56 results about "Synovial fluid" patented technology

The invention provides a microfluidic chip, system and method for osteochondral organs, and relates to the technical field of organoid microfluidic chips, the chip comprises a bone microvascular endothelial cell channel, a bone compartment, a cartilage compartment and a synovial fluid channel which are arranged in parallel and in sequence, and permeation areas between the channel and the compartments and between the compartments. According to the scheme, the complex structure and function of a human osteochondral system are effectively simulated, the physiological and pathological processes of human bone joints can be simulated more truly in an in-vitro environment, and a method for establishing a highly-simulated femoral head necrosis model and osteoarthritis model based on the chip is provided. The problems that a traditional model is insufficient in physiological environment simulation, lack of control over dynamic culture conditions, tedious and time-consuming in the construction process, difficult to support high-throughput experiments and the like are solved.

The invention relates to the technical field of online detection and analysis, and discloses a bone joint synovial fluid detection and analysis system based on microfluidics, which specifically comprises the following steps: acquiring synovial fluid rheological data and synovial fluid molecular activity data in a bone joint movement process and cartilage surface morphological data in a joint buckling process through a biosensor; constructing a synovial fluid dynamic anomaly analysis model, and performing synovial fluid function anomaly analysis through the synovial fluid rheological data and the molecular activity data; a cartilage stress anomaly analysis model is constructed, and cartilage stress anomaly analysis is carried out through cartilage surface morphological change data; obtaining a synovial fluid function abnormality analysis result and a cartilage stress abnormality analysis result to carry out bone joint function adaptability analysis; and acquiring a bone joint function adaptability analysis result and carrying out synovial fluid function abnormality analysis early warning. The problem that in the prior art, due to the fact that the bionic degree of a detection strategy is insufficient and bone joint dynamic parameters are deficient, the association degree of a static synovial fluid detection result and the actual joint rehabilitation condition of a patient is poor is solved.

The invention belongs to the technical field of biological medicines, and particularly discloses application of ORM1 protein or VIM knockout adenovirus in preparation of a medicine for treating temporomandibular joint osteoarthritis (TMJOA). The invention proves that the expression of ORM1 is reduced in synovial fluid of a TMJOA patient and a unilateral anterior tooth reverse inhale model rat, and the degradation of cartilage matrix of the UAC rat can be remarkably inhibited and the progress of TMJOA can be relieved by locally injecting ORM1 protein. On the mechanism level, ORM1 is combined with waveform protein VIM to inhibit an MAPK channel, so that expression of MMP13 and MMP3 is reduced, cartilage matrix degradation is reduced, and cartilage steady state is maintained. In addition, the invention also finds that after the VIM of the rat is knocked out by using the adeno-associated virus, the progress of TMJOA, including cartilage degeneration and subchondral bone destruction, is also obviously improved. Therefore, the invention provides a new therapeutic target and a new therapeutic strategy for the TMJOA.

The invention belongs to the field of pet food processing, and relates to a premix for improving joint health of pets and a preparation method thereof. The premix is added with the following natural components: rhizoma polygonati, rhizoma drynariae, eucommia ulmoides, ligusticum wallichii, liquorice, turmeric, poria cocos and Chinese yam. The whole formula has the effects of tonifying liver and kidney to consolidate the root, promoting blood circulation to remove meridian obstruction to treat symptoms, tonifying spleen to remove dampness to assist transportation and transformation, tonifying spleen to prevent dampness stagnation, promoting diuresis to prevent positive qi injury, treating both symptoms and root causes, combining dredging and tonifying, and directly reaching the effects on joints. The premix can tonify cartilage, strengthen ligament, relieve pain, supplement synovial fluid, effectively improve the arthritis health of pets and improve the life quality of pets.

A temporomandibular joint replacement system includes an insert configured to be disposed on an articular surface of a temporomandibular joint, a biocompatible fluid including a synthetic synovial fluid, a porous condyle configured to contain the biocompatible fluid and to be disposed on an opposing surface of the temporomandibular joint, and a sensor disposed along a top surface of the insert.

