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64 results about "Biosensor" patented technology
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A biosensor is an analytical device, used for the detection of a chemical substance, that combines a biological component with a physicochemical detector. The sensitive biological element, e.g. tissue, microorganisms, organelles, cell receptors, enzymes, antibodies, nucleic acids, etc., is a biologically derived material or biomimetic component that interacts, binds, or recognizes with the analyte under study. The biologically sensitive elements can also be created by biological engineering. The transducer or the detector element, which transforms one signal into another one, works in a physicochemical way: optical, piezoelectric, electrochemical, electrochemiluminescence etc., resulting from the interaction of the analyte with the biological element, to easily measure and quantify. The biosensor reader device with the associated electronics or signal processors that are primarily responsible for the display of the results in a user-friendly way. This sometimes accounts for the most expensive part of the sensor device, however it is possible to generate a user friendly display that includes transducer and sensitive element (holographic sensor). The readers are usually custom-designed and manufactured to suit the different working principles of biosensors.
Intelligent evaluation system for endometrial receptivity based on biosensor data processing
This invention relates to the field of intelligent information processing technology and discloses an intelligent assessment system for endometrial receptivity based on biosensor data processing. The system acquires signals of multiple types of biomarkers in bodily fluid samples through multi-channel biosensor detection and performs structured preprocessing and quantitative transformation. Based on this, it establishes object-level associations between biosensor data and multi-source information to construct a unified feature data system. Furthermore, it constructs a weighted heterogeneous assessment graph and introduces a manifold stochastic feature modeling mechanism to generate manifold feature data. The manifold features and original features are jointly input into a shared-parameter cyclic Transformer model. Through cyclic inference and consistency constraints, multi-round feature fusion is achieved, outputting the endometrial receptivity assessment result. This invention improves the multi-source data modeling capability and the stability and consistency of the assessment process.
Owner:THE FIRST HOSPITAL OF LANZHOU UNIV +1
A liquid crystal biosensor based on functional nucleic acid regulation of CRISPR-Cas12a for high-sensitivity visual detection of protein markers and a preparation and detection method thereof
The application provides a liquid crystal biosensor based on functional nucleic acid regulation of CRISPR-Cas12a for high-sensitivity visual detection of protein markers and a preparation and detection method thereof. The sensor comprises a ready-to-use detection reagent and a nucleic acid molecule functionalized liquid crystal detection interface. The ready-to-use detection reagent comprises the following elements or components: a Cas enzyme-crRNA complex with shearing activity and an aptamer-activating sequence-two-dimensional material-based activating sequence locking system. The nucleic acid molecule functionalized liquid crystal detection interface is a DNA molecule or RNA molecule modified liquid crystal interface, wherein the nucleic acid aptamer specifically recognizes the target protein. The sensor of the application is simple and convenient to operate, does not require excessive manpower, has high sensitivity, and can realize semi-quantitative or quantitative detection.
Owner:INSTITUTE OF BASIC MEDICINE & CANCER CHINESE ACADEMY OF SCIENCES (PREPARATORY)
Paper-based semi-solid phase fret biosensor based on graphene oxide and dna fluorescent probe and preparation method thereof
Owner:ZHUHAI INST OF ADVANCED TECH CO LTD
An embedded whole-cell biosensor, its preparation method and application in machine learning assisted mercury ion detection
PendingCN122282714ALong-term fluorescence stabilityImprove mechanical propertiesSensor materialsBiocompatibility
This application discloses an embedded whole-cell biosensor, its fabrication method, and its application in machine learning-assisted mercury ion detection, belonging to the field of sensor materials and detection technology. This sensor encapsulates *E. coli* expressing green fluorescent protein (GFP) in a polyacrylamide / hyaluronic acid (PAAM / HA) hydrogel matrix, enabling highly selective and sensitive detection of mercury ions. It effectively eliminates interference from competing metal ions and can be completely regenerated through EDTA-2Na treatment, allowing for repeated use without performance loss. It maintains a stable fluorescence response for up to three weeks, possesses excellent biocompatibility and biodegradability, minimizing the risk of secondary contamination, and facilitates rapid on-site analysis.
