27 results about "Geobacillus" patented technology

The invention discloses an extreme thermophilic charcoal complex microbial inoculant and application thereof in aerobic composting of pig manure, and belongs to the technical field of microorganisms. The invention firstly discloses a strain of thermophilic thermophilic bacteria N1209, and secondly discloses an extreme thermophilic biochar complex microbial inoculant, which comprises geobacillus thermoglucosidase BGSC 95A1, bacillus licheniformis ZR-1, the thermophilic thermophilic thermophilic bacteria N1209 and geobacillus stearothermophilus L5. The extremely thermophilic biochar complex microbial inoculant has the characteristic of high temperature resistance, compared with a single microbial inoculant, produced amylase, protease and lipase are higher in activity and show synergistic advantages, decomposition of pig manure can be remarkably promoted, the extremely thermophilic biochar complex microbial inoculant has advantages in the aspects of nitrogen element retention and CH4 and N2O emission reduction in the pig manure composting process, and the extremely thermophilic biochar complex microbial inoculant can be applied to the field of pig manure composting. And a new method is provided for treatment of livestock breeding wastes.

A 16SrDNA sequence of the ruminant rumen-source bacillus ginseng geobacillus is shown as SEQ ID NO.1. A strain is collected from a ruminant rumen and inoculated to a culture medium with straw as a substrate for anaerobic culture and passage enrichment culture to obtain a dominant strain utilizing the straw, screening culture of cellulosic bacteria is performed, single colony selection and purification are performed, and the ruminant rumen-source bacillus ginseng geobacillus is obtained. The strain with the cellulose monodegradation capability is obtained. The bacillus panacis disclosed by the invention is high in adaptability, can effectively ferment feed in aerobic and anoxic environments, can quickly start fermentation, is high in stability in the fermentation process, improves the quality of the feed and reduces the feeding cost.

The invention belongs to the field of microbial agents, and particularly relates to a compost heat production method of a ganoderma lucidum basswood planting waste substrate, which comprises the following steps: mixing the ganoderma lucidum basswood planting waste substrate with chicken manure to obtain a fermentation raw material; paving the fermentation raw materials in a fermentation container layer by layer; a complex microbial inoculant is uniformly sprayed on the surface of each layer of fermentation raw material; the complex microbial inoculant is prepared from the following strains in percentage by weight: 10 to 25 percent of bacillus cereus, 5 to 10 percent of bacillus smith, 5 to 10 percent of geobacillus thermodenitrificans, 20 to 40 percent of bacillus licheniformis, 5 to 24 percent of ureibacillus thermophilus and the balance of actinomyces thermophilus; the oxygen content is controlled to be 5-15%; the 50-60 DEG C high-temperature period is maintained for 180-210 days, the heat exchange pipe located between the two layers of fermentation raw materials is connected to a heating system in the high-temperature period, and the system is suitable for agricultural circular economy and distributed energy supply scenes.

The invention provides an application of GH48 / GH57 / GH9 family genes carried by bacillus altitudinis H1 in improvement of corn straw rumen fermentation digestibility, and belongs to the technical field of degrading bacteria. According to the invention, a cellulose degradation strain H1 is separated and screened from a goat rumen content, and the preservation number of the strain is GDMCC No: 67152. The invention further discloses a preparation method of the cellulose degradation strain H1. An in-vitro fermentation experiment shows that the soluble carbohydrate content and the lignin content of the corn straw fermented by the H1 strain are remarkably reduced, the 48-hour gas production rate is remarkably reduced, and the propionic acid content is also remarkably reduced. The result shows that the H1 strain has stronger lignin decomposition capability and preferentially degrades hemicellulose and pectin. The H1 strain provided by the invention is proved to have a good corn straw degradation effect.

