2620 results about "FluoProbes" patented technology

The invention provides a method and a system for evaluating the luminescence performance of a near-infrared two-region molecular fluorescent probe, and relates to the technical field of fluorescent probes, the method comprises the following steps: obtaining the initial configuration of the near-infrared two-region molecular fluorescent probe in an aqueous solution, and carrying out molecular dynamics simulation on the initial configuration to obtain a plurality of representative conformations of the probe in the aqueous solution; performing quantum chemical calculation on each representative conformation to obtain first system energy of each representative conformation in a stable state and second system energy and intensity parameters of each representative conformation in a lowest singlet excitation state; generating a comprehensive evaluation factor based on the first system energy, the second system energy and the intensity parameter; and sorting the luminescence properties of the probes based on the comprehensive evaluation factor, and screening out the probe with the optimal performance. According to the method provided by the invention, quantitative evaluation on the luminescence property of the near-infrared two-region molecular fluorescent probe is realized, and technical support is provided for optimal design and clinical application of the probe.

The invention discloses an AIE type fluorescent probe with tumor targeting as well as preparation and application thereof, and belongs to the technical field of medicines, the probe can accurately target HDAC6 high-expression tumor cells, the specific binding capacity of the probe reduces interference to normal cells, and the detection accuracy is improved. The probe is fused with AIE performance dye molecules and emits strong fluorescence during aggregation, so that the imaging sensitivity and definition are remarkably enhanced. By optimizing the structure and synthesis conditions, the fluorescence intensity and stability of the probe are improved, the sensitivity of early tumor detection is ensured, and the survival rate and life quality of patients are improved. The preparation method is simple, convenient, feasible, low in cost and beneficial to practical application. The probe can be used for preparing tumor detection products, assisting in tumor diagnosis and classification, monitoring treatment changes and evaluating the curative effect. High-precision imaging can be realized in the aspect of tumor imaging, the method can be used for evaluating curative effect and guiding formulation of subsequent treatment schemes, and the application prospect is wide.

The invention provides an intracellular mycobacterium tuberculosis in-situ nucleic acid detection method based on CRISPR / Cas and application, and the method comprises the following steps: S1, designing crRNA and a primer pair of a mycobacterium tuberculosis target gene sequence, and synthesizing a single-stranded DNA fluorescent probe; s2, preparing a standard solution containing a target gene sequence, and incubating with a CRISPR / Cas12a system; s3, extracting nucleic acids of different types of bacteria, uniformly mixing the nucleic acids of different types of bacteria with the CRISPR / Cas12a system, and incubating; s4, incubating an attenuated strain of mycobacterium tuberculosis and the DNA fluorescent probe, infecting macrophages, and incubating to obtain infected cells; s5, electrically transferring a CRISPR / Cas12a system into the infected cells in S4 by utilizing an electroporation technology, incubating, and detecting the intensity of a fluorescence signal; and S6, performing quantitative and positioning analysis on the detected fluorescence signal to complete detection, and directly introducing a CRISPR / Cas12a system into the macrophage by adopting an electroporation technology to realize intracellular in-situ detection, thereby avoiding the operation complexity of a nucleic acid extraction link and the loss of detection sensitivity in the prior art.

The invention relates to the technical field of biogenic amine detection, in particular to a dicyanoethylene-based fluorescent material as well as a preparation method and application thereof. After the biogenic amine is in contact with the glass surface fluorescent probe, due to the nucleophilic characteristic of the biogenic amine, the biogenic amine can be subjected to nucleophilic substitution reaction with a probe molecule dicyanovinyl group to generate an imine chemical structure. As the electron-withdrawing capability of dicyanoethylene is stronger than that of imine, the intramolecular charge transfer effect of probe molecules is greatly reduced, so that blue shift of absorption and luminescence properties of the probe molecules is caused, and after the fluorescent probe is loaded on the surface of glass, the fluorescence intensity of the fluorescent probe is improved. The formed dicyanoethylene-based fluorescent material provides a solution for the technical problem that the existing biogenic amine detection mode is inconvenient.

