985 results about "Dehydrogenase" patented technology

The invention relates to a plant lactobacillus JN9, a preparation, a probiotic composition and application thereof. According to the application, a new plant lactobacillus JN9 strain is obtained through screening, and the preservation number of the new plant lactobacillus JN9 strain is CCTCC (China Center for Type Culture Collection) NO: M 20241647. Through basic characteristic analysis (growth curve, acid resistance, cholate resistance and gastrointestinal fluid resistance), safety evaluation (antibiotic sensitivity, hemolysis capability, toxin production capability, cytotoxicity and biogenic amine production capability), adhesiveness evaluation, GABA production evaluation and alcohol tolerance and acetaldehyde dehydrogenase production capability evaluation of the strain, the strain is found to be a safe strain; and the GABA has excellent GABA production performance, high alcohol tolerance and high acetaldehyde dehydrogenase activity. The composition can be applied to products for improving mood, such as relieving pressure, resisting depression, improving body sleep quality, reducing blood pressure, resisting diabetes, resisting cancer, resisting oxidation, resisting inflammation, resisting microorganisms, resisting allergy, enhancing alcohol tolerance in drinking, relieving discomfort after drinking and the like.

The present invention relates to a recombinant yeast with high squalene production, a construction method and a use thereof, wherein the recombinant yeast is a saccharomyces cerevisiae that expresses or overexpresses HMGR gene, ADA gene, LEU2 gene, MVA pathway gene and squalene synthase gene ERG9. The present invention integrates acetaldehyde dehydrogenase gene ADA and HMG-CoA reductase gene tHMGR in multiple copies in saccharomyces cerevisiae, integrates the screened high-activity phosphoketolase gene PK and phosphotransacetylase gene PTA, weakens epoxysqualene synthesis, strengthens the mevalonate pathway, overexpresses yeast endogenous squalene synthase gene, etc. to obtain a recombinant yeast with high squalene production, so that the production in the shake flask stage of the production process exceeds 5g / L, and the production in the fed batch fermentation stage exceeds 50g / L, breaking through the current bottleneck of microbial synthesis of squalene production, and is suitable for practical promotion and application.

The invention relates to Pediococcus pentosaceus PP90 for preventing and treating alcoholic nerve injury and application of the Pediococcus pentosaceus PP90, the Pediococcus pentosaceus PP90 is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.32034. The Pediococcus pentosaceus PP90 can be used for preventing and treating alcoholic nerve injury. The fermentation product of the pediococcus pentosaceus PP90 can degrade the alcoholic strength of alcohol, can generate ethanol dehydrogenase and acetaldehyde dehydrogenase, can improve the activity of nerve cells, can regulate the expression of cytokines, can inhibit the activation of astrocytes, can regulate the expression of brain-derived neurotrophic factors, and can inhibit the growth of the brain-derived neurotrophic factors. The compound can improve the disordered neurotransmitter level, and can be used for preparing products for preventing or improving alcoholic nerve injury.

The invention relates to an enzyme reagent, glucose electrochemical test paper and a preparation method thereof, and belongs to the technical field of electrochemical detection. The glucose electrochemical test paper comprises an electrode substrate and an enzyme reagent arranged on the electrode substrate, the enzyme reagent is prepared from the following components in parts by mass: 5 to 7 parts of buffer solution, 0.01 to 0.2 part of hexamine ruthenium trichloride, 0.01 to 0.1 part of quinone compound, 0.1 to 1.2 parts of metal organic framework immobilized glucose dehydrogenase and 0.2 to 0.8 part of film-forming agent. According to the glucose electrochemical test paper, the detection accuracy of glucose can be improved, and the problems of large interference, small linear range, insensitive detection and the like in the prior art are improved or solved.

