88 results about "Ethanol dehydrogenase" patented technology

The invention provides application of acidophilous glycosyltransferase in salidroside production, and belongs to the technical field of biological engineering. The problem of producing salidroside under the acidic condition is solved. Comprising an application of acidophilous glycosyl transferase with an amino acid sequence as shown in SEQ ID NO.1 in salidroside production under an acidic condition and an acidophilous escherichia coli engineering strain for producing salidroside. The escherichia coli engineering strain overexpresses a mutant 3-deoxy-D-arabinoheptulose-7-phosphate (DAHP) synthetase gene aroGfbr, a cyclohexadiene dehydrogenase gene tyrC, a glucose phosphate mutant enzyme gene pgm and a UDP-glucose pyrophosphorylase galU, overexpresses a phenylpyruvate decarboxylase gene ARO10 derived from saccharomyces cerevisiae, and can be used for producing a mutant 3-deoxy-D-arabinoheptulose-7-phosphate mutant enzyme. The kit comprises an ethanol dehydrogenase gene ADH6 and a glycosyl transferase gene LrUGT85AF8. The method is mainly used for producing salidroside under an acidic condition.

The invention discloses a genetically engineered probiotic composition for expressing ADH (adenosine dihydrogenase) and ALDH (aldehyde dehydrogenase) and application of the genetically engineered probiotic composition in hangover alleviating and liver protection. The genetically engineered probiotic composition (such as EcN.1917-ADH and EcN.1917-ALDH) is constructed by introducing genes of alcohol dehydrogenase (ADH, NCBI (National Center for Biotechnology Information) Gene ID: 855349) and acetaldehyde dehydrogenase (ALDH, NCBI Gene ID: 855206) of saccharomyces cerevisiae into probiotics (such as escherichia coli). The engineering bacterium shows remarkable ethanol, acetaldehyde and endurance capacity in an in-vitro experiment, and the ethanol degradation rate is remarkably higher than that of a wild type strain; a mouse drunkenness model verifies that drunkenness and death caused by drinking can be reduced through pretreatment, drunkenness tolerance time is remarkably prolonged, and movement coordination disorder caused by alcohol can be improved. The strain can effectively promote alcohol metabolism and relieve alcoholic liver injury, and a new candidate strain is provided for development of a hangover-alleviating and liver-protecting viable bacterial preparation.

The invention discloses a construction method of an escherichia coli mutant for producing succinic acid by fermentation of a synthetic medium. The method comprises the following steps: firstly, knocking out a lactic dehydrogenase gene ldhA, a pyruvate formate lyase gene pflB, a ptsG gene responsible for encoding a phosphotransferase system EIIBC protein, an ethanol dehydrogenase gene adhE, an acetokinase-phosphate transacetylase gene ackA-pta, and a ptsG gene responsible for encoding a phosphotransferase system EIIBC protein in escherichia coli; a phosphoenolpyruvate carboxykinase gene pck from bacillus subtilis is integrated at an SS9 safety site of a strain to obtain escherichia coli ESC6 with high succinic acid yield; and mutating one or more loci in one or more genes of a glucose-transcriptional inhibition factor gene mlc, a nitrate response regulatory factor gene narL and a cyclic adenylate receptor protein gene crp to obtain the escherichia coli mutant capable of producing succinic acid by fermentation of a synthetic culture medium, wherein the one or more loci in one or more genes of the glucose-transcriptional inhibition factor gene mlc, the nitrate response regulatory factor gene narL and the cyclic adenylate receptor protein gene crp are mutated. The strain can be fermented in a synthetic medium to produce succinic acid, so that the fermentation cost is greatly reduced, and the strain has a very wide application prospect.

The invention relates to the technical field of genetic engineering, in particular to a recombinant microorganism and application thereof in preparation of glycine. The application comprises the following steps: producing glycine by adopting the recombinant microorganism; the recombinant microorganism comprises an enzyme combination and / or a nucleic acid molecule combination. The enzyme combination comprises threonine aldolase, ethanol dehydrogenase and isopropanol dehydrogenase; the threonine aldolase comprises an amino acid sequence as shown in SEQ ID NO. 1. The nucleic acid molecule combination is used for encoding the enzyme combination. According to the invention, threonine aldolase, ethanol dehydrogenase and isopropanol dehydrogenase are converted into microorganisms, and the constructed recombinant microorganisms can be used for decomposing threonine to produce glycine; and a specific system formed by specific isopropanol dehydrogenase, ethanol dehydrogenase and isopropanol dehydrogenase can also have a relatively high conversion rate under the condition of no addition of pyridoxal phosphate (PLP), and has important significance and economic value.

