136 results about "Ethanol dehydrogenase" patented technology

The invention relates to Pediococcus pentosaceus PP90 for preventing and treating alcoholic nerve injury and application of the Pediococcus pentosaceus PP90, the Pediococcus pentosaceus PP90 is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.32034. The Pediococcus pentosaceus PP90 can be used for preventing and treating alcoholic nerve injury. The fermentation product of the pediococcus pentosaceus PP90 can degrade the alcoholic strength of alcohol, can generate ethanol dehydrogenase and acetaldehyde dehydrogenase, can improve the activity of nerve cells, can regulate the expression of cytokines, can inhibit the activation of astrocytes, can regulate the expression of brain-derived neurotrophic factors, and can inhibit the growth of the brain-derived neurotrophic factors. The compound can improve the disordered neurotransmitter level, and can be used for preparing products for preventing or improving alcoholic nerve injury.

The invention provides a recombinant pichia kudriavzevii strain and application thereof, pichia kudriavzevii is taken as an original strain, and a reduced TCA pathway is introduced and comprises the following steps: overexpressing fumarate reductase FRD in the strain; weakening or inactivating an ethanol pathway, comprising: knocking out any one or a combination of at least two of encoding genes of ethanol dehydrogenase ADH1, ADH3 or ADH6; enhancing the PPP pathway in the pentose phosphate pathway of the strain, including: overexpressing glucose-6-phosphate dehydrogenase ZWF1 and / or 6-phosphogluconate dehydrogenase GND1; the method for reducing the activity of the malate dehydrogenase MDH2 in the TCA circulation pathway comprises the step of knocking out the coding gene of the malate dehydrogenase MDH2 in the TCA pathway. The pichia kudriavzevii is modified by using genetic engineering and metabolic engineering methods, so that the yield and the yield of succinic acid are increased, and the pichia kudriavzevii has important application prospects in industrial production.

The invention provides application of a lactobacillus mucus fermentation strain A21196 in preparation of a product for promoting ethanol metabolism, and belongs to the technical field of microbial preparations. The lactobacillus mucus fermentation strain A21196 has the high-yield ethanol dehydrogenase and acetaldehyde dehydrogenase, and has the effects of reducing the content of ethanol and acetaldehyde in the blood of mice suffering from acute alcoholism and shortening the central nervous inhibition time in vivo. Therefore, the invention provides the application of the fermented lactobacillus mucus strain A21196 in preparation of a product for preventing and / or treating the alcoholic liver injury or the alcoholic liver disease.

The invention provides a construction method and application of recombinant yarrowia lipolytica with high yield of tyrosol, and belongs to the field of bioengineering. The recombinant yarrowia lipolytica with high yield of tyrosol is named as a yarrowia lipolytica XJ-TL strain, and the recombinant yarrowia lipolytica with high yield of tyrosol is named as a yarrowia lipolytica XJ-TL strain. The recombinant yarrowia lipolytica disclosed by the invention expresses an ethanol dehydrogenase encoding gene, and simultaneously expresses any one or a combination of more of a DAHP synthase mutant encoding gene, a chorismate mutase mutant encoding gene, a glucose 6-phosphate dehydrogenase encoding gene, a transketolase encoding gene and a transaldolase encoding gene; meanwhile, a natural promoter of a pyruvate kinase coding gene is truncated. After the recombinant yarrowia lipolytica XJ-TL strain is subjected to fed-batch fermentation, tyrosol can be synthesized at a relatively high level. The construction method of the recombinant yarrowia lipolytica is easy to operate, and efficient synthesis of tyrosol can be achieved.

