32 results about "Synovial fluid" patented technology

The invention relates to the technical field of online detection and analysis, and discloses a bone joint synovial fluid detection and analysis system based on microfluidics, which specifically comprises the following steps: acquiring synovial fluid rheological data and synovial fluid molecular activity data in a bone joint movement process and cartilage surface morphological data in a joint buckling process through a biosensor; constructing a synovial fluid dynamic anomaly analysis model, and performing synovial fluid function anomaly analysis through the synovial fluid rheological data and the molecular activity data; a cartilage stress anomaly analysis model is constructed, and cartilage stress anomaly analysis is carried out through cartilage surface morphological change data; obtaining a synovial fluid function abnormality analysis result and a cartilage stress abnormality analysis result to carry out bone joint function adaptability analysis; and acquiring a bone joint function adaptability analysis result and carrying out synovial fluid function abnormality analysis early warning. The problem that in the prior art, due to the fact that the bionic degree of a detection strategy is insufficient and bone joint dynamic parameters are deficient, the association degree of a static synovial fluid detection result and the actual joint rehabilitation condition of a patient is poor is solved.

The invention belongs to the field of pet food processing, and relates to a premix for improving joint health of pets and a preparation method thereof. The premix is added with the following natural components: rhizoma polygonati, rhizoma drynariae, eucommia ulmoides, ligusticum wallichii, liquorice, turmeric, poria cocos and Chinese yam. The whole formula has the effects of tonifying liver and kidney to consolidate the root, promoting blood circulation to remove meridian obstruction to treat symptoms, tonifying spleen to remove dampness to assist transportation and transformation, tonifying spleen to prevent dampness stagnation, promoting diuresis to prevent positive qi injury, treating both symptoms and root causes, combining dredging and tonifying, and directly reaching the effects on joints. The premix can tonify cartilage, strengthen ligament, relieve pain, supplement synovial fluid, effectively improve the arthritis health of pets and improve the life quality of pets.

The present invention relates to a hydrogel composition for intra-articular administration, which is easily injected into the human body, and use thereof for supplementing joint synovial fluid, supplementing viscosity, and preventing or treating intra-articular lesions. The hydrogel composition according to the present invention can improve the gelation temperature, viscosity, biodegradability, and tissue support capability of hyaluronic acid and, at the same time, can improve practitioner convenience and patient compliance due to the reduced injection pressure thereof. In addition, the hydrogel composition can increase the effect of ameliorating intra-articular lesions when co-administered with stem cells or exosomes, and thus can have various applications in medical devices and pharmaceutical fields for the purpose of cartilage regeneration and intra-articular lesion treatment.

Disclosed are methods for detecting extracapsular synovial fluid or components thereof as a marker for initiation of processes that lead to degenerative joint disease in a subject.

To provide a method for restoring the synovial surface properties of a synovial surface film that has been damaged and whose synovial surface properties have deteriorated. [Solution] A method for restoring the synovial surface properties of a synovial surface film, comprising a standing step of allowing the damaged synovial surface film, whose synovial surface properties have deteriorated, to stand for more than 5 minutes, wherein the synovial surface film is a synovial surface film containing a liquid organic compound at 25°C and 1 atm.

The invention relates to the technical field of biomedical materials, in particular to double-responsiveness hydrogel as well as a preparation method and application thereof. The invention provides a dual-responsiveness injectable hydrogel. The dual-responsiveness injectable hydrogel comprises oxidized hyaluronic acid (OHA) and hyaluronic acid-3-aminophenylboronic acid (HA-PBA), the OHA and the HA-PBA are connected through a Schiff base bond; hA and PBA in the HA-PBA are connected through a boric acid ester bond. The hydrogel has ROS (reactive oxygen species) and pH (potential of hydrogen) double responsiveness due to existence of borate bonds and Schiff base bonds, and is suitable for being used as a carrier of a rheumatoid arthritis drug delivery system; hyaluronic acid is a main component of synovial fluid and is beneficial to relieving pain of a patient suffering from rheumatoid arthritis; the hydrogel is simple in used raw materials and simple and convenient in preparation process, and is expected to be applied to local drug delivery of rheumatoid arthritis and other pathological microenvironment diseases with high active oxygen or low pH.