The invention relates to the field of pesticide bactericides, and discloses a difluoromethyl thiazole amide-containing compound and application thereof as a bactericide. The difluoromethyl thiazole-containing amide compound has a structure as shown in a formula (I), and the difluoromethyl thiazole-containing amide compound with efficient activity on diseases such as rice sheath blight disease is designed by introducing a difluoromethyl thiazole-containing structure and a diphenyl ether fragment. The compound can be used as a brand new succinate dehydrogenase inhibitor or bactericide. # imgabs0 #

The invention relates to a recombinant pichia pastoris strain for producing astaxanthin as well as a construction method and application of the recombinant pichia pastoris strain. The recombinant pichia pastoris strain is obtained by expressing dehydrogenase Zwf1, phosphate dehydrogenase Gnd2, NADH kinase Pos5 and fusion protein CrtZ-Linker-CrtW of beta-carotene ketolase CrtW and beta-carotene hydroxylase CrtZ in host bacteria. Wherein the host bacterium is a pichia pastoris gene modified strain PP-L. The fermentation conditions of the recombinant strain are optimized based on cofactor engineering, and the astaxanthin production capacity of the pichia pastoris is further improved. The construction method of the recombinant pichia pastoris is simple, synthesis of astaxanthin can be better promoted, the engineering strain can efficiently synthesize astaxanthin by using glucose and methanol through fermentation optimization, and industrial production is facilitated.

The invention discloses an alcohol dehydrogenase mutant and application of the alcohol dehydrogenase mutant in asymmetric synthesis of (S)-6-hydroxy-8-chloro ethyl caprylate with optical purity. The alcohol dehydrogenase or the mutant thereof provided by the invention has extremely high conversion rate and can effectively improve the optical purity of the product. Moreover, based on the mutant, low-cost, efficient and rapid production of single-configuration ethyl 6-hydroxy-8-chloro caprylate can be realized, a multi-configuration mixture is not generated, and the problems of tedious steps, pollution and the like caused by a conventional chemical synthesis method are also avoided.

The invention discloses a formate dehydrogenase mutant and application of the formate dehydrogenase mutant in catalysis of carbon dioxide reduction, the 223 arginine (Arg) of an amino acid sequence of formate dehydrogenase PsFDH48 derived from Paracoccus sp. MKU1 is mutated into proline (Pro) or the 242 proline (Pro) is mutated into phenylalanine (Phe) through a site-specific mutagenesis technology, and the obtained mutants R223P and P242F have higher catalytic activity and can be applied to catalysis of carbon dioxide reduction. The catalyst can catalyze CO2 to generate formic acid more effectively in vitro, and has potential application value in the aspects of efficient activation of CO2 and further conversion of CO2 into other mono-carbon compounds through cascade reaction.

The invention discloses an inositol dehydrogenase mutant and a preparation method of D-chiral inositol, and belongs to the technical field of genetic engineering. The inositol dehydrogenase mutant is obtained by mutating an amino acid sequence as shown in SEQ ID NO.2 through the following mutations: the 200th amino acid is mutated into C from V, the 234th amino acid is mutated into Q from V, and the 256th amino acid is mutated into E from R. According to the invention, the amino acid sequence of wild type inositol dehydrogenase is mutated to obtain the inositol dehydrogenase mutant which is a high-activity mutant capable of catalyzing conversion of myo-inositol into 2-keto-myo-inositol; therefore, when the inositol dehydrogenase mutant and keto isomerase act together to prepare D-chiral inositol by taking myo-inositol as a substrate, the yield of the D-chiral inositol is effectively improved.

The invention discloses a heme oxygenase mutant and bilirubin efficiently synthesized by a multienzyme coupling system of the heme oxygenase mutant, and belongs to the field of biological catalysis engineering. The invention provides a heme oxygenase mutant which is used for catalyzing heme to synthesize biliverdin. Coupling biliverdin reductase to catalyze biliverdin to synthesize bilirubin; cytochrome P450 reductase is introduced to provide electrons; removing competitive binding of the by-product to the substrate heme by carbon monoxide dehydrogenase; formate dehydrogenase is used for in-situ regeneration of coenzyme, and bilirubin is efficiently synthesized through a one-pot method. According to the heme oxygenase mutant, the two core enzymes are coupled with three external assistant enzymes, recombinant escherichia coli is constructed, heme is catalyzed, exogenous NADPH does not need to be added, and the yield of bilirubin within 4 h reaches 1611 mg / L and reaches the bilirubin synthesis level which is reported at present and has the highest yield and the shortest time. According to the technology, resource limitation is relieved, the synthesis method is easy to operate and high in yield, and efficient and green synthesis of bilirubin is achieved.

