44 results about "Creatinine" patented technology

There is provided a method for diagnosing a subject with early onset muscle atrophy, said method comprising: obtaining a biosample from the subject; and assaying the biosample for one or more biomarkers, said one more biomarkers are taurine, proline, citrulline, trigonelline, thymidine, ornithine, glutamate, L-pyroglutamic acid, creatinine, adenine, nicotinamide, 2-methylhippuric acid, maltol, L-arginine, hypotaurine, L-glutamine, homogentisic acid, methylhistidine, oxoglutaric acid, xanthine, L-carnitine, succinate, or a combination thereof; and identifying the subject with early onset muscle atrophy on the basis of a deviation in the one or more of said one more biomarkers.

The invention provides an organoid basic culture medium and a method for maintaining and promoting organoid growth, and the organoid basic culture medium comprises a basic culture medium and an additive, the additives comprise taurine sodium 3-hydroxybutyrate, taurine, sodium hypoxanthate, creatinine, L-ornithine, urea, L-sodium lactate, putrescine dihydrochloride, anhydrous citric acid, ethanolamine, N-acetylglycine, alpha ketoglutaric acid, formic acid, malic acid, malonic acid, succinic acid, uric acid, ammonium metavanadate, manganese dichloride, sodium selenite, creatine, glycerol and uridine. Anhydrous sodium acetate and para aminobenzoic acid. When the organ-like basic culture medium is used for treating the organ-like, the activity of the organ-like can be effectively maintained, and basic nutrition and metabolism requirements of the organ-like can be provided, so that the organ-like has the advantages of high activity, fast proliferation and long-term stable passage, and is suitable for various types of organs and wide in application.

The invention provides a diagnostic marker for metabolic syndrome and preclinical stage thereof, and the diagnostic marker or a plasma small molecule metabolism biomarker consists of the following substances: pyrimidine, (S)-beta-aminoisobutyric acid, 3-hydroxybutyric acid, L-serine, guanidine acetic acid, 3-hydroxyisovaleric acid, L-homoserine, L-cysteine, piperidine acid, hypotaurine and creatinine. The beverage is prepared from phenylacetic acid, erythritol, picolinic acid, taurine, pyroglutamic acid, lemonic acid, malic acid, tyramine, isonicotinic acid, o-phosphoethanolamine, L-glutamine, salicylic acid and D-glucose. The invention also provides a kit for detecting the plasma small molecule metabolism biomarker composition, the spectrum peak intensity of the obtained plasma small molecule metabolism biomarker is detected, the risk score of whether each subject is MetS or pre-MetS is calculated, and then whether the subject is MetS or pre-MetS is predicted. According to the invention, efficient and rapid diagnosis of the metabolic syndrome and the preclinical stage thereof is realized.

The invention discloses a protein / polysaccharide composite dialysis membrane based on in-situ loaded lanthanum carbonate and application of the protein / polysaccharide composite dialysis membrane, a lanthanum carbonate loaded protein / polysaccharide composite aggregate is used for modifying a porous filter membrane, and the dialysis membrane with a lanthanum carbonate loaded protein / polysaccharide self-assembled membrane as a compact skin layer and the porous filter membrane as a supporting layer is formed. By integrating a membrane separation mechanism of hemodialysis and an adsorption principle of blood perfusion, the skin layer of the dialysis membrane has molecular sieve separation and chemical adsorption functions, efficient removal of molecular uremic toxins (phosphorus, creatine liver, bilirubin, p-cresol sulfate and indol sulfate) with different sizes is synergistically realized, macromolecular proteins in blood are effectively intercepted by pore size screening, and the dialysis membrane has the advantages of simple structure, low cost and high efficiency. According to the present invention, by using the adsorption effect of the functional group on the surface of the membrane, the high removal rate on the protein-bound urotoxin is achieved, the high removal effect on phosphorus is particularly achieved, the blood compatibility is considered, the large-scale preparation is easily achieved, and the new idea is provided for the development of the high-performance blood purification material.

