1454 results about "FluoProbes" patented technology

The invention discloses a carbon quantum dot / metal organic framework composite material as well as a preparation method and application thereof, and the method is characterized in that carbon quantum dots derived from waterborne polyurethane are embedded into a zeolite imidazate framework 8 to prepare the carbon quantum dot / metal organic framework composite material. The composite material can be used as a ratio type fluorescent probe for trace detection of tetracycline, a dual-emission fluorescence signal of the probe is derived from tetracycline which quenches blue fluorescence of puCDs at 450 nm through an inner filter effect, and the coordination effect between Zn < 2 + > and tetracycline triggers an aggregation-induced emission effect, so that new fluorescence emission is generated, and the detection sensitivity is improved. The intensity of the two signals changes in proportion to the concentration of tetracycline, the wide linear concentration detection range and the low detection limit are achieved, the probe has the high recovery rate and the large adsorption capacity in detection, the problems that a traditional detection method is insufficient in sensitivity, poor in anti-interference capacity, high in cost and the like are solved, and the application prospect is wide. A new thought is provided for constructing a ratio type fluorescent probe with a removal function.

The invention discloses a detection kit and method for detecting allergic inflammatory factors based on a centrifugal micro-fluidic chip, and relates to the field of kits, the detection kit comprises the centrifugal micro-fluidic chip and a preset reagent; the centrifugal micro-fluidic chip comprises a chip base body, a sample adding cavity, a liquid level sensor, a liquid level sensor, a liquid level sensor, a liquid level sensor and a liquid level sensor, the nucleic acid extraction and purification cavity comprises a plurality of amplification cavities; a reagent storage chamber; a waste liquid chamber; and a capillary valve. The functions of nucleic acid extraction, variable temperature amplification and multi-target synchronous detection are integrated through the centrifugal micro-fluidic chip, full-closed operation is achieved by means of centrifugal force or other driving force such as air pressure and electroosmotic flow, and the pollution risk caused by manual step-by-step operation is thoroughly eliminated; a multi-wavelength distinguishable fluorescent probe and a grouping preset primer combination are adopted to synchronously complete parallel detection of the allergic inflammatory factor mRNA and the drug accompanying diagnosis target, cross interference is avoided, and large instruments and equipment do not need to be relied on.

The invention relates to application of a heterocyclic compound as a fluorescent probe. The structural formula of the heterocyclic compound is shown as a general formula I, and R1, R2, R3, R4, R5, R6, R7 and R8 are independently one of hydrogen, alkyl, hydroxyl, phenyl, hydroxyphenyl, indole and oxymethyl. According to the present invention, the heterocyclic compounds represented by the general formula I can be specifically positioned in mitochondria, and have long fluorescence lifetime, such that the heterocyclic compounds are suitable for being used as mitochondria fluorescence probes.

The invention discloses a ratio-type fluorescence analysis method for determining sodium dehydroacetate, which comprises the following steps: under an alkaline condition, synthesizing tetrafluoroterephthalic acid oxide, namely a fluorescent substance TFAox, by oxidizing tetrafluoroterephthalic acid with hydrogen peroxide, and forming a ratio-type fluorescence probe by the fluorescent substance and rhodamine B, a ratio-type fluorescence analysis method of sodium dehydroacetate is constructed based on the ratio-type fluorescence probe, and the method can be successfully applied to determination of sodium dehydroacetate in a water sample and a bread sample. The ratio-dependent fluorescence detection method for determining sodium dehydroacetate provided by the invention has the characteristics of wide linear range, high detection sensitivity, no interference of coexisting substances on determination, good selectivity and the like.

The invention belongs to the field of biological medicine, and provides a preparation method and application of a fluorescent probe molecule. The synthesized fluorescent probe molecule can be combined with an intramolecular fluorescence quenching principle to realize real-time monitoring of drug molecule release of the polypeptide drug conjugate in serum, and can also realize tracing of polypeptide in the polypeptide drug conjugate at a cellular level and monitoring of drug molecule release and dynamic distribution. The comprehensive visual research on the hybrid peptide is realized. The preparation method of the fluorescent probe is simple and easy to implement. The method provides an effective visual tool molecule for research and development of the polypeptide drug conjugate. The monitoring method disclosed by the invention is relatively simple and convenient to operate, good in monitoring result stability, strong in monitoring capability and mild in condition, provides an efficient tool molecule for development of the polypeptide drug conjugate, and has relatively high practical application value.

