26 results about "Brevibacterium" patented technology

The invention relates to the technical field of food microorganisms, fermentation and seasonings, in particular to a natural fermentation flavor enhancer as well as a preparation method and application thereof. The natural fermentation flavor enhancer comprises 3-40 wt% of glutamic acid of a natural source and 0.2-25 wt% of IMP and / or GMP of a natural source. Wherein the natural fermented flavor enhancer is obtained by a method comprising fermentation by using microorganisms, and the microorganisms are selected from two or more of yeast, lactic acid bacteria, brevibacterium ammoniacum and corynebacterium glutamicum and at least comprise one of yeast or lactic acid bacteria. The natural fermented flavor enhancer is obtained through mixed fermentation of microorganisms, the flavor and taste of the flavor enhancer are improved, and astringency and peculiar smell of the product are eliminated; the natural substrate is adopted for fermentation, no additive is contained, and the requirements for greenness, safety and health are met.

The present application relates to the field of microbial technology, and specifically discloses a strain of Brachybacterium paraconglomeratum 2-1 and its application in industrial wastewater treatment; the Brachybacterium paraconglomeratum 2-1 is preserved in the China General Microbiological Culture Collection Center on May 29, 2025, with a preservation number of CGMCC No.34719 and a classification name of Brachybacterium paraconglomeratum; the strain 2-1 can tolerate the high toxicity environment of residual ammonia water in a coking plant, has a good effect in the microbial treatment of industrial wastewater containing phenol, can grow by taking phenol as the only carbon source, realizes the removal of phenol in wastewater under aerobic conditions, and has great application potential in the biological treatment of industrial wastewater.

Provided herein are methods for treating or preventing recurrent Clostridioides difficile infection, comprising bacterial strains belonging to species Clostridium bolteae, Anaerotruncus colihominis, and / or Flavonifractor plautii. Also provided are compositions comprising bacterial mixtures of strains including Clostridium bolteae, Anaerotruncus colihominis, Sellimonas intestinalis, Clostridium symbiostim, Blautia producta, Dorea longicatena, Clostridium innocuum, Flavonifractor plautii, Lachnospiraceae bacterium, and / or Bifidobacterium longum. Also provided are methods of using compositions comprising such bacterial mixtures (e.g., for treating or preventing Clostridium difficile infection).

The present application relates to the field of microorganisms and biotechnology, and particularly relates to a Brevibacillus parafacis and application thereof in degrading polyphenols in tobacco leaves. The Brevibacillus parafacis is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 63066, and the preservation date is December 20, 2022. The strain can effectively degrade the content of polyphenols in cigar tobacco leaves, especially chlorogenic acid.

The invention provides a disease-preventing and growth-promoting compound microbial agent special for traditional Chinese medicinal materials as well as a preparation method and application of the compound microbial agent, and belongs to the technical field of biocontrol microbial agents. The disease-preventing and growth-promoting compound microbial agent special for the traditional Chinese medicinal materials, disclosed by the invention, is prepared from cellulomicrobium fennellii MHP3, brevibacterium brevicola MHP56, bacillus tequilensis MHP82 and bacillus cereus MHP107. The disease-preventing and growth-promoting compound microbial agent disclosed by the invention has a relatively good prevention and treatment effect on root rot of traditional Chinese medicinal materials, and also can promote the growth of the traditional Chinese medicinal materials and increase the yield. The disease-preventing and growth-promoting compound microbial agent disclosed by the invention can also improve rhizosphere soil of traditional Chinese medicinal materials and reduce the salt and alkali content in the soil.

The invention discloses a microbial agent for improving disease resistance of tea trees and a preparation method of the microbial agent, and belongs to the field of agricultural disease prevention and control. According to the invention, the optimal strain compounding condition is obtained by screening the strain compounding condition, and experiments prove that the fungicide prepared when the brevibacterium Hainan powder ACCC 60371 and the streptomyces griseus var rust CICC 23624 are compounded according to the mass ratio of 2: 5 can enable the prevention and treatment effects of tea leaf blight and zonate spot to reach 88.58% and 84.41% respectively, so that the fungicide can be used for preventing and treating the tea leaf blight and zonate spot respectively. And the polyphenol content is obviously superior to the effect of preventing and treating the two tea tree diseases by single bacteria. The invention provides a strain source and a method for improving disease resistance and tea quality.

