82 results about "Candida lipolytica" patented technology

The invention relates to a group of alive microbial preparations for preparing compound microbial fertilizer and the preparation method thereof. The alive microbial preparations include a microbial inoculant and an organic material degradation agent. The microbial inoculant includes Bacillus megaterium, Bacillus licheniformis, Bacillus mucilaginosus, Bacillus laterosporus, Azotobocter chroococcum, etc. The organic material degradation agent includes Bacillus megaterium, Bacillus licheniformis, Bacillus subtilis, Bacillus circulans, Saccharomyces cerevisiae, Candida lipolytica, Bacillus pumilus, Bacillus cereus, Streptomyces griseus, Pseudomonas alcaligenes, Pseudomonas cepacia, Streptococcus salivarius subsp. Thermophilus, Geobacillus stearothermophilus, Streptomyces Thermophilus, Geotrichum candidum and Aspergillus oryzae. The preparation method comprises the following steps: carrying out submerged fermentation, directly mixing the bacterial liquids and stirring to obtain a liquid preparation; or mixing with an absorbent to obtain a solid preparation. The alive microbial preparations have the advantages of improving soil fertility, promoting plant growth, enhancing anti-disease, anti-insect and anti-drought capacities of plants, improving quality of agricultural products and so on.

The present invention relates to microbial fermentation technology, and is especially Candida lipolytica and the erythritol producing process therewith. The erythritol producing process includes the following steps: 1. fermentation in glucose containing culture medium with Candida lipolytica for producing erythritol in high yield; and 2. separating and purifying erythritol from the fermented liquid.

The invention discloses composite bacteria for removing oil from kitchen waste and a preparation method for the composite bacteria. The preparation method comprises the following steps of: performing high-density culture on strains of trichoderma viride, bacillus subtilis, black rhizopus, photosynthetic bacteria, candida lipolytica, bacillus licheniformis, nitrosomonas europaea and nitrobacter winogradskyi respectively to obtain microbial dry hypopus powder; and mixing 15 to 20 weight parts of trichoderma viride, 10 to 15 weight parts of bacillus subtilis, 25 to 40 weight parts of black rhizopus, 5 to 10 weight parts of photosynthetic bacteria, 5 to 10 weight parts of candida lipolytica, 10 to 15 weight parts of bacillus licheniformis, 5 to 15 weight parts of nitrosomonas europaea and 5 to 15 weight parts of nitrobacter winogradskyi to obtain the composite bacteria for removing the oil from the kitchen waste. The composite bacteria can quickly decompose lipid ingredients in the kitchen waste when used for treating the kitchen waste; and compared with the prior art, the method has the advantages that: the production investment and the production cost are low, and the method is convenient to use.

The invention discloses a candida lipolytica strain and a method for preparing long chain dicarboxylic acid by using the candida lipolytica strain, belonging to the field of microbial fermentation. The candida lipolytica strain is candida lipolytica CX-998 CCTCC NO:M2011094. The method for preparing the long chain dicarboxylic acid comprises the following steps of: in a culture medium in which C10-C18 n-alkane is used as a substrate, using the candida lipolytica strain to perform fermentation; and separating, extracting and recycling the fermentation product to obtain the long chain dicarboxylic acid which is the same as an n-alkane substrate chain. According to the method, all sorts of single and mixed binary acids of C10-C18 can be produced, wherein in a 10-liter fermentation tank, when DC11 is produced from nC11 through fermentation transformation, the DC11 output in 144 hours reaches 165g/L, and the after-treatment yield is 88%; and when the purity of the nC11 is more than 99%, the purity of the DC11 reaches 99%.

The invention discloses novel bean water biological power fermentation liquor and a preparation method thereof. The novel bean water biological power fermentation liquor comprises the following raw materials of soybeans, water and probiotics, wherein the probiotics comprise the following components of candida lipolytica, bifidobacterium adolescentis, lactobacillus bulgaricus, bifidobacterium bifidum, lactobacillus acidophilus and lactococcus lactis. The novel bean water biological power fermentation liquor has the beneficial effects that the novel bean water biological power fermentation liquor is prepared by adding the probiotics to bean water, and the novel bean water biological power fermentation liquor contains rich trace elements of copper, phosphorus, ferrum, potassium, calcium, zinc, molybdenum and the like, and further contains flavone, saponin, folic acid, vitamins, 16 amino acids necessary for human bodies, and rich probiotics; the power fermentation liquor as a raw materialcan be made into foods, a biological feed, a biological fertilizer, environmental-friendly fermentation liquor and the like, can provide rich nutrient components, and can also effectively decompose the nutrient substances of other raw materials, absorption is facilitated, the conversion rate of the raw materials can be increased, and pollution to environment can be reduced.

