168 results about "Basal medium" patented technology

The invention belongs to the technical field of biomedicine, and relates to a method for increasing the yield of pancreas islet organs differentiated by pluripotent stem cells and improving the stability. The method comprises the following steps: (1) inoculating pluripotent stem cells, and culturing by adopting a basic culture medium containing Y-27632; (2) changing the liquid, and continuously culturing by adopting a basic culture medium; (3) culturing for 1 day by using an S1D1 culture medium, and then culturing for 2-3 days by using an S1D2 culture medium; (4) changing the liquid, and culturing for 2-3 days by adopting the S2 culture medium; (5) changing the liquid, and culturing for 1-2 days by adopting an S3 culture medium; (6) changing the liquid, and culturing for 4-7 days by adopting a culture medium S4; (7) changing the liquid, and culturing for 3-10 days by adopting an S5 culture medium; and (8) carrying out cell sphere repolymerization, and culturing for 3-21 days by using an S6 culture medium to obtain the pancreas islet organ. The present invention increases the yield and differentiation efficiency at each differentiation stage and increases the scale yield at the cellular level.

The invention relates to the technical field of cell biology, in particular to a culture medium for inducing neural stem cells and application thereof.The culture medium for inducing the neural stem cells comprises a neural ectoderm induction culture medium and a neural stem cell induction culture medium; the neural ectoderm induction culture medium is prepared from a basic culture medium, an N-2 additive, a B-27 additive, an inhibitor SB431542 and an inhibitor Dorsomorphin, the neural stem cell induction culture medium is prepared from a basic culture medium, an N-2 additive and a B-27 additive, the basic culture medium is composed of a DMEM / F12 culture medium, a Neurobase culture medium, GlutaMax and non-essential amino acid NEAA. The problems that neural stem cells obtained through an existing single-layer adherent induction method based on small molecules or growth factors are uneven in differentiation and low in purity are solved.

The invention belongs to the field of stem cell culture, and relates to a pluripotent stem cell culture medium, which comprises an E6 basal culture medium, 2 ng / mL of TGF-beta1, 0-50 ng / mL of FGF-2, and 0.01-1 [mu] M of SUN11602. According to the technical scheme, proliferation and pluripotency of the hPSC are effectively maintained in a mode of combining small chemical molecules with low-concentration FGF-2.

The invention provides a culture method and an acclimatization and transplantation method for coptis chinensis test-tube plantlets, and belongs to the technical field of plant tissue culture. The method comprises the following steps: (1) disinfecting a coptis chinensis explant, inoculating the disinfected coptis chinensis explant into an MS basal culture medium, and culturing to obtain a sterile seedling; (2) inoculating the aseptic seedlings into an adventitious bud culture medium for adventitious bud culture to obtain adventitious buds; (3) inoculating the adventitious buds into a proliferation culture medium for proliferation culture to obtain proliferation seedlings; and (4) inoculating the proliferation seedlings into a rooting culture medium for rooting culture to obtain the coptis chinensis test-tube plantlets. When the adventitious bud, proliferation and rooting culture medium is used for culturing the coptis chinensis test-tube plantlet, the proliferation coefficient of the test-tube plantlet reaches 3.0, the rooting rate reaches 96.3%, and the survival rate of acclimatization and transplantation reaches 91.5% through seedling acclimatization and transplantation. The culture method provided by the invention is used for preserving the germplasm resources of the coptis chinensis, and provides a basic guarantee for the germplasm improvement of the subsequent medicinal material coptis chinensis.

A method for producing mycelium biomass. The method comprises obtaining a base media composition and an antifoam solution; sterilizing a mixture of the base media composition and the antifoam solution

A method for high-density fermentation production of ULP involves culturing yeast containing a ULP expression vector in a basal medium comprising a carbon source (glycerol) and a nitrogen source (histidine and / or ammonium dihydrogen phosphate). This invention, through high-density yeast fermentation and employing an improved culture medium and process, significantly improves the yield and quality of ULP. The final expression level is 3-5 times higher than that achieved by conventional E. coli fermentation methods, reducing ULP production costs by at least 25%. This reduces the cost of related recombinant protein drugs and allows for wider application of this high-quality system in recombinant protein drug development.