The invention discloses a method for constructing a MeIQx generation simulation system, which comprises the following steps: S1, constructing a system: adding diethylene glycol and water into a reaction tube, adding a certain amount of glycine, creatinine and glucose into the reaction tube, mixing to obtain the MeIQx generation simulation system, and uniformly mixing; s2, construction of different systems: preparing two parts of MeIQx generation simulation systems, adding soybean oil into one part of the MeIQx generation simulation systems to obtain an oil-containing MeIQx generation simulation system, and not adding the other part of the MeIQx generation simulation system to obtain an oil-free MeIQx generation simulation system; s3, adding an inhibitor: respectively adding a certain amount of inhibitor into the two systems, and uniformly mixing; s4, reaction simulation: heating the reaction tubes of the two systems at a certain temperature, and cooling after heating; and S5, substance detection: generating simulation system solutions by the two MeIQx after reaction simulation, and respectively detecting the content of the MeIQx, a precursor substance and an intermediate substance. By constructing a chemical simulation system and adding an exogenous inhibitor, a scientific basis is provided for accurate reduction and control of MeIQx in the high-temperature processing process of aquatic products.

The present invention provides a method for introducing nucleic acids, proteins, or complexes thereof into cells with excellent efficiency, in particular, a method using a transduction solution. The method for transducing a molecule of interest into a cell of the present invention comprises the step of contacting the cell with the molecule of interest and a transduction solution, wherein the transduction solution comprises at least one of the following (A1) to (A5) and (B) a salt: (A1) a compound represented by the following general formula (I) or a salt thereof, but excluding a specified compound; (A2) a compound represented by the following general formula (II) or a salt thereof; (A3) a nucleic acid base or a salt thereof; (A4) a compound represented by the general formula (III) or a salt thereof, but excluding malic acid; (A5) at least one selected from the group consisting of creatinine, hydroxyproline, 1,3-butanediol, trientine, D-cellobiose, 1,3-dimethylurea, pantothenic acid, and trimethyldione, or a salt thereof. #imgabs0#

The application provides a method for improving the flavor and quality of tilapia surimi products, and belongs to the technical field of aquatic product processing, and comprises the following steps: tilapia muscles are cut into small pieces, an improving agent is added, and the muscles are ground; the well-stirred surimi is made into fish cakes and shaped; the fish cakes are taken out after floating, and are placed in cold water for cooling; the shaped fish cakes are respectively fried at 150 DEG C, 180 DEG C and 210 DEG C for 4 min; the fish cakes are vacuum packaged and stored in a refrigerator after cooling; the flavor of the surimi is analyzed, the quality of the surimi is detected, and the deterioration of the surimi is determined and analyzed; threonine, creatinine and glucose are dissolved in a phosphate buffer to obtain A liquid; diethylene glycol is taken, mixed with soybean oil, and mixed with an improving agent by vortexing to obtain B liquid; the A liquid and the B liquid are fully mixed in a 48 mL sealed high-temperature pressure-resistant reaction bottle, and then are placed in an oil bath for reaction and then are placed in an ice water bath for reaction termination; the solution after reaction is removed and is reserved; the MeIQx content, precursor substances and intermediate substances of the MeIQx generation simulation system solution after reaction simulation are determined.