The invention provides application of acidophilous glycosyltransferase in salidroside production, and belongs to the technical field of biological engineering. The problem of producing salidroside under the acidic condition is solved. Comprising an application of acidophilous glycosyl transferase with an amino acid sequence as shown in SEQ ID NO.1 in salidroside production under an acidic condition and an acidophilous escherichia coli engineering strain for producing salidroside. The escherichia coli engineering strain overexpresses a mutant 3-deoxy-D-arabinoheptulose-7-phosphate (DAHP) synthetase gene aroGfbr, a cyclohexadiene dehydrogenase gene tyrC, a glucose phosphate mutant enzyme gene pgm and a UDP-glucose pyrophosphorylase galU, overexpresses a phenylpyruvate decarboxylase gene ARO10 derived from saccharomyces cerevisiae, and can be used for producing a mutant 3-deoxy-D-arabinoheptulose-7-phosphate mutant enzyme. The kit comprises an ethanol dehydrogenase gene ADH6 and a glycosyl transferase gene LrUGT85AF8. The method is mainly used for producing salidroside under an acidic condition.

The invention relates to pediococcus acidilactici PA27 capable of degrading alcohol and improving alcoholic nerve injury and application of the pediococcus acidilactici PA27, the pediococcus acidilactici PA27 is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.31922. The pediococcus acidilactici PA27 can degrade alcohol and improve alcoholic nerve injury. The pediococcus acidilactici PA27 separated and screened by the invention can grow and propagate in an alcohol-containing culture medium, can degrade alcohol and can generate ethanol dehydrogenase and acetaldehyde dehydrogenase; the polypeptide can be used for constructing a blood-brain barrier and reducing the damage of alcohol to central nervous cells; the traditional Chinese medicine composition can reduce the expression of inflammatory cytokines TNF-alpha, inhibit the activation of astrocytes, promote the expression of brain-derived neurotrophic factors BDNF, regulate the release of alcohol-induced astrocyte neurotransmitters and improve alcoholic nerve injury.

The invention relates to the field of medicines, and particularly discloses application of mogroside V in preparation of a medicine for dispelling the effects of alcohol and protecting the liver, experiments prove that the anti-alcohol effect of the mogroside V is obviously superior to that of a mogroside crude extract, and in one embodiment, the mogroside V can increase protein expression of ethanol dehydrogenase and acetaldehyde dehydrogenase in the liver of a mouse at the same time. In one embodiment, the mogroside V can prolong the drunkenness tolerance time of a mouse and shorten the drunkenness time of the mouse. In another embodiment, the mogroside V can relieve liver inflammation caused by alcohol. In a further embodiment, the mogroside V can greatly relieve liver fat deposition caused by alcohol. Therefore, the mogroside V can be developed into a brand new product for dispelling the effects of alcohol and protecting the liver, and has a huge application prospect.

The invention discloses ethanol dehydrogenase, recombinant genetically engineered bacteria and application of the ethanol dehydrogenase in asymmetric reduction of prochiral carbonyl compounds, the ethanol dehydrogenase has good biological performance, heterologous expression in Escherichia coli engineering bacteria is achieved, intracellular coenzyme can be directly utilized when whole cells are adopted as a catalyst, and the catalyst has a good application prospect. Zero addition of the coenzyme is realized, the enzyme has the advantages of high activity, high stereoselectivity, wider substrate spectrum and the like, prochiral ketone compounds can be greenly and efficiently catalyzed to be converted into corresponding chiral alcohols, and the problems of low conversion rate, low yield, low e.e. Value, complex reaction process, more byproducts and the like in a traditional chemical method are solved. The ethanol dehydrogenase has good tolerance to isopropanol, and the isopropanol can be used as a hydrogen source to realize the circulation of coenzyme. And the byproduct acetone generated by the enzyme catalytic reaction and the isopropanol remaining in the reaction can be separated and recovered by a technical means, so that the production cost is greatly reduced.

The present invention relates to the field of microbial fermentation technology, and in particular to a Pediococcus pentosaceus ZKPp0412, Pueraria lobata fermentation liquid and their application in preparing alcohol sobering and liver protecting products. Pediococcus pentosaceus )ZKPp0412 was deposited in the General Microbiology Center of the China Microbiological Culture Collection Administration on June 21, 2023, with the deposit number CGMCC No.27674, and the deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing. The Pueraria lobata fermentation liquid prepared by fermenting the Pueraria lobata extract by this strain has a lower pH and a higher number of viable bacteria, and can promote the activity of alcohol dehydrogenase and acetaldehyde dehydrogenase in vitro. In vivo experiments have verified that the Pueraria lobata fermentation liquid has the effect of sobering up and sobering up drunk mice, and can effectively inhibit alcohol-induced liver enlargement / liver damage.

