561 results about "Muscle tissue" patented technology

The invention provides a massage robot control method and system, a medium, a program product and a terminal. Through a massage robot control algorithm of global active planning and local passive planning, personalized, accurate, safe and effective massage treatment is realized. A massage path and a target area are defined by global active planning, and the overall massage direction and range are determined. Passive planning is adopted locally, and the robot adjusts in real time according to feedback and physical conditions of a patient. The self-adaptive technology is adopted, massage parameters and force are adjusted in real time, and the optimal massage effect is ensured. Compared with a traditional massage robot, the treatment precision and safety are improved, and the safety risk caused by the fact that the robot is not suitable for individual differences is reduced. Meanwhile, through application of the self-adaptive technology, the massage effect is balanced and optimized, and the technical defects that an existing massage robot achieves accurate control and adapts to the body types and muscle tissue characteristics of different patients are overcome.

The invention relates to a skin tissue layering evaluation method, device and equipment, a medium and a program product. The method comprises the following steps: acquiring an original gray-scale ultrasonic image of skin tissue layering, and preprocessing the original gray-scale ultrasonic image to obtain a preprocessed ultrasonic image; performing elastic image generation on the preprocessed ultrasonic image through an elastic imaging generation network to obtain an elastic modulus diagram; performing super-resolution image reconstruction on the preprocessed ultrasonic image through a super-resolution reconstruction network to obtain a high-resolution image; performing feature extraction on the preprocessed ultrasonic image, the elastic modulus diagram and the high-resolution diagram to obtain a multi-modal hierarchical feature map; performing cross-modal attention fusion on the multi-modal hierarchical features to obtain a fusion feature map; and according to the fusion feature map, performing layering evaluation on the skin tissue layering to obtain a multi-skin tissue probability map. By adopting the method, the integrated skin tissue layering evaluation of the whole body skin, fascia and muscular tissues can be realized under the limitation of insufficient resolution.

A cushion promotes healthy blood flow within the body when the patient is sitting / lying down. A pressure controlling support automatically balances user weight, detects application of user weight, or otherwise detects when the user is at-risk for pressure sores. Pressure is dynamically redistributed by inflation / deflation of individual bladders, rebalancing user support. The cushion prevents the user from applying their weight to impede their blood flow at an at-risk location or from cramping their musculature. The cushion also massages the user's musculature, by moving a pressure difference about selected bladders, promoting blood flow and reducing seating fatigue. The cushion detects changes in pressure at each bladder, to detect / predict user fidgeting / movement and adjust pressure in response thereto, to support the user's new position.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to growth traits of litopenaeus vannamei and application of the SNP molecular marker, and belongs to the field of molecular marker-assisted breeding of aquatic animals. The genome DNA of the muscular tissue of the litopenaeus vannamei to be detected is extracted as a template, the gene is subjected to PCR amplification and sequencing, the genotype of the molecular marker SNP1-3 is determined, and the molecular marker SNP1-3 is used as the SNP molecular marker for seed selection, so that the aim of quickly screening litopenaeus vannamei individuals with obvious growth advantages in the early stage is fulfilled. According to the molecular marker related to the growth traits of the litopenaeus vannamei, the breeding period can be greatly shortened, the fine breed breeding process of the litopenaeus vannamei is accelerated, the breeding efficiency is improved, and the breeding cost is reduced.

The invention discloses a knee joint replacement post-operation fixing and pressurizing training belt and system. The knee joint replacement post-operation fixing and pressurizing training belt comprises a waist belt, a knee assembly, a foot assembly, a first elastic belt and a second elastic belt. The two sides and the rear portion of the waistband are provided with hooks to be connected with a first elastic belt and connected with the upper end of the knee assembly through the first elastic belt, and the two sides of the lower end of the knee assembly are provided with hooks to be connected with a second elastic belt and connected with the tiptoe component and the heel component of the foot assembly through the second elastic belt. All the components work coordinately, so that comprehensive exercise of knee joints and peripheral muscular tissues of the knee joints is realized; adjusting assemblies are arranged in the knee assemblies, and the training intensity can be adjusted; in addition, each part of the device is provided with an attitude sensor, real-time monitoring of foot and leg motions is realized, motion data is recorded and simulated and analyzed through a monitoring computer on the waistband, user feedback and training guidance are provided, a patient is helped to adjust a training scheme according to personal conditions, rehabilitation training is scientifically carried out, and the recovery process is accelerated.

