150 results about "Brevibacterium" patented technology

The curative action of drugs, including herbal remedies, allopathic remedies, and periodontal remedies, is enhanced and accelerated by administering such drugs in combination or association with probiotics, especially those of genus Lactococcus, Lactobacillus, Pediococcus, Streptococcus, Propionibacterium, Brevibacterium, Penicillium, and Saccharomyces.

The invention relates pseudomonas, use thereof and a method for removing cadmium pollution to the environment. The pseudomonas class is named pseudomonas putidaHN103 and the collection number in a collection center is CCTCCNo.M2011184; and the shape and characteristics of the pseudomonas putidaHN103 comprise: 1) on a culture medium, a bacterial colony which used to be colorless and transparent turns white, smooth and convex-like with irregular edges; and 2) according to the result of bacterial microscopic examination, pseudomonas belongs to brevibacterium and is gram negative. The use of the bacteria is the use of the bacteria as a biological repair material for removing cadmium pollution. The method for removing cadmium pollution to the environment comprises the following steps: 1) regulating the cadmium ion concentration in sewage to a range in which pseudomonas can grow normally; 2) placing pseudomonas; 3) keeping the pseudomonas in the sewage for a certain time period; and 4) removing bacterial absorbing cadmium ions. The pseudomonas requires small investment, is low in cost, high in adaptability and capable of effectively removing cadmium, can quickly and obviously lower the concentration of cadmium ions in the environment and is suitable for biological remediation environments polluted by cadmium.

The invention discloses an ultrahigh temperature decomposition agent of straws and cattle manure, and a preparation and an application of the decomposition agent, and belongs to the field of decomposition agents. The decomposition agent is prepared by mixed fermentation and drying of the straw, the cattle manure and a complex decomposition inoculant, wherein the complex decomposition inoculant comprises 1-10 parts of thermophilic bacterium inoculant, 1-6 parts of bacillus licheniformis inoculant, 1-6 parts of aspergillus oryzae inoculant, 1-6 parts of lactobacillus plantarum inoculant, 1-6 parts of thermoactinomyces sacchari, and 1-6 parts of cold-proof brevibacterium inoculant. The decomposition agent, a composting method and the application of the decomposition agent solve the problems of slow temperature rise during composting and decomposition, low fermentation temperature, long fermentation time, incomplete decomposition, poor compost quality and the like in the fermentation of the existing complex inoculant, and the decomposition agent is quick in temperature rise during the composting and the decomposition, quick in heating-up, high in fermentation temperature, short in fermentation time, more thorough in decomposition and higher in quality.

The invention relates to a strain capable of producing L-arginine and a method for producing the L-arginine by the strain and belongs to the technical field of biological engineering. For the strain, Brevibacterium flavum ATCC 14067 is used as a starting strain; nitrosoguanidine is adopted to carry out mutagenesis step by step; mutant strains with histidine and succinic acid auxotrophic strains are screened out so as to cut off a competitive metabolic pathway; and mutant strains (His-, Suc-, D-Argr, SMCr) with resistances of arginine structural analogs and cysteine structural analogs are screened out. The strain is named as Brevibacterium flavum HX1009, is preserved in the China general microbiological culture collection center, has the preservation number of CGMCC No.4464 and has the genetic characters of histidine auxotroph His-, succinic acid auxotroph Suc-, D-arginine resistance D-Argr and S-methyl cysteine resistance SMCr for improving the yield of the L-arginine. Under the optimized condition, the L-arginine is produced by fermentation on a fermentation tank with the volume of 5L to 5M3 and the arginine production level achieves 50 to 70g/L.

The invention relates to a recombinant DNA (deoxyribonucleic acid), strain and method for producing L-valine by fermentation. The recombinant DNA comprises DNA sequence for coding acetohydroxy acid synthetase free of feedback inhibition of three branched-chain amino acids to acetohydroxy acid synthetase, DNA sequence for coding dihydroxy reduction isomerase, and DNA sequence for coding branched-chain amino acid aminotransferase. The strain is corynebacterium or brevibacterium transformed by introducing the recombinant DNA. The method is implemented by culturing the strain to produce the L-valine. In the invention, the expression L-valine is used for producing the bacterium L-valine biosynthetic gene, so the yield of the L-valine can be greatly increased; and the fermentation model, especially the high-temperature short-time fermentation model, is constructed according to the optimum temperature of the L-valine biosynthetic enzyme, so that the metabolism intensity of the strain is enhanced, the activity of the L-valine biosynthetic enzyme is enhanced, and the yield of the L-valine and the conversion rate of the sugar acid can be further increased.