The present application relates to the technical field of genetic testing, and discloses a sample diluent, a sample preparation method, and a rheumatoid arthritis test kit. The sample diluent comprises the following concentrations of components: 5-40 g / L of an alkali metal salt, 1-20 g / L of a protein protectant, 5-50 mM of an alkyl glycoside surfactant, 0.01-0.1% of a Tween surfactant, 1-100 μg / mL of an immunoadsorbent, and 10-200 mM of a buffer, wherein the pH value of the buffer ranges from 6.5 to 8.0. The present application provides an actual basis for synovial fluid testing in clinical rheumatoid arthritis, and takes into account a small sample size, such as testing of a site where synovial fluid is difficult to aspirate. In addition, by using an iFlash series instrument, a result can be obtained in as little as 10 min. Compared with the operating time of at least 1 hour of conventional ELISA, the test process is greatly accelerated, and high sensitivity and specificity are achieved.

The invention provides an injectable magnesium-loaded lipid-based super-lubricating hydrogel microsphere, an articular cavity injection preparation and a preparation method of the injectable magnesium-loaded lipid-based super-lubricating hydrogel microsphere. The method comprises the following steps: S1, preparing a magnesium-loaded multilayer vesicular liposome suspension containing magnesium ions; s2, preparing a non-crosslinked magnesium-containing hydrogel; and S3, mixing the magnesium-loaded multilayer vesicular liposome suspension and the magnesium-containing hydrogel to prepare hydrogel liquid drops, and carrying out crosslinking to form the magnesium-loaded lipid-based super-lubricating hydrogel microspheres. The magnesium loading amount of the lipid-based super-lubrication hydrogel microspheres can be greatly improved; on one hand, the biological function provided by magnesium ions can be greatly prolonged, that is, repairing of cartilage by synovial fluid progenitor cells is continuously promoted; on the other hand, the self-lubricating effect of the lipid-based super-lubricating hydrogel microspheres can reduce the secondary wear of the new cartilage; in addition, magnesium ions can remarkably promote expression of cartilage cells PRG4, so that the stable state of the cartilage cells can be maintained, and joint lubrication can be further enhanced.

The invention relates to the technical field of medical image navigation, in particular to a gout minimally invasive treatment multi-modal image navigation system which comprises a data acquisition module, a biomechanical fusion modeling module, a dynamic path planning module and a multi-physical field execution control module. The data acquisition module realizes synchronous acquisition of crystal-structure stress gradient, surface energy density and viscoelasticity parameters of the synovial fluid; the fusion modeling module generates a three-dimensional crystallization adhesion risk coefficient and fluid resistance prediction matrix based on a finite element and fluid-solid coupling algorithm; the path planning module dynamically calculates motion compensation and suction parameters in combination with real-time instrument poses, and outputs a six-degree-of-freedom navigation instruction set; and the execution control module drives the magnetic navigation puncture needle, the self-adaptive suction device and the laser ablation unit to realize cooperative execution of pose regulation and control, flow regulation and focus form switching. According to the method, gout crystallization accurate identification, path risk-avoiding navigation and targeted removal are realized, and the safety and the removal rate of minimally invasive intervention are remarkably improved.

This invention provides an exercise assistance device that assists the user in moving their hip joint in multiple directions, thereby improving the effects of increased synovial fluid, stretching, and muscle training. [Solution] The exercise assist device comprises a base that is placed on the floor, a seat on which the user sits, and legs connecting the base and the seat, the legs having at least one of a first connecting portion that rotatably connects the lower end to the base and a second connecting portion that rotatably connects the upper end to the seat.