The invention relates to a medicinal and edible plant composition and application thereof. The medicinal and edible plant composition is an aqueous extract of the following preparation raw materials: kudzuvine root, hovenia dulcis thunb, chrysanthemum bud, poria cocos and liquorice. According to the invention, five specific medicinal and edible raw materials are reasonably combined to prepare a water extraction product, the product has an excellent anti-oxidation effect, has an activation effect on ethanol dehydrogenase and acetaldehyde dehydrogenase, and has very good potential of dispelling the effects of alcohol and protecting the liver, and all the components are mutually matched and have a synergistic effect on the effects.

The invention discloses a genetically engineered probiotic composition for expressing ADH (adenosine dihydrogenase) and ALDH (aldehyde dehydrogenase) and application of the genetically engineered probiotic composition in hangover alleviating and liver protection. The genetically engineered probiotic composition (such as EcN.1917-ADH and EcN.1917-ALDH) is constructed by introducing genes of alcohol dehydrogenase (ADH, NCBI (National Center for Biotechnology Information) Gene ID: 855349) and acetaldehyde dehydrogenase (ALDH, NCBI Gene ID: 855206) of saccharomyces cerevisiae into probiotics (such as escherichia coli). The engineering bacterium shows remarkable ethanol, acetaldehyde and endurance capacity in an in-vitro experiment, and the ethanol degradation rate is remarkably higher than that of a wild type strain; a mouse drunkenness model verifies that drunkenness and death caused by drinking can be reduced through pretreatment, drunkenness tolerance time is remarkably prolonged, and movement coordination disorder caused by alcohol can be improved. The strain can effectively promote alcohol metabolism and relieve alcoholic liver injury, and a new candidate strain is provided for development of a hangover-alleviating and liver-protecting viable bacterial preparation.

The invention discloses lactobacillus plantarum WL05 capable of efficiently degrading ethanol and application of the lactobacillus plantarum WL05, and belongs to the technical field of biology. The bacterial strain is lactobacillus plantarum WL05, the bacterial strain is preserved in the China General Microbiological Culture Collection Center on July 8, 2024, and the preservation number is CGMCC No.31202. The lactobacillus plantarum WL05 has relatively high acid production capacity and activity, and the lactobacillus plantarum WL05 is preserved in the China General Microbiological Culture Collection Center on July 8, 2024. The acid resistance and cholate resistance are high; after the lactobacillus plantarum WL05 is fermented in an MRS culture medium containing 10% ethanol, the ethanol degradation rate can reach 42.5%, and the ethanol dehydrogenase activation rate is 43.53%; the fermentation liquor is inoculated into a lalang grass rhizome-reed rhizome matrix culture medium, and after fermentation, the measured ethanol degradation rate is 63.9%, and the activation rate of ethanol dehydrogenase in the fermentation liquor is 66.5%. The strain has strong reducing ability and antibacterial ability at the same time. The ethanol degradation rate of the beverage prepared based on the strain can reach 32%. The product can be used for preparing functional food for dispelling effects of alcohol and protecting liver, and has high application value in the field of functional food.

The invention belongs to the technical field of bioengineering, and particularly relates to kluyveromyces marxianus engineering bacteria for synthesizing eicosapentaenoic acid and application of the kluyveromyces marxianus engineering bacteria. According to the engineering bacterium, pyruvate decarboxylase PDC1 genes, alcohol dehydrogenase ADH1 genes and alcohol dehydrogenase ADH2 genes are sequentially knocked out from a kluyveromyces marxianus FIM1 delta ura3 strain; meanwhile, a citrate lyase ACL gene, a pyruvate oxidase PO gene and a phosphate transacetylase PTA gene are inserted into the loci respectively, so that synthesis of acetyl coenzyme A is enhanced; then a plurality of genes are sequentially inserted into a Kluyveromyces marxianus genome, and then a vector of a delta 8 desaturase delta 8D gene is transferred into the Kluyveromyces marxianus genome. The invention also provides a method for efficiently preparing the EPA, namely the Kluyveromyces marxianus engineering strain is used as a fermentation strain, glucose, a yeast extract, peptone and other components are used as a culture medium, and the yield of the EPA produced by culturing and fermenting reaches 8.71 mg / g of dry cell weight.

