77 results about "Geobacillus" patented technology

The invention discloses a compound bacterium agent and a method for accelerating fast maturity by inoculating compound bacterium agent to compost. The method comprises the steps of: performing enlargement cultivation respectively on high-temperature microbial strains such as Geobacillus, Bacillus licheniformis, two trichoderma, actinomyces and the like which are screened from the matured compost, and combining the strains in certain proportion into the compound bacterium agent. The compound bacterium agent is inoculated to the compost in certain proportion, the compost is moved into a fermentation tank for aerated aerobic fermentation, and the compost maturity is completed. Compared with the control compost, the inoculated compost of the scheme of the invention reaches the high temperature period one week earlier than the control compost, and the high temperature period lasts for 14 days so as to be beneficial to the decomposition and humification of organic matters of the compost; and the inoculated compost is thoroughly decomposed about twenty days later. Through test, most of the weed seeds, ascarid eggs and colibacillus in the compost are dead, the humic acid content is 25 g/kg higher than that of the control compost, the humification coefficient is more than 1.9, the decomposed finished product is black brown and odourless, and has excellent stability, harmless and minimized quality, apparently environmental and economic benefits.

The present invention relates to thermostable proteinases from thermophilic Bacillus species and their uses in the preparation of nucleic acid samples. The enzymes of the invention are stable and active at 65-80 ° C., but are readily autolysed or denatured above 90° C.

The invention discloses a composite microbe oil displacement agent and the use thereof. The composite microbe oil displacement agent comprises pseudomonas fermentation anaculture and geobacillus fermentation anaculture, and the volume ratio of the pseudomonas fermentation anaculture to the geobacillus fermentation anaculture ranges from 1:10 to 10:1. The composite microbe oil displacement agent is non-toxic and harmless, is easy to degrade, and is an environmental-friendly biological agent; the manufacturing cost is low, and the composite microbe oil displacement agent is easy to manufacture and apply on a large scale; the using temperature range is wide (from 10 to 100 DEG C), and high-viscosity crude oil emulsion can be processed through emulsion breaking effectively; deposits cannot be generated after the composite microbe oil displacement agent is mixed with high hardness groundwater, and the composite microbe oil displacement agent is suitable for oil displacement, plugging removal and emulsion breaking of a low permeability oilfield and an ultra-low permeability oilfield; and the composite microbe oil displacement agent achieves high efficiency of oil displacement and emulsion breaking.

Tools and methods for detecting the presence bacteria, yeast and mold in a sample obtained from a food sample are provided. The methods employ a polymerase chain reaction and primer and probe sets that are based on the 16S rRNA and squalene-hopene cyclase genes of Alicyclobacillus and Geobacillus and the 18S rDNA gene of mold and yeast. The present invention also relates to primer and probe sets. Each primer and probe set comprises a forward primer and a reverse primer, both of which are from 15 to 35 nucleotides in length and a probe.

The invention provides a phenylalanine dehydrogenase (PDH) mutant derived from geobacillus, which is high in enzyme activity and thermal stability compared to wild PDH. Furthermore, the invention provides a method for catalytic synthesis of a saxagliptin chiral intermediate, namely (S)-N-t-butyloxycarbonyl-3-hydroxy-1-adamantyl-D-glycine (Boc-HAG) through the PDH mutant. According to the method provided by the invention, Boc-HAG can be directly prepared through a reaction in two steps, and e.e. (enantiomeric excess) value exceeds 99.9%; generation of side products can be reduced, yield of 95% can be achieved within 12 hours, catalysis time is greatly shortened, energy consumption is reduced and a post-treatment process is simplified.

The invention relates to a sulfadimidine degrading bacterium S-07 degraded by microorganisms and a screening method thereof. The sulfadimidine degrading bacterium S-07 is verified as Geobacillus, and the Genbank registration number of the bacterium 16S rDNA is KU588289. The sulfadimidine degrading bacterium is preserved in the China General Microbiological Culture Collection Center on April 21st, 2016, and the preservation number of the sulfadimidine degrading bacterium is CGMCC No. 12382. The sulfadimidine degrading bacterium S-07 well grows within the temperature range of 50-75 DEG C, has quite strong tolerance to sulfadimidine, and can efficiently degrade sulfadimidine in a liquid phase and a solid phase. The bacterium can be used for degrading and removing sulfadimidine residues in livestock excrement or sludge high-temperature compost, the removal rate is larger than 95%, and the sulfadimidine degrading bacterium S-07 has good industrial application prospects and environment benefits.

