58results about How to "Significant effect" patented technology

The invention belongs to the technical field of traditional Chinese medicine, and particularly relates to a medicine patch needle for preventing and treating allergic rhinitis and a preparation method and application thereof. The medicine patch needle mainly comprises a traditional Chinese medicine composition and a medicine patch thumbtack needle, and the traditional Chinese medicine composition is prepared from, by weight, 30-60 parts of radix astragali, 15-30 parts of radix angelicae sinensis, 10-25 parts of radix saposhnikoviae, 10-30 parts of rhizoma atractylodis, 10-30 parts of rhizoma acori graminei, 5-15 parts of herba asari, 10-30 parts of rhizoma chuanxiong, 10-20 parts of radix angelicae and 1-5 parts of borneol. The medicine needle patch can be used for preventing and treating allergic rhinitis, mild and lasting acupuncture stimulation is achieved on the skin part of the acupoint, the medicine can rapidly enter the acupoint along with the needle hole, in other words, the acupoint achieves the effects of harmonizing qi and blood, relieving stuffy nose and removing pathogenic qi under the dual action of the acupuncture stimulation and the medicine, and the medicine needle patch has the remarkable preventing and treating effect on the allergic rhinitis.

The invention discloses a freckle-removing and whitening mask which is prepared from the following raw materials: chrysanthemum, pearl, radix angelicae, almond, poria cocos, rhizoma atractylodis macrocephalae, peach kernel, ginseng, mung bean, bletilla striata, cortex moutan, rhodiola rosea, silkworm larva, Chinese yam, honeysuckle and the like. The facial mask is a pure traditional Chinese medicine facial mask, has remarkable curative effects on whitening, removing freckles, removing acnes and minimizing pores, and can effectively reduce facial skin damage, effectively inhibit growth of facial germs, promote vigor of subcutaneous capillaries of the damaged face, improve metabolism of the skin and enable the facial skin to reappear in a compact and refined state. In addition, the facial mask can enhance the ability of facial skin to resist invasion and stimulation of external germs, form a natural protective barrier for the facial skin, well repair, moisten and care the facial skin, and keep the facial skin comfortable, beautiful and healthy for a long time. Pure traditional Chinese medicine raw materials are selected, chemical components are not added, and harm to facial skin and the body is avoided.

The present application belongs to the technical field of traditional Chinese medicine composition, and relates to a traditional Chinese medicine transdermal external preparation for treating vulvar hypopigmentation disease. The preparation is a paste prepared by mixing traditional Chinese medicine powder and horse oil. The traditional Chinese medicine powder is composed of the following raw materials by weight: Xuchangqing 10-12, Pugongying 10-15, Difusiduo 12-15, Tuzifuding 12-15, Baiji 10-15, Huzhang 9-12, Quansie 6-10, Diyu 10-12, Xuezhi 10-12, Danggui 6-10, Rushang 6-10, Miedu 6-10, Bingpian 1-3. The traditional Chinese medicine transdermal external preparation has definite and significant effects on vulvar hypopigmentation disease, especially in relieving intractable pruritus, promoting pigment recovery and improving skin sclerosis / atrophy. The traditional Chinese medicine transdermal external preparation has high safety, few adverse reactions and low recurrence rate.

The invention discloses a traditional Chinese medicine monomer composition derived from chrysanthemum plants and used for treating diabetic retinopathy as well as a preparation method and application of the traditional Chinese medicine monomer composition. The traditional Chinese medicine monomer composition prepared from the chrysanthemum plant as the raw material is high in safety, small in side effect and definite in component and is prepared according to a specific ratio, and pharmacodynamic experiments prove that compared with an ethanol extract, the traditional Chinese medicine monomer composition is more remarkable in curative effect on diabetic retinopathy, and the curative effect of the traditional Chinese medicine monomer composition on diabetic retinopathy is better than that of a traditional Chinese medicine composition on diabetic retinopathy. The composition can improve the pathological state of diabetic retinopathy from multiple targets and multiple ways, specifically can inhibit body mass increase caused by high glucose, reduce blood glucose level, improve retinal tissue structure damage, recover retinal tissue thickness, inhibit retinal cell apoptosis and inflammatory response, reduce the expression level of HIF-1alpha and VEGF protein in retinal tissue, and can be used for treating diabetic retinopathy. Scientific basis and technical support are provided for research and development of novel drugs for treating the diabetic retinopathy, and important clinical significance and market application prospects are achieved.