Owner:OCEAN UNIV OF CHINA SHENZHEN RES INST +1
A method for monitoring the curative effect of Chinese and Mongolian and western medicines in real time based on biosensing technology
This invention relates to the field of medical data and discloses a method for real-time monitoring of the efficacy of traditional Chinese medicine, Mongolian medicine, and Western medicine based on biosensor technology. This method enables real-time monitoring of drug efficacy and personalized medication guidance. The process includes collecting real-time multimodal biosignals from the user's body surface or minimally invasive body fluid samples to obtain raw sensor signals and acquire sensor array output; extracting real-time pharmacokinetic characteristic signals based on a preset pharmacokinetic model and mapping them to efficacy assessment parameters according to drug type; comparing the efficacy assessment parameters with a preset safe and effective concentration range to generate real-time medication status indicators and dosage adjustment prompts. This invention designs quantitative assessment methods for different types of drugs, enabling real-time comparison to generate efficacy status codes and dosage adjustment suggestions. It comprehensively generates a medication guidance report and transmits it in real-time to the user or clinical monitoring end, allowing for immediate adjustment of the medication regimen and ensuring safe and effective medication.
Owner:AFFILIATED HOSPITAL OF INNER MONGOLIA MEDICAL UNIV (INNER MONGOLIA AUTONOMOUS REGION CARDIOVASCULAR INST)
His-tagged diversity nucleic acid aptamer and use thereof
The application discloses a histidine tag diversity nucleic acid aptamer and application thereof, and belongs to the technical field of biological processing. The nucleotide sequence of the histidine tag diversity nucleic acid aptamer is shown in SEQ ID NO. 3, or SEQ ID NO. 4, or SEQ ID NO. 5, or SEQ ID NO. 6. The application also discloses an application of the histidine tag diversity nucleic acid aptamer in preparation of a biosensor element for targeted recognition of a histidine tag-containing protein or polypeptide. The application also discloses an application of the histidine tag diversity nucleic acid aptamer in qualitative analysis, quantitative detection and separation and purification of the histidine tag-containing protein or polypeptide. The histidine tag diversity nucleic acid aptamer is obtained by taking HisA1-T63 aptamer as an initial chain, preliminarily improving the aptamer through sequence truncation, and repositioning a functional module to a loop region for diversity modification, thereby expanding the structural diversity of the histidine tag nucleic acid aptamer.
Owner:OCEAN UNIV OF CHINA
Kit and its preparation method and application, detection method of acetochlor
PendingCN122130943AMaterial analysis by observing effect on chemical indicatorFluorescence/phosphorescenceMedicineEngineering
This invention provides a reagent kit, its preparation method, and its application, as well as a method for detecting acetochlor, relating to the fields of analytical chemistry and biosensor technology. The reagent kit includes a detection solution and a test strip; the detection solution includes CuCo@PDA conjugated with an anti-acetochlor antibody; the test strip includes a sample pad, a detection pad, and an absorbent pad arranged sequentially; from the sample pad to the absorbent pad, the detection pad has a detection line and a control line arranged sequentially; the detection line is immobilized with an ATC-BSA conjugated antigen and protein-modified ATT-AuNCs; the protein includes BSA or ovalbumin; the control line is immobilized with a secondary antibody that specifically recognizes the anti-acetochlor antibody. This reagent kit utilizes the internal filtration effect to construct a dual-mode detection mechanism of "off" colorimetric signal / "on" fluorescence signal, and the signal can be collected and analyzed via a smartphone, making it suitable for rapid on-site screening of acetochlor residues in traditional Chinese medicinal materials.
Owner:BEIJING BACON MATERIA MEDICA TECH CO LTD
Neural microelectrodes with temperature control and temperature measurement functions and their fabrication method
ActiveCN116269410BTo control the temperatureRealize the control functionSensorsDiagnostic recording/measuringTemperature controlMicroelectromechanical systems
This disclosure provides a neural microelectrode with temperature control and temperature measurement functions, which can be applied in the field of microelectromechanical systems (MEMS) biosensor technology. The neural microelectrode includes: a substrate including a detection region; a microelectrode array disposed on the substrate and overlapping with the detection region, the microelectrode array being used to measure the electrical signal of a analyte; a temperature control element disposed on the substrate and overlapping with the detection region, used to control the temperature of the detection region; a temperature measurement element disposed on the substrate and overlapping with the detection region, used to measure the temperature of the detection region; and an insulating layer disposed on the microelectrode array, the temperature control element, and the temperature measurement element, covering the microelectrode array, the temperature control element, and the temperature measurement element. This disclosure integrates temperature control and temperature measurement functions with the neural microelectrode, realizing temperature control and temperature measurement of neurons, while simultaneously recording the electrical signals of neurons.