The present invention relates to the field of microbial technology, and provides a fermented mucus lactobacillus YYS-K2 and an application thereof. The deposit number of the fermented mucus lactobacillus YYS-K2 is CGMCC No.27129. The fermented mucus lactobacillus YYS-K2 can produce ethanol dehydrogenase (ADH), has good ethanol tolerance and degradation ability, can grow in a high-concentration ethanol culture medium, and at the same time, the bacteria has a significant inhibitory effect on α-amylase inhibition, has significant OH and DPPH free radical scavenging ability, and has good reducing ability. The bacteria can provide a new probiotic source for the development of functional products such as hangover products, antioxidant products and weight loss products (α-amylase inhibition functional products), and has important application value.

The invention relates to the technical field of traditional Chinese medicines, in particular to a hangover-alleviating, liver-protecting and stomach-protecting agent and a preparation method thereof. The alcohol effect dispelling, liver protecting and stomach protecting agent is prepared from the following raw material components in parts by mass: 1-10 parts of peristrophe japonica, 1-10 parts of penthorum chinense pursh, 1-10 parts of flos puerariae, 1-10 parts of radix puerariae, 1-10 parts of lalang grass rhizome, 1-10 parts of hovenia dulcis thunb and 1-5 parts of taurine. The traditional Chinese medicine composition has better hangover-alleviating and liver-protecting effects, obviously shortens the hangover-alleviating and sobering time, is taken before drinking, takes effect faster, has the effects of alleviating hangover, inducing resuscitation, strengthening the spleen and stomach, calming the adverse-rising energy, arresting vomiting and protecting the liver, reduces AST and ALT in human serum, can enhance the activity of ethanol dehydrogenase and acetaldehyde dehydrogenase in the human liver, and promotes ethanol metabolism.

The invention discloses a plant lactobacillus and its application. The plant lactobacillus is isolated from the intestinal tract of a healthy human body and can 7 CFU / mL), has strong alcohol sobering ability, and tolerates a certain concentration of bile salt and acid environment in the human intestine; the strain has significant synergistic degradation ability of ethanol and acetaldehyde, can quickly decompose ethanol, shorten the drunkenness time of KM male mice, which is shortened by more than 52% compared with the model group, and the drunkenness tolerance time is extended by more than 212%. At the same time, it enhances the activity of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) in the mouse liver, effectively reduces the acetaldehyde content in the mouse blood, can be used to prepare bacterial agents for preventing and / or relieving drunkenness, and provides a new candidate strain for the preparation of live bacteria preparations for sobering up.

The invention discloses a construction method of an escherichia coli mutant for producing succinic acid by fermentation of a synthetic medium. The method comprises the following steps: firstly, knocking out a lactic dehydrogenase gene ldhA, a pyruvate formate lyase gene pflB, a ptsG gene responsible for encoding a phosphotransferase system EIIBC protein, an ethanol dehydrogenase gene adhE, an acetokinase-phosphate transacetylase gene ackA-pta, and a ptsG gene responsible for encoding a phosphotransferase system EIIBC protein in escherichia coli; a phosphoenolpyruvate carboxykinase gene pck from bacillus subtilis is integrated at an SS9 safety site of a strain to obtain escherichia coli ESC6 with high succinic acid yield; and mutating one or more loci in one or more genes of a glucose-transcriptional inhibition factor gene mlc, a nitrate response regulatory factor gene narL and a cyclic adenylate receptor protein gene crp to obtain the escherichia coli mutant capable of producing succinic acid by fermentation of a synthetic culture medium, wherein the one or more loci in one or more genes of the glucose-transcriptional inhibition factor gene mlc, the nitrate response regulatory factor gene narL and the cyclic adenylate receptor protein gene crp are mutated. The strain can be fermented in a synthetic medium to produce succinic acid, so that the fermentation cost is greatly reduced, and the strain has a very wide application prospect.