The invention discloses a 40% weaned piglet concentrate feed. Components comprise dehulled soybean meal, zymolytic plant small peptide, fish meal, choline chloride, acidifying agents, flavoring agent, tasty agents, suckling pig composite vitamin, suckling pig composite organic microelements, lysine, methionine, probiotics (lactic acid bacteria, saccharomycetes and brevibacterium bacteria), calcium hydrophosphate, vegetable oil and mountain flour. The feed is convenient to use; and corn flour is added and mixed evenly according to a recommended formula, so that a nursery pig complete formula feed can be prepared, nutrient requirements of nursery pigs are met completely, piglet growth potentials can be fully displayed, and economic benefits are improved. According to the feed, a low dosage of antibiotics which are dedicated for animals and cannot be absorbed is used, food safety is guaranteed, and the feed utilizes organic microelements with high bioavailability and does not contain high calcium, phosphorus, copper and zinc, so that the feed is environment friendly.

The invention discloses a small brevibacterium strain capable of carrying out biological denitrification under the high-salt condition and application of the small brevibacterium strain. The small brevibacterium strain provided by the invention is preserved in China General Microbiological Culture Collection Center (CGMCC for short) on March 29th, 2012, with the preservation number of CGMCC No.5947. At the salinity (counted by NaCl) is 3-10 percent, the strain is added into nitrogen-containing wastewater with dissolved oxygen of 2-6mg/L for biological denitrification. The strain has strong resistant ability for high salinity and heterotrophic nitrification and aerobic denitrification capabilities and can better grow under the high-salinity condition. Under the high-salinity and aerobic conditions, the strain can be used for synchronous nitrification and denitrification and efficiently and thoroughly removing total nitrogen in sewage, and thus the major problem that biological denitrification is difficult to carry out under the high-salinity condition is effectively solved and a favorable application prospect is obtained.

The invention relates to bacteria capable of eliminating ammonian in micro-polluted source water under microthermal and aerobic condition and a screening and domestication method, relating to bacteria and screening and domestication method and solving the problem that the existing bacteria are not suitable for the treatment of the micro-polluted source water and low temperature treatment. The bacterium SRA 13 of the invention is Brevibacterium mcbrellneri, belongs to brevibacterium and is collected in the CGMCC, the collection number is CGMCC No. 2890, and the collection date is 19th, January 2009. The screening method includes the steps as follows: first, separation and purification; second, bacteria solution culture; third, the screening and the culture of the bacteria solution of survival strains; fifth, the progressive low-temperature domestication of the survival strains after the repetition of the step fourth; and sixth, the acquisition of bacteria solution of the survival strains after the low-temperature domestication, and secondary screening. The bacteria of the invention can treat the micro-polluted source water under the condition of low temperature of 2 to 10 DEG C.

An immunomodulator having suppressive activity on IgE antibody production is provided and contains bacterial cells, or their decomposition materials. It can be taken as a food. Bacterial cells such as Corynebacterium, Brevibacterium, Microbacterium, or bacterial cells of mutant strains of these bacteria, or decomposition products of these bacteria are used.

The invention relates to a denitrifying phosphate-accumulating organism with nitrogen and phosphorus removal functions and applications thereof. The strain is named as pseudomonas pseudoalcaligenes CL-1, belongs to pseudomonas pseudoalcaligenes, and is preserved in China Center for Type Culture Collection on June 13, 2012, and the preservation numbers is CCTCC NO: M2012225. The strain is gram-positive and belongs to brevibacterium, a bacterial colony is rounded, milk white, convex and glossy, and edges are smooth and transparent. The growth cycle of the organism is long, the nitrogen and phosphorus removal rates in the first 24h are respectively 56.93% and 43.56%, and the nitrogen and phosphorus removal rates in the whole growth cycle are respectively 75.6% and 57.5%. The strain has a complete denitrifying capacity, and can directly reduce inorganic nitrogen such as nitrate nitrogen and nitrite nitrogen and the like to harmless nitrogen gas discharged from water bodies. When the denitrifying phosphate-accumulating organism is used for treating nitrogen and phosphorus containing wastewater, the process is simple, the treatment effect is efficient and stable, and the operating cost is saved, meanwhile, no greenhouse gas such as NO and N2O and the like is produced, therefore, no air pollution is caused.

