223 results about "Ethanol dehydrogenase" patented technology

The invention relates to a method for biosynthesis of tyrosol in Escherichia coli and application of tyrosol, which belongs to the field of bioengineering technology. According to the method for biosynthesis of tyrosol in Escherichia coli, tyrosine or glucose is used as a substrate, (4-hydroxyphenyl)pyruvic acid is produced under the catalysis of ammonialyase coded by Escherichia coli; (4-hydroxyphenyl)acetaldehyde is produced under the action of the microzyme 4-hydroxyphenylpyruvate decarboxylase; (4-hydroxyphenyl)acetaldehyde is catalyzed by alcohol dehydrogenase so as to produce tyrosol; and a phenylacetaldehyde dehydrogenase gene of Escherichia coli is knocked out at the same time so as to block the transformation channel for (4-hydroxyphenyl)acetaldehyde into (4-hydroxyphenyl)acetic acid and promote accumulation of (4-hydroxyphenyl)acetaldehyde and transformation of (4-hydroxyphenyl)acetaldehyde into tyrosol. The invention provides a novel production approach for tyrosol; and the method lays a foundation for large-scale industrial production of tyrosol and has important economic values and social benefits.

A fusion protein of pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) and a cytochrome is disclosed. PQQGDH is, for example, a water-soluble PQQGDH derived from Acinetobacter calcoaceticus. The cytochrome is, for example, an electron transfer domain of quinohemoprotein ethanol dehydrogenase from Comamonas testosteroni. The fusion protein of the present invention shows intramolecular electron transfer from PQQ, a redox center, to the cytochrome, which allow construction of a direct electron transfer-type glucose sensor which requires no electron mediators.

Bacterial polynucleotides and polypeptides are provided in which the polypeptides have a dehydrogenase activity, such as an alcohol dehydrogenase (ADH) activity, an uronate, a 4-deoxy-L-erythro-5-hexoseulose uronate (DEHU) ((4S,5S)-4,5 dihydroxy-2,6-dioxohexanoate) hydrogenase activity, a 2-keto-3-deoxy-D-gluconate dehydrogenase activity, a D-mannuronate hydrogenase activity, and/or a D-mannnonate dehydrogenase activity. Methods, enzymes, recombinant microorganism, and microbial systems are also provided for converting polysaccharides, such as those derived from biomass, into suitable monosaccharides or oligosaccharides, as well as for converting suitable monosaccharides or oligosaccharides into commodity chemicals, such as biofuels. Commodity chemicals produced by the methods described herein are also provided.

The invention relates to the technical field of clinical detection, in particular to a detection reagent for the content of alcohol in saliva. The invention discloses the detection reagent for the content of the alcohol in the saliva, which is characterized in that 1.25 to 0.5U of alcohol dehydrogenase, 0.05 to 0.2mg of coenzyme NAD and 0.025 to 0.2mg of tetrazolium salt are loaded and contained in a solid phase or semi-solid phase carrier with the area of 5.0*5.0mm<2>. The detection reagent for the content of the alcohol in the saliva has high specificity, high sensitivity, and quick reaction, does not contain poisonous substances, can detect orally, is safe and reliable, shows a result without a composite sensor host, can directly contrast readings with a colorimetric card, has better thermal stability, and can be preserved for 2 years at room temperature. The reagent has the advantages of good repetitiveness of determination results, convenient carrying, simple preparation technology, low cost, easy industry promotion and convenient field detection, and can be used for the detection of drunk drivers and taken as a simple tool for postmortem examination of judicial doctors.

The invention discloses an Escherichia coli engineering strain having high phenethyl alcohol yield and application thereof. An Escherichia coli gene engineering bacterium capable of substantially accumulating phenylalanine (L-Phe) is used as an original strain (the fermentation level of the shake flask fermented phenylalanine can be up to 6.0g/L); phenylpyruvic acid decarboxylase (KDC) and alcohol dehydrogenase (ADH1) are subjected to independent expression and appropriate gene combination to co-express two key enzyme genes so as to construct a recombinant strain; the recombinant strain capable of co-expressing phenylpyruvic acid decarboxylase and alcohol dehydrogenase can produce 130mg/L of beta-phenethyl alcohol, and the phenylalanine yield is 5.0g/L; and the accumulation of phenethyl alcohol can not be detected in the whole fermentation process of the Escherichia coli having high L-Phe yield of the original strain used as a control strain. The invention provides a method for enabling Escherichia coli to directly produce phenethyl alcohol through the fermentation of glucose used as a unique carbon source by means of a metabolic engineering means by using enzyme genes which can realize over-expression and are derived from anabolic pathways of different hosts.

