The invention discloses a
laser heat effect evaluation method and device, and belongs to the technical field of
biology. The method comprises the following steps: acquiring an original
laser speckle
blood flow image sequence, and analyzing an original
laser speckle image by adopting a time contrast analysis
algorithm to obtain a laser
speckle contrast image; and converting the laser
speckle contrast image to obtain a laser speckle
blood flow image. Calculating the
full width at half maximum of the
blood vessel in the laser speckle
blood flow image as the width of the
blood vessel; subtraction is conducted on the blood flow image obtained after laser
irradiation and the blood flow image obtained before laser
irradiation, and a difference image is obtained; blood vessels with blood flow changes due to laser
irradiation are obtained according to the difference image, then a
region of interest is selected, the blood flow mean value of the blood vessels before and after laser irradiation is obtained, and therefore the final
laser treatment effect is evaluated. The mouse spine window 108 is irradiated from the complete
skin side of the mouse spine window 108, and the blood flow change is monitored in real time on the opposite exposed
blood vessel side by adopting technologies such as laser speckle blood
flow imaging and the like. The influence of the upper
skin tissue is considered, the blood flow change is monitored in real time, and accurate evaluation of the blood vessel laser
heat effect is achieved.