The invention discloses a cerebral apoplexy patient upper limb rehabilitation evaluation method based on electroencephalogram and electromyographic signals. The method specifically comprises the following steps that signals are collected and preprocessed; electroencephalogram and electromyographic signal decomposition; performing multi-scale processing; calculating transmission spectrum entropy; and upper limb rehabilitation grade evaluation: mapping a fitting relationship between the MSTSE significance area value of each patient and a Brunnstrom evaluation scale based on a Spearman correlation coefficient, analyzing an FCMC characteristic index and a change trend which are significantly related to the grade of the Brunnstrom evaluation scale, and performing quantitative evaluation on the disease grade of the stroke patient. According to the technical scheme, a binary variational mode decomposition-multi-scale transfer spectrum entropy method is used for representing the FCMC coupling relation between the brain motor cortex and the corresponding muscular tissue, and in combination with a Brunnstrom rating scale, objective quantitative evaluation of the rehabilitation level of the upper limbs of the stroke patient is achieved.

The invention relates to the technical field of biology, and provides a probiotic composition for relieving sarcopenia obesity and application of the probiotic composition. The composition comprises at least two of lactobacillus plantarum, lactobacillus rhamnosus and bifidobacterium animalis subsp. Lactis. Wherein the preservation number of the lactobacillus plantarum is CGMCC (China General Microbiological Culture Collection Center) No.25839; the preservation number of the lactobacillus rhamnosus is CGMCC (China General Microbiological Culture Collection Center) No.26968; the preservation number of the animal bifidobacterium subsp. Lactis is CGMCC (China General Microbiological Culture Collection Center) No. 27998. The composition disclosed by the invention can be used for increasing the muscle content of an organism, improving the muscle tissue form, increasing adenosine triphosphate and inhibiting abnormal increase of body weight, so that the composition can be used for effectively preventing and treating sarcopenia obesity.

The invention relates to the technical field of medical biological materials, in particular to an aquatic animal source acellular matrix and a preparation method and application thereof. The aquatic animal source acellular matrix is prepared by performing pretreatment and acellular treatment on connective tissues of aquatic animals; the pretreatment method comprises the following steps: carrying out enzymolysis for 12-60 hours by using biological enzyme at the temperature of 0-15 DEG C, and then removing muscular tissues, basilar membranes and epidermal layers to obtain the miscellaneous tissue-free dermal tissues. The aquatic animal-derived acellular matrix provided by the invention has a bionic structure similar to or consistent with animal original tissues, does not contain components possibly causing immune risks, and is high in clinical safety and effectiveness.

The present invention provides a dance leg-kicking training device and a training method. The device includes a support frame, a rolling member, a traction rope, a leg fixing member, a driving mechanism and a sensor assembly. The rolling member is arranged on the support frame. The first end of the traction rope is wound around the rolling member, and its second end is connected to the leg fixing member. The driving mechanism is arranged on the support frame and connected to the rolling member, and is used for driving the rolling member to roll, so as to contract or extend the traction rope, and further lift or lower the leg. The sensor assembly is arranged at a relative interval with the support frame and electrically connected to the driving mechanism, and is used for controlling the driving mechanism to be in a static state when the leg kicks to a preset angle, so that the leg can stay at the preset angle, which can relax the muscle tissue of the thigh root and achieve the training effect of making the legs slender and straight. The present invention standardizes the action requirements and training skills of ground leg-kicking training through the dance leg-kicking training device, and improves the safety and autonomous training efficiency of leg-kicking training.

A muscle probe is provided for obtaining electromyography data and optical spectroscopy data from muscle tissue. The muscle probe comprises an elongate needle having an outer wall surrounding a needle interior, the needle interior comprising: a core electromyography electrode; and one or more optical fibres; wherein the needle is arranged to be inserted into a muscle, and further arranged to detect electrical activity from the muscle; and wherein the one or more optical fibres are arranged to direct incident light from a light source toward a target area of the muscle, and further arranged to receive scattered light from the target area. The present disclosure aims to provide a muscle probe to improve the diagnostic pathway for patients with neuromuscular disorders, by developing a minimally invasive bedside test of muscle health.