This invention relates to the field of traditional Chinese medicine composition technology, and particularly to a method of using, preparing, and applying a traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from raw materials comprising a first component, a second component, a third component, and a fourth component: the first component comprises a mixture of raw oyster shell, prunella vulgaris, and fritillaria thunbergii; the second component comprises a mixture of angelica sinensis, scorpion, notoginseng, and cattail pollen; the third component comprises one or more of lindera strychnifolia, chuanxiong rhizome, cyperus rotundus, and corydalis yanhusuo; the fourth component comprises one or more of fenugreek, angelica dahurica, and fennel; the mass ratio of the first component to the second component is 1.3–2:1. This traditional Chinese medicine composition can not only significantly prevent and alleviate dysmenorrhea symptoms in patients with acute menstrual cramps (AM), but also improve other accompanying symptoms during menstruation in AM patients, such as heavy menstrual bleeding.

This invention belongs to the field of traditional Chinese medicine and relates to a traditional Chinese medicine composition for treating dry eye syndrome, a fumigation and washing agent, its preparation method and application. The traditional Chinese medicine composition for treating dry eye syndrome, by weight, includes 20-30 parts of chrysanthemum extract powder, 15-20 parts of wolfberry extract powder, 12-18 parts of ophiopogon japonicus extract powder, 5-10 parts of polygonatum odoratum extract powder, 10-20 parts of angelica sinensis extract powder, 5-15 parts of astragalus membranaceus extract powder, 8-14 parts of salvia miltiorrhiza extract powder, 11-17 parts of ampelopsis japonica extract powder, and 10-15 parts of sedum sarmentosum extract powder. This invention provides a traditional Chinese medicine composition comprising dried powders of chrysanthemum extract, dried powder of wolfberry extract, dried powder of ophiopogon japonicus extract, dried powder of polygonatum odoratum extract, dried powder of angelica sinensis extract, dried powder of astragalus membranaceus extract, dried powder of salvia miltiorrhiza extract, dried powder of ampelopsis japonica extract, and dried powder of sedum sarmentosum extract. The total effective rate of this traditional Chinese medicine composition in treating dry eye syndrome can reach over 90%.

The application discloses application of tamibarotene in preparation of a medicine for preventing and / or treating liver fibrosis, and belongs to the technical field of biological medicine. The application finds that tamibarotene has a significant anti-liver fibrosis effect for the first time. Experimental results show that in two kinds of liver fibrosis animal models induced by CCl4 and TAA, tamibarotene can significantly down-regulate the expression of alpha-SMA and type I collagen, reduce the liver inflammatory response, and improve liver function. In particular, the anti-fibrosis effect of tamibarotene is significantly better than that of RARbeta selective agonist KCL-286 and RARalpha selective agonist Am580, and an unexpected technical effect is achieved. The application provides a new medicine selection for the treatment of liver fibrosis, and has a good clinical application prospect and development value.

The invention provides a traditional Chinese medicine external application composition for treating gastroparesis after brain injury and a preparation method thereof. The traditional Chinese medicine external application composition is prepared from the following components: rhizoma acori graminei, paederia scandens, radix polygoni multiflori preparata, aloe, gastrodia elata, semen aesculi, phyllanthus emblica, stephania tetrandra, turmeric, radix fici simplicissimae, fructus piperis longi, swertia pseudochinensis, rhizoma nardostachyos, lily, ginkgo leaves and cynanchum paniculatum. Dual mechanisms of neuroprotection and gastrointestinal motility regulation are creatively fused, central nervous injury can be improved through components such as rhizoma acori graminei and radix polygoni multiflori preparata, gastrointestinal hormone secretion and slow-wave pace-making functions can be synchronously regulated through components such as paederia scandens and turmeric, and compared with a conventional single-target treatment means, the traditional Chinese medicine composition is more suitable for a complex pathological mechanism of gastric paralysis after brain injury. Meanwhile, the preparation process is simple, the cost is low, external application avoids aggravation of gastrointestinal burdens, common western medicine side effects such as arrhythmia are avoided, clinical operation is convenient, patient tolerance is good, the gastric emptying recovery time is remarkably shortened, and the risk of complications such as aspiration pneumonia is reduced.

