203 results about "Enzyme system" patented technology

Glucose and ketones may be dysregulated singularly or concurrently in certain physiological conditions and may be advantageously assayed together using an analyte sensor capable of detecting both analytes. Certain analyte sensors capable of dual detection may comprise a first working electrode and a second working electrode, a ketones-responsive active area disposed upon a surface of the first working electrode, a glucose-responsive active area comprising a glucose-responsive enzyme disposed upon a surface of the second working electrode, a membrane having a first portion overcoating the ketones-responsive active area and a second portion overcoating the glucose-responsive active area, in which the first portion and the second portion have different compositions. The ketones-responsive active area comprises an enzyme system comprising at least two enzymes that are capable of acting in concert to facilitate detection of ketones.

The invention provides a cobalt monatomic nano-enzyme system modified by phospholipid polyethylene glycol carboxyl. The cobalt monatomic nano-enzyme system comprises a cobalt monatomic nano-enzyme composite core and phospholipid polyethylene glycol carboxyl compounded on the surface of cobalt monatomic nano-enzyme, the cobalt monatomic nano-enzyme composite core comprises a cobalt monatomic nano-enzyme and ginsenoside loaded on the cobalt monatomic nano-enzyme. The invention also provides a bifidobacterium synergetic cobalt monatomic nano-enzyme system which is formed by combining cobalt monatomic nano-enzyme with ginsenoside Rb1 and further delivering the cobalt monatomic nano-enzyme and ginsenoside Rb1 through bifidobacterium bifidum for targeted therapy of enteritis. The combination of Co SA and Rb1 can enhance the anti-inflammatory efficacy by adjusting a key immune signal pathway, and bifidobacterium bifidum can ensure targeted delivery of microbiota and promote intestinal barrier repair. The synergistic three-component strategy integrating monatomic nano-enzyme antioxidation, natural drug immunoregulation and probiotic targeting is provided, and a promising treatment platform is provided for accurate intervention of IBD.

The invention discloses a fresh plant directed biotransformation method based on endogenous enzyme activation and a product and application thereof. The core of the preparation method is as follows: fresh plants (tuberous roots, stems or fruits) which are harvested in a ripe state and have metabolism in a dormant state (the respiration rate is lower than 5 mg CO / kg.h) are taken as raw materials, the raw materials are treated in an environment with the specific pressure being 0.02-0.20 MPa, the temperature being 70-121 DEG C and the humidity being 50-95%, a specific impregnating compound can be selectively combined, and the raw materials can be prepared into the plant culture medium. Under the conditions that exogenous microorganisms are not introduced and plant tissue structures are not damaged, the catalytic function of an endogenous metabolic enzyme system is safely and efficiently restarted and maximally promoted, and components of a plant body are driven to realize directional biotransformation. According to the process, conversion of primary metabolites and / or secondary metabolites is remarkably promoted, the content conversion of one or more beneficial components in the primary metabolites and / or the secondary metabolites is improved, and meanwhile, the biological and pharmaceutical structures and inherent metabonomics characteristics of the plants are completely reserved. The biotransformation product can be used as a high-quality raw material to be applied to medicines, common foods, health foods, special diet foods, daily chemical products and disinfection products, and can be further prepared into traditional Chinese medicine decoction pieces, solid preparations, semi-solid preparations and liquid preparations by adding or not adding pharmaceutically acceptable auxiliary materials.

The invention discloses nano-enzyme coating recombinant probiotics as well as a preparation method and application thereof, and belongs to the technical field of biotechnology and nano medicine. According to the invention, engineered Escherichia coli Nissle 1917 (ECN (DE3)) is taken as a host, and glutathione peroxidase (GPX) and glutathione reductase (GR) genes are over-expressed through a genetic engineering means, so that an efficient antioxidant enzyme system is constructed. And sequentially modifying chitosan and cerium dioxide nano-enzyme (CNP) on the surfaces of the thalli by using an electrostatic layer-by-layer self-assembly technology to form a protective coating. The nano-enzyme coating significantly improves the survival rate of the recombinant probiotics in severe environments such as simulated gastric acid (pH 2.5) and bile salt (0.3%), and enhances the ability of the recombinant probiotics to remove reactive oxygen species (ROS), including hydrogen peroxide, superoxide anions, hydroxyl free radicals and DPPH free radicals.