The application belongs to the field of biological medicine, and discloses a culture medium, which comprises a basic culture medium, and the basic culture medium is added with ITS-A: 1x, arginine: 15-20 mu g / ml, SPP1: 3-5 mu g / ml and S100A9: 3-5 mu g / ml. The culture medium is particularly suitable for microtumor culture of MTC model and has good cell viability. Meanwhile, the application also provides application of the culture medium and a microtumor culture method.

This invention discloses a NK cell culture kit, its culture method, and its application. The kit includes an amplification medium, a high-efficiency induction medium, an NK-A coating solution, an NK-B mixture, and an NK-C mixture. The amplification medium contains basal medium, NAD+, human serum albumin, transferrin, β-glucan, glutathione, β-mercaptoethanol, and linoleic acid. The high-efficiency induction medium, in addition to the amplification medium components, contains soybean peptides, nicotinamide, inulin, and N-acetyl-L-cysteine. The NK-A coating solution contains heparin sodium, monoclonal antibodies, and antibodies. The NK-B mixture contains Inbakicept, IL-2, and IL-15. The NK-C mixture contains linolenic acid, IL-2, and IL-18. The Inbakicept factor in the kit can activate NK cells and enhance their cytotoxicity; linolenic acid can inhibit T cell proliferation, increase NK cell purity, and enhance cell efficacy.

The invention relates to the technical field of biology, and discloses a construction method of a gastric cancer organoid culture system, which comprises the following steps: preparation of a conditioned medium: culturing L-WRN cells and collecting the conditioned medium; preparing a gastric cancer organoid culture medium, and mixing the conditioned culture medium, the basic culture medium, a plurality of growth factors and additives; and extraction and culture of organoid: separating cells from gastric cancer tissues, mixing the cells with matrigel, and culturing by using the prepared culture medium. According to the method, the repeatability and the stability of the organoid model are improved, the response consistency of the organoid in drug screening is improved, and the reliability of the model in disease mechanism research and personalized medical application is guaranteed.

The application discloses a kind of preparation and suspension culture of blackland callus method, belong to the field of industrial biotechnology.It includes the following steps: after sterilization, take flower stem, access pH value is 5-7 in induction medium, under the condition of temperature is 20-30 DEG C and no light, induction culture 15-21 days, namely obtain blackland callus, wherein, the induction medium is made of MS basic medium, sucrose 25-35g / L, agar 6-7g / L, 6-BA 1.0-2mg / L, NAA 0.5-1.5mg / L, 2, 4-D 0.3-1.0mg / L and coconut water 0.5-1.5g / L composition.Under aseptic conditions, clamp the growth state of good blackland embryogenic callus 30g, inoculation is carried out in 200mL / 500mL pH value is 6.0 in MS liquid medium, temperature 25 DEG C, 110 rpm full light oscillation culture, subculture period 7 days, when subculture, the mixture of cell and culture fluid is mixed with fresh culture fluid according to 1:1 volume ratio, after equal division, continue to culture, namely obtain blackland suspension cell, wherein, the liquid medium is made of MS basic medium, sucrose 30g / L, 6-BA 1.5mg / L, NAA 0.5mg / L, 2, 4-D 0.5mg / L and coconut water 1.5g / L composition.The callus out of the rate of 88.6% is realized, and the induction rate of embryogenic callus that can be suspended culture is 68.6%-91.8%, and good callus state is provided for large-scale culture blackland cell.

The invention discloses an improved culture medium containing egg yolk protein and a chicken embryo extract and a preparation method of the improved culture medium. The culture medium is mainly composed of egg yolk protein, a chicken embryo extract and a basic culture medium component according to a specific proportion. The improved culture medium is rich in various nutritional ingredients such as proteins, amino acids, vitamins and cell growth factors, can effectively promote the growth and proliferation of various cell lines and improve the efficiency and quality of cell culture, can be widely applied to the field of in-vitro culture of animal cells, provides a choice with higher quality and better cost effectiveness for cell culture, and has a wide application prospect. The problems that a traditional culture medium is insufficient in nutritional ingredients, poor in cell culture effect and the like are solved.