Owner:UNIV OF SCI & TECH OF CHINA
A data acquisition, monitoring, analysis and control system for use with a micro-hyperbaric chamber
This invention relates to the field of micro hyperbaric oxygen chamber technology, and discloses a data acquisition, monitoring, analysis, and control system for micro hyperbaric oxygen chambers, including: a multi-dimensional sensing module, a digital twin computing module, an adaptive pressure control module, and an active safety defense module. The system acquires multimodal data in real time through physical biosensors and visual devices, constructs a digital twin model using deep learning algorithms, and calculates physiological tolerance. Based on the physiological tolerance, it calls a nonlinear pressure boosting control model, automatically executing adaptive callbacks and dynamic pressure stabilization during pressure boosting to construct a physiological adaptation window. Simultaneously, by calculating a comprehensive risk index, it uses dual logic to determine whether the user is in a sleep or abnormal state, thereby executing wake-up and emergency pressure relief strategies. This invention solves the problem of eardrum damage caused by traditional linear pressure boosting and effectively avoids false alarms and missed alarms in safety monitoring, improving the comfort and safety of equipment operation.
Owner:OXYGEN HEALTH TECHNOLOGY (JIANGSU) CO LTD
Method for dynamic monitoring of microcystin-lr degradation based on molecular imprinting-carbon nanotube field effect transistor biosensor
The application discloses a method for dynamically monitoring microcystin-LR degradation based on a molecular imprinting-carbon nanotube field effect transistor biosensor, which in-situ polymerizes a polydopamine imprinting layer on a CNT-FET channel surface, fully utilizes the significant affinity between polydopamine and MC-LR and the high specific recognition of the molecular imprinting cavity to generate a synergistic effect, and converts the real-time fluctuation of the MC-LR concentration in the degradation process into a channel current signal instantaneously and without damage, so as to realize high-sensitivity, in-situ and bidirectional dynamic monitoring of the whole process of MC-LR degradation kinetics in a complex system.
Owner:XIANGTAN UNIV
A biosensor electrode for the directed modification of sarcosine oxidase and a method for its production and use
This application relates to a biosensing electrode with sarcosine oxidase-directed modification, its preparation method, and its application in the field of biosensing technology. The biosensing electrode includes: a metal electrode, and a DNA-enzyme composite nanostructure connected to the metal electrode; the DNA-enzyme composite nanostructure includes a DNA electron transport module and an enzyme catalysis module; the DNA electron transport module includes a DNA sheet structure and a mediator embedded in the DNA sheet structure; the enzyme catalysis module includes a sarcosine oxidase substrate complex, which is connected to the DNA sheet structure; the sarcosine oxidase substrate complex, the mediator-containing DNA sheet structure, and the metal electrode are spatially ordered in the sequence of sarcosine oxidase substrate complex – mediator-containing DNA sheet structure – metal electrode. This biosensing electrode, under the condition of introducing a mediator, can improve the electron transport rate, thus exhibiting a good signal response during sarcosine testing.
Owner:GUANGZHOU NAT LAB +1
Method for preparing microfluidic sensor for myocardial infarction marker detection and application thereof
PendingCN122150356ADecorative surface effectsMaterial analysis by electric/magnetic meansSignal responseCardiac muscle
The application provides a microfluidic sensor preparation method and application for myocardial infarction marker detection, belongs to the technical field of biosensors, realizes rapid, convenient and accurate detection of cTnI by organically combining photoelectrochemical detection technology, immunological recognition technology and a microfluidic chip platform, has high signal response, good repeatability and excellent anti-interference ability, provides a detection platform with broad application prospect for early diagnosis and dynamic monitoring of myocardial injury, and effectively solves the core pain points of complicated manual operation and long detection period in conventional immunological analysis, thereby providing a highly adaptive technical platform for rapid screening in a clinical scene.