The invention provides a kit and a method for detecting common fusion genes of lymphoid leukemia based on a multiple digital PCR (Polymerase Chain Reaction) method. By constructing a synergistic effect mechanism of the primer and the fluorescent probe and optimizing matching of the primer, the probe and a fluorescent marker, non-specific binding among amplification products is effectively avoided. A composite reaction aid with a specific formula, namely a PCR enhancer, is innovatively introduced, so that the amplification efficiency and specificity of a multiple detection system are remarkably improved. According to the kit provided by the invention, the integrated detection capability on seven fluorescent channels, namely Atto 425, VIC, FAM, ROX, CY5, CY5.5 and CY7, is realized in a single-hole design. By innovatively constructing an ROX + CY5 and Atto425 + VIC dual-channel joint detection system, when the ROX and CY5 channels or Atto425 and VIC channels in the same detection hole simultaneously present positive signals, two additional detection targets can be specifically interpreted. The innovative detection strategy based on channel combination enables the kit to realize precise detection of 18 fusion genes only through two detection holes in a breakthrough manner.

The invention provides an NIR-IIb / c rare earth nanoparticle fluorescent probe as well as a preparation method and application thereof. The fluorescent probe sequentially comprises a core, an active shell and an inert shell from inside to outside, wherein the core comprises Tm < 3 + > ions and Er < 3 + > ions, and the active shell comprises Er < 3 + > ions, Yb < 3 + > ions and Ce < 3 + > ions. The fluorescent probe provided by the invention has an efficient energy transfer system, not only has bright luminescence in an NIR-IIb wave band (1500-1700 nm), but also has luminous intensity increased by dozens of times in an NIR-IIc wave band (1700-1900 nm) compared with that of a traditional probe. The hydrophilic modification method of the probe is also improved, and the biocompatibility of the probe is improved by a multi-layer polymer cross coating method, so that the probe still has good luminescence property in an organism, and the penetration depth and resolution of biological tissues during imaging are improved.

The invention discloses a cathepsin B activated fluorescent probe which comprises a dicyanoisosulfone fluorescent report unit and a cathepsin B specific restriction enzyme cutting site GFLG peptide chain. When CTSB specifically cuts a GFLG peptide chain, a DCO molecule with an amino group with relatively strong electron donating ability is generated, so that the fluorescence emission behavior of the probe is rapidly activated through a one-step enzyme catalysis reaction. The invention also provides a preparation method of the cathepsin B activated fluorescent probe. The invention also provides a nano particle for packaging the cathepsin B activated fluorescent probe, and the nano particle is simple in preparation process and mild in reaction condition. The cathepsin B activated fluorescent probe is prepared into nanoparticles, and the nanoparticles can realize accumulation of the material in tumor tissues and accurate imaging in apoptotic tumor cells by enhancing the permeation retention effect, have better biocompatibility and can be used for detecting the apoptotic tumor cells.

The invention discloses a carbon quantum dot / metal organic framework composite material as well as a preparation method and application thereof, and the method is characterized in that carbon quantum dots derived from waterborne polyurethane are embedded into a zeolite imidazate framework 8 to prepare the carbon quantum dot / metal organic framework composite material. The composite material can be used as a ratio type fluorescent probe for trace detection of tetracycline, a dual-emission fluorescence signal of the probe is derived from tetracycline which quenches blue fluorescence of puCDs at 450 nm through an inner filter effect, and the coordination effect between Zn < 2 + > and tetracycline triggers an aggregation-induced emission effect, so that new fluorescence emission is generated, and the detection sensitivity is improved. The intensity of the two signals changes in proportion to the concentration of tetracycline, the wide linear concentration detection range and the low detection limit are achieved, the probe has the high recovery rate and the large adsorption capacity in detection, the problems that a traditional detection method is insufficient in sensitivity, poor in anti-interference capacity, high in cost and the like are solved, and the application prospect is wide. A new thought is provided for constructing a ratio type fluorescent probe with a removal function.

The invention discloses a copper (II) coordination type naphthalimide fluorescent probe and a preparation method and application thereof in pasture detection, and relates to the field of fluorescent probes, the preparation method of the fluorescent probe comprises the following steps: (1) preparation of an intermediate a: preparing the intermediate a by using 2-bromo-1, 8-naphthalic anhydride; (2) preparing an intermediate b: preparing the intermediate b by using the intermediate a; (3) preparing an intermediate c: preparing the intermediate c by using the intermediate b; (4) preparing Nap2: preparing Nap2 by using the intermediate c; the raw materials for preparing the Nap2 are easy to obtain, the synthesis steps are simple, the operation is simple and convenient, and the Nap2 has high application value in the field of rapid detection of pasture copper pollution.