The application discloses a process for removing pollutants in factory breeding tail water of penaeus vannamei, and belongs to the technical field of breeding tail water treatment. The process comprises the following steps: (1) filtering breeding wastewater to remove solid matters; (2) adding composite bacteria to the filtered breeding wastewater for aeration treatment to obtain aeration wastewater, wherein the composite bacteria comprise, by weight, 1-2 parts of rhodococcus, 2-3 parts of bacillus subtilis, 3-4 parts of halococcus and 0.8-1.5 parts of photosynthetic bacteria; (3) adding a flocculating agent to the aeration wastewater, treating the aeration wastewater at 45-50 DEG C for 0.5-1 h, and filtering to obtain a first filtrate; (4) adding modified activated carbon to the first filtrate, treating the first filtrate at 45-50 DEG C for 1-2 h, filtering to obtain a second filtrate; and (5) flowing and mixing the second filtrate through a macroporous adsorption resin to obtain a third filtrate, and sterilizing the third filtrate. The application realizes efficient removal of pollutants in the breeding tail water of penaeus vannamei through the combination of physical, chemical and biological treatments.

The invention provides a marine sediment pumilus Thr32 strain and application thereof. The preservation number of the Thr32 strain is GDMCC No: 67315. The invention further provides a preparation method of the marine sediment pumilus Thr32 strain. The application of the marine sediment brevibacterium in prevention and treatment of growth of Orobanche cucurbitae is reported for the first time, and the types of strains for prevention and treatment of growth of Orobanche cucurbitae are enriched. According to the application of the Curtobacterium crenatum Thr32 provided by the invention in prevention and treatment of Orobanche cucurbitae, the inhibition rate of separated strain fermentation liquor on seed germination of Orobanche cucurbitae is as high as 100%, and the method has a wide application value in biological prevention and treatment of Orobanche cucurbitae.

The invention provides bacillus brevis HDCC00067, the preservation number of the bacillus brevis HDCC00067 is CGMCC (China General Microbiological Culture Collection Center) No.35553, and the capability of producing brevibacterium peptide A is obviously higher than that of the prior art. The applicant also finds that the yield of the brevibacitracin A can be obviously improved when amino acid precursor substances are added into the fermentation culture medium, especially when the concentrations of L-tryptophan, D-leucine and D-valine are controlled to be within a certain range. By controlling pH and dissolved oxygen in a tank fermentation culture medium and optimizing a feeding control strategy of a mixed solution of a carbon source and a nitrogen source, the content of the brevibacitracin A in fermentation liquor is further greatly increased and reaches 7.5 g / L or above, and the brevibacitracin A can be used for large-scale industrial production.

The invention discloses a process for removing pollutants in industrial aquaculture tail water of penaeus vannamei boone, and belongs to the technical field of aquaculture tail water treatment. Comprising the following steps: (1) filtering breeding wastewater to remove solids; (2) compound bacteria are added into the filtered breeding wastewater for aeration treatment, aerated wastewater is obtained, and the compound bacteria comprise rhodococcus ruber, bacillus subtilis, brevibacterium halotolerans and photosynthetic bacteria according to the weight ratio of (1-2): (2-3): (3-4): (0.8-1.5); (3) adding a flocculating agent into the aerated wastewater, treating at 45-50 DEG C for 0.5-1 hour, and filtering to obtain a first filtrate; (3) adding modified activated carbon into the first filtrate, treating at 45-50 DEG C for 1-2 hours, and filtering to obtain a second filtrate; and (4) treating the second filtrate through mixed macroporous adsorption resin to obtain a third filtrate, and sterilizing the third filtrate. By combining physical, chemical and biological treatment, pollutants in the penaeus vannamei culture tail water are efficiently removed.