The invention discloses a co-fermentation method based on sphingomonas and a rhodiola rosea extracting solution and application of a product, and belongs to the technical field of microorganisms. By utilizing a special rich enzyme system of sphingomonas and combining with an early-stage composite enzymolysis process and synergistic effect, the wall breaking efficiency of rhodiola rosea cell walls and the conversion degree of active components of rhodiola rosea are greatly improved, the bottlenecks of low efficiency and low bioavailability of a traditional rhodiola rosea extraction method are successfully overcome, and the rhodiola rosea extraction method is suitable for industrial production. The functional components such as salidroside and flavone can be fully released and converted. Secondly, different from non-symbiotic bacteria (such as lactic acid bacteria and saccharomycetes) which are commonly used for fermentation in the prior art, a potential symbiotic bacterium of sphingomonas XJL-TP07 from a special environment is innovatively selected and has better metabolic suitability with the rhodiola rosea component, the fermentation process is milder and safer (no irritant is produced), and the rhodiola rosea fermentation method is suitable for large-scale production. And a synergistic interaction effect can be generated through a unique biotransformation path.

The application belongs to the technical field of polymer material recycling, and particularly relates to a high-efficiency enzymatic hydrolysis process of polycaprolactam. The high-efficiency enzymatic hydrolysis process of polycaprolactam provided by the application comprises the steps of acid pretreatment, enzymatic catalytic depolymerization and product post-treatment. The process combines sulfuric acid and a multi-enzyme system to treat PA6 with high substrate concentration. The physical form and chemical accessibility of PA6 are significantly changed through sulfuric acid pretreatment, which creates conditions for subsequent enzymatic hydrolysis reaction, and realizes efficient, deep and green degradation of PA6.

In the present application, by knocking out the BG gene in Trichoderma reesei PX3 (a strain of cellulase transcription activator that can be activated continuously), the BG activity can be reduced under the condition of not reducing or even increasing the filter paper enzyme activity of the enzyme system, and a low BG enzyme activity and high filter paper enzyme activity strain is constructed. This strategy can avoid the negative impact on cellulase production after BG knockout, and the obtained strain can be used for efficient production of cellobiose or as a chassis strain for producing cellulase.

The invention provides a high-protein feed raw material and a preparation method thereof, and belongs to the field of protein feeds.The preparation method comprises the steps that animal-derived organs are selected, an animal-derived organ enzyme solution is obtained through a pretreatment means, and natural protein components in the animal-derived organ enzyme solution are reserved to serve as crude protein nutritional ingredients; step 2, adding a substrate and a methyl donor into the animal-derived visceral organ enzyme liquid, and generating guanidinoacetic acid and creatine through an enzymatic synthesis reaction to obtain a mixed liquid; and step 3, carrying out enzyme system inactivation, centrifugal filtration, drying and crushing on the mixed solution to obtain a finished product. According to the invention, guanidinoacetic acid and creatine are synthesized by in-situ catalysis of animal-derived visceral organ natural enzyme systems, and can be naturally fused with protein components, so that the problem of compatibility is avoided, and growth functional factors do not need to be added from an external source; natural enzyme systems of animal livers and kidneys are directly utilized, industrial enzymes do not need to be purchased, and the natural enzyme systems are more adaptive to a raw material system; protein, natural enzyme systems and mineral substances in the raw materials are synchronously utilized, so that one material has multiple purposes.

The invention discloses a method for preparing rare saponin by utilizing panax japonicus endogenous enzyme, and relates to the technical field of extraction, the panax japonicus plant material or crude extract thereof is directly taken as a starting point, the enzyme system is simple and convenient to obtain (fresh medicinal material low-temperature homogenate extraction or direct use of freeze-dried powder), the reaction system is simple, and the operation is easy. According to the preparation process provided by the invention, the catalytic action of endogenous enzyme is fully utilized, so that the introduction of exogenous substances (acid-base, protein and the like) in the extract is completely avoided; the method disclosed by the invention is high in catalytic efficiency, can quickly realize complete conversion of saponin (within 10min) under specific conditions, and has extremely high industrial application.

The present application relates to the technical field of peptidomics research strategy, and in particular to a data analysis ecosystem of single-position peptide clustering based on aa-score method, a construction method thereof and application thereof. The aa-score algorithm provides a new peptidomics research strategy, i.e. single-position peptide clustering, based on the overall change effect of a certain amino acid site of a protein by enzyme system activity, which greatly reduces the redundancy of polypeptide group data, weakens the serious missing value problem of peptidomics, improves the efficiency and accuracy of peptidomics data analysis, and can quickly process large-scale data sets, thereby providing a new research idea for the field of peptidomics research.