The present application relates to the technical field of analytical detection, and in particular to a detection method of Geobacillus thermophilus in raw milk based on real-time fluorescent quantitative polymerase chain reaction. The detection method of the present application is based on nucleic acid specific detection mechanism, which replaces the phenotype culture mechanism, realizes the rapid and accurate quantification of the target bacteria, and replaces the time-consuming and inaccurate colony counting. Through the design of specific primers and probes, the species level specificity identification is realized, and the problem that the traditional method cannot distinguish the specific contaminated bacteria is solved. Through the optimization of the pretreatment method, the sensitivity of the detection method is greatly improved. The detection method of the present application shortens the detection time, realizes the leap from "days" to "hours", greatly improves the detection efficiency, and has a wide application prospect.

The invention discloses a difunctional alcohol dehydrogenase derived from Geobacillus thermodenitrifis, the difunctional alcohol dehydrogenase takes NAD < + > as a cofactor, can specifically catalyze a continuous oxidation reaction of omega-hydroxy fatty acid, and catalyzes the omega-hydroxy fatty acid to synthesize alpha, omega-binary fatty acid through single enzyme catalysis without an aldehyde intermediate. The alcohol dehydrogenase has dual catalytic functions of alcohol oxidation and aldehyde oxidation, the oxidation process from hydroxymethylene to carboxyl can be completed in one step, the reaction steps are simplified, accumulation and separation of intermediate aldehyde are avoided, the conversion efficiency is improved, and the alcohol dehydrogenase is alpha, omega-dibasic fatty acid and is a novel alcohol dehydrogenase. The invention provides a simple and efficient biocatalysis way for biosynthesis of dodecanedioic acid, especially dodecanedioic acid, and has important industrial application value.

Provided herein is a protein from Tumebacillus sp. NITE BP-02779 having a peptidoglycan-decomposing activity. Provided herein is a protein comprising the amino acid sequence of SEQ ID NO: 2 (1-164) or the amino acid sequence of SEQ ID NO: 4 (1-493), or a protein comprising an amino acid sequence in which 1 to 10 amino acid residues of these amino acid sequences are substituted, deleted, inserted, or added, or an amino acid sequence having 90% or more identity to these amino acid sequences, and having a peptidoglycan-decomposing activity.

The invention relates to a microbial agent for removing refractory COD (Chemical Oxygen Demand) in wastewater and application of the microbial agent, and belongs to the technical field of biological purification. The invention provides a microbial agent for removing refractory COD (Chemical Oxygen Demand) in wastewater. The microbial agent is prepared from two or more of geomonas, rope warming bacteria, nitro-spirillum, thiobacillus, urea bacillus, leptospirillum, piriformospora, floating fungi, nitro-spina bacteria, thauera and hyphomicrobe. The microbial agent can remove part of refractory COD (Chemical Oxygen Demand) by utilizing the synergistic effect and co-metabolism of biocenosis, and has a great application prospect in the treatment of wastewater (such as landfill leachate and the like) containing a large amount of refractory COD.

The present invention discloses a rapid start-up process for shortcut nitrification of wastewater with high ammonia nitrogen. First, according to the characteristics of shortcut nitrification of wastewater with high ammonia nitrogen, the pH, temperature, ammonia nitrogen concentration and dissolved oxygen concentration are adjusted before water inlet to make the inlet load meet the requirements of microbial degradation. Then, strain NZ-AY1 is added, and the rapid start-up process for shortcut nitrification is carried out under the conditions of high ammonia nitrogen concentration, 30-33 °C, and pH 7.0-8.5. The strain is classified and named as Geobacillus inquilinus ([[]] Parageobacillus toebii ) NZ-AY1, which has been deposited in the China General Microbiological Culture Collection Center. In this process, the ammonia nitrogen removal efficiency can reach over 95%, the ammonia oxidation efficiency can reach 87% to 95%, and the ability to resist various shock loads is strong, making it more stable in the treatment system. At the same time, the rapid expansion culture process of the strain is also disclosed, which has the advantages of simple operation, short production cycle and low production cost, has practical application value, and broad market prospects.

The present invention relates to a method for inhibiting biogenic hydrogen sulfide in oilfields. A synergistic inhibition system combination agent containing Geobacillus strain GW1 is added into oilfield systems, wherein the strain GW1 has been deposited in China General Microbiological Culture Collection Center on Oct. 20, 2021, and has the deposit number of CGMCC No. 23631.

