55 results about "Ethanol dehydrogenase" patented technology

The invention discloses a construction method of an escherichia coli mutant for producing succinic acid by fermentation of a synthetic medium. The method comprises the following steps: firstly, knocking out a lactic dehydrogenase gene ldhA, a pyruvate formate lyase gene pflB, a ptsG gene responsible for encoding a phosphotransferase system EIIBC protein, an ethanol dehydrogenase gene adhE, an acetokinase-phosphate transacetylase gene ackA-pta, and a ptsG gene responsible for encoding a phosphotransferase system EIIBC protein in escherichia coli; a phosphoenolpyruvate carboxykinase gene pck from bacillus subtilis is integrated at an SS9 safety site of a strain to obtain escherichia coli ESC6 with high succinic acid yield; and mutating one or more loci in one or more genes of a glucose-transcriptional inhibition factor gene mlc, a nitrate response regulatory factor gene narL and a cyclic adenylate receptor protein gene crp to obtain the escherichia coli mutant capable of producing succinic acid by fermentation of a synthetic culture medium, wherein the one or more loci in one or more genes of the glucose-transcriptional inhibition factor gene mlc, the nitrate response regulatory factor gene narL and the cyclic adenylate receptor protein gene crp are mutated. The strain can be fermented in a synthetic medium to produce succinic acid, so that the fermentation cost is greatly reduced, and the strain has a very wide application prospect.

The invention relates to the technical field of genetic engineering, in particular to a recombinant microorganism and application thereof in preparation of glycine. The application comprises the following steps: producing glycine by adopting the recombinant microorganism; the recombinant microorganism comprises an enzyme combination and / or a nucleic acid molecule combination. The enzyme combination comprises threonine aldolase, ethanol dehydrogenase and isopropanol dehydrogenase; the threonine aldolase comprises an amino acid sequence as shown in SEQ ID NO. 1. The nucleic acid molecule combination is used for encoding the enzyme combination. According to the invention, threonine aldolase, ethanol dehydrogenase and isopropanol dehydrogenase are converted into microorganisms, and the constructed recombinant microorganisms can be used for decomposing threonine to produce glycine; and a specific system formed by specific isopropanol dehydrogenase, ethanol dehydrogenase and isopropanol dehydrogenase can also have a relatively high conversion rate under the condition of no addition of pyridoxal phosphate (PLP), and has important significance and economic value.

The invention relates to the technical field of gene engineering, in particular to an ethanol response type gene engineering alcohol-dissolving probiotic as well as a construction method and application thereof. According to the probiotics provided by the invention, an ethanol-responsive regulation mechanism is innovatively introduced, so that the strain can specifically induce high-efficiency expression of human ethanol dehydrogenase 1B (hADH1B) and human acetaldehyde dehydrogenase 2 (hALDH2) when sensing the existence of ethanol in vivo, thereby realizing on-demand and high-efficiency decomposition of ethanol and toxic metabolite acetaldehyde thereof. Compared with continuous expression of traditional engineering bacteria, the intelligent regulation and control mode can better simulate the natural metabolic rhythm of a human body, solves the problem of bacterial metabolism, reduces the metabolic pressure of thalli while improving the hangover alleviating efficiency, and is expected to achieve a better hangover alleviating effect and higher biological safety.

This invention relates to the field of functional food technology, and more particularly to the application of safflower honey, or safflower honey extract, in enhancing the activity of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH). The invention has found that safflower honey, or safflower honey extract, can effectively enhance ADH and ALDH activity, thereby promoting alcohol metabolism and achieving a hangover-relieving effect. Further research on the extract revealed that a mixture of syringaldehyde, riboflavin, lutein, luciferin, and three p-coumaroyl spermidine plays a key role in enhancing ADH and ALDH activity, thus providing an application of this mixture in hangover relief. The application of safflower honey and its extract provided by this invention has high application value for developing foods and health products with hangover-relieving effects.