This invention discloses a method for cultivating Spirulina, relating to the field of microalgae biotechnology, aiming to overcome the shortcomings of existing technologies in terms of low Spirulina biomass yield and protein content. The method includes: in a first cultivation stage, inoculating Spirulina into a basal culture medium and conducting photoautotrophic cultivation at a light intensity of 5000-10000 lux until the optical density (OD560) reaches 0.6-0.8; in a second cultivation stage, reducing the light intensity to 1000-3000 lux and adding calcium alginate-encapsulated sodium bicarbonate microspheres, cultivating until the algal cell dry weight reaches 1.5-2.0 g / L; in a third cultivation stage, increasing the light intensity to 8000-12000 lux and adding sodium nitrate slow-release granules supported on anion exchange resin. This invention can achieve a synergistic increase in Spirulina biomass dry weight and protein content.

The invention relates to the technical field of cell culture, and provides an NK cell in-vitro amplification culture method which comprises the following steps: S1, separating to obtain a mononuclear cell of peripheral blood, resuspending the mononuclear cell by using a serum-free NK cell basic culture medium, and then inoculating the mononuclear cell to an NK cell activation culture medium for incubation; s2, culturing to the third day, supplementing the NK cell to activate the culture medium for amplification, then supplementing the NK cell to activate the culture medium once every other day, and culturing to the 19th day to obtain high-purity NK cells; the NK cell activation culture medium is a serum-free NK cell basal culture medium which contains IL-2 (Interleukin-2), IL-12 (Interleukin-12), IL-18 (Interleukin-18), IL-21 (Interleukin-21), a CD16 antibody, a CD56 antibody, 4-1BBL (4-1BBL), nicotinamide, sodium diethyldithiocarbamate, polyinosinic acid and plasma. The method is simple and convenient to operate and short in culture period, the amplification efficiency reaches up to 1050 times within 19 days, and the NK cells are high in purity and accord with clinical treatment standards.

The present disclosure relates to methods of making recombinant proteins (e.g., monoclonal antibodies) in rat hybridoma cells (e.g., YB2 / 0) with high productivity, product quality, and robust functional activity by modifying cell culture process parameters and / or basal media and feed combinations. The present disclosure also relates to commercial-scale production (e.g., 10,000 L-25,000 L) of recombinant proteins (e.g., monoclonal antibodies) in rat hybridoma cells. The present disclosure also relates to recombinant proteins (e.g., monoclonal antibodies) and compositions (e.g., pharmaceutical) that are made using the methods disclosed herein, and which have a unique glycosylation profile.

The invention discloses a chemical defined culture medium formula for subculture of CHO-K1 host cells and high expression of an engineering cell strain product of a stable cell line constructed by taking CHO-K1 cells as hosts. The main components of the feed only comprise amino acids, inorganic salts, vitamins, trace elements and other components limited by chemical components. According to the invention, the growth density and motility of the engineering cell strain taking CHO-K1 as the host are improved, the tolerance of cells is increased, the maintenance period of the cells is prolonged, the product expression quantity of the CHO-K1 engineering cell strain is improved, and the cost of the culture medium is greatly reduced.

The invention relates to an adipose-derived stem cell culture medium containing wormwood extract and a preparation method of the adipose-derived stem cell culture medium, and belongs to the technical field of biology. The adipose-derived stem cell culture medium contains a DMEM (Dulbecco Modified Eagle Medium) basal culture medium, and also contains the following components: 5 to 7 [mu] g / mL of wormwood extract, 1 to 1.5 [mu] g / mL of phosphatidylserine, 0.02 to 0.03 [mu] g / mL of D-calcium pantothenate, 1.2 to 1.5 [mu] g / mL of sialic acid, 0.06 to 0.08 [mu] g / mL of eucommia male flower peptide, 0.3 to 0.35 [mu] g / mL of transferrin and 0.3 to 0.4 mg / mL of bovine serum albumin. The prepared adipose-derived stem cell culture medium has a good promoting effect on proliferation of adipose-derived stem cells from rabbits and mice, the added wormwood polysaccharide extract has a good bacteriostatic effect, and the safety and stability of the culture medium can be effectively maintained.