The invention belongs to the environment-friendly field, and discloses a method for improving oil sludge separating and processing efficiency and a bacterial strain used for the method. The method for improving the oil sludge separating and processing efficiency comprises the following steps: (1), sampling and analyzing processed oil sludge; (2), determining microorganism nutrients to be added according to components and content of a nitrogen element and a phosphor element in the processed oil sludge; (3), adding a mixed culture of candida lipolytica Y-57 and pseudomonas putida P-101 and the microorganism nutrients into the to-be-processed oil sludge, stirring, ventilating and culturing for over 8 hours under the condition with pH of 6-10 under a temperature of 8 DEG C-45 DEG C to separate oil and mud in the oil sludge. According to the invention, a microorganism treatment method is adopted for separating the oil and the mud in the oil sludge to achieve an effect of an oil resource recovery rate; moreover, a chemical agent which generates influence to the environment is not added, so that secondary pollution is not generated. Resourceful treatment is carried out on oil-containing mud to obtain a certain economic benefit, and therefore, the method is an economical and effective oil sludge treating technology.

The invention discloses a domestic sewage treatment microbial inoculant which comprises polyacrylamide, polyethyleneimine, silicon dioxide and compound microorganisms, wherein the compound microorganisms include Bacillus subtilis, Pseudomonas aeruginosa, Bacillus cereus, Candida lipolytica, nitrobacteria and Paracoccus denitrificans. The treatment agent can effectively treat domestic sewage, is low in cost and favorable in effect, and reduces pollution of the domestic sewage to the ecological environment; the sewage suspended substance removal rate is up to 99.8%, the BOD (biochemical oxygen demand) removal rate is 98%, the COD (chemical oxygen demand) removal rate is 98.6%, the chroma is 5, and the sludge quantity is so low that no sludge treatment system needs to be arranged; and the domestic sewage treatment microbial inoculant causes no secondary pollution, and is stable in treatment effect, environment-friendly and non-toxic.

The invention discloses an industrial sewage treatment agent, comprising polyacrylamide, silicon dioxide, peroxysulphate, sodium carbonate and compound microorganisms, wherein the compound microorganisms comprise bacillus licheniformis, pseudomonas aeruginosa, bacillus cereus, candida lipolytica, nitrifying bacteria and paracoccus denitrificans. By adopting the industrial sewage treatment agent disclosed by the invention, the removal rate of sewage suspension solids can be up to 99.8%, the biochemical oxygen demand (BOD) can be up to 98.7%, the chemical oxygen demand (COD) removal rate can be up to 98%, the industrial sewage treatment agent is less in sludge amount, free of secondary pollution, stable in treatment effect, environment-friendly, and non-toxic.

The invention discloses a method for producing erythritol. Glucose is taken as raw material, candidalipolytica is taken as a fermentation strain, shaking culture as well as first-stage and second-stage inoculum development are performed, then the fermentation in a fermentation tank is carried out, mycoprotein impurities in zymotic fluid are removed by a ceramic membrane after the fermentation is finished, then a sodium membrane is used to selectively pass through the erythritol so as to extract the erythritol, and finally the erythritol product is obtained through condensation crystallization and separation drying. The method has the advantages of short incubation period, high extraction yield, low production cost and so on.

