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653 results about "White Spots" patented technology

White balance processing method directed towards atypical-feature image

InactiveCN102883168AVersatileExtensive processing effectsColor signal processing circuitsInternal memoryBright spot
The invention relates to a white balance processing method directed towards an atypical-feature image. The method comprises the following steps: (1) acquiring the brightest spot RGB (Red, Green and Blue) information of a frame of images as well as an RGB mean through a Camera Link industrial camera; (2) scanning the frame of video images, setting a white spot brightness threshold value and an RGB channel difference threshold, and determining whether current images contain a white area or not and color is rich or not; (3) performing color space conversion on the current images if the current images are determined that the white region is not contained and the color is not rich, and respectively calculating an average chromaticity Cw_ave of a reference point of a perfect reflection method and an average chromaticity Ch_ave of a reference point of a gray world method; and (4) calculating an average chromaticity Cw_ave obtained by the perfect reflection method and an average chromaticity Ch_ave obtained by the gray world method directed towards the current images, and performing white balance correction combined with two classic algorithms on the current frame of images by using the ratio of the Cw_ave and (the Cw_ave plus the Ch_ave) as an adjustment factor K. The method can be realized by using the recourses of an FPGA (Field Programmable Gata Array) internal logic unit, an internal memory, a multiplier and the like.
Owner:SHANGHAI UNIV

Method for studying structural diversity of daqu bacterial community

InactiveCN102382877AAvoid the downside of losingReveal and recognize diversityMicrobiological testing/measurementCommunity structureMicrobial ecology
The invention relates to a method for studying the structural diversity of a daqu bacterial community, i.e. the denaturing gradient gel electrophoresis (DGGE) technology, which belongs to the technical field of microbial ecology and mainly comprises the following steps that: 1), daqu genomic deoxyribonucleic acid (DNA) is directly extracted; 2), bacterial universal primers are selected, and specific DNA fragments in the bacterial ribosome DNA are amplified; 3), polymerase chain reaction (PCR) products are separated through DGGE; 4), corresponding strips of microbes in the DGGE fingerprint map are recovered in a gel cutting way; 5), PCR is carried out again, products are connected to a T carrier, the blue and white spot screening is carried out, and positive clone verification is carried out; and 6), the species information of the corresponding microbes of the DGGE strips is obtained through the sequence test. The method does not rely on the molecular biology technology of the traditional culture method, has the characteristics of sensitivity, convenience and accuracy and solves the difficult problems to study some microbes incapable of being cultured or difficult to culture, and the method provides the technical basis for the verification of the daqu bacterial community structure and the discovery of new wine brewing microbes or functional microbes.
Owner:CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD

Vacuum infusion and flow guide system and infusion and flow guide method for reinforcing plate of wind turbine blade

The invention discloses a vacuum infusion and flow guide system and an infusion and flow guide method for a reinforcing plate of a wind turbine blade. The vacuum infusion and flow guide system comprises a reinforcing plate mold (1), a core material (2) mounted on the reinforcing plate mold (1), a flow guide net (3) laid on the glass fiber upper skin of the core material (2) and a flow guide groove omega tube (4) laid on one side of the reinforcing plate mold (1), wherein a glue injection port (5) is formed in the flow guide groove omega tube (4), a spiral tube (6) is mounted on the opposite side of the reinforcing plate mold (1), a suction port (7) is connected to the spiral tube (6), and bi-directional shallow slots (2-1) are formed in the upper surface and the lower surface of the core material (2). The vacuum infusion and flow guide system is reasonable in structural design, resin flows on the upper surface and the lower surface uniformly and isocratically through opposite side drainage, the technical defect of white spots formed on the surface due to the fact that local annular loop is formed because glue solution flows downwards to the lower surface can be overcome, the mechanical strength of the prepared reinforcing plate of the blade is high, the firm degree is high, and the service life is long.
Owner:LIANYUNGANG ZHONGFU LIANZHONG COMPOSITES GRP

Fluorescence quantitative PCR (Polymerase Chain Reaction) detection method for porcine transmissible gastroenteritis virus gene S and primer thereof

The invention discloses a fluorescence quantitative PCR (Polymerase Chain Reaction) detection method for a porcine transmissible gastroenteritis virus gene S and a primer thereof in the technical field of biotechnology. The method comprises the following steps of: cloning a PCR amplification target segment identified as a positive PCR product to a vector pMD18-T, transforming to a competent cell DH5alpha, selecting positive clone by screening blue and white spots and identifying sequencing; extracting a positive recombinant plasmid, quantifying by using an ultraviolet spectrophotometer, diluting a standard product series by 10 times of gradient until the final concentration is 1.0*10<3>-1.0*10<11> copies/mL, undergoing a fluorescence quantitative PCR by taking the standard product series as a template, and establishing a fluorescence quantitative PCR standard curve; and extracting virus RNA (Ribonucleic Acid) of a clinical excrement sample, undergoing a fluorescence quantitative PCR, and calculating the content of viruses in the sample according to a result and the standard curve, wherein the sequences of the primer are sequence 1 and sequence 2. The method and the primer have theadvantages that: a fluorescent probe does not need to be designed additionally, the cost is lowered, operation is easy and convenient, and detection can be completed within 2 hours. The detection method and the primer are suitable for any fluorescence quantitative PCR instrument, and can be applied to the detection of large-scale and high-flux samples.
Owner:SHANGHAI JIAO TONG UNIV
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