79 results about "Geobacillus" patented technology

This invention relates to selection and application of thermophil denitriding Bacillales,of which conservation number is CGMCC-1228. Initial strain is from water of oil field. The strain is obtained by selection, inoculation, and domestication with good thermal stability. It belongs to soil Bacillales and can be used in fermented industry, which needs thermally stable enzyme. It grows well in the oil field and can degrade paraffin and decrease viscosity to improve liquidity. All of this can increase recovery and carriage efficiently. It can be used to control pollution and prepare surfactant.

Novel strains Paenibacillus sp. BS-0048, Paenibacillus sp. BS-0074, Paenibacillus polymyxa BS-0105 and Paenibacillus sp. BS-0277 and Fusaricidin A, Fusaricidin B and novel compounds 3 and 4 produced thereby have an activity of inducing resistance to plant diseases. Thus, they can protect plants from infections with fungi, bacteria, viruses and so on and, as a result, effectively control plant diseases.

The invention discloses a compound bacterium agent and a method for accelerating fast maturity by inoculating compound bacterium agent to compost. The method comprises the steps of: performing enlargement cultivation respectively on high-temperature microbial strains such as Geobacillus, Bacillus licheniformis, two trichoderma, actinomyces and the like which are screened from the matured compost, and combining the strains in certain proportion into the compound bacterium agent. The compound bacterium agent is inoculated to the compost in certain proportion, the compost is moved into a fermentation tank for aerated aerobic fermentation, and the compost maturity is completed. Compared with the control compost, the inoculated compost of the scheme of the invention reaches the high temperature period one week earlier than the control compost, and the high temperature period lasts for 14 days so as to be beneficial to the decomposition and humification of organic matters of the compost; and the inoculated compost is thoroughly decomposed about twenty days later. Through test, most of the weed seeds, ascarid eggs and colibacillus in the compost are dead, the humic acid content is 25 g / kg higher than that of the control compost, the humification coefficient is more than 1.9, the decomposed finished product is black brown and odourless, and has excellent stability, harmless and minimized quality, apparently environmental and economic benefits.

The present invention relates to thermostable proteinases from thermophilic Bacillus species and their uses in the preparation of nucleic acid samples. The enzymes of the invention are stable and active at 65-80 ° C., but are readily autolysed or denatured above 90° C.

The invention discloses a composite microbe oil displacement agent and the use thereof. The composite microbe oil displacement agent comprises pseudomonas fermentation anaculture and geobacillus fermentation anaculture, and the volume ratio of the pseudomonas fermentation anaculture to the geobacillus fermentation anaculture ranges from 1:10 to 10:1. The composite microbe oil displacement agent is non-toxic and harmless, is easy to degrade, and is an environmental-friendly biological agent; the manufacturing cost is low, and the composite microbe oil displacement agent is easy to manufacture and apply on a large scale; the using temperature range is wide (from 10 to 100 DEG C), and high-viscosity crude oil emulsion can be processed through emulsion breaking effectively; deposits cannot be generated after the composite microbe oil displacement agent is mixed with high hardness groundwater, and the composite microbe oil displacement agent is suitable for oil displacement, plugging removal and emulsion breaking of a low permeability oilfield and an ultra-low permeability oilfield; and the composite microbe oil displacement agent achieves high efficiency of oil displacement and emulsion breaking.

Tools and methods for detecting the presence bacteria, yeast and mold in a sample obtained from a food sample are provided. The methods employ a polymerase chain reaction and primer and probe sets that are based on the 16S rRNA and squalene-hopene cyclase genes of Alicyclobacillus and Geobacillus and the 18S rDNA gene of mold and yeast. The present invention also relates to primer and probe sets. Each primer and probe set comprises a forward primer and a reverse primer, both of which are from 15 to 35 nucleotides in length and a probe.

The invention discloses a method for increasing the starch branching enzyme activity, and belongs to the field of genetic engineering and enzyme engineering. The method comprises the steps that the 349 methionine of a starch branching enzyme derived from Geobacillus thermoglueosidan is mutated into threonine (Thr) or serine (Ser) separately through a site-directed mutation method, the activity of the obtained mutant is obviously increased compared with a wild starch branching enzyme, and industrial production and application of the starch branding enzyme are promoted.