The invention discloses an organelle-targeted fluorescent probe for specific detection of tumor cells as well as preparation and application of the organelle-targeted fluorescent probe, and the specific structural formula of the fluorescent probe is as follows: BCY-DP shows obvious specific targeting ability of tumor cell mitochondria and has been applied to ONOO-selective imaging in living cell mitochondria; results show that the BCY-DP has high selectivity on ONOO <-> in tumor cells, can successfully distinguish the ovarian cancer cells from normal ovarian cells, and can be used for diagnosis of cancer cells.

The invention relates to the technical field of biological medicines, and particularly discloses a BODIPY (boron dipyrromethene)-based compound and an application of the BODIPY-based compound in photodynamic oxidation of beta-amyloid protein (Abeta). The compound takes iodo-BODIPY as a photosensitive core and is connected with 4-t-butyloxycarboryl benzene as an A beta recognition group. The introduction of iodine atoms significantly enhances the intersystem crossing ability of molecules, so that the molecules have excellent reactive oxygen species (ROS) generation ability. The compound has high ROS yield, good optical stability and biocompatibility, can specifically target A beta aggregate, realizes photodynamic oxidative degradation, and provides a novel potential tool for treatment of Alzheimer's disease.

The invention relates to the technical field of molecular biology, in particular to a qPCR (quantitative polymerase chain reaction) detection kit for detecting cis-trans mutation and a use method of the qPCR detection kit. The invention provides a primer pair which is used for detecting a first mutation site and / or a second mutation site of a target gene and comprises a universal primer F matched with the upstream of the first mutation site, a downstream primer R-BW matched with a wild type sequence of the second mutation site and a downstream primer R-BM matched with a mutation type sequence of the second mutation site, the fluorescent probe P-AW is matched with the upstream of the first mutation site, the fluorescent probe P-AM is matched with the mutant sequence of the first mutation site, and the fluorescent groups of the fluorescent probe P-AW and the fluorescent probe P-AM are different. According to the reaction system disclosed by the invention, the MGB fluorescent probe and the ARMS primer are combined, and mutation information and a cis-trans relationship can be judged according to the existence of a fluorescent signal. Compared with an existing detection method, the method for detecting the cis-trans relation through fluorescent quantitative PCR is proposed for the first time, detection can be completed within 2 h, specificity is good, the cost advantage is achieved, operation is easy and convenient, and clinical operation is convenient.

The invention discloses an ether chain modified rhodamine fluorescent dye, an ether chain modified rhodamine fluorescent dye probe and a preparation method and application thereof, through modification of an ether chain, the water solubility of the rhodamine dye is improved, the aggregation quenching effect is reduced, meanwhile, the intramolecular torsional charge transfer (TICT) is reduced through the electron withdrawing effect of oxygen atoms, and the fluorescence sensitivity is improved. Finally, the quantum yield of the rhodamine dye is greatly improved. The quantum yield of ether chain modified oxygen rhodamine water is increased from 0.31 to 0.70, and the quantum yield of silicon-based rhodamine water is increased from 0.19 to 0.41. The rhodamine dye disclosed by the invention has the characteristics of high brightness, easiness in functional derivation and the like, and has application prospects in the fields of fluorescent probes, fluorescent labels, super-resolution fluorescence imaging and the like.

The invention discloses a mercury ion detection fluorescent probe based on quinoline as well as a preparation method and application of the mercury ion detection fluorescent probe. The fluorescent probe and a structural formula of the fluorescent probe are shown as a formula I in the specification. A quinoline derivative, namely 2-methyl-7-hydroxyquinoline, is used as a fluorescent chromophore, a thioester group is used as a recognition group, and the prepared fluorescent probe shows high sensitivity and high selectivity on Hg < 2 + > in a solution. The preparation method of the fluorescent probe is simple in steps, raw materials are easy to obtain, and the obtained product is a white flocculent solid, is easy to store, has a wide application prospect and can be produced and applied on a large scale.