The invention discloses a novel ginseng stem cell culture system which comprises a basic culture medium and a growth regulation composition, and the basic culture medium comprises ammonium sulfate, monopotassium phosphate, magnesium sulfate heptahydrate and calcium chloride; the growth regulating composition comprises 2, 4-dichlorphenoxyacetic acid, zeatin, salicylic acid and natural polysaccharide. The basic culture medium also comprises casein hydrolysate, coconut water extract and cane sugar. The composition also comprises an anti-oxidation composition. The invention further discloses a disposable flexible bioreactor for ginseng stem cell culture, the bioreactor comprises a tank body made of a flexible material, an air distribution device, a sterile sampling device and an online parameter monitoring device, and the top of the tank body structure is a cylindrical body and is provided with an inoculation opening, a culture medium liquid supplementing opening, an air outlet and a sampling opening; the bottom is an inverted cone provided with a parameter monitoring device interface and an air inlet, and the air inlet is connected with a ventilation device and an air flow adjusting device. The invention also discloses application of the culture system and the bioreactor.

The invention relates to the technical field of biological medicines, in particular to a maintenance solution for culturing influenza viruses as well as a preparation method and application of the maintenance solution. The maintenance liquid comprises a basic culture medium and effective components, the effective components comprise amino acids, vitamins, salts and a virus nutrition enhancer, and the virus nutrition enhancer is a microorganism induced culture extract. The extract is prepared by inoculating fermentation microbial bacteria, performing induced culture through a plant induction factor extract containing American ginseng, polygonum cuspidatum and golden cypress, and then performing autolysis and enzymolysis in the presence of compound metabolic regulatory factors, namely trichosalix A and N-acetylcysteine. The maintenance fluid can significantly enhance host cell metabolism, inhibit cell antiviral response and delay cell apoptosis after infection, so that the culture yield and process stability of influenza viruses are significantly improved under the condition of no dependence on serum.

A method of preparing at least once cell suspension product for treating wastewater, comprising germinating seeds from one or more plants selected from the group consisting of M. peregrine, S. Potatorum, O. ficus-indica, O. basilicum, and H. tuberculatum to provide plant sprouts; cutting the sprouts to provide plant parts; adding the plant parts to a culture medium; adding a basal medium to the culture medium to initiate development of the cell suspension; maintaining the cell suspension for a period of time to harvest callus in the cell suspension; and isolating at least one cell suspension product from the cell suspension.

The invention relates to the technical field of nerve cell differentiation, and discloses a culture medium for promoting neural differentiation of SH-SY5Y cells. The culture medium disclosed by the invention is prepared from the following components: tazarotene (tazarotene) and a basic culture medium. The culture medium can promote SH-SY5Y cells to present neuron-like differentiation within 5 days, significantly improves the length of neurite and the expression level of neuron markers, and is low in cytotoxicity. The invention provides an efficient, safe and standardized new tool for neural disease in-vitro model construction, drug screening and mechanism research.

The invention belongs to the technical field of biological medicine, and particularly relates to a mycoplasma synoviae culture medium capable of improving strain titer and a preparation method of the mycoplasma synoviae culture medium. The mycoplasma synoviae culture medium comprises a basic culture medium and auxiliary components, the basic culture medium is prepared from HEPES, milk protein hydrolysate, MEM, beef heart extract powder, coenzyme I, 1% phenol red, magnesium sulfate heptahydrate, L-cysteine hydrochloride, lactose-N-tetrasaccharide, disodium clodronate and water; the auxiliary components comprise porcine serum and penicillin. According to the mycoplasma synoviae culture medium obtained by the preparation method, the passage time of the mycoplasma synoviae is shortened, and the strain titer is improved. The inactivated vaccine prepared from the chicken mycoplasma synoviae cultured by the culture medium is good in safety and immunogenicity, can effectively prevent infection of the chicken mycoplasma synoviae, and has a good application prospect.