The invention discloses a method for preparing lysine-rich fermented soybean meal, which is simple and can effectively improve the content of nutrients of the soybean meal. The method is technically characterized by comprising the following steps: (1) preparing strains: a, inoculating the brevibacterium flavum into the brevibacterium flavum liquid culture medium, and vibrating and culturing at 28-37 DEG C to obtain the first-level brevibacterium flavum strain when the OD590 value of the brevibacterium flavum liquid is 0.25-0.30; b, the inoculating the saccharomycetes into the saccharomycetes liquid culture medium, and vibrating and culturing at 28-37 DEG C to obtain the first-level saccharomycetes strain when the OD590 value of the saccharomycetes liquid is 0.25-0.30; and c, inoculating the lactobacillus into the lactobacillus liquid culture medium, and vibrating and culturing at 30-37 DEG C to obtain the first-level lactobacillus strain when the OD590 value of the lactobacillus liquid is 0.25-0.30; (2) preparing a fermentation substrate; and (3) inoculating the fermentation substrate. The method for preparing the lysine-rich fermented soybean meal belongs to the technical field of feed preparation.

The invention discloses a biological enzyme treating agent of industrial sewage. The biological enzyme treating agent is characterized by comprising, by weight, 10 parts to 18 parts of oxidation-reduction enzymes, 10 parts to 15 parts of cellulose, 5 parts to 8 parts of protease, 5 parts to 8 parts of laccase, 6 parts to 8 parts of methanogenesis brevibacterium, 6 parts to 8 parts of nitrococcus, 0.5 parts to 1.5 parts of bamboo juice, and the balance water. According to the biological agents conduct disposing on sewage of printing and dyeing mills and similar industries through a biological catalysis technology which has a biological degradation function, foul smell can be dispelled greatly so as to degrade liquid pollutants rapidly and reduce the size of solid substances, polluted substances can be purified rapidly, treatment effect is good, operating cost of sewage treatment can be reduced greatly, the purified water is stable in water quality, the biological enzyme treating agent of the industrial sewage is energy-saving, efficient and capable of treating pollution, and has notable economical benefits and environmental benefits.

The invention discloses a brevibacterium halotolerans with relatively high azotase activity and application thereof in ecological restoration. The brevibacterium halotolerans GDSD21 is collected in the China General Microbiological Culture Collection Center with the collection number of CGMCC No.12402. Nitrogen-fixing bacteria are separated from rhizosphere soils of field-crop roots, further strains with relatively high azotase activity are screened, and finally the efficient nitrogen-fixing bacterium brevibacterium halotolerans GDSD21 is screened. The azotase activity of the brevibacterium halotolerans GDSD21 is obviously higher than that of common production strain azotobacter chroococcum ACCC11103 for microbial fertilizers, and the brevibacterium halotolerans GDSD21 has extensive application prospects in soil improvement, increasing of the soil fertility, ecological restoration of a virgin land, and production of industrialized seedling-rearing inoculation agents, nitrogen-fixing microbial agents and bio-organic fertilizers for vegetables.

Relating to the field of microorganisms, the invention discloses an inoculant, a feed or additive and a removal method for mycotoxins. The invention specifically discloses an inoculant, which includesat least two of mould, yeast and bacteria, wherein the bacteria can be at least one of brevibacterium, acinetobacter, rhodococcus and pseudomonas. Specifically, the inoculant can remove mycotoxins. The invention also discloses application of the inoculant in removal of mycotoxins, an additive containing the inoculant and a removal method for mycotoxins. According to the technical scheme, combineduse of at least two of the yeast, mould and bacteria can achieve simultaneous removal of ochratoxin A, fumonisins and T-2 toxin, and the removal efficiency of ochratoxin A and fumonisins is the highest.

The invention relates to a straw treatment process, in particular to a pretreatment method for densifying rice and wheat straw solid briquette fuel. The pretreatment method for densifying the rice and wheat straw solid briquette fuel comprises the following steps of: grinding straws with moisture content of less than or equal to 12 weight percent; adding a small amount of ammonia water or/and quick lime into water to prepare a spraying solution, wherein the amount of the ammonia water or/and the quick lime is 1 to 2 percent of the total amount of the spraying solution; spraying the prepared spraying solution on ground straw materials so as to wet the ground straw materials; placing the wet ground straw materials into a fermentation tank to ferment for 8 to 12 hours; and adding potassium chlorate or/and potassium nitrate into the fermented ground straw materials and uniformly stirring, wherein the adding amount of the potassium chlorate or/and the potassium nitrate is 2 to 3 percent of the total amount of the ground straw materials. Oxytolerant and alkaline-resisting cellulose decomposing brevibacterium domesticated manually can also be added into the spraying solution and the adding amount of the oxytolerant and alkaline-resisting cellulose decomposing brevibacterium is 0.5 to 1.5 percent of the total amount of the spraying solution. By the pretreatment method, original structural ingredients of a straw material can be effectively changed, the carbon element is kept, the caking property is improved, a hydrogen bond in the straws is destroyed (so that the straws are of a regular structure), cellulose in the straws is partly degraded and compact waxiness contained on the surfaces of the straws is decomposed, so that the straws are softened and are easy to process and form.