The invention relates to the technical field of biological medicine, in particular to a biomarker for diagnosing and distinguishing rheumatoid arthritis and knee osteoarthritis and application of the biomarker. In order to solve the problems of insufficient sensitivity and specificity of rheumatoid arthritis (RA) and knee osteoarthritis (KOA) in clinical detection, the invention discloses a novel biomarker, namely a soluble interleukin 17 receptor D, namely sIL-17RD. The inventor finds that the sIL-17RD level is remarkably increased in serum of a patient with rheumatoid arthritis, the sIL-17RD level is not remarkably changed in serum of a patient with knee osteoarthritis, and the sIL-17RD level is remarkably increased in synovial fluid of the patient with knee osteoarthritis. Therefore, by measuring the sIL-17RD level in the serum sample of the rheumatoid arthritis patient and the sIL-17RD level in the synovial fluid and serum of the knee osteoarthritis patient, the rheumatoid arthritis and the knee osteoarthritis are diagnosed and distinguished, and a new strategy is provided for clinical diagnosis and distinguishing of the rheumatoid arthritis and the knee osteoarthritis.

The present invention relates to pharmacogenetics, more specifically to strategies involving biomarkers associated with the clinical response to a compound before or during treatment of a cartilage disorder, such as osteoarthritis. The present invention more particularly relates to the combination of JSW measurements and level of specific proteins present in the blood, serum, synovial fluid or in the urine, which can be used in strategies such as patients' enrichment in clinical trials, patients' selection strategy before or during treatment or for adapting the treatment of a patient in the frame of treatments for cartilage disorder, such as osteoarthritis.

The invention relates to the technical field of nano medicines, in particular to a tanshinol-containing copper-based metal organic framework nano material and a preparation method and application thereof.According to the prepared tanshinol-containing copper-based metal organic framework nano material, tanshinol is dissolved in a dimethylimidazole aqueous solution, copper ions are rapidly added into the solution, and the tanshinol-containing copper-based metal organic framework nano material is obtained after the tanshinol is dissolved in the dimethylimidazole aqueous solution; the tanshinol-loaded copper-based metal organic framework nano-material is prepared from the copper-based metal organic framework nano-material, the tanshinol-loaded copper-based metal organic framework nano-material has high drug loading rate and acid-sensitive drug release performance, and the defects that a small-molecule drug tanshinol is poor in stability in an articular cavity and is easily removed by synovial fluid can be overcome. Compared with a natural enzyme, the nano-enzyme formed by combining tanshinol and copper-based MOF has the active oxygen scavenging capacity, and the treatment effect on osteoarthritis is jointly improved.

The present invention relates to a hydrogel composition for intra-articular administration, which is easily injected into the human body, and use thereof for supplementing joint synovial fluid, supplementing viscosity, and preventing or treating intra-articular lesions. The hydrogel composition according to the present invention can improve the gelation temperature, viscosity, biodegradability, and tissue support capability of hyaluronic acid and, at the same time, can improve practitioner convenience and patient compliance due to the reduced injection pressure thereof. In addition, the hydrogel composition can increase the effect of ameliorating intra-articular lesions when co-administered with stem cells or exosomes, and thus can have various applications in medical devices and pharmaceutical fields for the purpose of cartilage regeneration and intra-articular lesion treatment.

Disclosed are methods for detecting extracapsular synovial fluid or components thereof as a marker for initiation of processes that lead to degenerative joint disease in a subject.

The present invention discloses an application and a kit for differential histoproteins and / or metabolites of pigmented villonodular synovitis; the differential histoproteins include at least one of the following proteins: TNFSF11, cathepsin K, and adhesion G protein-coupled receptor E5; the differential metabolites include at least one of the following products: 13-hydroxyperoxylinoleic acid and octadecadienoic acid; the present invention provides a new screening marker for pigmented villonodular synovitis (PVNS) through proteomics and metabolomics, which can achieve effective screening for pigmented villonodular synovitis; the obtained screening marker is used in a screening kit, which only needs synovial fluid as a test sample, can quickly perform screening and detection, and has the advantages of high efficiency and simple detection.

The present invention relates to biomarkers associated with the clinical response to an FGF-18 compound before or during treatment of a cartilage disorder. The present invention more particularly relates to specific proteins present in the blood, serum, synovial fluid or in the urine, which can be used for the diagnosis and treatment of cartilage disorders. The invention further discloses specific proteins that are related to cartilage response to an FGF-18 compound treatment as well as diagnostic tools and kits based on their expression profile. Thus, the invention can be used in predicting the response to an FGF-18 compound treatment, before starting the treatment with FGF-18 or during the treatment. It could be used for selecting / identifying subjects to be treated by intra-articular administration of an FGF-18 compound. The use of these biomarkers in diagnostics could result in increased benefit and reduced risk in subjects.