The application discloses a heterotrophic nitrifying bacterium D31, which is classified as Geobacillus proteiniphilus and has a strain preservation number of CGMCC NO.27788. The strain D31 can perform heterotrophic nitrification on a culture medium containing ammonia nitrogen, can grow on a simple carbon and nitrogen source culture medium and oxidize ammonia. The strain D31 can utilize simple nutrients to rapidly proliferate, is inoculated into compost after aerobic fermentation, can efficiently and rapidly oxidize ammonia nitrogen in livestock and poultry manure into nitrate nitrogen in a high-temperature period, reduces the volatilization of nitrogen elements in the form of ammonia gas in the compost, can not only reduce odor emission and protect the environment, but also can maintain nitrogen nutritional elements in the compost, improves compost quality, and has a wide application prospect. The application further provides application of the heterotrophic nitrifying bacterium D31 in livestock and poultry manure composting.

The application discloses an extremely thermophilic biochar composite microbial agent and application thereof in pig manure aerobic composting, and belongs to the technical field of microorganisms. The application discloses a strain of thermophilic thermus N1209, and further discloses an extremely thermophilic biochar composite microbial agent, which comprises thermophilic glucosidase geobacillus BGSC 95A1, bacillus licheniformis ZR-1, thermus N1209 and thermophilic adipose geobacillus L5. The extremely thermophilic biochar composite microbial agent has the characteristics of high temperature resistance, higher amylase, protease and lipase activity compared with single microbial agent, shows synergistic advantages, can significantly promote the composting of pig manure, has advantages in the aspects of nitrogen element reservation and CH4 and N2O emission reduction in the pig manure composting process, and provides a new method for the treatment of poultry breeding waste.

The application discloses an endoglucanase from Geobacillus sp. Geobacillus sp. 70PC53)as a raw enzyme, discloses three endoglucanase mutants with improved thermal stability, which are V217L, N264Y and R170P / N264Y respectively. The endoglucanase mutants provided by the application have better thermal stability than the raw enzyme, and the residual enzyme activity of the mutants can be increased by 217% compared with the raw enzyme after 95℃ treatment. The relative enzyme activity of the endoglucanase mutants provided by the application is higher than that of the raw enzyme within 65℃~95℃, and the endoglucanase mutants can maintain good catalytic performance in a higher and wider temperature range, and have a good industrial application prospect.

The invention discloses a geobacillus thermophilus strain and an application of the geobacillus thermophilus strain. The Bacillus variotii Z231 has been preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on October 21, 2025, the address of the Guangdong Microbial Culture Collection Center (GDMCC) is the 10th floor of the No.58 floor of the No.100 Courtyard, the Xianlie Middle Road, Guangzhou City, Guangdong Province, the postcode is 510070, and the preservation number is GDMCC No: 67147. The growth temperature of the Geobacillus variotii Z231 is 60 DEG C, and the Geobacillus variotii Z231 has hydrolytic activity on PET oligomer BHET and can tolerate high-concentration PET hydrolysates TPA (terephthalic acid) and EG (ethylene glycol), so that the Geobacillus variotii Z231 has good application potential in the aspects of PET plastic biodegradation and upgrading conversion.

The invention discloses a water quality purifying agent based on microalgae fermentation. The water quality purifying agent is prepared from alkaline protease, cellulase, a spirulina extract, bacillus subtilis, geobacillus, bacillus amyloliquefaciens, thermophilic bacteria, halotolerant bacteria, nano calcium carbonate, chitosan and spirulina micro powder. Microalgae, microorganisms and an enzyme preparation are adopted to form a synergistic system, the purification effect stability is improved, the efficient and long-acting water quality purification requirements in an extreme environment are met, and the water quality purifying agent has dynamic adaptability, synergistic interaction and environmental friendliness. The preparation method comprises the following steps: directional pretreatment of spirulina, construction of a dynamic flora proportion regulation and control system, culture of complex functional flora, preparation of a nano composite carrier, preparation of a bacterium-algae enzyme complexing agent and the like.