The present application provides a method for developing a basal culture medium, a method for developing a basal culture medium formulation and system thereof. The method for developing a basal culture medium comprises, (1) determining a regression model for selected culture indicators to predict the culture indicators of a basal culture medium; (2) acquiring an addition range of each component in the basal culture medium, and enumerating and randomly selecting to generate a plurality of candidate basal culture medium formulations; (3) predicting the culture indicators by adopting the regression model and recommending a basal culture medium formulation; and (4) performing cell culture experiments to verify the culture indicators of the recommended basal culture medium formulation.

The invention provides an induced pluripotent stem cell culture medium and a culture method of induced pluripotent stem cells. The induced pluripotent stem cell culture medium comprises a basic culture medium, a composite additive A and a composite additive B, the composite additive A comprises 5-30 [mu] g / mL of recombinant human insulin growth factors, 1-5 ng / mL of recombinant human transforming growth factors, 50-200 ng / mL of recombinant human basic fibroblast growth factors, 7-20 [mu] g / mL of sodium selenite, 0.5-2 mg / mL of albumin, 50-70 [mu] g / mL of ascorbic acid and 5-20 [mu] g / mL of transferrin; the composite additive B is prepared from 50 to 300mg / mL of a Stock B solution, 0.5 to 5 percent (v / v) of a non-essential amino acid solution, 50 to 200ng / mL of DL-piperidinecarboxylic acid, 50 to 200mu g / mL of gamma-aminobutyric acid, 0.1 to 1 percent (v / v) of a lipid concentrated solution, 0.5 to 5 percent (v / v) of an L-alanyl-L-glutamine solution and 0.1 to 0.1 percent of mercaptoethanol. The culture medium for the induced pluripotent stem cells provided by the invention can ensure the self-renewal capability of the iPSCs in a long-term passage process; and the genetic stability of the iPSCs cells is optimized according to the genome integrity and pluripotent state.

The application discloses a kind of construction method and application of seawater fish muscle stem cell line, culture medium with basic medium as background medium;Culture medium includes LIF growth factor, EGF growth factor and leopard bristletooth-derived FGF2 protein;The final concentration of leopard bristletooth-derived FGF2 protein is 20~30 ng / mL, 30~45 ng / mL, 45~55 ng / mL or 55~70 ng / mL. Through the medium, immortalized seawater fish muscle stem cell line with good stability, cell division vigorous, short subculture time can be constructed, and can be stably subcultured for more than 50 generations. It provides raw materials for exploring the function of myoblasts, gene regulation mechanism, and provides the prerequisite for in vitro verification experiment for further exploring the influence of seawater fish muscle stem cells on growth rate, and lays a foundation for good strain breeding of seawater fish.

This invention relates to the field of biomedical technology, specifically to a serum-free and protein-free culture medium and its preparation method for enhancing NK cell expansion and cytotoxic activity. The serum-free and protein-free culture medium comprises a basal medium, an AMPK-HIF1α regulator, immunomodulatory factors, mitochondrial function enhancers, antioxidants, trace elements, and a pH buffer. The AMPK-HIF1α regulator is composed of α-ketoglutarate at a final concentration of 1.4-1.6 mmol / L and AICAR at 0.4-0.6 mmol / L. This culture medium activates the AMPK-HIF1α pathway through precise formulation of α-ketoglutarate and AICAR, and optimizes the metabolic-antioxidant network in synergy with nicotinamide ribose and glutathione, thus solving the industry problem of the incompatibility between expansion and function in serum-free NK cell culture.

This invention discloses a culture medium, preparation method, and culture method for the industrial-scale preparation of mesenchymal stem cells, belonging to the field of cell therapy technology. The culture medium comprises a basal medium, cell growth factors, cell proliferation promoters, a vitamin mixture, a nucleoside mixture, and an amino acid mixture; wherein the cell growth factors are selected from one or more of TGF-β, bFGF, VEGF, PDGF, EGF, and IGF. Through the synergistic effect of the composite nutrient system, this invention significantly improves the proliferation rate and cell viability of mesenchymal stem cells, maintains stem cell stemness, trilineage differentiation ability, and secretion levels of key cytokines (VEGF, HGF, TGF-β, IL-10, etc.); it can be stably and continuously passaged up to the 15th generation without abnormal changes in genetic material. This culture medium contains no animal-derived components, has high batch-to-batch stability, and is cost-controllable, making it suitable for GMP-level large-scale industrial preparation of mesenchymal stem cells from various sources such as umbilical cord, bone marrow, adipose tissue, and placenta.