A testing device for determining a stiffness of a patient's tissue is provided herein that includes a probe housing, an internal probe housed in the probe housing, a load cell connected to the internal probe, and a linear-actuator connected to the load cell. The patient's tissue may be joint tissue, cervical tissue, uterine tissue, muscular tissue, and / or pulmonary tissue. The internal probe may be configured to translate within the probe housing, and the linear-actuator may be configured to move the load cell, causing translation of the internal probe within the probe housing. When the load cell is moved by the linear-actuator, the internal probe may translate within the probe housing, and a tip of the internal probe may cause indentation into a patient's tissue.

The invention relates to the field of pelvic floor muscle recovery treatment, in particular to a female pelvic floor muscle recovery treatment device and an adjusting structure thereof.The female pelvic floor muscle recovery treatment device comprises a movable base, a control box is arranged on the surface of the movable base, a hydraulic rod is installed on one side of the control box, and a recovery supporting plate is installed at the top of the hydraulic rod; a massage repairing device capable of massaging female pelvic floor muscles is installed on the upper surface of the repairing supporting plate, a movable structure is installed in the repairing supporting plate, and a lifting adjusting structure capable of freely moving is arranged above the repairing supporting plate. The top of the lifting adjusting structure is provided with an abdomen repairing structure capable of repairing female abdomen muscle tissue, and the matrix type layout of the massage vibrators can stimulate pelvic floor muscle groups in a partitioned mode, so that the biological feedback effect is enhanced. The leg repairing structure drives a sliding ball to move in an arc groove through an electric telescopic rod, switching between resistance training and passive stretching is achieved, and lower limb venous thrombosis is effectively prevented.

The invention discloses a pig muscle fiber geometric phenotype and spatial distribution characteristic quantification method, and relates to the technical field of cell image information extraction, and the technical key points are that the method comprises the steps: measuring the shape index of muscle fibers through contour data; measuring an overall distribution mode of muscle fibers through contour and type data; measuring the difference between the muscle fiber distribution modes of the two skeletal muscle dyeing images through the contour data and the type data; and analyzing spatial distribution characteristics according to the muscle fiber phenotypic data and the spatial position. The problem that in the prior art, a measurement scheme for the geometric phenotype and phenotype space distribution characteristics of the muscle fibers is lacked is solved, and automatic, systematic and integrated efficient calculation is achieved. The method is expected to provide a rich phenotypic measurement basis for analyzing skeletal muscle phenotypic differences of different pig breeds and different parts, phenotypic association of meat quality and muscle fibers, and a spatial regulation mechanism of cell gene expression in pig muscle tissues.

A system and method for controlling an orthosis. The system comprises: a pressure detection module (2), configured to detect pressure information of an orthosis pressure applying zone; a pressure control module (3), configured to adjust the pressure information applied by an orthosis (8) according to a received pressure adjustment instruction; a blood oxygen detection module (1), configured to detect blood oxygen information of the orthosis pressure applying zone; a temperature detection module (4), configured to detect temperature information of the orthosis pressure applying zone; a temperature control module (5), configured to adjust the temperature information applied by the orthosis (8) according to a received temperature adjustment instruction; a control platform, configured to determine whether to trigger the pressure adjustment instruction and the temperature adjustment instruction or not according to one or more of the received pressure information, blood oxygen information, and temperature information. The system can monitor the skin and muscle tissue conditions in the orthosis pressure applying zone in real time and provide early warning of abnormal conditions. Moreover, the system has a low cost and is worn comfortably and suitable for long-term wearing and daily monitoring.

The invention discloses a method for researching the repairing effect of low-pressure and low-oxygen exercise on waist polyfidus injury, which comprises the following steps: S1, selecting and grouping experimental animals: selecting healthy adult rats, and randomly dividing the healthy adult rats into a plurality of experimental groups including a conventional exercise group, a low-pressure and low-oxygen exercise group, a low-pressure and low-oxygen treatment group and a control group; s2, setting a low-pressure and low-oxygen exercise environment: simulating a high-altitude environment by using a low-pressure oxygen cabin; the treadmill is used for rat running exercise; and S3, exercise plan implementation: performing exercise training on all the rats in the experimental group on the five-channel running machine. According to the invention, exercise training is carried out in a low-pressure and low-oxygen environment, so that a new physical intervention means is provided for rehabilitation treatment of waist muscle injury; researches show that movement under low-pressure and low-oxygen conditions is beneficial to acceleration of regeneration and repair processes of muscular tissues, which is crucial for rapid recovery of normal functions after acute or chronic waist injury.