The present application provides a kind of kudzu vine cranberry composition for protecting liver from alcohol, which contains the following ingredients by weight: 30-50 parts of extract of Hovenia dulcis Thunb, 30-50 parts of mixed extract, 20-40 parts of extract of Pueraria lobata, 10-20 parts of extract of Poria cocos, 10-20 parts of extract of Glycyrrhiza uralensis, 0.05-0.1 parts of vitamin C; the raw materials of the mixed extract are mixed by cranberry and Fructus Lycii in a mass ratio of (1-2):(1-2). The present application further provides formulations and food containing the composition. The present application further protects the use of the composition or formulation in preparing health food or medicine with auxiliary protective effect on chemical liver injury. The composition provided by the present application can improve the activity of alcohol dehydrogenase / acetaldehyde dehydrogenase, reduce the content of MDA in tissues and increase the content of GSH, and reduce the content of triglyceride in liver cells, thereby comprehensively exerting the effect of protecting liver from alcohol, with high safety, small side effects, and wide sources of raw materials, and having important market value and development prospect.

The application discloses a kind of biological immobilized enzyme based on yeast surface display technology and the application of synthesis 2-phenylethanol, using yeast surface display technology, for the first time with Saccharomyces cerevisiae surface protein a-lectin as anchor protein, the key enzyme (transaminase, decarboxylase, ethanol dehydrogenase) involved in 2-PE producing Ehrlich pathway in Saccharomyces cerevisiae is separately or jointly carried out biological fixation, enhance substrate channel effect and proximity effect, in vitro one-step catalysis L-phenylalanine synthesis 2-PE, provide yield.The method for fixing multiple enzymes of Ehrlich pathway in the application can be used to reconfigure the biosynthesis pathway of other natural products in vitro, to provide feasible scheme for the rational design of cell factory.

The invention discloses an ethanol dehydrogenase mutant, engineering bacteria and application in synthesis of a sinobamate intermediate, the ethanol dehydrogenase mutant is utilized to catalyze prochiral ketone (B) to synthesize chiral alcohol (A), the method is high in substrate concentration, simple and convenient in post-treatment step and more suitable for industrial large-scale production, and the problems that in the prior art, the substrate concentration is not high, and the yield is high are solved. Post-treatment is complicated, and the like.

The application discloses a high-temperature inducible promoter and application thereof, and belongs to the field of genetic engineering and fermentation engineering. Acetobacter pasteurianus A high-temperature inducible promoter P icd is screened from a bacillus pabuli (Olsenella ) and has a nucleotide sequence as shown in SEQ ID NO. 1. The application further constructs a recombinant expression vector pBBR1MCS2-icd containing the promoter and a recombinant bacterium expressing an ethanol dehydrogenase gene AdhP. The recombinant strain ApAdhP(icd) using the promoter to regulate the expression of the ethanol dehydrogenase can not only reduce the metabolic burden of the cell and maintain a good growth trend under high-temperature fermentation conditions, but also can reach an acetic acid final yield of 52.65 g / L, which is increased by about 31.5% compared with a wild strain. The application effectively solves the problems of the decrease of acetic acid production rate and yield under high-temperature environment in summer in traditional acetic acid fermentation.

The invention provides an anti-alcohol functional water based on liver enzyme activation, and relates to the technical field of functional water. The hangover alleviating functional water based on liver enzyme activation is prepared from radix puerariae isoflavone aglycone, globe artichoke water-soluble polysaccharide, emblic leafflower fruit procyanidine oligomer, an electrolyte combination, a pH buffer system, hovenia dulcis thunb, fructus gardeniae, beta-nicotinamide mononucleotide, hydrogen-rich water, vitamin C, vitamin E and curcumin, and the electrolyte combination is prepared from sodium chloride, potassium chloride and sodium bicarbonate. Alcohol dehydrogenase and acetaldehyde dehydrogenase in the liver can be activated through the radix puerariae isoflavone aglycone, the globe artichoke water-soluble polysaccharide, the emblic leafflower fruit proanthocyanidin oligomer and the like, so that decomposition and metabolism of alcohol are accelerated, the retention time of the alcohol in the body can be remarkably shortened by activating the enzymes and dispelling drunkenness, and the symptoms of drunkenness and hangover are reduced.