The invention belongs to the technical field of microorganisms, and particularly relates to lactobacillus paracasei BL19 and application thereof to preparation of products with functions of dispelling effects of alcohol and protecting liver. The lactobacillus paracasei BL19 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the lactobacillus paracasei BL19 is CGMCC No.34368. The lactobacillus paracasei BL19 is named as Lactobacillus paracasei BL19. The lactobacillus paracasei BL19 provided by the invention has relatively strong alcohol tolerance, alcohol degradation capability and relatively high activity of promoting alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH); the lactobacillus paracasei BL19 has the advantages that the lactobacillus paracasei BL19 has good anti-alcohol and anti-liver effects and strong capacity of protecting alcoholic injury liver cells, so that the anti-alcohol and anti-liver effects can be comprehensively achieved from multiple dimensions, and in addition, the lactobacillus paracasei BL19 has strong acid resistance and bile salt resistance, also has strong tolerance to simulated gastrointestinal fluid and has important application value in the fields of anti-alcohol and anti-liver products and the like.

The present invention discloses a method for synthesizing hydroxytyrosol by multi-enzyme cascade, comprising: 1) preparing a hydroxytyrosol product by using 3,4-dihydroxybenzaldehyde, L-threonine, sodium formate, NAD + As raw material, L-threonine transaldolase, alcohol dehydrogenase, and formate dehydrogenase are used to catalyze the synthesis of L-threo-3-(3,4-dihydroxyphenyl)serine; 2) L-threo-3-(3,4-dihydroxyphenyl)serine is used as substrate, glucose, NAD + Hydroxytyrosol is synthesized through a cascade catalysis of phenylserine dehydratase, α-ketoacid decarboxylase, aldehyde reductase, and glucose dehydrogenase. The synthesis method provided by the present invention achieves a hydroxytyrosol yield of >99% and a space-time yield of 0.88 g / L / h, currently the highest, showing great potential for industrial application and suitable for further promotion and application.

The invention relates to the technical field of gene engineering, in particular to an ethanol response type gene engineering alcohol-dissolving probiotic as well as a construction method and application thereof. According to the probiotics provided by the invention, an ethanol-responsive regulation mechanism is innovatively introduced, so that the strain can specifically induce high-efficiency expression of human ethanol dehydrogenase 1B (hADH1B) and human acetaldehyde dehydrogenase 2 (hALDH2) when sensing the existence of ethanol in vivo, thereby realizing on-demand and high-efficiency decomposition of ethanol and toxic metabolite acetaldehyde thereof. Compared with continuous expression of traditional engineering bacteria, the intelligent regulation and control mode can better simulate the natural metabolic rhythm of a human body, solves the problem of bacterial metabolism, reduces the metabolic pressure of thalli while improving the hangover alleviating efficiency, and is expected to achieve a better hangover alleviating effect and higher biological safety.

The invention relates to a new application of early-stage chick embryo amniotic fluid, which is characterized in that the early-stage chick embryo amniotic fluid is found to be capable of effectively dispelling the effects of alcohol, removing the content of ethanol in serum and increasing the levels of liver acetaldehyde dehydrogenase and ethanol dehydrogenase by evaluating the effect of dispelling the effects of alcohol of the early-stage chick embryo amniotic fluid, and also has the effects of protecting the gastric mucosa function and the like.

This invention relates to the field of functional food technology, and more particularly to the application of safflower honey, or safflower honey extract, in enhancing the activity of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH). The invention has found that safflower honey, or safflower honey extract, can effectively enhance ADH and ALDH activity, thereby promoting alcohol metabolism and achieving a hangover-relieving effect. Further research on the extract revealed that a mixture of syringaldehyde, riboflavin, lutein, luciferin, and three p-coumaroyl spermidine plays a key role in enhancing ADH and ALDH activity, thus providing an application of this mixture in hangover relief. The application of safflower honey and its extract provided by this invention has high application value for developing foods and health products with hangover-relieving effects.

