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A biosensor is an analytical device, used for the detection of a chemical substance, that combines a biological component with a physicochemical detector. The sensitive biological element, e.g. tissue, microorganisms, organelles, cell receptors, enzymes, antibodies, nucleic acids, etc., is a biologically derived material or biomimetic component that interacts, binds, or recognizes with the analyte under study. The biologically sensitive elements can also be created by biological engineering. The transducer or the detector element, which transforms one signal into another one, works in a physicochemical way: optical, piezoelectric, electrochemical, electrochemiluminescence etc., resulting from the interaction of the analyte with the biological element, to easily measure and quantify. The biosensor reader device with the associated electronics or signal processors that are primarily responsible for the display of the results in a user-friendly way. This sometimes accounts for the most expensive part of the sensor device, however it is possible to generate a user friendly display that includes transducer and sensitive element (holographic sensor). The readers are usually custom-designed and manufactured to suit the different working principles of biosensors.
Health state assessment method and system based on multi-modal biosensor
The invention discloses a health status assessment method and system based on a multi-modal biosensor, and the method comprises the steps: synchronously collecting a plurality of physiological signals of a plurality of target species objects, and generating a multi-modal physiological data flow with consistent time and space; inputting the multi-modal physiological data stream into a deep learning model, and outputting a high-dimensional feature vector fused with time-space characteristics; inputting the high-dimensional feature vector into a cross-species adaptation model based on transfer learning, and outputting a cross-species universality state vector representing the current health abnormal state and confidence of the target object; inputting the cross-species universality state vector into a lightweight risk assessment model, and outputting a quantitative assessment result reflecting the instant health risk level of the target object; and according to a quantitative evaluation result, automatically generating and triggering a personalized health intervention instruction for the target object. By utilizing the embodiment of the invention, the physiological difference of different species can be dynamically adapted, and the accuracy and the universality of health state evaluation are improved.
Owner:HANGZHOU XIAOXIANG ZHIYUAN TECHNOLOGY CO LTD +1
AI and password technology fused identity authentication method
ActiveCN120301605AKey distribution for secure communicationUser identity/authority verificationBiometric dataKey (cryptography)
The invention relates to the technical field of intelligent security and protection, and discloses an identity authentication method integrating AI and password technologies. According to the method, biological characteristic data are collected through a multi-mode biosensor, and the biological characteristic data are encrypted after being processed by a deep convolutional neural network. And training an identity authentication model by using a federated learning framework in combination with a differential privacy technology, and carrying out distributed verification. And generating a one-time dynamic password based on a verification result, binding the one-time dynamic password with spatio-temporal characteristics of user behaviors to construct a matrix, and controlling the access authority by adopting an attribute-based encryption algorithm. The system is also provided with an abnormal behavior detection model, a dynamic identity label updating mechanism, a secret key updating strategy and a multi-factor authentication decision engine. The method improves the security and accuracy of identity authentication, realizes fine authority management, and is suitable for the field of intelligent security and protection.
Owner:JIANGSU IDEABANK MICROELECTRONICS TECH
Space thermal microclimate multi-target regulation and control method, device and system, medium and product
PendingCN120863288AAir-treating devicesSeat heating/ventillating devicesOptical radiationThermal comfort
The invention relates to the crossing field of biothermodynamics and dynamic environment control, and discloses a space thermal microclimate multi-target regulation and control method, device and system, a medium and a product. The method comprises the following steps: acquiring physiological parameters (including effective dressing thermal resistance and local sweat gland density of posture correction) of a passenger, environment space-time parameters and light environment parameters through a biological characteristic sensor, an environment sensor and an optical sensor; constructing a dynamic human body thermal comfort model library, and adopting a feature coding-parameter decoding mechanism to match a target thermal comfort model; generating a hierarchical regulation strategy based on the difference between the local comfortable temperature and humidity interval and the real-time thermal environment sensing parameter; and the refrigerant circulation system, the fluid circulation system and the optical radiation system are cooperatively controlled to realize local microclimate regulation and control. According to the method, the problems of inaccurate thermal comfort modeling and low multi-system cooperation efficiency in a transient environment are solved, the energy efficiency ratio is improved, and the comfort of passengers in a cabin is remarkably improved.