The invention belongs to the technical field of iron ion detection, and particularly relates to a rapid detection method for ferric ions in water, which comprises the following steps: a fluorescent probe preparation step: taking trihydrate of copper nitrate and tryptamine, adding into a centrifuge tube in sequence, adding ultrapure water, performing ultrasonic dissolution, transferring into a high-pressure reaction kettle, and performing hydrothermal reaction to obtain a fluorescent probe; cooling to room temperature, centrifuging the obtained yellow green solution in a centrifugal machine, and filtering the supernate through a microfiltration membrane to obtain a copper-doped carbon quantum dot CuCDs stock solution, namely the fluorescent probe; analyzing and determining Fe < 3 + >: taking the diluent of the prepared CuCDs solution, adding the diluent into a centrifugal tube containing a Fe < 3 + > standard solution, uniformly mixing, carrying out fluorescent quantitative analysis and detection, and exploring the change rule of the fluorescence intensity ratio of CuCDs; according to the rapid detection method for Fe < 3 + > provided by the invention, the synthesized CuCDs have high sensitivity, can realize on-site rapid detection of Fe < 3 + > in a water medium, and shows a wide application prospect in the field of environmental monitoring.

The invention relates to the technical field of microfluidics, in particular to a microfluidic isothermal amplification method and system based on multichannel spectrum detection, and the method comprises the following steps: adding a sample and a reaction premixed solution into a microfluidic chip to form a reaction liquid; at least two fluorescent probes are added into the reaction premixed solution; carrying out isothermal amplification reaction on the reaction liquid, and collecting spectrum time sequence data in the reaction process; and performing spectral deconvolution on the spectral time sequence data to respectively obtain an independent amplification curve corresponding to each fluorescent probe, and performing quantitative analysis. Aiming at the problem that a reaction amplification quantitative system in the prior art depends on a high-precision quantitative PCR instrument and an optical assembly and is relatively high in cost, a spectral deconvolution mode is introduced to separate independent amplification curves of all fluorescent probes on a calculation level, so that an expensive PCR instrument is omitted, and the cost is reduced. And the requirements on color filtering and detection precision of the optical assembly are reduced, and the method can be applied to detection scenes with relatively wide wave bands.

The invention discloses a fluorescent probe as well as a preparation method and application thereof. The invention provides a series of fluorescent probes with novel structures, and the fluorescent probes take 2-(isoquinoline-1-yl) pyrrole boron difluoride as fluorophores, so that the fluorescence quantum yield of the probes is improved, the detection resolution of nitric oxide is increased, and the detection sensitivity is improved. The fluorescent probe disclosed by the invention has the characteristics of large Stokes displacement, high fluorescence quantum yield, good selectivity and short response time.

The invention provides a fluorescence probe signal calibration method and system for rapid detection of multiple residues in a complex matrix, and the method comprises the steps: collecting a fluorescence signal of a to-be-detected sample, and analyzing the original concentration, the fluorescence average lifetime and the fluorescence peak emission wavelength of the to-be-detected sample from the fluorescence signal; calculating a life deviation between the fluorescence average life and a preset reference life, and a wavelength deviation between the fluorescence peak emission wavelength and a preset reference wavelength; calculating to obtain a signal fidelity index based on the life deviation and the wavelength deviation; according to a preset threshold interval in which the signal fidelity index falls, a corresponding calibration strategy is determined, and the calibration strategy is one of non-calibration, calibration execution or invalid marking; and when the calibration strategy is to execute calibration, calculating a total calibration factor based on the life deviation and the wavelength deviation, calibrating the original concentration by using the total calibration factor, and outputting the calibrated concentration.