The present invention relates to a recombinant halomonas for the production of 1, 4-butanediol (1, 4-BDO), which overexpresses a 4-hydroxybutyryl-CoA reductase (adhE) and an ethanol dehydrogenase (yqhD) from E. coli (E. coli), in particular to a recombinant halomonas for the production of 1, 4-butanediol (1, 4-BDO). In order to further improve the production efficiency, glucose-6-phosphate dehydrogenase (zwf) is also overexpressed. The synthesis of the 1, 4-BDO is realized in the halomonas for the first time, the production process is simple, the cost is low, and the application prospect is wide.

The invention relates to the technical field of medical preparations, and discloses application of HMB (beta-hydroxy-beta-methylbutyric acid) or an HMB-containing substance in preparation of a medicine for preventing and / or relieving alcohol hangover and / or relieving acute alcoholic liver injury. The substance containing HMB comprises calcium beta-hydroxy-beta-methylbutyrate (CaHMB), the CaHMB can significantly improve the tolerance of acute alcohol exposure and improve damaged exercise coordination, and the CaHMB can reduce the content of ethanol in serum and accelerate the change process of acetaldehyde in the serum; meanwhile, CaHMB can enhance the activity of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH2) in the liver and accelerate the alcohol metabolism process; besides, the CaHMB can reduce the levels of ALT and AST in serum, relieve liver inflammation caused by alcohol, effectively relieve liver injury and achieve the effects of dispelling the effects of alcohol and protecting the liver.

The invention belongs to the technical field of medicines, and particularly relates to lactobacillus rhamnosus for relieving acute alcoholic liver injury as well as a metagen and application of the lactobacillus rhamnosus. The lactobacillus rhamnosus L11-4 strain is preserved in the China Center for Type Culture Collection on August 18, 2025, and the preservation number of the lactobacillus rhamnosus L11-4 strain is CCTCC (China Center for Type Culture Collection) NO: M 20251834. The strain and the metagen thereof can significantly improve pathological symptoms of mice with acute alcoholic liver injury, and have the effects of reducing the levels of ALT, AST, TC, TG and LDL-C in serum of the mice with acute alcoholic liver injury, increasing the level of HDL-C, and regulating abnormal blood lipid level of the mice caused by acute alcohol; the activity of antioxidant enzymes in the liver is improved, and the lipid peroxidation level is reduced; in addition, the ethanol dehydrogenase activity of the liver can be improved, and the ethanol metabolism capability is enhanced. Therefore, the strain and the metagen thereof have wide application prospects in prevention or treatment of acute alcoholic liver injury.

The invention discloses a sea cucumber-sourced small molecule peptide with effects of dispelling effects of alcohol and protecting liver and application thereof, and belongs to the technical field of bioactive peptides. The sea cucumber source small molecule peptide contains a key active component sea cucumber heptapeptide (the amino acid sequence is QAGFPGR). The preparation method comprises the following steps: by taking fresh frozen East China Sea black sea cucumbers as raw materials, cooking, shearing and homogenizing, performing enzymolysis with compound protease, filtering, performing membrane separation, concentrating and drying and the like. The sea cucumber-sourced small molecule peptide and sea cucumber heptapeptide can significantly improve the activity of alcohol metabolism key enzymes (ethanol dehydrogenase and acetaldehyde dehydrogenase), reduce the level of liver injury markers (glutamic-pyruvic transaminase and glutamic oxalacetic transaminase), prolong the alcohol tolerance time, shorten the drunkenness duration, improve the reduction of exercise ability caused by alcohol, and improve the activity of liver injury. And the beverage has definite hangover-alleviating and liver-protecting effects.