The invention provides a yeast rich in polyphosphate and application of the yeast in synthesis of adenosine triphosphate, and the content of polyphosphate is 15-35 wt% based on the dry matter mass of the yeast rich in polyphosphate; according to the method disclosed by the invention, the yeast is subjected to phosphorus starvation culture and then is subjected to phosphorus supplementary culture, so that the yeast is enriched with polyphosphates, and the yeast rich in polyphosphates is obtained; then, the yeast rich in polyphosphate is used as a biocatalyst to synthesize ATP, that is, self-related enzyme systems, mainly including adenosine kinase, endo-polyphosphate kinase and excision-polyphosphate kinase, in the yeast rich in polyphosphate are used for replacing exogenous polyphosphate kinase and adenosine kinase, and in addition, ATP is synthesized by using the yeast rich in polyphosphate as a biocatalyst. According to the method for synthesizing ATP by using the yeast rich in polyphosphate as the biocatalyst, exogenous adenosine kinase and exogenous polyphosphate kinase do not need to be additionally added, the cost is low, the efficiency of converting adenosine into ATP is high, the batch-to-batch stability of the yeast is realized, and the enzyme activity of the self-related enzyme system is high.

The invention relates to the technical field of sugar alcohol preparation, and discloses a preparation method of high-purity crystalline maltitol, which comprises the following steps: firstly, by taking corn starch milk as a raw material, preparing ultrahigh-content maltose syrup with the maltose content of more than or equal to 91%, the wheat triose content of less than or equal to 0.4% and the glucose content of less than or equal to 4% through an enzyme conversion method; carrying out hydrogenation reaction on the syrup by adopting a Raney nickel catalyst, and controlling the hydrogenation side reaction to be 2% or below, so as to obtain hydrogenated liquid with maltitol content of more than or equal to 88%; and then, a pigment separation procedure in a traditional process is omitted, and the hydrogenated liquid is directly subjected to decoloration, ion exchange, evaporation concentration, crystallization, centrifugation and drying treatment to finally prepare crystalline maltitol. According to the method, by screening a specific compound enzyme system and finely regulating the pH value and temperature control environment of a saccharification reaction system, the generation of maltose is effectively promoted, and side reactions such as excessive hydrolysis of glucose and reverse synthesis of trisaccharide are inhibited at the same time, so that the high-purity maltose syrup precursor is obtained.

This invention discloses a method for preparing a carbon source for microbial culture medium based on enzymatic hydrolysis of bamboo powder and its application, belonging to the field of high-value utilization technology of biomass resources. Using bamboo powder as raw material, the invention involves mild alkali pretreatment to disrupt the dense structure of lignocellulose, followed by enzymatic hydrolysis using a specialized enzyme system of endoglucanase Cel7B and xylanase XynA expressed heterologously by *E. coli* in an optimized ratio. After solid-liquid separation, an enzymatic hydrolysate rich in reducing sugars is obtained. The hydrolysate is then nutritionally fortified with trace elements such as sodium thiosulfate and sodium silicate to produce a low-cost, high-efficiency liquid carbon source. This invention achieves high-value utilization of bamboo waste. The entire process is mild, environmentally friendly, and simple, easily scaled up industrially, and solves the industry bottleneck of high carbon source cost in industrial microbial culture, demonstrating good application prospects and market value.

The invention discloses a walnut kernel skin active component enrichment method based on differential pressure blasting puffing and gradient biotransformation, and relates to the technical field of agricultural product deep processing and plant active component extraction, and the method comprises the following steps: S1, carrying out differential pressure blasting puffing treatment on dried walnut kernel skin, maintaining the pressure of the material at 0.8-1.5 MPa for 10-60 seconds, and then instantaneously releasing the pressure to normal pressure within 0.05-0.2 seconds, the porosity is increased, cell walls are broken, and the specific surface area is increased; s2, mixing the puffed walnut kernel skin and water according to a solid-liquid mass ratio of 1: 10-1: 30 to form slurry, adding a compound enzyme system containing cellulase, hemicellulase, pectinase, tannase and / or protease, and performing enzymolysis for 1-4 hours under the conditions that the temperature is 45-55 DEG C and the pH is 4.5-6.0, so that cell wall components are partially hydrolyzed and bound phenols are released; and S3, sequentially carrying out first-stage fermentation and second-stage fermentation on the materials subjected to enzymolysis.

