Gene group and kit for diagnosing lung caner, and diagnosis method thereof

Abstract

The invention relates to the field of genetic engineering and biotechnologic detection, and concretely relates to a gene group and a kit for diagnosing lung cancer, and a diagnosis method thereof. The gene group for diagnosing the lung cancer includes ABCB1, AKT1, ALK, APC, ATIC and other 63 genes. The method using the gene group to diagnose the lung cancer comprises the following steps: (1) extracting free DNA and genome DNA from the plasma of a sample to be detected; and (2) breaking the genome DNA into fragments with the length of 150-250 bp, carrying out hybrid capture on the broken genome DNA and the free DNA to construct a DNA library, carrying out online sequencing, and analyzing the obtained sequencing result. Exon and partial intron regions of the 68 genes of the free DNA are enriched at one time by a probe capture technology to realize multi-gene and multi-target parallel deep high-throughput sequencing with high accuracy, optimize the detection flow and improve the detection precision, so liquid biopsy, low-frequency detection and tumor diagnosis become possible.

Description

technical field [0001] The invention relates to the fields of genetic engineering and biotechnology detection, in particular to a gene group, a kit and a diagnostic method for lung cancer diagnosis. Background technique [0002] Circulating DNA is a kind of extracellular DNA in a cell-free state, which exists in body fluids such as blood, synovial fluid, and cerebrospinal fluid. It is mainly composed of single-stranded or double-stranded DNA and a mixture of single-stranded and double-stranded DNA. Complex or free DNA exists in two forms. As a new tumor marker, circulating DNA will play an important role in the diagnosis, treatment and prognosis of tumors, especially for some tumors that do not have typical clinical symptoms, non-specific examination and difficult diagnosis can avoid complex, Invasive biopsy. [0003] Circulating tumor DNA (ctDNA) refers to the somatic DNA of tumor cells that is shed or released into the circulatory system after apoptosis. With the rapid d...

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Problems solved by technology

[0005] The problem of the prior art that the present invention solves is: for existing lung cancer detection method usually has the following defect and deficiency: first, traditional detection method, comprises surgical operation treatment, radiotherapy and chemotherapy etc., can't detect tumor early Related symptoms; second, the existing detection methods based on lung cancer genes usually require a relatively high initial amount of library construction, and the sequencing sensitivity and accuracy are relatively low; third, the existing libraries for tumor gene detection, The genes covered are either too many or too few, too many will increase the burden of lung cancer detection; too few, the capture of tumor-related genes is very small, and many important gene mutation information will be missed; or the existing detection methods , covering some genes that are not very related to tumors, and cannot fully and effectively reflect the mutations of lung cancer and lung cancer-related genes

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