The invention discloses an ethanol dehydrogenase mutant and an application of the ethanol dehydrogenase mutant in synthesis of symmetric chiral diol. The ethanol dehydrogenase mutant is obtained by carrying out single mutation or multiple mutations on amino acids at 74th, 187th, 210th, 211th and 352th sites of an ethanol dehydrogenase amino acid sequence as shown in SEQ ID NO.2 from Candida orbiculata Co. The invention further discloses a preparation method of the ethanol dehydrogenase mutant, and the preparation method comprises the following steps: carrying out single mutation or multiple mutations on the amino acids at the 74th, 187th, 210th, 211th and 352th sites of the ethanol dehydrogenase amino acid sequence as shown in SEQ ID NO.2. Alcohol dehydrogenase is utilized to catalyze prochiral symmetric diketone to synthesize symmetric chiral diol, the method is high in substrate concentration, high in stereoselectivity, mild in reaction, simple in operation process, environment-friendly and more suitable for industrial amplification production, and the problems that in the prior art, the substrate concentration is not high, the reaction condition is violent, the reaction process is complex and difficult to control, and the production cost is low are solved. And the method is not environment-friendly enough, and the like.

The invention relates to the technical field of genetic engineering, in particular to a recombinant microorganism and application thereof in preparation of glycine. The application comprises the following steps: producing glycine by adopting the recombinant microorganism; the recombinant microorganism comprises an enzyme combination and / or a nucleic acid molecule combination. The enzyme combination comprises threonine aldolase, ethanol dehydrogenase and isopropanol dehydrogenase; the threonine aldolase comprises an amino acid sequence as shown in SEQ ID NO. 1. The nucleic acid molecule combination is used for encoding the enzyme combination. According to the invention, threonine aldolase, ethanol dehydrogenase and isopropanol dehydrogenase are converted into microorganisms, and the constructed recombinant microorganisms can be used for decomposing threonine to produce glycine; and a specific system formed by specific isopropanol dehydrogenase, ethanol dehydrogenase and isopropanol dehydrogenase can also have a relatively high conversion rate under the condition of no addition of pyridoxal phosphate (PLP), and has important significance and economic value.

The invention belongs to the technical field of microorganisms, and particularly relates to lactobacillus paracasei BL19 and application thereof to preparation of products with functions of dispelling effects of alcohol and protecting liver. The lactobacillus paracasei BL19 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the lactobacillus paracasei BL19 is CGMCC No.34368. The lactobacillus paracasei BL19 is named as Lactobacillus paracasei BL19. The lactobacillus paracasei BL19 provided by the invention has relatively strong alcohol tolerance, alcohol degradation capability and relatively high activity of promoting alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH); the lactobacillus paracasei BL19 has the advantages that the lactobacillus paracasei BL19 has good anti-alcohol and anti-liver effects and strong capacity of protecting alcoholic injury liver cells, so that the anti-alcohol and anti-liver effects can be comprehensively achieved from multiple dimensions, and in addition, the lactobacillus paracasei BL19 has strong acid resistance and bile salt resistance, also has strong tolerance to simulated gastrointestinal fluid and has important application value in the fields of anti-alcohol and anti-liver products and the like.

The present invention discloses a method for synthesizing hydroxytyrosol by multi-enzyme cascade, comprising: 1) preparing a hydroxytyrosol product by using 3,4-dihydroxybenzaldehyde, L-threonine, sodium formate, NAD + As raw material, L-threonine transaldolase, alcohol dehydrogenase, and formate dehydrogenase are used to catalyze the synthesis of L-threo-3-(3,4-dihydroxyphenyl)serine; 2) L-threo-3-(3,4-dihydroxyphenyl)serine is used as substrate, glucose, NAD + Hydroxytyrosol is synthesized through a cascade catalysis of phenylserine dehydratase, α-ketoacid decarboxylase, aldehyde reductase, and glucose dehydrogenase. The synthesis method provided by the present invention achieves a hydroxytyrosol yield of >99% and a space-time yield of 0.88 g / L / h, currently the highest, showing great potential for industrial application and suitable for further promotion and application.