The invention relates to a composition with disintoxicating and liver-protecting effects, which comprises the following raw materials in percentage by mass: 0.15 to 5 percent of crucumin, 0.04 to 1.0 percent of zinc lactate, 10 to 50 percent of glutamine, 10 to 50 percent of alanine and 10 to 50 percent of xylooligosaccharide. It is proved that, by an animal experiment and a human tasting experiment, the composition increases the alcohol dehydrogenase and acetaldehyde dehydrogenase content of a body and rapidly decomposes alcohol, so that the composition has the effects of promoting alcohol metabolism in a liver and reducing the alcohol concentration and active oxygen concentration in a blood; and the composition increases an endurance capacity of a human body to the alcohol, reduces the discomforts such as headache, nausea, vomiting and the like due to the alcohol, and reduces the damages of the alcohol to the liver. The composition can be prepared into food and health-care food with the disintoxicating and liver-protecting effects, can be used as a disintoxicating product for preventing and improving excessive drinking, and can be used as an adjuvant therapy product for an alcoholic liver, a fatty liver due to the alcohol or non-alcohol and protecting the liver; and the composition has the advantages of safety, non-toxicity and a reliable curative effect.

The invention discloses a strain of engineering bacteria producing DL-alanine. Lactic dehydrogenase, pyruvate formate lyase, alcohol dehydrogenase, acetic acid kinase, fumaric acid reductase, alanine racemase and methyl glyoxal synthetase of the strain of engineering bacteria producing the DL-alanine are inactivated; and exogenous L-alanine dehydrogenase gene and alanine racemase gene are integrated on the chromosome of the engineering bacteria. According to the invention, pyroracemic acid, an intermediate product of the glycolysis is converted to L-alanine by integrating the exogenous L-alanine dehydrogenase gene into the chromosome of the engineering bacteria; and an exogenous alanine racemase gene is further integrated into the chromosome, and part of the L-alanine is converted into D-alanine. Then producing the DL-alanine from raw material sugar in one step is realized, the production period of the DL-alanine is decreased and the productivity of the DL-alanine is enhanced.

The invention discloses a food or hygienic food manufacturing method with goat milk or utility in the relieving or neutralizing the effect of alcohol fact, which is composed of goat milk homogenating material and necessary extracted adjunct.

The invention discloses a composite probiotic beverage for dispelling the effects of alcohol and special composite probiotic thereof. The composite probiotic is composed of streptococcus thermophilus,lactobacillus paracasei, lactobacillus acidophilus, lactobacillus bulgaricus, lactobacillus plantarum, bifidobacterium longum and bifidobacterium breve according to a CFU ratio of 2: 3: 4: 1: 1: 1: 3. The composite probiotic beverage for dispelling the effects of alcohol is composed of composite probiotic powder, prebiotics, amino acid and fruit powder. Through use of seven kinds of active probiotics, the composite probiotic beverage can adjust the balance of intestinal flora, improve the immunity of the human body, effectively remove acetaldehyde in the small intestine and colon and relievethe symptoms of headache, dizziness and the like caused by drinking, can be colonized in the intestines, can prevent intestinal flora disturbance caused by drinking, can reduce liver cell damage and can effectively protect liver health. The probiotics contain alcohol dehydrogenase and acetaldehyde dehydrogenase and can effectively decompose alcohol through synergizing with human alcohol dehydrogenation and acetaldehyde dehydrogenase.