The invention provides a strain of Geobacillus thermodenitrificans as well as the screening method and the uses thereof. The strain was deposited as the number CGMCC-1228 in the China General Microbiological Culture Collection Center of the China Committee of Culture Collection for Microorganisms. The strain was obtained by primary screening, re-screening, inoculation, domestication and propagation using the bacteria in the aqueous samples from the strata of oil field as the original screening strain. The strain screened in the invention belongs to the genus Geobacillus, which can tolerate a high temperature, and has good thermostability, which can be applied to the industrial production which need the condition of thermostable enzymes, such as fermentation, etc. With its ability of growing well in the oil-reservoir environment, degrading alkanes, decreasing viscosity and increasing fluidity of crude oil, the strain is able to remarkably enhance the yield of oil and improve the transporting efficiency of oil. Additionally, with its good ability of degrading oil, it can be developed for the treatment and clearing of the material such as the oil contaminated water, etc., so it can be useful in protection of the environment. This strain also has the ability of decreasing the surface tension of substances, which can be applied to the industry of biosurfactants preparation.

The invention relates to a denitrifying bacterium which can be used for effectively utilizing an inorganic nitrogen source and inhibiting growth and reproduction of sulfate reducing bacteria (SRB) and a fermenting production method of the denitrifying bacterium, belonging to the technical field of microbial fermentation engineering. The high-temperature denitrifying bacterium provided by the invention is preserved in CGMCC, the Latin name of the high-temperature denitrifying bacterium is Geobacillus pallidas, the preservation number is CGMCC NO 7270 and the preservation date is 5th, March, 2013. The high-temperature denitrifying bacterium provided by the invention can be used for effectively utilizing the nitrogen source and effectively inhibiting growth and reproduction of SRB under a high temperature condition of 45-90 DEG C. The denitrifying bacterium is simple in fermentation process and can be successfully fermented, and the culture medium is low in cost and short in culture period. The produced denitrifying bacterium is high in viable organism content and high in germ rate. The content of unit germ cells reaches 2*10<8>cfu/mL and the content of germs reaches over 90%.

The invention discloses a microbial compound inoculant for improving the yield and quality of codonopsis pilosula and a preparation method and application thereof, belongs to the field of microbial agricultural application, and particularly relates to the field of microbial compound inoculants. The defects that an existing microbial inoculant cannot well promote the growth of codonopsis pilosula and enhance stress resistance under normal growth conditions are overcome. The microbial compound agent is prepared from pseudomonas fluorescens with the strain preservation number of CGMCC NO: 20094, geobacillus gemini with the strain preservation number of CGMCC NO: 20095 and a cell resuscitation promoting factor Rpf protein. The microbial compound agent can remarkably promote the growth and metabolism capacity of codonopsis pilosula, the content of soluble sugar and chlorophyll in codonopsis pilosula is remarkably increased under the synergistic effect, the content of the soluble sugar and chlorophyll in codonopsis pilosula is remarkably increased, the fresh weight and the seedling height of the codonopsis pilosula seedlings are increased, particularly the root activity of the codonopsis pilosula and the ability of synthesizing SOD and PPO are improved, the growth condition of the codonopsis pilosula is obviously improved, and the yield and the quality of the codonopsis pilosula are improved.

The invention discloses a geobacillus strain (Geobacillus sp. YM6) with a collection number of CGMCC NO.12089 at the China General Microbiological Culture Collection center. By using leather meal as a unique carbon-nitrogen source, the strain can be connected into a fermentation culture medium with complete leather, and the leather and pinnule are mostly degraded after 48h of fermentation culture, so that the strain has remarkable leather keratin degrading effect. Optimal acting temperature of keratinase generated by the strain is 65-70 DEG C, the keratinase is high-temperature neutral keratinase, optimal pH is 7.0, and the grain can be applied in the related production field of leather degradation.

The invention belongs to the field of microorganisms, and discloses Geobacillus thermodenitrificans obtained by artificial mutagenesis and application thereof in cellulose degradation. The applicant separates a strain of Geobacillus thermoslucanase with the highest cellulase activity from a sample of pig carcass and straw compost in a high-temperature period, and obtains a strain of Geobacillus thermodenitrificans with high cellulase yield after high-temperature domestication and ultraviolet mutagenesis. The preservation number of the strain is CCTCC NO: M2020324. The Geobacillus thermodenitrificans BGSC95A1 with cellulase activity is reported for the first time. A plurality of carbon sources can be utilized, the Geobacillus thermodenitrificans has a degradation effect on a plurality of celluloses, and the cellulase concentration in fermentation liquor produced by fermentation is high.

The invention discloses a composite microbial agent for biological drying of kitchen waste, which comprises bacillus licheniformis, high-temperature alkane geobacillus and bacillus methylotrophicus, the viable count of the bacillus licheniformis is 30-40%, the viable count of the high-temperature alkane bacillus is 30-50%, and the viable count of the bacillus methylotrophicus is 20-40%; the bacillus licheniformis VH11 is preserved in the China General Microbiological Culture Collection Center on November 26, 2020, and the preservation number of the bacillus licheniformis VH11 is CGMCC (China General Microbiological Culture Collection Center) No. 21231. The microbial composite inoculant can effectively solve various problems in the reduction, stabilization, harmlessness and resource utilization process of the kitchen waste.

