The invention provides a liver cancer detection kit based on liquid biopsy. The liver cancer detection kit comprises staining enhancing liquid and specific antibodies with fluorescent staining labels, wherein the staining enhancing liquid is used for enhancing a staining effect; the specific antibodies include an ASGPR1 antibody, a CD45 antibody and a CPS1 antibody; and the staining enhancing liquid contains a surfactant with a concentration of 0.001mg / mL-1mg / mL. The kit provided by the invention can be used for effectively enriching target cells and determining whether the enriched target cells are derived from early-phase liver cancer patients. Meanwhile, by virtue of double-tumor marker detection, the tumor detection sensitivity is increased, and furthermore, the detection accuracy is guaranteed by virtue of CEP8 detection. Besides, by utilizing the staining enhancing liquid, the staining effect is enhanced, multiple antibodies with the fluorescent staining labels can be integrated with the target cells, and the target cells are stained, so that the staining effect is relatively good, the fluorescence is relatively strong, and borders are clear.