284 results about "Muscle tissue" patented technology

A muscle probe is provided for obtaining electromyography data and optical spectroscopy data from muscle tissue. The muscle probe comprises an elongate needle having an outer wall surrounding a needle interior, the needle interior comprising: a core electromyography electrode; and one or more optical fibres; wherein the needle is arranged to be inserted into a muscle, and further arranged to detect electrical activity from the muscle; and wherein the one or more optical fibres are arranged to direct incident light from a light source toward a target area of the muscle, and further arranged to receive scattered light from the target area. The present disclosure aims to provide a muscle probe to improve the diagnostic pathway for patients with neuromuscular disorders, by developing a minimally invasive bedside test of muscle health.

The invention provides a method for detecting fluorine compounds in shrimps, which comprises the following steps: S1, sample preparation: removing shells of to-be-detected shrimps, and crushing and homogenizing muscular tissues of the to-be-detected shrimps to obtain a to-be-detected sample; s2, extraction: extracting a sample to be detected, retaining an organic phase, and concentrating to obtain a concentrated solution; then adding water which is 5-15 times of the concentrated solution for dilution to obtain a to-be-purified solution; the extraction solvent is an aqueous solution of an organic solvent; s3, purification: purifying the to-be-purified liquid by using a solid-phase extraction column to obtain a purified liquid; s4, concentration and constant volume: concentrating the purified liquid to be nearly dry, redissolving, and filtering to obtain filtrate; and S5, sample detection: detecting the filtrate by using a liquid chromatograph-mass spectrometer, and carrying out qualitative and quantitative analysis on the fluorine compounds in the prawns by adopting a standard curve method. The method can be used for simultaneously determining the contents of 41 PFASs compounds with different physicochemical properties in shrimps.

The invention discloses combined probiotics for improving sarcopenia as well as a preparation method and application of the combined probiotics, and belongs to the field of probiotics. The invention relates to a combined probiotic for improving sarcopenia. The combined probiotic comprises a lactobacillus reuteri body, a lactobacillus johnsonii body, a lactobacillus fermentum body and a bifidobacterium adolescentis body. The combined probiotics disclosed by the invention can be used for remarkably improving the overall symptoms of sarcopenia model mice, including inhibiting weight loss, constipation symptoms and colonic mucosa destruction, prolonging load swimming time, improving muscle endurance, improving skeletal muscle atrophy, improving the expression of I-type slow muscle fiber protein MyHC I and promoting slow muscle fiber repair, and has the effects of preventing and treating sarcopenia. The invention discloses a probiotic intervention method for treating sarcopenia, which comprises the following steps of: preparing probiotics for treating sarcopenia, reducing the content of inflammatory cytokines in muscular tissues, increasing the content of hepatic glycogen, improving systematic metabolism of mice with sarcopenia and damaged serum markers, including lactic acid, lactic dehydrogenase, creatine kinase, creatinine, alanine transaminase ALT and aspartate transaminase AST, providing a new scheme for probiotic intervention for sarcopenia, and being used for preparing related functional products or intervention means.

The invention discloses application of an FGF9 gene in regulation and control of growth and development of chicken skeletal muscles, and further discloses application of an overexpression vector of the FGF9 gene or an interference fragment of the overexpression vector in promotion or inhibition of proliferation and differentiation of chicken myoblasts. The invention also discloses a specific primer pair for detecting the expression quantity of the FGF9 influencing the growth and development of the chicken skeletal muscle. The invention also discloses a detection kit and a detection method thereof. The detection kit provided by the invention can be used for detecting the expression quantity of the FGF9 gene in chicken muscle tissues and myoblasts, and the detection method is simple and quick. The FGF9 gene can be used as an important gene for researching a chicken skeletal muscle growth and development regulation theory.

Systems and methods can be implemented in which an electromagnetic charge is used to strengthen, tone, and firm muscle tissues. For example, a magnetic muscle stimulation device may be provided that includes a processor and a device applicator having a housing and a coil positioned within in the housing. The processor may be configured to receive parameters for operation of the device applicator, and to cause, based on the received parameters, an alternating current to flow through the coil to generate a time-varying magnetic field that induces an integrated electric charge or electrical conductivity in a portion of tissue under a skin surface of a patient.