The invention provides a phenylalanine dehydrogenase (PDH) mutant derived from geobacillus, which is high in enzyme activity and thermal stability compared to wild PDH. Furthermore, the invention provides a method for catalytic synthesis of a saxagliptin chiral intermediate, namely (S)-N-t-butyloxycarbonyl-3-hydroxy-1-adamantyl-D-glycine (Boc-HAG) through the PDH mutant. According to the method provided by the invention, Boc-HAG can be directly prepared through a reaction in two steps, and e.e. (enantiomeric excess) value exceeds 99.9%; generation of side products can be reduced, yield of 95% can be achieved within 12 hours, catalysis time is greatly shortened, energy consumption is reduced and a post-treatment process is simplified.

The invention relates to a sulfadimidine degrading bacterium S-07 degraded by microorganisms and a screening method thereof. The sulfadimidine degrading bacterium S-07 is verified as Geobacillus, and the Genbank registration number of the bacterium 16S rDNA is KU588289. The sulfadimidine degrading bacterium is preserved in the China General Microbiological Culture Collection Center on April 21st, 2016, and the preservation number of the sulfadimidine degrading bacterium is CGMCC No. 12382. The sulfadimidine degrading bacterium S-07 well grows within the temperature range of 50-75 DEG C, has quite strong tolerance to sulfadimidine, and can efficiently degrade sulfadimidine in a liquid phase and a solid phase. The bacterium can be used for degrading and removing sulfadimidine residues in livestock excrement or sludge high-temperature compost, the removal rate is larger than 95%, and the sulfadimidine degrading bacterium S-07 has good industrial application prospects and environment benefits.

The invention belongs to the technical field of plant growth-promoting bacteria development, and concretely relates togeobacillus thermodenitrificans TB62 and application thereof in promoting compost maturity. The geobacillus thermodenitrificans TB62 is named as Geobacillus thermodenitrificans in taxonomy, is preserved in Common Microorganism Center of China Committee for Culture Collection of Microorganisms in January 05, 2017, and has the preservation No. CGMCC 13530. The geobacillus thermodenitrficans TB62 provided by the invention is firstly screened and separated from samples during a compost high temperature period, and is high in temperature resistant, fast in growth and propagation, and capable of producing lignocellulose-degrading enzyme; a microbial agent containing geobacillus thermodenitrificans TB62 can be beneficial to improving the compost temperature, accelerating organic matter degradation and water-dissolved organic matter (DOM) degradation, improving the content of Kjeldahl nitrogen, and promoting compost maturity; the geobacillus thermodenitrificans TB62 also has a function of biosurfactant production so as to be applicable for preparing a surface active agent.

To provide a mixture, a dissolving solution and a pharmaceutical agent, which contain a thermophilic microorganism to make it possible to regulate a mucous membrane immune system gene cluster and metabolism-related gene clusters of the intestines and the liver. Each of them is prepared by fermentation of an organic material containing a thermophilic microorganism at a temperature of 50° C. or more and 90° C. or less. By being administered to the animal, it regulates at least one of a mucous membrane immune system gene cluster, a metabolism-related gene cluster in the intestines, and a metabolism-related gene cluster in the liver of the animal. The microorganism includes at least one species of the genus Bacillus, Oceanobacillus, Paenibacillus, Anoxybacillus, Lysinibacillus, Methanopyrus, Geogemma, Pyrolobus, Pyrodictium, Hyperthermus, Pyrococcus, Pyrobaculum, Thermococcus, Aeropyrum, Aquifex, Thermotoga, Thermodesulfobacterium, Thermus, Geobacillus, and Thermomyces.

The invention provides a strain of Geobacillus thermodenitrificans as well as the screening method and the uses thereof. The strain was deposited as the number CGMCC-1228 in the China General Microbiological Culture Collection Center of the China Committee of Culture Collection for Microorganisms. The strain was obtained by primary screening, re-screening, inoculation, domestication and propagation using the bacteria in the aqueous samples from the strata of oil field as the original screening strain. The strain screened in the invention belongs to the genus Geobacillus, which can tolerate a high temperature, and has good thermostability, which can be applied to the industrial production which need the condition of thermostable enzymes, such as fermentation, etc. With its ability of growing well in the oil-reservoir environment, degrading alkanes, decreasing viscosity and increasing fluidity of crude oil, the strain is able to remarkably enhance the yield of oil and improve the transporting efficiency of oil. Additionally, with its good ability of degrading oil, it can be developed for the treatment and clearing of the material such as the oil contaminated water, etc., so it can be useful in protection of the environment. This strain also has the ability of decreasing the surface tension of substances, which can be applied to the industry of biosurfactants preparation.