The invention discloses an efficient sewage treatment microbial agent. The efficient sewage treatment microbial agent is prepared from, by weight, 2-4 parts of nitrosobacteria, 10-20 parts of bacillus subtilis, 8-15 parts of alcaligenes faecalis, 5-10 parts of bacillus megatherium, 2-8 parts of pseudomonas aeruginosa, 2-10 parts of candida lipolytica, 2-8 parts of deoxidized paracoccus, 2-6 parts of pseudomonas stutzeri, 2-10 parts of saccharomyces cerevisiae, 1-4 parts of aspergillus niger, 0.1-0.6 part of yeast extract, 2-6 parts of peptone, 0.1-0.5 part of glucose, 8-15 parts of kaolin, 2-5 parts of aluminum polychlorid, 0.1-0.5 part of FMEE and a proper amount of water. The efficient sewage treatment microbial agent is suitable for treating industrial waste water, breeding waste water and the like, all strains are in cooperation, symbiotic coordination is achieved, high activity is achieved, and reproduction is fast. The efficient sewage treatment microbial agent is put in the waste water to be treated, the degradation effect can be effectively accelerated, the treatment water quality can be greatly improved, operating cost can be reduced, the treated water reaches the standard to be drained, and damage to environment is reduced.

The invention provides a method for producing organic fertilizer from kitchen waste. The method comprises the steps: selecting two kinds of bacteria and two kinds of saccharomycetes, respectively preparing culture media and seed solutions, and transferring the bacteria and the saccharomycetes into fresh slant culture mediums respectively, so as to carry out activation respectively; respectively putting the activated strains into agar-removed culture mediums, culturing the bacteria for 8-12 hours at the temperature of 35-39 DEG C, and culturing the saccharomycetes for 15-19 hours at the temperature of 30-34 DEG C, so as to obtain seed solutions, wherein the revolving speed of each shaking table is 150-250r/min; inoculating a bacillus amyloliquefaciens seed solution, a bacillus cereus seed solution, a candida lipolytica seed solution and a candida tropicalis seed solution, which are in the ratio of (2-3) to (2-3) to (1-2) to (1-2), to the kitchen waste according to the mass ratio of 8-12%, and fermenting, thereby obtaining the organic fertilizer. According to the method, a biodegradation technology is utilized, the operation is simple and convenient, the energy consumption is low, the safety is good, no off flavors or peculiar smells exist, and no secondary pollution is caused.

The invention relates to a method for producing liquid organic bacterial manure by utilizing the waste liquor of restaurants and kitchens. The method comprises the following steps of: selecting bacillus megaterium, candida lipolytica and pseudomonas; preparing a pseudomonas slope culture medium, a bacillus megaterium slope culture medium and a candida tropicalis slope culture medium; conveying the pseudomonas, the candida lipolytica and the bacillus megaterium into the fresh slope culture mediums respectively, and culturing at the temperature of 30 DEG C for 24 hours and activating; putting the activated strains into a culture medium in which agar is removed respectively, and culturing at the temperature of 30 DEG C for 18 hours at the rotation speed of 180 revolutions/minute of a shaker to obtain activated bacterial liquid, wherein the inoculation amount is 2 percent; inoculating the activated bacterial liquid of the pseudomonas, the bacillus megaterium and the candida lipolytica into the rubbish waste liquor of restaurants and kitchens in a ratio of 1:1:1, wherein the inoculation amount is 2 percent; adding 60 percent excrement, which serves as auxiliary materials, of livestock and poultry to prepare culture expanding bacterial liquid; and fermenting the compounded bacterial liquid at the temperature of 30 DEG C for 72 hours to obtain the liquid organic bacterial manure.

The invention discloses a fermentation method for producing erythritol. The fermentation method comprises the following steps that candida lipolytica strains are activated and subjected to shake culture and first-grade and second-grade seed enlarged culture; citric acid is dissolved with water, ammonium hydroxide is added, and ammonium citrate is prepared; an organic nitrogen source, inorganic salt and water are added into a nutritive salt tank for dissolving and evenly stirred; ammonium citrate is injected into the nutritive salt tank and mixed with nutritive salt, and the mixture is evenly stirred and heated through steam for sterilization; a glucose solution is injected into a fermentation tank and heated through steam for sterilization; a second-grade seed solution and nutritive salt mixed solution is injected into the fermentation tank and is evenly mixed with the sterilized glucose solution; the stirring rotating speed of the fermentation tank is set, compressed air is introduced, the temperature is well controlled, and fermentation is started; then, the stirring rotating speed is decreased, the air introducing amount is increased, and the fermentation temperature is well controlled; fermentation is ended with glucose is smaller than or equal to 0.1 g/100 ml. By means of the fermentation method, the problems that an existing method is low in conversion rate of erythritol and long in production period are solved, and the fermentation method has the advantages that the operation cost is low, the fermentation period is short, and the yield of erythritol is high.