The present application provides a kind of kudzu vine cranberry composition for protecting liver from alcohol, which contains the following ingredients by weight: 30-50 parts of extract of Hovenia dulcis Thunb, 30-50 parts of mixed extract, 20-40 parts of extract of Pueraria lobata, 10-20 parts of extract of Poria cocos, 10-20 parts of extract of Glycyrrhiza uralensis, 0.05-0.1 parts of vitamin C; the raw materials of the mixed extract are mixed by cranberry and Fructus Lycii in a mass ratio of (1-2):(1-2). The present application further provides formulations and food containing the composition. The present application further protects the use of the composition or formulation in preparing health food or medicine with auxiliary protective effect on chemical liver injury. The composition provided by the present application can improve the activity of alcohol dehydrogenase / acetaldehyde dehydrogenase, reduce the content of MDA in tissues and increase the content of GSH, and reduce the content of triglyceride in liver cells, thereby comprehensively exerting the effect of protecting liver from alcohol, with high safety, small side effects, and wide sources of raw materials, and having important market value and development prospect.

The application discloses a kind of biological immobilized enzyme based on yeast surface display technology and the application of synthesis 2-phenylethanol, using yeast surface display technology, for the first time with Saccharomyces cerevisiae surface protein a-lectin as anchor protein, the key enzyme (transaminase, decarboxylase, ethanol dehydrogenase) involved in 2-PE producing Ehrlich pathway in Saccharomyces cerevisiae is separately or jointly carried out biological fixation, enhance substrate channel effect and proximity effect, in vitro one-step catalysis L-phenylalanine synthesis 2-PE, provide yield.The method for fixing multiple enzymes of Ehrlich pathway in the application can be used to reconfigure the biosynthesis pathway of other natural products in vitro, to provide feasible scheme for the rational design of cell factory.

The invention relates to the technical field of biology, and discloses a genetically engineered bacterium for high yield of 1, 4-butanediol and application of the genetically engineered bacterium. Escherichia coli is used as a chassis bacterium, a gene ldhA for coding lactic dehydrogenase, a gene adhE for coding ethanol dehydrogenase, a gene pflB for coding formate lyase, and a gene gabD and a gene yneI for coding succinate semialdehyde dehydrogenase are knocked out from a chassis bacterium genome, expression of a succinate semialdehyde dehydrogenase gene sucD and a 4-hydroxybutyrate dehydrogenase gene 4hbD is strengthened, a pdhR gene is knocked out, and a recombinant plasmid is obtained. The expression of the gene gltAR164L is enhanced, the gene mdh, the gene arcA, the gene poxB, the gene sdhAB and the gene ptsG are knocked out, and the expression of the glf gene and the ppc gene is enhanced; the method comprises the following steps of: transferring plasmids carrying a gene ald for coding aldehyde dehydrogenase, a gene cat2 for coding acyltransferase and a gene yqhD for coding alcohol dehydrogenase into a chassis bacterium, and carrying out metabolic transformation to obtain a genetically engineered bacterium which carries exogenous plasmids and has the capabilities of high yield and extracellular accumulation of 1, 4-BDO, so that the yield of 1, 4-BDO can be increased to 4.71 g / L.

1. The name of the design product: the main body of alcohol metabolism detector. 2. The use of the design product: alcohol metabolism detector and the main body of alcohol metabolism detector are used to understand the individual's alcohol metabolism ability by detecting alcohol dehydrogenase (ADH1B) and acetaldehyde dehydrogenase (ALDH2) double enzyme. 3. The design points of the design product: the shape of the main body of the alcohol metabolism detector. 4. The picture or photo that best indicates the design points: perspective view 1. 5. Other conditions that need to be explained: compared with perspective view 1, the cover at the top is rotated in the use state reference diagram. 6. Other conditions that need to be explained: the dotted line in the figure represents the unclaimed part of the design. And the five continuous circular structures in perspective view 1 and top view are indicator lights.