The invention belongs to the technical field of food, and relates to a honeysuckle-spirulina compound composition and a preparation method thereof, which can be applied to preparation of hangover alleviating health care products, functional beverages or pharmaceutical compositions for adjuvant therapy of alcoholic liver injury. The preparation method comprises the following steps: (1) weighing honeysuckle powder, adding 15-25 times volume of distilled water into 1-10 parts of honeysuckle powder, stirring and extracting for 160-200 minutes in a water bath at 70-80 DEG C in a dark place, and carrying out solid-liquid separation to obtain a honeysuckle extracting solution; (2) weighing 5-20 parts of spirulina platensis powder, and preparing a spirulina platensis extraction solution according to the method in the step (1); (3) mixing the obtained honeysuckle flower extracting solution with the spirulina platensis extracting solution; wherein the matching effect of 5 parts of honeysuckle flower powder and 10 parts of spirulina powder is optimal. The compound composition can solve the problem that the honeysuckle water extract is unstable, the stability index (TSI) within 6 hours is obviously lower than that of a single raw material group, and the total phenol retention rate is higher after the gastrointestinal environment is simulated; the natural anti-alcoholism liver-protecting food has the advantages that the anti-alcoholism liver-protecting food has a synergistic anti-alcoholism liver-protecting effect, the survival rate of HepG2 cells damaged by alcohol is obviously increased, the activities of ethanol dehydrogenase and acetaldehyde dehydrogenase are improved, the activities of glutamic-pyruvic transaminase and glutamic oxalacetic transaminase are reduced, meanwhile, the scavenging rates of DPPH, ABTS and hydroxyl radicals are excellent, and a new direction is provided for development of natural anti-alcoholism liver-protecting food.

The invention provides a phenolic compound with an effect of accelerating alcohol metabolism and a preparation method of the phenolic compound. The structural formula of the polyphenol compound 1-(2-hydroxyl, 4-O-glucosylphenyl)-2-(4-hydroxyphenyl) ethanone is as shown in formula (I), and the polyphenol compound 1-(2-hydroxyl, 4-O-glucosylphenyl)-2-(4-hydroxyphenyl) ethanone is separated from radix puerariae. In-vitro pharmacological experiments prove that the compound 1-(2-hydroxyl, 4-O-glucosylphenyl)-2-(4-hydroxyphenyl) ethanone has good acetylcholin esterase inhibitory activity, and the EC50 value of the compound 1-(2-hydroxyl, 4-O-glucosylphenyl)-2-(4-hydroxyphenyl) ethanone is 1.41 + / -0.12 mM, which indicates that the compound 1-(2-hydroxyl, 4-O-glucosylphenyl)-2-( The polyphenol compound 1-(2-hydroxyl, 4-O-glucosylphenyl)-2-(4-hydroxyphenyl) ethanone is expected to be used as a lead compound to develop a novel alcohol effect dispelling and liver protecting medicine.

This invention relates to the field of biological products, and particularly to the preparation and application of hangover relief products. This invention provides an alcohol dehydrogenase mutant, an aldehyde dehydrogenase mutant, an expression vector, a combination of recombinant yeast strains, and yeast single-cell oral capsules. By cloning the alcohol dehydrogenase and aldehyde dehydrogenase genes into the *Saccharomyces cerevisiae* expression plasmid pYX212, and then transforming the *Saccharomyces cerevisiae* into the *Saccharomyces cerevisiae*, engineered yeast is obtained. Utilizing the protective effect of yeast cells, oral administration is achieved, effectively reducing the destruction of recombinant enzymes by gastric juice. The yeast single-cell oral capsules can accelerate alcohol metabolism after daily drinking and promote hepatocyte regeneration; they are convenient and quick to take and have significant effects.