The invention provides a biochip for detecting a marker LTF in synovial fluid as well as a preparation method and application of the biochip. The biochip comprises a solid phase carrier and an immunodetection system, the solid-phase carrier comprises a plastic substrate and a water-soluble glue layer; the immunodetection system comprises a photonic crystal array formed by self-assembly on a plastic substrate, and a biological material chemically modified on the surface of the photonic crystal array, the biological material is used for targeted recognition of a target detection object; the biological material is selected from lactoferrin capture antibodies; the target detection object is lactoferrin. The biochip disclosed by the invention has the advantage of rapid detection, and the reaction time of the detection method disclosed by the invention can be shortened to 10 minutes as different from that of an ELISA (enzyme-linked immuno sorbent assay) method which needs 4 hours, so that the detection efficiency and the practical application value of the biochip are greatly improved.

The invention discloses a detection kit for detecting the content of calprotectin in synovial fluid. The detection kit comprises a detection kit shell and a calprotectin immunochromatography test strip, and the calprotectin immunochromatography test strip is arranged in the detection kit shell; the calprotectin immunochromatography test strip comprises a sample pad, a glass fiber combination pad, a coated nitrocellulose membrane and a water absorption pad which are bonded in sequence, an anti-calprotectin antibody marked by colloidal gold is embedded in the glass fiber combination pad and is used for being combined with calprotectin in a sample to be detected to form a compound; the anti-calprotectin monoclonal antibody is coated on a detection line of the nitrocellulose membrane, and the anti-mouse IgG antibody is coated on a quality control line of the nitrocellulose membrane; the colloidal gold labeled anti-calprotectin antibody is embedded in the glass fiber cushion layer, and the image recognition module is used for collecting a tested test paper image and calculating a relative gray value of a detection line and a quality control line, so that a calprotectin concentration interval is judged.

The invention discloses a butyl-glycine cross-linked sodium hyaluronate gel and a composition and a preparation method thereof. The cross-linked sodium hyaluronate gel is a gel mixture formed by swelling gel of cross-linked hyaluronic acid and salt thereof in a sodium chloride phosphate physiological buffer solution, wherein the gel of the cross-linked hyaluronic acid and the salt thereof is prepared by cross-linking the hyaluronic acid or the salt thereof with a cross-linking agent; wherein the D50 particle size distribution range of the gel mixture is 300-3000 [mu] m, so that the gel mixture can better simulate the physical characteristics of healthy joint synovial fluid and provide more effective lubricating and buffering effects under the condition of the same gel molecular weight and energy storage elastic modulus, thereby relieving joint friction and pain and improving the curative effect of cross-linked sodium hyaluronate maintained in vivo.

The invention belongs to the technical field of biological medicine, and relates to application of ABT-737 in preparation of anti-inflammatory drugs, in particular to application of ABT-737 in preparation of drugs for treating refractory rheumatoid arthritis. It is found for the first time that ABT-737 can induce apoptosis of inflammatory T cells derived from peripheral blood or synovial fluid of a patient suffering from the refractory rheumatoid arthritis, reduce the number of the inflammatory T cells and further inhibit occurrence and development of inflammation, and therefore ABT-737 has the prospect of being developed into drugs for treating the refractory rheumatoid arthritis and anti-inflammatory or immunomodulatory drugs.

The invention belongs to the technical field of biological medicines, and particularly relates to application of Venetoclax in preparation of an anti-inflammatory drug, in particular to a drug for treating rheumatoid arthritis (RA) with insufficient (MTX-IR) by using methotrexate. It is found for the first time that Venetoclax can induce apoptosis of inflammatory T cells of peripheral blood or synovial fluid of rheumatoid arthritis patients with insufficient curative effect on methotrexate, and occurrence and development of inflammation are inhibited by reducing the number of the inflammatory T cells, so that Venetoclax has the prospect of being developed into drugs for treating rheumatoid arthritis and anti-inflammatory and immunomodulatory drugs.