The application discloses a medicine composition for treating infant night cough, which is composed of Radix Bupleuri, Radix Paeoniae Alba, Fructus Aurantii Immaturus, roasted Germinated Millet, roasted Millet, Pericarpium Citri Reticulatae, Folium Pyrrosiae, Radix Trichosanthis, Radix Pulsatillae, Radix Anemarrhenae, Radix Peucedani, Radix Trichosanthis, Radix Ophiopogonis, Radix Sophorae Immaturus, and Radix Glycyrrhizae. The medicine composition can be added with pharmaceutically acceptable adjuvants to form preparations. The medicine composition and the preparations can be used for preparing medicines for treating infant night cough.

The application discloses application of delphinidin chloride glucoside or a pharmaceutically acceptable salt thereof in preparation of a medicine for preventing, alleviating or treating cardiorenal metabolic syndrome; the application adopts eNos ‑ / ‑ The drug efficacy is verified by using a db / db animal model, and 10 mg / kg of a low administration dose can significantly improve kidney excretion and filtration function under the condition of severe endothelial injury and metabolic disorder, and effectively reduce a plurality of core biochemical indexes such as urine albumin excretion rate (UACR), serum creatinine (SCr) and urea nitrogen (BUN). The application breaks through the deficiency that existing research is limited to cell experiments or light animal models, and confirms that the substance can effectively reverse myocardial cell hypertrophy, myocardial fibrosis, glomerular sclerosis and other organic pathological injuries from the organ level through cardiac ultrasound detection and histopathological section analysis. The application can provide a new single-drug treatment candidate for clinical treatment of late-stage severe diabetic complications, and is especially suitable for patients with double damage of heart and kidney function.

The present application relates to a kind of salvianolic acid C freeze-dried preparation and preparation method and application, the salvianolic acid C freeze-dried preparation includes salvianolic acid C, pH adjusting agent, stabilizer and excipient.The salvianolic acid C freeze-dried preparation provided in the present application is traditional Chinese medicine extract, and each additive is suitable for administration route, component is safe, and good stability can be achieved, suitable for long-term storage, process is simple, reproducibility is good, easy to large-scale industrial production.The salvianolic acid C freeze-dried preparation of the present application can be injected to administration, also can be atomized to give human respiratory tract, provide a kind of new treatment means for anti respiratory tract influenza virus such as influenza virus A H1N1, influenza virus A H3N2, influenza virus B, parainfluenza virus, respiratory syncytial virus, adenovirus, etc., and have good curative effect.

The invention discloses a traditional Chinese medicine composition for treating diabetic skin itch and a preparation method thereof, and belongs to the technical field of traditional Chinese medicines. The composition is prepared from angelica sinensis, astragalus membranaceus, radix paeoniae rubra, cortex dictamni, rehmannia, ligusticum wallichii, scalded peach kernels, safflower carthamus, fried tribulus terrestris, divaricate saposhnikovia roots and cicada slough according to a specific weight ratio. On the basis of a classic famous formula of the Chinese angelica potion, the Chinese angelica potion is innovatively prepared aiming at the core pathogenesis of qi deficiency, blood stasis, damp-heat and wind-dryness of diabetic skin itch. The radix paeoniae rubra and the radix paeoniae alba are used for enhancing the effects of cooling blood and dissipating blood stasis; peach kernel and safflower are added to strengthen blood circulation activation and collateral dredging; cortex dictamni is added to enhance dampness eliminating and detoxification; cicada slough and herba schizonepetae are used for enhancing wind dispelling and heat clearing, and the nature is Polygonum multiflorum and liquorice are removed, so that the safety is improved. The whole formula has the effects of tonifying qi, promoting blood circulation, eliminating dampness, detoxifying, dispelling wind and moistening dryness. The invention also provides a preparation method of the composition. The composition can be used for preparing medicines for treating skin diseases such as diabetic skin itch and eczema, and is remarkable in curative effect and high in safety.

