation. The invention also discloses a culture medium for fermentation production of N-acetylglucosamine. The culture medium comprises a basic culture medium and a fed-batch culture medium, wherein the fed culture medium is prepared from the following components: 600 to 800 g / L of glucose, 4 to 6 g / L of monopotassium phosphate, 80 to 120 g / L of glycerol, 0.8 to 1.5 g / L of L-arginine and 0.8 to 1.5 g / L of L-histidine. According to the method, L-arginine and L-histidine with specific concentrations are added in the microbial fermentation process for producing N-acetylglucosamine, so that the yield of N-acetylglucosamine can be remarkably increased.

The invention relates to the field of cell culture, in particular to a serum-free induced adipogenic differentiation culture medium for fish stem cells and application of the serum-free induced adipogenic differentiation culture medium. The culture medium consists of a basic culture medium and a cell culture auxiliary factor combination, the basic culture medium is one of a DMEM (Dulbecco's Modified Eagle Medium) and a DMEM / F12 culture medium; the cell culture auxiliary factor combination is prepared from oleic acid, soya bean lecithin, vitamin C phosphate, beta cyclodextrin, transferrin, saikoside A, psoralen flavanone methyl ether, dehydroabietic acid and phenyllactic acid. The culture medium suitable for serum-free induced adipogenic differentiation of fish stem cells is obtained by adding a cell culture auxiliary factor combination to replace serum in a traditional adipose precursor cell adipogenic induced differentiation culture medium and dexamethasone IBMX serving as a core component of traditional adipogenic differentiation cocktail, the bottleneck problem in the prior art is solved, and the culture medium is suitable for serum-free induced adipogenic differentiation of fish stem cells. And reliable technical support is also provided for large-scale production of cell culture fish meat.

The invention relates to a culture medium for separating and culturing stem cells in tissues and application of the culture medium. The culture medium is a basic culture medium containing genipin, plasmalogen and cell factors. The brand-new culture medium is designed, genipin, plasmalogen and cell factors are added into the basic culture medium and are integrally and synergistically matched, stem cells in tissues (such as nasal mucosa tissues) can be quickly activated, the adherence and migration speed of the stem cells is remarkably increased, proliferation of the stem cells is promoted, and cell activity is effectively maintained; the activation and separation efficiency of the stem cells is obviously improved.

A bifidobacterium enrichment agent and a use thereof in high-density fermentation. The components of the bifidobacterium enrichment agent include potassium carbonate, arginine, methionine, serine, vitamin C, vitamin B1, vitamin B5, thymine, and calcium pantothenate. The bifidobacterium enrichment agent is added to a basal medium for high-density fermentation, so that the proliferation performance of bifidobacteria can be significantly improved, and the production efficiency can be improved, providing the possibility of preparing a highly active bifidobacterium fermentation liquor, and also providing support for the subsequent preparation and application of bacterial agents.

The present invention discloses a culture medium for culturing a liver cancer microtumor model and a culture method thereof. The present invention relates to the field of biotechnology, and provides a culture medium for culturing a liver cancer microtumor model, which is composed of three antibacterial and antifungal agents, HEPES, GlutaMax, human recombinant protein EGF, FGF‑10, HGF, IL‑2, IL‑15, R‑spondin 1, forskolin, gastrin, A83‑01, N‑acetyl‑L‑cysteine, nicotinamide, N2 additive, B27 and basal culture medium. The liver cancer microtumor model obtained by the method of the present invention can accurately reflect the various characteristics of the original lesion of the patient, and is a good scientific research experimental model and preclinical experimental model in the field of precise diagnosis and treatment of tumors. It can be foreseen that this culture method has broad application prospects in the research and clinical diagnosis and treatment of liver cancer.

The invention provides a culture method of fibroblast stem cells and application of the fibroblast stem cells in facial beautification, and relates to the technical field of cell biology. The culture medium provided by the invention comprises a basic culture medium, a growth factor, an immunomodulatory factor, an antioxidant protective agent and amino acid, the growth factors comprise bFGF, TGF-beta and EGF (Epidermal Growth Factor); the immunoregulation factors comprise IL-6 (Interleukin-6) and IL-10 (Interleukin-10); the antioxidant protective agent comprises vitamin C and glutathione. Through scientific combination of the growth factor, the immune factor and the antioxidant, the culture medium and the culture method significantly improve the proliferation efficiency, the passage frequency and the functional stability of the fibroblast stem cells, and efficient and safe cell products are obtained.