The invention relates to the technical field of plant genetic engineering and agricultural planting, in particular to application and method of an SlGRXC9 gene in improvement of tomato cadmium stress tolerance, the number of the SlGRXC9 gene is Solyc07g053550, the core coding region sequence of the SlGRXC9 gene is SEQ ID NO.1, and the coded protein sequence is SEQ ID NO.2; through construction of SlGRXC9 overexpression transgenic tomatoes, heterologous expression of protein encoded by the gene in plant cells or use of the gene as a molecular breeding target, the system activity of antioxidant enzymes such as SOD, POD, CAT and GR in tomatoes can be enhanced, cadmium stress induced H2O2 and O2-accumulation and membrane lipid peroxidation damage are reduced, and absorption and accumulation of the tomatoes to cadmium ions are not affected; according to the method, the growth ability and physiological stability of the tomatoes under cadmium stress are remarkably improved, the risk of nutrient imbalance is avoided, the hereditary stability is high, and the method can be widely applied to safe production of the tomatoes in cadmium-contaminated soil and creation of stress-tolerant germplasm.

A modified pegRNA Cas9 nickase system that maintains potent on-target editing activity but has reduced off-target editing activity relative to wild-type Cas, and methods of production and use.

This invention provides a multi-component resource utilization process for black soldier fly larvae larvae slurry, relating to the field of insect resource utilization technology. The process sequentially includes raw material pretreatment, compound enzymatic hydrolysis, lactic acid bacteria fermentation, stratified separation, and targeted treatment of each component. The compound enzymatic hydrolysis uses a combined enzyme system of trypsin and papain, and the lactic acid bacteria fermentation uses *Lactobacillus plantarum*. The fermentation parameters are: carbon-to-nitrogen ratio of 20:1, material-to-liquid ratio of 4:1 (w / v), pH of 6.5, fermentation temperature of 37°C, and fermentation time of 24 hours. This process achieves the synergistic recovery and high-value utilization of all components of black soldier fly larvae. A single process can simultaneously produce four types of products: insect oil, water-soluble fertilizer, antimicrobial peptide additives, and antibiotic-free aquatic feed ingredients, significantly improving resource utilization and industrial added value. The entire process involves no organic solvent addition, employs low-temperature enzyme inactivation to reduce energy consumption, is environmentally friendly and energy-saving, and avoids antibiotic resistance issues.

The invention relates to the technical field of agricultural biology, in particular to a method for increasing the germination rate of hemerocallis citrina L. seeds, the method for remarkably increasing the germination rate of the hemerocallis citrina L. seeds through combined treatment of gibberellin GA3 and hydrogen peroxide H2O2 is easy and convenient to operate and low in cost, the physiological dormancy of the seeds is broken through gibberellin, embryo cell division is promoted, and the germination rate of the seeds is increased. Meanwhile, hydrogen peroxide is used for moderately oxidizing seed coats, permeability is improved, active oxygen signal molecules are provided for activating germination related enzyme systems, the hydrogen peroxide and the active oxygen signal molecules generate a synergistic interaction effect through a dormancy breaking-permeation promoting-activation triple mechanism, the germination rate is increased in a breakthrough mode, and the germination period is remarkably shortened.

The invention discloses high-GABA (gamma-aminobutyric acid) active milled rice with embryo and a processing method thereof. Belongs to the technical field of functional cereal food processing. Compared with a conventional enzyme, the novel glutamate decarboxylase GAD-SL19 has excellent temperature and pH tolerance, the stability of a fermentation process is guaranteed, the GABA content of the milled rice with embryo reaches 3.8 g / 100g or above, and the glutamic acid conversion rate is larger than 94%. In addition, by optimizing the process (citric acid short-time soaking, humidity-controlled germination and staged drying), the fissure rate is effectively reduced, and the integrity and cooking taste of rice grains are improved. The germ rice prepared by the method has the characteristics of high GABA activity and low thermosensitive loss, breaks through the limitation of a traditional liquid enzyme system, and provides technical support for high-valued utilization of rice germ resources.

This invention discloses a dual-enzyme system for catalyzing the synthesis of L-cysteine ​​from D-serine and its applications. This dual-enzyme system achieves the asymmetric synthesis of L-cysteine ​​from D-serine with a substrate conversion rate of 99% and a product yield >110%. The catalytic process of this invention is simple, with no byproducts generated and easy subsequent separation. Therefore, it has advantages such as high atom economy, high product optical purity, mild reaction conditions, environmental friendliness, and simple product post-processing. It has great potential for the industrialization of low-cost and controllable multi-enzyme preparation of L-cysteine.