The invention provides a rhinitis treatment method based on viscera meridian regulation, relates to the technical field of rhinitis treatment, and is characterized by comprising a moxibustion method, a fumigation method, a massage method and an application method. According to the moxibustion method, the Shenque acupoint is subjected to moxibustion by adopting hanging pot moxibustion or stale moxibustion, and the characteristic that the Ren meridian acupoint is communicated with the internal organs is utilized. The traditional Chinese medicine composition has the advantages that overall conditioning is achieved from the function of internal organs, local symptoms and the whole body state are considered, and the limitation of a single therapy is broken through; pure traditional Chinese medicines are combined with physical therapy, safety is high, and side effects of chemical medicines are avoided; clinical observation shows that the curative effect is remarkable, nasal obstruction, running nose, sneezing and other symptoms of a patient are obviously relieved, and the living quality is effectively improved; the operation process is clear and standard, and is easy to popularize and apply in community hospitals, traditional Chinese medicine clinics and family health care; the concept of'Huangdi internal acupuncture 'is combined with the modern traditional Chinese medicine external treatment method for the first time, and a systematized rhinitis conditioning system is constructed.

The application discloses an engineered exosome targeting human urokinase-type plasminogen activator receptor uPAR and a preparation method and application thereof. Firstly, a high-affinity anti-human uPAR single-domain antibody VHH is prepared; then, a human umbilical cord mesenchymal stem cell engineering cell line expressing a VHH-PTGFRN fusion protein is constructed, and the engineered exosome is prepared and purified by combining three-dimensional hypoxic culture, chemical induction and size-exclusion chromatography technology. The engineered exosome comprises four comparative samples, wherein the core sample Exo^(VHH / miR) can simultaneously display the anti-uPAR VHH on the membrane surface to realize targeted delivery and enrich miR-193b-3p in the chamber to realize gene silencing, and the synergistic effect of the two can efficiently remove the uPAR high-expression senescent cells and inhibit the senescence-associated secretory phenotype SASP. The engineered exosome has the advantages of standardized preparation process, high yield and high purity, strong targeting, excellent curative effect and good safety, and can be used for preparing a treatment preparation for anti-aging and aging-related diseases, and has important clinical application value.

This invention discloses an antimicrobial peptide, its pharmaceutical composition, and its applications. The invention optimizes the structure of the antimicrobial peptide through deuteration modification technology, significantly enhancing its targeted binding ability to β-1,3-D-glucan synthase. The affinity of this antimicrobial peptide for the aforementioned target is 9-27 times that of the control peptide. In vitro assays show that its minimum inhibitory concentration (MIC) against nine standard Candida strains is consistently 0.002 μg / mL, exhibiting significantly superior activity compared to fluconazole, anidoxurine, and the control peptide. It also exhibits extremely low cytotoxicity against L02 human normal hepatocytes. In in vivo experiments, in a neutropenic mouse model of Candida albicans infection, the antimicrobial peptide at all doses showed superior reduction in renal fungal load compared to anidoxurine and the control peptide. The antimicrobial peptide of this invention combines highly efficient anti-Candida activity, low cytotoxicity, and excellent in vivo efficacy, providing a safe and effective candidate drug for the treatment of drug-resistant candidiasis and possessing good clinical translational value.

The application belongs to the technical field of biomaterial preparation, and particularly relates to a composite drug delivery system based on macrophage membranes and a preparation method and application thereof. The composite drug delivery system takes a lipid nanoparticle as a drug targeting delivery carrier, coats quercetin through an active phagocytosis mode, coats a macrophage membrane on the surface of the lipid nanoparticle, and constructs a quercetin lipid nanoparticle coated with a macrophage membrane (MCM@QU@LNP). The composite drug delivery system provided by the application can promote drug enrichment in RA lesions, improve local drug exposure, realize precise drug delivery in the RA part, has good biological safety, and reduces system toxicity.