To provide a method for restoring the synovial surface properties of a synovial surface film that has been damaged and whose synovial surface properties have deteriorated. [Solution] A method for restoring the synovial surface properties of a synovial surface film, comprising a standing step of allowing the damaged synovial surface film, whose synovial surface properties have deteriorated, to stand for more than 5 minutes, wherein the synovial surface film is a synovial surface film containing a liquid organic compound at 25°C and 1 atm.

The invention relates to the technical field of biomedical materials, in particular to double-responsiveness hydrogel as well as a preparation method and application thereof. The invention provides a dual-responsiveness injectable hydrogel. The dual-responsiveness injectable hydrogel comprises oxidized hyaluronic acid (OHA) and hyaluronic acid-3-aminophenylboronic acid (HA-PBA), the OHA and the HA-PBA are connected through a Schiff base bond; hA and PBA in the HA-PBA are connected through a boric acid ester bond. The hydrogel has ROS (reactive oxygen species) and pH (potential of hydrogen) double responsiveness due to existence of borate bonds and Schiff base bonds, and is suitable for being used as a carrier of a rheumatoid arthritis drug delivery system; hyaluronic acid is a main component of synovial fluid and is beneficial to relieving pain of a patient suffering from rheumatoid arthritis; the hydrogel is simple in used raw materials and simple and convenient in preparation process, and is expected to be applied to local drug delivery of rheumatoid arthritis and other pathological microenvironment diseases with high active oxygen or low pH.

The invention provides methods and systems for detecting a biomarker in a synovial fluid wherein the system also includes a control to ensure that the test sample is indeed synovial fluid. The biomarkers and the control for synovial fluid can be identified using proteomic methods, including but not limited to antibody based methods, such as an enzyme-linked immunosorbant assay (ELISA), a radioimmunoassay (RIA), or a lateral flow immunoassay.

The present invention relates to the technical field of Chinese veterinary drug preparations, and proposes a solid-liquid mixed veterinary drug preparation and a preparation method thereof, wherein the raw materials of the veterinary drug preparation include plant-derived Chinese medicinal materials, cellulase, acid protease, and pectinase. Through the above technical scheme, the main components of the plant-derived Chinese medicinal materials are extracted, and the original medicinal materials are retained. The prepared solid-liquid mixed preparation is easier to absorb, and the problem that the preparation process of the Chinese veterinary drug preparation in the prior art is complicated and the effect on the prevention and treatment of chicken synoviae Mycoplasma is poor is solved.

The present application relates to the technical field of genetic testing, and discloses a sample diluent, a sample preparation method, and a rheumatoid arthritis test kit. The sample diluent comprises the following concentrations of components: 5-40 g / L of an alkali metal salt, 1-20 g / L of a protein protectant, 5-50 mM of an alkyl glycoside surfactant, 0.01-0.1% of a Tween surfactant, 1-100 μg / mL of an immunoadsorbent, and 10-200 mM of a buffer, wherein the pH value of the buffer ranges from 6.5 to 8.0. The present application provides an actual basis for synovial fluid testing in clinical rheumatoid arthritis, and takes into account a small sample size, such as testing of a site where synovial fluid is difficult to aspirate. In addition, by using an iFlash series instrument, a result can be obtained in as little as 10 min. Compared with the operating time of at least 1 hour of conventional ELISA, the test process is greatly accelerated, and high sensitivity and specificity are achieved.

The invention provides an injectable magnesium-loaded lipid-based super-lubricating hydrogel microsphere, an articular cavity injection preparation and a preparation method of the injectable magnesium-loaded lipid-based super-lubricating hydrogel microsphere. The method comprises the following steps: S1, preparing a magnesium-loaded multilayer vesicular liposome suspension containing magnesium ions; s2, preparing a non-crosslinked magnesium-containing hydrogel; and S3, mixing the magnesium-loaded multilayer vesicular liposome suspension and the magnesium-containing hydrogel to prepare hydrogel liquid drops, and carrying out crosslinking to form the magnesium-loaded lipid-based super-lubricating hydrogel microspheres. The magnesium loading amount of the lipid-based super-lubrication hydrogel microspheres can be greatly improved; on one hand, the biological function provided by magnesium ions can be greatly prolonged, that is, repairing of cartilage by synovial fluid progenitor cells is continuously promoted; on the other hand, the self-lubricating effect of the lipid-based super-lubricating hydrogel microspheres can reduce the secondary wear of the new cartilage; in addition, magnesium ions can remarkably promote expression of cartilage cells PRG4, so that the stable state of the cartilage cells can be maintained, and joint lubrication can be further enhanced.

