Graphene-based enzyme-modified anode of biological fuel cell and preparation and application

A biofuel cell and graphene technology, applied in battery electrodes, nanotechnology for materials and surface science, circuits, etc., can solve problems such as high cost, narrow range of applicable substrates, and complicated preparation methods, and achieve low cost , easy preparation, good catalytic performance

Inactive Publication Date: 2016-01-20
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the enzyme-modified electrodes in the prior art generally have the defects of complicated preparation methods, high cost or narrow range of applicable substrates.

Method used

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  • Graphene-based enzyme-modified anode of biological fuel cell and preparation and application
  • Graphene-based enzyme-modified anode of biological fuel cell and preparation and application
  • Graphene-based enzyme-modified anode of biological fuel cell and preparation and application

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Effect test

Embodiment 1

[0040] (1) The glassy carbon electrode with a diameter of 3mm is used successively with Al with a diameter of 0.3μm and 0.05μm 2 O 3 The powder is polished to a mirror surface, rinsed with distilled water, then ultrasonically cleaned in absolute ethanol and distilled water for 1 min, and then the glassy carbon electrode is placed in 0.5mol / L H 2 SO 4 Activate the electrode in the solution, take it out, rinse with distilled water, and dry to obtain the pretreated glassy carbon electrode;

[0041] (2) Weigh 100 mg of graphene and place it in a beaker, add 50 mL of distilled water, and ultrasound for 24 hours to obtain a 2 mg / mL graphene solution. Place the pretreated glassy carbon electrode in the graphene solution and use cyclic voltammetry Carry out electrochemical polymerization, with a polymerization potential of 0.2~0.9v, scan 18 circles at a speed of 0.05v / s, polymerize a layer of graphene, take it out, rinse, and dry naturally to obtain a GN modified electrode;

[0042] (3) The...

Embodiment 2

[0048] (1) The glassy carbon electrode with a diameter of 3mm is used successively with Al with a diameter of 0.3μm and 0.05μm 2 O 3 The powder is polished into a mirror surface, rinsed with distilled water, then ultrasonically cleaned in absolute ethanol and distilled water for 1 min, and then the glassy carbon electrode is placed in 0.5mol / L H 2 SO 4 Activate the electrode in the solution, take it out, rinse with distilled water, and dry to obtain the pretreated glassy carbon electrode;

[0049] (2) Weigh 100 mg of graphene and place it in a beaker, add 50 mL of distilled water, and ultrasound for 24 hours to obtain a 2 mg / mL graphene solution. Place the pretreated glassy carbon electrode in the graphene solution and use cyclic voltammetry Carry out electrochemical polymerization, with a polymerization potential of 0.2~0.9v, scan 18 circles at a speed of 0.05v / s, polymerize a layer of graphene, take it out, rinse it, and dry it naturally to obtain a GN modified electrode;

[0050]...

Embodiment 3

[0055] (1) The glassy carbon electrode with a diameter of 3mm is used successively with Al with a diameter of 0.3μm and 0.05μm 2 O 3 The powder is polished into a mirror surface, rinsed with distilled water, then ultrasonically cleaned in absolute ethanol and distilled water for 1 min, and then the glassy carbon electrode is placed in 0.5mol / L H 2 SO 4 Activate the electrode in the solution, take it out, rinse with distilled water, and dry to obtain the pretreated glassy carbon electrode;

[0056] (2) Weigh 100 mg of graphene and place it in a beaker, add 50 mL of distilled water, and ultrasound for 24 hours to obtain a 2 mg / mL graphene solution. Place the pretreated glassy carbon electrode in the graphene solution and use cyclic voltammetry Carry out electrochemical polymerization, with a polymerization potential of 0.2~0.9v, scan 18 circles at a speed of 0.05v / s, polymerize a layer of graphene, take it out, rinse it, and dry it naturally to obtain a GN modified electrode;

[0057]...

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Abstract

The invention belongs to the field of electrochemical biological fuel cells, and discloses a graphene-based enzyme-modified anode of a biological fuel cell and a preparation and an application. The preparation method comprises the following steps: carrying out a surface pretreatment on a base electrode; putting the pretreated base electrode into a graphene solution, and carrying out an electrochemical polymerization by a cyclic voltammetry scan method to obtain a GN-modified electrode; putting the GN-modified electrode into a water solution containing pyrrole, lithium perchlorate and sodium bicarbonate, carrying out the electrochemical polymerization by a potentiostatic method and modifying a layer of polypyrrole; dropwise adding a composite enzyme solution containing chitosan, ethanol dehydrogenase and acetaldehyde dehydrogenase to the surface of the electrode, and airing the electrode to obtain the modified electrode with an enzyme layer; and soaking and crosslinking the electrode in a glutaraldehyde solution, so as to obtain the graphene-based enzyme-modified anode of the biological fuel cell. The electrode disclosed by the invention is low in cost, good in catalytic performance and wide in applicable substrate range, and has a good application prospect.

Description

Technical field [0001] The invention belongs to the field of electrochemical biofuel cells, and specifically relates to a graphene-based biofuel cell enzyme modified anode, and preparation and application. Background technique [0002] Enzyme fuel cell is a kind of biofuel cell that directly uses enzymes. Enzyme fuel cells need to work continuously and stably for several months or even longer. This not only requires the electrode modification materials to have a certain degree of biocompatibility, but also requires enzymes to adapt to the human physiological environment or other use environments to maintain long-term working conditions. The catalytic activity of the enzyme. This is still more difficult for oxidoreductases, so the current continuous working time of enzyme fuel cells is relatively short, usually about a few days to a month. Battery life is mainly related to the characteristics of enzymes, and environmental factors such as temperature, pH, and the composition and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H01M4/86H01M4/90H01M4/88B82Y30/00B82Y40/00
CPCB82Y30/00B82Y40/00H01M4/8657H01M4/8673H01M4/8853H01M4/9075H01M2004/8689Y02E60/50
Inventor 朴金花董长城姜建国薛倩然马维宇陈列增
Owner SOUTH CHINA UNIV OF TECH
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