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Brevibacterium aureum AN3 and use thereof in degrading malachite green and other dyes

A malachite green, Brevibacterium technology, applied in bacteria, microorganism-based methods, microorganisms and other directions, can solve the problems of incomplete degradation, no discovery of Brevibacterium aureus degrading dyes, etc., to achieve improved efficiency, good market application prospects, improved The effect of ecological environment quality

Inactive Publication Date: 2009-04-22
GUANGDONG INST OF MICROORGANISM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of these bacteria can only partially degrade one dye under anaerobic conditions
[0003] Brevibacterium aureus has not been found to have the function of degrading dyes

Method used

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  • Brevibacterium aureum AN3 and use thereof in degrading malachite green and other dyes
  • Brevibacterium aureum AN3 and use thereof in degrading malachite green and other dyes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Take 5mL of activated sludge from an anaerobic baffle reactor system used by a research institute in Guangzhou to treat printing and dyeing wastewater in 50mL of dye medium (1.0g of yeast extract per liter of medium, (NH4) 2 SO 4 2.5g, KH 2 PO 4 13.3g, Na 2 HPO 4 21.6g, and add triphenylmethane, so that the dye concentration in the medium is 50mg / L, the rest is water), 30 ℃ static culture, observe the decolorization of the dye, when the dye in the culture medium is basically completely decolorized , transferred to another fresh same medium with 10% inoculum size, and repeated several times until the decolorization time was significantly shortened, and the culture solution was properly diluted and evenly spread on the LB solid medium (containing Peptone 10g / L, yeast extract 5g / L, NaCl 10g / L, agar strip 2%, pH7.0), cultured at 30°C for 24h, picked a single colony for isolation and purification, and isolated and purified the single colony with LB The liquid medium (wi...

Embodiment 2

[0028] Add Na to 200mL deionized water 2 HPO 4 ·7H 2 O 2.56g, KH 2 PO 4 0.6g, NaC 0.1g, NH 4 Cl 0.2g, malachite green GR (C.I.42000) 0.01g, sterilized at 211°C for 20 minutes as dye culture solution. Inoculate the slant surface of the bacterial strain AN3 obtained in Example 1 into LB liquid medium, activate the thalline on a shaker at 30°C and 150r / min, and make the number of bacteria reach 10 8 Order of magnitude, inoculate the activated bacteria solution in the dye culture solution with an inoculation amount of 1 / 10, and culture it statically at 30°C, observe the color change of the culture solution, take 10mL of the dye culture solution every 30 minutes and centrifuge at 6000r / min to remove the bacteria , using a DU640 ultraviolet spectrophotometer (BECKMAN) to measure the absorbance value of malachite green GR (C.I.42000) in the dye culture solution at 617nm, and taking the dye culture solution without adding bacteria as a control, the degradation rate of the dye was...

Embodiment 3

[0030] Add Na to 200mL deionized water 2 HPO 4 ·7H 2 O 2.56g, KH 2 PO 4 0.6g, NaCl 0.1g, NH 4 Cl 0.2g, crystal violet (C.I.42555) 0.01g, sterilized at 211°C for 20 minutes as a dye culture solution. Inoculate the slant surface of the bacterial strain AN3 obtained in Example 1 into LB liquid medium, activate the thalline on a shaker at 30°C and 150r / min, and make the number of bacteria reach 10 8 Order of magnitude, inoculate the activated bacterial solution in each dye culture solution with an inoculation amount of 1 / 10, culture at 30°C, observe the color change of each culture solution, take 10mL dye culture solution every 30 minutes and centrifuge at 6000r / min Bacteria were removed, and the absorbance of crystal violet (C.I.42555) in the dye culture solution was measured at 590nm with a DU640 ultraviolet spectrophotometer (BECKMAN), and the dye degradation rate was calculated using the corresponding dye culture solution without bacteria as a control. The obtained stra...

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Abstract

The invention relates to a new strain golden Brevibacterium strain AN3 CGMCC No.2193, and application thereof in degrading triphenyl methane dyes and azo dyes such as malachite green. The golden Brevibacterium strain AN3 is obtained by separating and purifying activated sludge in an anaerobic baffled reactor system used for treating printing and dyeing wastewater by an research institute in Guangzhou, can degrade the triphenyl methane dyes in a mode of benzene ring detoxication under a facultative anaerobic condition that the temperature is between 25 and 37 DEG C and the pH value is between 6.0 and 8.5, and has significant capability on degrading the prior triphenyl methane dyes and azo dyes such as the malachite green which bring about serious pollution to aquatic products and are used in the form of a fungicide, wherein the removal rate of the golden Brevibacterium strain to the malachite green with the concentration of 50 milligrams per liter is 61.4 percent in 8 hours and is 97.3 percent in 16 hours, and the highest tolerable concentration thereof is 500 milligrams per liter, so that the strain golden Brevibacterium strain can effectively solve the problems of pollution and purification caused by the malachite green in an aquaculture environment.

Description

technical field [0001] The invention relates to a new strain of Brevibacterium aureum (Brevibacterium aureum) AN3 and its application in degrading triphenylmethane dyes such as malachite green and azo dyes. Background technique [0002] Dye molecule is a kind of artificially synthesized complex aromatic hydrocarbon compound. According to the statistics of C.I. (Color Inder) in the United States, there are as many as 10,000 kinds of dyes currently in use. They not only have specific colors, but also have complex structures and low biodegradability. To" effect. These synthetic dyes are widely used in a variety of industries. Due to their complex structure and slow natural degradation rate, they cause serious pollution to the water environment. Therefore, the treatment of residual dyes in the environment is one of the hot and difficult points of current research. Studying the biodegradation characteristics of chemical dyes is conducive to the correct use of microorganisms to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/28C12R1/13
Inventor 任随周孙国萍曾国驱陈亮张培培卫晋波陈进林
Owner GUANGDONG INST OF MICROORGANISM
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