The invention provides a high-temperature degrading bacteria which is a geobacillus (Geobacillus) strain HN-5 and has a collection number of CGMCC (China General Microbiological Culture Collection Center) NO.5852. The bacteria can simultaneously degrade three high polymer organic matters such as cellulose, protein and starch under the aerobic conditions of about 70 DEG C, and microbial resources and technical support are provided for high-temperature aerobic composting and rapid rot.

The invention relates to a novel microbe, and in particular relates to a thermophilic bacillus and an application thereof. The strain was conserved on Oct 27, 2011, the conservation number is CCTCCM2011366, and the conservation authority is the China Center for Type Culture Collection. The strain belongs to Geobacillus category, and can be reproduced at the temperature of 80 DEG C above. According to the invention, organic matters can be efficiently and rapidly decomposed into carbon dioxide and water harmless to the environment. The thermophilic bacillus has great potential in the application of garbage treatment and the like.

The invention relates to a denitrifying bacterium which can be used for effectively utilizing an inorganic nitrogen source and inhibiting growth and reproduction of sulfate reducing bacteria (SRB) and a fermenting production method of the denitrifying bacterium, belonging to the technical field of microbial fermentation engineering. The high-temperature denitrifying bacterium provided by the invention is preserved in CGMCC, the Latin name of the high-temperature denitrifying bacterium is Geobacillus pallidas, the preservation number is CGMCC NO 7270 and the preservation date is 5th, March, 2013. The high-temperature denitrifying bacterium provided by the invention can be used for effectively utilizing the nitrogen source and effectively inhibiting growth and reproduction of SRB under a high temperature condition of 45-90 DEG C. The denitrifying bacterium is simple in fermentation process and can be successfully fermented, and the culture medium is low in cost and short in culture period. The produced denitrifying bacterium is high in viable organism content and high in germ rate. The content of unit germ cells reaches 2*10<8>cfu / mL and the content of germs reaches over 90%.

The invention relates to the technical field of environmental microorganisms, in particular to a perishable organic solid waste biological drying treatment strain and application thereof. The perishable organic solid waste biological drying treatment strain is identified as geobacillus thermophilus, named as geobacillus thermophilus FWG-JYJ1, and has an accession number is CCTCC NO: M 2020974. The geobacillus thermophilus FWGK-JYJ1 is resistant to high temperature and high in self-heat-production capacity, has the capacity of rapidly and efficiently releasing moisture in perishable organic solid waste, can be applied to the biological drying treatment process of perishable organic solid waste such as food waste, kitchen waste, fruit and vegetable waste, vegetable residues, livestock and poultry breeding excrement and municipal sludge, and achieves the effects of rapid drying and stabilization of the perishable organic solid waste.

The invention discloses a microbial compound inoculant for improving the yield and quality of codonopsis pilosula and a preparation method and application thereof, belongs to the field of microbial agricultural application, and particularly relates to the field of microbial compound inoculants. The defects that an existing microbial inoculant cannot well promote the growth of codonopsis pilosula and enhance stress resistance under normal growth conditions are overcome. The microbial compound agent is prepared from pseudomonas fluorescens with the strain preservation number of CGMCC NO: 20094, geobacillus gemini with the strain preservation number of CGMCC NO: 20095 and a cell resuscitation promoting factor Rpf protein. The microbial compound agent can remarkably promote the growth and metabolism capacity of codonopsis pilosula, the content of soluble sugar and chlorophyll in codonopsis pilosula is remarkably increased under the synergistic effect, the content of the soluble sugar and chlorophyll in codonopsis pilosula is remarkably increased, the fresh weight and the seedling height of the codonopsis pilosula seedlings are increased, particularly the root activity of the codonopsis pilosula and the ability of synthesizing SOD and PPO are improved, the growth condition of the codonopsis pilosula is obviously improved, and the yield and the quality of the codonopsis pilosula are improved.

A biologically pure culture of Geobacillus Strain T1 bacteria isolated from Palm Oil Mill Effluent capable of producing thermostable lipase T1 gene. The production of thermostable lipase T1 gene from a novel Geobacillus bacterium having a designated as strain T1, was aerobic, gram positive and endospore-forming, rod-shaped. The G+C content of the genomic DNA was 52.6%. On the basic of physiological data, phenotypic traits and molecular analysis, strain T1 represents a novel species within the genus Geobacillus, The thermostable T1 lipase gene was subcloned into the pGEX-4T1 vector with and without signal peptide in prokaryotic system. The 28 amino acid residues signal peptide alter the conformation of GST moiety and preventing it from bind to affinity glutathione Sepharose column. By expression and simplification of the purification through single step affinity chromatography shows a specific activity of 292.929 U / mg and 72.55% of fusion lipase was recovered from crude cell lysate. T1 mature lipase was successfully purified with a final recovery of 51.49% and specificity activity of 959.033 U / mg.

