11 results about "Brevibacterium" patented technology

The present application relates to the field of microbial technology, and specifically discloses a strain of Brachybacterium paraconglomeratum 2-1 and its application in industrial wastewater treatment; the Brachybacterium paraconglomeratum 2-1 is preserved in the China General Microbiological Culture Collection Center on May 29, 2025, with a preservation number of CGMCC No.34719 and a classification name of Brachybacterium paraconglomeratum; the strain 2-1 can tolerate the high toxicity environment of residual ammonia water in a coking plant, has a good effect in the microbial treatment of industrial wastewater containing phenol, can grow by taking phenol as the only carbon source, realizes the removal of phenol in wastewater under aerobic conditions, and has great application potential in the biological treatment of industrial wastewater.

The present application relates to the field of microorganisms and biotechnology, and particularly relates to a Brevibacillus parafacis and application thereof in degrading polyphenols in tobacco leaves. The Brevibacillus parafacis is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 63066, and the preservation date is December 20, 2022. The strain can effectively degrade the content of polyphenols in cigar tobacco leaves, especially chlorogenic acid.

The invention discloses a microbial agent for improving disease resistance of tea trees and a preparation method of the microbial agent, and belongs to the field of agricultural disease prevention and control. According to the invention, the optimal strain compounding condition is obtained by screening the strain compounding condition, and experiments prove that the fungicide prepared when the brevibacterium Hainan powder ACCC 60371 and the streptomyces griseus var rust CICC 23624 are compounded according to the mass ratio of 2: 5 can enable the prevention and treatment effects of tea leaf blight and zonate spot to reach 88.58% and 84.41% respectively, so that the fungicide can be used for preventing and treating the tea leaf blight and zonate spot respectively. And the polyphenol content is obviously superior to the effect of preventing and treating the two tea tree diseases by single bacteria. The invention provides a strain source and a method for improving disease resistance and tea quality.

The application discloses a process for removing pollutants in factory breeding tail water of penaeus vannamei, and belongs to the technical field of breeding tail water treatment. The process comprises the following steps: (1) filtering breeding wastewater to remove solid matters; (2) adding composite bacteria to the filtered breeding wastewater for aeration treatment to obtain aeration wastewater, wherein the composite bacteria comprise, by weight, 1-2 parts of rhodococcus, 2-3 parts of bacillus subtilis, 3-4 parts of halococcus and 0.8-1.5 parts of photosynthetic bacteria; (3) adding a flocculating agent to the aeration wastewater, treating the aeration wastewater at 45-50 DEG C for 0.5-1 h, and filtering to obtain a first filtrate; (4) adding modified activated carbon to the first filtrate, treating the first filtrate at 45-50 DEG C for 1-2 h, filtering to obtain a second filtrate; and (5) flowing and mixing the second filtrate through a macroporous adsorption resin to obtain a third filtrate, and sterilizing the third filtrate. The application realizes efficient removal of pollutants in the breeding tail water of penaeus vannamei through the combination of physical, chemical and biological treatments.

The invention provides a marine sediment pumilus Thr32 strain and application thereof. The preservation number of the Thr32 strain is GDMCC No: 67315. The invention further provides a preparation method of the marine sediment pumilus Thr32 strain. The application of the marine sediment brevibacterium in prevention and treatment of growth of Orobanche cucurbitae is reported for the first time, and the types of strains for prevention and treatment of growth of Orobanche cucurbitae are enriched. According to the application of the Curtobacterium crenatum Thr32 provided by the invention in prevention and treatment of Orobanche cucurbitae, the inhibition rate of separated strain fermentation liquor on seed germination of Orobanche cucurbitae is as high as 100%, and the method has a wide application value in biological prevention and treatment of Orobanche cucurbitae.

The invention provides bacillus brevis HDCC00067, the preservation number of the bacillus brevis HDCC00067 is CGMCC (China General Microbiological Culture Collection Center) No.35553, and the capability of producing brevibacterium peptide A is obviously higher than that of the prior art. The applicant also finds that the yield of the brevibacitracin A can be obviously improved when amino acid precursor substances are added into the fermentation culture medium, especially when the concentrations of L-tryptophan, D-leucine and D-valine are controlled to be within a certain range. By controlling pH and dissolved oxygen in a tank fermentation culture medium and optimizing a feeding control strategy of a mixed solution of a carbon source and a nitrogen source, the content of the brevibacitracin A in fermentation liquor is further greatly increased and reaches 7.5 g / L or above, and the brevibacitracin A can be used for large-scale industrial production.