The invention discloses a microbial fertilizer for accelerating crop growth and a preparation method for the microbial fertilizer. The microbial fertilizer comprises the components in parts by mass: 100 parts of plant straws, 8-16 parts of rhamnolipid, 4-10 parts of nucleotide salt, 60-80 parts of chitosan hydrochloride, 0.8-1.2 parts of a bacillus subtilis fungicide, 6-10 parts of lactic acid bacteria powder, 4-8 parts of saccharomycetes powder, 8-20 parts of monopotassium phosphate, 6-10 parts of ferrous sulfate, 10-15 parts of calcium carbonate, 1-3 parts of magnesium chloride, 1-3 parts of ammonium molybdate, 1-3 parts of zinc sulfate, 1-3 parts of manganese sulfate and 1-3 parts of boric acid. The rhamnolipid and the nucleotide salt in the microbial fertilizer have mechanisms of promoting growth and play a synergistic role if being compounded in use, so that the growth promotion to the plant is remarkably improved; in addition, the rhamnolipid further can improve the degradation rate of plant straw fibers by bacillus subtilis with brevibacterium. In addition, the chitosan hydrochloride can degrade plant and animal residues and trace metal elements and convert those into nutrients of plants so as to promote health of the plants.

The invention relates to a new strain golden Brevibacterium strain AN3 CGMCC No.2193, and application thereof in degrading triphenyl methane dyes and azo dyes such as malachite green. The golden Brevibacterium strain AN3 is obtained by separating and purifying activated sludge in an anaerobic baffled reactor system used for treating printing and dyeing wastewater by an research institute in Guangzhou, can degrade the triphenyl methane dyes in a mode of benzene ring detoxication under a facultative anaerobic condition that the temperature is between 25 and 37 DEG C and the pH value is between 6.0 and 8.5, and has significant capability on degrading the prior triphenyl methane dyes and azo dyes such as the malachite green which bring about serious pollution to aquatic products and are used in the form of a fungicide, wherein the removal rate of the golden Brevibacterium strain to the malachite green with the concentration of 50 milligrams per liter is 61.4 percent in 8 hours and is 97.3 percent in 16 hours, and the highest tolerable concentration thereof is 500 milligrams per liter, so that the strain golden Brevibacterium strain can effectively solve the problems of pollution and purification caused by the malachite green in an aquaculture environment.

The invention relates to brachybacterium nesterenkovi new strain and its culturing condition, physiological biochemical characteristic, and enzyme manufacturing method. It includes the following steps: preparing soil solution; gradient diluting; coating the given 200ul solution to 30ul beef extract culture medium; adding 100ul-800ul alcohol inducer ^[12-14]; culturing at 30-40 centigrade degree for 16-48h; purifying for many times; it is single cloning strain by microscopic examination; liquid fermenting; ultrasonic wave smashing; centrifugal separating; purifying; and testing its activity. The advantages of the invention are that the strain can produce acetadehyde dehydrogenase and alcohol dehydrogenase; its culturing condition is simple and low cost to be good for industrialization production; it has high activity at 37 centigrade degree and can impel alcohol to metabolize in human body.

The invention discloses a high-yield production method of GABA (gamma amino butyric acid) through mixed fermentation of brevibacterium tianjinese and lactobacillus plantarum, and belongs to the fields of fermentation engineering and enzyme engineering. According to the invention, glucose is transformed into glutamic acid by using the brevibacterium tianjinese SW07-1, and then glutamic acid of fermentation liquor obtained in the last step is transformed into GABA by using the lactobacillus plantarum GB01-21. Before transformation, the pH value of glutamic acid fermentation liquor is adjusted to be the optimum pH value of glutamic acid decarboxylase, so that a transformation system is built, and in the step 2, after the fermentation liquor is filtrated or centrifuged, the lactobacillus plantarum GB01-21 is obtained, and then the lactobacillus plantarum GB01-21 is added into the transformation system to carry out whole-cell transformation. When original glutamic acid of the fermentation liquor is completely transformed, GABA in the transformation liquor is 59.2 g/L, and the molar transformation rate is 93.6%. After an exogenous glutamic acid is continued to be added and then transformed, the GABA content is 96.5 g/L, and the molar transformation rate is 91.8%.