The invention relates to the technical field of fluorescent probes, and provides a low-concentration ratio type high-concentration enhanced fluorescent probe as well as a preparation method and application thereof in order to solve the problems that a traditional fluorescent probe depends on a high-concentration use condition and is often faced with fast probe consumption, increased biotoxicity, non-specific target binding and the like in a biological sample. The preparation method of the fluorescent probe comprises the following steps: step 1, preparing phenothiazine dialdehyde; and 2, mixing phenothiazine dialdehyde, malononitrile, ethanol, acetonitrile, DMF and piperidine, and carrying out a reflux reaction to obtain the low-concentration ratio-type high-concentration enhanced fluorescent probe. The probe provided by the invention realizes quantitative detection of HClO through synchronous change of two emission channels, and the detection sensitivity and reliability are remarkably improved; when the concentration is high, an enhanced single-channel fluorescence amplification effect is shown, and strong signal identification and macroscopic visual imaging in specific application are facilitated, so that the dual requirements of trace monitoring and high-throughput screening are met.

The invention belongs to the technical field of specific fluorescent probe detection, and particularly relates to a near-infrared fluorescent probe for detecting hydrazine hydrate as well as a preparation method and application of the near-infrared fluorescent probe. The structural formula of the near-infrared fluorescent probe is # imgabs0. The invention provides a preparation method for synthesizing the probe by taking xanthene dye and acetyl chloride as raw materials. By researching the fluorescence spectrum of the fluorescent probe, the selectivity of the probe on N2H4 is evaluated, the fluorescence intensity of the probe is determined to be obviously enhanced only in the presence of N2H4, and the fluorescence intensity is positively correlated with the concentration of N2H4; meanwhile, the probe has the characteristics of quick response and near-infrared emission; the fluorescent probe disclosed by the invention has relatively small cytotoxicity and can be applied to detection of HepG2 cell exogenous hydrazine hydrate.

The invention relates to a coumarin-based fluorescent probe as well as a preparation method and application thereof in the aspect of HClO detection, and belongs to the technical field of HClO detection. The invention discloses a coumarin-based fluorescent probe W (O-(3-((2-morpholinyl ethyl) carbamoyl)-2-oxo-2hydrogen-benzopyran-7-yl) dimethyl thiocarbamate) prepared on the basis of an intramolecular charge transfer (ICT) mechanism, and a preparation method of the coumarin-based fluorescent probe W. The novel lysosome targeting fluorescent probe contains coumarin as a fluorophore, a morpholine group as a targeting group and a dimethyl thiocarbamate group as a recognition group, is a novel lysosome targeting fluorescent probe, and has excellent performances of high selectivity, low detection limit, low cytotoxicity, high resolution and the like in the aspect of HClO detection; the method can be used for detecting the content of endogenous and exogenous hypochlorous acid in living cells.

The invention discloses a detection kit and method for detecting allergic inflammatory factors based on a centrifugal micro-fluidic chip, and relates to the field of kits, the detection kit comprises the centrifugal micro-fluidic chip and a preset reagent; the centrifugal micro-fluidic chip comprises a chip base body, a sample adding cavity, a liquid level sensor, a liquid level sensor, a liquid level sensor, a liquid level sensor and a liquid level sensor, the nucleic acid extraction and purification cavity comprises a plurality of amplification cavities; a reagent storage chamber; a waste liquid chamber; and a capillary valve. The functions of nucleic acid extraction, variable temperature amplification and multi-target synchronous detection are integrated through the centrifugal micro-fluidic chip, full-closed operation is achieved by means of centrifugal force or other driving force such as air pressure and electroosmotic flow, and the pollution risk caused by manual step-by-step operation is thoroughly eliminated; a multi-wavelength distinguishable fluorescent probe and a grouping preset primer combination are adopted to synchronously complete parallel detection of the allergic inflammatory factor mRNA and the drug accompanying diagnosis target, cross interference is avoided, and large instruments and equipment do not need to be relied on.

The invention relates to application of a heterocyclic compound as a fluorescent probe. The structural formula of the heterocyclic compound is shown as a general formula I, and R1, R2, R3, R4, R5, R6, R7 and R8 are independently one of hydrogen, alkyl, hydroxyl, phenyl, hydroxyphenyl, indole and oxymethyl. According to the present invention, the heterocyclic compounds represented by the general formula I can be specifically positioned in mitochondria, and have long fluorescence lifetime, such that the heterocyclic compounds are suitable for being used as mitochondria fluorescence probes.

