13 results about "Geobacillus" patented technology

The invention provides an application of GH48 / GH57 / GH9 family genes carried by bacillus altitudinis H1 in improvement of corn straw rumen fermentation digestibility, and belongs to the technical field of degrading bacteria. According to the invention, a cellulose degradation strain H1 is separated and screened from a goat rumen content, and the preservation number of the strain is GDMCC No: 67152. The invention further discloses a preparation method of the cellulose degradation strain H1. An in-vitro fermentation experiment shows that the soluble carbohydrate content and the lignin content of the corn straw fermented by the H1 strain are remarkably reduced, the 48-hour gas production rate is remarkably reduced, and the propionic acid content is also remarkably reduced. The result shows that the H1 strain has stronger lignin decomposition capability and preferentially degrades hemicellulose and pectin. The H1 strain provided by the invention is proved to have a good corn straw degradation effect.

The present application relates to the technical field of analytical detection, and in particular to a detection method of Geobacillus thermophilus in raw milk based on real-time fluorescent quantitative polymerase chain reaction. The detection method of the present application is based on nucleic acid specific detection mechanism, which replaces the phenotype culture mechanism, realizes the rapid and accurate quantification of the target bacteria, and replaces the time-consuming and inaccurate colony counting. Through the design of specific primers and probes, the species level specificity identification is realized, and the problem that the traditional method cannot distinguish the specific contaminated bacteria is solved. Through the optimization of the pretreatment method, the sensitivity of the detection method is greatly improved. The detection method of the present application shortens the detection time, realizes the leap from "days" to "hours", greatly improves the detection efficiency, and has a wide application prospect.

The invention discloses a difunctional alcohol dehydrogenase derived from Geobacillus thermodenitrifis, the difunctional alcohol dehydrogenase takes NAD < + > as a cofactor, can specifically catalyze a continuous oxidation reaction of omega-hydroxy fatty acid, and catalyzes the omega-hydroxy fatty acid to synthesize alpha, omega-binary fatty acid through single enzyme catalysis without an aldehyde intermediate. The alcohol dehydrogenase has dual catalytic functions of alcohol oxidation and aldehyde oxidation, the oxidation process from hydroxymethylene to carboxyl can be completed in one step, the reaction steps are simplified, accumulation and separation of intermediate aldehyde are avoided, the conversion efficiency is improved, and the alcohol dehydrogenase is alpha, omega-dibasic fatty acid and is a novel alcohol dehydrogenase. The invention provides a simple and efficient biocatalysis way for biosynthesis of dodecanedioic acid, especially dodecanedioic acid, and has important industrial application value.

Provided herein is a protein from Tumebacillus sp. NITE BP-02779 having a peptidoglycan-decomposing activity. Provided herein is a protein comprising the amino acid sequence of SEQ ID NO: 2 (1-164) or the amino acid sequence of SEQ ID NO: 4 (1-493), or a protein comprising an amino acid sequence in which 1 to 10 amino acid residues of these amino acid sequences are substituted, deleted, inserted, or added, or an amino acid sequence having 90% or more identity to these amino acid sequences, and having a peptidoglycan-decomposing activity.

The application discloses a heterotrophic nitrifying bacterium D31, which is classified as Geobacillus proteiniphilus and has a strain preservation number of CGMCC NO.27788. The strain D31 can perform heterotrophic nitrification on a culture medium containing ammonia nitrogen, can grow on a simple carbon and nitrogen source culture medium and oxidize ammonia. The strain D31 can utilize simple nutrients to rapidly proliferate, is inoculated into compost after aerobic fermentation, can efficiently and rapidly oxidize ammonia nitrogen in livestock and poultry manure into nitrate nitrogen in a high-temperature period, reduces the volatilization of nitrogen elements in the form of ammonia gas in the compost, can not only reduce odor emission and protect the environment, but also can maintain nitrogen nutritional elements in the compost, improves compost quality, and has a wide application prospect. The application further provides application of the heterotrophic nitrifying bacterium D31 in livestock and poultry manure composting.

The application discloses an extremely thermophilic biochar composite microbial agent and application thereof in pig manure aerobic composting, and belongs to the technical field of microorganisms. The application discloses a strain of thermophilic thermus N1209, and further discloses an extremely thermophilic biochar composite microbial agent, which comprises thermophilic glucosidase geobacillus BGSC 95A1, bacillus licheniformis ZR-1, thermus N1209 and thermophilic adipose geobacillus L5. The extremely thermophilic biochar composite microbial agent has the characteristics of high temperature resistance, higher amylase, protease and lipase activity compared with single microbial agent, shows synergistic advantages, can significantly promote the composting of pig manure, has advantages in the aspects of nitrogen element reservation and CH4 and N2O emission reduction in the pig manure composting process, and provides a new method for the treatment of poultry breeding waste.