The application provides a Paenibacillus-like strain and application thereof, the Paenibacillus-like strain is preserved in Guangdong Microbial Culture Collection Center on August 4, 2025, and the preservation number is GDMCC No: 66801; the application is to prepare a preparation comprising the Paenibacillus-like strain, a preparation for preventing or inhibiting plant pathogenic fungi from invading plants; the Paenibacillus-like strain can effectively and durably inhibit the growth of plant pathogenic fungi, can be used as a broad-spectrum antibacterial agent, enhances the resistance of plants, and promotes the growth of plants.

The invention discloses a high-temperature aerobic zymophyte with a function of efficiently degrading organic matters, which is characterized in that the high-temperature aerobic zymophyte is Geobacillus bin1 and is preserved in China General Microbiological Culture Collection Center on October 13, 2025, and the preservation number is CGMCC No.36159. The invention further discloses a preparation method of the high-temperature aerobic zymophyte. The high-temperature aerobic zymophyte shows a huge application prospect and a wide application range, and covers a plurality of important fields, such as sludge treatment, straw conversion, fermentation treatment of garden wastes and the like. In the aerobic fermentation process of the organic solid waste, the strain researched by the invention has the remarkable advantages of high temperature rise speed, high highest fermentation temperature, long high-temperature maintenance period and more thorough degradation of organic matters. Due to the characteristics, the strain has high practical value in the aspects of environmental governance and resource recycling.

The invention relates to the technical field of agriculture, and discloses a microorganism-mineral based composite material as well as a preparation method and application thereof. The microorganism-mineral-based composite material comprises the following components: iron phosphate powder and dissimilatory iron reduction microorganisms, wherein the dissimilatory iron reducing microorganisms comprise at least one of shewanella, geobacter, desulfurization vibrio, clostridium butyricum, geoacidophilus and iron-rich bacteria. The microorganism-mineral-based composite material provided by the invention comprises the mineral component iron phosphate and dissimilatory iron reduction microorganisms, and the dissimilatory iron reduction microorganisms can actively reduce the iron phosphate which is originally stable in chemical property and insoluble into Fe (II) with relatively high solubility, so that the crystal structure of the iron phosphate is destroyed; the phosphate fertilizer can release free PO4 < 3-> which can be absorbed and utilized by plants, the effect of the mineral reduction dissolution process driven by microorganisms is far better than that of simple addition when minerals or microorganisms are independently used, and an efficient phosphorus activation mechanism is formed.

This invention provides a *Bacillus hygroscopicus* strain and its application, relating to the field of microbial fermentation technology. The strain is named *Bacillus hygroscopicus* (…). Bacillus altitudinis Bacillus subtilis (W09), deposited at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, China, Institute of Microbiology, Chinese Academy of Sciences, on December 26, 2025, with accession number CGMCC No. 37218. This invention provides Bacillus subtilis (W09). Bacillus altitudinis W09 can be used to increase the content of schisandrol A in the fermentation products of Schisandra chinensis vine stems.

The invention discloses a geobacillus stearothermophilus HD-6, and the preservation number of the geobacillus stearothermophilus HD-6 is CGMCC (China General Microbiological Culture Collection Center) No.28135. The geobacillus stearothermophilus HD-6 has the advantages that the preservation number is CGMCC No.28135; the geobacillus stearothermophilus HD-6 has the following beneficial effects: 1, the geobacillus stearothermophilus HD-6 has the advantages of low cost, simple process and the like when being used for degrading polyacrylamide; 2, the geobacillus stearothermophilus HD-6 has good oil reservoir adaptability, can degrade polyacrylamide under the condition that the oil reservoir temperature is higher than 60 DEG C, makes up for the defect that only sulfate reducing bacteria can degrade under the condition but hydrogen sulfide is generated, does not generate toxic AM in the polyacrylamide degradation process, reduces potential safety hazards, is safe and environment-friendly, and has wide application prospects. And secondary pollution is not easily caused.