The invention provides a 3D synovial cartilage organ chip with a bionic structure and a preparation method and application thereof, and relates to the technical field of organ chip manufacturing. The organ chip comprises a main body; the main body comprises a top layer, a cartilage layer, a joint synovial fluid layer, a synovial membrane and an endothelial channel layer which are arranged in sequence; a cartilage cavity for accommodating articular cartilage cells is formed in the cartilage layer; a synovial fluid cavity used for containing joint synovial fluid is formed in the joint synovial fluid layer. A synovial membrane channel and an immune cell channel are arranged in the synovial membrane and endothelial channel layer in parallel; a permeation zone is arranged between the synovial membrane channel and the immune cell channel; a pressure cavity is formed in the top layer, and an elastic film is arranged on the portion, on one side of the cartilage cavity, of the pressure cavity. According to the scheme, mechanical stimulation is integrated into the organ chip, and in-vivo periodic compression during walking / activity of a human body can be reproduced, so that mechanical-biochemical coupling of a cartilage-synovial membrane-bone interface is simulated more truly, and the similarity of an in-vitro model is improved.

A surgical system includes a surgical generator and at least one energy delivery device. The surgical generator includes at least one energy output stage configured to deliver energy, a controller configured to control the energy output, and sensor circuitry. The energy delivery device(s) is coupled to the surgical generator, configured for insertion into a synovial joint, and configured to supply energy through synovial fluid in the synovial joint. In a sensing mode, the sensor circuitry is configured to sense at least one electrical parameter of the energy and the controller is configured to determine a parameter of the synovial fluid based thereon. The controller is further configured, in a treatment mode, to control the energy based upon the at least one determined parameter of the synovial fluid to treat tissue of the synovial joint.

The present disclosure is directed to a kit and methods for using the same. The kit includes a plate including at least one well, wherein the well includes a silver nitrate and a catalyst. The method includes providing a sample of synovial fluid to a well of a plate, wherein the well includes a silver nitrate and a catalyst and determining a presence of monosodium urate (MSU) crystals, wherein the presence of MSU crystals indicates that gout is detected in the sample.

The invention relates to a bone joint injection as well as a preparation method and application thereof. The bone joint injection comprises dopamine modified hyaluronic acid, amino-polyethylene glycol and a dissolving solution. The amino-polyethylene glycol is introduced into the dopamine modified hyaluronic acid, so that the micro-crosslinking effect can be enhanced, the mechanical property of the bone joint injection is improved, and the problems of joint abrasion and the like caused by lack of bone joint synovial fluid are remarkably improved.

The invention discloses a protein sample pretreatment kit and a proteome sample pretreatment method, the protein sample pretreatment kit comprises a reduction alkylation reagent, a protein extraction agent, a desalting reagent and an extractor, the amount of filler in the extractor is 0.8-1.5 mg, the amount of fixed polypeptide is 15-25 [mu] g, the amount of the protein extraction agent in the extractor is 0.5-1.5 mg, the amount of the protein extraction agent in the extractor is 0.5-1.5 mg, the amount of the protein extraction agent in the extractor is 0.5-1.5 mg, and the amount of the protein extraction agent in the extractor is 0.5-1.5 mg. During use, saliva, blood, cerebrospinal fluid, synovial fluid, clinical tissue samples, IP magnetic bead samples or electrophoretic band samples can be treated, the pretreatment difficulty is greatly reduced, the sample pretreatment difficulty is reduced, operation by non-professionals is facilitated, sample pretreatment becomes a standardized process, the process controllability and stability are remarkably improved, the reproducibility is improved along with improvement, and the method is suitable for popularization and application. Various types of samples can be treated, and clinical samples, IP magnetic bead samples, electrophoretic band samples and the like can be treated respectively.