Owner:CHONGQING CHANGAN AUTOMOBILE CO LTD
Early warning method and system based on marine ranch pollution
The invention relates to an early warning method and system based on marine ranch pollution, and relates to the technical field of signal processing, and the method comprises the steps: building a three-dimensional biosensor network matrix, and dynamically adjusting the sensor deployment density of a marine ranch; a biological indicator response function is constructed according to the sensor deployment density, and a comprehensive response value is calculated; performing multi-source fusion on the sensor network data, the biological response data and the traditional monitoring data through a spatial weight kernel function to obtain a fused pollution index; calculating and evaluating the risk index of the comprehensive pollution risk of the marine ranch by combining the fused pollution index and the space-time parameter; dynamically optimizing the early warning threshold according to the change rate of the risk index and the historical data entropy to obtain a dynamic threshold; and based on the logarithmic relationship between the risk index of the marine ranch and the dynamic threshold, generating a multi-stage early warning signal for marine ranch pollution early warning. The accuracy of marine ranch pollution early warning can be improved.
Owner:GUANGDONG OCEAN UNIVERSITY
Omnibearing intelligent cardiopulmonary health management system and method and electronic equipment
PendingCN120072357APhysical therapies and activitiesMedical communicationEmergency medicineMedical emergency
The invention relates to the technical field of health management and medical information, and discloses an all-dimensional intelligent cardiopulmonary health management system and method and electronic equipment, and the system comprises a biological sensing module which is used for continuously collecting key physiological indexes of the heart rate, the oxyhemoglobin saturation, the respiratory rate and the motion state of a user; the data processing module is used for storing the collected physiological data and historical exercise records of the user through a central database of a cloud computing platform; the deep learning analysis module is a prediction model built based on a deep learning framework and is used for analyzing the physiological data of the user and generating a personalized exercise plan; the real-time monitoring module is used for detecting the physiological state of the user and automatically adjusting the exercise intensity or type when the physiological state exceeds a preset threshold value. The technical scheme that the multi-mode biosensor is combined with the real-time monitoring module is adopted, and the technical effect of comprehensively monitoring the cardiopulmonary function state of the user in real time is achieved.
Owner:北京一石科技有限责任公司
Nucleic acid mediator assisted ultrasensitive CRISPR biosensor
The present invention relates to CRISPR / Cas based biosensing materials, assays, and methods. Specifically, the present technology relates to CRISPR / Cas-based ultra-sensitive detection methods for nucleic acid assays using specific molecular constructs, including constructs referred to as nucleic acid mediators, comprising single-stranded and double-stranded nucleic acid sequences of a cyclic conformation, and palindromic oligonucleotides. The materials and methods according to the invention can also be used to enhance the sensitivity of existing bioassays.
Owner:NEWSOUTH INNOVATIONS PTY LTD
Preparation of heterojunction dual-photoelectrode type photoelectrochemical sensor based on multi-ball cascade DNA reaction amplification
The invention belongs to the technical field of immunoassay and biosensing, and provides a novel construction mode of a heterojunction dual-photoelectrode photoelectric sensing strategy and a preparation method of an in-situ heterojunction MIO photocathode. The invention relates to a preparation method of a heterojunction dual-photoelectrode type photoelectrochemical sensor based on multi-ball cascade DNA reaction amplification. Specifically, the design is composed of an In2O3 / CdS photoanode and an in-situ formed MIL-68 / In2O3 (MIO) photocathode. The heterojunction dual-photoelectrode sensing strategy effectively prolongs an electron-hole transmission path, enhances photo-generated charge separation, and realizes' super-open 'PEC signal output, thereby providing a steady baseline for signal conversion. Meanwhile, a multi-sphere cascade DNA reaction is combined to output a three-dimensional DNA net structure to provide a large number of sites for embedding of a PEC quenching probe doxorubicin-ferrocene carboxylic acid (DOX-FcCOOH), obvious PEC signal quenching is realized by utilizing the efficient competitive electron transfer capability of the three-dimensional DNA net structure, and finally, a signal conversion system with'opening-super opening-closing 'multistage response characteristics is constructed. The detection of the nucleic acid marker Pax-5a gene of the B cell acute lymphatic leukemia is realized by detecting target objects with different concentrations.
