427 results about "Enzyme system" patented technology

Glucose and ketones may be dysregulated singularly or concurrently in certain physiological conditions and may be advantageously assayed together using an analyte sensor capable of detecting both analytes. Certain analyte sensors capable of dual detection may comprise a first working electrode and a second working electrode, a ketones-responsive active area disposed upon a surface of the first working electrode, a glucose-responsive active area comprising a glucose-responsive enzyme disposed upon a surface of the second working electrode, a membrane having a first portion overcoating the ketones-responsive active area and a second portion overcoating the glucose-responsive active area, in which the first portion and the second portion have different compositions. The ketones-responsive active area comprises an enzyme system comprising at least two enzymes that are capable of acting in concert to facilitate detection of ketones.

The invention discloses a kit for detecting tomato brown crinkled fruit virus based on enzyme-mediated dual-amplification nucleic acid amplification and a detection method. The detection system comprises a primer pair DNA (deoxyribonucleic acid) upstream primer (F5) and a DNA downstream primer (R6) for amplifying the tomato brown crinkled fruit virus movement protein gene, a probe (RNA primer (RNA1)) for detecting the MP gene, a nucleic acid amplification group enzyme system (recombinase, single-chain binding protein, polymerase and ATP (adenosine triphosphate) energy regeneration enzyme), a signal amplification group enzyme system (positioning enzyme and cutting enzyme), activating enzyme NTP, a buffer solution and RNase-free Water. The system composed of the freeze-drying protective agent is prepared into the pre-subpackaged freeze-dried balls. The kit disclosed by the invention can realize DNA detection of aM concentration, and besides rapid qualitative detection of the tomato brown crinkled fruit virus, early screening of the tomato brown crinkled fruit virus can also be realized due to high detection sensitivity.

The invention discloses a method for detecting antioxidants and polyphenols in food by constructing a colorimetric sensing array based on monatomic iron nano-enzyme, and belongs to the field of nanotechnology and colorimetric sensing. The traditional monatomic nano enzyme shows limited enzyme-like activity and substrate specificity, and is difficult to meet the comprehensive requirements of detecting various antioxidants and polyphenols in food. In the research, a novel nano-enzyme system (sdsFeN (at) G) of monatomic iron nano-enzyme is developed. The SdsFeN (at) G shows excellent enzymatic activity, and a three-channel colorimetric sensing array is constructed by utilizing inhibition of different antioxidants and polyphenols on oxidation of three chromogenic substrates, so that simple, convenient and reliable identification and detection of five substances (tert-butylhydroquinone, butylated hydroxyanisole, propyl gallate, catechol and hydroquinone) in food are realized.

The invention relates to the technical field of plant polysaccharide extraction, in particular to a process for synergistically extracting rape pollen polysaccharide by a compound enzyme, which comprises the following steps: crushing rape pollen to 200-300 meshes by pulse airflow, performing microwave-ultrasonic degreasing by using a 85% ethanol solution, mixing with an acetic acid-sodium acetate buffer solution with the pH value of 4.8, and performing ultrasonic extraction to obtain a crude extract; the method comprises the following steps: adding a compound enzyme system which is immobilized by Fe3O4 (at) SiO2 nanoparticles by cellulase, pectinase, protease and beta-glucosidase according to a mass ratio of 2: 1: 1: 0.5, carrying out enzymolysis for 4 hours in a 50 DEG C constant-temperature water bath and a 15V / cm pulsed electric field, carrying out enzyme deactivation on enzymatic hydrolysate, carrying out gradient ethanol precipitation, carrying out ultrafiltration deproteinization, and carrying out vacuum pulsation freeze drying to obtain the polysaccharide. According to the method disclosed by the invention, the cell wall breakage rate and the enzymolysis efficiency are improved through pulsed airflow crushing, magnetic nano immobilized enzyme and pulsed electric field assistance, the polysaccharide yield reaches 7.32%, the purity is 91%, the beta-glucosidic bond proportion and the antioxidant activity are remarkably improved, the enzyme can be recycled and reused, the drying time is shortened, and efficient and low-consumption extraction is realized.