Rheumatoid arthritis (RA) is the most common form of inflammatory rheumatism, affecting small joints, where swelling and pain reside, accompanied by structural damage that can lead to functional disability if no treatment is offered. The present inventors have shown that circulating MAIT cells are reduced and exhibit an activated and anti-apoptotic phenotype in rheumatoid arthritis patients compared with healthy controls. MAIT cell levels were also found to be increased in synovial fluid compared with peripheral blood, suggesting that the deficiency of circulating MAIT cells is due to migration of MAIT cells into joints. The severity of arthritis induced by methylated bovine serum albumin was reduced in mice from which MAIT cells had been ablated. This data suggests that MAIT cells are responsible for the exacerbation of arthritis. Thus, the present invention relates to the use of agents capable of inhibiting MAIT cell activation for the treatment of rheumatoid arthritis.

The invention discloses application of a synovial fluid source exosome miRNA marker in an osteoarthritis diagnosis product, and belongs to the field of biological medicine detection. The synovial fluid source exosome miRNA marker is miR-3196, and the reagents comprise reagents for detecting the expression level of the synovial fluid source exosome miRNA marker miR-3196 by a sequencing technology, a nucleic acid hybridization technology and a nucleic acid amplification technology. Experimental verification finds that miR-3196 is remarkably low in expression in a synovial fluid exosome of an OA patient, after the miR-3196 low in expression in the exosome is taken by cartilage cells, related signal channels are activated and degradation of the cartilage cells is promoted by regulating MAPK15 in the cartilage cells in a targeted mode, and the miR-3196 can be applied to early clinical diagnosis of osteoarthritis.

Compositions, methods, and kits are provided for diagnosing bacterial infections that distinguish tissue infected with dormant bacteria from uninfected tissue. In particular, the subject methods can be used to detect dormant bacteria, including bacteria in biofilms on joint replacement implants by measuring biomarkers in synovial fluid or blood or analyzing specific immune cell types, including regulatory T-cells, natural killer cells, monocytes, plasmacytoid dendritic cells, or myeloid dendritic cells within a periarticular tissue sample in contact with the effective joint space of an implant.

This invention relates to the field of lubricant technology, specifically to a diatomaceous earth@phospholipid microcapsule, its preparation method, and its uses. The diatomaceous earth@phospholipid microcapsule is a disc-shaped structure with a particle size of 20-30 micrometers. It is formed by covering the surface of a disc-shaped diatomaceous earth layer with a phospholipid bilayer, and filling the cavities of the disc-shaped diatomaceous earth with phospholipids. The diatomaceous earth@phospholipid microcapsule of this invention can exist relatively stably on the cartilage surface, forming a protective lubricating film. This not only prevents existing cartilage from being worn down, but also further improves the fluidity of synovial fluid, enhances the delivery of nutrients in the synovial fluid, and promotes the metabolic activity of chondrocytes, thereby promoting cartilage repair.

Rheumatoid arthritis (RA) is the most common form of inflammatory rheumatism involving small joints which are the seat for swelling and pain with structural damage, responsible for functional disabilities if no treatment is proposed. The inventors show that circulating MAIT cells were reduced and exhibited an activated and anti-apoptotic phenotype in RA patients compared to healthy controls. MAIT cell levels were also found to be increased in the synovial fluid as compared with the peripheral blood, suggesting that circulating MAIT cell deficiency is due to the migration of MAIT cells into the joint. Severity of arthritis induced by mBSA was reduced in mice depleted in MAIT cells. This data suggest that MAIT cells contribute to exacerbation of arthritis. Thus the present invention relates to the use of an agent capable of inhibiting the activation of MAIT cells for the treatment of rheumatoid arthritis

The invention provides injectable natural non-toxic edible hydrogel for treating osteoarthritis, which is prepared from the following substances in parts by weight: 15-35 parts of gilan gum, 20-40 parts of sodium carboxymethyl cellulose, 10-20 parts of calcium chloride and 60-120 parts of distilled water, the hydrogel takes natural polysaccharide as a matrix, and a three-dimensional network structure is formed through Ca < 2 + > ion crosslinking; according to the present invention, the injectable joint has excellent biocompatibility and degradability, the injectable joint can meet the joint cavity minimally invasive intervention requirement, the shear thinning characteristic (the viscosity during the injection is reduced) can ensure the smooth injection, the high elastic modulus is recovered in the body so as to effectively buffer the joint stress, and the porous structure loads the anti-inflammatory drug to achieve the local slow release; meanwhile, the surface lubricity simulates the synovial fluid function to reduce cartilage abrasion, all ingredients meet the food-grade standard, toxic and side effects are avoided, the preparation process is precise and controllable, and the composition is suitable for long-term repair and symptom management of osteoarthritis.