The invention discloses a CircSESN1 gene as well as an overexpression vector, a specific primer pair, a detection kit and application of the CircSESN1 gene. The invention also discloses application of the CircSESN1 gene, an overexpression vector, the specific primer pair or the fluorescent quantitative detection kit in regulation and control of growth and development of chicken skeletal muscle. The invention also discloses a method for inhibiting chicken myoblast proliferation and / or promoting differentiation. The detection kit provided by the invention can be used for detecting the expression quantity of the CircSESN1 gene in chicken muscle tissues and myoblasts, and the detection method is simple and quick. The gene can be used as an important non-coding RNA for researching a regulation theory of growth and development of chicken skeletal muscles.

The present invention provides novel capsid variants for viral vectors that simultaneously detarget liver tissue and efficiently transduce muscular tissue.

The present invention provides a production method for a three-dimensional muscle tissue including the steps of: preparing an approximately rectangular first cell module containing skeletal myoblasts in a hydrogel, and having a plurality of approximately rectangular holes parallel to each other, and an approximately rectangular second cell module containing skeletal myoblasts in a hydrogel, and having a plurality of approximately rectangular holes parallel to each other at positions different from those of the first cell module in a vertical direction; alternately stacking the prepared first cell module and the prepared second cell module to obtain a stack; subjecting the skeletal myoblasts contained in the obtained stack to proliferation culture; and inducing the proliferated skeletal myoblasts to differentiate into myotubes.

The present disclosure provides double stranded ribonucleic acid (dsRNA) agents for inhibiting expression of a target gene, comprising an antisense strand which is complementary to the target gene; a sense strand which is complementary to the antisense strand and forms a double stranded region with the antisense strand; at least one alpha-v-beta-6 (αvβ6) integrin targeting ligand that mediates delivery to muscle tissue conjugated to at least one strand; and at least one in vivo delivery enhancing moiety conjugated to one or more internal positions on at least one strand. The present disclosure also provides compositions comprising such dsRNA agents, and methods of use thereof for treating a subject having a disorder that would benefit from reduction in expression of the target gene.

CT images from the neck, lung, cardiac, abdominal, pelvis, hip, spine or lower extremity areas are obtained and analyzed by a computer to measure trabecular bone density on each level it is available, in the spine and hip. Any bone tissue associated with vertebrae that is fractured is excluded, along with Schmorl's nodes, large blood vessels, and cortical bone, to accurately average the trabecular bone density on a scan. The density of fat, heart, and muscle tissue of a subject is used to calibrate the results. That data may be input to an absolute fracture risk model (Fracture Risk Algorithm calculator). Information such as bone density, T score, Z score, fracture risk, and identification of vertebral fractures (if present) may be determined and reported.

The present invention relates to the use of GRP78 and peptides similar thereto or nucleic acids encoding the same. The peptides or nucleic acids according to the present invention can increase the expression of biomarkers having reduced expression in senescent cells, and thus can be used to restore the function of muscle tissue that has decreased in function due to aging, etc.

Disclosed are a method for creating a surgical training model, a surgical training model apparatus, a bone model, an article that emulates tissue of an animal musculoskeletal system, an article that emulates animal fat tissue, and an article that emulates animal skin tissue. One version of the method comprises placing a spinal vertebrae model in a cavity model that emulates an animal body cavity; forming a first layer on top of the vertebrae model, wherein the first layer emulates an animal muscle tissue; placing a second layer over the first layer, wherein the second layer emulates an animal fat tissue; and placing a third layer over the second layer, wherein the third layer emulates an animal skin tissue. The spinal vertebrae model can be 3D printed from a thermoplastic polymer and infiltrated with a foam into an interior space of the 3D printed spinal vertebrae model.