A method for preparing glutaryl-bridged bis-biogenic guanidine chelate (GbG)MX2 including contacting biogenic guanidine (G) with glutaryl chloride in DMSO solvent for an acylation reaction, to yield glutaryl-bridged bis-biogenic guanidine GbG (G-b-G), and mixing the glutaryl-bridged bis-biogenic guanidine GbG as a chelating ligand and a non-toxic metal salt MX2 in an amphiphilic mixed solvent DMSO-H2O for a ligand addition reaction, to yield glutaryl-bridged bis-biogenic guanidine chelate (GbG)MX2. The biogenic guanidine (G) is selected from arginine (Arg), guanidine acetic acid (Gaa), creatine (Cra), and creatinine (Cran). M represents Fe2+, Mg2+, or Zn2+; and X represents CI−, AcO−(CH3COO−), LaO−(CH3CH(OH)COO−). The glutaryl-bridged bis-biogenic guanidine chelate (GbG)MX2 can be used as a catalyst for production of polybutylene succinate (PBS) using succinic anhydride (SAn) and BDO as monomers through a batch process or a continuous process. The catalyst of (GbG)MX2 of the disclosure is non-toxic, and the PBS synthesized features excellent environmental and biological safety.

The present disclosure relates to biocide compositions compatible with enzyme biosensors and methods of use thereof. More particularly, the present disclosure relates to biocide compositions compatible with enzymes used to measure creatine and creatinine levels.

The invention relates to application of creatinine in cosmetics and related products, and belongs to the technical field of cosmetics. According to the scheme, the creatinine with relieving, anti-inflammatory and whitening effects is added and matched with the nicotinamide to be used in the cosmetic composition, the adding amount of the nicotinamide is 1.0-4.0 wt%, the adding amount of the creatinine is 0.0001-2.0 wt%, the creatinine and the nicotinamide are matched, the equivalent and even slightly good whitening effect can be achieved, meanwhile, the using amount of the nicotinamide is effectively reduced, and the skin whitening effect is improved. Therefore, the problems of skin allergy, redness, swelling, acnes and other side effects caused by addition of high-concentration nicotinamide in the prior art are solved, in addition, the essence lotion product provided by the invention also has the effects of moisturizing, repairing and maintaining a weak acid skin environment, can meet the requirements of consumers for moisturizing, whitening and maintaining skin health, and has a good market prospect. And the method has important market economic value.

The present invention relates to a method by which a server detects renal disease of a companion animal on the basis of protein, creatinine and specific gravity, and is a technique related to a method for detecting renal disease of a companion animal on the basis of protein, creatinine and specific gravity, the method comprising the steps of: (a) constructing an RGB ratio table, and photographing a urine test strip stained with urine, so as to generate a test image; (b) generating a first RGB value by extracting, from the test image, RGB values for each of a protein pad area and a creatinine pad area corresponding to a renal test item area from among the test item areas of the urine test strip; and (c) generating a first ratio value through the generated first RGB value and a specific gravity RGB value extracted from a specific gravity pad area in the test image, generating a second ratio value, which is the ratio between the specific gravity of the RGB ratio table, and protein and creatinine, correcting the RGB value of the protein pad area and the RGB value of the creatinine pad area by comparing the first ratio value and the second ratio value, and determining the renal health state of the companion animal by comparing the corrected RGB values.

The present invention provides a method of assessing the risk of an individual to suffer from epithelial cancer, an analyzer therefor, and a kit thereof, the method comprising: measuring the content of a metabolite in a sample of the individual, the metabolite comprising urea nitrogen, creatinine, or a combination thereof; and comparing the contents of the metabolites of the negative control group and the sample, when the content of the metabolites of the sample is lower than the content of the metabolites of the negative control group, the individual is assessed to have the risk of suffering from the urinary epithelial cancer, and the kit comprises at least one reagent for identifying the metabolites in the sample of the individual so as to accurately assess the risk.

The application discloses a kind of growing and fattening pig compound feed and its preparation method and application.The compound feed includes sesame stem leaf and basic daily ration, and the sesame stem leaf accounts for 2%~40% of the mass of compound feed.The application also discloses the preparation method of the compound feed, the application of sesame stem leaf in the preparation of the compound feed and the method for feeding growing and fattening pig.The compound feed of the application has no significant influence on the growth performance of growing and fattening pig after adding sesame stem leaf, has no safety hidden trouble to the growth process and edible part of pig, can improve pork color brightness, reduce back fat thickness, improve lean meat rate, improve pork tenderness, significantly reduce serum alanine aminotransferase, total bile acid and creatinine levels, improve liver and kidney function, and has no histopathology influence on main organs, with high safety.The application widens the feed application field of sesame as high-protein plant resources, and can be used to replace part of soybean meal, to alleviate the problem of high dependence on external feed raw materials.