The application discloses an endoglucanase from Geobacillus sp. Geobacillus sp. 70PC53)as a raw enzyme, and discloses three endoglucanase mutants with improved pH tolerance, namely V217L, A123S / I165V and A123G / I165L / V185S / V217C. The endoglucanase mutant provided by the application is superior to the raw enzyme in pH tolerance, and the residual enzyme activity of the mutant can be increased by 66% compared with the raw enzyme under the condition of pH 9.0; the relative enzyme activity of the endoglucanase mutant provided by the application is higher than that of the raw enzyme within pH 3.0-11.0, and the endoglucanase mutant can maintain stable and efficient catalytic performance under the condition, and has a good industrial application prospect.

The present invention discloses a leaf-sphere growth-promoting Geobacillus sp. for enhancing the resistance of rice to high temperature stress and its application. Geobacillus thermoparaffinivorans ) LspGeo, deposited with the General Microbiology Center of the China General Culture Collection Administration on February 28, 2025, with the deposit number CGMCC No. 33681. The application of the thermophilic Geobacillus sp. LspGeo in improving the heat stress tolerance and / or growth promotion of rice is of great significance for the development of superior bacterial strain resources that can enhance the heat tolerance and recovery of rice, as well as for the development of biological agents that specifically enhance the heat tolerance of rice.

The invention discloses a rhizosphere growth-promoting geobacillus for enhancing high-temperature stress resistance of rice and application of the rhizosphere growth-promoting geobacillus. The invention relates to a bacillus thermophilus LspGeo, which is preserved in China General Microbiological Culture Collection Center (CGMCC) on February 28, 2025, and the preservation number of the bacillus thermophilus LspGeo is CGMCC No.33681. The bacillus thermophilus LspGeo has the advantages that the bacillus thermophilus LspGeo is preserved in the China General Microbiological Culture Collection Center (CGMCC) on February 28, 2025; the invention also discloses the application of the bacillus thermophilus LspGeo in improving the heat stress resistance of the rice and / or promoting the growth of the rice. The invention has important significance for developing excellent strain resources capable of improving the heat resistance and the recovery capability of the rice and developing biological preparations capable of improving the heat resistance of the rice in a targeted manner.

The invention discloses a biosensor capable of detecting explosive molecules under a high-temperature condition as well as a preparation method and application of the biosensor. The host of the biosensor is geobacillus thermoglucosidase, and the microbial biosensor comprises a yqjfC55 promoter as shown in SEQ ID NO.1 and a green fluorescent protein sfGreen which is as shown in SEQ ID NO.2, can be quickly folded and is resistant to high temperature. The growth temperature of the geobacillus thermoglucosidase is 42-69 DEG C, and the optimum growth temperature is 60 DEG C. The biosensor disclosed by the invention realizes detection of explosive molecules under a high-temperature condition, and the application scene is expanded.

Described herein are methods and compositions for treating cancer and for predicting a subjects' response to combination checkpoint inhibitor therapy. Aspects of the disclosure relate to a method of treating cancer and / or reducing toxicity to a therapy in a subject comprising administering to the subject a composition comprising at least one isolated or purified population of bacteria belonging to one or more of the genera Flavonifractor, Dielma, Akkermansia, Alistipes, Bacteroides, Butyricimonas, Vampirovibrio, Tyzzerella, Parabacteroides distasonis, Fournierella, Fournierella massiliensis, Eisenbergiella tayi, Tissierellales, Hungateiclostridium thermocellum, Dorea formicigenerans, Caloramator coolhaasi, Muricomes, Geosporobacter, Prevotella paludivivens, Lactobacillus secaliphilus, Bacteroides finegoldii, Lactobacillus johnsonii, Parapedobacter composti, and Anaerotignum lactatifermentans and wherein the method further comprises treating the subject with a combination of (i) a PD-1, PDL1, or PDL2 inhibitor and (ii) a CTLA-4, B7-1, or B7-2 inhibitor.