The invention discloses an overexpression vector of LAMP2B and application thereof, which can effectively delay the disease progress of DMD, and comprises: (a) an AAV vector which is an adeno-associated virus vector and has high muscle tissue targeting and low liver tropism; (b) a CMV (cytomegalovirus) promoter, wherein the CMV promoter is a cytomegalovirus promoter; (c) a coding sequence of an hLAMP2B gene, namely a coding sequence of a human LAMP2B gene, and the coding sequence of the hLAMP2B gene is operably linked to the CMV promoter; wherein the vector is configured to overexpress LAMP2B protein in muscular tissue, and the vector is self-complementary AAV, namely scAAV, the concept is ingenious, the novel AAV vector with high muscle transformation efficiency is utilized to deliver a complete LAMP2B gene (LAMP2B is a gene related to autophagy regulation) into muscle cells of all organs of a mouse (AAV-hLAMP2B for short), and the autophagy regulation function of the mouse is improved. The muscle tissue function of the mouse DMD disease model is comprehensively assisted to recover, and the disease progress of the DMD is delayed.

The invention belongs to the technical field of biology, and discloses a preparation method of an intestinal plexus patch, which comprises the following steps: killing an intestinal tract donor, fixing intestinal tissue segments of duodenum, jejunum, ileum or colon by using paraformaldehyde, and dehydrating to obtain dehydrated intestinal tissue segments; washing the dehydrated intestinal tissue section and removing mesentery, fat and Pari's lymph nodes attached to the jejunum, cutting an intestinal mass of about 4-6mm perpendicular to the intestinal tissue section, flatly laying the intestinal mass on a glass slide, dropwise adding a PBS (Phosphate Buffer Solution) of Triton X-100, fixing the intestinal mass by using a smooth surface of a pair of tweezers, stripping muscle tissues along the annular muscle direction, and drying the intestinal mass in a drying oven. The intestinal slices with intermuscular nerve plexus are obtained; finally, the intestinal piece with the intermuscular nerve plexus is cleaned, then fluorescent staining and piece sealing are conducted, and the mouse intestinal nerve plexus patch is obtained. The preparation method of the mouse intestinal nerve plexus patch has the advantages of being convenient, rapid and the like.

The disclosure relates to compositions and methods for enhancing asymmetric division of satellite cells and promoting muscle cell / tissue regeneration, for treatment of muscle tissue injuries and muscle diseases, including muscular dystrophies.

The utility model discloses a thawing device of tilapia processing, including support frame still including the deflector that makes fish body slide and the deflector of surface of installation in support frame, the baffle of fixed connection on deflector, the intercepting assembly of installation on baffle and intercept fish body, thawing assembly of installation on baffle, the liquid collecting assembly of installation on support frame and the guide assembly of installation on liquid collecting assembly, fish body moves on deflector, and thawing assembly is used for spraying water to fish body, and the wastewater of thawing is collected through liquid collecting assembly, and the fish body of thawing is finished and passes through the guide assembly empty part moisture while from the liquid collecting assembly top. The deflector of ladder form makes fish body slide under the action of gravity and turns over naturally, ensures that each surface of fish body can evenly receive thawing water, effectively eliminates "thawing dead angle", avoids the forced turning of rigid parts such as mechanical arm, prong in whole process, maximum degree reduces muscle tissue damage, and can guarantee the continuity of thawing process.

A composition has hyaluronic acid and one or more materials as an admixture. The hyaluronic acid is derived from a fascia tissue layer of an alligator, the fascia layer located below a hide and above muscle tissue. The one or more materials as the admixture to the hyaluronic acid can be a carrier, diluent or excipient. The hyaluronic acid is extracted from the fascia tissue layer in the form of an oil having an oily viscosity with a molecular weight of 30,000 or greater. The oil extracted includes the hyaluronic acid and includes sodium or salts of hyaluronic acid. The oil extracted is anti-inflammatory to human tissue.

This invention relates to molecular marker 1769 and its application in the identification of sex-reversed pseudomale individuals in the Northeast Forest Frog (Rana davidii), and pertains to the field of molecular markers. To address the current difficulty in identifying sex-reversed pseudomale individuals in the Northeast Forest Frog, this invention extracts the genome from the muscle tissue of the left hind limb of the Northeast Forest Frog. Using the extracted genomic DNA as a template, PCR amplification is performed using primer pairs containing the sequences shown in SEQ ID NO:1 and SEQ ID NO:2. The PCR amplification products are detected by electrophoresis on a 3% agarose gel. Phenotypic male individuals exhibiting the 1769 bp band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are genetically male Northeast Forest Frogs, i.e., true males. Phenotypic male individuals lacking the band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are pseudomale individuals who have undergone sex reversal from genetically female to phenotypically male. Phenotypic female individuals lacking the band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are female individuals.