The present application relates to the technical field of biological sensing, and discloses a composite functional porous metal membrane and detection system for monitoring and identifying airborne microorganisms, and a method for monitoring and identifying airborne microorganisms. The monitoring and identification method comprises: applying an electric field to the composite functional porous metal membrane, causing the membrane to freely switch charge and capture airborne microorganisms to be tested; adding a fluorescent probe to the composite functional porous metal membrane, which binds with the microorganisms to be tested to obtain a fluorescent sample, and collecting imaging of light emitted after irradiation of excitation light, to achieve preliminary determination and addressing of the microorganisms to be tested; performing Raman spectroscopy scanning on individual bacteria, and comparing with a standard Raman spectrum library to achieve species identification of the individual bacteria. Relying on the "composite functional porous metal membrane," an integrated process of "selective enrichment-total bacteria monitoring-species identification" is realized; the present invention takes selective enrichment of the microorganisms to be tested as an entry point, lays the foundation for preliminary judgment accuracy and species identification accuracy, and improves the efficiency and precision of monitoring and identifying airborne microorganisms.

The invention relates to the technical field of fluorescent probes, and provides a fluorescent probe luminescence brightness quantification method and system for coupling excitation wavelength detuning. The fluorescence probe luminescence brightness quantification method for coupling excitation wavelength detuning comprises the following steps: constructing an initial structure of a probe molecule, and optimizing a geometric structure of a ground state of the probe molecule to obtain a stable configuration of the ground state of the probe molecule; based on the stable configuration of the ground state of the probe molecule, the excitation states are calculated, and the excitation energy of the lowest n excitation states of the probe molecule and the absorption transition dipole moments of the probe molecule in the X, Y and Z directions are obtained; based on the stable configuration of the ground state of the probe molecule, optimizing the geometric structure of the lowest excited state of the probe molecule to obtain the emission energy of the lowest excited state of the probe molecule and the emission transition dipole moments of the probe molecule in the X, Y and Z directions; and calculating a luminance index in combination with the energy parameter and the transition dipole moment parameter so as to select an optimal probe. Therefore, accurate and rapid prediction of the luminescence brightness of the probe is realized.

The invention belongs to the technical field of biological medicine, and particularly relates to a method for detecting extracellular vesicle protein and application thereof. According to the invention, the design is optimized on the basis of the first-generation SENSORS technology, and a second-generation BarFllare high-sensitivity detection technology is developed. According to the technology, a gold nanoparticle-mediated three-function fluorescent probe controllable release system is integrated, a superparamagnetic nanoparticle-mediated fluorescent probe captures enrichment and enhances a fluorescent signal through a spherical hot spot aggregation effect, the detection sensitivity is improved by 31 times compared with that of a traditional ELISA method, and high-sensitivity detection (1.37-7.30 parts / mu L) of EVs protein is achieved. Compared with the first-generation SENSORS technology, the BarFllare detection time is shortened by 43.75%, and the cost is reduced by about 63%.

The invention discloses an image processing method and system based on an in-situ hybridization technology and a medium, and relates to the technical field of biological information, and the method comprises the following steps: converting a cell DAPI dyeing result into two-dimensional data from three-dimensional data by utilizing Z-axis maximum intensity projection, identifying and separating a single cell from the two-dimensional data by utilizing cell segmentation, and when the cell segmentation is used for processing an overlapping region, identifying and separating the single cell from the two-dimensional data. Using a registration algorithm Ashlar to calculate an error between adjacent visual fields of the same round to obtain cell position information; an imaging result after hybridization of the fluorescent probe and the gene is subjected to multiple rounds of registration through a registration algorithm Ashlar to generate a spliced image, local maximum values of the spliced image under different rounds are marked by using a fluorescent dot recognition algorithm, so that position information of each fluorescent dot is obtained, the position information of each fluorescent dot corresponds to the gene in a transcript, and the position information of each fluorescent dot corresponds to the gene in the transcript. Obtaining gene position information; distributing genes into cells by utilizing the cell position information and the gene position information to obtain a cell gene matrix and displaying the cell gene matrix; according to the image processing method and system and the medium, accurate matching of sequencing data and spatial information is achieved, and then the distribution rule of gene expression in space is better revealed.