Owner:SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
A water pressure intelligent adaptation method and system for a water flosser
This invention relates to the field of biosensor technology, and discloses a method and system for intelligent water pressure adaptation of a water flosser based on integrated sensing. The method includes: determining the cleaning area of the target oral cavity for the water flosser; analyzing the health of the cleaning area based on gingival tissue state data, and analyzing the cleaning needs of the cleaning area based on oral pressure data; combining health and cleaning needs, and analyzing the initial water pressure value of the cleaning area using a preset area-state-water pressure mapping model; collecting the subconscious physiological reaction signals of the oral cavity user corresponding to the initial water pressure value, and analyzing the comfort coefficient of the oral cavity user based on the subconscious physiological reaction signals; optimizing the initial water pressure value based on the comfort coefficient to obtain a target water pressure value, and performing intelligent water pressure adaptation of the water flosser to the target oral cavity based on the target water pressure value. This invention can improve the cleaning effect for oral cavity users.
Owner:深圳科士洁科技有限公司
A carbon dot probe for detecting parathyroid hormone and a preparation method and application thereof
This invention belongs to the field of biosensor detection technology, specifically disclosing a carbon dot probe for detecting parathyroid hormone, its preparation method, and its application. The carbon dot probe provided by this invention comprises carbon dots and monoclonal antibodies 1 and 2 covalently coupled to the carbon dot surface. Monoclonal antibodies 1 and 2 specifically recognize the N-terminal and C-terminal epitopes of the parathyroid hormone molecule, respectively. The carbon dots possess aggregation-induced emission (AIE) properties and photosensitivity. This invention's carbon dot probe can achieve a highly sensitive, highly specific, rapid, and dual-mode visual detection method for parathyroid hormone by detecting the dual signal changes of aggregation-induced emission fluorescence enhancement and photocatalytic TMB color development induced by PTH-induced specific aggregation. This invention also provides carbon dots with aggregation-induced emission properties and photosensitivity, as well as a method for preparing the carbon dot probe for detecting parathyroid hormone.
Owner:CENT SOUTH UNIV
Cu-BTC / CNT-cellulose conductive hydrogel for ascorbic acid detection and its preparation method
PendingCN122127624AImprove electrochemical activityImprove sensor sensitivityNanofiberComposite nanoparticles
This invention relates to the field of sensing materials technology, specifically disclosing a cellulose-based conductive hydrogel material for ascorbic acid detection, its preparation method, and its applications. This hydrogel uses TEMPO oxidized cellulose nanofibers (TOCNF) as a biocompatible matrix. By introducing Cu-BTC / CNT composite nanoparticles, a three-dimensional conductive network with both high electrochemical activity and structural stability is constructed. While retaining the biocompatibility and flexibility of cellulose, it exhibits particularly high sensitivity and selectivity in detecting small biomolecules such as ascorbic acid, with short response time and good stability. This method achieves synergistic optimization of the composite material's structural uniformity and functionality without relying on complex molding processes. Experimental results show that the conductive hydrogel prepared by this invention possesses good mechanical properties and biocompatibility, exhibiting high sensitivity and stability in biosensing fields such as ascorbic acid detection, providing a novel high-performance material basis for flexible biosensors.
Owner:TIANJIN UNIV OF SCI & TECH
Arsenic ion synthetic biosensor based on cell-free system and application thereof
The application discloses a paper-based arsenic ion synthetic biosensor based on a cell-free system and application thereof, and comprises a cell-free arsenic ion sensing module and a semi-quantitative paper-based detection chip. The cell-free arsenic ion sensing module is composed of a synthetic arsenate reduction module, an arsenic ion specificity sensing module, a high-gain transcription amplification module and a visual reporting module connected in sequence. The synthetic arsenate reduction module converts pentavalent arsenic into trivalent arsenic; the arsenic ion specificity sensing module recognizes trivalent arsenic and removes transcription inhibition; the high-gain transcription amplification module amplifies the signal through ecf11 cascade; and the visual reporting module outputs a colorimetric or fluorescent signal. The application integrates an engineered promoter and an ecf11 cascade amplification system in a cell-free system, greatly improves sensitivity and dynamic range and keeps low leakage. The paper-based chip can present different pattern arrangements according to arsenic concentration and is easy to read. The application provides an environmentally friendly, portable and easy-to-read innovative method for monitoring arsenic pollution in drinking water.