The invention discloses a PET hydrolase mutant as well as a preparation method and application thereof, and belongs to the field of enzyme engineering. On the basis of FAST-PETase, in combination with deep learning prediction and enzyme engineering optimization strategies, a plurality of mutants with specific performance advantages are systematically constructed and screened, the method is suitable for application scenes with different temperatures, substrate loads and degradation efficiency requirements, and experimental basis and technical support are provided for constructing a diversified and combinable PET degrading enzyme system. The mutants show diversified advantages in different performance dimensions, for example, the enzyme activity of the K233R mutant is 1.62 times that of FAST-PETase, and the K233R mutant still keeps good thermal stability (1.03 times) after being subjected to heat treatment for 2 hours at the temperature of 50 DEG C; q224K is more remarkably improved in the aspect of thermal stability, and the residual activity after heat treatment is 1.64 times that of FAST-PETase.

The invention provides a composition, a kit and a detection method for detecting staphylococcus aureus or a drug-resistant gene thereof based on RPA-CRISPR / Cas12a and application of the composition, the kit and the detection method, and belongs to the technical field of gene detection. A CRISPR / Cas12a system is combined with recombinase polymerase amplification (RPA), an RPA-CRISPR / Cas12a detection method for rapidly detecting the staphylococcus aureus in the wastewater and the drug-resistant gene of the staphylococcus aureus is established, a specific primer and crRNA are designed, the high-specificity nucleic acid cutting capability of a gene editing enzyme CRISPR / CAS system and the isothermal rapid amplification advantage of a nucleic acid isothermal amplification technology are fully fused, and the detection method has the advantages of high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity The fluorescent probe has high-specificity sequence recognition capability, realizes efficient detection of target nucleic acid, is strong in detection specificity, high in sensitivity and simple and convenient to operate, does not need complex equipment and professionals, and is suitable for rapid detection in an on-site environment with limited resources.

The invention belongs to the technical field of green processing of biomass materials, and particularly relates to a green preparation process of high-deacetylation-degree chitin. The preparation method aims at the problem of high energy consumption of existing high-deacetylation-degree chitin preparation. According to the method, raw materials are pretreated by utilizing a compound enzyme system under a low-temperature condition, chitin crystal lattices are preliminarily dissociated by combining a deep eutectic solvent at a medium temperature, high deacetylation conversion is realized through short-time dissolution of an ionic liquid, and meanwhile, a membrane separation-electrodialysis-gradient freezing crystallization three-stage recycling technology is matched, so that recycling of the solvent is realized; the method greatly reduces the reaction temperature and energy consumption, avoids the use of corrosive reagents, and has the advantages of environmental protection, low energy consumption, high deacetylation degree of the product, excellent quality and the like.

The invention provides a cobalt monatomic nano-enzyme system modified by phospholipid polyethylene glycol carboxyl. The cobalt monatomic nano-enzyme system comprises a cobalt monatomic nano-enzyme composite core and phospholipid polyethylene glycol carboxyl compounded on the surface of cobalt monatomic nano-enzyme, the cobalt monatomic nano-enzyme composite core comprises a cobalt monatomic nano-enzyme and ginsenoside loaded on the cobalt monatomic nano-enzyme. The invention also provides a bifidobacterium synergetic cobalt monatomic nano-enzyme system which is formed by combining cobalt monatomic nano-enzyme with ginsenoside Rb1 and further delivering the cobalt monatomic nano-enzyme and ginsenoside Rb1 through bifidobacterium bifidum for targeted therapy of enteritis. The combination of Co SA and Rb1 can enhance the anti-inflammatory efficacy by adjusting a key immune signal pathway, and bifidobacterium bifidum can ensure targeted delivery of microbiota and promote intestinal barrier repair. The synergistic three-component strategy integrating monatomic nano-enzyme antioxidation, natural drug immunoregulation and probiotic targeting is provided, and a promising treatment platform is provided for accurate intervention of IBD.