The invention provides a bacillus fermentation medium for efficiently producing short branched fatty acid by waste cakes and bean dreg resources and a fermentation process. Efficient production of theshort branched fatty acid is achieved by matching specific medium compositions with culture conditions. A fermentation medium formula comprises, 10-100g / L of corn starch, 20-100g / L of waste organic nitrogen sources, 1-10g / L of ammonia chloride, 3-15g / L of calcium carbonate, 1-10g / L of dipotassium phosphate, 1-10g / L of magnesium sulfate, 0.005-0.10g / L of manganese sulfate and pH (potential of hydrogen) is 6-8. Fermentation culture conditions include that culture temperature is 25-40 DEG C, the rotating speed of a shaking table is 180-240r / min, and fermentation time is 30-60 hours. According tothe fermentation medium, low-cost soybean residue, rape seed cakes and cottonseed cakes serve as fermentation raw materials. Under the process conditions, the yield of the short branched fatty acid is increased by fermentation verification of geobacillus, bacillus subtilis and bacillus amyloliquefaciens. The process has the advantages that the process is simple in assembly, environmentally friendly, economical, high in yield, stable and wide in applicable range. The fermentation medium has a certain application prospect.

The invention discloses a geobacillus strain (Geobacillus sp. YM6) with a collection number of CGMCC NO.12089 at the China General Microbiological Culture Collection center. By using leather meal as a unique carbon-nitrogen source, the strain can be connected into a fermentation culture medium with complete leather, and the leather and pinnule are mostly degraded after 48h of fermentation culture, so that the strain has remarkable leather keratin degrading effect. Optimal acting temperature of keratinase generated by the strain is 65-70 DEG C, the keratinase is high-temperature neutral keratinase, optimal pH is 7.0, and the grain can be applied in the related production field of leather degradation.

The invention belongs to the field of microorganisms, and discloses Geobacillus thermodenitrificans obtained by artificial mutagenesis and application thereof in cellulose degradation. The applicant separates a strain of Geobacillus thermoslucanase with the highest cellulase activity from a sample of pig carcass and straw compost in a high-temperature period, and obtains a strain of Geobacillus thermodenitrificans with high cellulase yield after high-temperature domestication and ultraviolet mutagenesis. The preservation number of the strain is CCTCC NO: M2020324. The Geobacillus thermodenitrificans BGSC95A1 with cellulase activity is reported for the first time. A plurality of carbon sources can be utilized, the Geobacillus thermodenitrificans has a degradation effect on a plurality of celluloses, and the cellulase concentration in fermentation liquor produced by fermentation is high.

The invention discloses a composite microbial agent for biological drying of kitchen waste, which comprises bacillus licheniformis, high-temperature alkane geobacillus and bacillus methylotrophicus, the viable count of the bacillus licheniformis is 30-40%, the viable count of the high-temperature alkane bacillus is 30-50%, and the viable count of the bacillus methylotrophicus is 20-40%; the bacillus licheniformis VH11 is preserved in the China General Microbiological Culture Collection Center on November 26, 2020, and the preservation number of the bacillus licheniformis VH11 is CGMCC (China General Microbiological Culture Collection Center) No. 21231. The microbial composite inoculant can effectively solve various problems in the reduction, stabilization, harmlessness and resource utilization process of the kitchen waste.

To provide a mixture, a dissolving solution and a pharmaceutical agent, which contain a thermophilic microorganism to make it possible to regulate a mucous membrane immune system gene cluster and metabolism-related gene clusters of the intestines and the liver. Each of them is prepared by fermentation of an organic material containing a thermophilic microorganism at a temperature of 50° C. or more and 90° C. or less. By being administered to the animal, it regulates at least one of a mucous membrane immune system gene cluster, a metabolism-related gene cluster in the intestines, and a metabolism-related gene cluster in the liver of the animal. The microorganism includes at least one species of the genus Bacillus, Oceanobacillus, Paenibacillus, Anoxybacillus, Lysinibacillus, Methanopyrus, Geogemma, Pyrolobus, Pyrodictium, Hyperthermus, Pyrococcus, Pyrobaculum, Thermococcus, Aeropyrum, Aquifex, Thermotoga, Thermodesulfobacterium, Thermus, Geobacillus, and Thermomyces.