The invention relates to the technical field of health food, in particular to a selenium tea ethanol dehydrogenase activating peptide composition as well as a preparation method and application thereof. A response surface method is adopted to optimize a TSE-PP preparation process, affinity ultrafiltration and LC-MS / MS technologies are utilized to screen ADH binding peptides, and the action mechanism of the ADH binding peptides is researched through molecular docking and animal experiments. Experiments prove that the TSE-PP can promote ethanol metabolism, relieve liver cell injury, inhibit inflammatory response, relieve oxidative stress and improve liver lipid accumulation, the high-dose use effect is more remarkable, and a scientific basis is provided for application of the TSE-PP in the food with the function of dispelling the effects of alcohol and the nutrition fortified product. The invention discloses the preparation process of the TSE-PP and the anti-alcohol and liver protection effects of the TSE-PP, meanwhile, the TSE-PP has good food processing characteristics, and a theoretical basis and an experimental support are provided for production and application of the TSE-PP in functional foods.

The invention relates to the technical field of gene engineering, in particular to an ethanol response type gene engineering alcohol-dissolving probiotic as well as a construction method and application thereof. According to the probiotics provided by the invention, an ethanol-responsive regulation mechanism is innovatively introduced, so that the strain can specifically induce high-efficiency expression of human ethanol dehydrogenase 1B (hADH1B) and human acetaldehyde dehydrogenase 2 (hALDH2) when sensing the existence of ethanol in vivo, thereby realizing on-demand and high-efficiency decomposition of ethanol and toxic metabolite acetaldehyde thereof. Compared with continuous expression of traditional engineering bacteria, the intelligent regulation and control mode can better simulate the natural metabolic rhythm of a human body, solves the problem of bacterial metabolism, reduces the metabolic pressure of thalli while improving the hangover alleviating efficiency, and is expected to achieve a better hangover alleviating effect and higher biological safety.

A transformant obtained by introducing a DNA of (a1), (a2), or (a3) below, and (b) an alcohol dehydrogenase gene, into a bacterium of the genus Hydrogenophilus, can efficiently produce isobutanol utilizing carbon dioxide as a sole carbon source.(a1) DNA which consists of a base sequence of SEQ ID NO: 1;(a2) DNA which consists of a base sequence having 90% or more identity with SEQ ID NO: 1, the DNA encoding a polypeptide having 2-keto-acid decarboxylase activity;(a3) DNA which hybridizes with a DNA consisting of a base sequence complementary to SEQ ID NO: 1 under stringent conditions, and which encodes a polypeptide having 2-keto-acid decarboxylase activity.

The invention relates to the technical field of furfuryl alcohol preparation, in particular to a method for preparing furfuryl alcohol through furfural hydrogenation. According to the method for preparing furfuryl alcohol through furfural hydrogenation, in a hydrogen atmosphere, under the catalytic action of a catalyst and in a reducing atmosphere, a reaction system is formed by taking furfural as a substrate, adding oxidized coenzyme NAD < + > and alcohol dehydrogenase ADH and taking a buffer solution as a reaction medium, and furfuryl alcohol is generated through furfural hydrogenation reaction. According to the method, hydrogenation reduction of furfural is catalyzed through a catalyst coupling cofactor regeneration (NADH) and an enzyme (alcohol dehydrogenase, ADH) reaction to prepare furfuryl alcohol, a high catalytic effect can be obtained under a very mild condition, and the method has a good market prospect.

The invention relates to a new application of early-stage chick embryo amniotic fluid, which is characterized in that the early-stage chick embryo amniotic fluid is found to be capable of effectively dispelling the effects of alcohol, removing the content of ethanol in serum and increasing the levels of liver acetaldehyde dehydrogenase and ethanol dehydrogenase by evaluating the effect of dispelling the effects of alcohol of the early-stage chick embryo amniotic fluid, and also has the effects of protecting the gastric mucosa function and the like.

