3 results about "Geobacillus" patented technology

The present application relates to the technical field of analytical detection, and in particular to a detection method of Geobacillus thermophilus in raw milk based on real-time fluorescent quantitative polymerase chain reaction. The detection method of the present application is based on nucleic acid specific detection mechanism, which replaces the phenotype culture mechanism, realizes the rapid and accurate quantification of the target bacteria, and replaces the time-consuming and inaccurate colony counting. Through the design of specific primers and probes, the species level specificity identification is realized, and the problem that the traditional method cannot distinguish the specific contaminated bacteria is solved. Through the optimization of the pretreatment method, the sensitivity of the detection method is greatly improved. The detection method of the present application shortens the detection time, realizes the leap from "days" to "hours", greatly improves the detection efficiency, and has a wide application prospect.

This invention provides a *Bacillus hygroscopicus* strain and its application, relating to the field of microbial fermentation technology. The strain is named *Bacillus hygroscopicus* (…). Bacillus altitudinis Bacillus subtilis (W09), deposited at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, China, Institute of Microbiology, Chinese Academy of Sciences, on December 26, 2025, with accession number CGMCC No. 37218. This invention provides Bacillus subtilis (W09). Bacillus altitudinis W09 can be used to increase the content of schisandrol A in the fermentation products of Schisandra chinensis vine stems.