The invention is a highly active protein, taking multiple agricultural and sideline products as raw materials, inoculating multiple bacterial strains and adopting liquid-solid combined fermenting process to make it. Its raw materials includes: bran, aginomoto residue, beer residue, vermicelli protein flour, and corn protein flour; and the fermenting bacterial strains: lipolytic Candida, rice aspergillus, deproteinizing Candida, black mold and Geotrichum candidum. The multi-bacterial strain liquid- solid combined fermenting process to produce highly active protein has a flow: (1) inclining bacterial strains; (2) shaking bottle to culture; (3) culturing in fermentation cylinder; (4) weighing, mixing and adding water of raw materials; (5) curing raw materials; (6) inoculating liquid bacterial strains; (7) solid-fermenting; (8) drying and crushing; (9) measuring and packaging. The highly active protein has high amino acid content, especially the number of two most important ones-lysine and methionine and that of yeast active cells are large, therefore the proportion of it replacing import fish protein concentrate can be increased from <40% to 60-80%.

The invention relates to the technical field of microbes, in particular to method for producing ribonucleic acid by high-density continuous culture of high-nucleic-acid-yield Candida lipolytica in a stirring tank, which is characterized in that by comprising: activating a strain with a collection number of CGMCC No.3558 in a slant culture medium and culturing the strain in a shake flask; and fermenting the strain obtained in the shake flask in the stirring fermentation tank and extracting and separating to obtain ribonucleic acid, wherein in the stirring fermentation tank, intermittent fermentation is performed in a fermentation medium first, high density fermentation culture is performed 3 to 8 hours later under the conditions of constantly adding a supplemented medium a and a supplemented medium b, and when the concentration of dry bacteria reaches 33 to 36g/L, a high-density continuous fermentation culture medium is added batch to perform high-density continuous fermentation. The method improves the bacteria and RNA yield in unit fermentation tank volume greatly, lowers energy consumption and reduces discharge. The process lowers labor intensity and simplifies a production process.

The invention belongs to the technical field of catering wastewater treatment, and particularly relates to a microbial agent for the treatment of catering oily wastewater and a treatment method thereof. The microbial agent includes fungi and bacteria and, the fungi include any one or more combinations of Mucor roxianus, Mucor racemosus, Aspergillus oryzae, Aspergillus niger, Candida lipolytica, Candida tropicalis and Saccharomyces cerevisia; and the bacteria include any one or more combinations of Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus pumilus, Rhodopseudomonas palustris, Rhodopseudomonas sphaeroides, Thiobacillus ferrooxidans and Streptococcus thermophilus. The degradation rate of the microbial agent to oils and fats can reach 90% or above.

The invention relates to the field of biological medicine, in particular to a Candidatropicalis strain for producing ribonucleic acid with high yield and an application thereof. The Candida lipolytica strain of the invention is submitted to be preserved at China General Microbiological Culture Collection Center on December 30th, 2009, and the preserving number is CGMCCNo.3558. The strain of the invention not only has high content of nucleic acid but also has the characteristic that paraffin oil can be used by Candida lipolytica as a carbon source to be applied to industrialized production of nucleic acid.

The invention relates to a method for increasing the erythritol conversion rate through adding mixed magnesium salt. The method comprises the steps of firstly culturing candida lipolytica didd. et lodd. strain liquid, and then performing fermentation culture on the candida lipolytica didd. et lodd. strain liquid so as to obtain the erythritol, wherein fermentation culture mediums include components of 0.3-0.35kg/L of glucose, 4-7g/L of organic nitrogen sources and 5-6g/L of inorganic salt. According to the method disclosed by the invention, part of raw materials of the fermentation culture mediums are replaced, addition of single magnesium salt is changed into addition of mixed magnesium salt, the proportion of the mixed magnesium salt is adjusted, and the fermentation culture mediums areoptimized, so that the strain metabolic pathway more facilitates generation of the erythritol, and the erythritol conversion rate can be effectively increased.