The invention discloses a kit for simultaneously detecting listeria monocytogenes and a drug-resistant gene fosX, and belongs to the technical field of bacterial molecular diagnosis. In order to solve the technical problems that in the prior art, a traditional method for detecting the listeria monocytogenes and the drug-resistant genes fosX of the listeria monocytogenes is low in efficiency and high in equipment dependence degree, the invention provides a kit for simultaneously detecting the listeria monocytogenes and the drug-resistant genes fosX of the listeria monocytogenes, and the kit comprises an EmDEA specific primer pair and an RNA probe, and a nucleic acid amplification group enzyme system and a signal amplification group enzyme system. The kit for simultaneously detecting the listeria monocytogenes and the drug-resistant gene fosX of the listeria monocytogenes, provided by the invention, has relatively strong specificity, repeatability, stability and reliability, and the sensitivity can be as low as 500 copies / mu L. The kit provided by the invention is simple in operation process, can be carried to rural sites, remote areas or any other scenes needing instant detection for detection, and has a wide application prospect.

The invention provides a method for preparing rebaudioside M by constructing a UGT91C1-UGT76G1-SuSy fusion enzyme on the basis of an artificially constructed substrate microchannel principle and using relatively cheap raw materials to efficiently catalyze a cascade reaction. EAAAK 3 and (GGGGS) 4 are used as connecting peptides, UGT76G1 is fused at the C end of UGT91C1, SuSy is fused at the C end of UGT76G1, mass transfer of an intermediate product is enhanced, and the intermediate product is prevented from being diffused to a reaction main body. The invention also discloses a construction method of the ugt91C1-C1-ugt76G1-suusy fusion gene, a construction method of a recombinant plasmid, a construction method of a recombinant strain, a protein expression and purification method and a method for catalytically preparing a product. The ugt91C1-C1-ugt76G1-suusy fusion gene has a very good application prospect in biological industrial catalytic production.

The invention relates to the technical field of food processing, and particularly discloses soybean flavor peptide and a preparation method and application thereof. Aspergillus oryzae is selected as a leavening agent, a microbial endogenous enzyme generated by full fermentation of the aspergillus oryzae is utilized to cooperate with a compound enzyme preparation to perform combined action, abundant enzyme systems effectively perform enzymolysis on macromolecules such as protein, and related taste substances are provided for subsequent enzymolysis products; in the soybean flavor peptide prepared by enzyme hydrolysis and Maillard reaction, the contents of target peptide and target amino acid (umami peptide, umami amino acid and the like) are high; when the soybean flavor peptide is applied to the compound seasoning with the main function of increasing freshness, the freshness score can be increased by 0.5-2 times. In addition, on the basis of improving freshness, the taste richness of the food or the compound seasoning can be enhanced, and the aftertaste time is prolonged.

The invention discloses a modular fluorescent RNA aptamer biosensor system, and relates to the field of medicine. Comprising a probe module, an enzyme system, a dye and a reaction buffer solution, the probe module comprises a promoter probe P and a reporter probe R, the enzyme system comprises SplintR ligase and T7RNA polymerase, and the reaction buffer solution comprises a transcription buffer solution and a SplintR ligase buffer solution. The promoter probe P comprises a T7 promoter sequence and a 5 '-phosphorylated upstream recognition region, and the length of the promoter probe P is 10nt. According to the present invention, the ligase-assisted probe assembly and the T7RNA polymerase-mediated fluorescent RNA aptamer transcription are integrated, the simultaneous detection of the multiple circRNA can be achieved without the complex probe labeling, the femtomole-level sensitivity and the excellent single base mutation distinguishing ability in the complex sample are provided, and the real-time fluorescence instrument and the portable device are adapted.