This invention enables accurate measurement of greenhouse gas emissions from animals. It also allows for easy evaluation of the reduction effect of greenhouse gas emissions from animals. Furthermore, it provides a methane emission inhibitor that suppresses methane production from Methanobrevibacter bacteria. [Solution] A bacterial quantification method for quantifying Methanobrevibacter species contained in the rectal feces of animals includes the steps of: collecting rectal feces from an animal; holding the rectal fecal sample in an anaerobic porter under anaerobic conditions; replacing the gas in the anaerobic porter with nitrogen gas, diluting the sample with a culture medium, and culturing the bacteria in a test tube; recovering the cultured bacteria; extracting DNA from the recovered bacteria; and quantifying the Methanobrevibacter species from the extracted DNA by quantitative PCR or a gene analysis method using next-generation sequencing analysis.

The invention discloses a method for improving the fermentation yield of L-valine and the conversion rate of saccharic acid, and relates to the technical field of bioengineering, and the method comprises the steps of strain modification, preparation and addition of a composite fermentation synergist, a photo-thermal synergistic fermentation strategy, construction of an intelligent fermentation regulation and control system and the like. Corynebacterium glutamicum or brevibacterium flavum is selected as a fermentation strain, and a specific metabolic regulation solution is added to induce the strain to secrete signal molecules, so that the activity of key enzymes for synthesizing L-valine is enhanced, and byproducts are reduced; a compound fermentation synergist is prepared and added, and basic fermentation conditions are optimized; strategies such as light-magnetic synergistic fermentation and pulse-intermittent ventilation are adopted, dynamic regulation and control are achieved in cooperation with an intelligent fermentation regulation and control system, the fermentation yield of L-valine and the sugar-acid conversion rate are remarkably increased, and the production efficiency and economic benefits are improved.

The invention relates to the technical field of microorganisms, and particularly discloses a brevibacterium paragglutinatus 2-1 and application thereof in industrial wastewater treatment. The brevibacterium paracareactor 2-1 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on May 29, 2025, the preservation number is CGMCC No.34719, the brevibacterium paracareactor 2-1 is classified and named as brevibacterium paracareactor, the preservation number is CGMCC No.34719, the preservation number is CGMCC No.34719, the preservation number is CGMCC No.34719, the preservation number is CGMCC No.34719, the preservation number is CGMCC No.34719, the preservation number is CGMCC No.34719, the preservation number is CGMCC No.34719, and the preservation number is CGMCC No.34719. The strain 2-1 can tolerate a high-toxicity environment of residual ammonia water of a coking plant, has a good effect in the aspect of microbiological treatment of phenol-containing industrial sewage, can grow by taking phenol as a unique carbon source, realizes removal of phenol in wastewater under an aerobic condition, and has huge application potential in biological treatment of industrial wastewater.

The present invention discloses a composite bacterial agent for promoting decomposition and retaining nitrogen at low temperature composting, and a preparation method and application thereof. The active ingredients of the composite bacterial agent are composed of Providencia alcalifaciens CCTCC No: M 2022672 and Brevibacterium frigoritolerans CCTCC No: M 2022673. The cultured composite bacterial agent is mixed with a carrier to obtain a solid composite bacterial agent. The solid composite bacterial agent can be applied to the composting process of agricultural waste in a low temperature environment to promote the conversion of organic matter and reduce nitrogen loss, thereby improving the quality of compost.

The invention discloses a biological agent for rapidly decomposing straws in a cold area as well as a preparation method and application of the biological agent, and belongs to the technical field of microbial preparations. The invention relates to a biological agent for rapidly decomposing straws in a cold area. The biological agent is prepared from brevibacterium tumefaciens CGMCC (China General Microbiological Culture Collection Center) 1.10488, pichia pastoris CICC 32806, trichoderma longibrachiatum CICC 13054, aspergillus niger CICC 2151 and a carrier. The biological agent disclosed by the invention has the advantages of high cold resistance, high activity, high degradation speed and the like, has relatively high metabolic capability at low temperature, is suitable for straw decomposition treatment in cold and cool areas, and provides a new technical thought for crop product management in low-temperature areas.