The invention provides a bispecific antibody capable of binding to PD-L1 and VEGF (vascular endothelial growth factor). The bispecific antibody comprises a first binding domain capable of binding to PD-L1 and a second binding domain capable of binding to VEGF. The bispecific antibody provided by the invention is combined with PD-L1, so that the immunosuppression of tumor cells on T cells can be relieved, and the anti-tumor function of an immune system can be recovered; meanwhile, by combining the bispecific antibody with VEGF, the growth of the tumor can be slowed down, the blood supply of the tumor can be reduced, and even the tumor blood vessel can be normalized, so that the immune environment is improved, and the attack ability of immune cells on the tumor is enhanced.

This invention relates to the field of biomedical nanomaterials and pharmaceutical formulations, and discloses a biomimetic nanoenzyme drug for treating gouty arthritis, its preparation method, and its application. The biomimetic nanoenzyme drug is a core-shell structured nanoparticle, comprising, from the innermost layer to the outermost layer: ① a catalytic and antioxidant core, formed by a composite of carbon dots and manganese dioxide; ② a molecularly imprinted polymer layer coating the core, the molecularly imprinted polymer being formed using uric acid as a template molecule; and ③ a platelet cell membrane layer coating the molecularly imprinted polymer layer. This invention utilizes the aforementioned biomimetic nanoenzyme drug for treating gouty arthritis, its preparation method, and its application. This drug, through a multi-level biomimetic assembly strategy, integrates carbon dots (CDs) to enhance antioxidant activity, molecularly imprinted polymers (MIPs) to impart uric acid selectivity, and platelet membrane (PLM) camouflage to improve targeting and compatibility, thereby achieving a safe and highly effective synergistic treatment for gouty arthritis.

This invention belongs to the field of traditional Chinese medicine external preparation technology, specifically providing a traditional Chinese medicine ointment for treating chronic osteomyelitis, bone tuberculosis, and persistent sinus tracts, as well as its preparation method. The traditional Chinese medicine ointment provided by this invention comprises 3-10 parts of *Achyranthes bidentata*, 5-15 parts of dried ginger, 0.3-1 part of red mercury sulfate, 20-50 parts of calcined gypsum, 4-12 parts of gecko, 5-15 parts of buffalo hide, and 40-100 parts of medicinal ointment base. This invention selects *Achyranthes bidentata*, red mercury sulfate, calcined gypsum, gecko, dried ginger, and buffalo hide as the main ingredients, achieving a combination of warming and cooling properties, and a tonifying and purging effect. It can effectively clear deep pus, necrotic tissue, and dead bone, while also warming and unblocking meridians, improving local blood circulation, promoting wound healing, and reducing the irritation of the medication.

The invention relates to a composition with a function of improving bone mineral density, application of the composition and a preparation method of related health-care products or foods, and the composition comprises the following components in parts by weight: 0.5-4.5 parts of D-glucosamine hydrochloride, 0.5-3 parts of calcium carbonate, 0.1-1.5 parts of sodium chondroitin sulfate, 0.5-2.5 parts of American ginseng extract, 0.2-2 parts of salvia miltiorrhiza extract, 0.15-1.5 parts of radix paeoniae alba extract and 0.1-1 part of turmeric extract. The composition is prepared into a medicine, a health care product or a food to increase the bone mineral density. According to the composition, green, natural and high-quality raw materials and auxiliary materials are selected, the composition is prepared from the perspective of traditional Chinese medicine, modern nutriology and modern pharmacology, calcium supplement, articular cartilage protection and inflammation relieving are integrated, the formula plays a comprehensive role in increasing the bone mineral density in different links, and the effect of increasing the bone mineral density under the effect of three pipes is achieved. The composition can be prepared into medicines, health-care products or foods, the preparation process is simple, and the prepared product is green, safe and remarkable in curative effect and has a good market prospect.