The invention provides application of parabacteroides dieldrii in preparation of a medicine for treating sepsis, and belongs to the technical field of microorganisms. According to the application disclosed by the invention, the sepsis is treated by utilizing the parabiosis dielsii ATCC: 8503, the treatment effect of the parabiosis dielsii ATCC: 8503 is detected, and a result proves that after the parabiosis dielsii ATCC: 8503 is given, the survival rate of mice with sepsis can be obviously increased, the contents of ALT, AST, BUN and Creatinine in serum can be reduced, meanwhile, the wet / dry weight ratio of lung is reduced, and the liver, lung and kidney injury caused by sepsis is effectively relieved.

The utility model provides a semipermeable membrane for hemodialysis and a hemodialysis device. Specifically, the semipermeable membrane sequentially comprises a first polyester fiber non-woven fabric supporting layer and a second polyester fiber non-woven fabric supporting layer, a first chitosan layer; a porous polysulfone membrane, wherein the average pore size of pores in the porous polysulfone membrane is in the range of 5 nm to 50 nm; a second chitosan layer; and a second polyester fiber non-woven fabric supporting layer. The semi-permeable membrane for hemodialysis has good biocompatibility, wider waste removal capacity (besides small molecular substances (such as urea and creatinine), middle molecular substances (such as beta2-microglobulin) can be effectively removed) and good durability.

The invention provides a paper-based sensor for creatinine detection and a preparation method thereof, the paper-based sensor comprises a polylactic acid paper-based material and a colorimetric indicator attached to the polylactic acid paper-based material, and the colorimetric indicator is formed by curing water-based colorimetric indication ink; the water-based colorimetric indication ink comprises a solvent, an indicator, a stabilizer and a thickening agent, the indicator is a meta-dinitro aromatic compound, the stabilizer is a water-soluble inorganic alkaline compound, and the thickening agent is a biomass polysaccharide derivative. The paper-based sensor provided by the invention can be used for portable handheld detection of creatinine in a solution, the level of creatinine is judged through comparison with a colorimetric card, compared with a mainstream enzyme method in the market, the response speed is higher, the cost is lower, and the polylactic acid paper base material and the colorimetric indicator can be naturally degraded, so that the paper-based sensor is more environment-friendly.

The invention relates to the technical field of treatment of pollutants in wastewater, in particular to an anti-interference silver-vanadium metal-organic framework derivative material as well as a preparation method and application thereof. The preparation method comprises the steps that firstly, an AgV-MOF material with a two-dimensional twisted wave layered structure is prepared, then an Ag / V-MOF derivative material is prepared through alkaline etching, the Ag / V-MOF derivative material is provided with the wrinkled two-dimensional twisted wave layered structure, a large number of-OH groups are attached to the surface, and the Ag / V-MOF derivative material is prepared. Silver ions and vanadium ions can form an electron transfer path through bridging of organic ligands, so that electron transmission and transfer in the material are facilitated, and the adsorption performance of active sites of the material is further improved; therefore, the Ag / V-MOF derivative material can present the characteristics of high efficiency, short time and strong anti-interference performance when adsorbing creatinine under the combined action of the charge on the surface of the material, the conjugated structure of the ligand, electron transfer of the metal center and the two-dimensional twisted wave layered structure with the folds.

The present invention relates to methods for detecting and / or measuring alanine transaminase (ALT) activity, aspartate transaminase (AST) activity, alkaline phosphatase (ALP) activity, glucose levels, creatinine levels, urea levels, asymmetric dimethylarginine (ADMA) levels, and / or symmetrical dimethylarginine (SDMA) levels in a sample. The method allows all of the activities and analytes, or any combination of activities and analytes, to be measured in a single assay.