The invention relates to the field of biochemical engineering, in particular to an alcohol dehydrogenase mutant and application thereof. The mutant is obtained by mutating alcohol dehydrogenase with an amino acid sequence encoding SEQ ID NO: 4 at one or more sites selected from 62nd, 108th, 130th, 132, 134, 182nd, 206th, 287th, 303th, 313th and 314, and the mutation is that the amino acid at the site is mutated into one of A, G, V, L, I, P, F, Y, W, S, T, C, M, N, Q, D, E, K, R and H. According to the alcohol dehydrogenase mutant and the application of the alcohol dehydrogenase mutant, the feeding amount of substrates is increased; the high conversion rate (gt; 99%) of the substrate is realized; according to the present invention, the new YqjM has the good product ee value (gt; 98%), and it is determined that the YqjM derived from the bacillus subtilis old yellow enzyme (belonging to ketene reductase) has the optimal catalytic activity, such that the good industrial application performance is provided.

The invention discloses a biological catalytic synthesis method of norambrox ether, which comprises the following steps: step 1, starting from trans-nerolidol, carrying out carroll rearrangement reaction to synthesize farnesyl acetone; 2, in the presence of a cofactor, converting farnesyl acetone into high farnesol acetate under catalysis of Bayer-Villiger monooxygenase; step 3, catalyzing the deacetylation of the high-farnesol acetate by using esterase to obtain EE-high-farnesol; and step 4, carrying out enzymatic conversion on farnesol by using the squalene hopaene cyclase mutant to obtain norambrox ether. The transformation process is safe and non-toxic, Bayer-Villiger monooxygenase which is new with a key enzyme, natural co-expressed esterase, alcohol dehydrogenase and squalene and hopaene cyclase mutants matched with the system are used for synergistic transformation, so that not only is the coenzyme for oxidation reaction promoted to be recycled, but also the conversion efficiency is improved; conversion from farnesyl acetone to norambrox ether can be efficiently and specifically catalyzed, and a final product can be directly obtained from a reaction system without separation and purification of an intermediate.

The invention relates to the technical field of genetic engineering, and discloses a genetically engineered bacterium for producing L-arginine as well as a construction method and application of the genetically engineered bacterium. Escherichia coli is taken as a starting strain, and the following treatment is performed: overexpressing genes gdhA and gdh of glutamate dehydrogenase, promoting generation of glutamic acid, overexpressing and coding arginine transporter gene argO to enhance extracellular transport of arginine, introducing exogenous argJ to replace a linear synthesis pathway in large intestines, improving the utilization rate of acetyl coenzyme A and accelerating the synthesis efficiency of arginine; a glutamine synthetase gene glnA is over-expressed, and glutamine synthesis is enhanced; the pckA is overexpressed to promote ATP synthesis; meanwhile, speA and adiA genes for coding the arginine decarboxylase are deleted, so that further synthesis of putrescine from L-arginine is inhibited, and the L-arginine accumulation capability of the strain is improved. Through the combined action of the treatment, a genetically engineered bacterium with extremely high L-arginine production yield is finally obtained, and the shake flask fermentation yield can reach 13.7 g / L.

Certain C. glutamicum strains overexpress genes coding for enzymes having the function of aspartate-semialdehyde dehydrogenase, aspartate aminotransferase, dihydrodipicolinate synthase, dihydrodipicolinate reductase, diaminopimelate decarboxylase, aspartatokinase and diaminopimelate dehydrogenase. The C. glutamicum strains also express NAD(P)(+) transhydrogenase subunit alpha PntA and NAD(P)(+) transhydrogenase subunit beta PntB of Corynebacterium urealyticum. A method is developed for the fermentative production of L-lysine using such C. glutamicum strains.

The invention discloses a medicinal and edible anti-alcoholism composition and a preparation method thereof, and relates to the crossing field of biotechnology and food and medicine, the composition comprises the following components: dried orange peel powder, vitamin C, glutathione, phellinus igniarius extract, polygonatum sibiricum extract, oriental wormwood extract, isatis root extract, schisandra chinensis extract, ginseng powder, lucid ganoderma extract and licorice extract. Silymarin, puerarin, white tomato powder, watermelon powder, fucoidin, tomato powder, mung bean powder, celery powder, turmeric powder, hawthorn fruit powder, honey powder, corn peptide, soybean peptide powder, xanthan gum and stevioside are added. The composition provided by the invention has the effect of increasing the content of ethanol dehydrogenase and acetaldehyde dehydrogenase in blood after drinking, and has the capability of promoting alcohol metabolism.