The invention discloses a special alpine medicinal plant cultivation microbial fertilizer for alpine regions, and relates to the technical field of fertilizers. The microbial fertilizer for cultivation of the medicinal plants in high and cold areas is prepared from the following raw material components in parts by mass: 8 to 12 parts of humic acid, 2 to 4 parts of wormcast, 8 to 15 parts of sepiolite, 3 to 5 parts of modified biomass charcoal, 3 to 4 parts of a chenopodium glaucum root extract, 0.5 to 1.0 part of a cedarwood extract and 0.4 to 0.8 part of compound microorganisms, the compound microorganism is a mixture compounded by cold-resistant bacillus subtilis and brevibacterium brevicola according to the mass ratio of 3: 1. The microbial fertilizer disclosed by the invention can meet the nutritional requirements of the high-cold medicinal plants in a severe environment, the used raw materials are reasonably matched, and the microbial fertilizer effectively ensures the yield and quality of the high-cold medicinal plants and has important economic value in the field of planting of the high-cold medicinal plants.

The present invention discloses a cold-tolerant Brevibacterium and its application in the treatment of livestock and poultry manure in the development and utilization of biological strains. The cold-tolerant Brevibacterium TW009 was deposited at the China Center for Type Culture Collection on June 1, 2022, and was classified and named Brevibacterium frigoritolerans TW009, with the deposit number CCTCC NO: M 2022770, and the deposit address is Wuhan, China. A cold-tolerant Brevibacterium disclosed by the present invention can be made into a deodorant and applied to deodorize the air in poultry houses and manure composting.

The present disclosure provides a method for culture of bacterial strains, comprising the following steps: inoculating a bacterial strain in a first culture medium comprising 0.1 mg / L-0.8 mg / L of 1-bromohexane to obtain a first culture. The method for culture of bacterial strains provided by the present disclosure further comprises: inoculating the first culture into a second culture medium, wherein the second culture medium comprises 0.2 mg / L-0.8 mg / L of 1-bromohexane. The method can be used for fermentation of Gluconacetobacter melanogenum, Propionibacterium, Corynebacterium, Brevibacterium, Bacillus, Gluconobacter, Lactobacillus, Agromyces, Actinobacillus, Escherichia coli, Anaerobiospirillum, Trichoderma, Streptomyces, Aspergillus, Saccharomyces or Myceliopthora, effectively preventing infection of bacteriophages during fermentation.

The invention belongs to the technical field of microorganisms, and particularly relates to streptomyces baubi GZ-10 and application thereof. The preservation number of the streptomyces baubi GZ-10 is GDMCC NO.65684, the strain name is Streptomyces boili, and the streptomyces baubi GZ-10 is separated from a soil sample in the dolomite area of Guangzhou City, Guangdong Province, is obtained through separation and purification by adopting a Gause No.1 agar culture medium, has red-based internal hyphae and white aerial hyphae, and can produce red soluble pigments. The streptomyces baubi disclosed by the invention has broad-spectrum bacteriostatic activity on various pathogenic bacteria and putrefying bacteria; according to the invention, antibacterial metabolites generated by the streptomyces baubi are brevibacterium peptide and cinereorubicin B; the streptomyces is simple in culture condition and easy for industrial production, and has potential application value in the field of microbial source preservatives.

The therapeutic or healthful aspect of nutritional supplements, dietary supplements, and drugs has been significantly improved synergistically through infusion of paraprobiotics (inactivated or attenuated or killed probiotics) along with their immunomodulins or postbiotics, especially those of genus Lactobacillus, Bacillus, Lactococcus, Pediococcus, Propionibacterium, Enterococcus, Streptococcus, Penicillium, and Brevibacterium. The objection of using live probiotic bacteria as adjuvants in combination with the nutraceuticals, dietary supplements and drugs by the medical practitioners has been obviated through use of paraprobiotics along with their postbiotics to improve health or treat a specific disease.

The present invention belongs to the technical field of agricultural microorganisms, and in particular, relates to a cold-resistant Brevibacterium with the function of promoting growth and increasing production, a composite bacterial agent, and a preparation method and application thereof. The cold-resistant Brevibacterium is deposited in the China Center for Type Culture Collection with a deposit number of CCTCCM2023822. Cold-resistant Brevibacterium has the functions of nitrogen fixation, iron carrier production or growth factor production; at the same time, when it is used in combination with Sinorhizobium meliloti Z12 and Vibrio vulgaris Cb1, it has the beneficial effects of promoting alfalfa growth, reducing the application of chemical fertilizers, and increasing the yield and quality of alfalfa. It is an environmentally friendly multifunctional composite bacterial agent with good development prospects.