The invention provides a fluorescent probe for detecting hydroxyl radicals as well as a preparation method and application thereof, and the preparation method comprises the following steps: (S10) synthesizing a first intermediate compound by taking 2, 3, 3-trimethyl-3H-indoline and methyl iodide as raw materials; (S20) synthesizing a second intermediate compound by taking phosphorus trichloride, cyclohexanone and anhydrous dimethylformamide as raw materials; (S30) mixing and reacting the first intermediate compound and the second intermediate compound to synthesize a compound IR780; and (S40) synthesizing OH-probe on the basis of the IR780. The prepared OH-probe has extremely high sensitivity and excellent selectivity on hydroxyl radicals, can be effectively used for monitoring and mapping the level of the hydroxyl radicals, and can be applied to detection of atherosclerosis, obesity, cancers, neurodegenerative diseases, inflammatory diseases and other free radical related diseases. Wide development prospects and practical application values are realized.

The present application discloses methods for characterising vesicles. The method involves (1) a sample preparation step, comprising providing a test specimen with vesicles attached to a substrate, wherein the vesicles are labelled with one or more fluorescent probes; (2) an image acquisition step, comprising imaging said one or more fluorescent probes on the vesicles to generate image data; (3) an image processing step which identifies individual vesicles and constructs a feature vector containing characterising parameters for individual vesicles characterising parameters (including a morphological parameter) (4) a data transformation step to calculate modified feature vectors of lower dimensionality for individual vesicles; and (5) a characterisation step, which characterises the vesicles based on the modified feature vectors. The application also discloses methods for immobilising vesicles on a substrate, as well as substrates functionalised to capture vesicles.

The invention discloses a ratio-type fluorescence analysis method for determining sodium dehydroacetate, which comprises the following steps: under an alkaline condition, synthesizing tetrafluoroterephthalic acid oxide, namely a fluorescent substance TFAox, by oxidizing tetrafluoroterephthalic acid with hydrogen peroxide, and forming a ratio-type fluorescence probe by the fluorescent substance and rhodamine B, a ratio-type fluorescence analysis method of sodium dehydroacetate is constructed based on the ratio-type fluorescence probe, and the method can be successfully applied to determination of sodium dehydroacetate in a water sample and a bread sample. The ratio-dependent fluorescence detection method for determining sodium dehydroacetate provided by the invention has the characteristics of wide linear range, high detection sensitivity, no interference of coexisting substances on determination, good selectivity and the like.

The invention belongs to the field of fluorescence detection, and particularly relates to a ratio-type fluorescent nanoprobe for specifically detecting tetracycline antibiotics as well as a preparation method and an application method of the ratio-type fluorescent nanoprobe. The nano-probe is a europium modified zinc gallium germanate nano-material, and is prepared by amino modification, citric acid modification and europium modification of the zinc gallium germanate nano-material. The probe can be specifically combined with tetracycline antibiotics; the fluorescent probe has two different fluorescence emission intensities, so that interference can be effectively eliminated, and the detection accuracy is high. And the synthesis method is simple and low in cost. The ratio type fluorescent nanoprobe provided by the invention also can realize the change of different colors under an ultraviolet lamp, can realize quantitative visual detection of tetracycline by combining the RGB value and the tetracycline concentration change, and can realize rapid on-site detection of tetracycline in a sample without the help of a fluorescent instrument. The tetracycline fluorescent probe is stable in optical performance, not prone to photobleaching, high in tetracycline recognition capacity, wide in response range (0.2-70 [mu] M) and low in detection limit, and has remarkable industrial application potential.

The invention discloses a TaqMan real-time fluorescent quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection fluorescent probe and kit for corn necrosis stripe virus and application, and relates to the technical field of virus detection. The nucleotide sequence of the fluorescent probe provided by the invention is 5 '-FAM-CGCTGGTACATAGGATGGCAAC-TAMRA-3', and the nucleotide sequence of the fluorescent probe provided by the invention is shown in the description. The nucleotide sequence of the primer is as follows: an upstream primer: 5 '-CCTGCTCCTCTGCTATTCG-3'; a downstream primer: 5 '- The downstream primer is 5 '-CAGACTTCCCTTGGCAGTGT-3', and the downstream primer is 5 '- The kit at least comprises the fluorescent probe and the primer. The TaqMan real-time fluorescent quantitative RT-PCR detection method established by the invention is simple and convenient to operate, strong in specificity, high in sensitivity and good in repeatability, is not only suitable for qualitative identification, but also can realize accurate quantitative analysis; the sensitivity is at least 100 times higher than that of a common RT-PCR detection method. The problem that in the prior art, a TaqMan real-time fluorescent quantitative RT-PCR detection method for detecting the corn necrotic stripe virus is lacked can be solved.