The application discloses a food-like lactobacillus double-plasmid multi-gene editing system based on CRISPR / Cas9 technology and application thereof. The food-like lactobacillus double-plasmid multi-gene editing system comprises (1) a plasmid pCRI01 comprising the following elements: a Cas9 protein coding gene; a temperature-sensitive replicon pSC101; an sgRNA expression module targeting the replicon of the plasmid pCRI02, which is controlled by an inducible promoter Pnis; an ampicillin resistance; and (2) a plasmid pCRI02 comprising the following elements: at least one sgRNA expression module driven by a P23 promoter; a lambda-Red recombinase system comprising Exo, Beta and Gam genes; an erythromycin resistance marker gene; and a kanamycin resistance gene. The multi-gene editing system provided by the application is a small-size pCRI01 and pCRI02 double-plasmid system, and the number of transformed cells after resistance screening is significantly improved; and the gene editing efficiency mediated by the pCRI plasmid is greatly improved with the assistance of the lambda-Red recombinase system.

This invention discloses a neurotrophic brain protein peptide composition and its applications, belonging to the field of food technology. The components of the composition provided by this invention have synergistic effects. On the one hand, they reduce the damage of oxidative stress to the nervous system, improve sleep quality, and create a favorable environment for the repair and regeneration of the nervous system. On the other hand, they provide the raw materials needed for the maintenance, repair, and regeneration of nerve cells, as well as the synthesis of neurotransmitters, ensuring energy supply and the activity of the required enzyme systems, thereby achieving the effect of effectively nourishing / repairing nerves and / or promoting nerve regeneration. The provided composition does not use organic reagents, is environmentally friendly, and has high safety; it has low preparation cost and a good taste.

The present application relates to the field of bioengineering and microbial technology, and relates to a yeast surface display construction method of a double-enzyme system. AGA1 The gene promoter is replaced by a strong constitutive promoter TEF1 and a glucose-responsive promoter HXT7 ; a double-enzyme free expression vector containing a galactose-inducible promoter GAL1 driven detoxification enzyme gene is constructed, and is transformed into a modified chassis cell to obtain a double-enzyme co-display engineering bacterium; the chassis protein AGA1 is expressed in a culture medium, and YtdepA and YtdepB are induced in a galactose-induced culture medium, so that the YtdepA and YtdepB are co-displayed on the yeast surface through the coupling mechanism of AGA1-AGA2. The strong constitutive promoter TEF1 and the glucose-repressed promoter HXT7 are coupled with the galactose-inducible promoter GAL1 respectively, so that the chassis protein and the detoxification enzyme are matched in the expression timing and intensity, and the detoxification enzyme is displayed on the yeast surface.

The application relates to the field of biotechnology, and discloses a method and system for regulating the ratio of amino acids and peptides after soybean proteolysis, which comprises collecting enzyme-coupling parameters in the whole proteolysis process to generate time series data, obtaining a multi-dimensional dynamic covariance matrix based on the data, identifying significant enzyme interaction through the matrix, and generating a list; a multi-level enzyme interaction network model is constructed according to the list, and finally, dynamic and accurate regulation of the ratio of amino acids and peptides is realized based on the model; the application can effectively solve the identification problem of hidden interaction interference in a multi-enzyme system, improve the consistency and predictability of product functions, and reduce the batch-to-batch fluctuation of the content of target functional peptides.

The invention discloses a method for removing and degrading starch by a compound enzyme system in a pulping process of waste box boards, which comprises alpha-amylase, beta-amylase, glucoamylase, pullulanase and pectinase. OCC white water and OCC paper pulp are subjected to enzymolysis through alpha-amylase and another three kinds of compound enzymes (beta-amylase, pullulanase and glucoamylase) which are compounded (the enzyme activity is conventional enzyme activity), and when the dosage of the compound enzymes is 1000 mL * t <-1 >, the enzymolysis rate of the compound enzymes is far superior to that of single alpha-amylase with the dosage of 5000 mL * t <-1 > at the same enzymolysis time; the starch decomposition rate of the waste paper pulp is increased by 3.75 times and reaches 42.8%, and the white water is correspondingly increased by 38% and reaches 42.8%.

Recombinant DNA molecules and constructs are provided that are useful for modulating gene expression in plants. One or more expression cassette(s) of a recombinant DNA molecule or construct may be excised from transgenic plants following transformation by the presence of flanking site-specific recombination sites in the recombinant DNA molecule or construct by expression of a recombinase enzyme encoded by the recombinant DNA molecule or construct. Such a recombinase system may be used to remove such expression cassette(s) from plants transformed with the recombinant DNA construct or vector. The recombinase transgene may be operably linked to a tissue-preferred or tissue-specific promoter for autoexcision in transformed plants without crossing to a different transgenic line expressing the recombinase. Methods for causing autoexcision of one or more expression cassette(s) in a transgenic plant, and plants and cells containing or transformed with a recombinant DNA molecule or construct of the present disclosure, are also provided.