The present application relates to a kind of pentosus PP90 for preventing and treating alcoholic nerve injury and its application, wherein pentosus PP90 is preserved in China Microbial Culture Collection Center, and the preservation number is CGMCC NO.32034.The fermentation product of the pentosus PP90 can degrade the alcoholicity of alcohol, can produce ethanol dehydrogenase and acetaldehyde dehydrogenase, can improve the viability of nerve cell, can regulate the expression of cytokine, can inhibit the activation of astrocyte, can regulate the expression of brain-derived neurotrophic factor, can improve the level of disturbed neurotransmitter, and can be used for preparing product for preventing and treating alcoholic nerve injury or improving alcoholic nerve injury.

The invention relates to the fields of gene engineering, enzyme engineering and bioinformatics, and particularly discloses application of glycine free radical enzyme in preparation of propylene glycol. Transforming a glycolytic pathway of escherichia coli: knocking out phosphofructokinase, acetaldehyde dehydrogenase, lactic dehydrogenase and ethanol dehydrogenase of the strain; according to the present invention, the following genes driven by the strong promoter, such as glycerol dehydrogenase, fructose-6-phosphate aldolase, mannitol-1-phosphate dehydrogenase, and dihydroxyacetone kinase, glycine free radical enzyme and free radical activating enzyme of citrobacter freundii, are integrated in the genome so as to enhance the metabolic pathway for synthesizing 1, 2-propylene glycol, and finally obtain the genetically engineered bacterium, wherein the genes comprise glycerol dehydrogenase, fructose-6-phosphate aldolase, mannitol-1-phosphate dehydrogenase, dihydroxyacetone kinase, glycine free radical enzyme and free radical activating enzyme of citrobacter freundii. The R-1, 2-propylene glycol produced by using the genetically engineered bacterium through a biological method can be applied to the fields of medicines, cosmetics, foods and the like, and high-added-value products are developed to meet the requirements of different fields on the R-1, 2-propylene glycol.

Alcohol dehydrogenase 1 enzymes, aldehyde dehydrogenase 2 enzymes, nucleic acids encoding alcohol dehydrogenase 1 enzymes and aldehyde dehydrogenase 2 enzymes, lipid nanoparticles, lipid nanoparticles comprising nucleic acids encapsulated therein, pharmaceutical compositions comprising lipid nanoparticles which comprise nucleic acids encapsulated therein are useful for treating alcohol-related conditions, such as alcohol poisoning.

The invention relates to collagen hydrogel, a preparation method thereof and application of the collagen hydrogel in delivery of hangover-alleviating active enzyme. According to the collagen hydrogel provided by the invention, the collagen-based hydrogel is combined with the micro-scale grid and the artificial villus structure, so that the effects of prolonging the retention time in the stomach, enhancing the mechanical toughness and improving the compressive strength are achieved under physiological conditions, and the long-standing problem of balancing between the mechanical stability and the catalytic efficiency is solved; meanwhile, the excellent biocompatibility is realized. Taking hangover alleviating application as an example, the acetaldehyde removal efficiency of the system is improved by 30 times in a simulated stomach environment by fixing a double-enzyme cascade system of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH), and good quick hangover alleviating application potential is shown.

This invention provides a fermenting *Lactobacillus mucinus* strain that can accelerate alcohol metabolism and improve hangover symptoms, along with its applications. This strain exhibits high safety, lacking resistance genes and pathogenic virulence genes, and demonstrates strong tolerance to bile salts, artificial gastric juice, and artificial intestinal juice. The bacterium possesses strong antioxidant capabilities, exhibiting potent scavenging abilities against ABTS, DPPH, and hydroxyl radicals. This fermenting *Lactobacillus mucinus* can increase the alcohol dehydrogenase content in a mouse model of alcoholic liver disease, demonstrating significant application value in antioxidation, alcohol detoxification, and hangover prevention. Furthermore, this bacterium can produce melatonin and convert NR to niacin.