The present invention provides a method for tenderizing eliminated laying hens with alternating dual-frequency ultrasound, comprising the following steps: pre-treating the breast meat of eliminated laying hens, and then using a tenderizer to perform coordinated ultrasound treatment, to obtain the result; the tenderizer includes calcium chloride and protease; the ultrasound is alternating dual-frequency ultrasound (22 / 40kHz). The ultrasonic device of the present invention adopts a concentric circle arrangement mode of ultrasonic vibrators, which can provide an external uninterrupted alternating dual-frequency ultrasonic physical field for the entire tenderizing process of eliminated laying hens, and maximize the penetration of calcium chloride and protease solution into the deep layer of muscle tissue. The former calcium chloride can increase the ion strength in the muscle, improve the ion distribution, and enhance the water retention of muscle fibers; the latter protease hydrolyzes and refines the muscle fibers, disintegrates the macromolecular protein of the muscle fibers, and reduces the shear force, thereby improving the tenderness of the eliminated laying hens.

The invention discloses SNP (Single Nucleotide Polymorphism) molecular markers of a Penaeus vannamei HIF-1alpha gene. The SNP molecular markers are respectively positioned at basic groups of the 67th site, the 119th site, the 133rd site, the 172nd site, the 340th site, the 409th site, the 437th site, the 474th site, the 577th site, the 647th site and the 749th site of a nucleotide sequence shown in a sequence 1 in a sequence table. The molecular marker is used as a functional marker of the nitrite-resistant character of the penaeus vannamei for breeding, specifically, genome DNA of muscular tissue of the penaeus vannamei to be detected is extracted, then the obtained genome DNA is used as a template for PCR amplification and purification of a PCR amplification product, then the obtained PCR amplification product is sequenced, and the content of the nitrite-resistant character of the penaeus vannamei is determined. The genotype of the molecular marker is determined, so that dominant genotype individuals are selected for breeding the penaeus vannamei boone, and a penaeus vannamei boone strain with good nitrite-resistant character can be obtained.

The invention belongs to the field of biology, and discloses a method for identifying RNA (Ribonucleic Acid) binding protein in porcine intramuscular fat cells or subcutaneous fat cells, which comprises the following steps: step 1, taking porcine muscle tissues or subcutaneous fat tissues to carry out meat mashing and digestion, and collecting mature intramuscular fat cells or subcutaneous fat cells; step 2, washing intramuscular fat cells or subcutaneous fat cells, and carrying out cross-linking treatment by adopting ultraviolet irradiation; step 3, collecting intramuscular fat cells or subcutaneous fat cells after cross-linking treatment, and performing lysis under the action of a cell lysis solution to obtain a lysis solution; step 4, adding magnetic beads into the lysate, and carrying out repeated resuspension and elution to obtain an eluent; and 5, separating the RNA binding protein from the magnetic beads, and incubating. According to the method, intramuscular fat cells or subcutaneous fat cells are treated by adopting a UV cross-linking solidification method, and then RNA binding protein is captured through magnetic beads, so that the RNA binding protein is successfully obtained.

The invention provides a method for detecting fluorine compounds in shrimps, which comprises the following steps: S1, sample preparation: removing shells of to-be-detected shrimps, and crushing and homogenizing muscular tissues of the to-be-detected shrimps to obtain a to-be-detected sample; s2, extraction: extracting a sample to be detected, retaining an organic phase, and concentrating to obtain a concentrated solution; then adding water which is 5-15 times of the concentrated solution for dilution to obtain a to-be-purified solution; the extraction solvent is an aqueous solution of an organic solvent; s3, purification: purifying the to-be-purified liquid by using a solid-phase extraction column to obtain a purified liquid; s4, concentration and constant volume: concentrating the purified liquid to be nearly dry, redissolving, and filtering to obtain filtrate; and S5, sample detection: detecting the filtrate by using a liquid chromatograph-mass spectrometer, and carrying out qualitative and quantitative analysis on the fluorine compounds in the prawns by adopting a standard curve method. The method can be used for simultaneously determining the contents of 41 PFASs compounds with different physicochemical properties in shrimps.

The invention provides a muscle state evaluation method, ultrasonic detection equipment and a storage medium. The method comprises the following steps: acquiring a first ultrasonic echo signal of a target muscle tissue under a contraction condition and / or a relaxation condition; determining a first parameter value of at least one target ultrasonic quantitative parameter according to the first ultrasonic echo signal, wherein the target ultrasonic quantitative parameter is an ultrasonic quantitative parameter related to a muscle state; based on a muscle state prediction model, obtaining a first index value of a target evaluation index according to the first parameter value of the at least one target ultrasonic quantitative parameter; wherein the target evaluation index at least comprises an index representing that the target muscle tissue is normal or abnormal; and obtaining a state evaluation result of the target muscle tissue according to the first index value of the target evaluation index.