The invention belongs to the field of biological medicine, and provides a preparation method and application of a fluorescent probe molecule. The synthesized fluorescent probe molecule can be combined with an intramolecular fluorescence quenching principle to realize real-time monitoring of drug molecule release of the polypeptide drug conjugate in serum, and can also realize tracing of polypeptide in the polypeptide drug conjugate at a cellular level and monitoring of drug molecule release and dynamic distribution. The comprehensive visual research on the hybrid peptide is realized. The preparation method of the fluorescent probe is simple and easy to implement. The method provides an effective visual tool molecule for research and development of the polypeptide drug conjugate. The monitoring method disclosed by the invention is relatively simple and convenient to operate, good in monitoring result stability, strong in monitoring capability and mild in condition, provides an efficient tool molecule for development of the polypeptide drug conjugate, and has relatively high practical application value.

The invention discloses an organelle-targeted fluorescent probe for specific detection of tumor cells as well as preparation and application of the organelle-targeted fluorescent probe, and the specific structural formula of the fluorescent probe is as follows: BCY-DP shows obvious specific targeting ability of tumor cell mitochondria and has been applied to ONOO-selective imaging in living cell mitochondria; results show that the BCY-DP has high selectivity on ONOO <-> in tumor cells, can successfully distinguish the ovarian cancer cells from normal ovarian cells, and can be used for diagnosis of cancer cells.

The invention relates to the technical field of biological medicines, and particularly discloses a BODIPY (boron dipyrromethene)-based compound and an application of the BODIPY-based compound in photodynamic oxidation of beta-amyloid protein (Abeta). The compound takes iodo-BODIPY as a photosensitive core and is connected with 4-t-butyloxycarboryl benzene as an A beta recognition group. The introduction of iodine atoms significantly enhances the intersystem crossing ability of molecules, so that the molecules have excellent reactive oxygen species (ROS) generation ability. The compound has high ROS yield, good optical stability and biocompatibility, can specifically target A beta aggregate, realizes photodynamic oxidative degradation, and provides a novel potential tool for treatment of Alzheimer's disease.

The invention relates to the technical field of molecular biology, in particular to a qPCR (quantitative polymerase chain reaction) detection kit for detecting cis-trans mutation and a use method of the qPCR detection kit. The invention provides a primer pair which is used for detecting a first mutation site and / or a second mutation site of a target gene and comprises a universal primer F matched with the upstream of the first mutation site, a downstream primer R-BW matched with a wild type sequence of the second mutation site and a downstream primer R-BM matched with a mutation type sequence of the second mutation site, the fluorescent probe P-AW is matched with the upstream of the first mutation site, the fluorescent probe P-AM is matched with the mutant sequence of the first mutation site, and the fluorescent groups of the fluorescent probe P-AW and the fluorescent probe P-AM are different. According to the reaction system disclosed by the invention, the MGB fluorescent probe and the ARMS primer are combined, and mutation information and a cis-trans relationship can be judged according to the existence of a fluorescent signal. Compared with an existing detection method, the method for detecting the cis-trans relation through fluorescent quantitative PCR is proposed for the first time, detection can be completed within 2 h, specificity is good, the cost advantage is achieved, operation is easy and convenient, and clinical operation is convenient.

The invention belongs to the field of fluorescence monitoring, and particularly relates to a preparation method of a fluorescent probe for monitoring chloride ions in a cement base and detection equipment, and the preparation method comprises preparation of sodium alginate fluorescent nanoparticles, preparation of a cellulose acetate membrane solution and preparation of the fluorescent probe. The prepared sensor is excellent in performance, the detection range of chloride ions in a cement matrix reaches 0.001-0.3 M, the average measurement error is only 1.4%, the single measurement error is smaller than or equal to 3%, the single measurement time is only 6 seconds, and in-situ, rapid and high-precision monitoring of the chloride ions is achieved. The invention provides an effective new technical means for concrete structure durability monitoring, and has important engineering application value.

The invention relates to a fluorescent probe for detecting negative electrode lithium precipitation in a lithium ion battery and a detection method, and belongs to the technical field of lithium ion batteries. The fluorescent probe comprises a complex formed by coordination combination of 8-hydroxyquinoline-5-sulfonic acid and metal ions, the complex emits fluorescence under excitation of ultraviolet light, and the standard electrode potential of a metal simple substance corresponding to the metal ions is higher than that of lithium. The fluorescent probe can rapidly, simply and sensitively realize qualitative and quantitative detection of negative electrode lithium precipitation, and compared with large-scale instruments and visual inspection, the fluorescent probe can effectively simplify operation, reduce cost and improve detection efficiency and accuracy, meanwhile, a battery does not need to be disassembled under the condition of full charge, and detection safety is improved.