The invention relates to the technical field of medical image navigation, in particular to a gout minimally invasive treatment multi-modal image navigation system which comprises a data acquisition module, a biomechanical fusion modeling module, a dynamic path planning module and a multi-physical field execution control module. The data acquisition module realizes synchronous acquisition of crystal-structure stress gradient, surface energy density and viscoelasticity parameters of the synovial fluid; the fusion modeling module generates a three-dimensional crystallization adhesion risk coefficient and fluid resistance prediction matrix based on a finite element and fluid-solid coupling algorithm; the path planning module dynamically calculates motion compensation and suction parameters in combination with real-time instrument poses, and outputs a six-degree-of-freedom navigation instruction set; and the execution control module drives the magnetic navigation puncture needle, the self-adaptive suction device and the laser ablation unit to realize cooperative execution of pose regulation and control, flow regulation and focus form switching. According to the method, gout crystallization accurate identification, path risk-avoiding navigation and targeted removal are realized, and the safety and the removal rate of minimally invasive intervention are remarkably improved.

This invention provides an exercise assistance device that assists the user in moving their hip joint in multiple directions, thereby improving the effects of increased synovial fluid, stretching, and muscle training. [Solution] The exercise assist device comprises a base that is placed on the floor, a seat on which the user sits, and legs connecting the base and the seat, the legs having at least one of a first connecting portion that rotatably connects the lower end to the base and a second connecting portion that rotatably connects the upper end to the seat.

A medical device includes a needle, a syringe, and a probe assembly respectively connected to a main body. The syringe may include a cavity in fluid communication with a bore through a shaft of the needle and is configured to draw a fluid into the cavity through the needle. The probe assembly may include a probe that is movable through the bore.

The invention discloses a method for preparing a Pickering emulsion bionic synovial fluid of Chinese herbal volatile oil, and relates to the technical field of biolubricants. The method comprises: adding nanoparticles to ultrapure water, and ultrasonically dispersing to obtain a nanoparticle dispersion; adding Chinese herbal volatile oil to the nanoparticle dispersion according to a certain volume fraction, and ultrasonically stirring to obtain a Pickering emulsion bionic synovial fluid of Chinese herbal volatile oil. The Pickering emulsion bionic synovial fluid of Chinese herbal volatile oil prepared by the invention has excellent friction reduction and anti-wear properties at the interface of artificial joints and articular cartilage, and can effectively remove active oxygen free radicals in the environmental system, and can inhibit and alleviate the onset of osteoarthritis from the two aspects of controlling mechanical stress and reducing oxidative stress.

The invention provides a biochip for detecting a marker LTF in synovial fluid as well as a preparation method and application of the biochip. The biochip comprises a solid phase carrier and an immunodetection system, the solid-phase carrier comprises a plastic substrate and a water-soluble glue layer; the immunodetection system comprises a photonic crystal array formed by self-assembly on a plastic substrate, and a biological material chemically modified on the surface of the photonic crystal array, the biological material is used for targeted recognition of a target detection object; the biological material is selected from lactoferrin capture antibodies; the target detection object is lactoferrin. The biochip disclosed by the invention has the advantage of rapid detection, and the reaction time of the detection method disclosed by the invention can be shortened to 10 minutes as different from that of an ELISA (enzyme-linked immuno sorbent assay) method which needs 4 hours, so that the detection efficiency and the practical application value of the biochip are greatly improved.

The invention discloses a detection kit for detecting the content of calprotectin in synovial fluid. The detection kit comprises a detection kit shell and a calprotectin immunochromatography test strip, and the calprotectin immunochromatography test strip is arranged in the detection kit shell; the calprotectin immunochromatography test strip comprises a sample pad, a glass fiber combination pad, a coated nitrocellulose membrane and a water absorption pad which are bonded in sequence, an anti-calprotectin antibody marked by colloidal gold is embedded in the glass fiber combination pad and is used for being combined with calprotectin in a sample to be detected to form a compound; the anti-calprotectin monoclonal antibody is coated on a detection line of the nitrocellulose membrane, and the anti-mouse IgG antibody is coated on a quality control line of the nitrocellulose membrane; the colloidal gold labeled anti-calprotectin antibody is embedded in the glass fiber cushion layer, and the image recognition module is used for collecting a tested test paper image and calculating a relative gray value of a detection line and a quality control line, so that a calprotectin concentration interval is judged.