The invention belongs to the technical field of biomedical functional dyes and probes, and relates to a fluorescent probe, in particular to a cyclodextrin-based near-infrared fluorescent probe, which is a conjugate formed by coating a fluorescent molecule with cyclodextrin. According to the near-infrared fluorescent probe disclosed by the invention, cyclodextrin is nested on a carbon chain of a fluorescent molecule, so that the water solubility, histocompatibility and safety are improved, the fluorescence lifetime is prolonged, the light stability is improved, and rapid transmission and response of ureters, lymphatic vessels and lymph nodes in vivo are realized; the method can be used for NIR-I and NIR-II imaging; a near-infrared fluorescence probe with targeting property can be formed by marking various tumor targeting molecules, so that accurate targeting fluorescence imaging of different types of tumors such as urogenital tumors, head and neck cancers, breast cancers and cervical cancers and metastatic lymph nodes in NIR-I and NIR-II windows is realized, and the near-infrared fluorescence probe can be applied to fluorescence imaging navigation in clinical operations.

The invention discloses a preparation method and application of a dual-channel multifunctional multicolor fluorescent probe, and relates to the technical field of fluorescent sensing detection.The dual-channel multifunctional multicolor fluorescent probe is prepared through the following steps that pyromellitic acid and ZrCl4 solids are dispersed in deionized water, Zr-MOF is prepared through a hydrothermal method, pamoic acid and melamine are dispersed in deionized water, and the Zr-MOF is prepared through a hydrothermal method; the preparation method comprises the following steps: preparing green fluorescent carbon dots (CDs) by using a hydrothermal method, dispersing the CDs and Zr-MOF in deionized water according to a certain proportion, reacting, collecting a brown solid, washing, drying, dispersing the solid in ethanol, adjusting the pH to be alkaline, adding Eu (NO3) 3.6 H2O, reacting, filtering, and drying in vacuum to obtain a target probe. The probe can accurately distinguish Cu < 2 + > ions of tetracycline antibiotics (TCs) through two independent fluorescent channels, not only displays completely independent spectral response, but also has the advantages of self-calibration, high sensitivity, interference resistance, field visual detection and the like. The multifunctional fluorescent probe has important significance for improving detection efficiency, reducing cost and energy consumption, optimizing resource utilization and promoting green low-carbon economy.

This invention discloses a cellulose acetate-based 1,8-naphthalenedimide fluorescent probe for detecting hydrazine, its preparation method, and its application. The invention uses 4-bromo-1,8-naphthalenedimide as a raw material, which undergoes a condensation reaction with 4-aminobutyric acid to obtain 4-(4-bromo-1,8-naphthalenedimide)butyric acid (BNA-B); BNA-B undergoes a coupling reaction with 4-formylphenylboronic acid to obtain 4-(4-(4-formylphenyl)-1,8-naphthalenedimide)butyric acid (FNA-B); compound FNA-B then undergoes an esterification reaction with cellulose acetate (CA) to obtain the cellulose acetate-based 1,8-naphthalenedimide fluorescent probe FNA-B-CA. This compound specifically recognizes hydrazine. Under 365 nm ultraviolet light irradiation, the addition of hydrazine to a DMF / H2O solution of FNA-B-CA changes the fluorescence color of the solution from bright blue to colorless, indicating a detection limit of 8.4 × 10⁻⁶ for hydrazine. ‑8 M has promising applications as a fluorescent probe for the detection of hydrazine.

The invention relates to a fluorescent probe for hypochlorous acid detection and a preparation method and application thereof, the chemical name of the fluorescent probe for hypochlorous acid detection is 5-butyl-10, 10-difluoro-9-(4-(methylthio) phenyl)-5-hydro-4H, 10H-9 lambda 4, 10 lambda 4-benzo [de] [1, 3, 2] oxazaboraza [6, 5-g] isoquinoline-4, 6-diketone, and the structural formula of the fluorescent probe for hypochlorous acid detection is as shown in the following formula I. The invention further discloses a preparation method of the fluorescent probe for hypochlorous acid detection. According to the fluorescent probe for hypochlorous acid detection, N-butyl-3-aldehyde-4-hydroxy-1, 8-naphthalimide and (E)-2-butyl-6-hydroxy-5-(((4-(methylthio) phenyl) imino) methyl)-1H-benzo [de] isoquinoline-1, 3 (2H)-diketone are used as raw materials, and the fluorescent probe can be used for detecting hypochlorous acid. Compared with the prior art, the fluorescent probe for hypochlorous acid detection is simple and convenient in synthesis method, simple and easily available in raw materials, good in hypochlorous acid selectivity, relatively high in sensitivity and large in Stokes shift, and has a very good application prospect in hypochlorous acid detection.