The invention relates to the technical field of organic pollutant treatment, in particular to a method for degrading aged petroleum hydrocarbon pollutants in soil. Firstly, low-temperature dielectric barrier plasma treatment with specific parameters is utilized, and a preliminary degradation effect is performed according to the characteristics of the aged petroleum hydrocarbon pollutants; and secondly, by utilizing specific types of compound bacteria microorganisms and by virtue of a good synergistic effect among the pseudomonas aeruginosa, the microbacterium oxydans and the ochrobactrum intermedium in the compound bacteria microorganisms, the degradation efficiency and the degradation rate of the microorganisms on the primary degradation substances are obviously improved, and an intermediate degradation product with smaller molecular weight is generated. In addition, after the compound bacteria microorganisms are introduced, through control of specific conditions, deep degradation of the compound bacteria microorganisms on intermediate degradation products is achieved, and the total degradation rate is greatly increased. Through low-temperature dielectric barrier plasma treatment and'different space coupling 'of compound bacteria and microorganisms, a new idea is provided for treating pollutants through the coupling synergistic effect of the low-temperature dielectric barrier plasma treatment and the compound bacteria and microorganisms.

The invention relates to a hexavalent chromium removing bacterium Brevibacterium otidis XM1 and application thereof, and relates to the technical field of environmental microorganisms, in particular to a hexavalent chromium removing bacterium Brevibacterium otidis XM1 and application thereof. The strain is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M 20242532, and the 16S rDNA sequence of the strain is as shown in SEQ ID NO: 1. The strain is separated from an industrial polluted environment, is obtained through gradient domestication and screening, and has high tolerance and efficient reducing capacity to Cr (VI). Under the conditions that the pH is 7.0 and the temperature is 37 DEG C, the removal rate of 50 mg / L Cr (VI after 48 hours reaches 97.87%, and the removal rate of 72 hours reaches 100%. 90% of Cr (VI) in the industrial wastewater can be removed within 72 hours under the conditions that the pH is 4.0 and the temperature is 45 DEG C. The strain can be applied to biological treatment of chromium-containing wastewater, and is low in cost and free of secondary pollution.

Provided in the present application is the use of an Alzheimer's-disease intestinal flora biomarker combination in the preparation of a product for detecting AD. The marker combination comprises: any one of or a combination of at least two of Akkermansia muciniphila, Turicibacter, Dubosiella, Pantoea, Alistipes, Lactobacillus, Erysipelothrix, Lachnospira, Enterobacter, Corynebacterium, Clostridium, an undefined genus in Erysipelotrichaceae, Brevibacterium or Odoribacter. In the present invention, the abundance level of the intestinal flora biomarker combination is used as a detection index for early auxiliary diagnosis of Alzheimer's disease.

The therapeutic or healthful aspect of nutritional supplements, dietary supplements, and drugs has been significantly improved synergistically through infusion of paraprobiotics (inactivated or attenuated or killed probiotics) along with their immunomodulins or postbiotics, especially those of genus Lactobacillus, Bacillus, Lactococcus, Pediococcus, Propionibacterium, Enterococcus, Streptococcus, Penicillium, and Brevibacterium. The objection of using live probiotic bacteria as adjuvants in combination with the nutraceuticals, dietary supplements and drugs by the medical practitioners has been obviated through use of paraprobiotics along with their postbiotics to improve health or treat a specific disease.

This invention enables accurate measurement of greenhouse gas emissions from animals. It also allows for easy evaluation of the reduction effect of greenhouse gas emissions from animals. Furthermore, it provides a methane emission inhibitor that suppresses methane production from Methanobrevibacter bacteria. [Solution] A bacterial quantification method for quantifying Methanobrevibacter species contained in the rectal feces of animals includes the steps of: collecting rectal feces from an animal; holding the rectal fecal sample in an anaerobic porter under anaerobic conditions; replacing the gas in the anaerobic porter with nitrogen gas, diluting the sample with a culture medium, and culturing the bacteria in a test tube; recovering the cultured bacteria; extracting DNA from the recovered bacteria; and quantifying the Methanobrevibacter species from the extracted DNA by quantitative PCR or a gene analysis method using next-generation sequencing analysis.