The invention discloses a fresh plant directed biotransformation method based on endogenous enzyme activation and a product and application thereof. The core of the preparation method is as follows: fresh plants (tuberous roots, stems or fruits) which are harvested in a ripe state and have metabolism in a dormant state (the respiration rate is lower than 5 mg CO / kg.h) are taken as raw materials, the raw materials are treated in an environment with the specific pressure being 0.02-0.20 MPa, the temperature being 70-121 DEG C and the humidity being 50-95%, a specific impregnating compound can be selectively combined, and the raw materials can be prepared into the plant culture medium. Under the conditions that exogenous microorganisms are not introduced and plant tissue structures are not damaged, the catalytic function of an endogenous metabolic enzyme system is safely and efficiently restarted and maximally promoted, and components of a plant body are driven to realize directional biotransformation. According to the process, conversion of primary metabolites and / or secondary metabolites is remarkably promoted, the content conversion of one or more beneficial components in the primary metabolites and / or the secondary metabolites is improved, and meanwhile, the biological and pharmaceutical structures and inherent metabonomics characteristics of the plants are completely reserved. The biotransformation product can be used as a high-quality raw material to be applied to medicines, common foods, health foods, special diet foods, daily chemical products and disinfection products, and can be further prepared into traditional Chinese medicine decoction pieces, solid preparations, semi-solid preparations and liquid preparations by adding or not adding pharmaceutically acceptable auxiliary materials.

The invention discloses nano-enzyme coating recombinant probiotics as well as a preparation method and application thereof, and belongs to the technical field of biotechnology and nano medicine. According to the invention, engineered Escherichia coli Nissle 1917 (ECN (DE3)) is taken as a host, and glutathione peroxidase (GPX) and glutathione reductase (GR) genes are over-expressed through a genetic engineering means, so that an efficient antioxidant enzyme system is constructed. And sequentially modifying chitosan and cerium dioxide nano-enzyme (CNP) on the surfaces of the thalli by using an electrostatic layer-by-layer self-assembly technology to form a protective coating. The nano-enzyme coating significantly improves the survival rate of the recombinant probiotics in severe environments such as simulated gastric acid (pH 2.5) and bile salt (0.3%), and enhances the ability of the recombinant probiotics to remove reactive oxygen species (ROS), including hydrogen peroxide, superoxide anions, hydroxyl free radicals and DPPH free radicals.

The invention discloses a co-fermentation method based on sphingomonas and a rhodiola rosea extracting solution and application of a product, and belongs to the technical field of microorganisms. By utilizing a special rich enzyme system of sphingomonas and combining with an early-stage composite enzymolysis process and synergistic effect, the wall breaking efficiency of rhodiola rosea cell walls and the conversion degree of active components of rhodiola rosea are greatly improved, the bottlenecks of low efficiency and low bioavailability of a traditional rhodiola rosea extraction method are successfully overcome, and the rhodiola rosea extraction method is suitable for industrial production. The functional components such as salidroside and flavone can be fully released and converted. Secondly, different from non-symbiotic bacteria (such as lactic acid bacteria and saccharomycetes) which are commonly used for fermentation in the prior art, a potential symbiotic bacterium of sphingomonas XJL-TP07 from a special environment is innovatively selected and has better metabolic suitability with the rhodiola rosea component, the fermentation process is milder and safer (no irritant is produced), and the rhodiola rosea fermentation method is suitable for large-scale production. And a synergistic interaction effect can be generated through a unique biotransformation path.