The invention belongs to the technical field of environmental microbiology and provides a novel strain with the Latin name of Geobacillus thermoleovorans collected in China Center for Type Culture Collection (CCTCC) with a collection number of CCTCC M 20191066. Meanwhile, the invention also provides a liquid bacterial agent and a solid bacterial agent prepared from the strain. The invention further provides application of the strain in kitchen waste treatment. The strain can efficiently and rapidly degrade various organic matters in the kitchen waste under a high temperature condition and canbe used for rapid reduction treatment of the kitchen waste to realize rapid biological reduction and stabilization of the kitchen waste. The strain has remarkable aerobic biological treatment effectson the kitchen waste, can significantly reduce the treatment cost and has higher application value in the kitchen waste treatment field.

The invention relates to the technical field of sewage treatment, in particular to a compound microbial culture for degrading benzene, methylbenzene and xylene waste gas as well as a preservation method and an activation method of the compound microbial culture. The compound microbial culture comprises a bacillus, a pseudomonas, a streptococcus, a rhodococcus and a brevibacterium. The preservationmethod of the culture comprises the following steps: A1, inoculating 9-11% of inoculum dose of the compound microbial culture for degrading the benzene, methylbenzene and xylene waste gas into an aseptic enrichment medium for shake culturing to form a bacterium solution, and A2, uniformly mixing and storing the bacterium solution, a protective agent and an active stabilizing agent at -78-82 DEG C. The compound microbial culture can efficiently degrade the benzene, methylbenzene and xylene waste gas; the preservation method of the compound microbial culture is simple in technology, easy to control and low in cost; the activated compound microbial culture after preservation can continuously keep good benzene, methylbenzene and xylene degradation capability; the highly degrading benzene series degradation microbial culture is provided for quick practical engineering applications of biotreatment of the benzene series waste gas.

A method for in-situ microbial oxygen generation in an underground hydrocarbon containing formation comprises: injecting into the formation an oxygen generating composition comprising thermophilic chlorate reducing micro-organisms, such as bacteria of the genus Archaeoglobus, Geobacillus and / or Thermus, which multiply at a temperature of at least 60° C.; and inducing the multiplied micro-organisms to convert the hydrocarbons and / or other pore fluid components in-situ into transportable or disposable products

The invention provides an engineering bacterium of Geobacillusthermoglucosidasius and a construction method and application of the engineering bacterium. The construction method includes: through genetic engineering of Geobacillusthermoglucosidasius, introducing ribC (G199D) point mutation, sequentially knocking out purR, purA, ccpN and ldh genes, and then introducing an expression vector carryinga riboflavin synthesis gene cluster into the transformed engineering bacteria to obtain a series of high-temperature riboflavin-producing geobacillus engineering bacteria. The results show that yieldof riboflavin after fermentation of geobacillus after genetic engineering for 24 h is 520 mg / L; and through mixed fermentation with glucose and xylose for the strain, the yield can reach more than 1g / L. The engineering bacterium has advantages of high growth temperature and short generation time, can utilize an inexpensive carbon source, and can be used for large-scale production of riboflavin at low cost.

The invention discloses a method for improving biofilm culturing efficiency of a biological filter for treating high-nitrate wastewater, and belongs to the technical field of wastewater treatment. Themethod comprises the following operation steps of: 1, carrying out amplification culture and high-nitrate wastewater adaptability culture on geobacterium; 2, synchronously adding the cultured geobacillus and low-concentration activated sludge into a biological filter according to an equal mass ratio, and carrying out biofilm culturing; and 3, maintaining continuous water inflow of the biologicalfilter until biofilm culturing is completed. According to the invention, the abundance and bacterial extracellular polymeric substances of the geobacillus are increased by carrying out high-nitrate adaptability culture on the geobacillus and adding the cultured geobacillus into the reactor, and the growth speed, microbial activity and viscosity of microorganisms are improved, so that the biofilm culturing efficiency of the biofilter for treating the high-nitrate wastewater is improved; and the formed biological film is firm and high in impact load resistance, and has an excellent wastewater treatment effect.

The invention provides a method for in-situ fluorescent labeling of geobacillus. The method includes transferring a PpFbFP fluorescent protein gene into geobacillus. The PpFbFP fluorescent protein label is not influenced by oxygen, can make the labeled geobacillus strain fluoresce under anaerobic conditions, and has a long half-life, and can realize long-term real-time observation of movement trajectory, film forming process and group behavior of the geobacillus.