The invention discloses a process for removing pollutants in industrial aquaculture tail water of penaeus vannamei boone, and belongs to the technical field of aquaculture tail water treatment. Comprising the following steps: (1) filtering breeding wastewater to remove solids; (2) compound bacteria are added into the filtered breeding wastewater for aeration treatment, aerated wastewater is obtained, and the compound bacteria comprise rhodococcus ruber, bacillus subtilis, brevibacterium halotolerans and photosynthetic bacteria according to the weight ratio of (1-2): (2-3): (3-4): (0.8-1.5); (3) adding a flocculating agent into the aerated wastewater, treating at 45-50 DEG C for 0.5-1 hour, and filtering to obtain a first filtrate; (3) adding modified activated carbon into the first filtrate, treating at 45-50 DEG C for 1-2 hours, and filtering to obtain a second filtrate; and (4) treating the second filtrate through mixed macroporous adsorption resin to obtain a third filtrate, and sterilizing the third filtrate. By combining physical, chemical and biological treatment, pollutants in the penaeus vannamei culture tail water are efficiently removed.

This invention enables accurate measurement of greenhouse gas emissions from animals. It also allows for easy evaluation of the reduction effect of greenhouse gas emissions from animals. Furthermore, it provides a methane emission inhibitor that suppresses methane production from Methanobrevibacter bacteria. [Solution] A bacterial quantification method for quantifying Methanobrevibacter species contained in the rectal feces of animals includes the steps of: collecting rectal feces from an animal; holding the rectal fecal sample in an anaerobic porter under anaerobic conditions; replacing the gas in the anaerobic porter with nitrogen gas, diluting the sample with a culture medium, and culturing the bacteria in a test tube; recovering the cultured bacteria; extracting DNA from the recovered bacteria; and quantifying the Methanobrevibacter species from the extracted DNA by quantitative PCR or a gene analysis method using next-generation sequencing analysis.

The present disclosure provides a method for culture of bacterial strains, comprising the following steps: inoculating a bacterial strain in a first culture medium comprising 0.1 mg / L-0.8 mg / L of 1-bromohexane to obtain a first culture. The method for culture of bacterial strains provided by the present disclosure further comprises: inoculating the first culture into a second culture medium, wherein the second culture medium comprises 0.2 mg / L-0.8 mg / L of 1-bromohexane. The method can be used for fermentation of Gluconacetobacter melanogenum, Propionibacterium, Corynebacterium, Brevibacterium, Bacillus, Gluconobacter, Lactobacillus, Agromyces, Actinobacillus, Escherichia coli, Anaerobiospirillum, Trichoderma, Streptomyces, Aspergillus, Saccharomyces or Myceliopthora, effectively preventing infection of bacteriophages during fermentation.

The invention relates to the technical field of organic pollutant treatment, in particular to a method for degrading aged petroleum hydrocarbon pollutants in soil. Firstly, low-temperature dielectric barrier plasma treatment with specific parameters is utilized, and a preliminary degradation effect is performed according to the characteristics of the aged petroleum hydrocarbon pollutants; and secondly, by utilizing specific types of compound bacteria microorganisms and by virtue of a good synergistic effect among the pseudomonas aeruginosa, the microbacterium oxydans and the ochrobactrum intermedium in the compound bacteria microorganisms, the degradation efficiency and the degradation rate of the microorganisms on the primary degradation substances are obviously improved, and an intermediate degradation product with smaller molecular weight is generated. In addition, after the compound bacteria microorganisms are introduced, through control of specific conditions, deep degradation of the compound bacteria microorganisms on intermediate degradation products is achieved, and the total degradation rate is greatly increased. Through low-temperature dielectric barrier plasma treatment and'different space coupling 'of compound bacteria and microorganisms, a new idea is provided for treating pollutants through the coupling synergistic effect of the low-temperature dielectric barrier plasma treatment and the compound bacteria and microorganisms.

This invention enables accurate measurement of greenhouse gas emissions from animals. It also allows for easy evaluation of the reduction effect of greenhouse gas emissions from animals. Furthermore, it provides a methane emission inhibitor that suppresses methane production from Methanobrevibacter bacteria. [Solution] A bacterial quantification method for quantifying Methanobrevibacter species contained in the rectal feces of animals includes the steps of: collecting rectal feces from an animal; holding the rectal fecal sample in an anaerobic porter under anaerobic conditions; replacing the gas in the anaerobic porter with nitrogen gas, diluting the sample with a culture medium, and culturing the bacteria in a test tube; recovering the cultured bacteria; extracting DNA from the recovered bacteria; and quantifying the Methanobrevibacter species from the extracted DNA by quantitative PCR or a gene analysis method using next-generation sequencing analysis.