Owner:ZHEJIANG UNIV
Biosensor and method for manufacturing the same
The present application relates to a kind of biochip and its manufacturing method and application.The biochip includes: substrate;Multiple pixel units are arranged on the substrate, and each pixel unit includes the microelectrode array with working electrode, counter electrode and dielectric structure;Wherein, the surface of the dielectric structure has surface modification layer, and the surface modification layer includes interlinked first surface modification structure and second surface modification structure;The first surface modification structure includes the alkoxysilane modification layer bonded with the surface of the dielectric structure and the functional modification layer connected with the alkoxysilane modification layer by sulfide bond;And the second surface modification structure includes the alkoxysilane modification layer bonded with the surface of the dielectric structure.The biochip surface has region-selective and functionally differentiated functional structure, and it is of great significance to promote the development of high-throughput, programmable and strong bio-compatible chip technology.
Owner:SUZHOU SIJI BIOTECHNOLOGY CO LTD
One-step monoclonal antibody electrochemical detection biosensor and preparation method and application thereof
The application discloses a one-step monoclonal antibody drug electrochemical detection biosensor and a preparation method and application thereof. The application designs and screens an affinity specific DNA aptamer hairpin probe and uses the same as a specific capture unit, then uses a gold-sulfur bond to fix the DNA aptamer hairpin probe on a screen-printed electrode surface, meanwhile, uses a self-assembly interaction between a metal and a mercapto group to fix a blocking agent introduced on the screen-printed electrode surface, and blocks the excess electrode surface reaction sites, so that the one-step monoclonal antibody drug electrochemical detection biosensor is obtained. The one-step monoclonal antibody drug electrochemical detection biosensor has the advantages of simple operation, high sensitivity, less non-specific adsorption, high capture efficiency, stable physical and chemical properties, strong specificity and good selectivity, and can be used for capturing antibody drugs in complex biological samples.
Owner:JINAN UNIVERSITY
A bmo r mutant responding to r,r-2,3-butanediol in a broad range and application thereof
This invention belongs to the field of bioengineering technology, specifically relating to a method for... R,R BmoR protein mutants with a wider detection range (0-200 mM) for 2,3-butanediol, and their applications in... R,R Applications in the detection or biosensor of 2,3-butanediol. The BmoR protein mutant was obtained by mutating the wild-type BmoR protein shown in SEQ ID NO.1 with the N89D mutation, and has the amino acid sequence shown in SEQ ID NO.3. The N89D mutant... R,R -2,3-Butanediol has a wider detection range (0-200 mM), solving the problem of the inability to screen wild-type BmoR proteins. R,R The problem of high-yield strains of 2,3-butanediol can be addressed by screening and applying strains with even higher yields.
Owner:BEIJING INST OF TECH
Electrochemiluminescence-based lateral flow immunochromatographic membranes and biosensors
This invention belongs to the field of biodetection technology and provides a lateral flow immunochromatographic membrane and biosensor based on electrochemiluminescence. The lateral flow immunochromatographic membrane includes a membrane body, a wax layer fixedly coated on the surface of the membrane body, control lines, and conductive electrodes, as well as detection lines fixedly coated on the surface of the conductive electrodes. The wax layer has lateral flow channels arranged along the length of the membrane body. The conductive electrodes span the lateral flow channels, with their two ends fixedly coated on the surface of the wax layer. The conductive electrodes and control lines are spaced apart along the length of the membrane body. The lateral flow immunochromatographic membrane and biosensor provided by this invention combine qualitative screening with visualized results and high-sensitivity quantitative analysis, offering simple operation, short detection time, and improved detection efficiency and reliability.
Owner:YUNNAN NORMAL UNIV
Methods and apparatus for high-resolution spatial and temporal mapping of large-scale electrophysiological dynamics and transcriptomic profiles within intact brain tissue
This invention relates to an in vitro method for mapping the spatiotemporal electrophysiological dynamics and spatial transcriptional profiles of cells in a functional neuronal cell ensemble. This in vitro method includes simultaneously recording and analyzing spatial data of molecular activity and electrical network activity at the level down to individual cells using high-density electrobiosensors, spatial transcriptomics, optical imaging, and advanced computational strategies. The invention also relates to: methods for identifying the composition of a functional neuronal ensemble; methods for monitoring the spatiotemporal electrophysiological dynamics and transcriptional profiles of cells in a functional neuronal cell ensemble; methods for monitoring the cellular composition of a functional neuronal ensemble; methods for identifying compounds that influence spatial electrophysiological and transcriptional dynamics; and / or methods for identifying the cellular composition of a functional neuronal cell ensemble based on detected dynamics; and devices thereof. This invention allows for a better understanding of disease mechanisms, the identification of therapeutic targets, and the development of new drugs and treatment regimens.