Disclosed is a personal care composition comprising carboxymethyl cysteine compound and creatinine compound, wherein the creatinine compound is selected from creatinine, salt of creatinine, solvate of creatinine, hydrate of creatinine and a combination thereof and the carboxymethyl cysteine compound is present in amount of at least 0.00001% and no greater than 10% by weight of the composition.

Creatinine levels may be monitored as a measure of kidney function. Conventionally, blood and / or urine tests are used for this purpose. Analyte sensors capable of monitoring creatinine in vivo may comprise: a sensor tail comprising at least a first working electrode, a creatinine-responsive active area disposed upon a surface of the first working electrode, a first membrane that is permeable to creatinine and overcoats the creatinine-responsive active area, and an oxygen scavenger located upon the sensor tail in proximity to the creatinine-responsive active area. The creatinine-responsive active area comprises a first electron transfer agent, a first polymer, and an enzyme system comprising multiple enzymes, particularly creatinine amidohydrolase, creatine amidohydrolase, and sarcosine oxidase, that are capable of acting in concert to facilitate detection of creatinine. An oxidase enzyme may serve as the oxygen scavenger, particularly glucose oxidase when detecting creatinine in fluids also containing glucose.

The invention discloses application of a semen nigellae extract in preparation of a product for preventing and / or treating androgenetic alopecia. The total content of nigella alkaloid and nigella alkaloid 4-O-sulfite in the nigella seed extract is not less than 2%. The preparation process comprises the following steps: degreasing nigellum nigrum medicinal material powder with petroleum ether, extracting with an acidic aqueous solution, and enriching with macroporous adsorption resin and specifically purifying with anion exchange resin in sequence. Experiments show that the extract can most significantly reduce the levels of testosterone (T), dihydrotestosterone (DHT) and creatinine (Cr) in an androgenetic alopecia model mouse body, increase the level of estradiol (E2) and effectively promote back hair regeneration, and the effect of the extract is significantly superior to that of a water extract, an alcohol extract and a positive control drug. The extract is clear in component and controllable in quality, can be used for preparing medicines or wash supplies with multi-target anti-hair loss and hair growth functions, and provides a novel efficient natural raw material for treating androgenetic alopecia.

This method for assisting the diagnosis of mental stress comprises: a step for measuring the concentrations of a plurality of components contained in a biological sample collected from a subject; classifying the detected persons based on the personality features; and generating stress diagnosis assistance information for diagnosing the degree of mental stress of the subject on the basis of the determined concentrations of the components including betaine, creatine, GABA, creatinine, serotonin, kynurenic acid, 3-hydroxybutyric acid, and citric acid and the classification of the personality characteristics. According to the method, information serving as a support for accurately diagnosing the presence or absence or degree of mental stress of a subject can be provided.

The present invention relates to a disposable test strip for quantitatively determining the concentration of non-sodium cationic electrolytes and the concentration of creatinine and for subsequent determination of their ratio, and to a non-invasive point-of-care (POC) device for detecting electrolyte imbalance in a patient. In addition, the present invention relates to a method for simultaneously quantitatively determining the concentration of cationic electrolytes and the concentration of creatinine in a urine sample of a patient, and to a method for detecting electrolyte imbalance in a patient.

The present disclosure relates to a creatinine sensor comprising a first working electrode, a creatinine sensing layer on the first working electrode, and a hydrophilic polyurethane film covering the creatinine sensing layer, the creatinine sensing layer comprises a redox medium, creatinine acylamino hydrolase, creatinine amidino hydrolase and sarcosine oxidase. The creatinine sensor may further include a background sensing electrode that does not detect creatinine. The present disclosure further relates to a method for sensing creatinine, comprising: exposing a creatinine sensor having a background sensing electrode to a fluid; applying a potential to the first working electrode and the second working electrode; obtaining, from the first working electrode, a first signal proportional to the concentration of creatinine and background interference in the fluid; obtaining a second signal proportional to the concentration of background interference in the fluid from the second working electrode; and determining the concentration of creatinine in the fluid by subtracting the second signal from the first signal.