Owner:SHANDONG UNIV OF TECH
Internet of Things monitoring and early warning system and method for lake water quality
PendingCN120761596AParticular environment based servicesTesting waterEnvironmental resource managementThe Internet
The invention relates to an internet-of-things monitoring and early warning system for lake water quality. The internet-of-things monitoring and early warning system comprises a multi-mode intelligent sensor acquisition system, a self-adaptive wireless transmission system, a distributed data storage system, an intelligent data analysis system, a visual interaction system, an intelligent early warning system and a remote intelligent control and repair system. The invention also relates to a monitoring method using the lake water quality Internet of Things monitoring and early warning system. Traditional and innovative sensors are cooperated to comprehensively collect water quality and ecological data, the biosensor accurately detects specific pollutants, the hyperspectral imaging sensor monitors algae distribution and water color change, rich and high-precision data are provided for lake water quality monitoring, the monitoring comprehensiveness and precision are improved, and the water quality monitoring system is suitable for being popularized and applied. The lake ecological protection work is powerfully supported.
Owner:HUBEI PROVINCIAL WATER RESOURCES & HYDROPOWER PLANNING SURVEY & DESIGN INST
Dynamic adaptation method and device for display content of LED screen and medium
ActiveCN120340408AStatic indicating devicesAcquiring/recognising facial featuresReal time analysisEngineering
The invention discloses a dynamic adaptation method and device for display content of an LED screen and a medium, and the method comprises the steps: collecting original feature data of audiences in a radiation range of the LED screen through a multi-mode biosensor, carrying out the preprocessing of the original feature data, and generating a desensitization feature vector through local anonymization processing; performing real-time analysis on the desensitization feature vector by using a multi-modal fusion model in an edge computing node to generate a primary emotion tag; uploading the primary emotion label and the associated historical behavior data of the audience to a cloud platform so as to predict potential interests of the audience through a preset emotion deep analysis model, and generating a dynamic content adaptation strategy in combination with the potential interests of the audience; on the basis of a dynamic content adaptation strategy, display content matched with the emotion label is called from a pre-stored material library, low-delay rendering is conducted on the display content, and color parameters and the content display sequence of the LED display screen are dynamically adjusted according to the emotion analysis result.
Owner:SHANDONG INSPUR ULTRA HD INTELLIGENT TECH CO LTD
Method and device for quickly and nondestructively detecting quality of aquatic products
ActiveCN120741364ABiological modelsColor/spectral properties measurementsBiochemical engineeringFood safety
The invention provides a rapid nondestructive quality detection method and device for aquatic products, and relates to the technical field of food safety detection, and the device comprises a spectral analysis module, a biosensing module, an electrochemical detection module, a data processing module, a data output module and a portable device. According to the rapid nondestructive quality detection method and device for the aquatic products, multi-index synchronous detection of freshness, drug residues and heavy metal content of the aquatic products is realized through fusion of spectral analysis, biosensing and electrochemical detection technologies.
Owner:SOUTH CHINA NORMAL UNIV
Mask-based testing system for detecting biomarkers in exhaled breath condensate, aerosols and gases
ActiveUS12442726B2Withdrawing sample devicesPreparing sample for investigationAnalyteBiologic marker
A mask-based testing system for detecting a biomarker received from lungs and airways of a test subject includes an exhaled breath condensate (EBC) collector integrated into an inside of a face mask worn by the test subject. The EBC collector converts breath vapor received from the lungs and airways of the test subject into a fluid biosample. A biosensor is fixed to the inside of the face mask for receiving a fluid biosample from the EBC collector and testing the fluid biosample for a target analyte. The biosensor generates a test signal dependent on at least the presence and absence of the target analyte in the fluid biosample. An electronic circuit is fixed to an outside of the mask for receiving the test signal, determining from the test signal a test result signal depending on detecting or not detecting the target analyte, and transmitting the test result signal to a remote receiver.