This invention discloses an alcohol dehydrogenase, recombinant genetically engineered bacteria, and their application in the asymmetric reduction of prochiral carbonyl compounds. The alcohol dehydrogenase exhibits excellent biological properties, achieving heterologous expression in engineered *E. coli* bacteria. When using whole cells as a catalyst, it can directly utilize intracellular coenzymes, achieving zero coenzyme addition. This enzyme possesses advantages such as high activity, high stereoselectivity, and a broad substrate spectrum, enabling green and efficient catalysis of the conversion of prochiral ketone compounds into corresponding chiral alcohols. This solves the problems of low conversion rate, low yield, low ee value, complex reaction process, and numerous byproducts in traditional chemical methods. The alcohol dehydrogenase of this invention exhibits excellent tolerance to isopropanol, allowing isopropanol to be used as a hydrogen source for coenzyme cycling. Furthermore, the byproducts of the enzyme-catalyzed reaction—acetone and the remaining isopropanol—can be separated and recovered through technical means, significantly reducing production costs.

The application provides a polypeptide with activated ethanol dehydrogenase activity and application thereof, and relates to the technical field of active polypeptides.The application provides a polypeptide with activated ethanol dehydrogenase activity, wherein the amino acid sequence of the polypeptide is shown as SEQ ID No.1.The polypeptide can activate the activity of ethanol dehydrogenase, stably combine with ADH, improve the catalytic efficiency of ADH on ethanol, reduce the ethanol content in the serum of a mouse after drinking, effectively exert the effects of relieving alcohol, effectively relieve the adverse reactions of a drunk person such as hangover, fatigue and dizziness, and can be used in products for relieving alcohol and protecting liver.

The application discloses a genetically engineered bacterium for improving the yield of mevalonic acid as well as a construction method and application thereof, and belongs to the technical field of genetic engineering. In order to solve the technical problems of low carbon atom economy and limited theoretical yield of MVA biosynthesis technology, the application takes Escherichia coli as a starting strain, overexpresses a bifunctional acetaldehyde-ethanol dehydrogenase mutant AdhE A267T / E568K gene adhE mut , an acetyl-CoA acyltransferase / HMG-CoA reductase gene mvaE , an HMG-CoA synthetase gene mvaS , and introduces a tetracycline-induced CRISPRi system to inhibit the expression of an orotate-5'-phosphate decarboxylase gene pyrF , so that the carbon flux is led from the growth of the strain to the synthesis of mevalonic acid. The genetically engineered bacterium provided by the application can produce mevalonic acid by fermentation with ethanol as a carbon source, and the yield reaches 0.89 g / g. The application provides a new idea for improving the carbon atom economy of mevalonic acid biological manufacturing.

The invention belongs to the technical field of enzyme engineering, and particularly relates to immobilized ethanol dehydrogenase as well as a preparation method and application thereof. The immobilized ethanol dehydrogenase is prepared by taking a magnetic metal organic framework material Fe3O4 (at) MIL-101 (Fe) as a carrier, and carrying out covalent cross-linking and ethanol dehydrogenase adsorption on the magnetic metal organic framework material Fe3O4 (at) MIL-101 (Fe), so as to obtain the immobilized ethanol dehydrogenase. The prepared immobilized ethanol dehydrogenase is combined with a liquid chromatography-mass spectrometry (LC-MS) technology, and an efficient platform is provided for screening of ethanol dehydrogenase inhibitors in traditional Chinese medicine. Compared with a traditional free enzyme screening method, the technology has the remarkable advantages that the analysis time is short, the screening flux is greatly improved, and the single-time operation cost is greatly reduced due to repeated use of the immobilized enzyme.