The present invention discloses one kind of Bacillus alcaligenes and the process of preparing single antimer mandelic acid therewith. The resting cell of the Bacillus alcaligenes, ECU0401 CGMCC No.2009, is used as the biological catalyst in selectively converting racemized mandelic acid and its derivative to prepare optically active mandelic acid and its derivative. The Bacillus alcaligenes strain of the present invention has high catalyzing effect, and the product (R)-(-)-mandelic acid has optical purity over 99.9 %, obviously higher than that obtained with catalyzing yellow Bacillus brevis AS1.818 and with catalyzing Saccharomyces cervisiae AS2.150.

The invention relates to a preparation method of peanut soy, belonging to the technical field of preparation methods of soy. The peanut soy provided by the invention adopts groundnut kernels as a raw material, bacillus licheniformis, brevibacterium flavum, clostridium acetobutylicum and rhamnose lactobacilli are taken as composite fermentative bacteria of a peanut fungi solution; the special proportional aspergillus oryzae, aspergillus niger and monascus are utilized as fermenting aspergillus of a peanut yeast, and a preparation process of natto soy is adopted. The preparation method provided by the invention has the advantages that the fermentation period is shortened, natto kinase is generated after fermenting of groundnut kernels, the kinase can restrain the browning of the soy in preservation, a maillard reaction is reduced, the nutritive values of the groundnut kernels are high, the flavor is unique, and the color, the aroma and the taste of the prepared soy are good; the content amino acid nitrogen is improved by 43.9%, the full nitrogen content is improved by 17.5%, a soluble salt-free solid is improved by 18.8%, and the preparation method is especially used for preparing ideal soy of pickles.

The invention relates to an Acidiphilium spp.DX-X and uses thereof, the strain can be cultured in a BSYG liquid culture medium and solid culture medium, and can be grown advantageously within a temperature range from 25 to 40 DEG C and within a pH range from 2.5 to 4.5. The strain is Gram-negative bacterium which is in Brevibacterium, capable of moving and solitary, belongs toacidophilic microorganism, and can be grown on condition of 3% of salt concentration. The strain can not utilize sulfur powders and sodium thiosulfate as well as can not regard Fe as an energy source. Glucose, mannitol and glycerol can be utilized as organic energy sources, oxidase is negative while contact enzyme is positive. The bacterium promotes the growth of Thiobacillus ferrooxidans on the solid culture medium, and has, when implementing a mixed leaching on Jinchuan nickel mine with the Thiobacillus ferrooxidans, a leaching rate superior to that of the Thiobacillus ferrooxidans at the time of leaching individually, therefore, the bacterium possesses enormous potential in case of being applied to bio-hydrometallurgy industry.

This invetion relates to Pullulan membrane and its use for culture preservation. The said membrane is prepared by flow spreading process to form thickness 0.02-0.04mm, pull strength 27-32MPa, right tearing strength 31-38KN/m, light transmittance rate 95-98%, and oxygen penetration (2.7-1.6cm3/m2.bar.d). The method is carried out by: providing glycerin, trehalose as protecting agent and microbe between both of membranes, pressing tightly and sealing with plastic layers to meet conditions for culture preservation, e.g., dryness, oxygen depletion, low temperature, nutrient depletion and protecting agent addition. Those sheets can be formed into albums for preservation, research and easy reproduction by cutting a piece and putting into a culture dish. No expensive apparatus and large space for preservation are needed and it is easily posted.

The invention discloses ocean-sourced Microbacterium keratanolyticum MCDA02 with the collection number of CGMCC NO.13539. The strain is Gram-negative spore-free brevibacterium, and after being cultured for 48 h on a 2216E solid culture medium, the strain is yellow, translucent, smooth and moist in surface, circular, neat in edge, slightly protruding in center and easy to pick up. The strain grows slowly at 5 DEG C and 45 DEG C, and the optimal growth temperature is 30 DEG C; the growth pH range is 6.0-9.0, and the optimal growth pH is 8.0; and when the NaCl concentration is 0-10%, the strain can grow, and the optimal growth NaCl concentration is 3%. The invention further discloses a method for producing chitin deacetylase by using the strain MCDA02 and a product. For the chitin deacetylase produced by the Microbacterium keratanolyticum MCDA02 strain provided by the invention, the optimal pH is 8.0 and the optimal temperature is 30 DEG C; and at a low temperature, the chitin deacetylase has relatively high activity, so that energy can be saved and the cost can be reduced in industrial application.