The invention provides a collagen composite material for joint injury repair and a preparation method and application thereof.The collagen composite material comprises collagen and chitosan, the chitosan is water-soluble chitosan subjected to one or two or more combined treatment of carboxylation, hydroxylation, phosphatization and quaternization, and the chitosan is water-soluble chitosan subjected to one or two or more combined treatment of carboxylation, hydroxylation, phosphatization and quaternization. The weight ratio of the collagen to the water-soluble chitosan is (1: 15)-(1: 3). The composition and proportion of the collagen and the water-soluble chitosan are scientifically screened, the collagen composite material which is closer to natural synovial fluid in composition is formed, the water-soluble chitosan provides physical blocking inflammatory factors and a lubricating effect between cartilages, and the collagen has a pore structure, so that the cartilages have a good anti-inflammatory effect on the cartilages, and the cartilages have a good anti-inflammatory effect on the cartilages. Adhesion of cartilage cells is facilitated, nutrition is obtained, proliferation of the cartilage cells is promoted, and repair of damaged cartilage is achieved.

To provide pharmacogenetics, more specifically to strategies involving biomarkers associated with a clinical response to a compound before or during treatment of a cartilage disorder such as osteoarthritis.SOLUTION: The present invention more particularly relates to a combination of JSW measurements and a level of a specific protein present in the blood, serum, synovial fluid or urine, which can be used for adapting the treatment of a patient, in strategies such as patients' enrichment in clinical trials, patients' selection strategy before or during treatment or in a framework of treatments for cartilage disorder such as osteoarthritis.SELECTED DRAWING: Figure 1

The invention discloses a butyl-glycine cross-linked sodium hyaluronate gel and a composition and a preparation method thereof. The cross-linked sodium hyaluronate gel is a gel mixture formed by swelling gel of cross-linked hyaluronic acid and salt thereof in a sodium chloride phosphate physiological buffer solution, wherein the gel of the cross-linked hyaluronic acid and the salt thereof is prepared by cross-linking the hyaluronic acid or the salt thereof with a cross-linking agent; wherein the D50 particle size distribution range of the gel mixture is 300-3000 [mu] m, so that the gel mixture can better simulate the physical characteristics of healthy joint synovial fluid and provide more effective lubricating and buffering effects under the condition of the same gel molecular weight and energy storage elastic modulus, thereby relieving joint friction and pain and improving the curative effect of cross-linked sodium hyaluronate maintained in vivo.

The invention belongs to the technical field of biological medicine, and relates to application of ABT-737 in preparation of anti-inflammatory drugs, in particular to application of ABT-737 in preparation of drugs for treating refractory rheumatoid arthritis. It is found for the first time that ABT-737 can induce apoptosis of inflammatory T cells derived from peripheral blood or synovial fluid of a patient suffering from the refractory rheumatoid arthritis, reduce the number of the inflammatory T cells and further inhibit occurrence and development of inflammation, and therefore ABT-737 has the prospect of being developed into drugs for treating the refractory rheumatoid arthritis and anti-inflammatory or immunomodulatory drugs.

The invention belongs to the technical field of biological medicines, and particularly relates to application of Venetoclax in preparation of an anti-inflammatory drug, in particular to a drug for treating rheumatoid arthritis (RA) with insufficient (MTX-IR) by using methotrexate. It is found for the first time that Venetoclax can induce apoptosis of inflammatory T cells of peripheral blood or synovial fluid of rheumatoid arthritis patients with insufficient curative effect on methotrexate, and occurrence and development of inflammation are inhibited by reducing the number of the inflammatory T cells, so that Venetoclax has the prospect of being developed into drugs for treating rheumatoid arthritis and anti-inflammatory and immunomodulatory drugs.

The invention discloses lower limb strength testing equipment for old people, and belongs to the technical field of lower limb strength testing, the lower limb strength testing equipment comprises a sliding table, a detection mechanism and a rehabilitation training mechanism, the sliding table is mounted on a bed body, the detection mechanism is mounted on the sliding table, the detection mechanism comprises a fixed seat and a movable seat, and first pressure sensors are arranged on the two sides of the fixed seat respectively; the movable seat is mounted between the two first pressure sensors; the rehabilitation training mechanism comprises a supporting rod, a first driving assembly and a second driving assembly, and the supporting rod is provided with a pedal and a second pressure sensor. A user only needs to lie on the bed body to test the lower limb strength, so that the use convenience is improved, and the use safety is ensured; and through the rehabilitation training mechanism, secretion of synovial fluid of the joints of the user can be promoted, the flexibility of the joints can be improved, the muscle strength can be enhanced and recovered, the use requirements of the user for rehabilitation training are met, the use range is widened, the use functions are enriched, and good economical efficiency is achieved.