The invention discloses klebsiella pneumoniae based on CRISPR (clustered regularly interspaced short palindromic repeats) / cas12a and detection of an ethanol dehydrogenase gene of the klebsiella pneumoniae. The invention provides a kit for detecting klebsiella pneumoniae and an ethanol dehydrogenase gene thereof. The kit comprises the following components: 1) a primer group for amplifying a gene rcsA; 2) a primer group for amplifying the gene adh; 3) a Cas12a protein, a crRNA combined with a gene rcsA and a crRNA combined with a gene adh; a specific and conservative capsular polysaccharide synthesis regulatory factor rcsA is selected as a target sequence for identifying klebsiella pneumoniae, and an ethanol dehydrogenase gene adh is selected as a target sequence for identifying ethanol production. Meanwhile, the detection conditions of the platform are optimized, and the sensitivity and specificity of the platform are determined. The invention develops a double-target detection kit based on CRISPR / Cas12a, and realizes high-sensitivity and high-specificity rapid identification of klebsiella pneumoniae and ethanol production capacity thereof by simultaneously targeting rcsA gene and adh gene.

This invention provides a two-phase aqueous extraction apparatus and a method for simultaneously extracting alcohol dehydrogenase and heparin from pig liver, belonging to the field of chemical manufacturing technology. The two-phase aqueous extraction apparatus includes two storage cylinders; each storage cylinder has an independently mounted rotating shaft; a driving wheel is fixedly mounted on each rotating shaft; a first driven wheel is mounted on the left side of the driving wheel; a first horizontal shaft is fixedly mounted at the center of the first driven wheel; the first horizontal shaft is rotatably mounted on the inner wall of the storage cylinder; a first stirring rod is evenly arranged on the outer wall of the first horizontal shaft; a second driven wheel is engaged on the right side of the driving wheel; a second horizontal shaft is fixedly mounted at the center of the second driven wheel. Using this apparatus to extract from pig liver is not only efficient, simple, and environmentally friendly, but also allows for the simultaneous extraction of highly active alcohol dehydrogenase and high-purity heparin. This invention provides new ideas for related fields such as the food and pharmaceutical industries.

The invention relates to the technical field of molecular genetic breeding, in particular to application of a mung bean ethanol dehydrogenase coding gene VrADH in resisting mung bean yellow mosaic disease. The mung bean ethanol dehydrogenase encoding gene VrADH can enhance the resistance to MYMIV, the mung bean VrADH is subjected to up-regulation expression in leaves after being induced by the MYMIV, meanwhile, it is found through tobacco instantaneous conversion verification that the VrADH can positively regulate the resistance to the MYMIV, and a material, carrying the VrADH, in a mung bean material created through hybridization shows the resistance to the MYMIV; therefore, the VrADH can be used as a target spot for regulating the MYMIV by the mung beans and is used for modifying the MYMIV resistance of the mung beans.

The application belongs to the field of biotechnology and medical health, and discloses a casein preparation capable of delaying alcohol absorption and application of the casein preparation in food. The application provides a preparation formula taking casein as a core active ingredient in view of the problems of obvious side effects and insignificant effect of existing alcohol elimination products. The casein preparation can form a colloid condensation layer in gastric juice to protect gastric mucosa, delay the absorption speed of alcohol in the gastrointestinal tract, reduce the damage of alcohol to the gastrointestinal tract and liver, and up-regulate the activity of liver ethanol dehydrogenase ADH and acetaldehyde dehydrogenase ALDH to accelerate alcohol metabolism. The casein preparation can reduce the drunken rate after acute alcohol intake, significantly prolong the drunken time, has excellent alcohol absorption delaying effect and eating safety, and is suitable for preparing various alcohol absorption delaying, liver and stomach protecting food.

The application discloses an activated ethanol dehydrogenase bird's nest peptide and application thereof, and belongs to the technical field of functional polypeptides. The amino acid sequence of the activated ethanol dehydrogenase bird's nest peptide provided by the application is PLLW, can chelate an active center of ethanol dehydrogenase (ADH), helps to prevent oxidation of ADH and further maintain normal protein molecular structure of ADH during alcohol metabolism; the application further provides a bird's nest peptide powder containing the activated ethanol dehydrogenase bird's nest peptide, the bird's nest peptide powder can effectively improve an alcohol catalytic conversion rate of ADH, has a good effect of promoting alcohol metabolism, can be applied to development of various alcoholism-relieving products, and has high market value.