This invention relates to the field of gastric cancer organoid microarray technology, and more particularly to a gastric cancer organoid microarray culture medium and its applications. The invention provides a novel culture medium adapted for gastric cancer organoid microarray culture. The medium components include basal medium, penicillin / streptomycin dual antibiotics, antibacterial agents, hydroxyethylpiperazine ethanesulfonic acid, B-27 additive, Glutamax culture medium additive, R-vertebral protein 1, scutellarin B, and tetramethylpyrazine, among other components. This invention solves key problems in existing technologies such as slow growth, morphological instability, poor heterogeneity adaptation, and low long-term culture activity of gastric cancer organoids, providing an ideal experimental model for high-throughput screening and precision medicine applications on microarrays.

The invention discloses a stem cell-based microvesicle as well as a preparation method and application thereof, belongs to the technical field of biological tissue engineering, and particularly relates to a preparation method of the stem cell-based microvesicle, which comprises the following steps: culturing stem cells until the cell fusion degree is 80-90%, performing hypoxia treatment, and cleaning for 2-3 times; standing and culturing the cleaned stem cells, then adding a calcium ion carrier, and carrying out ultrasonic treatment; the serum-free basic culture medium containing the calcium ion carrier is immediately absorbed and removed, the cells are cleaned through PBS, culture is conducted for 24 h, and cell supernatant is collected and merged respectively; performing first centrifugation to remove cell debris; performing second centrifugation to remove dead cells and large cell debris; carrying out third centrifugation to remove organelles; fourth centrifugation is carried out, large vesicles are removed, and a final supernatant is obtained; and filtering and centrifuging to obtain a microvesicle precipitate. The preparation method of the microvesicles aims at systematically improving the yield and biological activity of the stem cell microvesicles through low-oxygen pre-stimulation, ultrasonic-calcium ion carrier synergistic induction and differential centrifugation.

The invention discloses a method for synthesizing acetic acid from carbon dioxide by using sludge microbial flora, which comprises the following steps: step 1, inoculation: taking sludge containing acetogenic bacteria as an inoculum, and adding the inoculum into a reactor containing a basic culture medium; step 2, establishing an initial environment: introducing mixed gas for providing a carbon source and an energy source into the reactor, aerating, and replacing air in the reactor; step 3, implementing a nutrition stress stage: after aeration in the step 2 is finished, sealing the reactor, and carrying out constant-temperature culture for 10-15 days; and 4, strengthening culture in a recovery period: after the step 3 is finished, connecting a mixed gas providing a carbon source and an energy source with the reactor, executing periodic constant-temperature culture, and regularly replacing the basic culture medium in the periodic constant-temperature culture process. The method is simple to operate, low in cost and high in acetic acid yield.

This invention discloses a strain of Rhodotorula glutinis I7Y2 that promotes cell proliferation in a serum-free environment and its applications. Rhodotorula glutinis ( Rhodotorula mucilaginosa The yeast strain I7Y2 was deposited at the Guangdong Provincial Microbial Culture Collection Center (GDMCC) on March 13, 2026, with accession number GDMCC No: 67951. This invention has a good effect on promoting Vero cell proliferation, specifically manifested in: (1) the yeast involved in this invention can produce various active substances such as amino acids, polypeptides, sanshool, and inositol to meet the nutritional needs of cell growth; (2) it can effectively improve the viability and density of Vero cells in the basal culture medium; (3) it can effectively improve the proliferation rate and migration rate of Vero cells in the basal culture medium; and (4) it can effectively reduce the apoptosis rate of Vero cells and the expression of apoptosis-related genes in the basal culture medium. Therefore, Rhodotorula glutinis I7Y2 has great application potential in promoting cell proliferation, especially in serum-free culture products.