The invention discloses an asiatic acid saponin compound and medical application thereof. The asiatic acid saponin compound comprises a compound with a structural formula as shown in a formula (I), and pharmaceutically acceptable salt or ester or solvate of the compound. The compound shown in the formula (I) has anti-inflammatory and anti-ulcer activity, so that the compound can be used for preparing medicines for preventing or treating inflammatory diseases or ulcerative diseases.

This invention discloses an extracellular vesicle derived from Sophora flavescens, its preparation method, and its application. It belongs to the field of biomedical technology. The extracellular vesicles derived from Sophora flavescens are obtained by isolating extracellular vesicles (SF-EVs) from the roots of Sophora flavescens. These vesicles have a particle size of 100-200 nm and contain proteins, lipids, high abundance of miR6300, and various alkaloids. This invention also provides a method for preparing the extracellular vesicles and further provides their application in the preparation of drugs for treating metabolic-associated steatohepatitis (MASH). In vitro and in vivo experiments have demonstrated that SF-EVs can significantly improve lipid accumulation, inflammatory response, and oxidative stress in MASH, with an overall therapeutic effect superior to an equivalent dose of total alkaloids from Sophora flavescens. Through protein / RNA knockout, transcriptome sequencing, and mechanism verification, miR6300 was found to be a key effector of SF-EVs in its anti-MASH effect, and it exerts a synergistic therapeutic effect by dually targeting CYP2B6 (improving lipid metabolism) and CXCL10 (inhibiting inflammation).

The application discloses application of metformin in preparation of a medicine for treating interstitial cystitis, and finds that metformin can reduce pain sensitivity of an interstitial cystitis (IC) rat, reduce edema degree of a bladder of the IC rat, restore bladder capacity of the IC rat, repair the bladder mucosa of the IC rat and reduce submucosal hemorrhage, reduce mast cell infiltration, and has a significant curative effect on interstitial cystitis, and can provide a new medicine for treating interstitial cystitis.

The invention discloses application of piperlongumine in treating and preventing lung injury, and belongs to the technical field of medicines. Aiming at the technical bottlenecks of limited treatment means and high case fatality rate of the existing acute lung injury (ALI) / acute respiratory distress syndrome (ARDS), the invention discovers that piperlongumine can specifically inhibit cGAS-STING signal channel activation for the first time, and expression release of inflammatory factors IFN-beta, IL-6 and TNF-alpha is reduced by regulating phosphorylation of a downstream interferon regulatory factor 3 (IRF3). In-vitro experiments prove that piperlongumine inhibits ISD-induced inflammatory response in a dose-dependent manner, and in-vivo experiments show that piperlongumine can significantly relieve LPS-induced mouse acute lung tissue inflammatory cell infiltration, reduce serum IL-6 and TNF-alpha levels, and improve lung tissue pathological injury. According to the invention, the medicinal application of piperlongumine is expanded, a new lung injury treatment scheme based on natural active compounds is provided, and the piperlongumine can be widely applied to prevention and treatment of acute lung injury and related inflammatory lung diseases.

The invention discloses firewood-raisin tree bark and pinellia ternate granules as well as a preparation method and application thereof, and relates to the technical field of traditional Chinese medicines. The granules are prepared from seven traditional Chinese medicinal materials including radix bupleuri, radix scutellariae, fructus aurantii, ginger processed pinellia, pericarpium trichosanthis, radix paeoniae rubra and platycodon grandiflorum according to a specific weight ratio, and through a synergistic effect, the effects of soothing the liver, regulating qi, clearing heat, reducing phlegm, relieving chest stuffiness and removing stasis are achieved. The preparation method mainly comprises the following steps: repeatedly decocting and extracting with water, merging filtrate, concentrating under reduced pressure to prepare clear paste, drying, adding a proper amount of diluent, adhesive and corrigent, granulating and sub-packaging. The preparation process is scientific and reasonable, has good reproducibility, is easy to realize standardized and industrial mass production, and can effectively ensure the stability and uniformity of batch-to-batch quality. The traditional Chinese medicine composition can effectively relieve symptoms such as chest pain, cough and dyspnea, promotes pleural effusion absorption, is safe in clinical application, and provides a new and effective traditional Chinese medicine choice for treatment of tuberculous pleurisy.