The invention relates to the technical field of training equipment, in particular to muscle training equipment which comprises a seat, a plurality of connecting assemblies and a plurality of electromagnetic assemblies. The connecting assemblies are installed on the seat, the electromagnetic assemblies are arranged on the connecting assemblies and move the corresponding connecting assemblies, the multiple connecting assemblies are combined to wrap the legs, and the electromagnetic assemblies generate an electromagnetic field acting on the legs after being powered on; through cooperative use of the connecting assemblies and the electromagnetic assemblies, when a trainee uses the device, the legs are placed among the connecting assemblies, and electromagnetic waves and high-frequency electromagnetic energy are generated after the electromagnetic assemblies are powered on, so that blood vessels are rapidly expanded, blood circulation is accelerated, oxygen and nutrient substances are rapidly conveyed to muscular tissue, sufficient energy is provided for muscles, and the training effect is improved. The muscle strength is enhanced, the muscles can quickly enter a good working state, and the training effect is guaranteed for people who perform high-strength physical training.

The application discloses a kind of screening methods and application of pivot gene related to sheep muscle fatty acid content, to solve the technical problems that local sheep breed sample quantity is limited, traditional single gene analysis method is difficult to analyze fatty acid metabolism regulation from network level.This application carries out transcriptome sequencing to multiple months of muscle tissue of Gangba sheep, constructs gene expression matrix, using weighted gene co-expression network analysis (WGCNA) Combined with module characteristic gene and fatty acid phenotype correlation screening strategy, the pivot gene significantly positively correlated with muscle fatty acid content is obtained.The screening method can construct a robust co-expression network under limited sample size, systematically identify the functional module and core gene related to the content of fatty acids such as linoleic acid, and reveal the dynamics of fatty acid metabolism at different ages, and the method can be extended to other plateau livestock;The screened pivot gene can be used as a molecular breeding marker for early selection of Gangba sheep, and the breeding cycle is shortened.

The invention discloses a muscle tissue information extraction method, system and device and a medium, and relates to the technical field of image analysis. Comprising the steps of obtaining a muscular tissue slice image; key features of each pixel point in the slice image are extracted, and the probability value of each pixel point is calculated according to the key features; classifying the slice images according to a probability value calculation result to obtain the number of muscle cells and the total area of muscle tissues, and calculating the average area and the average diameter of each muscle cell; extracting the muscular fascia area in the slice image, subtracting the muscular fascia area from the total area of the muscular tissues and then subtracting the total area of the muscle cells to obtain the sum of the areas of the endometrium, and calculating the thickness of the endometrium of the muscle cells according to the area of the endometrium; integrating the number, the average area, the average diameter and the thickness of the endometrium of the muscle cells to complete information extraction of the muscle tissue. According to the method, the dependence on manual operation is reduced, and the information of the muscle cells can be rapidly and accurately extracted.

This invention provides a method and device for displaying muscle tissue morphology based on a digital twin of the levator scapulae muscle. The method includes acquiring static magnetic resonance imaging data of the target user's shoulder and neck region, multi-angle dynamic optical motion capture data, and surface electromyography (EMG) signals of the target muscle group to construct multimodal physiological data of the levator scapulae muscle. Based on the static magnetic resonance imaging data, a static anatomical base model of the levator scapulae muscle is constructed using image segmentation and 3D reconstruction techniques. According to the multi-angle dynamic optical motion capture data and EMG signals, the activation sequence and corresponding activation intensity of the target nerve driving the contraction of the levator scapulae muscle under different movements are calculated. Based on the activation sequence and corresponding activation intensity of the target nerve, the static anatomical base model of the levator scapulae muscle is embedded into a pre-trained digital twin model of the levator scapulae muscle to obtain a target digital twin of the levator scapulae muscle. The input motion command is input into the target digital twin of the levator scapulae muscle, and the muscle tissue morphology results are output and displayed.