The invention discloses an ethanol dehydrogenase mutant, engineering bacteria and application in synthesis of a sinobamate intermediate, the ethanol dehydrogenase mutant is utilized to catalyze prochiral ketone (B) to synthesize chiral alcohol (A), the method is high in substrate concentration, simple and convenient in post-treatment step and more suitable for industrial large-scale production, and the problems that in the prior art, the substrate concentration is not high, and the yield is high are solved. Post-treatment is complicated, and the like.

The invention relates to the technical field of gene engineering, and discloses a recombinant Escherichia coli engineering strain for producing phenethyl alcohol by using L-phenylalanine as a substrate, the recombinant Escherichia coli engineering strain comprises a recombinant Escherichia coli engineering strain for producing phenethyl alcohol by using L-phenylalanine as a substrate, and the recombinant Escherichia coli contains a key gene capable of synthesizing phenethyl alcohol. Proteins coded by the key gene are a transaminase gene, a phenylpyruvate decarboxylase gene and an ethanol dehydrogenase gene. According to the invention, a recombinant plasmid containing a phenethyl alcohol biosynthetic pathway is constructed, the recombinant plasmid is transformed into Escherichia coli, and a recombinant engineering strain for improving the yield of phenethyl alcohol is screened out. The recombinant escherichia coli engineering strain contains transaminase KmARO8 derived from kluyveromyces marxianus, phenylpyruvate decarboxylase VvPDC1 derived from wine grapes and ethanol dehydrogenase AtADH1 derived from arabidopsis thaliana, the yield of phenethyl alcohol can be 7.29 g / L after fermentation culture of the recombinant strain, and the recombinant escherichia coli engineering strain has a good industrial prospect.

The invention discloses an application of a D. segeticola ethanol dehydrogenase (DsADH) gene in the regulation of the growth, the reproduction, the pathogenicity and the stress resistance of D. segeticola, and particularly discloses a D. segeticola ethanol dehydrogenase (DsADH) gene in the regulation of the growth, the reproduction, the pathogenicity and the stress resistance of D. segeticola. The DsADH gene disclosed by the invention is derived from a pathogenic bacterium D.segeticola, and a DsADH gene knockout gene segment is constructed and is introduced into a protoplast of the D.segeticola, so that a DsADH gene knockout mutant is obtained. Researches find that the DsADH gene plays an important role in regulating the growth and reproduction of D.segeticola and influencing osmotic stress and oxygen stress capabilities such as high salinity, and obviously reduces the pathogenicity of D.segeticola hyphae to tea tree leaves. Therefore, the DsADH can be used as a key gene for researching a disease pathogenic mechanism and a target of a bactericide, so that the bactericide for preventing and treating D.segeticola is researched and developed, or the DsADH is applied to gene function research of germs, a new variety for resisting plant diseases is favorably cultivated, and the DsADH has a wide application prospect in plant fungal disease research.

The invention provides a compound peptide beverage capable of dispelling effects of alcohol and protecting liver as well as a preparation method and application thereof. The compound peptide beverage is prepared from hovenia dulcis thunb oligopeptide, walnut peptide, antioxidant peptide, energy release auxiliary peptide, ethanol dehydrogenase activating peptide, silibinin-serine chelate, an electrolyte poising agent, alginic acid oligosaccharide and purified water. Wherein the antioxidant peptide is prepared from cordyceps militaris, sea-buckthorn and sunflower disc through mixed enzymolysis, the energy release auxiliary peptide is prepared from carnosine and arginine succinic acid peptide through microbial fermentation, and the ethanol dehydrogenase activating peptide is prepared from marine shellfish collagen through fermentation. The composite peptide beverage shows excellent functions of dispelling the effects of alcohol and protecting the liver, and can be used for developing medicinal products or functional foods for dispelling the effects of alcohol and protecting the liver. Through precise compatibility and an innovative process technology, the product constructs a unique solution for dispelling the effects of alcohol and protecting the liver, and has outstanding performance in the aspects of improving the alcohol metabolism efficiency and relieving liver injury.