The invention belongs to the technical field of biological medicines, and discloses a single-domain antibody for detecting CD28, and the amino acid sequence of the single-domain antibody is as shown in SEQ ID No.1. The antibody has good reaction activity with human CD28, can be used for detecting or diagnosing CD28 and treating related diseases with abnormal expression of CD28, and provides a basis for research and development of drugs targeting CD28.

The application discloses application of an sEH inhibitor in preparation of a medicine for treating IgA nephropathy. The application proposes that an sEH inhibitor (such as t-AUCB, N-benzyl linoleamide) is used to inhibit sEH activity, restore the level of endogenous anti-inflammatory mediator epoxyeicosatrienoic acid (EETs), and rebuild the EETs / DiHETs metabolic balance. Experiments prove that the sEH inhibitor can significantly reduce the urine albumin / creatinine ratio of an IgA nephropathy model mouse, improve the blood urea and creatinine levels, and reduce glomerular IgA deposition. Mechanically, the therapy can restore the EETs level, down-regulate the content of hydrolysis product DiHETs, rebuild the EETs / DiHETs metabolic balance, and then inhibit the expression of renal IL-1beta, IL-6, TNF-alpha and other pro-inflammatory factors. It is clear that the sEH / EET axis is a key pathogenic pathway of IgA nephropathy, and a novel metabolic targeting strategy is provided for developing a specific medicine for treating IgA nephropathy.

This invention discloses an intelligent programmed engineered probiotic system and its preparation method. The system uses probiotic strains as the engineered substrate bacteria, and is constructed by integrating the coding genes for superoxide dismutase (SOD), antimicrobial peptides (AMP), and a synchronized cleavage circuit (SLC) with the probiotic strains through genetic engineering technology. This invention also discloses a pH-responsive genetically engineered bacterial microsphere containing the aforementioned intelligent programmed engineered probiotic system, which is encapsulated in a carrier formed by the cross-linking reaction of sodium alginate and calcium chloride ions. The intelligent programmed engineered probiotic system and the accompanying pH-responsive genetically engineered bacterial microspheres constructed in this invention, through the synergistic design of the dual-function therapeutic modules SOD and AMP, and the programmed cleavage circuit SLC, simultaneously address the three core problems of IBD: oxidative stress, dysbiosis, and intestinal barrier damage.

The invention relates to the technical field of biological medicine, and particularly discloses a preparation method of a collagen and anti-propionibacterium acnes gamma protein combined preparation, and the preparation method of the collagen and anti-propionibacterium acnes gamma protein combined preparation comprises the following steps: preparing anti-propionibacterium acnes gamma protein; adding water into the anti-propionibacterium acnes gamma protein and the recombinant III-type collagen, stirring and mixing, adding glycerol and a neutralizing agent, and mixing to obtain a uniform solution; the preparation method comprises the following steps: dispersing carbomer with water, adding a preservative and a humectant, mixing, finally adding the uniform solution, and mixing to obtain the required collagen and anti-propionibacterium acnes gamma protein combined preparation. The combined preparation prepared by the method can be used for effectively treating acnes, is remarkable in curative effect, does not generate drug resistance, is short in treatment cycle, can quickly eliminate skin damage, promote cell growth and adhesion and repair skin barriers, and does not generate new rash after healing.

This invention provides a circRNA-LNP delivery system for in vivo induction of iTregs, its preparation method, and its applications. The delivery system includes circRNA encoding human TGF-β1, lipid nanoparticles encapsulating the circRNA, and IL-2 modified on the surface of the lipid nanoparticles. This invention synergistically combines retinoic acid as a functional immunomodulatory small molecule with five lipids, significantly improving the uptake efficiency of Treg precursor cells and the induction efficiency of iTregs. It can also efficiently increase the proportion of functional iTregs in diseased tissues in vivo, demonstrating excellent therapeutic effects on immunomodulatory diseases such as ulcerative colitis, and providing a novel technical pathway for the radical treatment of autoimmune and inflammatory diseases.