The invention relates to the technical field of oral medicine, in particular to a method for dynamically monitoring the enamel demineralization risk of an orthodontic patient based on a salivary enzyme activity spectrum, and the method comprises the following steps: S1, obtaining a saliva sample from the orthodontic patient at least twice in an orthodontic treatment process; s2, treating the saliva sample to obtain a standardized saliva sample; s3, analyzing the standardized saliva sample to obtain at least two different enzymatic characteristic indexes of at least one enzyme or enzyme system in the saliva, and reflecting multiple molecular characteristics of the enzyme or enzyme system or response of the enzyme or enzyme system to environmental change through the enzymatic characteristic indexes; and S4, dynamically evaluating the enamel demineralization risk of the orthodontic patient based on the change of the enzymological characteristic indexes obtained at different time points. By analyzing multi-dimensional molecular behaviors and dynamic changes of enzymes or enzyme systems in saliva, the method aims at overcoming the defects of the prior art in the aspects of early warning ability, dynamic nature and individualized evaluation, and early, dynamic and individualized monitoring of the enamel demineralization risk of an orthodontic patient is achieved.

The invention discloses a siraitia grosvenorii pomace extract as well as an extraction method and application thereof. Relates to the technical field of biological fermentation and natural product extraction. Comprising the following steps: strain activation and mixed culture; biological fermentation pretreatment; decoloring and eluting; and enriching and drying. Through enzyme system complementation of compound microorganisms, cell wall structures of the siraitia grosvenorii residues are degraded in stages, saponin, polyphenol, flavone and polysaccharide are synchronously released, and the biological activity is remarkably improved. The layer-by-layer decomposition of the siraitia grosvenorii residue fiber structure is realized by adopting sectional fermentation, and the dissolution of functional components is promoted; ultrasonic synergistic auxiliary extraction is combined, so that efficient wall breaking and component dissolution are realized; the steps of ultrasonic extraction, macroporous resin decoloration and the like are designed in series, so that the solvent dosage is reduced by 50%, and the energy consumption is reduced by 35%; the gradient elution realizes simultaneous collection of functional components such as saponin, flavone, polyphenol and the like. The extract can be directly used for functional food, medicines or cosmetics, and the fermentation liquor can be processed into the momordica grosvenori flavored lactic acid bacteria drink.

The invention relates to the technical field of nano-materials, in particular to a monatomic nano-enzyme drug delivery system for IBD treatment and a preparation method and application of the monatomic nano-enzyme drug delivery system. The monatomic nano enzyme delivery system provided by the invention comprises an iron-doped monatomic nano enzyme Fe-SA, curcumin Cur and hyaluronic acid grafted dopamine HAD, the Fe-SA and the Cur form a Fe-SA / Cur compound through a coordination effect, and the surface of the Fe-SA / Cur compound is coated with the HAD. According to the iron-doped monatomic nano-enzyme Fe-SA, Fe atoms in the iron-doped monatomic nano-enzyme Fe-SA are distributed in a graded carbon-based matrix of the Fe-SA in a Fe-N4 co-doping form, and the iron-doped monatomic nano-enzyme Fe-SA has efficient SOD and CAT nano-enzyme activity, and can realize antioxidant self-cascade nano-enzyme reaction, effectively remove active oxygen and improve the pathological environment. According to the preparation method disclosed by the invention, a coordination compound Fe-SA / Cur is constructed by utilizing the iron-doped monatomic nano-enzyme and the curcumin, so that the anti-inflammatory effect is improved, and the characteristic of poor water solubility of the curcumin is effectively improved. According to the invention, HAD is loaded on the outer layer of a coordination compound Fe-SA / Cur to construct an oral monatomic antioxidant cascade nano-enzyme system, high acid resistance and targeted delivery capability are realized by using the remarkable stability and negative charges of HAD, and oral administration is effectively realized. In-vivo and in-vitro experiments show that the monatomic nano-enzyme delivery system provided by the invention can effectively remove intestinal free radicals, improve the intestinal environment, reduce the expression of inflammatory factors, promote the recovery of damaged tissues and effectively treat colitis. The invention provides a new thought and method for biomedical research and drug development of inflammatory bowel diseases.