The invention provides a method of detecting a brain tumour in a subject, the method comprising the steps of: (i) measuring the amount of at least one metabolite in a sample of cerebrospinal fluid (CSF) taken from a subject, wherein the at least one metabolite is selected from the group consisting of: propionyl carnitine, butyryl carnitine, creatine, creatinine, valine, glutamine, leucine, betaine, isoleucine, asparagine, pyruvate, acetyl carnitine, carnitine, proline, valine and any combination thereof; and (ii) analysing the amount of the at least one metabolite in the sample of CSF, wherein when the amount of the at least one metabolite in the sample of CSF is above a threshold amount a brain tumour in the subject is detected. The invention further provides a method of detecting a brain tumour in a subject comprising identifying elevated or reduced amounts of metabolites in the CSF of a subject, compared to concentrations of the same metabolites in subjects known not to have a brain tumour.

The present invention provides a type of TB-thiophene-pyridinium photodynamic bactericidal photosensitizer and its preparation and application. 4-bromoaniline, paraformaldehyde, 5-aldehyde-2-thiopheneboronic acid and iodomethane are selected as raw materials and synthesized through multi-step reactions to obtain: #imgabs0# Three TB-thiophene-pyridinium derivatives have excellent photophysical properties, pH applicable range, viscosity response, AIE properties and good biocompatibility. DCHA probe detects that all three products can produce ROS, and under the same conditions, the ROS yield of 14 is higher. DHR123 probe and EPR detect that all three compounds can produce superoxide anions. The three products have low dark toxicity and phototoxicity to normal cells; they have low dark toxicity but high phototoxicity to A549 (human non-small cell lung cancer cells), and the IC of A549 cells after illumination is low. 50 The serum creatinine levels were significantly reduced from >100 to 0.31, 0.11, and 1.4 μg·mL, respectively. ‑1 , and has a high safety factor, making it an excellent PDT anti-tumor photosensitizer with great value for further research and development.

An exemplary embodiment of the present disclosure provides a biosensor that can facilitate detection of the molecules creatinine, creatine and / or sarcosine in a minimal-equipment, portable, and low-cost fashion. Some aspects of the disclosure relate to methods and systems to enable quantitative measurement of certain biomarkers at the point of care without expensive instrumentation.

Systems and methods for determining an alarm status for a health index using the health index and one or more pieces of chemical information are disclosed. The chemical information may include potassium levels, creatinine levels, etc. The determined alert status may be provided to a user or process.

The invention belongs to the technical field of biology, and particularly relates to application of bacteroides vulgaris in preparation of medicine for preventing and treating sepsis injury, the sepsis injury is kidney injury caused by sepsis, the survival time of mice with sepsis can be obviously prolonged after pretreatment of bacteroides vulgaris, the survival rate of the mice is increased, and the application of bacteroides vulgaris in preparation of medicine for preventing and treating sepsis injury is achieved. In addition, the traditional Chinese medicine composition can obviously improve mouse kidney tissue morphological lesions, inhibit CLP-induced mouse kidney tissue pathological morphological injuries, obviously reduce serum BUN and Creatinine contents and slow down mouse CLP-induced sepsis kidney injuries, and a new medicine is provided for preventing and treating sepsis injuries.

Systems, devices, and methods include approaches involving comparing an analyte level measured using a chemical sensor and based on an optical property to a threshold, determining that a creatinine level, a potassium level, or a sodium has reached the threshold, and generating a visual recommendation on a user interface to consume a source of protein, a source of potassium, or a source of sodium.