To provide a mixture, a dissolving solution and a pharmaceutical agent, which contain a thermophilic microorganism to make it possible to regulate a mucous membrane immune system gene cluster and metabolism-related gene clusters of the intestines and the liver. Each of them is prepared by fermentation of an organic material containing a thermophilic microorganism at a temperature of 50° C. or more and 90° C. or less. By being administered to the animal, it regulates at least one of a mucous membrane immune system gene cluster, a metabolism-related gene cluster in the intestines, and a metabolism-related gene cluster in the liver of the animal. The microorganism includes at least one species of the genus Bacillus, Oceanobacillus, Paenibacillus, Anoxybacillus, Lysinibacillus, Methanopyrus, Geogemma, Pyrolobus, Pyrodictium, Hyperthermus, Pyrococcus, Pyrobaculum, Thermococcus, Aeropyrum, Aquifex, Thermotoga, Thermodesulfobacterium, Thermus, Geobacillus, and Thermomyces.

The invention belongs to the technical field of environmental microbiology and provides a novel strain with the Latin name of Geobacillus thermoleovorans collected in China Center for Type Culture Collection (CCTCC) with a collection number of CCTCC M 20191066. Meanwhile, the invention also provides a liquid bacterial agent and a solid bacterial agent prepared from the strain. The invention further provides application of the strain in kitchen waste treatment. The strain can efficiently and rapidly degrade various organic matters in the kitchen waste under a high temperature condition and canbe used for rapid reduction treatment of the kitchen waste to realize rapid biological reduction and stabilization of the kitchen waste. The strain has remarkable aerobic biological treatment effectson the kitchen waste, can significantly reduce the treatment cost and has higher application value in the kitchen waste treatment field.

A method for in-situ microbial oxygen generation in an underground hydrocarbon containing formation comprises: injecting into the formation an oxygen generating composition comprising thermophilic chlorate reducing micro-organisms, such as bacteria of the genus Archaeoglobus, Geobacillus and/or Thermus, which multiply at a temperature of at least 60° C.; and inducing the multiplied micro-organisms to convert the hydrocarbons and/or other pore fluid components in-situ into transportable or disposable products

The invention provides an engineering bacterium of Geobacillusthermoglucosidasius and a construction method and application of the engineering bacterium. The construction method includes: through genetic engineering of Geobacillusthermoglucosidasius, introducing ribC (G199D) point mutation, sequentially knocking out purR, purA, ccpN and ldh genes, and then introducing an expression vector carryinga riboflavin synthesis gene cluster into the transformed engineering bacteria to obtain a series of high-temperature riboflavin-producing geobacillus engineering bacteria. The results show that yieldof riboflavin after fermentation of geobacillus after genetic engineering for 24 h is 520 mg/L; and through mixed fermentation with glucose and xylose for the strain, the yield can reach more than 1g/L. The engineering bacterium has advantages of high growth temperature and short generation time, can utilize an inexpensive carbon source, and can be used for large-scale production of riboflavin at low cost.

The invention provides a method for in-situ fluorescent labeling of geobacillus. The method includes transferring a PpFbFP fluorescent protein gene into geobacillus. The PpFbFP fluorescent protein label is not influenced by oxygen, can make the labeled geobacillus strain fluoresce under anaerobic conditions, and has a long half-life, and can realize long-term real-time observation of movement trajectory, film forming process and group behavior of the geobacillus.

The invention discloses geobacillus thermodenitrificans engineering bacteria capable of efficiently degrading nitroalkanes. The geobacillus thermodenitrificans engineering bacteria capable of efficiently degrading the nitroalkanes are obtained by using an NOEs (nitroalkane-oxidizing enzymes) gene that overexpresses a nitroalkane degradation pathway. The modified engineering bacteria are named NG-S1 and NG-S2. The nucleotide sequence of the NOEs gene is represented by SEQ ID NO. 1-2. Compared with wild-type geobacillus thermodenitrificans, the obtained engineering bacteria have the advantages that the degradation efficiency is obviously improved, and the degradation rates of the NG-S1 (under anaerobic conditions) and the NG-S2 (under aerobic conditions) are increased by 2 times and 2.8 times respectively. The two high-temperature-resistant strains capable of efficiently degrading toxic pollutants respectively in an aerobic environment and in an anaerobic environment are provided, and have great significance and application value in treatment of environmental pollution; new methods and examples are provided for modification of thermophilic engineering bacteria for biodegradation.