Owner:DANIELS JOHN J
Method for manufacturing implantable micro-biosensor
A method for manufacturing an implantable micro-biosensor includes the steps of: a) providing a substrate, b) forming on a first surface of the substrate a first working electrode which at least includes a first sensing section including a first conductive material, c) forming on the first surface of the substrate at least one second working electrode which at least includes a second sensing section disposed proximate to at least one side of the first sensing section, and d) forming a chemical reagent layer which at least covers the first conductive material of the first sensing section and which reacts with the analyte to produce a product.
Owner:BIONIME
Pneumatic actuator remote intelligent monitoring system based on Internet of Things
The invention discloses a pneumatic actuator remote intelligent monitoring system based on the Internet of Things, and relates to the technical field of actuator control. Traditional, quantum and biosensors are used for data acquisition, adaptive coding is adopted, transmission is combined with quantum encryption and ultra-wideband, quantum machine learning and causal inference are analyzed and applied, and remote control comprises brain-computer, holographic and intelligent agent technologies; naked-eye 3D and AR are used for visualization, system management depends on a block chain and a quantum key, and a self-repairing function is achieved. The advanced technology is creatively applied, data are comprehensively collected, transmission safety is guaranteed, faults are accurately analyzed and predicted, convenient remote control and visual display are achieved, system safety and stability are improved, and efficient operation of the pneumatic actuator is powerfully guaranteed.
Owner:LIAONING YUANLU MASCH EQUIP MFG CO LTD
Method for improving L-tryptophan synthesis level in escherichia coli based on ARTP mutagenesis high-throughput screening
The invention discloses a method for improving the synthesis level of L-tryptophan in escherichia coli based on ARTP mutagenesis high-throughput screening, and belongs to the technical field of bioengineering. According to the invention, the L-tryptophan biosensor pSensor-trp3 is constructed, and the L-tryptophan biosensor pSensor-trp3 is good in response under the condition that the exogenous addition concentration range of L-tryptophan is 0-300mg. L <-1 >. High-throughput screening is performed by using a flow cytometry, a dominant mutant strain YB-2 is obtained through iterative mutagenesis, and the shake flask L-tryptophan yield and the sugar-acid conversion rate of the dominant mutant strain YB-2 are 5.68 g.L <-1 > and 0.103 g.g <-1 > respectively and are increased by 11.4% and 10.7%. Through second-generation whole genome re-sequencing comparative analysis, it is speculated that the genes ynfB and waaO possibly have a promoting effect on synthesis of L-tryptophan. The L-tryptophan biosensor used in the invention also provides reference for high-throughput screening and transformation of other related L-tryptophan production strains.
Owner:JIANGNAN UNIV
Preparation method and application of photo-thermal biosensor for tumor cell quantification and vitality double-index liquid biopsy
The invention discloses a preparation method and application of a photo-thermal biosensor for tumor cell quantification and vitality dual-index liquid biopsy. Phosphatidylserine (PS) is exposed by enriching and cracking tumor cells, is identified and combined by an aptamer Apt2, and is driven to be separated from Apt2-Cu2-xTe NSs; after magnetic separation, separated supernatant is subjected to photo-thermal detection; the photo-thermal signal intensity is positively correlated with the number of cells, so that quantitative detection is realized; during cell apoptosis, PS is turned out of the membrane, Apt2-Cu2-xTe NSs is combined with the surface of the membrane, separated supernatant is subjected to photo-thermal detection after magnetic separation, and the photo-thermal signal enhancement amplitude is in negative correlation with cell activity. The platform has the advantages of POCT characteristics, low cost and portability, and a new tool is provided for precise tumor diagnosis and treatment.