The invention belongs to the technical field of viral nucleic acid molecular diagnosis, and particularly relates to a kit for detecting cucumber green mottle mosaic virus based on enzyme-mediated dual-amplification nucleic acid amplification, a detection method and application. The detection system comprises a primer pair DNA upstream primer (SEQ ID NO.2) and a DNA downstream primer (SEQ ID NO.3) for amplifying a coat protein gene of the cucumber green mottle mosaic virus, and a probe (RNA primer (SEQ ID NO.1)) for detecting a CP gene, a nucleic acid amplification group enzyme system, a signal amplification group enzyme system, a nucleic acid scavenging enzyme, an activating enzyme NTP, a buffer solution and a freeze-drying protective agent, wherein the system is prepared into pre-subpackaged freeze-dried balls. The kit provided by the invention can realize RNA detection of aM concentration, and can realize early screening of cucumber green mottle mosaic virus due to high detection sensitivity.

The present disclosure relates to compositions and methods for modifying target nucleic acids, and methods for detecting nucleic acids. Various compositions are described herein, including compositions comprising an endonuclease, an endonuclease system, and a chimeric protein having the endonuclease and a nucleic acid modulating domain or a nucleic acid modifying domain.

The invention belongs to the technical field of DNA amplification, and relates to a mixed enzyme system capable of amplifying circular DNA as well as application and a product of the mixed enzyme system. The invention provides a mixed enzyme system capable of amplifying circular DNA (deoxyribonucleic acid). The mixed enzyme system comprises a polymerase III tau clip loader and a catalytic core compound. The mixed enzyme system provided by the invention is remarkably superior to a traditional method in the aspects of efficiency, cost, safety, applicability, sensitivity and the like, provides a brand new solution for efficient preparation of the circular DNA, and has a wide application prospect in the field of biotechnology.

The disclosure relates to compositions and methods that modify target nucleic acids, as well as methods of detecting nucleic acids. Various compositions are described herein, including compositions comprising endonucleases, endonuclease systems, and chimeric proteins having the endonuclease and a nucleic-acid modulating domain or a nucleic acid modifying domain.

The invention relates to a preparation method of dissolving pulp with uniform cellulose polymerization degree distribution, and belongs to the technical field of dissolving pulp. The preparation method of the dissolving pulp comprises the following steps: (1) raw material pulp pretreatment and grading screening; (2) cold alkali swelling and pulsed electric field cooperative treatment; (3) selective enzymolysis treatment of a compound enzyme system of cleavage enzyme and excision enzyme; and (4) neutralizing and washing the slurry. Compared with the prior art, the preparation method of the dissolving pulp provided by the invention has the advantages that the centralization of a polymerization degree main distribution interval (such as 800-1000) is realized, the standard deviation is obviously reduced, and the cellulose polymerization degree distribution is narrower and more controllable. No organic solvent or strong oxidant is needed in the whole preparation process of the dissolving pulp, the alkali liquor can be recycled, the enzyme preparation can be repeatedly used, and good environment friendliness is achieved.

The invention discloses a construction method of an escherichia coli mutant for producing succinic acid by fermentation of a synthetic medium. The method comprises the following steps: firstly, knocking out a lactic dehydrogenase gene ldhA, a pyruvate formate lyase gene pflB, a ptsG gene responsible for encoding a phosphotransferase system EIIBC protein, an ethanol dehydrogenase gene adhE, an acetokinase-phosphate transacetylase gene ackA-pta, and a ptsG gene responsible for encoding a phosphotransferase system EIIBC protein in escherichia coli; a phosphoenolpyruvate carboxykinase gene pck from bacillus subtilis is integrated at an SS9 safety site of a strain to obtain escherichia coli ESC6 with high succinic acid yield; and mutating one or more loci in one or more genes of a glucose-transcriptional inhibition factor gene mlc, a nitrate response regulatory factor gene narL and a cyclic adenylate receptor protein gene crp to obtain the escherichia coli mutant capable of producing succinic acid by fermentation of a synthetic culture medium, wherein the one or more loci in one or more genes of the glucose-transcriptional inhibition factor gene mlc, the nitrate response regulatory factor gene narL and the cyclic adenylate receptor protein gene crp are mutated. The strain can be fermented in a synthetic medium to produce succinic acid, so that the fermentation cost is greatly reduced, and the strain has a very wide application prospect.