The invention discloses a tetrahydrocarbazole enhanced fluorescent probe for detecting hydrosulfite as well as a preparation method and application of the tetrahydrocarbazole enhanced fluorescent probe. The fluorescent probe is a 2-(2-(5-(2, 2-dimethyl-2, 3, 4, 9-tetrahydro-1H-1, 3-methyl carbazole-6-yl) furan-2-yl) vinyl)-3-ethyl benzothiazole-3-onium iodised salt compound, namely, TC-FV-BT, and the fluorescent probe can be applied to the field of fluorescence detection. The probe TC-FV-BT can be used for specifically recognizing the hydrosulfite. Under sunlight, after bisulfite is added into a DMF / H2O (2: 8, v / v) solution of the probe TC-FV-BT, the color of the solution is changed from dark pink to white, under irradiation of ultraviolet light with the wavelength of 365 nm, the solution emits orange fluorescence at the emission wavelength of 605 nm, the fluorescence color of the solution is changed from colorless to orange, the response time is 4 min, the detection limit of the bisulfite reaches 77.27 nM, and the detection limit of the bisulfite reaches 77.27 nM. The fluorescent probe shows good selectivity and anti-interference performance, and has a good application prospect in the fields of environmental analysis, food and the like as a fluorescent probe for detecting hydrosulfite.

Provided is a temperature-responsive fluorescent particle comprising at least one type of fluorescent molecule in a molecular assembly comprising and constituted by at least one type of amphiphilic molecule, wherein the fluorescent molecule emits fluorescence when the molecular assembly is in the liquid phase and it is quenched when the molecular assembly is in the solid phase, due to a temperature-responsive solid-liquid phase transition, so that fluorescence emission and quenching of the fluorescent molecule are reversibly switched in a temperature responsive manner. Also provided is a temperature-responsive fluorescent probe comprising the temperature-responsive fluorescent particle the surface of which is modified with a biomolecule recognition element, and methods for detecting and quantitatively determining a biomolecule with the temperature-responsive fluorescent probe.

This invention discloses a near-infrared two-photon dual-mode linked response NTR&Cys fluorescent probe, its preparation method, and its application, belonging to the field of medical detection technology. The structural formula of the near-infrared two-photon dual-mode linked response NTR&Cys fluorescent probe is as follows: The near-infrared two-photon dual-mode linked response NTR&Cys fluorescent probe of this invention possesses a hypoxia dual-mode visual monitoring molecular framework, enabling dual-mode determination of hypoxia microenvironments. It improves the stability of the cyanine near-infrared framework and achieves highly stable and specific detection of nitroreductase under hypoxia. Under hypoxia conditions, the product reacts with NTR, and the single-photon excited near-infrared fluorescence is quenched and then illuminated. Simultaneously, under hypoxia, the probe reacts with Cys to generate a two-photon excited fluorescence signal, effectively avoiding the probe consumption problem of dual-response systems and ensuring the non-interference of fluorescence excitation and emission (dual-mode), thus truly achieving accurate locking of hypoxia microenvironments.