This invention relates to the field of functional food technology, and more particularly to the application of safflower honey, or safflower honey extract, in enhancing the activity of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH). The invention has found that safflower honey, or safflower honey extract, can effectively enhance ADH and ALDH activity, thereby promoting alcohol metabolism and achieving a hangover-relieving effect. Further research on the extract revealed that a mixture of syringaldehyde, riboflavin, lutein, luciferin, and three p-coumaroyl spermidine plays a key role in enhancing ADH and ALDH activity, thus providing an application of this mixture in hangover relief. The application of safflower honey and its extract provided by this invention has high application value for developing foods and health products with hangover-relieving effects.

The present application provides a kind of kudzu vine cranberry composition for protecting liver from alcohol, which contains the following ingredients by weight: 30-50 parts of extract of Hovenia dulcis Thunb, 30-50 parts of mixed extract, 20-40 parts of extract of Pueraria lobata, 10-20 parts of extract of Poria cocos, 10-20 parts of extract of Glycyrrhiza uralensis, 0.05-0.1 parts of vitamin C; the raw materials of the mixed extract are mixed by cranberry and Fructus Lycii in a mass ratio of (1-2):(1-2). The present application further provides formulations and food containing the composition. The present application further protects the use of the composition or formulation in preparing health food or medicine with auxiliary protective effect on chemical liver injury. The composition provided by the present application can improve the activity of alcohol dehydrogenase / acetaldehyde dehydrogenase, reduce the content of MDA in tissues and increase the content of GSH, and reduce the content of triglyceride in liver cells, thereby comprehensively exerting the effect of protecting liver from alcohol, with high safety, small side effects, and wide sources of raw materials, and having important market value and development prospect.

The application discloses a kind of biological immobilized enzyme based on yeast surface display technology and the application of synthesis 2-phenylethanol, using yeast surface display technology, for the first time with Saccharomyces cerevisiae surface protein a-lectin as anchor protein, the key enzyme (transaminase, decarboxylase, ethanol dehydrogenase) involved in 2-PE producing Ehrlich pathway in Saccharomyces cerevisiae is separately or jointly carried out biological fixation, enhance substrate channel effect and proximity effect, in vitro one-step catalysis L-phenylalanine synthesis 2-PE, provide yield.The method for fixing multiple enzymes of Ehrlich pathway in the application can be used to reconfigure the biosynthesis pathway of other natural products in vitro, to provide feasible scheme for the rational design of cell factory.

The invention relates to the technical field of biology, and discloses a genetically engineered bacterium for high yield of 1, 4-butanediol and application of the genetically engineered bacterium. Escherichia coli is used as a chassis bacterium, a gene ldhA for coding lactic dehydrogenase, a gene adhE for coding ethanol dehydrogenase, a gene pflB for coding formate lyase, and a gene gabD and a gene yneI for coding succinate semialdehyde dehydrogenase are knocked out from a chassis bacterium genome, expression of a succinate semialdehyde dehydrogenase gene sucD and a 4-hydroxybutyrate dehydrogenase gene 4hbD is strengthened, a pdhR gene is knocked out, and a recombinant plasmid is obtained. The expression of the gene gltAR164L is enhanced, the gene mdh, the gene arcA, the gene poxB, the gene sdhAB and the gene ptsG are knocked out, and the expression of the glf gene and the ppc gene is enhanced; the method comprises the following steps of: transferring plasmids carrying a gene ald for coding aldehyde dehydrogenase, a gene cat2 for coding acyltransferase and a gene yqhD for coding alcohol dehydrogenase into a chassis bacterium, and carrying out metabolic transformation to obtain a genetically engineered bacterium which carries exogenous plasmids and has the capabilities of high yield and extracellular accumulation of 1, 4-BDO, so that the yield of 1, 4-BDO can be increased to 4.71 g / L.