This invention relates to the molecular marker M-513 and its application in the identification of sex-reversed pseudomale individuals in the Northeast Forest Frog (Rana davidii), and pertains to the field of molecular markers. To address the current difficulty in identifying sex-reversed pseudomale individuals in the Northeast Forest Frog, this invention extracts the genome from the muscle tissue of the left hind limb of the Northeast Forest Frog. Using the extracted genomic DNA as a template, PCR amplification is performed using primer pairs containing the sequences shown in SEQ ID NO:1 and SEQ ID NO:2. The PCR amplification products are detected by electrophoresis on a 3% agarose gel. Phenotypic male individuals exhibiting a 513bp band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are genetically male Northeast Forest Frogs, i.e., true males. Phenotypic male individuals lacking the band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are pseudomale individuals exhibiting sex reversal from genetically female to phenotypically male. Phenotypic female individuals lacking the band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are female individuals.

The present disclosure provides compositions capable of delivering a load (including therapeutic agents, e.g., RNA) to organs or tissues other than the liver, lung and spleen (e.g., brain, heart, kidney and muscular tissue). More specifically, the composition comprises a plurality of lipid particles comprising an agent, a cationic lipid, and an ionizable lipid.

The invention provides a targeted mRNA delivery system of muscle tissues and / or muscle cells based on aptamer functionalized LNP (Apt-LNP), the delivery system can significantly improve mRNA transfection of the muscle tissues, and meanwhile, mRNA expression in main organs such as liver and spleen is greatly reduced. The expression intensity of muscle tissue in-situ mRNA is improved through modification of the aptamer, meanwhile, mRNA expression in main organs such as the liver and the spleen is greatly reduced, and side effects caused by off-target of mRNA infectious disease vaccines are expected to be avoided. The method is expected to be applied to the field of infectious disease mRNA vaccines The invention also provides a targeting mRNA delivery system of lymph node tissues and / or immune cells based on the aptamer functionalized LNP (Apt-LNP). Compared with an existing lymph node targeting system, the Apt-LNP modified by the aptamer not only can realize lymph node mRNA targeting, but also can further target immune cells, and is expected to be applied to the field of tumor mRNA vaccines to enhance the curative effect of tumor vaccines.

The invention discloses a method for separating, culturing and identifying porcine muscle-derived mesenchymal stem cells and fat progenitor cells. The method mainly comprises the following steps: dissociating pig muscle tissues through a tissue processor, obtaining mesenchymal stem cells and adipose progenitor cells by utilizing a flow cytometry sorting technology, and completing in-vitro culture and identification of the cells. According to the technical scheme provided by the invention, the technical problems of primary culture, separation and identification of the mesenchymal stem cells and the adipose progenitor cells of the porcine muscle tissue are effectively solved, and a reliable method is provided for further application of a flow cytometry sorting technology and a myogenic mesenchymal stem cell and adipose progenitor cell primary model in animal nutrition research.

Implantable devices and / or sensors can be wirelessly powered by controlling and propagating electromagnetic waves in a patient's tissue. Such implantable devices / sensors can be implanted at target locations in a patient, to stimulate areas such as the heart, brain, spinal cord, or muscle tissue, and / or to sense biological, physiological, chemical attributes of the blood, tissue, and other patient parameters. The propagating electromagnetic waves can be generated with sub-wavelength structures configured to manipulate evanescent fields outside of tissue to generate the propagating waves inside the tissue. Methods of use are also described.

A massage device is provided. [Solution] The massage device comprises an inner housing (10), a rotary motor mounted in the inner housing and having an output shaft parallel to a first direction, an eccentric connection assembly (30) connected to the output shaft, and a massage head (40) connected to the eccentric connection assembly and arranged generally at an angle relative to the first direction. When a user uses the massage device, they place a cover body (62) over their chest and then start the device. The eccentric connection assembly causes the entire massage head to rotate eccentrically, and simultaneously the massage head vibrates, so that when the massage head comes into contact with the chest, it kneads and vibrates the chest, strengthening stimulation to muscle tissue and achieving a better massage effect.

The present invention disclosure relates to a rapid and cost-effective method for the isolation of genomic material from muscle tissues of mangrove crab for the molecular detection of bacterial pathogens. The methods increase the total recovery of genomic DNA from muscle tissues by heating to 100oC in addition to mechanical disruption of tissues by homogenization. The extracted and isolated genomic DNA using the present invention can 10 be used for the molecular detection of bacterial pathogens from muscle tissues of mangrove crab.