The invention discloses a fluorescent probe for rapidly detecting acetylene, the fluorescent probe is a metal nanocluster solution, and the metal nanocluster solution has a fluorescence emission peak and is used for being in contact with acetylene to generate static fluorescence quenching. The invention further discloses a preparation method, the problems that an existing gas chromatography technology for detecting acetylene is long in consumed time, high in operation specialty and high in cost are solved, and the glutathione modified copper nanocluster solution has high sensitivity and linear response, can detect acetylene in a low concentration range (such as 0.26 nL / L-26000 mu L / L) and has a good linear relation. The stability of the GSH-Cu NCs solution is good, the probe solution can be stored for a long time, and the fluorescence lifetime is stable. The glutathione modified copper nanocluster solution has strong anti-interference capability, and the GSH-Cu NCs solution has the advantages of high detection speed, simple and convenient acetylene detection operation, low cost and no need of complex instruments, and is suitable for field use.

The application relates to a quality inspection technology of inorganic salt, and discloses a detection reagent and a rapid detection method for high-purity lithium carbonate. A water-soluble fluorescent probe detection reagent is obtained by using 2-(4-chlorophenyl) benzoxazole, 2-(2-hydroxyphenyl) benzoxazole and other benzoxazole compounds as a substrate, and by modifying the substrate with glycerol, tris(hydroxyl) aminomethane and other compounds; high-purity lithium carbonate is detected by using the detection reagent; the high-purity lithium carbonate is solid-phase prepared into lithium carbonate suspension liquid; the upper liquid phase of the lithium carbonate suspension liquid is taken; the fluorescent probe detection reagent is added into the upper liquid phase; and the high-purity lithium carbonate is rapidly detected according to the fluorescent spectrum and a working curve. The application can realize the rapid detection of the high-purity lithium carbonate in a water-soluble environment, and the detection response time can be as short as 3-10 seconds.

The invention relates to the technical field of medical image processing, and discloses an intelligent counting method, system and device for fluorescence signals in a FISH image, and the method comprises the steps: extracting the multi-dimensional features of red and green fluorescence probes in a breast cancer FISH image through a full-automatic process; in combination with differential Gaussian enhancement algorithm preprocessing, multi-scale feature extraction and a neural network model of a self-attention mechanism, accurate counting of fluorescence signals is realized. According to the invention, a difference Gaussian enhancement algorithm is adopted to suppress background noise and highlight a fluorescent point area; traditional features are expanded, four dimensions of geometry, strength, shape and quality are covered, and fluorescent dot features are comprehensively described; capturing feature information of different levels by adopting a three-branch parallel feature extractor; through a space-channel double attention mechanism, the positions of important fluorescent points are automatically identified, and discriminative feature dimensions are intelligently selected; the optimal feature representation is automatically learned through the deep neural network, the weight is automatically adjusted according to the training stage, and the processing efficiency is improved.

The invention relates to the technical field of biomedical detection, and discloses a portable urine component detection system and method based on a fluorescence sensor array. The system comprises a fluorescence sensor array, different fluorescence probes are used for detecting different trace components in urine, and fluorescence color gradient changes are generated through a probe-target object reaction, so that bilirubin, glucose and albumin in the urine are detected. The detection system comprises a micro-fluidic chip which integrates a capillary driving channel and a colorimetric reaction area and is used for sample transmission and reaction area positioning; an ultraviolet excitation light source and a multi-angle optical sensor are arranged in the camera obscura type color acquisition module, and the camera obscura type color acquisition module eliminates ambient light interference and captures fluorescence chromaticity signals; and the intelligent data processing system is integrated with an intelligent analysis end of the smart phone, establishes a quantitative relationship between chromaticity parameters (RGB and hue saturation) and the concentration of a target object through a machine learning model, and outputs a detection result in combination with an ambient light correction algorithm.

The invention belongs to the technical field of targeting peptides, and particularly relates to an EGFR targeting peptide, a fluorescent probe, a composition and application. The targeting peptide has an amino acid sequence as shown in SEQ ID NO: 1. Through verification, the targeting peptide has the capability of selective enrichment in tumor cells, and is beneficial to tumor recognition and treatment. The fluorescent probe coupled by the targeting peptide and the ICG fluorescent dye can specifically recognize and target an EGFR receptor, realizes effective aggregation and retention at a tumor part, is suitable for targeted diagnosis and treatment of tumors, can realize accurate imaging, has important clinical transformation potential, and becomes a molecular imaging probe with application prospects.