The application discloses a high-temperature inducible promoter and application thereof, and belongs to the field of genetic engineering and fermentation engineering. Acetobacter pasteurianus A high-temperature inducible promoter P icd is screened from a bacillus pabuli (Olsenella ) and has a nucleotide sequence as shown in SEQ ID NO. 1. The application further constructs a recombinant expression vector pBBR1MCS2-icd containing the promoter and a recombinant bacterium expressing an ethanol dehydrogenase gene AdhP. The recombinant strain ApAdhP(icd) using the promoter to regulate the expression of the ethanol dehydrogenase can not only reduce the metabolic burden of the cell and maintain a good growth trend under high-temperature fermentation conditions, but also can reach an acetic acid final yield of 52.65 g / L, which is increased by about 31.5% compared with a wild strain. The application effectively solves the problems of the decrease of acetic acid production rate and yield under high-temperature environment in summer in traditional acetic acid fermentation.

The invention provides an anti-alcohol functional water based on liver enzyme activation, and relates to the technical field of functional water. The hangover alleviating functional water based on liver enzyme activation is prepared from radix puerariae isoflavone aglycone, globe artichoke water-soluble polysaccharide, emblic leafflower fruit procyanidine oligomer, an electrolyte combination, a pH buffer system, hovenia dulcis thunb, fructus gardeniae, beta-nicotinamide mononucleotide, hydrogen-rich water, vitamin C, vitamin E and curcumin, and the electrolyte combination is prepared from sodium chloride, potassium chloride and sodium bicarbonate. Alcohol dehydrogenase and acetaldehyde dehydrogenase in the liver can be activated through the radix puerariae isoflavone aglycone, the globe artichoke water-soluble polysaccharide, the emblic leafflower fruit proanthocyanidin oligomer and the like, so that decomposition and metabolism of alcohol are accelerated, the retention time of the alcohol in the body can be remarkably shortened by activating the enzymes and dispelling drunkenness, and the symptoms of drunkenness and hangover are reduced.

The present invention relates to a recombinant halomonas for the production of 1, 4-butanediol (1, 4-BDO), which overexpresses a 4-hydroxybutyryl-CoA reductase (adhE) and an ethanol dehydrogenase (yqhD) from E. coli (E. coli), in particular to a recombinant halomonas for the production of 1, 4-butanediol (1, 4-BDO). In order to further improve the production efficiency, glucose-6-phosphate dehydrogenase (zwf) is also overexpressed. The synthesis of the 1, 4-BDO is realized in the halomonas for the first time, the production process is simple, the cost is low, and the application prospect is wide.

The invention relates to the technical field of medical preparations, and discloses application of HMB (beta-hydroxy-beta-methylbutyric acid) or an HMB-containing substance in preparation of a medicine for preventing and / or relieving alcohol hangover and / or relieving acute alcoholic liver injury. The substance containing HMB comprises calcium beta-hydroxy-beta-methylbutyrate (CaHMB), the CaHMB can significantly improve the tolerance of acute alcohol exposure and improve damaged exercise coordination, and the CaHMB can reduce the content of ethanol in serum and accelerate the change process of acetaldehyde in the serum; meanwhile, CaHMB can enhance the activity of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH2) in the liver and accelerate the alcohol metabolism process; besides, the CaHMB can reduce the levels of ALT and AST in serum, relieve liver inflammation caused by alcohol, effectively relieve liver injury and achieve the effects of dispelling the effects of alcohol and protecting the liver.

1. The name of this design product: the main body of the alcohol metabolism capacity detector. 2. Purpose of the product of this design: The alcohol metabolism capacity detector and the main body of the alcohol metabolism capacity detector are used to understand an individual's alcohol metabolism capacity through the detection of the two enzymes of alcohol dehydrogenase (ADH1B) and acetaldehyde dehydrogenase (ALDH2). 3. The key design point of this design product lies in the shape of the main body of the alcohol metabolism capacity detector. 4. The picture or photo that best illustrates the key points of the design: Design 1 Stereoscopic Figure 1. 5. Designate Design 1 as the base design. 6. Other circumstances requiring clarification Other explanations: The dotted lines in the figures indicate portions of the design for which protection is not claimed. The five continuously distributed circular structures within the transparent structure in the design 2 stereoscopic diagram 1, the design 2 top view and the design 2 usage status reference diagram are indicator lights.