The application belongs to the technical field of polymer material recycling, and particularly relates to a high-efficiency enzymatic hydrolysis process of polycaprolactam. The high-efficiency enzymatic hydrolysis process of polycaprolactam provided by the application comprises the steps of acid pretreatment, enzymatic catalytic depolymerization and product post-treatment. The process combines sulfuric acid and a multi-enzyme system to treat PA6 with high substrate concentration. The physical form and chemical accessibility of PA6 are significantly changed through sulfuric acid pretreatment, which creates conditions for subsequent enzymatic hydrolysis reaction, and realizes efficient, deep and green degradation of PA6.

The present invention belongs to the field of genetic engineering and specifically provides a protein PeuENH1 for improving plant salt tolerance. The amino acid sequence of the protein PeuENH1 is shown in SEQ ID NO.2. The protein PeuENH1 can improve plant tolerance to salt stress, reduce plant sensitivity to salt stress, participate in plant response to salt stress, reduce reactive oxygen content, H2O2 content and malondialdehyde content in transgenic plants under salt stress, increase proline content in transgenic plants, enhance the antioxidant enzyme system, reduce oxidative damage to the membrane system by reactive oxygen, thereby improving plant tolerance to salt stress. The protein has important theoretical and practical significance for improving saline-alkali land environment, increasing saline-alkali land utilization efficiency and expanding saline-alkali land vegetation coverage area.

In the present application, by knocking out the BG gene in Trichoderma reesei PX3 (a strain of cellulase transcription activator that can be activated continuously), the BG activity can be reduced under the condition of not reducing or even increasing the filter paper enzyme activity of the enzyme system, and a low BG enzyme activity and high filter paper enzyme activity strain is constructed. This strategy can avoid the negative impact on cellulase production after BG knockout, and the obtained strain can be used for efficient production of cellobiose or as a chassis strain for producing cellulase.

The invention discloses a method for producing soy sauce by using termitomyces albuminosus, and belongs to the technical field of soy sauce production. Aspergillus oryzae and aspergillus niger are subjected to multi-generation domestication screening through a termitomyces albuminosus powder culture medium, the adaptability of the strain to a termitomyces albuminosus substrate is remarkably improved, termitomyces albuminosus has antioxidant activity, the domesticated strain can fully utilize nutritional ingredients in the termitomyces albuminosus in the fermentation process, generation of termitomyces albuminosus flavor substances is promoted, and the yield of the termitomyces albuminosus flavor substances is increased. A compound enzyme system of neutral protease, cellulase, flavourzyme and laccase is adopted, gradient enzyme deactivation and ultrasonic-assisted treatment are combined, cell walls and protein of termitomyces albuminosus are fully decomposed, free amino acid, polysaccharide and flavoring substances are released, the enzymolysis efficiency is improved, and the flavor of the termitomyces albuminosus is improved; through secondary saccharification of the red date polysaccharide extract and beta-glucanase, the content of functional polysaccharide and soluble sugar in the soy sauce is increased, the sour taste of the soy sauce is balanced, and the taste roundness is improved.

Compositions and methods are provided for variant Cas systems and elements comprising such systems, including, but not limiting to, Cas endonuclease variants, guide polynucleotide / Cas endonuclease complexes comprising Cas endonuclease variants, as well as guide polynucleotides and guide RNA elements that can interact with Cas endonuclease variants. Compositions and methods are provided for genome modification of a target sequence in the genome of a cell. The methods and compositions employ a guide polynucleotide / Cas endonuclease system comprising a Cas9 endonuclease variant to provide an effective system for modifying or altering target sequences within the genome of a cell or organism.