The invention discloses a PJI diagnosis model based on serum and synovial fluid indexes and a construction method of the PJI diagnosis model, and aims to solve the problems that in existing PJI diagnosis, specificity of a single index is insufficient, and misjudgment is easily caused due to culture negative. According to the method, serum and joint fluid samples and clinical information of all-joint replacement postoperative revision patients are collected, multiple types of clinical indexes are extracted after data processing and queue division, and eight core indexes are screened out by adopting LASSO logistic regression in combination with single-factor and multi-factor logistic regression; and constructing a visual column graph model containing a scoring system and a probability transformation formula through an R language. The model fully considers index weight and interaction, has the characteristics of low cost, easy acquisition and accurate diagnosis, is reliable in performance through training and verification queue verification, can assist clinicians in making scientific judgment before invasive treatment and reduce unnecessary detection and medical burden, and is suitable for popularization and application of medical institutions at all levels.

The application provides a traditional Chinese medicine preparation for dispelling wind and removing dampness and application, and relates to the technical field of traditional Chinese medicine preparation, wherein the traditional Chinese medicine preparation is composed of ten traditional Chinese medicines including Lonicera japonica, Spatholobus suberectus Dunn, silkworm sand, gentiana, safflower, persica, cortex fraxini, ardisia japonica, medicated leaven and honeycomb; the traditional Chinese medicine preparation can significantly reduce the swelling degree of ankle joint of an acute gouty arthritis model rat, reduce the expression level of serum TNF-alpha, IL-1beta and IL-6 inflammatory factors, and reduce the content of serum uric acid, creatinine and XOD, so as to achieve the effects of anti-inflammation and analgesia, reduction of blood uric acid, and the like; the clinical test results show that the traditional Chinese medicine preparation can reduce the pain degree of a patient, reduce the levels of inflammation related factors TNF-alpha and IL-1beta in synovial fluid of a knee joint of the patient, reduce the inflammation indexes WBC, ESR and CRP in serum, reduce the inflammation reaction of the patient's body, reduce the incidence of adverse reactions, and provide a new traditional Chinese medicine preparation with high safety and good curative effect for the treatment of acute attack of gout.

The invention belongs to the field of pet food processing, and relates to a premix for improving joint health of pets and a preparation method thereof. The premix is added with the following natural components: rhizoma polygonati, rhizoma drynariae, eucommia ulmoides, ligusticum wallichii, liquorice, turmeric, poria cocos and Chinese yam. The whole formula has the effects of tonifying liver and kidney to consolidate the root, promoting blood circulation to remove meridian obstruction to treat symptoms, tonifying spleen to remove dampness to assist transportation and transformation, tonifying spleen to prevent dampness stagnation, promoting diuresis to prevent positive qi injury, treating both symptoms and root causes, combining dredging and tonifying, and directly reaching the effects on joints. The premix can tonify cartilage, strengthen ligament, relieve pain, supplement synovial fluid, effectively improve the arthritis health of pets and improve the life quality of pets.

The invention provides a composition containing a plant extract and calcium and application of the composition to bone joint health. The composition can repair cartilage, promote secretion of bone joint lubricating liquid, remove inflammatory cells, inhibit inflammatory factors, delay cartilage degeneration, improve bone mineral density and bone strength, maintain bone mineral density, enhance bone firmness, improve osteoporosis and improve arthritis.