The invention belongs to the technical field of medicines, and particularly relates to lactobacillus rhamnosus for relieving acute alcoholic liver injury as well as a metagen and application of the lactobacillus rhamnosus. The lactobacillus rhamnosus L11-4 strain is preserved in the China Center for Type Culture Collection on August 18, 2025, and the preservation number of the lactobacillus rhamnosus L11-4 strain is CCTCC (China Center for Type Culture Collection) NO: M 20251834. The strain and the metagen thereof can significantly improve pathological symptoms of mice with acute alcoholic liver injury, and have the effects of reducing the levels of ALT, AST, TC, TG and LDL-C in serum of the mice with acute alcoholic liver injury, increasing the level of HDL-C, and regulating abnormal blood lipid level of the mice caused by acute alcohol; the activity of antioxidant enzymes in the liver is improved, and the lipid peroxidation level is reduced; in addition, the ethanol dehydrogenase activity of the liver can be improved, and the ethanol metabolism capability is enhanced. Therefore, the strain and the metagen thereof have wide application prospects in prevention or treatment of acute alcoholic liver injury.

The invention discloses a sea cucumber-sourced small molecule peptide with effects of dispelling effects of alcohol and protecting liver and application thereof, and belongs to the technical field of bioactive peptides. The sea cucumber source small molecule peptide contains a key active component sea cucumber heptapeptide (the amino acid sequence is QAGFPGR). The preparation method comprises the following steps: by taking fresh frozen East China Sea black sea cucumbers as raw materials, cooking, shearing and homogenizing, performing enzymolysis with compound protease, filtering, performing membrane separation, concentrating and drying and the like. The sea cucumber-sourced small molecule peptide and sea cucumber heptapeptide can significantly improve the activity of alcohol metabolism key enzymes (ethanol dehydrogenase and acetaldehyde dehydrogenase), reduce the level of liver injury markers (glutamic-pyruvic transaminase and glutamic oxalacetic transaminase), prolong the alcohol tolerance time, shorten the drunkenness duration, improve the reduction of exercise ability caused by alcohol, and improve the activity of liver injury. And the beverage has definite hangover-alleviating and liver-protecting effects.

The invention belongs to the technical field of food, and relates to a honeysuckle-spirulina compound composition and a preparation method thereof, which can be applied to preparation of hangover alleviating health care products, functional beverages or pharmaceutical compositions for adjuvant therapy of alcoholic liver injury. The preparation method comprises the following steps: (1) weighing honeysuckle powder, adding 15-25 times volume of distilled water into 1-10 parts of honeysuckle powder, stirring and extracting for 160-200 minutes in a water bath at 70-80 DEG C in a dark place, and carrying out solid-liquid separation to obtain a honeysuckle extracting solution; (2) weighing 5-20 parts of spirulina platensis powder, and preparing a spirulina platensis extraction solution according to the method in the step (1); (3) mixing the obtained honeysuckle flower extracting solution with the spirulina platensis extracting solution; wherein the matching effect of 5 parts of honeysuckle flower powder and 10 parts of spirulina powder is optimal. The compound composition can solve the problem that the honeysuckle water extract is unstable, the stability index (TSI) within 6 hours is obviously lower than that of a single raw material group, and the total phenol retention rate is higher after the gastrointestinal environment is simulated; the natural anti-alcoholism liver-protecting food has the advantages that the anti-alcoholism liver-protecting food has a synergistic anti-alcoholism liver-protecting effect, the survival rate of HepG2 cells damaged by alcohol is obviously increased, the activities of ethanol dehydrogenase and acetaldehyde dehydrogenase are improved, the activities of glutamic-pyruvic transaminase and glutamic oxalacetic transaminase are reduced, meanwhile, the scavenging rates of DPPH, ABTS and hydroxyl radicals are excellent, and a new direction is provided for development of natural anti-alcoholism liver-protecting food.

