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Preparation of medicinal protolysate

A technology for hydrolyzing protein and protein, applied in the field of enzymatic protein hydrolysis, can solve the problems of easy loss of autolytic hydrolytic enzyme activity, easy inactivation of autolytic hydrolytic enzyme, long reaction time, etc., to improve physical decline, enhance hematopoietic function, The effect of convenience

Active Publication Date: 2008-09-17
康哲(湖南)制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many sources of enzymes in enzymatic hydrolysis. Various hydrolytic enzymes in biological tissues can be used to decompose cell tissues, and exogenous enzymes suitable for the reaction system can also be added to decompose cell tissues. There are many types of hydrolytic enzymes with different activities, and they will also be affected by other characteristics in the body. The activity of autolytic hydrolytic enzymes in the body is easy to lose
Moreover, using the autolyzed hydrolase present in the body to hydrolyze protein has the disadvantages of long reaction time, low efficiency, low yield, and easy inactivation of the autolyzed hydrolase.

Method used

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  • Preparation of medicinal protolysate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Wash the beef, drain it, cut into small pieces, mince 60kg, take 17kg of shelled eggs, mix with 23kg of soybean meal, add 100kg of water, stir evenly, boil for 30 minutes, cool in a water bath to 30°C, add 1.5kg of protease, Hydrolyze at a temperature of 30°C and adjust the pH to 5 with ammonia water to obtain a hydrolyzate; filter the hydrolyzate in a separator, discard the filter residue, boil the filtrate, refrigerate at -10°C for 3 hours, and filter the supernatant. Concentrate and filter to obtain a concentrated solution. Add 18kg of sucrose, 450g of ethylparaben, 150g of menthol and purified water to the concentrated solution to make 150L, ​​stir evenly, filter, and divide into 30m (glass bottles are washed with drinking water, then washed with purified water, and placed in a hot air circulation oven 100 DEG C for 1 hour drying), a total of 4950 bottles, sterilized for 30 minutes, packaged to obtain oral liquid products.

Embodiment 2

[0022] Wash the crocodile meat, drain, cut into small pieces, mince 20kg, take 40kg of eggs after shelling, mix with 40kg of soybean meal, add an appropriate amount of 250kg of water, stir well, boil for 30 minutes, cool in a water bath, to 45°C, add protease 0.1 kg, at a temperature of 45°C, hydrolyzed with ammonia water to pH 8 to obtain a hydrolyzate; filter the hydrolyzate, discard the filter residue, boil the filtrate, refrigerate at -5°C for 3 hours, and filter the supernatant. Concentrate and filter to obtain a concentrated solution. Add concentrated solution, 18kg sucrose, 450g ethylparaben, 150g menthol and purified water to make 150L, ​​stir evenly, filter, pack in 30m (wash the glass bottle with drinking water, then wash with purified water, put hot air circulation Oven drying at 100°C for 1 hour), a total of 4935 bottles, sterilized for 30 minutes, and packaged.

Embodiment 3

[0024] Wash the beef, drain it, cut into small pieces, mince 70kg, take 10kg of shelled eggs, 20kg of soybeans, mix them, add 500kg of water, stir evenly, boil for 30 minutes, cool in a water bath, and add 1kg of protease. Hydrolyze at a temperature of 30°C and pH 5 to obtain a hydrolyzate; filter the hydrolyzate with a separator, discard the filter residue, boil the filtrate, refrigerate at -10°C for 3 hours, and filter out the supernatant. Concentrate the above-mentioned filtrate under reduced pressure at 80°C until the specific gravity is 1.05-1.15, stop the concentration, and filter to obtain the hydrolyzed protein concentrate. Spray-drying: Spray-dry under the conditions of air inlet temperature 165-170°C and outlet air temperature 100-105°C to obtain white protein powder. It can be used for sub-packaging with specifications of 5g / bag, 10g / bag, and 15g / bag (packaging) to obtain finished powders.

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Abstract

The invention discloses a preparation method of medicinal hydrolysis protein. Mixing the animal protein 20 to 80 weight account, egg protein 10 to 50 weight account and vegetable protein 10 to 50 weight account, adding water 0.5 to 5 times amount, stirring uniformly, boiling, refrigerating, leaching the supernatant, concentration, filtration, so that the hydrolysis protein concentration liquid is acquired. Spray drying the hydrolysis protein concentration liquid to acquire the hydrolysis protein powder. The total nitrogen rate of the hydrolysis protein manufactured by the method of the invention is more than 12 percent, and the rate of return of the amino acid is up to more than 8 percent. The invention is used for the clinical medicine or the addition agent of nutritional food, has high nutrition, easy absorption and popularization. The invention is clinically used for hypoproteinemia, and dystrophia and malnutrition caused by various diseases, also used for burn, fracture and malunion of wound after operation. The invention has the advantages of obvious therapeutic effect, good taste, convenient take, big market requirement and significant economic benefit.

Description

technical field [0001] The invention relates to a method for enzymatically hydrolyzing protein, in particular to a method for preparing hydrolyzed protein. Background technique [0002] At present, the protein hydrolysis technology at home and abroad mainly adopts three kinds of process methods: acid hydrolysis method, alkali hydrolysis method and enzyme hydrolysis method. Among them, acid hydrolysis and alkali hydrolysis belong to chemical hydrolysis, while enzymatic hydrolysis belongs to biological enzyme hydrolysis. The acid hydrolysis method is usually hydrolyzed with a strong acid, namely sulfuric acid or hydrochloric acid. The molar concentration of the acid is 6 mol / liter, and the hydrolysis is carried out for more than 20 hours at a high temperature of 110°C. Afterwards, asparagine and glutamic acid were completely hydrolyzed into aspartic acid and glutamic acid respectively, and tryptophan was completely destroyed; serine and threonine were partially hydrolyzed, wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/02A23L1/305A61P3/02A61P37/04A61P7/06A61P7/00A61K38/01A23L33/17A23L33/185
Inventor 钟恒费善芬
Owner 康哲(湖南)制药有限公司
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