The invention belongs to the technical field of cell culture, and particularly relates to a serum-free mesenchymal stem cell culture medium and a preparation method thereof. The serum-free mesenchymal stem cell culture medium provided by the invention is prepared by taking a DMEM (Dulbecco Modified Eagle Medium) / F12 basal culture medium as a basis and adding supplementary factors, namely yeast hydrolysate, insulin, albumin, dexamethasone, taurine, salidroside, soy isoflavone, baicalein, human platelet lysate, momordica saponin and hyperoside. According to the serum-free mesenchymal stem cell culture medium provided by the invention, the normal form of cells can be maintained without supplementing serum, rapid proliferation is realized, and the biological characteristics of the mesenchymal stem cells are maintained.

The invention relates to an NK cell culture medium and an NK cell culture method, and relates to the technical field of cell culture.The NK cell culture medium comprises a basic culture medium and an additive, and the additive is prepared from, by concentration, 200-400 IU / L of cytokines, 3-6 mmol / L of nutritional supplements, 1-2 mmol / L of metabolism regulators, 90-110 mg / L of vitamin C, 150-300 micrograms / mL of plant polysaccharides, 1-2 micrograms / mL of 1, 2-propylene glycol, 1-2 micrograms / L of 1, 3-propylene glycol and 1, 3-propylene glycol. The medicine is prepared from 50-100 [mu] mol / L of 1, 3-dicaffeoylquinic acid, 48-52 [mu] g / mL of nano-scale hydroxyapatite, 14-16 [mu] mol / L of a signal channel regulator, 5-10 IU / L of heparin sodium and 10-80 [mu] g / mL of astragaloside IV-liposome. The culture medium and the culture method can efficiently amplify the NK cells and maintain the activity and the functional stability of the NK cells.

The invention relates to the technical field of cell biology, in particular to a culture medium for inducing neural stem cells and application thereof.The culture medium for inducing the neural stem cells comprises a neural ectoderm induction culture medium and a neural stem cell induction culture medium; the neural ectoderm induction culture medium is prepared from a basic culture medium, an N-2 additive, a B-27 additive, an inhibitor SB431542 and an inhibitor Dorsomorphin, the neural stem cell induction culture medium is prepared from a basic culture medium, an N-2 additive and a B-27 additive, the basic culture medium is composed of a DMEM / F12 culture medium, a Neurobase culture medium, GlutaMax and non-essential amino acid NEAA. The problems that neural stem cells obtained through an existing single-layer adherent induction method based on small molecules or growth factors are uneven in differentiation and low in purity are solved.

The invention discloses a culture medium for constructing a colorectal cancer peritoneal metastasis micro-tumor cell model and application of the culture medium. The invention relates to the technical field of biology, and provides a culture medium for culturing a micro-tumor model for colorectal cancer peritoneal metastasis. The invention discloses a cholera toxin immunopotentiator, which is composed of an antibacterial and antifungal agent three antibody, HEPES, GlutaMax, a human recombinant protein EGF, a human recombinant protein bFGF, a human recombinant protein HGF, a human recombinant protein Noggin, a human recombinant protein R-spondin 1, a human recombinant protein GDNF, SB202190, A83-01, Primocin, N-acetyl-L-cysteine, nicotinamide, an N2 additive, cholera toxin, B27, ITS-X, Y-27632, Galunissertib and a basal culture medium. The culture medium and the culture method disclosed by the invention can effectively improve the culture success rate of colorectal cancer peritoneal metastasis and focus combination.

The invention discloses a method for improving cell growth and expression by changing an iron form in a culture medium, and relates to the technical field of cell culture, the culture medium comprises a basic culture medium, a supplemented culture medium and an iron source; wherein the iron source is FeCl3, the basic culture medium and the supplementary culture medium are commercially available culture media, the iron source is not added or removed, and the culture media further contain a three-dimensional culture material; the redox balance of the culture medium can be maintained, oxidative stress damage of cells is prevented, hydrolysis or precipitation is not prone to occurring, the preparation process of the culture medium is simplified, and the requirements of cell growth and expression are met.

The invention belongs to the field of stem cell culture, and relates to a pluripotent stem cell culture medium, which comprises an E6 basal culture medium, 2 ng / mL of TGF-beta1, 0-50 ng / mL of FGF-2, and 0.01-1 [mu] M of SUN11602. According to the technical scheme, proliferation and pluripotency of the hPSC are effectively maintained in a mode of combining small chemical molecules with low-concentration FGF-2.