This invention relates to the field of microbial technology, disclosing a *Strombus adhesiveus* strain YF2 that degrades polyethylene mulch film, microbial preparations, methods, and applications, addressing the problems of insufficient efficacy and unclear mechanisms in existing PE-degrading strains. This strain is deposited at the Guangdong Provincial Microbial Culture Collection Center, with accession number GDMCC No:67086, and a deposit date of October 13, 2025. Through systematic parallel comparisons, strain YF2 significantly outperformed the control strain in PE degradation efficiency, biofilm formation ability, and induction of PE polymer oxidation. Genomic analysis showed that YF2 contains a genetic network encoding key degrading enzyme systems such as nitroreductase, cytochrome P450, and alkane monooxygenase, and particularly possesses unique nitrification degradation potential. Microbial preparations prepared based on this strain can be used for the bioremediation of PE mulch film residues in farmland soils, compost, and other environments.

The invention relates to the technical field of fruit and vegetable fermentation, in particular to a brewing method of rose vinegar. The brewing method comprises the following steps: sequentially carrying out hydrolysis, enzymolysis and fermentation treatment on raw materials; the raw materials comprise roses and plant processing residues retaining plant characteristic aroma, and the mass ratio of the roses is not lower than 45%; the enzymolysis comprises the step of carrying out segmented enzymolysis by adopting a compound enzyme; the compound enzyme comprises tannase, beta-glucosidase, pectinase and cellulase, and the enzyme activity ratio of the tannase to the beta-glucosidase to the pectinase to the cellulase is 1: (1.5-2.5): (2.5-3.5): (3.5-4.5). The segmented enzymolysis is performed under the conditions of 38-42 DEG C, 53-57 DEG C, 28-32 DEG C and 23-27 DEG C in sequence. According to the invention, a complex enzyme system is constructed to cooperate with a four-stage temperature-controlled enzymolysis process, so that the rose vinegar which is full in aroma release, stable in flavor, extremely low in astringent component and rich in functional component is provided, and the rose vinegar has important application value.

The invention relates to a thermophilic carbon monoxide streptomyces and a complex microbial inoculant, a preparation method and application thereof, and belongs to the technical field of microorganisms, the classification name of the strain is thermophilic carbon monoxide streptomyces, the name of the strain is G-4-7, and the preservation number of the strain is CGMCC No.36417. The efficient lignin degradation complex microbial inoculant can be prepared from the thermophilic carbon monoxide streptomyces, Brevibacillus agri G-5-2 and Chaetomium thermophilum by using the thermophilic carbon monoxide streptomyces, the name of the strain is G-4-7, and the preservation number of the strain is CGMCC No.36417. The complex microbial inoculant can efficiently degrade bagasse lignin in a high-temperature environment, enzyme systems of all strains are complementary, the degradation efficiency is synergistically improved, resource utilization of bagasse rich in lignin can be promoted, and a technical basis is provided for a green treatment mode of agricultural waste.

The present application relates to the technical field of microorganism or culture thereof processing, and particularly relates to a method for breeding pleurotus ostreatus mycelium by using highland barley as a substrate. The method is as follows: highland barley is crushed and sieved, then water is added to prepare highland barley slurry, and a complex enzyme system composed of amylase and cellulase is added to perform liquid enzymolysis to obtain an enzymolysis substrate; lactic acid bacteria strains are inoculated into the enzymolysis substrate to perform solid-state fermentation to obtain a fermentation substrate; the fermentation substrate is subjected to moist heat sterilization treatment to obtain a sterilized substrate; the sterilized substrate is inoculated with pleurotus ostreatus original seeds, and is placed in a culture environment for culture to obtain pleurotus ostreatus mycelium. The scheme breaks the physical barrier of the fiber network of the highland barley epidermis and the starch crystal structure of the endosperm by using the complex enzyme system and lactic acid bacteria fermentation, and the substrate is pre-degraded before the mycelium is inoculated, so that the mycelium can directly absorb free carbon sources, the germination time is shortened, the daily growth rate is improved, the residual sugar rate of the substrate is reduced, and the dry weight biomass of the mycelium is increased.