The disclosure provides anti-myostatin antibodies and methods of making and using the same. Nucleic acids encoding the anti-myostatin antibodies and host cells comprising the nucleic acids are also provided. The anti-myostatin antibodies have uses that include treating a muscle wasting disease, reducing body fat accumulation, and increasing mass and strength of muscle tissue. The disclosure also provides polypeptides containing a variant Fc region and methods of making and using the same. Nucleic acids encoding the polypeptides and host cells comprising the nucleic acids are also provided. The polypeptides have uses that include suppressing the activation of immune cells; treating an immunological inflammatory disease, autoimmune disease, or viral infection; and increasing muscle mass and strength or reducing body fat accumulation.

The invention provides a tendinopathy rehabilitation training and monitoring system based on electrical stimulation, and the system comprises a detection unit which is used for measuring the electrical impedance of the tendon and muscular tissue of a patient before rehabilitation training, and providing a basis for the initial setting of electrical stimulation parameters; the electrical stimulation unit is used for monitoring the muscle contraction degree, the motion angle and the angular velocity, the blood oxygen metabolism condition of a tendon area and the pressure condition around muscles of the rehabilitation person under electrical stimulation in the rehabilitation training process. A muscle electrophysiological sensor, a joint angle sensor, a near infrared spectrum sensor, a motion capture sensor, a pressure pad sensor and the like are utilized, rehabilitation training conditions can be monitored from multiple aspects, a more detailed rehabilitation training report is generated in combination with various weight ratios, targeted training can be carried out on different individuals, various parameters can be adjusted, and the rehabilitation training efficiency is improved. The training effect is improved, training information can be fed back in time, and doctors can conveniently carry out whole-course coordination management on training.

The present invention relates to the field of biotechnology and discloses a probiotic composition for alleviating sarcopenic obesity and its use. The composition comprises at least two of Lactobacillus plantarum, Lactobacillus rhamnosus, and Bifidobacterium animalis subsp. lactis; wherein the Lactobacillus plantarum has a deposit number of CGMCC No. 25839; the Lactobacillus rhamnosus has a deposit number of CGMCC No. 26968; and the Bifidobacterium animalis subsp. lactis has a deposit number of CGMCC No. 27998. The composition of the present invention can increase muscle mass, improve muscle tissue morphology, increase adenosine triphosphate, and inhibit abnormal weight gain, thereby effectively preventing and treating sarcopenic obesity.

Systems and methods can be implemented in which an electromagnetic charge is used to strengthen, tone, and firm muscle tissues. For example, the method may include receiving parameters for operation of a magnetic muscle stimulation device. The method may also include causing, based on the received parameters, a first alternating current having a first phase to flow through a first coil and generate a first time-varying magnetic field that induces a first electric charge in muscle tissue under a skin surface of a patient when the first applicator surface is positioned substantially on and parallel to the skin surface of the patient. The method may also include causing a second alternating current having a second phase to flow through a second coil in a similar manner. Optionally, a phase offset between the first and second phases may be adjusted to limit to a predetermined maximum electric charge.

The invention discloses a stomach cancer patient postoperative muscle recovery process visual monitoring platform and a multi-terminal cooperation method, and the method comprises the following steps: setting an initial scanning layer thickness for scanning according to the specific condition of a patient before CT examination; the CT scanning equipment is used for scanning and imaging the cross section of the postoperative muscular tissue of a gastric cancer patient, continuous dynamic image data are obtained by collecting scanning layer data in real time, and the real-time change condition in the muscular tissue is recorded. According to the intelligent prediction result of the convolutional neural network, the CT scanning layer thickness is automatically optimized in real time, and particularly, the scanning layer thickness is actively reduced when a high-risk gas sign is found, so that the image acquisition precision is remarkably improved, an abnormal gas region with relatively small volume and relatively low density in postoperative muscular tissue is better captured, and the postoperative muscular tissue quality is improved. Therefore, the space average effect and the missed diagnosis risk caused by traditional fixed layer thickness scanning are remarkably reduced.