The invention provides a phenolic compound with an effect of accelerating alcohol metabolism and a preparation method of the phenolic compound. The structural formula of the polyphenol compound 1-(2-hydroxyl, 4-O-glucosylphenyl)-2-(4-hydroxyphenyl) ethanone is as shown in formula (I), and the polyphenol compound 1-(2-hydroxyl, 4-O-glucosylphenyl)-2-(4-hydroxyphenyl) ethanone is separated from radix puerariae. In-vitro pharmacological experiments prove that the compound 1-(2-hydroxyl, 4-O-glucosylphenyl)-2-(4-hydroxyphenyl) ethanone has good acetylcholin esterase inhibitory activity, and the EC50 value of the compound 1-(2-hydroxyl, 4-O-glucosylphenyl)-2-(4-hydroxyphenyl) ethanone is 1.41 + / -0.12 mM, which indicates that the compound 1-(2-hydroxyl, 4-O-glucosylphenyl)-2-( The polyphenol compound 1-(2-hydroxyl, 4-O-glucosylphenyl)-2-(4-hydroxyphenyl) ethanone is expected to be used as a lead compound to develop a novel alcohol effect dispelling and liver protecting medicine.

This invention relates to the field of biological products, and particularly to the preparation and application of hangover relief products. This invention provides an alcohol dehydrogenase mutant, an aldehyde dehydrogenase mutant, an expression vector, a combination of recombinant yeast strains, and yeast single-cell oral capsules. By cloning the alcohol dehydrogenase and aldehyde dehydrogenase genes into the *Saccharomyces cerevisiae* expression plasmid pYX212, and then transforming the *Saccharomyces cerevisiae* into the *Saccharomyces cerevisiae*, engineered yeast is obtained. Utilizing the protective effect of yeast cells, oral administration is achieved, effectively reducing the destruction of recombinant enzymes by gastric juice. The yeast single-cell oral capsules can accelerate alcohol metabolism after daily drinking and promote hepatocyte regeneration; they are convenient and quick to take and have significant effects.

The present application relates to a kind of pentosus PP90 for preventing and treating alcoholic nerve injury and its application, wherein pentosus PP90 is preserved in China Microbial Culture Collection Center, and the preservation number is CGMCC NO.32034.The fermentation product of the pentosus PP90 can degrade the alcoholicity of alcohol, can produce ethanol dehydrogenase and acetaldehyde dehydrogenase, can improve the viability of nerve cell, can regulate the expression of cytokine, can inhibit the activation of astrocyte, can regulate the expression of brain-derived neurotrophic factor, can improve the level of disturbed neurotransmitter, and can be used for preparing product for preventing and treating alcoholic nerve injury or improving alcoholic nerve injury.

The invention relates to the fields of gene engineering, enzyme engineering and bioinformatics, and particularly discloses application of glycine free radical enzyme in preparation of propylene glycol. Transforming a glycolytic pathway of escherichia coli: knocking out phosphofructokinase, acetaldehyde dehydrogenase, lactic dehydrogenase and ethanol dehydrogenase of the strain; according to the present invention, the following genes driven by the strong promoter, such as glycerol dehydrogenase, fructose-6-phosphate aldolase, mannitol-1-phosphate dehydrogenase, and dihydroxyacetone kinase, glycine free radical enzyme and free radical activating enzyme of citrobacter freundii, are integrated in the genome so as to enhance the metabolic pathway for synthesizing 1, 2-propylene glycol, and finally obtain the genetically engineered bacterium, wherein the genes comprise glycerol dehydrogenase, fructose-6-phosphate aldolase, mannitol-1-phosphate dehydrogenase, dihydroxyacetone kinase, glycine free radical enzyme and free radical activating enzyme of citrobacter freundii. The R-1, 2-propylene glycol produced by using the genetically engineered bacterium through a biological method can be applied to the fields of medicines, cosmetics, foods and the like, and high-added-value products are developed to meet the requirements of different fields on the R-1, 2-propylene glycol.

Alcohol dehydrogenase 1 enzymes, aldehyde dehydrogenase 2 enzymes, nucleic acids encoding alcohol dehydrogenase 1 enzymes and aldehyde dehydrogenase 2 enzymes, lipid nanoparticles, lipid nanoparticles comprising nucleic acids encapsulated therein, pharmaceutical compositions comprising lipid nanoparticles which comprise nucleic acids encapsulated therein are useful for treating alcohol-related conditions, such as alcohol poisoning.