The invention belongs to the technical field of biology, and particularly relates to a method for producing xylitol through two-step enzyme catalysis of hemicellulose hydrolysate, and the method comprises the following steps: taking hemicellulose hydrolysate as a raw material, adding arabinose dehydrogenase, NADH oxidase and coenzyme NADH, and carrying out first-step fermentation until no L-arabinose residue exists in fermentation liquor to obtain fermentation reaction liquor I; and adding xylose reductase and glucose dehydrogenase into the fermentation reaction liquid I, and carrying out second-step fermentation to obtain a fermentation product containing xylitol. According to the two-step enzyme catalysis method provided by the invention, xylitol is prepared, and high-purity xylose does not need to be used as a raw material; according to the present invention, the cheap hemicellulose hydrolysate can be directly adopted as the raw material, the xylose conversion rate and the xylitol yield can exceed 99%, the impurity arabitol content in the product is low, the other impurity saccharic acid is easy to separate, and the problems of complex process, large separation difficulty and high cost in the xylitol refining process are successfully solved.

The invention discloses a 3-ketosteroid-delta1-dehydrogenase mutant and application thereof in synthesis of prednisone acetate, the mutant with high catalytic activity on cortisone acetate is obtained through screening, and a methanol-water phase (Tris-HCl buffer solution) system is adopted to increase the solubility of a substrate so as to improve the biological catalytic efficiency. In a single water phase system (Tris-HCl buffer solution) without methanol, the conversion efficiency is only 64.8% for 60g / L of cortisone acetate, and under the condition that other conditions are the same, the conversion rate in the Tris-HCl buffer solution system with methanol is increased to 92.1%, and the conversion rate is increased by 1.42 times.

The invention relates to the technical field of biology, in particular to a recombinant expression vector, clostridium aerovorans engineering bacteria with micro-aerobic tolerance and a construction method and application of the clostridium aerovorans engineering bacteria. Functional elements of the recombinant vector are all from clostridium ethanolicum, the recombinant expression vector comprises a first recombinant expression vector and a second recombinant expression vector, and the first recombinant expression vector comprises a molecular chaperone CaDnaK as shown in SEQ ID NO.1 and a carbon monoxide dehydrogenase mutant as shown in SEQ ID NO.2 which are expressed in series; the second recombinant expression vector comprises formate dehydrogenase CaFdhF as shown in SEQ ID NO.3 and a hydrogenase mutant as shown in SEQ ID NO.4, wherein the formate dehydrogenase CaFdhF and the hydrogenase mutant are expressed in series; the recombinant expression vector is introduced into clostridium aerovorans, so that the viability of the strain and the conversion efficiency of a gas substrate in a micro-aerobic fermentation environment can be comprehensively improved, the industrial fermentation deoxygenization cost is reduced, and finally, the synthesis gas fermentation income is increased.

The invention discloses alcohol dehydrogenase and a method for preparing (R)-6-hydroxy-8-chloro ethyl caprylate through catalysis of the alcohol dehydrogenase, and the alcohol dehydrogenase is selected from one or more of mutants of ADH1, ADH2, ADH3, ADH4, ADH5, ADH6, ADH7, ADH8, ADH9, ADH10 and ADH10; the amino acid sequences of the ADH1-ADH10 are as shown in SEQ ID NO. 1 to SEQ ID NO. 10. According to the invention, ethyl 6-carbonyl-8-chloro caprylate can be catalyzed to generate ethyl (R)-6-hydroxy-8-chloro caprylate or ethyl (S)-6-hydroxy-8-chloro caprylate, the reaction conversion rate of the mutant ADH10-A184L / N87F / A61L of ADH10 reaches 99%, and the e.e. Value of the R type is 99.2%.