The invention relates to a composition for dispelling the effects of alcohol and protecting the liver. The composition comprises the following raw materials in parts by weight: 15-20 parts of extracts from pueraria, 15-20 parts of flower of kudzuvine extracts, 8-12 parts of turnjujube extracts and 45-55 parts of dandelion extracts. Proved by the in-vitro ADH (alcohol dehydrogenase) activity test and the ORAC (oxygen radical absorption capacity) test, the composition provided by the invention has the capability of improving the activity of the ADH timely and effectively, and can accelerate the alcohol metabolism, meanwhile can efficiently remove free radicals, can reduce the oxidative damage caused by alcohol, can prevent undesirable effects of drunkness, dizzy giddy, alcoholic liver injury and the like which are caused by heavy drinking one time and long-time drinking, can be prepared into food and healthcare food which can dispel the effects of alcohol and protect the liver, and can act as auxiliary products and the like for dispelling the effects of alcohol instantly and protecting the liver.

The invention discloses a kit for detecting alcoholic liver disease susceptibility. The kit detects three genes closely related with an alcoholic liver disease, namely an ADH2 gene of alcohol dehydrogenase, an ALDH2 gene of acetaldehyde dehydrogenase and a CYP2E1 gene of cytochrome P4502E1. The simultaneously detected SNP sites comprise an rs1229984 site of the ADH2 gene, an rs671 site of the ALDH2 and an rs2031920 site of the CYP2E1. Whether a detected crowd carries ''alcoholic liver disease susceptible genes'' can be clearly judged by using the kit, detecting a group of genes and sites related with the alcoholic liver disease susceptibility, using specific primers and probes and combining mononucleotide extension technology and micro array chip technology, and the alcoholic liver disease susceptible crowd is screened from the crowd, so that poor life habits are changed and the purpose of prevention is achieved.

The invention relates to preparation of a health-care product for relieving alcohol and preventing getting drunk, which prepares capsules for oral taking by using mixture of high-activity alcohol dehydrogenase and aldehyde dehydrogenase as a main ingredient, adding an enzyme stabilizer such as ferric ions, freeze-drying and adding conventional auxiliary materials. The health-care product has remarkable effects of relieving alcohol and preventing getting drunk. After a drinker takes the product before drinking, alcohol can metabolize quickly in the gastrointestinal tract into acetic acid to beabsorbed or excreted, so the damage of the alcohol to the human body is lowered.

The invention discloses lactobacillus plantarum PLH1405. The lactobacillus plantarum PLH1405 is already collected in The China General Microbiological Culture Collection Center, has a collection number of CGMCC No. 12464, can be used for improving the activity of alcohol dehydrogenase and acetaldehyde dehydrogenase of an organism and accelerating the metabolism of alcohol and acetaldehyde, meanwhile, can be used for increasing the activity of antioxidase and reducing the generation of endotoxin, and can be used for better relieving the damage, which is brought by the alcoholic intoxication, to the organism, and is used as a medicine for treating the acute alcoholic intoxication.

The invention relates to a colon bacillus alcohol fermentation engineering bacterium resisting alcohol. A colon bacillus mutant strain highly resisting the alcohol is obtained through a plurality of generations of directional screening; and an alcohol dehydrogenase gene II and a pyruvate decarboxylase gene of zymomonas mobilis are transferred into the mutant strain to obtain the novel colon bacillus alcohol fermentation engineering bacterium. When the engineering bacterium uses pentose and hexose as substrates to ferment for producing the alcohol, the engineering bacterium can still maintain higher fermentation rate under high-concentration alcohol, and has higher transformation rate of the alcohol.

The invention relates to brachybacterium nesterenkovi new strain and its culturing condition, physiological biochemical characteristic, and enzyme manufacturing method. It includes the following steps: preparing soil solution; gradient diluting; coating the given 200ul solution to 30ul beef extract culture medium; adding 100ul-800ul alcohol inducer ^[12-14]; culturing at 30-40 centigrade degree for 16-48h; purifying for many times; it is single cloning strain by microscopic examination; liquid fermenting; ultrasonic wave smashing; centrifugal separating; purifying; and testing its activity. The advantages of the invention are that the strain can produce acetadehyde dehydrogenase and alcohol dehydrogenase; its culturing condition is simple and low cost to be good for industrialization production; it has high activity at 37 centigrade degree and can impel alcohol to metabolize in human body.