Owner:NINGBO UNIV
Intelligent drug delivery pain management bracelet
The invention discloses an intelligent drug delivery pain management bracelet, and relates to the fields of wearable equipment, transdermal drug delivery and biological signal processing. The bracelet is integrated with a multi-mode biosensor which comprises a temperature sensor, a pressure sensor, a heart rate sensor, a blood oxygen sensor and a skin electric response sensor, and physiological data related to pain can be collected in real time. Through an embedded microcontroller and a machine learning algorithm, a pain intensity evaluation model is constructed, and real-time evaluation of the pain state of a patient is realized. Based on the pain intensity and the physiological state of a patient, a drug release strategy is dynamically adjusted through an intelligent algorithm, and drugs are released painlessly and accurately through a micro-needle transdermal drug delivery technology, so that the patient obtains the optimal treatment effect. And the functions of data storage, historical analysis and remote doctor monitoring can be expanded. The hand ring is convenient to operate, comfortable to use and suitable for various pain management scenes such as chronic pain and postoperative pain, and the personalized level of pain treatment and patient compliance are remarkably improved.
Owner:THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
Vibrio parahaemolyticus deoxyribozyme label-free biosensor
PendingCN120330224AHydrolasesMicrobiological testing/measurementVibrio parahaemolyticusBioinformatics
The invention discloses vibrio parahaemolyticus deoxyribozyme screening and label-free biosensor development. The vibrio parahaemolyticus deoxyribozyme screening and label-free biosensor development comprises the following steps: (1) obtaining a vibrio parahaemolyticus deoxyribozyme sequence; (2) optimizing reaction conditions of the vibrio parahaemolyticus label-free biosensor; and (3) detecting the vibrio parahaemolyticus. The detection principle of the sensor is as follows: when vibrio parahaemolyticus exists, deoxyribozyme is triggered to be self-cleaved, a generated cleaved short chain can be used as a primer for starting rolling circle amplification (RCA), a large number of long-chain DNA products with repeated series G-quadruplex sequences are generated by amplifying a designed RCA annular template, G-quadruplex and ThT can form a G-quadruplex / ThT compound, and the long-chain DNA products can be used for detecting the vibrio parahaemolyticus. The fluorescence is generated under the excitation wavelength of 439 nm, and the higher the concentration of the vibrio parahaemolyticus is, the higher the fluorescence intensity of the system is, so that the simple, convenient, rapid, low-cost and high-sensitivity detection of the vibrio parahaemolyticus is realized.
Owner:CHINA AGRI UNIV +1
Intelligent early warning system and bracelet for Parkinson / epilepsy recognition based on multi-modal sensor
The invention discloses an intelligent pre-warning system for Parkinson / epilepsy recognition based on a multi-modal sensor and a bracelet, and relates to the technical field of biosensors. The intelligent early warning system for Parkinson / epilepsy recognition based on the multi-modal sensor comprises a multi-modal acquisition interference judgment module, a multi-modal interference processing optimization module and a multi-modal fusion input early warning module. According to the method, whether the multi-modal interference processing optimization is carried out or not is judged according to the multi-modal interference early warning result, if yes, the multi-modal fusion instruction is sent after the multi-modal interference processing optimization, and if not, multi-modal biological data fusion is directly executed, and the multi-modal fusion early warning result is obtained. Finally, whether multi-modal fusion input early warning is carried out or not is judged based on the multi-modal fusion early warning result, the effect of carrying out physical condition early warning more accurately is achieved, and the problem that in the prior art, in the process of carrying out physical condition early warning through wearable equipment, multi-modal biological data collection and fusion association are not sufficient is solved.
Owner:JIANGSU JINLING ZHISHU MEDICAL TECHNOLOGY CO LTD
Paper microfluidic wearable screen printed biosensor for sweat analysis
An apparatus, system, and method for providing a moisture supplementation detection device. The moisture replenishment detection device includes: a flexible substrate having a circuit screen-printed therein, the circuit including a plurality of conductive ink layers and a dielectric ink layer; a sensor on the flexible substrate, wherein the sensor is capable of detecting element data indicative of a characteristic of sweat; an untreated chromatographic paper placed in contact with the skin of the user and capable of wicking sweat from the skin to the sensor along the micro-sized fluid channel; and a Bluetooth antenna on the flexible substrate and in communication with the sensor, and capable of wirelessly communicating the element data to an application capable of indicating to the user the user the suitability of moisture replenishment based on the element data.