The invention provides application of nano CDs as a fluorescent probe in fluorescence detection of sugammadex sodium and a fluorescence detection method of sugammadex sodium, and relates to the technical field of drug analysis and detection. According to the invention, carboxyl in sugammadex sodium and nitrogen atoms in nano CDs interact with each other, the fluorescence intensity of nano CDs is enhanced through intramolecular charge transfer, sugammadex sodium and nano CDs can be effectively combined through a static combination effect, and nano CDs have high sensitivity and selectivity to sugammadex sodium; the method is suitable for detecting sugammadex sodium. By adopting a fluorescence analysis method, the high-sensitivity, high-selectivity, simple, rapid and accurate quantitative detection of the sugammadex can be realized according to the fluorescence enhancement efficiency of the sugammadex relative to the nano CDs after the sugammadex is added and a standard curve of the concentration of the sugammadex and the fluorescence enhancement efficiency. The fluorescence detection method provided by the invention is low in detection cost.

The invention discloses a moisture fluorescence probe based on a D-pi-A structure as well as a preparation method and application of the moisture fluorescence probe. The preparation method specifically comprises the following steps: dissolving WP in a solvent, reacting with BF3. Et2O under the protection of nitrogen, filtering, washing and drying to obtain a red target product WP-BF3. According to the present invention, the change of the microenvironment moisture content can be responded, the significant fluorescence signal response can be generated at the 487 nm position, the quantitative analysis detection of the moisture content in the organic solvent and the food sample can be achieved, the response is rapid, the sensitivity is good, and the accuracy is high.

The invention discloses an ether chain modified rhodamine fluorescent dye, an ether chain modified rhodamine fluorescent dye probe and a preparation method and application thereof, through modification of an ether chain, the water solubility of the rhodamine dye is improved, the aggregation quenching effect is reduced, meanwhile, the intramolecular torsional charge transfer (TICT) is reduced through the electron withdrawing effect of oxygen atoms, and the fluorescence sensitivity is improved. Finally, the quantum yield of the rhodamine dye is greatly improved. The quantum yield of ether chain modified oxygen rhodamine water is increased from 0.31 to 0.70, and the quantum yield of silicon-based rhodamine water is increased from 0.19 to 0.41. The rhodamine dye disclosed by the invention has the characteristics of high brightness, easiness in functional derivation and the like, and has application prospects in the fields of fluorescent probes, fluorescent labels, super-resolution fluorescence imaging and the like.

The invention provides a novel D-A-D type fluorescent probe material as well as a preparation method and application thereof. The fluorescent probe material has good stability, the preparation process is simple, and the synthesis condition is mild. Moreover, the D-A-D type fluorescent probe material has good solubility and stability, and a complex of the D-A-D type fluorescent probe material and zinc ions can identify pyrophosphate ions with high selectivity and high sensitivity. The interference of phosphate ions, hydrogen phosphate ions, dihydrogen phosphate ions, fluorine ions, chloride ions, nitrate ions, acetate ions, sulfate ions and carbonate ions is avoided; after the zinc ion complex of the D-A-D type fluorescent probe material is combined with PPi, the fluorescence intensity is remarkably enhanced, the D-A-D type fluorescent probe material has a higher signal-to-noise ratio, in-situ imaging detection of a target object in living cells or tissues is realized, the defects of a traditional molecule and ion detection method are overcome, and the D-A-D type fluorescent probe material is suitable for being widely applied to the field of biological imaging. # imgabs0 #

The invention discloses a mercury ion detection fluorescent probe based on quinoline as well as a preparation method and application of the mercury ion detection fluorescent probe. The fluorescent probe and a structural formula of the fluorescent probe are shown as a formula I in the specification. A quinoline derivative, namely 2-methyl-7-hydroxyquinoline, is used as a fluorescent chromophore, a thioester group is used as a recognition group, and the prepared fluorescent probe shows high sensitivity and high selectivity on Hg < 2 + > in a solution. The preparation method of the fluorescent probe is simple in steps, raw materials are easy to obtain, and the obtained product is a white flocculent solid, is easy to store, has a wide application prospect and can be produced and applied on a large scale.