The invention provides a formate dehydrogenase mutant with improved electron transfer rate and application thereof, the formate dehydrogenase mutant takes an amino acid sequence as shown in SEQ ID NO.1 or an amino acid sequence having at least 80%, 85%, 90%, 95%, 97%, 98% or 99% identity with the SEQ ID NO.1 as a parent, and has site-saturated mutation corresponding to tyrosine at the 262nd site of the SEQ ID NO.1. The TsFDH mutant with improved electron transfer rate and stable catalytic activity is finally obtained by performing site-directed saturated mutation on formate dehydrogenase from thiobacillus.

The invention provides application of a lactobacillus mucus fermentation strain A21196 in preparation of a product for promoting ethanol metabolism, and belongs to the technical field of microbial preparations. The lactobacillus mucus fermentation strain A21196 has the high-yield ethanol dehydrogenase and acetaldehyde dehydrogenase, and has the effects of reducing the content of ethanol and acetaldehyde in the blood of mice suffering from acute alcoholism and shortening the central nervous inhibition time in vivo. Therefore, the invention provides the application of the fermented lactobacillus mucus strain A21196 in preparation of a product for preventing and / or treating the alcoholic liver injury or the alcoholic liver disease.

The invention discloses a Lactobacillus paracasei grx06 and an application thereof in the aspects of dispelling the effects of alcohol and protecting the liver, the strain number of the Lactobacillus paracasei is grx06, the Lactobacillus paracasei is preserved in the China General Microbiological Culture Collection Center on February 18, 2025, and the preservation number is CGMCC No: 33560. The lactobacillus paracasei obtained by screening in the invention has the capability of producing ethanol dehydrogenase and acetaldehyde dehydrogenase at high yield, and is high in ethanol tolerance. In addition, the traditional Chinese medicine composition has the effects of promoting ethanol metabolism in rats and recovering liver injury. Wide application prospects are realized in the field of anti-alcohol and liver-protecting products.

The present disclosure provides polymorphs of 3-(2,3,5,6-tetrafluoro-3′-trifluoromethoxy-biphenyl-4-ylcarbamoyl)-thiophene-2-carboxylic acid, and methods of making such polymorphs. The present disclosure further provides pharmaceutical compositions and kits comprising polymorphs of the invention, and methods of treating, preventing, or ameliorating a disease or condition comprising administering a polymorph of the invention.

The invention provides a construction method and application of recombinant yarrowia lipolytica with high yield of tyrosol, and belongs to the field of bioengineering. The recombinant yarrowia lipolytica with high yield of tyrosol is named as a yarrowia lipolytica XJ-TL strain, and the recombinant yarrowia lipolytica with high yield of tyrosol is named as a yarrowia lipolytica XJ-TL strain. The recombinant yarrowia lipolytica disclosed by the invention expresses an ethanol dehydrogenase encoding gene, and simultaneously expresses any one or a combination of more of a DAHP synthase mutant encoding gene, a chorismate mutase mutant encoding gene, a glucose 6-phosphate dehydrogenase encoding gene, a transketolase encoding gene and a transaldolase encoding gene; meanwhile, a natural promoter of a pyruvate kinase coding gene is truncated. After the recombinant yarrowia lipolytica XJ-TL strain is subjected to fed-batch fermentation, tyrosol can be synthesized at a relatively high level. The construction method of the recombinant yarrowia lipolytica is easy to operate, and efficient synthesis of tyrosol can be achieved.

The invention discloses a recombinant pichia pastoris strain with high yield of retinaldehyde as well as a construction method and application of the recombinant pichia pastoris strain. The recombinant pichia pastoris strain is obtained by expressing dehydrogenase Zwf1, phosphate dehydrogenase Gnd2, NADH kinase Pos5 and two copied beta-carotene-15, 15 '-monooxygenase BCMO in host bacteria. Wherein the host bacterium is a pichia pastoris gene modified strain PP-B (Propene Polymer-B). The retinaldehyde production performance of the recombinant strain is verified based on cofactor engineering and the copy number of key enzymes, and the retinaldehyde production capacity of the pichia pastoris is further improved. The recombinant pichia pastoris disclosed by the invention is simple in construction method, can better promote the synthesis of retinol, and is beneficial to industrial production as the engineering strain can efficiently synthesize retinol by using methanol as a unique carbon source through amplification fermentation in a 5L fermentation tank.