Owner:JABIL INC
Construction and application of confinement-enhanced photoelectrochemistry-electrochemiluminescence dual-mode biosensor
PendingCN121454049AMaterial nanotechnologyTransportation and packagingPeroxidaseElectrochemiluminescence
The invention discloses construction and application of a confinement enhanced photoelectrochemistry-electrochemiluminescence dual-mode biosensor, and relates to the technical field of biosensing and analytical chemistry. The preparation method of the biosensor comprises the following steps: taking a CdS (at) SiO2 (at) NaYF4: Yb / Tm nano composite material as a substrate to obtain a confinement enhanced photoelectrochemistry (PEC) and electrochemiluminescence (ECL) sensing interface; in combination with a DNA wheel nanostructure triggered by target acetamiprid, signal amplification is realized; and synchronous dual-mode quenching detection of PEC and ECL signals is realized by virtue of peroxidase-like catalytic precipitation and wide-spectrum absorption characteristics of the PtPd-CoSnO3 nanocube. The biosensor prepared by the invention has the advantages of high sensitivity, strong reliability, good selectivity and the like, can be applied to accurate detection of acetamiprid in samples such as agricultural products and the like, and has important application value in the aspects of environmental monitoring and food safety control.
Owner:XUZHOU NORMAL UNIVERSITY
A gene chip sensor for single nucleotide polymorphism detection and its application
A gene chip sensor for single nucleotide polymorphism detection and its application belong to the field of biosensor detection technology. In order to solve the technical problems of low specificity and accuracy, long processing time and high cost in the existing single nucleotide polymorphism detection methods, the present invention provides a gene chip sensor for single nucleotide polymorphism detection, wherein the gene chip sensor comprises WT wild-type and SNP mutant nucleotide sequences, LSL probe, and CRISPR / Cas 12a system. LSL probe is used as a recognition intermediate to activate the CRISPR / Cas12a system, iron-cobalt nanomaterials are used to provide a stable signal source for sample detection, and screen-printed electrodes are combined with electrochemical sensors; the application of CRISPR / Cas12a and nanomaterial technology in molecular breeding is expanded, and technical support is provided for economical and efficient SNP detection.
Owner:CHANGCHUN DONGYI YUXIN BIOTECHNOLOGY CO LTD
DNAzyme-based gene chip biosensor and application thereof
ActiveCN120310956BMicrobiological testing/measurementMaterial electrochemical variablesNucleotideGenotyping
The application relates to a DNAzyme-based gene chip biosensor and application thereof, and belongs to the technical field of electrochemical sensors. In order to solve the technical problems of low specificity and accuracy, long processing time and high cost in the single nucleotide polymorphism detection method in the prior art, a DNAzyme-based gene chip biosensor is provided, which comprises a DNAzyme molecular probe SNP recognition system and a solid-liquid phase mixed catalytic network system. The application provides a proteinase-free and space-oriented biosensing strategy, utilizes a molecular switch based on a DNAzyme probe to realize accurate SNP recognition, utilizes a space steric hindrance parallel amplification system to enhance signal amplification and simultaneously inhibit background noise, so that the detection performance is remarkably improved; and the gene chip biosensor can be applied to genotyping and trait selection in crop improvement and other applications.
Owner:CHANGCHUN DONGYI YUXIN BIOTECHNOLOGY CO LTD
Foreign language learning and homework doing system and method based on education artificial intelligence and integrating interest, real scene and like
The invention discloses a foreign language learning and homework doing system and a foreign language learning and homework doing method integrating education artificial intelligence, augmented reality (AR) technology and biological signal perception. A multi-mode biosensor (electrodermal response, heart rate, eye movement and the like) is integrated through customized AR intelligent glasses, and a closed loop of'interest scene triggering-biological signal sensing-personalized content pushing-self-adaptive review planning based on a forgetting curve and real-time emotion data 'is realized in combination with an interest matching algorithm and a dynamic learning engine of an intelligent foreign language learning APP. The system utilizes an artificial intelligence technology to accurately analyze the emotional state of a user, adopts a bidirectional long short-term memory network (Bi-LSTM) personalized forgetting curve algorithm, integrates foreign language words, grammar and other homework contents into an augmented reality or virtual interest scene, and supports on-school students to complete homework tasks and off-school users to autonomously learn. The system covers hardware architecture design, AI algorithm implementation and a data interaction process, foreign language learning and homework doing are completed during playing, the learning efficiency is remarkably improved, and the system has high innovativeness and practicability.