The prostate detection device comprises a top frame, a Y-axis electric air cylinder is embedded in an inner cavity of the top frame, a piston rod of the Y-axis electric air cylinder is fixedly connected with a support, a top barrel is transversely fixed to the top, close to an alarm lamp, of the support, and bottom covers are fixedly connected to the two sides, away from the top barrel, of the support; a sampling assembly for prostate tissue fluid puncture is arranged on the top cylinder, and a sample mixing assembly for uniformly mixing the prostate tissue fluid and a detection reagent is arranged on the bottom cover. Firstly, tissue fluid samples in prostate tissues and muscle tissues are punctured and sampled through the piston head, and after tissue fluid sampling work of prostate pathological samples is completed, the tissue fluid samples and detection reagents in the two groups of reaction barrels are fully and uniformly mixed and react, so that various substances in the tissue fluid samples are fully separated out; subsequent detection work such as direct smear microscopic examination, smear staining microscopic examination and tetrazole nitrogen blue test is facilitated, and the comprehensiveness and accuracy of prostate detection are improved.

Disclosed herein are hydrogels that can be loaded with myogenic agents, for instance muscle stem cells (MuSCs) / satellite cells, pro-myogenic factors, and combinations thereof. The hydrogels can be contacted with damaged muscle tissue, thereby facilitating muscle growth and repair. Further provided are methods of repairing muscle tissue in a patient in need thereof, said methods comprising contacting the muscle tissue with a composition comprising the hydrogel. Also provided herein are kits comprising the hydrogel composition and a substrate comprising at least one microneedle.

The invention provides application of alpha-ketobutyrate in preparation of drugs for prevention and / or treatment of skeletal muscle atrophy, and belongs to the technical field of biomedicine.The human endogenous metabolite alpha-ketobutyrate is applied to prevention and treatment of skeletal muscle atrophy, the side effect is small, the safety is high after long-term use, and the effect is obvious. Serious immunoreaction or toxic reaction can be avoided, and skeletal muscle senescence and muscular atrophy can be effectively prevented and treated. The alpha-ketobutyrate can improve the muscle index of skeletal muscles of the old mice, increase the number of muscle fibers, increase the cross sectional area, increase the number of muscle cell nucleuses and the expression of nuclear fiber layer protein LamB1 of the muscle cell nucleuses, and can also increase the capillary angiogenesis rate in muscle tissues of the old mice. Alpha-ketobutyrate has good pharmacological characteristics and low side effects, and provides an important basis for developing clinical drugs for treating amyotrophy.

The invention discloses combined probiotics for improving sarcopenia as well as a preparation method and application of the combined probiotics, and belongs to the field of probiotics. The invention relates to a combined probiotic for improving sarcopenia. The combined probiotic comprises a lactobacillus reuteri body, a lactobacillus johnsonii body, a lactobacillus fermentum body and a bifidobacterium adolescentis body. The combined probiotics disclosed by the invention can be used for remarkably improving the overall symptoms of sarcopenia model mice, including inhibiting weight loss, constipation symptoms and colonic mucosa destruction, prolonging load swimming time, improving muscle endurance, improving skeletal muscle atrophy, improving the expression of I-type slow muscle fiber protein MyHC I and promoting slow muscle fiber repair, and has the effects of preventing and treating sarcopenia. The invention discloses a probiotic intervention method for treating sarcopenia, which comprises the following steps of: preparing probiotics for treating sarcopenia, reducing the content of inflammatory cytokines in muscular tissues, increasing the content of hepatic glycogen, improving systematic metabolism of mice with sarcopenia and damaged serum markers, including lactic acid, lactic dehydrogenase, creatine kinase, creatinine, alanine transaminase ALT and aspartate transaminase AST, providing a new scheme for probiotic intervention for sarcopenia, and being used for preparing related functional products or intervention means.

The invention relates to the technical field of food processing and storage and transportation preservation, in particular to a three-section variable-temperature liquid nitrogen quick-freezing preservation method for beef and application, and the method comprises the following steps: S1, performing pretreatment such as trimming, fascia removal and pre-cooling on beef; s2, performing three-stage variable-temperature liquid nitrogen quick freezing to enable the central temperature of the meat to reach-18 DEG C; and S3, after the sample is frozen and stored for 28 days, performing quality detection and analysis. The three-stage variable-temperature liquid nitrogen quick freezing provided by the invention can effectively reduce the freezing time and cooking loss of the beef, delay the spoilage of proteins and lipids, maintain a better muscle tissue form, and maintain the fresh appearance characteristic of the beef.