The application provides a neurovascular
coupling imaging
system, method, device and storage medium, which comprises an image collector, a
microscope frame arranged in the light inlet direction of the image collector, a visible light
system for emitting visible light to a sample to be collected after the visible light is reflected by a
beam splitter in the
microscope frame, a near-
infrared light
system for emitting near-
infrared light to the sample to be collected after the near-
infrared light is reflected by another
beam splitter in the
microscope frame, and a trigger device for switching the frequency of the time-sharing emission of each
light source in the visible light system and the near-infrared light system based on the collection frequency of the image collector. The application can integrate different visible light or near-infrared
laser light sources in the light path to be collected, is compatible with various imaging requirements such as visible light for imaging of nerve
cell activity and near-infrared light
speckle imaging for non-invasive
blood flow imaging, ensures that there is no
time difference between image collection and
light source switching and no
time resolution is lost, and facilitates subsequent storage and calculation of data.