Owner:张景飞
Electrochemical biosensor of light-driven nanopore channel and application of electrochemical biosensor
The invention discloses an electrochemical biosensor of a light-driven nanopore channel and application of the electrochemical biosensor. According to the sensor, a photoresponse nanopore membrane is constructed, an outer surface aptamer probe is combined, asymmetric photothermal gradient driven ion transmission induced by near-infrared light is utilized, and ultra-sensitive detection on a target object is realized by regulating and controlling the charge density of the outer surface. According to the electrochemical biosensor provided by the invention, traditional voltage driving is replaced by asymmetric near-infrared light driving, so that the electrochemical biosensor has a relatively wide detection range and an extremely low detection limit, and high-sensitivity and specific detection on a target object can be realized. The electrochemical biosensor can be used for detecting microcystin-LR (MC-LR), the detection limit reaches 1 * 10 <-7 > mu g / L, the sensitivity is improved by 5 orders of magnitude compared with that of a traditional voltage-driven sensor, and the electrochemical biosensor can be applied to trace pollutant detection in the fields of environmental monitoring, food safety and the like.
Owner:CHINA UNIV OF GEOSCIENCES (WUHAN)
Method for high-throughput screening of key genes in Escherichia coli 1, 5-pentamethylene diamine biosynthetic pathway
The invention discloses a method for high-throughput screening of key genes in a 1, 5-pentamethylene diamine biosynthetic pathway of escherichia coli, and the high-throughput screening is carried out by using a 1, 5-pentamethylene diamine biosensor. The 1, 5-pentamethylene diamine biosensor is a recombinant plasmid obtained by integrating a lysine decarboxylase coding gene cadA and a promoter trc thereof, and a green fluorescent protein (GFP) coding gene and a promoter pyrF thereof into a plasmid skeleton. A fluorescent quantitative flow cytometer is used for screening single-cell thalli with high yield of 1, 5-pentanediamine according to the fluorescence intensity of GFP, key genes can be rapidly screened out from a large number of genes to be screened in a high-throughput mode, and the screening workload and time are greatly saved. According to the application of the key gene in the biosynthetic pathway of the escherichia coli 1, 5-pentanediamine screened by the method disclosed by the invention, the yield of the 1, 5-pentanediamine produced by fermenting the escherichia coli KA30 can be greatly improved, the substrate conversion rate in the fermentation process is improved, the production cost of the 1, 5-pentanediamine is reduced, and the economic benefit is improved.
Owner:NANJING TECH UNIV
Nucleic acid aptamer specifically combined with MMLV as well as preparation method and application of nucleic acid aptamer
The invention belongs to the technical field of bioengineering, and particularly relates to a nucleic acid aptamer specifically bound with MMLV, and the nucleotide sequence of the nucleic acid aptamer is as shown in SEQ ID NO.1 or SEQ ID NO.2; the nucleic acid aptamer capable of being specifically bound with MMLV is obtained through screening on the basis of the SELEX technology, has high affinity for MMLV protein, can be applied to MMLV protein enrichment reagents, MMLV protein separation reagents, MMLV protein detection reagents, test paper and biosensors, and facilitates identification and activity research of the MMLV protein.