The invention discloses a composite microbial agent for efficiently degrading high-grease kitchen garbage and a preparation method of the composite microbial agent. The composite microbial agent provided by the invention is prepared from aspergillus oryzae powder, bacillus licheniformis powder, candida lipolytica powder, bacillus aceticus powder, bacillus coagulans powder and azotobacter chroococcum powder. By researching compounding of various microorganisms, when the compounding ratio of the aspergillus oryzae powder to the bacillus licheniformis powder to the candida lipolytica powder to bacillus aceticus to bacillus coagulans to the azotobacter chroococcum powder is 2:1:1.2:1.5:2:1 and the inoculation amount is 4%, the degradation rate of the kitchen garbage can reach 92% or above, main components (cellulose, grease, starch and protein) of the kitchen garbage can be effectively degraded, and the kitchen garbage can be fully fermented.

The invention relates to a method for producing liquid organic bacterial manure by using kitchen waste liquid. The method comprises the steps: bacillus megaterium, candida lipolytica and pseudomonas are selected, a pseudomonas slant culture medium, a bacillus megaterium slant culture medium, and a candida tropicalis slant culture medium are prepared, pseudomonas, candida lipolytica, and bacillus megaterium are correspondingly transferred to a fresh slant culture medium and incubate at 30 DEG C for 24 h for activation, activated bacteria are correspondingly placed in an agar-removing medium andare cultured at 30 DEG C for 18 hours, inoculation amount is 2%, table rotating speed is 180 rpm, and activated bacterial liquid is obtained. The activated pseudomonas, bacillus megaterium, and candida lipolytica are inoculated into the kitchen waste liquid in a ratio of 1 to 1 to 1, the inoculation amount is 2%. 60% of livestock and poultry excrement materials are added to prepare culture enlarging bacterial liquid. The compound bacterial liquid is fermented at 30 DEG C for 72 hours to obtain the liquid organic bacterial manure.

The invention relates to the field of microorganisms, and particularly relates to a method for producing erythritol by inoculating in a fermentation tank instead of seed flasks. The method disclosed by the invention comprises the following steps: inoculating candida lipolytica for producing erythritol to multiple eggplant flasks, wherein an inclined culture medium comprises the following components in parts by weight: 150-250 parts of glucose, 5-15 parts of a yeast extract, 0.5-1.5 parts of urea, 15-25 parts of agal-agal, and 800-1200 parts of tap water; after the pH value is adjusted to 6.0, culturing for 45-50 h at a temperature of 28-32 DEG C, and adding 80-120 parts of sterile water in each eggplant flask; and inoculating all lawns obtained by washing to a sterilized fermentation tank containing 20 L of the culture medium to culture for 15-22 h, completely inoculating the obtained objects to a primary seed flask, and then enabling the obtained products to pass through a secondary seed flask and the fermentation tank, so that erythritol is produced. The method disclosed by the invention is designed for producing erythritol by inoculating in the fermentation tank instead of seed flasks, thereby saving manpower and time, and greatly reducing the incidence of bacterial infection.

The invention relates to an electroplating waste water conditioning agent formula. The electroplating waste water conditioning agent formula is characterized by comprising the following raw materialsin parts by mass: 10 to 33 parts of molasses, 0.1 to 0.5 part of nitrogen source, 0.01 to 0.05 part of ammonium sulfate, 0.001 to 0.003 part of candida lipolytica, 5 to 15 parts of saccharomycetes liquid, 0.02 to 0.05 part of dextranase, 0.01 to 0.03 part of protease, 5 to 10 parts of pseudomonas aeruginosa, 2 to 3 parts of humic acid, 2 to 4 parts of bacillus subtilis, 3 to 4 parts of bacillus licheniformis, 1 to 2 parts of Aspergillus niger, and 67 to 90 parts of water. The addition amount of the molasses of the waste water biochemical treatment active sludge conditioning agent provided by the invention is far higher than that of an ordinary yeast culture medium, and the purpose is to hydrolyze macromolecule glucose which is difficult to degrade in the molasses into small-molecular glucose and hydrocarbons while the glucoses are used by the yeast, thereby being more suitable for bacteria in the active sludge to use.