The invention belongs to the field of biosynthesis, and particularly relates to a saccharomyces cerevisiae engineering bacterium for producing glycosyl donor UDP-arabinose as well as a construction method and application of the saccharomyces cerevisiae engineering bacterium. According to the invention, UDP-Glc dehydrogenase, UDP-GlcA decarboxylase, UDP-Xyl-4-epimerase or UDP-Glc-epimerase are sequentially expressed in WAT11, so that a synthetic route of UDP-Ara is introduced into the saccharomyces cerevisiae for the first time; de novo synthesis of Ara-oleanolic acid is realized by introducing a synthetic route of arabinosyl transferase and oleanolic acid; the yeast containing the UDP-Ara synthetic pathway and the arabinosyl transferase gene can also utilize betulinic acid to generate Ara-betulinic acid. The invention solves the problem of yeast total synthesis of the arabinosyl donor, provides a new biotechnology platform for production of natural glycoside compounds, and has important theoretical value and wide application prospect.

The invention relates to pediococcus acidilactici PA27 capable of degrading alcohol and improving alcoholic nerve injury and application of the pediococcus acidilactici PA27, the pediococcus acidilactici PA27 is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.31922. The pediococcus acidilactici PA27 can degrade alcohol and improve alcoholic nerve injury. The pediococcus acidilactici PA27 separated and screened by the invention can grow and propagate in an alcohol-containing culture medium, can degrade alcohol and can generate ethanol dehydrogenase and acetaldehyde dehydrogenase; the polypeptide can be used for constructing a blood-brain barrier and reducing the damage of alcohol to central nervous cells; the traditional Chinese medicine composition can reduce the expression of inflammatory cytokines TNF-alpha, inhibit the activation of astrocytes, promote the expression of brain-derived neurotrophic factors BDNF, regulate the release of alcohol-induced astrocyte neurotransmitters and improve alcoholic nerve injury.

The invention discloses an enzyme catalytic synthesis method of a luliconazole chiral intermediate, which comprises the following steps: adding a recombinant escherichia coli wet cell for expressing alcohol dehydrogenase, a recombinant escherichia coli wet cell for expressing formate dehydrogenase, a buffer solution, a substrate, a cosolvent, ammonium formate and a coenzyme into a reaction container, reacting at 25-35 DEG C for 2-24 hours, extracting, separating, and carrying out rotary evaporation, thereby obtaining the luliconazole chiral intermediate. The luliconazole chiral intermediate is obtained; the substrate is 2, 2 ', 4'-trichloroacetophenone; the alcohol dehydrogenase is a mutant of AKR3. The method is mild in reaction condition, environmentally friendly, high in regioselectivity and stereoselectivity, high in conversion rate, high in chiral purity, small in enzyme dosage, low in preparation cost and suitable for industrial production.

The invention relates to the technical field of serum specimen typing identification, in particular to a serum specimen typing identification system based on patient centralized detection. Comprising a data acquisition module, a data processing module, a data analysis module and a typing identification module, the data acquisition module is used for collecting and recording serum index data of a patient; according to the method, serum index data, including C-reactive protein, glucose, lactic dehydrogenase, TNF-alpha, IgG and IgM, of the patient are collected, an index matrix is constructed, and homogenization treatment is performed, so that a serum index distribution map of the patient is formed, the relationship between serum index values of the patient is conveniently and intuitively reflected, the synergistic reaction effect of each index on inflammation is reflected, and the sensitivity of the patient is improved. Meanwhile, a serum specimen of a new patient is subjected to typing, disease indexes are calculated, and medical staff can conveniently carry out targeted treatment on the patient.

A recombinant yeast cell that functionally expresses: —a nucleic acid sequence encoding a protein having glycerol dehydrogenase activity; —a nucleic acid sequence encoding a protein having dihydroxyacetone kinase activity; and —a nucleic acid sequence encoding a protein having glycerol transporter activity, wherein the expression of the nucleic acid sequence encoding the protein having glycerol transporter activity is under control of a promoter (the “GT promoter”), which GT promoter has an anaerobic / aerobic expression ratio for the glycerol transporter of 2 or more, and a process for the production of ethanol using such recombinant yeast cell.