Owner:THE UNIVERSITY-TOWN HOSPITAL AFFILIATED TO CHONGQING MEDICAL UNIVERSITY
Method for detecting 17 beta-estradiol by using colorimetric-fluorescence dual-mode biosensor
PendingCN120064263AMaterial analysis by observing effect on chemical indicatorBiological material analysisAptamerOxidative enzyme
The invention discloses a method for detecting 17 beta-estradiol by using a colorimetric-fluorescent dual-mode biosensor, and belongs to the technical field of chemical analysis and detection. According to the method, a colorimetric-fluorescent dual-mode biosensor for detecting 17 beta-estradiol is established based on oxidase-like activity and fluorescence quenching characteristics of manganese dioxide nanosheets and catalytic characteristics of alkaline phosphatase by taking a nucleic acid aptamer as a recognition molecule. The synthesis cost of the nucleic acid aptamer is low, the method has universality, and other target objects can be detected by changing the nucleic acid aptamer; mBs-DNA-ALP is prepared by using a biotin-streptavidin reaction and a DNA hybridization reaction, and a structure conversion process from double-stranded DNA to an aptamer / target compound is realized by using targeted induction, so that a detection signal is amplified; according to the method for detecting 17 beta-estradiol through colorimetric-fluorescence signals, dual-mode output is achieved, results are verified and supplemented mutually, and the specificity and accuracy of the method are improved.
Owner:JILIN UNIVERSITY
Blood concentration detection method
The invention discloses a plasma concentration detection method, and belongs to the field of biomedical detection.The plasma concentration detection method comprises the steps that a saliva sample and a mesenchymal fluid sample of a to-be-detected object are synchronously collected through non-invasive equipment and minimally invasive equipment, and after centrifugation and membrane filtration pretreatment, the saliva sample and the mesenchymal fluid sample are obtained; a multi-channel biosensor is used for detecting drug metabolite concentrations in saliva and interstitial fluid respectively, a blood drug concentration predicted value is further calculated through a composite calibration model, the model is constructed based on a random forest algorithm, saliva and interstitial fluid concentration data are input, the blood drug concentration is output, and dynamic correction is conducted through species characteristic parameters. Wherein species parameters are obtained in real time through near infrared spectrum, weight coefficients (alpha and beta) are optimized in combination with a multiple regression equation, and finally high-precision prediction is achieved through a weighting algorithm. The method has the advantages of non-invasiveness, cross-species universality, dynamic adaptive correction and the like, and is suitable for clinical monitoring, veterinary medicine and pharmacokinetic research.
Owner:SUZHOU LEO BIOTECH
Sensor integrating sweat induction and multi-parameter detection as well as preparation method and application of sensor
The invention discloses a sweat induction and multi-parameter detection integrated sensor and a preparation method and application thereof, and belongs to the technical field of flexible biosensing, a PDMS flexible substrate is adopted to construct a multi-layer integrated structure, the sweat induction and multi-parameter detection integrated sensor comprises a sweat guide layer, a sweat collection layer, a detection electrode layer and a micro-fluidic chip layer, sweat secretion is promoted by accurately controlling the temperature to be less than or equal to 40 DEG C; the detection electrode layer adopts a silk-screen printing process to prepare a three-electrode system, comprises a specifically modified glucose working electrode and a beta-hydroxybutyric acid working electrode and can synchronously detect the concentration of glucose and beta-hydroxybutyric acid in sweat, and the micro-fluidic chip layer is integrated with a Bluetooth transmission module to realize real-time wireless transmission of detection data to a mobile terminal; the biosensor has the advantages of being good in biocompatibility, high in detection sensitivity and comfortable to wear and can tolerate bending with the curvature radius larger than 5 mm, and a non-invasive and continuous metabolism monitoring solution is provided for diabetes management.
Owner:SUZHOU UNIV OF SCI & TECH
Terahertz biosensor based on asymmetric hollow cylinder metasurface and method thereof
The invention discloses a terahertz biosensor based on an asymmetric hollow cylinder metasurface and a method thereof. The sensor is obtained by periodically arranging metasurface units; each metasurface unit is composed of a dielectric substrate and a hollow cylinder array unit. According to the invention, asymmetric disturbance is introduced to asymmetric arrangement of four hollow cylinders of the hollow cylinder array unit, terahertz waves are induced to form a quasi-continuum bound state (Q-BIC) harmonic peak with an ultrahigh Q value on the hollow cylinder array unit, and the Q value up to 20000 or above is realized by using a quasi-continuum bound state induction mechanism. An obvious response signal can be generated in a terahertz wave spectrum, so that ultra-sensitive detection on trace and even trace biological substances can be realized, and the detection efficiency and accuracy are greatly improved.
Owner:CHINA JILIANG UNIV