Owner:GERMAN CENT FOR NEURODEGENERATIVE DISEASES
Nanowire Field Effect Transistor Biosensor with Ultra-High Sensitivity Detection Mechanism for Medical Purposes
Nanowire Field Effect Transistor Biosensor with Ultra-High Sensitivity Detection Mechanism for Medical Purposes
Owner:KISHORE CHANDRA SINGH +6
A near single-molecule limit quantum biosensor and detection method
This invention discloses a near-single-molecule-limit quantum biosensor and detection method. By integrating a notched coplanar microwave waveguide, a plasma-enhanced gold nanoisland array, and a semi-arc-shaped framework chaotic hybrid microfluidic structure on a single chip, it achieves a high degree of integration of optical, microwave, and fluid reaction systems, improving mixing efficiency and reaction kinetics under microfluidic conditions, reducing diffusion limitations, and increasing capture efficiency, thereby shortening detection time. It enables rapid quantitative detection of atmolar-level miRNAs and has multiplex detection capabilities. The plasma nanogap formed by the gold nanoislands and gold-silver core-shell nanostructures can enhance the contrast of NV center fluorescence readout and ODMR, improve the signal-to-noise ratio, and significantly improve the detection limit and quantitative stability of ultra-low abundance miRNAs.
Owner:SOUTHEAST UNIV +1
An electrochemiluminescence sensor, its preparation method and application
This invention relates to the field of biosensor technology, and in particular to an electrochemiluminescence sensor, its fabrication method, and its applications. The sensor comprises a three-electrode system, wherein the working electrode includes a substrate electrode, an amino-functionalized vertically ordered mesoporous silica film formed on the surface of the substrate electrode, and nitrogen-doped graphene quantum dots confined within the amino-functionalized vertically ordered mesoporous silica film; the electrolyte used in the sensor is a phosphate buffer containing luminol. This invention achieves highly sensitive and selective detection of superoxide dismutase (SOD) by constructing an electrode modified with nitrogen-doped graphene quantum dots confined within an amino-functionalized vertically ordered mesoporous silica film, utilizing its enhanced luminol-dissolved oxygen electrochemiluminescence signal and the specific quenching effect of SOD on superoxide anions.
Owner:ZHEJIANG SCI-TECH UNIV
A porphyrin covalent organic framework@polyindole pentamethylic light anode material and a preparation method and application thereof
This invention relates to a photoanode material based on a porphyrin covalent organic framework@polyindolepentaformaldehyde, its preparation method, and its application, belonging to the field of photoelectrochemical biosensor technology. This invention uses Dha and Tph to synthesize D-T-COF via a solvothermal method, and modifies it onto the surface of an FTO conductive substrate; further, P5FIn is deposited via electropolymerization to form a uniform and widely distributed layer on the D-T-COF surface. p‑n Heterogeneous junction structure. Based on D-T-COF@P5FIn composite material and p-tau217 capture antibody, an opto-chemical immunosensor array capable of accurately identifying trace amounts of p-tau217 in human plasma was constructed. It has achieved significant breakthroughs in sensitivity, specificity and practicality, and is particularly suitable for the reliable detection of extremely low concentrations of biomarkers in complex biological matrices, showing broad prospects for clinical translational applications.
Owner:SHENYANG PHARMA UNIV
A material for high-selectivity detection of alkaline phosphatase and a preparation method and application thereof
This invention discloses a material for highly selective detection of alkaline phosphatase, its preparation method, and its applications, belonging to the field of analytical chemistry. The material for highly selective detection of alkaline phosphatase comprises a phosphate-decomposed framework and laccase encapsulated within the framework. The material of this invention eliminates the need for exogenous H2O2 and peroxidase, utilizing framework encapsulation to achieve controlled "encapsulation-release" of laccase, thereby realizing in-situ cascade catalytic amplification. The proposed dual-product (phosphate and phenol) synergistic gating strategy effectively eliminates false positive signals caused by endogenous phosphate or phenolic interferences, providing a universal framework for designing highly selective and sensitive biosensors, and overcoming the fundamental defect of poor accuracy in complex samples in traditional direct coupling methods.