The application provides a Paenibacillus-like strain and application thereof, the Paenibacillus-like strain is preserved in Guangdong Microbial Culture Collection Center on August 4, 2025, and the preservation number is GDMCC No: 66801; the application is to prepare a preparation comprising the Paenibacillus-like strain, a preparation for preventing or inhibiting plant pathogenic fungi from invading plants; the Paenibacillus-like strain can effectively and durably inhibit the growth of plant pathogenic fungi, can be used as a broad-spectrum antibacterial agent, enhances the resistance of plants, and promotes the growth of plants.

The invention discloses a high-temperature aerobic zymophyte with a function of efficiently degrading organic matters, which is characterized in that the high-temperature aerobic zymophyte is Geobacillus bin1 and is preserved in China General Microbiological Culture Collection Center on October 13, 2025, and the preservation number is CGMCC No.36159. The invention further discloses a preparation method of the high-temperature aerobic zymophyte. The high-temperature aerobic zymophyte shows a huge application prospect and a wide application range, and covers a plurality of important fields, such as sludge treatment, straw conversion, fermentation treatment of garden wastes and the like. In the aerobic fermentation process of the organic solid waste, the strain researched by the invention has the remarkable advantages of high temperature rise speed, high highest fermentation temperature, long high-temperature maintenance period and more thorough degradation of organic matters. Due to the characteristics, the strain has high practical value in the aspects of environmental governance and resource recycling.

The invention relates to the technical field of agriculture, and discloses a microorganism-mineral based composite material as well as a preparation method and application thereof. The microorganism-mineral-based composite material comprises the following components: iron phosphate powder and dissimilatory iron reduction microorganisms, wherein the dissimilatory iron reducing microorganisms comprise at least one of shewanella, geobacter, desulfurization vibrio, clostridium butyricum, geoacidophilus and iron-rich bacteria. The microorganism-mineral-based composite material provided by the invention comprises the mineral component iron phosphate and dissimilatory iron reduction microorganisms, and the dissimilatory iron reduction microorganisms can actively reduce the iron phosphate which is originally stable in chemical property and insoluble into Fe (II) with relatively high solubility, so that the crystal structure of the iron phosphate is destroyed; the phosphate fertilizer can release free PO4 < 3-> which can be absorbed and utilized by plants, the effect of the mineral reduction dissolution process driven by microorganisms is far better than that of simple addition when minerals or microorganisms are independently used, and an efficient phosphorus activation mechanism is formed.

This invention provides a *Bacillus hygroscopicus* strain and its application, relating to the field of microbial fermentation technology. The strain is named *Bacillus hygroscopicus* (…). Bacillus altitudinis Bacillus subtilis (W09), deposited at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, China, Institute of Microbiology, Chinese Academy of Sciences, on December 26, 2025, with accession number CGMCC No. 37218. This invention provides Bacillus subtilis (W09). Bacillus altitudinis W09 can be used to increase the content of schisandrol A in the fermentation products of Schisandra chinensis vine stems.

Described herein are methods and compositions for treating cancer and for predicting a subjects' response to combination checkpoint inhibitor therapy. Aspects of the disclosure relate to a method of treating cancer and / or reducing toxicity to a therapy in a subject comprising administering to the subject a composition comprising at least one isolated or purified population of bacteria belonging to one or more of the genera Flavonifractor, Dielma, Akkermansia, Alistipes, Bacteroides, Butyricimonas, Vampirovibrio, Tyzzerella, Parabacteroides distasonis, Fournierella, Fournierella massiliensis, Eisenbergiella tayi, Tissierellales, Hungateiclostridium thermocellum, Dorea formicigenerans, Caloramator coolhaasi, Muricomes, Geosporobacter, Prevotella paludivivens, Lactobacillus secaliphilus, Bacteroides finegoldii, Lactobacillus johnsonii, Parapedobacter composti, and Anaerotignum lactatifermentans and wherein the method further comprises treating the subject with a combination of (i) a PD-1, PDL1, or PDL2 inhibitor and (ii) a CTLA-4, B7-1, or B7-2 inhibitor.