The invention belongs to the field of electrochemical biological fuel cells, and discloses a graphene-based enzyme-modified anode of a biological fuel cell and a preparation and an application. The preparation method comprises the following steps: carrying out a surface pretreatment on a base electrode; putting the pretreated base electrode into a graphene solution, and carrying out an electrochemical polymerization by a cyclic voltammetry scan method to obtain a GN-modified electrode; putting the GN-modified electrode into a water solution containing pyrrole, lithium perchlorate and sodium bicarbonate, carrying out the electrochemical polymerization by a potentiostatic method and modifying a layer of polypyrrole; dropwise adding a composite enzyme solution containing chitosan, ethanol dehydrogenase and acetaldehyde dehydrogenase to the surface of the electrode, and airing the electrode to obtain the modified electrode with an enzyme layer; and soaking and crosslinking the electrode in a glutaraldehyde solution, so as to obtain the graphene-based enzyme-modified anode of the biological fuel cell. The electrode disclosed by the invention is low in cost, good in catalytic performance and wide in applicable substrate range, and has a good application prospect.

The invention relates to a drug for relieving hangover and a preparation method thereof. The drug is prepared from acetic acid bacteria microorganism thallus as raw material by extracting to obtain mixture containing high activity alcohol dehydrogenase and acetaldehyde dehydrogenase, optionally adding adjuvants, and making into oral capsule and granule by conventional method. The alcohol can be rapidly metabolized into acetic acid in gastrointestinal tract to be further absorbed or excreted when the drug is administered by alcohol user before drinking, thereby reducing injury of excessive alcohol to human body to have good hangover relieving effect. The drug can be also made into anti-hangover health food.

The invention provides a preparation method of sea-buckthorn seed polypeptide used for sobering up from drunkenness. The preparation method specifically includes: taking skim sea-buckthorn seed residue as a raw material; adding papain, and hydrolyzing under a most suitable condition; using an ultrafiltration membrane of 3000Da in molecular weight cut-off to separate enzymatic hydrolysate to obtain a sea-buckthorn seed sobering-up polypeptide solution smaller than 3000Da in molecular weight. In-vitro experiments show that the polypeptide solution has obvious activating effect on alcohol dehydrogenase, and activity of polypeptide from 500Da to 2000Da is highest; the polypeptide can promote decomposition and metabolism of in-vivo alcohol to realize sobering up from drunkenness by enhancing activity of alcohol dehydrogenase. Animal experiments show that the polypeptide can play a role in preventing drunkenness and sobering up from drunkenness if being taken in by 200mg/kg.bw and can be used as a raw material of sobering-up and healthcare products.

The invention discloses an amygdalus persica/amygdalus davidiana resin polysaccharide embedded natural antioxidant composition and an application thereof. The composition can effectively prevent intoxication caused by single-time heavy-dosage wine drinking and relevant organism health risks after intoxication. The composition is prepared through the following steps of in an aqueous solution system, using amygdalus persica/amygdalus davidiana resin polysaccharide as a wall material, and performing micro nanocrystallization embedding on a natural antioxidant so as to obtain the composition. Preferably, in the composition, the mass concentration of the amygdalus persica/amygdalus davidiana resin polysaccharide is not greater than 5%g/mL, and the optimal mass concentration is 1%-2.5%g/mL. According to the amygdalus persica/amygdalus davidiana resin polysaccharide embedded natural antioxidant composition disclosed by the embodiment of the invention, the amygdalus persica/amygdalus davidianaresin polysaccharide forms a protective barrier resistant to digestive enzyme decomposition in gastrointestinal tracts, so that chemical damage of high-concentration ethanol to stomach and intestinemucosa can be avoided or reduced; and natural antioxidant molecules through micro nanocrystallization embedding of the amygdalus persica/amygdalus davidiana resin polysaccharide can restrain oxidativestress cascading damage caused by ethanol metabolism, the activity of alcohol dehydrogenase and acetaldehyde dehydrogenase in bodies can be activated and increased, and ethanol metabolism can be promoted.