Owner:CHENGDU UNIV
IoT-based biosensor-based platelet-rich plasma concentration detection system
PendingCN122084726AMaterial analysis by electric/magnetic meansLaboratory glasswaresNerve networkWhite blood cell
This invention relates to the interdisciplinary field of biomedical detection and Internet of Things (IoT) sensing technology, specifically disclosing a platelet-rich plasma concentration detection system based on IoT biosensors. The system includes a microfluidic sensor chip, a multi-frequency electrochemical impedance spectroscopy (MES) acquisition unit, a cell morphology identification module, an IoT communication unit, and a remote data processing server. It monitors platelet dynamic aggregation in real time through a three-electrode system, and combines high-dimensional features of broadband impedance spectroscopy with a pre-trained convolutional neural network model to accurately distinguish between simple platelet aggregates, platelet-leukocyte complexes, and microthrombus structures. Furthermore, it leverages the IoT architecture to complete encrypted data upload, remote model optimization, and intelligent coagulation risk assessment. Through the above technical solutions, this invention improves the sensitivity, specificity, and clinical applicability of the detection, constructing a closed-loop blood monitoring system from bedside detection to cloud-based decision support.
Owner:ZHUHAI LONGTIME BIOLOGICAL TECH CO LTD +1
Flexible electrode array, system and method of manufacturing for closed-loop neuromodulation
This invention discloses a flexible electrode array, system, and manufacturing method for closed-loop neural modulation, relating to the fields of neural engineering, medical devices, and biosensor technologies. The flexible electrode array includes a flexible biocompatible substrate, a recording electrode array, and a stimulating electrode array. The recording sites are arranged in a matrix, and the stimulating sites are coplanar with the recording sites, located between or within rows of the recording electrode array, achieving a spatially co-located layout of recording and stimulation. The system also includes an external control unit for receiving neural signals acquired by the recording electrode array and dynamically adjusting the stimulation parameters applied to the stimulating electrode array based on the neural signals. This application improves biocompatibility, achieves high-resolution signal mapping, and enables efficient closed-loop neural modulation by shortening the spatiotemporal distance between stimulation and monitoring.
Owner:SHANGHAI JINNAO MEDICAL TECHNOLOGY CO LTD
A naked eye visualization method for mycobacterium tuberculosis detection
The present application relates to a kind of naked eye visualization method for Mycobacterium tuberculosis detection.The nucleic acid detection biosensor based on target nucleic acid recognition is constructed to form toehold-containing three-way junction (TWJ) and induce multi-cycle exponential amplification, cascade CRISPR / Cas14a magnetic separation enhanced gold nanometer colorimetric nucleic acid detection.The CRISPR-Cas14a transcleavage biotinylated oligonucleotide chain (biotin-S1) is involved, and magnetic bead complex MB-S1 is used to capture oligonucleotide chain S2 modified gold nano (AuNP-S2) to realize its magnetic pull-down;Characteristic process involves target nucleic acid induction TWJ formation and isothermal exponential amplification, its product is recovered target by toe mediated strand displacement, realizes multi-cycle exponential amplification;Product ssDNA activates CRISPR / Cas14a transcleavage biotin-S1, releases gold nano probe, and supernatant after magnetic separation is used for naked eye visual detection;Involved specific three-way junction primer and template for specific target Mycobacterium tuberculosis 16S rDNA, and toehold-containing three-way junction mediated multi-cycle exponential amplification and CRISPR / Cas14 assisted magnetic separation enhance AuNP colorimetric for Mycobacterium tuberculosis nucleic acid macroscopic visual detection formula.
Owner:重庆医科大学国际体外诊断研究院
Rigid-response fluorescence polarization biosensor for detecting aflatoxin b1 and application thereof
The application discloses a rigid response fluorescence polarization biosensor for detecting AFB1 and application. The rigid response fluorescence polarization biosensor comprises a target recognition unit, the target recognition unit comprises an aptamer-complementary chain double-stranded complex which specifically recognizes AFB1; a signal amplification unit, the signal amplification unit comprises a rolling circle amplification reaction system; a signal conversion unit, the signal conversion unit comprises a CRISPR / Cas12a detection system; and a signal output unit, the signal output unit comprises a conformational restriction depolarization reporter, and the conformational restriction depolarization reporter is a single-stranded DNA / double-stranded DNA complex structure. The application realizes quantitative detection of AFB1 by using FP value change, has the advantages of low detection limit, strong specificity, strong anti-matrix interference capacity, simple operation and the like, and can be widely applied to rapid and high-sensitivity detection of AFB1 in complex samples such as grains, foods and feed.
Owner:JIAXING UNIV